pycom02g02520

EIN3-binding F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002920
Physical Location & Seq
Reverse (-)
1563593 .. 1564636
1044 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g02520.1

Sequence Viewer

Length: 1044 bp
ATGAAGGTAAGGCTTCAGGCATTGAACATCACAGATTTCTCTCTTGCCGTGATTGGACATTATGGCAAGGCTGTTACCAATTTGTTTCTCAGTGGTCTCCAGAATGTGAGTGAGAAGGGCTTTTGGGTCATGGGTAATGCTCAAGCTCTGCAGGATCTGGCTTCATTAACAATCACATCTTGCCGGGGAACCACAGATGTCAGTCTCGAAGCCATTGGCAAGGGATGCACAAATTTGAAGCAGATGTGTCTTCGCAAGTGTTGCTTTGTATCCGACAACGGGCTGGCAGCTTTTGCCAAAGCTGCAGGATCACTTGAGAGCCTGCAACTGGAGGAGTGCAACAGAGTTACCCAATCAGGGATAATTTCTGCCCTCTCGAACTGCGGAGAAAAGCTGAGATCTCTTACCCTAGTGAAGTGCATGGGAATCAAGGATGCAGGTGCAGCAGTTCCTATCCCCTCTAGTTGCAGATCGCTTCGATCCTTGTCCATCCGAAACTGCCCTGGGTTCGGTAGTGCTAGCCTGGCCATGGTGGGAAGGTTGTGCCCTCAGCTCCATAATGTAGACATGAGTGGTCTATATGGAATGACAGATGCAGGGGTTCTGTCACTTCTTGGGAGCTTGGAGGAAGGGCTTGTCAAAGTGAATCTTAACGGATGCTTGAATTTGACAGATGAAGTTGTTATGGCATTGGTTAGGCTACATGGGGAAACTTTAGAAGTACTGAATCTTGATGGTTGCAGAAAGATTACTGATGCAAGCTTGGCGACAATCGCAAGCAACTGCTTGTTTCTTCGTGAGCTAGATGTGTCGAAGTGTGCGATTACAGATTCCGGCCTTGCTGCCCTTTCTTCCGCAGATCAGATCAACTTGCAAGTCCTTTCCATTTCTGGCTGTTCTGAAATCTCACACAAAAGCCTCCCTTCCCTGAAAAAATTAGGCCAGACCCTGGTGGGCTTGAATCTCCAACATTGTACTGCACTCAGCAACAGATCAATTGAGCTACTTGTGGAGAGCTTGTGGAGATGCGATATCTTGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

348

Amino Acids

36.71

Weight (kDa)

7.4

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 5 - 99 5.7e-08 Leucine Rich Repeat Domain of unknown function (DUF7885)
DUF7885 PF25372 90 - 180 1.8e-08 Leucine Rich Repeat Domain of unknown function (DUF7885)
DUF7885 PF25372 182 - 339 2.2e-12 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 428
Acc36I ACCTGC 1 cut(s) 428
AccB7I CCANNNNNTGG 1 cut(s) 949
AccI GTMKAC 1 cut(s) 564
AciI CCGC 2 cut(s) 384, 855
AclWI GGATC 3 cut(s) 162, 316, 474
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 2 cut(s) 232, 664
AfaI GTAC 2 cut(s) 723, 976
AfiI CCNNNNNNNGG 6 cut(s) 279, 328, 357, 509, 529, 949
AgsI TTSAA 4 cut(s) 25, 238, 664, 961
AjnI CCWGG 3 cut(s) 502, 522, 948
Alw26I GTCTC 2 cut(s) 101, 209
AlwI GGATC 3 cut(s) 162, 316, 474
AlwNI CAGNNNCTG 2 cut(s) 157, 949
AoxI GGCC 4 cut(s) 525, 835, 940, 1038
ApeKI GCWGC 4 cut(s) 287, 302, 443, 842
ApoI RAATTY 2 cut(s) 232, 664
AsuC2I CCSGG 1 cut(s) 185
AsuNHI GCTAGC 1 cut(s) 518
BaeGI GKGCMC 1 cut(s) 548
BalI TGGCCA 1 cut(s) 527
BbsI GAAGAC 1 cut(s) 242
BbvCI CCTCAGC 1 cut(s) 549
BbvI GCAGC 4 cut(s) 289, 299, 455, 829
BccI CCATC 2 cut(s) 497, 728
BceAI ACGGC 1 cut(s) 32
BciT130I CCWGG 3 cut(s) 504, 524, 950
BciVI GTATCC 1 cut(s) 280
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 2 cut(s) 101, 209
BfaI CTAG 4 cut(s) 410, 462, 519, 803
BfmI CTRYAG 2 cut(s) 149, 303
BfuAI ACCTGC 1 cut(s) 428
BfuI GTATCC 1 cut(s) 280
BglII AGATCT 1 cut(s) 398
BisI GCNGC 4 cut(s) 288, 303, 444, 843
BlsI GCNGC 4 cut(s) 289, 304, 445, 844
BmcAI AGTACT 1 cut(s) 723
Bme1390I CCNGG 4 cut(s) 185, 504, 524, 950
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 4 cut(s) 185, 504, 524, 950
BmsI GCATC 6 cut(s) 215, 424, 583, 647, 745, 1016
BmtI GCTAGC 1 cut(s) 522
BpiI GAAGAC 1 cut(s) 242
BpmI CTGGAG 2 cut(s) 83, 350
Bpu10I CCTNAGC 1 cut(s) 549
BpuEI CTTGAG 2 cut(s) 126, 335
BpuMI CCSGG 1 cut(s) 185
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 5 cut(s) 184, 502, 503, 528, 948
BsaXI ACNNNNNCTCC 2 cut(s) 1004, 1034
Bsc4I CCNNNNNNNGG 6 cut(s) 279, 328, 357, 509, 529, 949
Bse1I ACTGG 1 cut(s) 333
BseBI CCWGG 3 cut(s) 504, 524, 950
BseDI CCNNGG 5 cut(s) 184, 502, 503, 528, 948
BseGI GGATG 4 cut(s) 230, 439, 489, 662
BseLI CCNNNNNNNGG 6 cut(s) 279, 328, 357, 509, 529, 949
BseMII CTCAG 4 cut(s) 103, 386, 563, 997
BseNI ACTGG 1 cut(s) 333
BseRI GAGGAG 1 cut(s) 347
BseSI GKGCMC 1 cut(s) 548
BseXI GCAGC 4 cut(s) 289, 299, 455, 829
BsgI GTGCAG 2 cut(s) 462, 963
BshFI GGCC 4 cut(s) 527, 837, 942, 1040
BsiSI CCGG 2 cut(s) 184, 834
BslI CCNNNNNNNGG 6 cut(s) 279, 328, 357, 509, 529, 949
BsmAI GTCTC 2 cut(s) 101, 209
BsnI GGCC 4 cut(s) 527, 837, 942, 1040
Bso31I GGTCTC 1 cut(s) 101
Bsp1286I GDGCHC 1 cut(s) 548
Bsp143I GATC 8 cut(s) 154, 308, 398, 470, 479, 859, 864, 992
Bsp19I CCATGG 1 cut(s) 528
BspACI CCGC 2 cut(s) 384, 855
BspANI GGCC 4 cut(s) 527, 837, 942, 1040
BspCNI CTCAG 4 cut(s) 102, 387, 562, 996
BspLI GGNNCC 1 cut(s) 190
BspMAI CTGCAG 2 cut(s) 153, 307
BspMI ACCTGC 1 cut(s) 428
BspOI GCTAGC 1 cut(s) 522
BspPI GGATC 3 cut(s) 162, 316, 474
BspTNI GGTCTC 1 cut(s) 101
BsrI ACTGG 1 cut(s) 333
BssECI CCNNGG 5 cut(s) 184, 502, 503, 528, 948
BssMI GATC 8 cut(s) 154, 308, 398, 470, 479, 859, 864, 992
BssT1I CCWWGG 1 cut(s) 528
Bst2UI CCWGG 3 cut(s) 504, 524, 950
BstAPI GCANNNNNTGC 3 cut(s) 225, 261, 293
BstC8I GCNNGC 5 cut(s) 285, 323, 520, 760, 778
BstDEI CTNAG 4 cut(s) 89, 395, 549, 983
BstDSI CCRYGG 1 cut(s) 528
BstF5I GGATG 4 cut(s) 230, 439, 489, 662
BstKTI GATC 8 cut(s) 157, 311, 401, 473, 482, 862, 867, 995
BstMAI GTCTC 2 cut(s) 101, 209
BstMBI GATC 8 cut(s) 154, 308, 398, 470, 479, 859, 864, 992
BstMWI GCNNNNNNNGC 8 cut(s) 225, 261, 293, 302, 443, 524, 764, 773
BstNI CCWGG 3 cut(s) 504, 524, 950
BstSCI CCNGG 4 cut(s) 183, 502, 522, 948
BstSFI CTRYAG 2 cut(s) 149, 303
BstSLI GKGCMC 1 cut(s) 548
BstV1I GCAGC 4 cut(s) 289, 299, 455, 829
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 2 cut(s) 154, 398
BstYI RGATCY 2 cut(s) 154, 398
BsuI GTATCC 1 cut(s) 280
BsuRI GGCC 4 cut(s) 527, 837, 942, 1040
BtgI CCRYGG 1 cut(s) 528
BtsCI GGATG 4 cut(s) 230, 439, 489, 662
BtsIMutI CAGTG 1 cut(s) 97
BveI ACCTGC 1 cut(s) 428
Cac8I GCNNGC 5 cut(s) 285, 323, 520, 760, 778
CaiI CAGNNNCTG 2 cut(s) 157, 949
Csp6I GTAC 2 cut(s) 722, 975
CviAII CATG 5 cut(s) 130, 421, 529, 568, 704
CviQI GTAC 2 cut(s) 722, 975
DdeI CTNAG 4 cut(s) 89, 395, 549, 983
DpnI GATC 8 cut(s) 156, 310, 400, 472, 481, 861, 866, 994
DpnII GATC 8 cut(s) 154, 308, 398, 470, 479, 859, 864, 992
EaeI YGGCCR 1 cut(s) 525
Eco130I CCWWGG 1 cut(s) 528
Eco31I GGTCTC 1 cut(s) 101
Eco32I GATATC 1 cut(s) 1033
EcoRII CCWGG 3 cut(s) 502, 522, 948
EcoRV GATATC 1 cut(s) 1033
EcoT14I CCWWGG 1 cut(s) 528
ErhI CCWWGG 1 cut(s) 528
FaeI CATG 5 cut(s) 133, 424, 532, 571, 707
FalI AAGNNNNNCTT 6 cut(s) 248, 280, 633, 665, 907, 939
FatI CATG 5 cut(s) 129, 420, 528, 567, 703
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 4 cut(s) 288, 303, 444, 843
FokI GGATG 4 cut(s) 237, 446, 476, 669
Fsp4HI GCNGC 4 cut(s) 288, 303, 444, 843
FspBI CTAG 4 cut(s) 410, 462, 519, 803
GluI GCNGC 4 cut(s) 288, 303, 444, 843
GsuI CTGGAG 2 cut(s) 83, 350
HaeIII GGCC 4 cut(s) 527, 837, 942, 1040
HapII CCGG 2 cut(s) 184, 834
Hin1II CATG 5 cut(s) 133, 424, 532, 571, 707
HindIII AAGCTT 1 cut(s) 760
HinfI GANTC 5 cut(s) 426, 646, 727, 830, 961
HpaII CCGG 2 cut(s) 184, 834
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 4 cut(s) 274, 494, 864, 901
Hpy188III TCNNGA 5 cut(s) 100, 206, 376, 731, 797
Hpy8I GTNNAC 1 cut(s) 565
HpyAV CCTTC 4 cut(s) 109, 531, 623, 933
HpyF10VI GCNNNNNNNGC 8 cut(s) 225, 261, 293, 302, 443, 524, 764, 773
HpyF3I CTNAG 4 cut(s) 89, 395, 549, 983
Hsp92II CATG 5 cut(s) 133, 424, 532, 571, 707
Kzo9I GATC 8 cut(s) 154, 308, 398, 470, 479, 859, 864, 992
LmnI GCTCC 2 cut(s) 558, 618
Lsp1109I GCAGC 4 cut(s) 289, 299, 455, 829
LweI GCATC 6 cut(s) 215, 424, 583, 647, 745, 1016
MaeI CTAG 4 cut(s) 410, 462, 519, 803
MaeIII GTNAC 3 cut(s) 73, 346, 606
MalI GATC 8 cut(s) 156, 310, 400, 472, 481, 861, 866, 994
MboI GATC 8 cut(s) 154, 308, 398, 470, 479, 859, 864, 992
MboII GAAGA 3 cut(s) 242, 785, 843
MfeI CAATTG 1 cut(s) 996
MflI RGATCY 2 cut(s) 154, 398
MhlI GDGCHC 1 cut(s) 548
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 6 cut(s) 79, 232, 363, 664, 935, 996
MluNI TGGCCA 1 cut(s) 527
MmeI TCCRAC 2 cut(s) 297, 991
MnlI CCTC 6 cut(s) 325, 383, 469, 558, 619, 929
Mox20I TGGCCA 1 cut(s) 527
MscI TGGCCA 1 cut(s) 527
MseI TTAA 2 cut(s) 167, 651
Msp20I TGGCCA 1 cut(s) 527
MspI CCGG 2 cut(s) 184, 834
MspR9I CCNGG 4 cut(s) 185, 504, 524, 950
MunI CAATTG 1 cut(s) 996
MvaI CCWGG 3 cut(s) 504, 524, 950
MwoI GCNNNNNNNGC 8 cut(s) 225, 261, 293, 302, 443, 524, 764, 773
NciI CCSGG 1 cut(s) 185
NcoI CCATGG 1 cut(s) 528
NdeII GATC 8 cut(s) 154, 308, 398, 470, 479, 859, 864, 992
NheI GCTAGC 1 cut(s) 518
NlaIII CATG 5 cut(s) 133, 424, 532, 571, 707
NlaIV GGNNCC 1 cut(s) 190
NmuCI GTSAC 1 cut(s) 606
PaqCI CACCTGC 1 cut(s) 428
PasI CCCWGGG 1 cut(s) 503
PcsI WCGNNNNNNNCGW 1 cut(s) 818
PfeI GAWTC 5 cut(s) 426, 646, 727, 830, 961
PflMI CCANNNNNTGG 1 cut(s) 949
PkrI GCNGC 4 cut(s) 289, 304, 445, 844
Psp6I CCWGG 3 cut(s) 502, 522, 948
PspGI CCWGG 3 cut(s) 502, 522, 948
PspN4I GGNNCC 1 cut(s) 190
PstI CTGCAG 2 cut(s) 153, 307
PstNI CAGNNNCTG 2 cut(s) 157, 949
PsuI RGATCY 2 cut(s) 154, 398
RsaI GTAC 2 cut(s) 723, 976
RsaNI GTAC 2 cut(s) 722, 975
SaqAI TTAA 2 cut(s) 167, 651
SatI GCNGC 4 cut(s) 288, 303, 444, 843
Sau3AI GATC 8 cut(s) 154, 308, 398, 470, 479, 859, 864, 992
ScaI AGTACT 1 cut(s) 723
ScrFI CCNGG 4 cut(s) 185, 504, 524, 950
SduI GDGCHC 1 cut(s) 548
SfaNI GCATC 6 cut(s) 215, 424, 583, 647, 745, 1016
SfcI CTRYAG 2 cut(s) 149, 303
SmlI CTYRAG 2 cut(s) 141, 314
SmoI CTYRAG 2 cut(s) 141, 314
Sse9I AATT 6 cut(s) 79, 232, 363, 664, 935, 996
SsiI CCGC 2 cut(s) 384, 855
SspMI CTAG 4 cut(s) 410, 462, 519, 803
StyD4I CCNGG 4 cut(s) 183, 502, 522, 948
StyI CCWWGG 1 cut(s) 528
TaqI TCGA 4 cut(s) 207, 377, 478, 812
TasI AATT 6 cut(s) 79, 232, 363, 664, 935, 996
TatI WGTACW 2 cut(s) 721, 974
TfiI GAWTC 5 cut(s) 426, 646, 727, 830, 961
Tru1I TTAA 2 cut(s) 167, 651
Tru9I TTAA 2 cut(s) 167, 651
TscAI CASTG 1 cut(s) 97
TseFI GTSAC 1 cut(s) 606
TseI GCWGC 4 cut(s) 287, 302, 443, 842
Tsp45I GTSAC 1 cut(s) 606
TspDTI ATGAA 3 cut(s) 17, 153, 690
TspGWI ACGGA 1 cut(s) 669
TspRI CASTG 1 cut(s) 97
Van91I CCANNNNNTGG 1 cut(s) 949
XapI RAATTY 2 cut(s) 232, 664
XmiI GTMKAC 1 cut(s) 564
XspI CTAG 4 cut(s) 410, 462, 519, 803
ZrmI AGTACT 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.