pycom01g21830

Protein of unknown function (DUF819)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009936
Physical Location & Seq
Reverse (-)
19678927 .. 19681204
2278 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1362 bp
ATGTCCATGGCATTGAAACTCACCGTTTCGCCCGCCTCTCGTCCGCCGTTGCCGACGCTCCAACTGTCACATTTCCCTTCCCGCCAAAACTTGAGCCTGAGTGTGCGTGCCTCCTCCCCGGGCGCCACCGTCATCGGCACAAATCAGAGCTTTCTCTCCCCTCCGAATCCTCCCAATTGGGACCGATCTGTGACTGCCAAGGCCGGTTTCCAGCTCAATGTGAACGCCCCTCTCATCTCTGCTCATGACCAATGGGGCATCTGGACGGTTCTCTTCGCCACCGGCGCTTTCGGTATCTGGTCAGAGAAGAACACGAAGATTGGGAGCGCATTGAGTGGGGCGTTGATGAGCATATTGATCGGACTTGCAGCCAGCAGTTTGGGAATCATTTCTTCGAATTCGCCCGCGTATTCGATTGTGTTGGAGTTCCTGCTCCCGCTGGCTGTTCCGTTGCTTCTGTACAGGGCGGATTTGCGCCGCGTGGTCAAATCAACCGGCGCACTTCTCTTGGCCTTCTTGGTTGGATCAGTTGCAACAACAGTTGGAACAGTGGTGGCGTATCTATTGGTGCCAATGCGATCGCTTGGTTCAAACAGTTGGAAAATAGCAGCTGCACTCATGGGCAGACATATCGGTGGAGCTGTCAATTATGTTGCCATATCTGATGCTCTTGGGGTCTCTCCGTCAATTTTAGCGGCCGGCCTAGCTGCAGATAATGTCATTTGTGCTGTATATTTTTCAACATTGTTTGCGTTGGCTTCTAGAGTACCTCCTGAGCCTCCTGTATCTGATGATGGCATTGCAAAGGGTGCAGACTCTGAGCCTGCAAACAAGCTTCCTCTGATACAAACTGCTACGTCCCTTGCCGTGTCCTTAGCCATCTGCAAGGCTGGACATTACGTCACGAAATATTATGGAATTCAAGGAGGTATCCTTCCAGCTGTGACAGCCATTGTTGTTATTCTGGCGACTCTGTTTCCCAGACAGTTTGCGTATCTCGCACCAACCGGTGAGGCTATGGCTATGATCCTAATGCAGGTATTTTTTACAGTCGTGGGAGCAAGCGGGAACATATGGAGTGTGATTAACACCGCTCCTAGTATCTTCTTATTTGCTTTAATCCAGGTCGCTGTTCATCTTGCTGTGATCCTCGGAATGGGAAAGCTATTGGGGTTTGACCTAAAGCAGTTGCTTATAGCGTCGAATGCCAATGTTGGAGGCCCTACAACAGCATGCGGAATGGCCACGGCCAAAGAGTGGCCGTCTATGATTGTTCCTGGTATTCTTGTCGGCATTTTTGGCATTGCGATCGCAACTTTCATCGGCATTGGATTTGGGGTTGCGGTTCTAAAATACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF819

Protein Analysis

454

Amino Acids

46.98

Weight (kDa)

9.58

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF819 PF05684 89 - 451 2e-122 Protein of unknown function (DUF819)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1027
AccB1I GGYRCC 2 cut(s) 122, 568
AccB7I CCANNNNNTGG 1 cut(s) 1257
AccBSI CCGCTC 1 cut(s) 1094
AccII CGCG 2 cut(s) 407, 480
AclWI GGATC 3 cut(s) 532, 1021, 1141
AcoI YGGCCR 4 cut(s) 696, 1242, 1248, 1259
AcsI RAATTY 2 cut(s) 397, 918
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 2 cut(s) 461, 768
AfiI CCNNNNNNNGG 3 cut(s) 1036, 1156, 1257
AflIII ACRYGT 1 cut(s) 1356
AgeI ACCGGT 1 cut(s) 1007
AgsI TTSAA 4 cut(s) 16, 591, 741, 923
AhdI GACNNNNNGTC 1 cut(s) 899
AjnI CCWGG 2 cut(s) 1122, 1276
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AluBI AGCT 8 cut(s) 150, 214, 611, 641, 707, 835, 941, 1165
AluI AGCT 8 cut(s) 150, 214, 611, 641, 707, 835, 941, 1165
Alw26I GTCTC 1 cut(s) 682
AlwI GGATC 3 cut(s) 532, 1021, 1141
AlwNI CAGNNNCTG 1 cut(s) 818
Ama87I CYCGRG 1 cut(s) 118
AoxI GGCC 8 cut(s) 201, 510, 696, 700, 1219, 1242, 1248, 1259
ApeKI GCWGC 4 cut(s) 368, 608, 611, 707
ApoI RAATTY 2 cut(s) 397, 918
AsiGI ACCGGT 1 cut(s) 1007
AsiSI GCGATCGC 2 cut(s) 581, 1311
Asp700I GAANNNNTTC 1 cut(s) 388
AspLEI GCGC 5 cut(s) 125, 287, 329, 477, 500
AspS9I GGNCC 2 cut(s) 181, 1220
AsuC2I CCSGG 2 cut(s) 119, 120
AsuHPI GGTGA 2 cut(s) 13, 1022
AsuII TTCGAA 1 cut(s) 395
AvaI CYCGRG 1 cut(s) 118
AvaII GGWCC 1 cut(s) 181
BalI TGGCCA 1 cut(s) 1244
BanI GGYRCC 2 cut(s) 122, 568
BbvI GCAGC 4 cut(s) 380, 598, 620, 694
BccI CCATC 2 cut(s) 788, 887
BceAI ACGGC 4 cut(s) 31, 851, 1246, 1263
BcgI CGANNNNNNTGC 6 cut(s) 340, 374, 613, 647, 1291, 1325
BciT130I CCWGG 2 cut(s) 1124, 1278
BciVI GTATCC 1 cut(s) 941
BcnI CCSGG 2 cut(s) 119, 120
BcoDI GTCTC 1 cut(s) 682
BfaI CTAG 3 cut(s) 704, 762, 1098
BfmI CTRYAG 1 cut(s) 708
BfoI RGCGCY 2 cut(s) 126, 288
BfuAI ACCTGC 1 cut(s) 1027
BfuI GTATCC 1 cut(s) 941
BisI GCNGC 6 cut(s) 369, 478, 609, 612, 696, 708
BlsI GCNGC 6 cut(s) 370, 479, 610, 613, 697, 709
Bme1390I CCNGG 4 cut(s) 119, 120, 1124, 1278
Bme18I GGWCC 1 cut(s) 181
BmeRI GACNNNNNGTC 1 cut(s) 899
BmeT110I CYCGRG 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 181, 1220
BmiI GGNNCC 3 cut(s) 124, 182, 570
BmrFI CCNGG 4 cut(s) 119, 120, 1124, 1278
BmsI GCATC 2 cut(s) 267, 655
Bpu10I CCTNAGC 2 cut(s) 774, 874
Bpu14I TTCGAA 1 cut(s) 395
BpuEI CTTGAG 1 cut(s) 112
BpuMI CCSGG 2 cut(s) 119, 120
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 682
BsaJI CCNNGG 6 cut(s) 6, 117, 118, 198, 1150, 1245
BsaWI WCCGGW 1 cut(s) 1007
Bsc4I CCNNNNNNNGG 3 cut(s) 1036, 1156, 1257
Bse118I RCCGGY 5 cut(s) 203, 281, 494, 698, 1007
Bse3DI GCAATG 2 cut(s) 798, 1302
BseBI CCWGG 2 cut(s) 1124, 1278
BseDI CCNNGG 6 cut(s) 6, 117, 118, 198, 1150, 1245
BseLI CCNNNNNNNGG 3 cut(s) 1036, 1156, 1257
BseMI GCAATG 2 cut(s) 798, 1302
BseMII CTCAG 3 cut(s) 89, 765, 810
BseRI GAGGAG 1 cut(s) 103
BseX3I CGGCCG 1 cut(s) 696
BseXI GCAGC 4 cut(s) 380, 598, 620, 694
BsgI GTGCAG 2 cut(s) 597, 831
Bsh1236I CGCG 2 cut(s) 407, 480
Bsh1285I CGRYCG 3 cut(s) 581, 699, 1311
BshFI GGCC 8 cut(s) 203, 512, 698, 702, 1221, 1244, 1250, 1261
BshNI GGYRCC 2 cut(s) 122, 568
BshTI ACCGGT 1 cut(s) 1007
BsiEI CGRYCG 3 cut(s) 581, 699, 1311
BsiHKCI CYCGRG 1 cut(s) 118
BsiSI CCGG 6 cut(s) 119, 204, 282, 495, 699, 1008
BslFI GGGAC 2 cut(s) 194, 844
BslI CCNNNNNNNGG 3 cut(s) 1036, 1156, 1257
BsmAI GTCTC 1 cut(s) 682
BsmFI GGGAC 2 cut(s) 194, 844
BsmI GAATGC 1 cut(s) 1210
BsnI GGCC 8 cut(s) 203, 512, 698, 702, 1221, 1244, 1250, 1261
Bso31I GGTCTC 1 cut(s) 682
BsoBI CYCGRG 1 cut(s) 118
Bsp119I TTCGAA 1 cut(s) 395
Bsp1407I TGTACA 1 cut(s) 459
Bsp143I GATC 7 cut(s) 185, 357, 524, 578, 1026, 1146, 1308
Bsp19I CCATGG 1 cut(s) 6
BspANI GGCC 8 cut(s) 203, 512, 698, 702, 1221, 1244, 1250, 1261
BspCNI CTCAG 3 cut(s) 90, 766, 811
BspFNI CGCG 2 cut(s) 407, 480
BspHI TCATGA 1 cut(s) 244
BspLI GGNNCC 3 cut(s) 124, 182, 570
BspMAI CTGCAG 1 cut(s) 712
BspMI ACCTGC 1 cut(s) 1027
BspPI GGATC 3 cut(s) 532, 1021, 1141
BspT104I TTCGAA 1 cut(s) 395
BspT107I GGYRCC 2 cut(s) 122, 568
BspTNI GGTCTC 1 cut(s) 682
BsrBI CCGCTC 1 cut(s) 1094
BsrDI GCAATG 2 cut(s) 798, 1302
BsrFI RCCGGY 5 cut(s) 203, 281, 494, 698, 1007
BsrGI TGTACA 1 cut(s) 459
BssAI RCCGGY 5 cut(s) 203, 281, 494, 698, 1007
BssECI CCNNGG 6 cut(s) 6, 117, 118, 198, 1150, 1245
BssMI GATC 7 cut(s) 185, 357, 524, 578, 1026, 1146, 1308
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 2 cut(s) 6, 198
Bst2UI CCWGG 2 cut(s) 1124, 1278
Bst4CI ACNGT 9 cut(s) 25, 66, 130, 268, 541, 550, 596, 987, 1051
Bst6I CTCTTC 1 cut(s) 278
BstACI GRCGYC 1 cut(s) 123
BstAPI GCANNNNNTGC 1 cut(s) 809
BstAUI TGTACA 1 cut(s) 459
BstBI TTCGAA 1 cut(s) 395
BstC8I GCNNGC 9 cut(s) 33, 108, 373, 405, 441, 700, 825, 1063, 1234
BstDEI CTNAG 4 cut(s) 98, 774, 819, 874
BstDSI CCRYGG 2 cut(s) 6, 1245
BstENI CCTNNNNNAGG 1 cut(s) 1034
BstFNI CGCG 2 cut(s) 407, 480
BstH2I RGCGCY 2 cut(s) 126, 288
BstHHI GCGC 5 cut(s) 125, 287, 329, 477, 500
BstKTI GATC 7 cut(s) 188, 360, 527, 581, 1029, 1149, 1311
BstMAI GTCTC 1 cut(s) 682
BstMBI GATC 7 cut(s) 185, 357, 524, 578, 1026, 1146, 1308
BstMCI CGRYCG 3 cut(s) 581, 699, 1311
BstMWI GCNNNNNNNGC 7 cut(s) 284, 704, 809, 947, 998, 1205, 1299
BstNI CCWGG 2 cut(s) 1124, 1278
BstNSI RCATGY 2 cut(s) 1236, 1360
BstSCI CCNGG 4 cut(s) 117, 118, 1122, 1276
BstSFI CTRYAG 1 cut(s) 708
BstUI CGCG 2 cut(s) 407, 480
BstV1I GCAGC 4 cut(s) 380, 598, 620, 694
BstXI CCANNNNNNTGG 1 cut(s) 379
BstZI CGGCCG 1 cut(s) 696
BsuI GTATCC 1 cut(s) 941
BsuRI GGCC 8 cut(s) 203, 512, 698, 702, 1221, 1244, 1250, 1261
BtgI CCRYGG 2 cut(s) 6, 1245
BtsIMutI CAGTG 1 cut(s) 555
BveI ACCTGC 1 cut(s) 1027
Cac8I GCNNGC 9 cut(s) 33, 108, 373, 405, 441, 700, 825, 1063, 1234
CaiI CAGNNNCTG 1 cut(s) 818
CciI TCATGA 1 cut(s) 244
CfoI GCGC 5 cut(s) 125, 287, 329, 477, 500
Cfr10I RCCGGY 5 cut(s) 203, 281, 494, 698, 1007
Cfr13I GGNCC 2 cut(s) 181, 1220
Cfr9I CCCGGG 1 cut(s) 118
CseI GACGC 2 cut(s) 64, 1188
Csp6I GTAC 2 cut(s) 460, 767
CspAI ACCGGT 1 cut(s) 1007
CviAII CATG 5 cut(s) 7, 245, 619, 1233, 1357
CviQI GTAC 2 cut(s) 460, 767
DdeI CTNAG 4 cut(s) 98, 774, 819, 874
DinI GGCGCC 1 cut(s) 124
DpnI GATC 7 cut(s) 187, 359, 526, 580, 1028, 1148, 1310
DpnII GATC 7 cut(s) 185, 357, 524, 578, 1026, 1146, 1308
DriI GACNNNNNGTC 1 cut(s) 899
EaeI YGGCCR 4 cut(s) 696, 1242, 1248, 1259
EagI CGGCCG 1 cut(s) 696
Eam1104I CTCTTC 1 cut(s) 278
Eam1105I GACNNNNNGTC 1 cut(s) 899
EarI CTCTTC 1 cut(s) 278
EciI GGCGGA 2 cut(s) 33, 482
EclXI CGGCCG 1 cut(s) 696
Eco130I CCWWGG 2 cut(s) 6, 198
Eco31I GGTCTC 1 cut(s) 682
Eco47I GGWCC 1 cut(s) 181
Eco52I CGGCCG 1 cut(s) 696
Eco88I CYCGRG 1 cut(s) 118
EcoNI CCTNNNNNAGG 1 cut(s) 1034
EcoO109I RGGNCCY 1 cut(s) 1220
EcoRI GAATTC 2 cut(s) 397, 918
EcoRII CCWGG 2 cut(s) 1122, 1276
EcoT14I CCWWGG 2 cut(s) 6, 198
EgeI GGCGCC 1 cut(s) 124
EheI GGCGCC 1 cut(s) 124
ErhI CCWWGG 2 cut(s) 6, 198
FaeI CATG 5 cut(s) 10, 248, 622, 1236, 1360
FaqI GGGAC 2 cut(s) 194, 844
FatI CATG 5 cut(s) 6, 244, 618, 1232, 1356
FauI CCCGC 5 cut(s) 40, 89, 412, 444, 1058
FauNDI CATATG 1 cut(s) 1073
Fnu4HI GCNGC 6 cut(s) 369, 478, 609, 612, 696, 708
FseI GGCCGGCC 1 cut(s) 702
Fsp4HI GCNGC 6 cut(s) 369, 478, 609, 612, 696, 708
FspBI CTAG 3 cut(s) 704, 762, 1098
GlaI GCGC 5 cut(s) 124, 286, 328, 476, 499
GluI GCNGC 6 cut(s) 369, 478, 609, 612, 696, 708
HaeII RGCGCY 2 cut(s) 126, 288
HaeIII GGCC 8 cut(s) 203, 512, 698, 702, 1221, 1244, 1250, 1261
HapII CCGG 6 cut(s) 119, 204, 282, 495, 699, 1008
HgaI GACGC 2 cut(s) 64, 1188
HhaI GCGC 5 cut(s) 125, 287, 329, 477, 500
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 5 cut(s) 10, 248, 622, 1236, 1360
Hin6I GCGC 5 cut(s) 123, 285, 327, 475, 498
HinP1I GCGC 5 cut(s) 123, 285, 327, 475, 498
HindIII AAGCTT 1 cut(s) 833
HinfI GANTC 4 cut(s) 166, 384, 815, 970
HpaII CCGG 6 cut(s) 119, 204, 282, 495, 699, 1008
HphI GGTGA 2 cut(s) 13, 1022
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 9 cut(s) 147, 165, 304, 362, 664, 790, 820, 843, 1154
Hpy188III TCNNGA 5 cut(s) 245, 262, 762, 773, 904
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 2 cut(s) 58, 1204
HpyAV CCTTC 3 cut(s) 87, 523, 944
HpyCH4III ACNGT 9 cut(s) 25, 66, 130, 268, 541, 550, 596, 987, 1051
HpyCH4IV ACGT 2 cut(s) 857, 900
HpyCH4V TGCA 9 cut(s) 368, 533, 614, 710, 803, 812, 827, 885, 1036
HpyF10VI GCNNNNNNNGC 7 cut(s) 284, 704, 809, 947, 998, 1205, 1299
HpyF3I CTNAG 4 cut(s) 98, 774, 819, 874
HpySE526I ACGT 2 cut(s) 857, 900
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 5 cut(s) 10, 248, 622, 1236, 1360
HspAI GCGC 5 cut(s) 123, 285, 327, 475, 498
KasI GGCGCC 1 cut(s) 122
KroI GCCGGC 1 cut(s) 698
KroNI GCCGGC 1 cut(s) 700
Kzo9I GATC 7 cut(s) 185, 357, 524, 578, 1026, 1146, 1308
LmnI GCTCC 6 cut(s) 63, 324, 438, 638, 1058, 1099
Lsp1109I GCAGC 4 cut(s) 380, 598, 620, 694
LweI GCATC 2 cut(s) 267, 655
MaeI CTAG 3 cut(s) 704, 762, 1098
MaeII ACGT 2 cut(s) 857, 900
MaeIII GTNAC 4 cut(s) 66, 190, 901, 943
MalI GATC 7 cut(s) 187, 359, 526, 580, 1028, 1148, 1310
MbiI CCGCTC 1 cut(s) 1094
MboI GATC 7 cut(s) 185, 357, 524, 578, 1026, 1146, 1308
MboII GAAGA 5 cut(s) 265, 319, 328, 384, 1096
MfeI CAATTG 1 cut(s) 175
MlsI TGGCCA 1 cut(s) 1244
MluCI AATT 5 cut(s) 175, 397, 646, 687, 918
MluNI TGGCCA 1 cut(s) 1244
Mly113I GGCGCC 1 cut(s) 123
MlyI GAGTC 2 cut(s) 809, 964
MmeI TCCRAC 6 cut(s) 85, 402, 502, 523, 578, 1195
Mox20I TGGCCA 1 cut(s) 1244
MroNI GCCGGC 1 cut(s) 698
MroXI GAANNNNTTC 1 cut(s) 388
MscI TGGCCA 1 cut(s) 1244
MseI TTAA 2 cut(s) 1086, 1118
MslI CAYNNNNRTG 1 cut(s) 633
Msp20I TGGCCA 1 cut(s) 1244
MspA1I CMGCKG 3 cut(s) 439, 611, 941
MspI CCGG 6 cut(s) 119, 204, 282, 495, 699, 1008
MspR9I CCNGG 4 cut(s) 119, 120, 1124, 1278
MunI CAATTG 1 cut(s) 175
Mva1269I GAATGC 1 cut(s) 1210
MvaI CCWGG 2 cut(s) 1124, 1278
MvnI CGCG 2 cut(s) 407, 480
MwoI GCNNNNNNNGC 7 cut(s) 284, 704, 809, 947, 998, 1205, 1299
NaeI GCCGGC 1 cut(s) 700
NarI GGCGCC 1 cut(s) 123
NciI CCSGG 2 cut(s) 119, 120
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 1073
NdeII GATC 7 cut(s) 185, 357, 524, 578, 1026, 1146, 1308
NgoMIV GCCGGC 1 cut(s) 698
NlaIII CATG 5 cut(s) 10, 248, 622, 1236, 1360
NlaIV GGNNCC 3 cut(s) 124, 182, 570
NmuCI GTSAC 4 cut(s) 66, 190, 901, 943
NspI RCATGY 2 cut(s) 1236, 1360
NspV TTCGAA 1 cut(s) 395
PaeI GCATGC 1 cut(s) 1236
PagI TCATGA 1 cut(s) 244
PciI ACATGT 1 cut(s) 1356
PctI GAATGC 1 cut(s) 1210
PdiI GCCGGC 1 cut(s) 700
PdmI GAANNNNTTC 1 cut(s) 388
PfeI GAWTC 2 cut(s) 166, 384
PflMI CCANNNNNTGG 1 cut(s) 1257
PinAI ACCGGT 1 cut(s) 1007
PkrI GCNGC 6 cut(s) 370, 479, 610, 613, 697, 709
Ple19I CGATCG 2 cut(s) 581, 1311
PleI GAGTC 2 cut(s) 809, 964
PluTI GGCGCC 1 cut(s) 126
PpsI GAGTC 2 cut(s) 809, 964
PscI ACATGT 1 cut(s) 1356
Psp6I CCWGG 2 cut(s) 1122, 1276
PspGI CCWGG 2 cut(s) 1122, 1276
PspN4I GGNNCC 3 cut(s) 124, 182, 570
PspPI GGNCC 2 cut(s) 181, 1220
PstI CTGCAG 1 cut(s) 712
PstNI CAGNNNCTG 1 cut(s) 818
PvuI CGATCG 2 cut(s) 581, 1311
PvuII CAGCTG 2 cut(s) 611, 941
RgaI GCGATCGC 2 cut(s) 581, 1311
RigI GGCCGGCC 1 cut(s) 702
RsaI GTAC 2 cut(s) 461, 768
RsaNI GTAC 2 cut(s) 460, 767
RseI CAYNNNNRTG 1 cut(s) 633
SaqAI TTAA 2 cut(s) 1086, 1118
SatI GCNGC 6 cut(s) 369, 478, 609, 612, 696, 708
Sau3AI GATC 7 cut(s) 185, 357, 524, 578, 1026, 1146, 1308
Sau96I GGNCC 2 cut(s) 181, 1220
SchI GAGTC 2 cut(s) 809, 964
ScrFI CCNGG 4 cut(s) 119, 120, 1124, 1278
SfaAI GCGATCGC 2 cut(s) 581, 1311
SfaNI GCATC 2 cut(s) 267, 655
SfcI CTRYAG 1 cut(s) 708
SfoI GGCGCC 1 cut(s) 124
SfuI TTCGAA 1 cut(s) 395
SgfI GCGATCGC 2 cut(s) 581, 1311
SgrAI CRCCGGYG 1 cut(s) 281
SinI GGWCC 1 cut(s) 181
SmaI CCCGGG 1 cut(s) 120
SmiMI CAYNNNNRTG 1 cut(s) 633
SmlI CTYRAG 1 cut(s) 91
SmoI CTYRAG 1 cut(s) 91
SphI GCATGC 1 cut(s) 1236
Sse9I AATT 5 cut(s) 175, 397, 646, 687, 918
SspDI GGCGCC 1 cut(s) 122
SspI AATATT 1 cut(s) 911
SspMI CTAG 3 cut(s) 704, 762, 1098
StyD4I CCNGG 4 cut(s) 117, 118, 1122, 1276
StyI CCWWGG 2 cut(s) 6, 198
TaaI ACNGT 9 cut(s) 25, 66, 130, 268, 541, 550, 596, 987, 1051
TaiI ACGT 2 cut(s) 860, 903
TaqI TCGA 3 cut(s) 395, 413, 1202
TaqII GACCGA 1 cut(s) 198
TasI AATT 5 cut(s) 175, 397, 646, 687, 918
TatI WGTACW 1 cut(s) 459
TauI GCSGC 2 cut(s) 480, 698
TfiI GAWTC 2 cut(s) 166, 384
Tru1I TTAA 2 cut(s) 1086, 1118
Tru9I TTAA 2 cut(s) 1086, 1118
TscAI CASTG 1 cut(s) 555
TseFI GTSAC 4 cut(s) 66, 190, 901, 943
TseI GCWGC 4 cut(s) 368, 608, 611, 707
Tsp45I GTSAC 4 cut(s) 66, 190, 901, 943
TspDTI ATGAA 2 cut(s) 1124, 1309
TspGWI ACGGA 2 cut(s) 438, 672
TspMI CCCGGG 1 cut(s) 118
TspRI CASTG 1 cut(s) 555
Van91I CCANNNNNTGG 1 cut(s) 1257
VpaK11BI GGWCC 1 cut(s) 181
XagI CCTNNNNNAGG 1 cut(s) 1034
XapI RAATTY 2 cut(s) 397, 918
XbaI TCTAGA 1 cut(s) 761
XceI RCATGY 2 cut(s) 1236, 1360
XmaI CCCGGG 1 cut(s) 118
XmnI GAANNNNTTC 1 cut(s) 388
XspI CTAG 3 cut(s) 704, 762, 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.