Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0012402

Physical Location & Seq

Genomic Coordinates Forward (+)

19639594 .. 19641507

Gene Structure 1914 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom01g21720.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1914 bp

ATGCAATCGAGCGGTTACAACGGCGTTCCTGACTTCTACCCAAACGGGCGATCCATCTCCATCGCCGCAGCCATGAACGGCCACAACCCATCTCAGAACAATCCCTTCCACCGATCCCAGCTCCCCGGCCTGTTCCTCGACCCCACCGCATCCCAGATCGCTCGCCAAACACAATTCCAACCCCAAACCCAAAACCCAGCAGCCGGTCTCATTGGAAAGCGGACGCTCGCGGAGTTCCAAGCCCAGCAGCAGAACCACTACAACCCCCACCAGAATCCGAGCCAAAATCTCTATCTCCGCTCCCTCAAGGCCAGAACTTTCCAGCACTCCTCTCCGATTTCGCCGTTATCTCCGATAGACTTCTCATCTGGGTCATCACTTTCAGGCTCCGAGTCTTCGTTTTCGTCCACCTCGATTTTGCCTCATCAGCGATTCGGGTTGCCTATGCTTCAGCAGCTCCGCCCTCAGCCCATTAACCAGCCAGCACCACAGGCGATGTCGTATGTTAACAATCTGGTTCCCAACAATCCGGTTCAGACCCGAGGTGTGGACTCCGAGAAGATGTTTAGCAACAGGCTGCAAGAGTTGGAGAAACAGCTTCTCGACGATAACGACGAGGACGACGAAGGCGACGCTGTTTCTGTCATTACCAAAACCAACAGCGAGTGGTCCGAGACGATACAGAATCTGATGGGTACGGCAGCTCCGAGTCAGAACCAAAAGCCCGTTTCGCCGTCGCCTACTTCGTCGTCGTCTTCCTCGTCCTCCGTGGCGTCTCCTGCTACCTCCACGTGTTCCAAGCAGTCACTCATGGAAGCCGCGACGGCTATTTCTGATGGGAAATCTGAGGCCGCTATGGAGATCCTCACCCGGATGAATTCGACCCAGGTCGCGAATCCTAGACCCAGTTCAGAACAAAGGCTTCTGGAGTTCATGGGTTTAGCTTTGAAGTCTCGGGTCAACCCGATTGATAACCCGGCTCCGATAGCGGAGCTTTTTAGGCAGGAGCATACCGGGTCGACTCAGTCGCTTTACGAGTTATCTCCGTGTTTCAGACTCGGATTTATGGCCGCTAACCTCGCAATCCTAGAAGCGACCATGACGGACCAATCAGCGACCAGTAAGGTTCATGTGATTGACTTCGACATAGGGCAGGGTGGTCAGTACGTGCTTCTTTTCCACGCGCTCGTCGCGCGTCAGAATGTCAGGCCCGCTGTCGTGAAGATCACCACCGTCGCTGATAATGGCGGGGAGGAGAGGCTGAGAATGGTCCATCAGAAACTGAGTCAAGACGCCCAGCGACTCGGGGTCCGTCTGGAATTCAACGTAGTGAGCCAGAAAATCGGCGACCTGAACCGGGAATCGCTGGGTTGCGAACCGGACGAGCCGATCGCCGTGAATTTCGCTTTCAAACTGTACAGCATGCCGGACGAGAGCGTGTCCATGGACAACCCCCGCGACGAGCTCCTGCGGCGCGTGAAGGGACTGGCGCCGCGTGTGGTGACGCTGGTCGAGCAGGAGCTCAACACCAACACCGCGCCCTTCATGGCGCGCGTAAACGAGTGCTGCGCGTACTACGGAGCCCTGCTGGAGTCGATCGAGGCGACGGTGCCGAGGGAGAACCCGGAGCGAGTCAAGGTGGAGGAGGCGCTGAGTCGGAAGGTAGCGAACTCGGTTGCTTGCGAAGGTAGGGATCGCGTGGAAAGGTGTGAAGTGTTTGGGAAGTGGCGGGCCCGCATGGGGATGGCGGGGTTCGAGTTGAGGCCCATGGGTCCAAACATGACCGAGTCGTTGAAGAATCGACTGGGGTCGGGAAACCGAGTCAACTCGGCGTTCACGGTTAAAGAAGAGAATGGAGGTGTTTGCTTTGGTTGGATAAGCCGCACTCTCACCGTCGCATCCGCTTGGCGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRAS

Protein Analysis

638

Amino Acids

69.94

Weight (kDa)

6.27

Isoelectric Point (pI)

51.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRAS	PF03514	267 - 637	3.3e-104	GRAS domain family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012402)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52510
fragaria_vesca	FvH4_7g30100
malus_domestica	MD01G1206800.v1.1 MD07G1277400.v1.1
prunus_persica	Prupe.2G299400_v2.0.a1
pyrus_communis	pycom01g21720 pycom07g25240
rosa_chinensis	RchiOBHm_Chr1g0378081
rosa_laevigata	RLG00000026473 RLG00000035032
rosa_multiflora	Rmu_sc0001465.1_g000012
rosa_roxburghii	Rroxscaffold_156G00440300 Rroxscaffold_4G00280790
rosa_rugosa	Rorug01G0406200
rosa_samantha	Rh1AG423600 Rh1BG385000 Rh1CG399000 Rh1DG416200
rosa_wichuraiana	Rw1G037360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	1489, 1609
AccBSI	CCGCTC	2 cut(s)	12, 300
AccI	GTMKAC	1 cut(s)	1019
AclWI	GGATC	4 cut(s)	45, 108, 856, 1701
AcoI	YGGCCR	2 cut(s)	79, 1068
AcsI	RAATTY	3 cut(s)	877, 1319, 1399
AcuI	CTGAAG	1 cut(s)	434
AcvI	CACGTG	1 cut(s)	792
AcyI	GRCGYC	3 cut(s)	773, 1293, 1490
AfaI	GTAC	4 cut(s)	697, 1166, 1418, 1574
AfiI	CCNNNNNNNGG	4 cut(s)	203, 547, 1357, 1740
AflIII	ACRYGT	1 cut(s)	791
AgsI	TTSAA	4 cut(s)	949, 1324, 1411, 1795
AhdI	GACNNNNNGTC	1 cut(s)	1307
AjnI	CCWGG	1 cut(s)	885
AluBI	AGCT	8 cut(s)	121, 457, 598, 704, 944, 994, 1465, 1522
AluI	AGCT	8 cut(s)	121, 457, 598, 704, 944, 994, 1465, 1522
Alw21I	GWGCWC	2 cut(s)	1467, 1524
Alw26I	GTCTC	4 cut(s)	212, 668, 780, 957
AlwI	GGATC	4 cut(s)	45, 108, 856, 1701
AlwNI	CAGNNNCTG	2 cut(s)	688, 1282
Ama87I	CYCGRG	3 cut(s)	540, 954, 1304
AoxI	GGCC	8 cut(s)	79, 127, 309, 849, 1068, 1208, 1731, 1763
ApaI	GGGCCC	1 cut(s)	1735
ApeKI	GCWGC	7 cut(s)	68, 200, 247, 454, 577, 701, 1566
ApoI	RAATTY	3 cut(s)	877, 1319, 1399
AspLEI	GCGC	9 cut(s)	1186, 1195, 1476, 1492, 1540, 1552, 1554, 1571, 1651
AspS9I	GGNCC	9 cut(s)	669, 1105, 1209, 1270, 1309, 1731, 1732, 1764, 1772
AsuC2I	CCSGG	6 cut(s)	126, 871, 977, 1015, 1358, 1625
AsuHPI	GGTGA	4 cut(s)	859, 1219, 1513, 1882
AvaI	CYCGRG	3 cut(s)	540, 954, 1304
AvaII	GGWCC	5 cut(s)	669, 1105, 1270, 1309, 1772
BaeGI	GKGCMC	1 cut(s)	1735
BanI	GGYRCC	2 cut(s)	1489, 1609
BanII	GRGCYC	4 cut(s)	1467, 1524, 1585, 1735
BbrPI	CACGTG	1 cut(s)	792
BbsI	GAAGAC	2 cut(s)	387, 747
Bbv12I	GWGCWC	2 cut(s)	1467, 1524
BbvCI	CCTCAGC	1 cut(s)	465
BbvI	GCAGC	7 cut(s)	80, 212, 259, 466, 564, 713, 1553
BccI	CCATC	7 cut(s)	62, 68, 97, 685, 830, 1281, 1738
BceAI	ACGGC	7 cut(s)	37, 94, 328, 714, 718, 840, 1379
BciT130I	CCWGG	1 cut(s)	887
BcnI	CCSGG	6 cut(s)	126, 871, 977, 1015, 1358, 1625
BcoDI	GTCTC	4 cut(s)	212, 668, 780, 957
BfaI	CTAG	2 cut(s)	900, 1088
BfoI	RGCGCY	2 cut(s)	1493, 1652
BglI	GCCNNNNNGGC	1 cut(s)	824
Bme1390I	CCNGG	7 cut(s)	126, 871, 887, 977, 1015, 1358, 1625
Bme18I	GGWCC	5 cut(s)	669, 1105, 1270, 1309, 1772
BmeRI	GACNNNNNGTC	1 cut(s)	1307
BmeT110I	CYCGRG	3 cut(s)	540, 954, 1304
BmgT120I	GGNCC	9 cut(s)	669, 1105, 1209, 1270, 1309, 1731, 1732, 1764, 1772
BmiI	GGNNCC	9 cut(s)	388, 519, 981, 1310, 1491, 1582, 1611, 1733, 1773
BmrFI	CCNGG	7 cut(s)	126, 871, 887, 977, 1015, 1358, 1625
BmrI	ACTGGG	2 cut(s)	900, 1814
BmsI	GCATC	2 cut(s)	158, 1907
BmuI	ACTGGG	2 cut(s)	900, 1814
BoxI	GACNNNNGTC	3 cut(s)	887, 1508, 1807
BpiI	GAAGAC	2 cut(s)	387, 747
BpmI	CTGGAG	2 cut(s)	947, 1610
Bpu10I	CCTNAGC	1 cut(s)	465
BpuEI	CTTGAG	1 cut(s)	290
BpuMI	CCSGG	6 cut(s)	126, 871, 977, 1015, 1358, 1625
BsaAI	YACGTR	2 cut(s)	792, 1168
BsaHI	GRCGYC	3 cut(s)	773, 1293, 1490
BsaI	GGTCTC	1 cut(s)	212
BsaJI	CCNNGG	7 cut(s)	124, 541, 768, 885, 1443, 1613, 1767
BsaWI	WCCGGW	2 cut(s)	529, 1378
BsaXI	ACNNNNNCTCC	2 cut(s)	688, 718
Bsc4I	CCNNNNNNNGG	4 cut(s)	203, 547, 1357, 1740
Bse118I	RCCGGY	1 cut(s)	203
Bse1I	ACTGG	4 cut(s)	906, 1119, 1491, 1809
BseBI	CCWGG	1 cut(s)	887
BseDI	CCNNGG	7 cut(s)	124, 541, 768, 885, 1443, 1613, 1767
BseGI	GGATG	4 cut(s)	149, 879, 1749, 1898
BseLI	CCNNNNNNNGG	4 cut(s)	203, 547, 1357, 1740
BseMII	CTCAG	7 cut(s)	107, 479, 837, 1037, 1253, 1274, 1643
BseNI	ACTGG	4 cut(s)	906, 1119, 1491, 1809
BsePI	GCGCGC	1 cut(s)	1550
BseRI	GAGGAG	3 cut(s)	319, 1268, 1658
BseSI	GKGCMC	1 cut(s)	1735
BseXI	GCAGC	7 cut(s)	80, 212, 259, 466, 564, 713, 1553
BseYI	CCCAGC	5 cut(s)	117, 196, 243, 1296, 1366
Bsh1285I	CGRYCG	2 cut(s)	1392, 1599
BshFI	GGCC	8 cut(s)	81, 129, 311, 851, 1070, 1210, 1733, 1765
BshNI	GGYRCC	2 cut(s)	1489, 1609
BsiEI	CGRYCG	2 cut(s)	1392, 1599
BsiHKAI	GWGCWC	2 cut(s)	1467, 1524
BsiHKCI	CYCGRG	3 cut(s)	540, 954, 1304
BslFI	GGGAC	1 cut(s)	1497
BslI	CCNNNNNNNGG	4 cut(s)	203, 547, 1357, 1740
BsmAI	GTCTC	4 cut(s)	212, 668, 780, 957
BsmBI	CGTCTC	2 cut(s)	668, 780
BsmFI	GGGAC	1 cut(s)	1497
BsnI	GGCC	8 cut(s)	81, 129, 311, 851, 1070, 1210, 1733, 1765
Bso31I	GGTCTC	1 cut(s)	212
BsoBI	CYCGRG	3 cut(s)	540, 954, 1304
Bsp120I	GGGCCC	1 cut(s)	1731
Bsp1286I	GDGCHC	4 cut(s)	1467, 1524, 1585, 1735
Bsp1407I	TGTACA	1 cut(s)	1416
Bsp143I	GATC	8 cut(s)	50, 113, 156, 861, 1224, 1389, 1596, 1693
Bsp19I	CCATGG	2 cut(s)	1443, 1767
Bsp68I	TCGCGA	1 cut(s)	893
BspANI	GGCC	8 cut(s)	81, 129, 311, 851, 1070, 1210, 1733, 1765
BspCNI	CTCAG	7 cut(s)	106, 478, 838, 1036, 1254, 1275, 1644
BspLI	GGNNCC	9 cut(s)	388, 519, 981, 1310, 1491, 1582, 1611, 1733, 1773
BspPI	GGATC	4 cut(s)	45, 108, 856, 1701
BspT107I	GGYRCC	2 cut(s)	1489, 1609
BspTNI	GGTCTC	1 cut(s)	212
BsrBI	CCGCTC	2 cut(s)	12, 300
BsrFI	RCCGGY	1 cut(s)	203
BsrGI	TGTACA	1 cut(s)	1416
BsrI	ACTGG	4 cut(s)	906, 1119, 1491, 1809
BssAI	RCCGGY	1 cut(s)	203
BssECI	CCNNGG	7 cut(s)	124, 541, 768, 885, 1443, 1613, 1767
BssHII	GCGCGC	1 cut(s)	1550
BssMI	GATC	8 cut(s)	50, 113, 156, 861, 1224, 1389, 1596, 1693
BssNI	GRCGYC	3 cut(s)	773, 1293, 1490
BssT1I	CCWWGG	2 cut(s)	1443, 1767
Bst2UI	CCWGG	1 cut(s)	887
Bst4CI	ACNGT	5 cut(s)	1234, 1416, 1609, 1840, 1894
Bst6I	CTCTTC	1 cut(s)	1842
BstACI	GRCGYC	3 cut(s)	773, 1293, 1490
BstAUI	TGTACA	1 cut(s)	1416
BstBAI	YACGTR	2 cut(s)	792, 1168
BstC8I	GCNNGC	9 cut(s)	163, 228, 483, 1212, 1424, 1552, 1681, 1731, 1735
BstDEI	CTNAG	7 cut(s)	93, 465, 846, 1023, 1262, 1283, 1652
BstDSI	CCRYGG	3 cut(s)	768, 1443, 1767
BstF5I	GGATG	4 cut(s)	149, 879, 1749, 1898
BstH2I	RGCGCY	2 cut(s)	1493, 1652
BstHHI	GCGC	9 cut(s)	1186, 1195, 1476, 1492, 1540, 1552, 1554, 1571, 1651
BstKTI	GATC	8 cut(s)	53, 116, 159, 864, 1227, 1392, 1599, 1696
BstMAI	GTCTC	4 cut(s)	212, 668, 780, 957
BstMBI	GATC	8 cut(s)	50, 113, 156, 861, 1224, 1389, 1596, 1693
BstMCI	CGRYCG	2 cut(s)	1392, 1599
BstNI	CCWGG	1 cut(s)	887
BstNSI	RCATGY	1 cut(s)	1426
BstPAI	GACNNNNGTC	3 cut(s)	887, 1508, 1807
BstSCI	CCNGG	7 cut(s)	124, 869, 885, 975, 1013, 1356, 1623
BstSLI	GKGCMC	1 cut(s)	1735
BstV1I	GCAGC	7 cut(s)	80, 212, 259, 466, 564, 713, 1553
BstV2I	GAAGAC	2 cut(s)	387, 747
BstX2I	RGATCY	1 cut(s)	861
BstYI	RGATCY	1 cut(s)	861
BsuRI	GGCC	8 cut(s)	81, 129, 311, 851, 1070, 1210, 1733, 1765
BtgI	CCRYGG	3 cut(s)	768, 1443, 1767
BtgZI	GCGATG	2 cut(s)	46, 509
BtsCI	GGATG	4 cut(s)	149, 879, 1749, 1898
BtuMI	TCGCGA	1 cut(s)	893
Cac8I	GCNNGC	9 cut(s)	163, 228, 483, 1212, 1424, 1552, 1681, 1731, 1735
CaiI	CAGNNNCTG	2 cut(s)	688, 1282
CfoI	GCGC	9 cut(s)	1186, 1195, 1476, 1492, 1540, 1552, 1554, 1571, 1651
Cfr10I	RCCGGY	1 cut(s)	203
Cfr13I	GGNCC	9 cut(s)	669, 1105, 1209, 1270, 1309, 1731, 1732, 1764, 1772
CseI	GACGC	6 cut(s)	232, 641, 762, 1184, 1301, 1513
Csp6I	GTAC	4 cut(s)	696, 1165, 1417, 1573
CviQI	GTAC	4 cut(s)	696, 1165, 1417, 1573
DdeI	CTNAG	7 cut(s)	93, 465, 846, 1023, 1262, 1283, 1652
DinI	GGCGCC	1 cut(s)	1491
DpnI	GATC	8 cut(s)	52, 115, 158, 863, 1226, 1391, 1598, 1695
DpnII	GATC	8 cut(s)	50, 113, 156, 861, 1224, 1389, 1596, 1693
DriI	GACNNNNNGTC	1 cut(s)	1307
EaeI	YGGCCR	2 cut(s)	79, 1068
Eam1104I	CTCTTC	1 cut(s)	1842
Eam1105I	GACNNNNNGTC	1 cut(s)	1307
EarI	CTCTTC	1 cut(s)	1842
EciI	GGCGGA	1 cut(s)	449
Ecl136II	GAGCTC	2 cut(s)	1465, 1522
Eco130I	CCWWGG	2 cut(s)	1443, 1767
Eco24I	GRGCYC	4 cut(s)	1467, 1524, 1585, 1735
Eco31I	GGTCTC	1 cut(s)	212
Eco47I	GGWCC	5 cut(s)	669, 1105, 1270, 1309, 1772
Eco53kI	GAGCTC	2 cut(s)	1465, 1522
Eco57I	CTGAAG	1 cut(s)	434
Eco72I	CACGTG	1 cut(s)	792
Eco88I	CYCGRG	3 cut(s)	540, 954, 1304
EcoICRI	GAGCTC	2 cut(s)	1465, 1522
EcoRI	GAATTC	2 cut(s)	877, 1319
EcoRII	CCWGG	1 cut(s)	885
EcoT14I	CCWWGG	2 cut(s)	1443, 1767
EcoT38I	GRGCYC	4 cut(s)	1467, 1524, 1585, 1735
EgeI	GGCGCC	1 cut(s)	1491
EheI	GGCGCC	1 cut(s)	1491
ErhI	CCWWGG	2 cut(s)	1443, 1767
Esp3I	CGTCTC	2 cut(s)	668, 780
FaqI	GGGAC	1 cut(s)	1497
FauI	CCCGC	6 cut(s)	1219, 1241, 1463, 1722, 1741, 1742
FblI	GTMKAC	1 cut(s)	1019
FokI	GGATG	4 cut(s)	136, 886, 1756, 1885
FriOI	GRGCYC	4 cut(s)	1467, 1524, 1585, 1735
FspBI	CTAG	2 cut(s)	900, 1088
GlaI	GCGC	9 cut(s)	1185, 1194, 1475, 1491, 1539, 1551, 1553, 1570, 1650
GsaI	CCCAGC	5 cut(s)	121, 200, 247, 1300, 1370
GsuI	CTGGAG	2 cut(s)	947, 1610
HaeII	RGCGCY	2 cut(s)	1493, 1652
HaeIII	GGCC	8 cut(s)	81, 129, 311, 851, 1070, 1210, 1733, 1765
HgaI	GACGC	6 cut(s)	232, 641, 762, 1184, 1301, 1513
HhaI	GCGC	9 cut(s)	1186, 1195, 1476, 1492, 1540, 1552, 1554, 1571, 1651
Hin1I	GRCGYC	3 cut(s)	773, 1293, 1490
Hin6I	GCGC	9 cut(s)	1184, 1193, 1474, 1490, 1538, 1550, 1552, 1569, 1649
HinP1I	GCGC	9 cut(s)	1184, 1193, 1474, 1490, 1538, 1550, 1552, 1569, 1649
HincII	GTYRAC	4 cut(s)	508, 961, 1020, 1825
HindII	GTYRAC	4 cut(s)	508, 961, 1020, 1825
HpaI	GTTAAC	1 cut(s)	508
HphI	GGTGA	4 cut(s)	859, 1219, 1513, 1882
Hpy166II	GTNNAC	8 cut(s)	408, 508, 550, 961, 1020, 1558, 1825, 1836
Hpy188III	TCNNGA	8 cut(s)	29, 602, 892, 926, 1219, 1289, 1316, 1812
Hpy8I	GTNNAC	8 cut(s)	408, 508, 550, 961, 1020, 1558, 1825, 1836
HpyAV	CCTTC	6 cut(s)	115, 620, 1474, 1552, 1654, 1679
HpyCH4III	ACNGT	5 cut(s)	1234, 1416, 1609, 1840, 1894
HpyCH4IV	ACGT	3 cut(s)	791, 1167, 1326
HpyCH4V	TGCA	2 cut(s)	4, 580
HpyF3I	CTNAG	7 cut(s)	93, 465, 846, 1023, 1262, 1283, 1652
HpySE526I	ACGT	3 cut(s)	791, 1167, 1326
Hsp92I	GRCGYC	3 cut(s)	773, 1293, 1490
HspAI	GCGC	9 cut(s)	1184, 1193, 1474, 1490, 1538, 1550, 1552, 1569, 1649
KasI	GGCGCC	1 cut(s)	1489
KspAI	GTTAAC	1 cut(s)	508
Kzo9I	GATC	8 cut(s)	50, 113, 156, 861, 1224, 1389, 1596, 1693
Lsp1109I	GCAGC	7 cut(s)	80, 212, 259, 466, 564, 713, 1553
LweI	GCATC	2 cut(s)	158, 1907
MaeI	CTAG	2 cut(s)	900, 1088
MaeII	ACGT	3 cut(s)	791, 1167, 1326
MaeIII	GTNAC	3 cut(s)	14, 804, 1501
MalI	GATC	8 cut(s)	52, 115, 158, 863, 1226, 1391, 1598, 1695
MbiI	CCGCTC	2 cut(s)	12, 300
MboI	GATC	8 cut(s)	50, 113, 156, 861, 1224, 1389, 1596, 1693
MboII	GAAGA	6 cut(s)	387, 571, 747, 1234, 1807, 1859
MflI	RGATCY	1 cut(s)	861
MhlI	GDGCHC	4 cut(s)	1467, 1524, 1585, 1735
MluCI	AATT	4 cut(s)	173, 877, 1319, 1399
Mly113I	GGCGCC	1 cut(s)	1490
MmeI	TCCRAC	4 cut(s)	202, 567, 1637, 1853
MseI	TTAA	4 cut(s)	474, 507, 1842, 1912
MslI	CAYNNNNRTG	2 cut(s)	872, 1742
MspA1I	CMGCKG	1 cut(s)	1214
MspR9I	CCNGG	7 cut(s)	126, 871, 887, 977, 1015, 1358, 1625
MvaI	CCWGG	1 cut(s)	887
NarI	GGCGCC	1 cut(s)	1490
NciI	CCSGG	6 cut(s)	126, 871, 977, 1015, 1358, 1625
NcoI	CCATGG	2 cut(s)	1443, 1767
NdeII	GATC	8 cut(s)	50, 113, 156, 861, 1224, 1389, 1596, 1693
NlaIV	GGNNCC	9 cut(s)	388, 519, 981, 1310, 1491, 1582, 1611, 1733, 1773
NmeAIII	GCCGAG	2 cut(s)	1638, 1808
NmuCI	GTSAC	2 cut(s)	804, 1501
NruI	TCGCGA	1 cut(s)	893
NspI	RCATGY	1 cut(s)	1426
PaeI	GCATGC	1 cut(s)	1426
PauI	GCGCGC	1 cut(s)	1550
PfeI	GAWTC	6 cut(s)	274, 432, 685, 895, 1361, 1798
PflFI	GACNNNGTC	2 cut(s)	1024, 1786
Ple19I	CGATCG	2 cut(s)	1392, 1599
PluTI	GGCGCC	1 cut(s)	1493
PmaCI	CACGTG	1 cut(s)	792
PmlI	CACGTG	1 cut(s)	792
Ppu21I	YACGTR	2 cut(s)	792, 1168
PshAI	GACNNNNGTC	3 cut(s)	887, 1508, 1807
Psp124BI	GAGCTC	2 cut(s)	1467, 1524
Psp6I	CCWGG	1 cut(s)	885
PspCI	CACGTG	1 cut(s)	792
PspFI	CCCAGC	5 cut(s)	117, 196, 243, 1296, 1366
PspGI	CCWGG	1 cut(s)	885
PspN4I	GGNNCC	9 cut(s)	388, 519, 981, 1310, 1491, 1582, 1611, 1733, 1773
PspOMI	GGGCCC	1 cut(s)	1731
PspPI	GGNCC	9 cut(s)	669, 1105, 1209, 1270, 1309, 1731, 1732, 1764, 1772
PstNI	CAGNNNCTG	2 cut(s)	688, 1282
PsuI	RGATCY	1 cut(s)	861
PsyI	GACNNNGTC	2 cut(s)	1024, 1786
PteI	GCGCGC	1 cut(s)	1550
PvuI	CGATCG	2 cut(s)	1392, 1599
RruI	TCGCGA	1 cut(s)	893
RsaI	GTAC	4 cut(s)	697, 1166, 1418, 1574
RsaNI	GTAC	4 cut(s)	696, 1165, 1417, 1573
RseI	CAYNNNNRTG	2 cut(s)	872, 1742
SacI	GAGCTC	2 cut(s)	1467, 1524
SalI	GTCGAC	1 cut(s)	1018
SaqAI	TTAA	4 cut(s)	474, 507, 1842, 1912
Sau3AI	GATC	8 cut(s)	50, 113, 156, 861, 1224, 1389, 1596, 1693
Sau96I	GGNCC	9 cut(s)	669, 1105, 1209, 1270, 1309, 1731, 1732, 1764, 1772
ScrFI	CCNGG	7 cut(s)	126, 871, 887, 977, 1015, 1358, 1625
SduI	GDGCHC	4 cut(s)	1467, 1524, 1585, 1735
SfaNI	GCATC	2 cut(s)	158, 1907
SfoI	GGCGCC	1 cut(s)	1491
SinI	GGWCC	5 cut(s)	669, 1105, 1270, 1309, 1772
SmiMI	CAYNNNNRTG	2 cut(s)	872, 1742
SmlI	CTYRAG	1 cut(s)	305
SmoI	CTYRAG	1 cut(s)	305
SphI	GCATGC	1 cut(s)	1426
Sse9I	AATT	4 cut(s)	173, 877, 1319, 1399
SspDI	GGCGCC	1 cut(s)	1489
SspMI	CTAG	2 cut(s)	900, 1088
SstI	GAGCTC	2 cut(s)	1467, 1524
StyD4I	CCNGG	7 cut(s)	124, 869, 885, 975, 1013, 1356, 1623
StyI	CCWWGG	2 cut(s)	1443, 1767
TaaI	ACNGT	5 cut(s)	1234, 1416, 1609, 1840, 1894
TaiI	ACGT	3 cut(s)	794, 1170, 1329
TaqII	GACCGA	1 cut(s)	1799
TasI	AATT	4 cut(s)	173, 877, 1319, 1399
TatI	WGTACW	1 cut(s)	1416
TauI	GCSGC	7 cut(s)	68, 821, 854, 1073, 1474, 1495, 1884
TfiI	GAWTC	6 cut(s)	274, 432, 685, 895, 1361, 1798
Tru1I	TTAA	4 cut(s)	474, 507, 1842, 1912
Tru9I	TTAA	4 cut(s)	474, 507, 1842, 1912
TseFI	GTSAC	2 cut(s)	804, 1501
TseI	GCWGC	7 cut(s)	68, 200, 247, 454, 577, 701, 1566
Tsp45I	GTSAC	2 cut(s)	804, 1501
TspDTI	ATGAA	5 cut(s)	89, 890, 922, 1118, 1534
TspGWI	ACGGA	5 cut(s)	757, 1035, 1118, 1301, 1593
Tth111I	GACNNNGTC	2 cut(s)	1024, 1786
VpaK11BI	GGWCC	5 cut(s)	669, 1105, 1270, 1309, 1772
XapI	RAATTY	3 cut(s)	877, 1319, 1399
XceI	RCATGY	1 cut(s)	1426
XmiI	GTMKAC	1 cut(s)	1019
XspI	CTAG	2 cut(s)	900, 1088

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.