pycom01g23300

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009257
Physical Location & Seq
Reverse (-)
20657253 .. 20660182
2930 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2178 bp
ATGGGTAGCTTTAGCGGAGAGGAAGAAGATTGTGGATTCTTTGACGCAGAAGAGGAAATTGCATCGACATCCGATGCAGGGTCTGAAACCATTGAGACATTCGATTCCAATTCTTCCTTCATCAATTGGGTTCCGGTTGGTTTTCCCTATGATTTGTGGAATAGAACCCCGGGAAGTGTTAAGGACCGGCGGGCCAAGTTTCTGGAGTGGATGGGACTGAGCGTGGATCAAAGTTTGGGGGAGAATTCTGTGGAAATAACGAGTGATGTAGCAGGGGTGGTTGAGAGAGTTGGAGAGAGTAGTGGGGCTGTGTTGAGAACCACCTCATGTTTTGAGGATGAGTTTTGTTTGAGTCGGTCATTTGTGTCATGTCGGTGTAATGACCATTCCAATACCTCAATTGAGTTGGATTGGAAGGATGATTCTGCATGTAGAGATGGGAATTTAGGGAAGCCTTTGGAGTGTAATGCAGATGAAGTGAGGCTGGATCCGAATGTACGTGAAGGTCAAGAAGTGACCGAAGAGTCTAAGCGCACTCCGTGTTCTTCATCTTCATTGTCTCAACAATTGATGGAGAATCGAATGGAGGAGGCGAACGCTAGAGCTGGGATACTGAAGAGAGTTAAGAAGGGGTGGTTGAGTAGGTTGCGGTCAATCACATGCCTTGTTGATACGCATGGGGAGGATGATGAATTGGCACACAACGGCAATGCTGCAATCATGGGATTCAGGGCACAAAGAGTTAAGGTTCACCAGTGTAGGAAGCGGTTGAAGGAACTTTCGGCTCTTCACATGGGGCAAGATATCCAGGCACATGAGGGCGCAATTTTGGCAATGAAATTCAGTCCCGATGGACAATACTTAGCGAGTGGTGGCGAAGATGGGATTGTGCGAGTCTGGCAAGTAGTGGAGAATGAGAGATGTAATGAACATGACATTCCGAAAATTGATCCATCCTGCATTTACTTCACAGTGAATCATCTCTCTGAACTAAATCCACTATTTTCGGAGAAGGATAAAATAGGCAAATCAAGTAGCCTTAGGAAAACATCGGATTCAGCATGTGTTATTTTTCCTCCTAAAGTCTTCCGGATATTGGAGAAACCATTGCATGAGTTTCATGGGCATGGTGATGATATATTGGATCTCTCTTGGTCAAGTAGTAATTATCTTCTGTCATCTTCTATTGACAAAACTGTTCGTCTTTGGCAAGTTGGATGCGGCAGTTGCCTCAAAGTCTTTTCCCATAGTAATTATGTGACGAGTGTTCAATTTAATCCAGTGGATGAGAATTACTTCATCAGTGGTTCAATAGATGGCAAAGTACGTATTTGGGGAATTTCTTGCTGCCAAGTTGTTGATTGGAGTGACATAAGAGATATAGTTACTGTGGTGTGCTATTACCCTGATGGGCAGGGAGGAATTGTTGGCTCAATGACAGGCAGTTGCCGCTTTTATAATATATCAGATAATCTTTTGCAATTGGATGCTCAATTATGCTTGCAAAGTAAAAAGAAGTCTCCGTGCAGAAGGATTACAGGCTTCCAGTTTTTCCCACAAGATTCTAGCAAGGTGATGGTCACTACGGCCGATTCACGAGTCAGAATTCTTCATGGTCTTACTGTGGTCGGCAAGTATGCCGGACAACGAAATGCAGGGAACCAGACATCTGCTACCTTCACTTCAGATGGGAAACATATAGTCTCGGTTAGTGAGGATTCTAGTGTATATGTCTGGAACTGCAATAATCATGAAGAACGAGTTCTCTCTCAGGCAAAAAAGATCAGATCTTGTGAGCGCTTTTCCACGAATGTTTCTGTGGCGATACCTTGGTCTGGATTTAAATGTGGGACCATAGAAAATGAGAGGCAATTTCAGGCTGCTGATGAGAATTTACAGGAGGCTCTACCTTTTTCCTCACCTGCATGTTTTTCTCTCAGCCAAGATTCTTTCCTTGATTCTATCCCGAAGGGATCTGCAACTTGGCCAGAGGAGAAGCTTCCAACTTCGAGCCCACTTGCAAACCAATCTACTATGCTCATATCCGAGTACAAGTTTTTTAAAACTTCTTGCCAGAACACATCCAGTTCTCATGCATGGGGTATGGTTATTGTTACTGCAGGATGGGATGGATCGATCAGATCGTTCCACAATTATGGTTTACCAGTACCCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

726

Amino Acids

80.41

Weight (kDa)

5.32

Isoelectric Point (pI)

47.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 231 - 308 3.1e-09 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 235 - 304 5.2e-09 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 241 - 306 1.7e-06 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 256 - 447 3e-15 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 268 - 313 4.5e-07 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 269 - 305 3.4e-06 WDR5 beta-propeller domain
WD40 PF00400 269 - 300 3.7e-09 WD domain, G-beta repeat
WD40_Prp19 PF24814 367 - 491 2.9e-12 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 367 - 447 4.5e-12 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 368 - 585 1.1e-33 WDR5 beta-propeller domain
WD40 PF00400 368 - 403 3.1e-07 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 370 - 430 1.5e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 370 - 416 6.8e-06 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 371 - 471 9.6e-09 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 371 - 580 8e-10 G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1 PF24105 373 - 450 2.9e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 376 - 591 3.7e-21 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 389 - 493 3.4e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 408 - 445 3.9e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 430 - 613 3.4e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 451 - 585 4.2e-06 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 455 - 586 7.7e-06 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1458
AarI CACCTGC 1 cut(s) 1930
AasI GACNNNNNNGTC 1 cut(s) 523
Acc36I ACCTGC 1 cut(s) 1930
AccIII TCCGGA 1 cut(s) 1089
AciI CCGC 7 cut(s) 15, 190, 649, 766, 1221, 1450, 2172
AclWI GGATC 7 cut(s) 234, 482, 495, 944, 1152, 1981, 2140
AcoI YGGCCR 2 cut(s) 1587, 1985
AcsI RAATTY 6 cut(s) 244, 442, 839, 1338, 1605, 1891
AcuI CTGAAG 2 cut(s) 635, 1668
AdeI CACNNNGTG 1 cut(s) 540
AfaI GTAC 4 cut(s) 498, 1326, 2051, 2169
AfeI AGCGCT 1 cut(s) 1799
AfiI CCNNNNNNNGG 3 cut(s) 78, 1096, 1835
AgsI TTSAA 3 cut(s) 772, 1271, 1311
AjnI CCWGG 1 cut(s) 807
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AluBI AGCT 3 cut(s) 9, 605, 1999
AluI AGCT 3 cut(s) 9, 605, 1999
Alw26I GTCTC 4 cut(s) 89, 564, 1524, 1708
AlwI GGATC 7 cut(s) 234, 482, 495, 944, 1152, 1981, 2140
AlwNI CAGNNNCTG 1 cut(s) 83
Ama87I CYCGRG 1 cut(s) 169
Aor13HI TCCGGA 1 cut(s) 1089
Aor51HI AGCGCT 1 cut(s) 1799
AoxI GGCC 3 cut(s) 192, 1587, 1985
ApeKI GCWGC 3 cut(s) 713, 1347, 1880
ApoI RAATTY 6 cut(s) 244, 442, 839, 1338, 1605, 1891
Asp700I GAANNNNTTC 2 cut(s) 1295, 1761
AspLEI GCGC 3 cut(s) 534, 824, 1800
AspS9I GGNCC 3 cut(s) 184, 192, 1851
AsuC2I CCSGG 2 cut(s) 170, 171
AsuHPI GGTGA 4 cut(s) 743, 1142, 1585, 1911
AvaI CYCGRG 1 cut(s) 169
AvaII GGWCC 2 cut(s) 184, 1851
AxyI CCTNAGG 1 cut(s) 1040
BaeGI GKGCMC 1 cut(s) 736
BaeI ACNNNNGTAYC 2 cut(s) 1817, 1850
BalI TGGCCA 1 cut(s) 1987
BamHI GGATCC 1 cut(s) 487
BanII GRGCYC 1 cut(s) 2015
BauI CACGAG 1 cut(s) 1596
BbsI GAAGAC 1 cut(s) 1078
BbvI GCAGC 3 cut(s) 700, 1334, 1867
BceAI ACGGC 2 cut(s) 721, 1602
BciT130I CCWGG 1 cut(s) 809
BciVI GTATCC 1 cut(s) 603
BcnI CCSGG 2 cut(s) 170, 171
BcoDI GTCTC 4 cut(s) 89, 564, 1524, 1708
BfaI CTAG 3 cut(s) 600, 1566, 1722
BfmI CTRYAG 1 cut(s) 2118
BfoI RGCGCY 1 cut(s) 1801
BfuAI ACCTGC 1 cut(s) 1930
BfuI GTATCC 1 cut(s) 603
BglII AGATCT 1 cut(s) 1787
BisI GCNGC 5 cut(s) 714, 1222, 1348, 1450, 1881
BlsI GCNGC 5 cut(s) 715, 1223, 1349, 1451, 1882
Bme1390I CCNGG 3 cut(s) 170, 171, 809
Bme18I GGWCC 2 cut(s) 184, 1851
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 3 cut(s) 184, 192, 1851
BmiI GGNNCC 4 cut(s) 132, 489, 1661, 1852
BmrFI CCNGG 3 cut(s) 170, 171, 809
BmsI GCATC 4 cut(s) 64, 71, 1208, 1477
BpiI GAAGAC 1 cut(s) 1078
BpmI CTGGAG 1 cut(s) 224
BpuMI CCSGG 2 cut(s) 170, 171
Bsa29I ATCGAT 1 cut(s) 2135
BsaAI YACGTR 2 cut(s) 500, 1328
BsaJI CCNNGG 3 cut(s) 168, 169, 1829
BsaWI WCCGGW 2 cut(s) 133, 1089
BsaXI ACNNNNNCTCC 2 cut(s) 1410, 1440
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 1096, 1835
Bse118I RCCGGY 1 cut(s) 186
Bse1I ACTGG 5 cut(s) 754, 1280, 1546, 2085, 2165
Bse21I CCTNAGG 1 cut(s) 1040
Bse3DI GCAATG 3 cut(s) 715, 840, 1106
BseAI TCCGGA 1 cut(s) 1089
BseBI CCWGG 1 cut(s) 809
BseCI ATCGAT 1 cut(s) 2135
BseDI CCNNGG 3 cut(s) 168, 169, 1829
BseLI CCNNNNNNNGG 3 cut(s) 78, 1096, 1835
BseMI GCAATG 3 cut(s) 715, 840, 1106
BseMII CTCAG 3 cut(s) 209, 1784, 1951
BseNI ACTGG 5 cut(s) 754, 1280, 1546, 2085, 2165
BseRI GAGGAG 2 cut(s) 602, 2006
BseSI GKGCMC 1 cut(s) 736
BseX3I CGGCCG 1 cut(s) 1587
BseXI GCAGC 3 cut(s) 700, 1334, 1867
BseYI CCCAGC 1 cut(s) 605
BsgI GTGCAG 1 cut(s) 1546
Bsh1285I CGRYCG 1 cut(s) 1590
BshFI GGCC 3 cut(s) 194, 1589, 1987
BshVI ATCGAT 1 cut(s) 2135
BsiEI CGRYCG 1 cut(s) 1590
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 5 cut(s) 134, 170, 187, 1090, 1641
BslFI GGGAC 3 cut(s) 228, 831, 1864
BslI CCNNNNNNNGG 3 cut(s) 78, 1096, 1835
BsmAI GTCTC 4 cut(s) 89, 564, 1524, 1708
BsmFI GGGAC 3 cut(s) 228, 831, 1864
BsnI GGCC 3 cut(s) 194, 1589, 1987
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 2 cut(s) 736, 2015
Bsp13I TCCGGA 1 cut(s) 1089
BspACI CCGC 7 cut(s) 15, 190, 649, 766, 1221, 1450, 2172
BspANI GGCC 3 cut(s) 194, 1589, 1987
BspCNI CTCAG 3 cut(s) 210, 1783, 1950
BspDI ATCGAT 1 cut(s) 2135
BspEI TCCGGA 1 cut(s) 1089
BspHI TCATGA 1 cut(s) 1750
BspLI GGNNCC 4 cut(s) 132, 489, 1661, 1852
BspMAI CTGCAG 1 cut(s) 2122
BspMI ACCTGC 1 cut(s) 1930
BspPI GGATC 7 cut(s) 234, 482, 495, 944, 1152, 1981, 2140
BspQI GCTCTTC 1 cut(s) 792
BsrDI GCAATG 3 cut(s) 715, 840, 1106
BsrFI RCCGGY 1 cut(s) 186
BsrI ACTGG 5 cut(s) 754, 1280, 1546, 2085, 2165
BssAI RCCGGY 1 cut(s) 186
BssECI CCNNGG 3 cut(s) 168, 169, 1829
BssSI CACGAG 1 cut(s) 1596
BssT1I CCWWGG 1 cut(s) 1829
Bst2BI CACGAG 1 cut(s) 1596
Bst2UI CCWGG 1 cut(s) 809
Bst4CI ACNGT 4 cut(s) 973, 1198, 1390, 1624
Bst6I CTCTTC 4 cut(s) 45, 516, 611, 792
BstBAI YACGTR 2 cut(s) 500, 1328
BstC8I GCNNGC 2 cut(s) 192, 1502
BstDEI CTNAG 6 cut(s) 218, 528, 862, 1040, 1770, 1937
BstH2I RGCGCY 1 cut(s) 1801
BstHHI GCGC 3 cut(s) 534, 824, 1800
BstMAI GTCTC 4 cut(s) 89, 564, 1524, 1708
BstMCI CGRYCG 1 cut(s) 1590
BstMWI GCNNNNNNNGC 4 cut(s) 830, 898, 1227, 1449
BstNI CCWGG 1 cut(s) 809
BstNSI RCATGY 4 cut(s) 432, 663, 1065, 1929
BstSCI CCNGG 3 cut(s) 168, 169, 807
BstSFI CTRYAG 1 cut(s) 2118
BstSLI GKGCMC 1 cut(s) 736
BstSNI TACGTA 1 cut(s) 1328
BstV1I GCAGC 3 cut(s) 700, 1334, 1867
BstV2I GAAGAC 1 cut(s) 1078
BstX2I RGATCY 4 cut(s) 487, 1144, 1787, 1973
BstXI CCANNNNNNTGG 2 cut(s) 202, 2156
BstYI RGATCY 4 cut(s) 487, 1144, 1787, 1973
BstZI CGGCCG 1 cut(s) 1587
Bsu15I ATCGAT 1 cut(s) 2135
Bsu36I CCTNAGG 1 cut(s) 1040
BsuI GTATCC 1 cut(s) 603
BsuRI GGCC 3 cut(s) 194, 1589, 1987
BsuTUI ATCGAT 1 cut(s) 2135
BtsIMutI CAGTG 4 cut(s) 761, 978, 1287, 1309
BveI ACCTGC 1 cut(s) 1930
Cac8I GCNNGC 2 cut(s) 192, 1502
CaiI CAGNNNCTG 1 cut(s) 83
CciI TCATGA 1 cut(s) 1750
CfoI GCGC 3 cut(s) 534, 824, 1800
Cfr10I RCCGGY 1 cut(s) 186
Cfr13I GGNCC 3 cut(s) 184, 192, 1851
Cfr9I CCCGGG 1 cut(s) 169
ClaI ATCGAT 1 cut(s) 2135
CseI GACGC 1 cut(s) 53
Csp6I GTAC 4 cut(s) 497, 1325, 2050, 2168
CviQI GTAC 4 cut(s) 497, 1325, 2050, 2168
DdeI CTNAG 6 cut(s) 218, 528, 862, 1040, 1770, 1937
DraI TTTAAA 2 cut(s) 1843, 2062
DraIII CACNNNGTG 1 cut(s) 540
DrdI GACNNNNNNGTC 1 cut(s) 523
DseDI GACNNNNNNGTC 1 cut(s) 523
EaeI YGGCCR 2 cut(s) 1587, 1985
EagI CGGCCG 1 cut(s) 1587
Eam1104I CTCTTC 4 cut(s) 45, 516, 611, 792
EarI CTCTTC 4 cut(s) 45, 516, 611, 792
EclXI CGGCCG 1 cut(s) 1587
Eco105I TACGTA 1 cut(s) 1328
Eco130I CCWWGG 1 cut(s) 1829
Eco24I GRGCYC 1 cut(s) 2015
Eco32I GATATC 1 cut(s) 805
Eco47I GGWCC 2 cut(s) 184, 1851
Eco47III AGCGCT 1 cut(s) 1799
Eco52I CGGCCG 1 cut(s) 1587
Eco57I CTGAAG 2 cut(s) 635, 1668
Eco81I CCTNAGG 1 cut(s) 1040
Eco88I CYCGRG 1 cut(s) 169
EcoRI GAATTC 2 cut(s) 244, 1605
EcoRII CCWGG 1 cut(s) 807
EcoRV GATATC 1 cut(s) 805
EcoT14I CCWWGG 1 cut(s) 1829
EcoT22I ATGCAT 1 cut(s) 2098
EcoT38I GRGCYC 1 cut(s) 2015
ErhI CCWWGG 1 cut(s) 1829
FaqI GGGAC 3 cut(s) 228, 831, 1864
FauI CCCGC 1 cut(s) 183
Fnu4HI GCNGC 5 cut(s) 714, 1222, 1348, 1450, 1881
FriOI GRGCYC 1 cut(s) 2015
Fsp4HI GCNGC 5 cut(s) 714, 1222, 1348, 1450, 1881
FspBI CTAG 3 cut(s) 600, 1566, 1722
GlaI GCGC 3 cut(s) 533, 823, 1799
GluI GCNGC 5 cut(s) 714, 1222, 1348, 1450, 1881
GsaI CCCAGC 1 cut(s) 609
GsuI CTGGAG 1 cut(s) 224
HaeII RGCGCY 1 cut(s) 1801
HaeIII GGCC 3 cut(s) 194, 1589, 1987
HapII CCGG 5 cut(s) 134, 170, 187, 1090, 1641
HgaI GACGC 1 cut(s) 53
HhaI GCGC 3 cut(s) 534, 824, 1800
Hin6I GCGC 3 cut(s) 532, 822, 1798
HinP1I GCGC 3 cut(s) 532, 822, 1798
HindIII AAGCTT 1 cut(s) 1997
HpaII CCGG 5 cut(s) 134, 170, 187, 1090, 1641
HphI GGTGA 4 cut(s) 743, 1142, 1585, 1911
Hpy166II GTNNAC 2 cut(s) 751, 2162
Hpy188III TCNNGA 9 cut(s) 203, 509, 848, 1090, 1596, 1735, 1751, 1836, 1966
Hpy8I GTNNAC 2 cut(s) 751, 2162
HpyAV CCTTC 9 cut(s) 127, 409, 497, 622, 766, 1006, 1524, 1687, 1963
HpyCH4III ACNGT 4 cut(s) 973, 1198, 1390, 1624
HpyCH4IV ACGT 2 cut(s) 499, 1327
HpyF10VI GCNNNNNNNGC 4 cut(s) 830, 898, 1227, 1449
HpyF3I CTNAG 6 cut(s) 218, 528, 862, 1040, 1770, 1937
HpySE526I ACGT 2 cut(s) 499, 1327
HspAI GCGC 3 cut(s) 532, 822, 1798
Kpn2I TCCGGA 1 cut(s) 1089
LguI GCTCTTC 1 cut(s) 792
Lsp1109I GCAGC 3 cut(s) 700, 1334, 1867
LweI GCATC 4 cut(s) 64, 71, 1208, 1477
MaeI CTAG 3 cut(s) 600, 1566, 1722
MaeII ACGT 2 cut(s) 499, 1327
MaeIII GTNAC 6 cut(s) 514, 1258, 1367, 1384, 1579, 2113
MfeI CAATTG 4 cut(s) 124, 399, 566, 1481
MflI RGATCY 4 cut(s) 487, 1144, 1787, 1973
MhlI GDGCHC 2 cut(s) 736, 2015
MlsI TGGCCA 1 cut(s) 1987
MluNI TGGCCA 1 cut(s) 1987
MlyI GAGTC 4 cut(s) 361, 533, 903, 1608
MmeI TCCRAC 4 cut(s) 271, 387, 1195, 2027
Mox20I TGGCCA 1 cut(s) 1987
Mph1103I ATGCAT 1 cut(s) 2098
MroI TCCGGA 1 cut(s) 1089
MroXI GAANNNNTTC 2 cut(s) 1295, 1761
MscI TGGCCA 1 cut(s) 1987
MseI TTAA 6 cut(s) 180, 624, 744, 1275, 1842, 2061
MslI CAYNNNNRTG 5 cut(s) 373, 1125, 1131, 1924, 2154
Msp20I TGGCCA 1 cut(s) 1987
MspI CCGG 5 cut(s) 134, 170, 187, 1090, 1641
MspR9I CCNGG 3 cut(s) 170, 171, 809
MunI CAATTG 4 cut(s) 124, 399, 566, 1481
MvaI CCWGG 1 cut(s) 809
MwoI GCNNNNNNNGC 4 cut(s) 830, 898, 1227, 1449
NciI CCSGG 2 cut(s) 170, 171
NlaIV GGNNCC 4 cut(s) 132, 489, 1661, 1852
NmuCI GTSAC 4 cut(s) 514, 1258, 1367, 1579
NsiI ATGCAT 1 cut(s) 2098
NspI RCATGY 4 cut(s) 432, 663, 1065, 1929
PagI TCATGA 1 cut(s) 1750
PaqCI CACCTGC 1 cut(s) 1930
PciSI GCTCTTC 1 cut(s) 792
PcsI WCGNNNNNNNCGW 1 cut(s) 2141
PdmI GAANNNNTTC 2 cut(s) 1295, 1761
PkrI GCNGC 5 cut(s) 715, 1223, 1349, 1451, 1882
PleI GAGTC 4 cut(s) 360, 532, 902, 1607
PpsI GAGTC 4 cut(s) 360, 532, 902, 1607
Ppu21I YACGTR 2 cut(s) 500, 1328
PsiI TTATAA 1 cut(s) 1458
Psp6I CCWGG 1 cut(s) 807
PspFI CCCAGC 1 cut(s) 605
PspGI CCWGG 1 cut(s) 807
PspN4I GGNNCC 4 cut(s) 132, 489, 1661, 1852
PspPI GGNCC 3 cut(s) 184, 192, 1851
PstI CTGCAG 1 cut(s) 2122
PstNI CAGNNNCTG 1 cut(s) 83
PsuI RGATCY 4 cut(s) 487, 1144, 1787, 1973
RsaI GTAC 4 cut(s) 498, 1326, 2051, 2169
RsaNI GTAC 4 cut(s) 497, 1325, 2050, 2168
RseI CAYNNNNRTG 5 cut(s) 373, 1125, 1131, 1924, 2154
SapI GCTCTTC 1 cut(s) 792
SaqAI TTAA 6 cut(s) 180, 624, 744, 1275, 1842, 2061
SatI GCNGC 5 cut(s) 714, 1222, 1348, 1450, 1881
Sau96I GGNCC 3 cut(s) 184, 192, 1851
SchI GAGTC 4 cut(s) 361, 533, 903, 1608
ScrFI CCNGG 3 cut(s) 170, 171, 809
SduI GDGCHC 2 cut(s) 736, 2015
SfaNI GCATC 4 cut(s) 64, 71, 1208, 1477
SfcI CTRYAG 1 cut(s) 2118
SinI GGWCC 2 cut(s) 184, 1851
SmaI CCCGGG 1 cut(s) 171
SmiI ATTTAAAT 1 cut(s) 1843
SmiMI CAYNNNNRTG 5 cut(s) 373, 1125, 1131, 1924, 2154
SnaBI TACGTA 1 cut(s) 1328
SsiI CCGC 7 cut(s) 15, 190, 649, 766, 1221, 1450, 2172
SspMI CTAG 3 cut(s) 600, 1566, 1722
StyD4I CCNGG 3 cut(s) 168, 169, 807
StyI CCWWGG 1 cut(s) 1829
SwaI ATTTAAAT 1 cut(s) 1843
TaaI ACNGT 4 cut(s) 973, 1198, 1390, 1624
TaiI ACGT 2 cut(s) 502, 1330
TaqI TCGA 5 cut(s) 65, 102, 580, 2009, 2135
TaqII GACCGA 2 cut(s) 345, 533
TatI WGTACW 1 cut(s) 2049
TauI GCSGC 2 cut(s) 1224, 1452
Tru1I TTAA 6 cut(s) 180, 624, 744, 1275, 1842, 2061
Tru9I TTAA 6 cut(s) 180, 624, 744, 1275, 1842, 2061
TscAI CASTG 4 cut(s) 761, 978, 1287, 1309
TseFI GTSAC 4 cut(s) 514, 1258, 1367, 1579
TseI GCWGC 3 cut(s) 713, 1347, 1880
Tsp45I GTSAC 4 cut(s) 514, 1258, 1367, 1579
TspGWI ACGGA 2 cut(s) 528, 1512
TspMI CCCGGG 1 cut(s) 169
TspRI CASTG 4 cut(s) 761, 978, 1287, 1309
VpaK11BI GGWCC 2 cut(s) 184, 1851
XapI RAATTY 6 cut(s) 244, 442, 839, 1338, 1605, 1891
XceI RCATGY 4 cut(s) 432, 663, 1065, 1929
XmaI CCCGGG 1 cut(s) 169
XmnI GAANNNNTTC 2 cut(s) 1295, 1761
XspI CTAG 3 cut(s) 600, 1566, 1722
Zsp2I ATGCAT 1 cut(s) 2098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.