pycom01g16420

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0013092
Physical Location & Seq
Reverse (-)
16001964 .. 16004927
2964 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 621 bp
ATGGCAGCCGTAGACAAAGCCCACGTGGTTGTTGATGTGGAGGAGGGGGAAGTGGGACGCCCCAGCGCCGCCACGGGTCGACGTAGCTACCCTGAGTCCGGCTCCGAGGGGTCGGAGGATGGGGAGAAGAGGTGCTCCAGTGAGTCAGGGTCTGAGATCGTGGAGAGGGAATCATCTGCGTCGGAGAGTTCAGTGGAGGTGGATCTGGAGGAGGTGAAGATGCACGTGCCGAAAGTGGAGAGGGATTGCAGGATTTGTCACCTGAGCATGGATGCAACGAATCATGAATCTGGGATTCCTATTGAGCTAGGGTGTTCTTGTAAAGCTGATTTGGCTGCTGCCCACAAGCAGTGTGCTGAGGCTTGGTTCAAGATCAAGGGTAACAAAACCTGTGAGATATGTGGATCAACTGCACGTAATGTGTCTGGTTTGAGTGAAGCTGAGTCGGCGGAGCAGTGGCATGATGAAGCCACACTGGCAGCAGCAGTAGCAGGTGCCCCTGTGCAGGCACCAGAGAGCCGAAACTTCTGGCAGGGTCACCGCTTCCTGAACTTCTTACTAGCTTGTATGGTCTTTGCATTTGTCATCTCGTGGCTGTTTCACTTCAATGTGCCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

207

Amino Acids

22.27

Weight (kDa)

4.97

Isoelectric Point (pI)

56.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 83 - 134 9.7e-11 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013092)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05830
fragaria_vesca FvH4_7g23740 FvH4_7g23740
malus_domestica MD01G1144600.v1.1 MD07G1211200.v1.1
prunus_persica Prupe.2G244000_v2.0.a1
pyrus_communis pycom01g16420 pycom07g19470
rosa_chinensis RchiOBHm_Chr1g0369041
rosa_laevigata RLG00000027162
rosa_multiflora Rmu_sc0004250.1_g000028
rosa_roxburghii Rroxscaffold_4G00288060
rosa_rugosa Rorug01G0347000
rosa_samantha Rh1AG354200 Rh1BG317600 Rh1CG332100 Rh1DG348500
rosa_wichuraiana Rw1G031290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 482
Acc36I ACCTGC 1 cut(s) 482
AccB1I GGYRCC 2 cut(s) 494, 508
AccI GTMKAC 2 cut(s) 12, 79
AciI CCGC 3 cut(s) 69, 449, 541
AclWI GGATC 2 cut(s) 210, 412
AcvI CACGTG 2 cut(s) 25, 226
AcyI GRCGYC 1 cut(s) 58
AfiI CCNNNNNNNGG 3 cut(s) 98, 268, 505
AgsI TTSAA 2 cut(s) 370, 607
AluBI AGCT 5 cut(s) 87, 307, 326, 440, 563
AluI AGCT 5 cut(s) 87, 307, 326, 440, 563
Alw21I GWGCWC 1 cut(s) 137
AlwI GGATC 2 cut(s) 210, 412
AlwNI CAGNNNCTG 1 cut(s) 152
ApeKI GCWGC 5 cut(s) 5, 335, 338, 479, 482
AspLEI GCGC 1 cut(s) 68
AsuHPI GGTGA 3 cut(s) 226, 251, 530
BaeGI GKGCMC 2 cut(s) 499, 615
BanI GGYRCC 2 cut(s) 494, 508
BauI CACGAG 1 cut(s) 589
BbrPI CACGTG 2 cut(s) 25, 226
Bbv12I GWGCWC 1 cut(s) 137
BbvCI CCTCAGC 1 cut(s) 357
BbvI GCAGC 5 cut(s) 17, 322, 325, 491, 494
BccI CCATC 1 cut(s) 113
BfaI CTAG 2 cut(s) 308, 560
BfoI RGCGCY 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 482
BglI GCCNNNNNGGC 1 cut(s) 476
BisI GCNGC 6 cut(s) 6, 69, 336, 339, 480, 483
BlsI GCNGC 6 cut(s) 7, 70, 337, 340, 481, 484
BmiI GGNNCC 3 cut(s) 103, 496, 510
BmsI GCATC 2 cut(s) 210, 262
BplI GAGNNNNNCTC 2 cut(s) 86, 118
BpmI CTGGAG 2 cut(s) 121, 227
Bpu10I CCTNAGC 2 cut(s) 263, 357
BsaAI YACGTR 3 cut(s) 25, 226, 416
BsaHI GRCGYC 1 cut(s) 58
BsaJI CCNNGG 2 cut(s) 72, 105
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 268, 505
Bse1I ACTGG 2 cut(s) 138, 480
BseDI CCNNGG 2 cut(s) 72, 105
BseGI GGATG 2 cut(s) 124, 277
BseLI CCNNNNNNNGG 3 cut(s) 98, 268, 505
BseMII CTCAG 5 cut(s) 84, 144, 254, 348, 432
BseNI ACTGG 2 cut(s) 138, 480
BseRI GAGGAG 2 cut(s) 56, 224
BseSI GKGCMC 2 cut(s) 499, 615
BseXI GCAGC 5 cut(s) 17, 322, 325, 491, 494
BseYI CCCAGC 1 cut(s) 62
BsgI GTGCAG 2 cut(s) 396, 524
BshNI GGYRCC 2 cut(s) 494, 508
BsiHKAI GWGCWC 1 cut(s) 137
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 3 cut(s) 98, 268, 505
BsmFI GGGAC 1 cut(s) 69
Bsp1286I GDGCHC 3 cut(s) 137, 499, 615
Bsp143I GATC 4 cut(s) 156, 202, 372, 404
BspACI CCGC 3 cut(s) 69, 449, 541
BspCNI CTCAG 5 cut(s) 85, 145, 255, 349, 433
BspHI TCATGA 2 cut(s) 283, 617
BspLI GGNNCC 3 cut(s) 103, 496, 510
BspMI ACCTGC 1 cut(s) 482
BspPI GGATC 2 cut(s) 210, 412
BspT107I GGYRCC 2 cut(s) 494, 508
BsrI ACTGG 2 cut(s) 138, 480
BssECI CCNNGG 2 cut(s) 72, 105
BssMI GATC 4 cut(s) 156, 202, 372, 404
BssNI GRCGYC 1 cut(s) 58
BssSI CACGAG 1 cut(s) 589
Bst2BI CACGAG 1 cut(s) 589
Bst6I CTCTTC 1 cut(s) 122
BstACI GRCGYC 1 cut(s) 58
BstBAI YACGTR 3 cut(s) 25, 226, 416
BstC8I GCNNGC 1 cut(s) 507
BstDEI CTNAG 5 cut(s) 93, 153, 263, 357, 441
BstDSI CCRYGG 1 cut(s) 72
BstEII GGTNACC 1 cut(s) 536
BstF5I GGATG 2 cut(s) 124, 277
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 4 cut(s) 159, 205, 375, 407
BstMBI GATC 4 cut(s) 156, 202, 372, 404
BstMWI GCNNNNNNNGC 4 cut(s) 332, 446, 476, 488
BstPI GGTNACC 1 cut(s) 536
BstSLI GKGCMC 2 cut(s) 499, 615
BstV1I GCAGC 5 cut(s) 17, 322, 325, 491, 494
BstX2I RGATCY 1 cut(s) 202
BstYI RGATCY 1 cut(s) 202
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 2 cut(s) 124, 277
BtsI GCAGTG 2 cut(s) 356, 461
BtsIMutI CAGTG 5 cut(s) 145, 198, 356, 461, 473
BveI ACCTGC 1 cut(s) 482
Cac8I GCNNGC 1 cut(s) 507
CaiI CAGNNNCTG 1 cut(s) 152
CciI TCATGA 2 cut(s) 283, 617
CfoI GCGC 1 cut(s) 68
CseI GACGC 2 cut(s) 66, 168
CviAII CATG 4 cut(s) 268, 284, 461, 618
DdeI CTNAG 5 cut(s) 93, 153, 263, 357, 441
DpnI GATC 4 cut(s) 158, 204, 374, 406
DpnII GATC 4 cut(s) 156, 202, 372, 404
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
EciI GGCGGA 1 cut(s) 464
Eco72I CACGTG 2 cut(s) 25, 226
Eco91I GGTNACC 1 cut(s) 536
EcoO65I GGTNACC 1 cut(s) 536
FaeI CATG 4 cut(s) 271, 287, 464, 621
FaiI YATR 6 cut(s) 269, 285, 400, 462, 569, 619
FaqI GGGAC 1 cut(s) 69
FatI CATG 4 cut(s) 267, 283, 460, 617
FblI GTMKAC 2 cut(s) 12, 79
Fnu4HI GCNGC 6 cut(s) 6, 69, 336, 339, 480, 483
FokI GGATG 2 cut(s) 131, 284
Fsp4HI GCNGC 6 cut(s) 6, 69, 336, 339, 480, 483
FspBI CTAG 2 cut(s) 308, 560
GlaI GCGC 1 cut(s) 67
GluI GCNGC 6 cut(s) 6, 69, 336, 339, 480, 483
GsaI CCCAGC 1 cut(s) 66
GsuI CTGGAG 2 cut(s) 121, 227
HaeII RGCGCY 1 cut(s) 69
HapII CCGG 1 cut(s) 99
HgaI GACGC 2 cut(s) 66, 168
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 1 cut(s) 58
Hin1II CATG 4 cut(s) 271, 287, 464, 621
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 80
HindII GTYRAC 1 cut(s) 80
HinfI GANTC 7 cut(s) 95, 143, 170, 280, 287, 295, 443
HpaII CCGG 1 cut(s) 99
HphI GGTGA 3 cut(s) 226, 251, 530
Hpy166II GTNNAC 2 cut(s) 13, 80
Hpy188I TCNGA 4 cut(s) 106, 115, 154, 184
Hpy188III TCNNGA 5 cut(s) 206, 284, 370, 547, 618
Hpy8I GTNNAC 2 cut(s) 13, 80
Hpy99I CGWCG 2 cut(s) 84, 184
HpyCH4IV ACGT 4 cut(s) 24, 82, 225, 415
HpyCH4V TGCA 6 cut(s) 223, 249, 275, 413, 505, 578
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 446, 476, 488
HpyF3I CTNAG 5 cut(s) 93, 153, 263, 357, 441
HpySE526I ACGT 4 cut(s) 24, 82, 225, 415
Hsp92I GRCGYC 1 cut(s) 58
Hsp92II CATG 4 cut(s) 271, 287, 464, 621
HspAI GCGC 1 cut(s) 66
Kzo9I GATC 4 cut(s) 156, 202, 372, 404
LmnI GCTCC 3 cut(s) 107, 140, 451
Lsp1109I GCAGC 5 cut(s) 17, 322, 325, 491, 494
LweI GCATC 2 cut(s) 210, 262
MaeI CTAG 2 cut(s) 308, 560
MaeII ACGT 4 cut(s) 24, 82, 225, 415
MaeIII GTNAC 3 cut(s) 257, 380, 536
MalI GATC 4 cut(s) 158, 204, 374, 406
MboI GATC 4 cut(s) 156, 202, 372, 404
MboII GAAGA 2 cut(s) 139, 229
MflI RGATCY 1 cut(s) 202
MhlI GDGCHC 3 cut(s) 137, 499, 615
MlyI GAGTC 3 cut(s) 104, 152, 452
MmeI TCCRAC 2 cut(s) 93, 162
MslI CAYNNNNRTG 1 cut(s) 606
MspI CCGG 1 cut(s) 99
MwoI GCNNNNNNNGC 4 cut(s) 332, 446, 476, 488
NdeII GATC 4 cut(s) 156, 202, 372, 404
NlaIII CATG 4 cut(s) 271, 287, 464, 621
NlaIV GGNNCC 3 cut(s) 103, 496, 510
NmuCI GTSAC 2 cut(s) 257, 536
PagI TCATGA 2 cut(s) 283, 617
PaqCI CACCTGC 1 cut(s) 482
PfeI GAWTC 4 cut(s) 170, 280, 287, 295
PkrI GCNGC 6 cut(s) 7, 70, 337, 340, 481, 484
PleI GAGTC 3 cut(s) 103, 151, 451
PmaCI CACGTG 2 cut(s) 25, 226
PmlI CACGTG 2 cut(s) 25, 226
PpsI GAGTC 3 cut(s) 103, 151, 451
Ppu21I YACGTR 3 cut(s) 25, 226, 416
PspCI CACGTG 2 cut(s) 25, 226
PspEI GGTNACC 1 cut(s) 536
PspFI CCCAGC 1 cut(s) 62
PspN4I GGNNCC 3 cut(s) 103, 496, 510
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 1 cut(s) 202
RseI CAYNNNNRTG 1 cut(s) 606
SalI GTCGAC 1 cut(s) 78
SatI GCNGC 6 cut(s) 6, 69, 336, 339, 480, 483
Sau3AI GATC 4 cut(s) 156, 202, 372, 404
SchI GAGTC 3 cut(s) 104, 152, 452
SduI GDGCHC 3 cut(s) 137, 499, 615
SfaNI GCATC 2 cut(s) 210, 262
SmiMI CAYNNNNRTG 1 cut(s) 606
SsiI CCGC 3 cut(s) 69, 449, 541
SspMI CTAG 2 cut(s) 308, 560
TaiI ACGT 4 cut(s) 27, 85, 228, 418
TaqI TCGA 1 cut(s) 79
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 4 cut(s) 170, 280, 287, 295
TscAI CASTG 5 cut(s) 145, 198, 356, 461, 480
TseFI GTSAC 2 cut(s) 257, 536
TseI GCWGC 5 cut(s) 5, 335, 338, 479, 482
Tsp45I GTSAC 2 cut(s) 257, 536
TspDTI ATGAA 2 cut(s) 300, 480
TspRI CASTG 5 cut(s) 145, 198, 356, 461, 480
XmiI GTMKAC 2 cut(s) 12, 79
XspI CTAG 2 cut(s) 308, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.