Rorug06G0277500

Protein of unknown function (DUF4050)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002920
Physical Location & Seq
Forward (+)
42518033 .. 42531057
13025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0277500.1

Sequence Viewer

Length: 6042 bp
ATGCATGGATGTGGCTCAGGATCTGCTCTCCTAGTAAATGCAGAGGTGGATTCCATGGGTGGAGTGGTTGACGGCGGAGTTGGTGTTGGTTTGAAAACATCTCCGCGCCGAGCAGCAATTGAGAAGGCTCAAGCAGAACTTAGACAGGAATATGATGTGCGGGAGGAAAGGAGAAGGGAGCTAGAATTTCTTGAGAAAGGTGGCAATCCTTTGGACTTCAAATTTGGGAATGCAGCTTCTGTTAGTGTCCAGTCTACTTCCCTCACTGATCAGCATCCAGAACAGTTTGTGACCAGTGAAGCAAAAGGTAGTTTTGCATTGACTGCATCACCTCGTGGGGACTCTGTCGAAAGTAGTGGTCGACCAGAGGTTCCTACACTTTGTGAACCCAACAGTGCTGATAATCTCTTACTTTTTGATGGGGACAATGATACACCGGAAGGGGAAAGGAAATCTATGCATATTAGTAGAAGAAATAAAATTGCTCCATCAGAACAGTCTTCCCAGATGGATGGGACTCAAAATGCCAAGGAATCAGAAGACTCAGCGATTTTCCGTCCTTATGCTCGAAGGAACAGGTCCAGACCAAATCGTGATGGTACTCGTTCAAGTTCAACTGATGTACAGGGTCGTGGTGGTCAAGGGTCTTCATTACCTTCTCGCGGGACATTAAAGAATCCCAAGGGACCGGTATCTGAAACAAACAACCAAAAGGACCAGAATATACCTCCGGTCACTAATCTAAAGTCTGTGAAGTCTAATGGTGATTTTGCTCCTAGGGTTGCAACTTCTGATAATCAGTTGGACATGGACTTTGATGGGGTGCAGGCTCCTGAAATATTTACTGGTCCAGCAAAAGATAGTCCTGAAAGAAAATTGGATGTTACAGCTACTGAAAGTTTGAAAGAGAGCCAACATAGTCAACCATCTCAGACTGATACTCAGGAAATTCCAATTGCTGCGGTTTCGGGGAGATCTGATGAGAGGGAGCCGTTAGCTTCGTCTGTTCTTGAATGTCTTCGTTGTGAAGCTACTACAAAGACCGAAATTGATATTAGTTCTGTCCAGGTGAATGGATTTAGTAATTTAAATAGAGAGAGTAAAAGTGTACCAAATGAAGGTCAAATTAGCAGTGCAGCGGGCACAAAGGGGTTAGATTCAGAATCTTCTTGTACCCAAACTAGTGTAGGATTAGATGTAAATAATGATACTGACGTATGTACCACAAGGAATGCTGATAATGCAAATATCATGGAAACATCAGATGTTGATGGGGCACAAAATCCAGCTGGTGATGAAATGTTACTAGAAAAGAATGAAAGCAGAGCTGTTGACAGTAGTCCCATGATTAATGATCCTCATGCTTCTGATTTTCAGAACAATCGCTCAGGCAATACTGAAGTGAAAGTTGAGGAAGATATGAACGAAAGTAGATCTGAAGTGCGTAATGAAGTAAAGCTTCATCCAAACACTGAGGGAGACCAACAAAGTGGTTGTACTGTATCAGAAGCTGAAAAGAAACTGGATGATGTGGTGGATAATGGTTCCAACATCAAGAAAGAGAACTCTTCTGGCAGACCCCAGAATCCTCAGGATTTATCTATGTGTGAGCTTCCTGAAACTATTTTGTCAGGGATAGACTCTGCCAAGGGTTCAGACTGTCAGACTTCTAGTAGTCACTTTAAAGTGGTAGACAAGGCACATGAAGATTCTATTTTGGAAGAGGCTCGGATGATAGAGGCTAAGCGTAAGAGGATTGCAGAATTATCCGTTCGTTCTTTACCCTCGGAGAATCACCGAAAGTCTCAGTGGGATTTTGTCCTTGAGGAAATGTCGTGGTTGGCCAATGATTTTGCTCAGGAGCGCCTTTGGAAGCTAACTGCTGCTGCTCAAATATGCCATCGTGTTGCTTTTACATCACGGTTGAGAATTGAAGAGCAACAACAACAATGGGGGATTAAAAAAGTGGCTCGTACCCTGGCAAATGCTGTCAACCAATTTTGGCATTCAGCTGGGACCCTTTTGTATGCTGATGATTCAAGTGATTGTCACAACATCACCAAGAACAATTTAAATTCGAGCAAAGAGCCTTGCAAAGAGTTGGAGCTACAATGGAGCAACAACTTTTCACTTTCTATGCAGCGGTATGCAGTGAGATTTTTGAAGTATAACAACTCTCTTGGTCCTCAACTTCAAGCACAAGCTCCTGCTACTCCTGAGAGATTGTCTGATTTAGGCATTACAGAAATGTCATGGGAGGACCATCTTACAGAAGAAAACCTCTTTTATGCAGTTCCATCTGGGGCACTGGAAACCTACAGAAGATCAATTGAATCTCATTTGGTTCAGTGTGAGAGAACTAGCAGTAGCATGCAAGAAGAGGTTGATACATCCACGTATGAGGCTGGAGCTGAGTTTGATAGTCGAGAGACTTCTGCATACGATGAGGATGAAGGAGAAACAACATGCACCTATTATTTGCCTGGGGCCTTCGAAGGTAGCAAGTCATCAACATTTAATCAGAAGAAGCGGAAGTATTATAAGTCATATAATCCTAGGACACATGAAGCAGGAGCTGATTTTCCCTATGGACAAACAGCAAATCAACAGCCCATGTTGATGGGGAAAAGGCCTGCTAGTCTTAATGTTGGTTCAATCCCAACAAAACGCGTGCGCACTGCTTCCAGGCAGAGGGTTGTTAGTCCATTTGGTGCTGGAGCTACTGGGAATGCTCAGGCTCAAATTAAGACAGATGCTTCTAGTGGAGATACTAATTCTTTTCAGGATGATCAGAGTACCTTACCTGGCGGATCCCAGTTCCCTAGAAATATGGAGGTGGAGTCTGTTCGTGATTTTGAAAAGCATCCACAATGTGACTATCCAGAAACATCAAAGCATAAAAAGAAGAAGAAGGCAAAACATCTGGGTTCCGCTTATGACCAAGTATGGCAGGTGGATTCACCTACTCATAATGAGCAGAGGGATCATTCAAAAAAGAGATTAGAAAGTCATCATTTTGAGTCCAATGGAACAATTGGTTTATATGGGCAACATAATGCAAAGAGGCTGAAGATATCAAAACAGTCTCTAGACAATACTTATGACGGTATAACTCCAATGACTGGGTCCATTCCTTCTCCAGTGGCGTCCCAAATGAGTAATATGACCAATCCGAGTAAATTGATGAAGTTGATCGGTGGTCGGGACAAGGGACGGAAAGCTAAATCAATGAAGATGCCTGTTGGGCACCCAGGTTCTGGAAGTCCATGGTCACTATTTGAAGATCAGGCACTTGTTGTTCTTGTACACGATATGGGTCCAAATTGGGAACTCATAAGTGATGCTATCAACAGTACTCTGCACCTAAAGTGTATCTTCCGCAAGCCTAAGGAATGCAAGGAGCGTCACAAAATTTTAATGGATTTGAATACTGGTGATGGGGCTGACAGTGCTGAAGATTCAGGGTCATCACAGCCTTATCCATCTACAATCCCTGGCATTCCAAAGGGAAGTGCCAGACAGTTATTTCAACGCTTGCAAGAGCCAATGGAAGAGGACACTCTCAAGTCTCATTTTGAAAGAATAATTAAGATTGGGCAGAAGCATCATTATAGGAGGAGTCAGAGTGATAACCAGGATCCAAAACAAGTAACTACAGTCCACAATTCTCATGTTATCGCTCTTTCCCAAGTATGTCCGAATAACCTGAATGGAGTTATTCTAACGCCTCTTGATCTCTGTGATGCCACATCATCAAGCCCAGATGTTCTTCCCACTGCATATCAAGGTTCTCACACTAGTGGCTTACCAATGGCAAATCAGGGCGCTATGGCGTCATTACTTCCCTCTGGGCCAAATGCCTCATTACAGGGAACTTCTGGTATGGTTCTTGGTAGCAACTTGTCATCACCATCTGGCCCGCTTAGTGCGACAGTCAGGGATGGTAGGTACGGTGGTCCAAGGGCATCTACTTTACCAGTTGAAGAGCAGCAAAGAATGCAACACTACAATCAAATGTTATCTGGTAGAAACATTCAGCAGCCCAGCTTGTCTGTACCTGGGGCTCTTCCAGGAGGTACTGATCGTGGTGTTCGAATGGTACCTGGTGCAAATGGTATGGGCATGATGTGTGGGATGAATAGAAGCACAATGTCAAGGCCAGGGTTTCAGGGAATCGCTTCTTCCTCGATGCTGAATACTGGGAGTATGCTTTCATCCAGTATGGTTGGAATACCAAGTCCTGTAAATATGCACTCTGGAGCTGGTTCTGGTCCAGGAAATTTGATGTTAAGACCTCGCGAGGGTCACATGATGCGGCCTGCCCATAATCCGGATCACCAAAGGCAACTTATGGCTCCAGAGCTTCAGATGCAGGTCACGCAAGGGAATGGCCAAGGTATTGCTCCATTCAATGGGTTGAGTTCTGGCTTTCCTAGTCAGACAACTCCATCAGGTGCACAAATGTATCCAGGCCATCCCCAGCAGCAACATCAGTTATCCCCACAACAATCTCATGCAGTCGGCAGCCCTCATCACCCTCATCTTCAAGGCCCCAATCATGTGACAGGGTCGCAGCAGCAAGCCTATGCCATGCGCATGGCTAAAGAAAGGCACCTGCATCAACAGCGGTTTTTGCAGCAGCAGCAGCAGCAACAGTTTGCATCATCCAATTCTTTGGTGCCGCATGTCCAACCACAGGCCCAACTCCCCATTTCTTCAACTTTGCAAAATAGTTCCCAGATTCAGTCACAAAGTTCACCTCACCCAGCATCTATGTCCCCTAGTACACCTTCTTCCCCAATGACTCCGGTATCATCCCAACACCAGCAGAAACATCACCTGCCACCTCATGGGATGAGTCGGAATCCTGGTGCAAGCGGGTTGACTAATCAAATAGGGAAGCAACGACAAAGGCCACAACAACACCATCTTCAGCAATCTGGGAGGCACCACCCTCAGCAGCGGCCATTTGGGCAATCTCAGCAGCAGGCTAAACTTTCGAAGGGAATGGGAAGAGGGAATCCAATGATGCATCAAAACCTTGCCATCGATCCTATAAACATCTCCATTGATCCATCTCATCTGAATGGGCTTTCGATGCCTCCGGGAAGTCAAGCTCTGGAGAAAGGAGAAAAGATCATGCAGTTAATGCAAGGTCAAGCTGCATATTCGGGGTCTGGCGTCAACCCAGTAACATCAAAACCATTGGTTCCTCAATCATCAAACAACTCTCCAATGCAGCAAAAGTTGCATTCTAGCTCAGCTACCTCTTCGACGAAGCAACTCCAGCAAAAGCCATCTCATTCTGATAACAGCACTCAAGGCCAGGCACCAGCTGTTCCTTCTGGTCATGCAATATCAGCTTCACATCAATCTGTGTCTCCAGCAATTGTGTCTTCCAATCACCAGCAGGTGCAGCCACAGCAACAGCAACAGAAGCAAGCTAATCAGACTCAGCCATATGTTCAGAGAGTCCAGCAGAATCGTCAAGTGAATTCGGAGCTTCCAAGCAAGCCTCCAAATGATTTAGCTTCAGCCGAAGAGCAGCCTGTGAATAGTACTTCTCTGATCGGTTCGAGCATGGCAATTCCTCAGTCCTCTATTGATTCGTCTAATGTAGTACCGGTTTCTTCTGGCATTACTCAATGGAAATCATCAGAAGCAGTATATGATTCTAATCTGCCAAATTCAACAGCTCAAGAGGGTTCACTGGGGAGCCCATCACCAACAAATTCATCTGGGAATGAGCCAGTGCCCCCATTTAGTCAAGGTTTAGGCCCAAGGCAGTTATCTGGTAACTTTGCCTCTCATGGACATATTGCTGGTGCGCAATGGCAGCAGAAGCAGCTACTGCAAAAGTCTCCTTCGCTACCACCCCCGTCCCAACAACTCTACGAGCAACAAGAGCAACAGCAGCAGCAGGAACAAGAATCACCCCAACATCAACTGCCTTTGCCACAGCAGTCTCAGCAACAAATGCAGCATCTGCCAGCAGGGCAAGGTGGTTTGTATATGATGCCTAGTAATTCAAAATCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

2013

Amino Acids

219.16

Weight (kDa)

6.46

Isoelectric Point (pI)

62.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_6 PF13921 1090 - 1138 3.3e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2541
AarI CACCTGC 4 cut(s) 2941, 4623, 4848, 5401
Acc16I TGCGCA 3 cut(s) 2675, 4595, 5829
Acc36I ACCTGC 5 cut(s) 2941, 4363, 4623, 4848, 5401
Acc65I GGTACC 1 cut(s) 4099
AccB1I GGYRCC 6 cut(s) 3246, 4099, 4611, 4678, 4947, 5329
AccB7I CCANNNNNTGG 4 cut(s) 3122, 3257, 4412, 4850
AccI GTMKAC 3 cut(s) 254, 361, 1692
AccII CGCG 4 cut(s) 106, 663, 2670, 4299
AccIII TCCGGA 1 cut(s) 4330
AcoI YGGCCR 3 cut(s) 1842, 4390, 4964
AcsI RAATTY 9 cut(s) 185, 221, 948, 2074, 3411, 4279, 5494, 5686, 5731
AcuI CTGAAG 7 cut(s) 1419, 1458, 3089, 3474, 4349, 4916, 5517
AcyI GRCGYC 3 cut(s) 3146, 3833, 5181
AdeI CACNNNGTG 3 cut(s) 335, 383, 2873
AflIII ACRYGT 1 cut(s) 2668
AgeI ACCGGT 2 cut(s) 688, 5623
AhlI ACTAGT 2 cut(s) 1181, 3797
AleI CACNNNNGTG 1 cut(s) 3798
Alw21I GWGCWC 1 cut(s) 4459
Alw26I GTCTC 8 cut(s) 1473, 1809, 2423, 3090, 3573, 5385, 5865, 5970
Alw44I GTGCAC 1 cut(s) 4455
AlwNI CAGNNNCTG 8 cut(s) 23, 239, 893, 1511, 2576, 3257, 5851, 5986
Aor13HI TCCGGA 1 cut(s) 4330
ApaLI GTGCAC 1 cut(s) 4455
ApoI RAATTY 9 cut(s) 185, 221, 948, 2074, 3411, 4279, 5494, 5686, 5731
ArsI GACNNNNNNTTYG 4 cut(s) 206, 238, 797, 829
AseI ATTAAT 1 cut(s) 1350
AsiGI ACCGGT 2 cut(s) 688, 5623
Asp700I GAANNNNTTC 2 cut(s) 1017, 4177
Asp718I GGTACC 1 cut(s) 4099
AspA2I CCTAGG 2 cut(s) 776, 2555
AspLEI GCGC 6 cut(s) 108, 1866, 2676, 3827, 4596, 5830
AsuC2I CCSGG 1 cut(s) 5106
AsuII TTCGAA 3 cut(s) 2493, 4093, 5000
AvrII CCTAGG 2 cut(s) 776, 2555
AxyI CCTNAGG 2 cut(s) 1590, 3387
BaeGI GKGCMC 6 cut(s) 1145, 1279, 2308, 3249, 4459, 5757
BalI TGGCCA 2 cut(s) 1844, 4392
BamHI GGATCC 2 cut(s) 2810, 3637
BanI GGYRCC 6 cut(s) 3246, 4099, 4611, 4678, 4947, 5329
BanII GRGCYC 2 cut(s) 4066, 5720
BarI GAAGNNNNNNTAC 2 cut(s) 4777, 4809
BauI CACGAG 1 cut(s) 333
BbsI GAAGAC 5 cut(s) 492, 546, 639, 1010, 5388
Bbv12I GWGCWC 1 cut(s) 4459
BbvCI CCTCAGC 1 cut(s) 4956
BceAI ACGGC 2 cut(s) 88, 976
BciVI GTATCC 1 cut(s) 4476
BclI TGATCA 2 cut(s) 268, 2788
BcnI CCSGG 1 cut(s) 5106
BcoDI GTCTC 8 cut(s) 1473, 1809, 2423, 3090, 3573, 5385, 5865, 5970
BcuI ACTAGT 2 cut(s) 1181, 3797
BfmI CTRYAG 2 cut(s) 2317, 3654
BfoI RGCGCY 2 cut(s) 1867, 3828
BfuAI ACCTGC 5 cut(s) 2941, 4363, 4623, 4848, 5401
BfuI GTATCC 1 cut(s) 4476
BglI GCCNNNNNGGC 3 cut(s) 3244, 4972, 5995
BglII AGATCT 2 cut(s) 974, 1433
BlnI CCTAGG 2 cut(s) 776, 2555
BlpI GCTNAGC 2 cut(s) 1743, 5260
BmcAI AGTACT 2 cut(s) 3355, 5560
BmrI ACTGGG 6 cut(s) 2733, 2809, 3132, 4209, 5183, 5720
BmuI ACTGGG 6 cut(s) 2733, 2809, 3132, 4209, 5183, 5720
BoxI GACNNNNGTC 1 cut(s) 1338
BpiI GAAGAC 5 cut(s) 492, 546, 639, 1010, 5388
BpmI CTGGAG 8 cut(s) 2427, 2736, 3123, 4278, 4341, 5141, 5270, 5367
Bpu10I CCTNAGC 5 cut(s) 16, 1387, 1857, 2733, 4956
Bpu1102I GCTNAGC 2 cut(s) 1743, 5260
Bpu14I TTCGAA 3 cut(s) 2493, 4093, 5000
BpuEI CTTGAG 6 cut(s) 114, 212, 1844, 3548, 5304, 5682
BpuMI CCSGG 1 cut(s) 5106
Bsa29I ATCGAT 1 cut(s) 5049
BsaAI YACGTR 1 cut(s) 2397
BsaBI GATNNNNATC 2 cut(s) 273, 5676
BsaHI GRCGYC 3 cut(s) 3146, 3833, 5181
BsaI GGTCTC 1 cut(s) 1473
BsaWI WCCGGW 6 cut(s) 436, 688, 730, 4330, 4807, 5623
BsaXI ACNNNNNCTCC 6 cut(s) 69, 99, 2825, 2855, 3610, 3640
Bse118I RCCGGY 2 cut(s) 688, 5623
Bse21I CCTNAGG 2 cut(s) 1590, 3387
Bse3DI GCAATG 1 cut(s) 5837
Bse8I GATNNNNATC 2 cut(s) 273, 5676
BseAI TCCGGA 1 cut(s) 4330
BseCI ATCGAT 1 cut(s) 5049
BseJI GATNNNNATC 2 cut(s) 273, 5676
BseMI GCAATG 1 cut(s) 5837
BseRI GAGGAG 1 cut(s) 3631
BseSI GKGCMC 6 cut(s) 1145, 1279, 2308, 3249, 4459, 5757
BseYI CCCAGC 4 cut(s) 2012, 4043, 4479, 4765
BsgI GTGCAG 4 cut(s) 845, 1155, 3344, 5435
Bsh1236I CGCG 4 cut(s) 106, 663, 2670, 4299
BshNI GGYRCC 6 cut(s) 3246, 4099, 4611, 4678, 4947, 5329
BshTI ACCGGT 2 cut(s) 688, 5623
BshVI ATCGAT 1 cut(s) 5049
BsiHKAI GWGCWC 1 cut(s) 4459
BsiSI CCGG 7 cut(s) 437, 689, 731, 4331, 4808, 5105, 5624
BsmAI GTCTC 8 cut(s) 1473, 1809, 2423, 3090, 3573, 5385, 5865, 5970
BsmI GAATGC 8 cut(s) 235, 1237, 2005, 2734, 3398, 3498, 4002, 5251
Bso31I GGTCTC 1 cut(s) 1473
Bsp119I TTCGAA 3 cut(s) 2493, 4093, 5000
Bsp1286I GDGCHC 8 cut(s) 1145, 1279, 2308, 3249, 4066, 4459, 5720, 5757
Bsp13I TCCGGA 1 cut(s) 4330
Bsp1407I TGTACA 2 cut(s) 622, 3304
Bsp1720I GCTNAGC 2 cut(s) 1743, 5260
Bsp19I CCATGG 2 cut(s) 54, 3266
Bsp68I TCGCGA 1 cut(s) 4299
BspDI ATCGAT 1 cut(s) 5049
BspEI TCCGGA 1 cut(s) 4330
BspFNI CGCG 4 cut(s) 106, 663, 2670, 4299
BspMI ACCTGC 5 cut(s) 2941, 4363, 4623, 4848, 5401
BspQI GCTCTTC 4 cut(s) 1929, 3978, 4071, 5535
BspT104I TTCGAA 3 cut(s) 2493, 4093, 5000
BspT107I GGYRCC 6 cut(s) 3246, 4099, 4611, 4678, 4947, 5329
BspTNI GGTCTC 1 cut(s) 1473
BsrDI GCAATG 1 cut(s) 5837
BsrFI RCCGGY 2 cut(s) 688, 5623
BsrGI TGTACA 2 cut(s) 622, 3304
BssAI RCCGGY 2 cut(s) 688, 5623
BssNI GRCGYC 3 cut(s) 3146, 3833, 5181
BssSI CACGAG 1 cut(s) 333
Bst2BI CACGAG 1 cut(s) 333
BstACI GRCGYC 3 cut(s) 3146, 3833, 5181
BstAPI GCANNNNNTGC 7 cut(s) 323, 3997, 5149, 5248, 5851, 5977, 5986
BstAUI TGTACA 2 cut(s) 622, 3304
BstBAI YACGTR 1 cut(s) 2397
BstBI TTCGAA 3 cut(s) 2493, 4093, 5000
BstDSI CCRYGG 2 cut(s) 54, 3266
BstFNI CGCG 4 cut(s) 106, 663, 2670, 4299
BstH2I RGCGCY 2 cut(s) 1867, 3828
BstHHI GCGC 6 cut(s) 108, 1866, 2676, 3827, 4596, 5830
BstMAI GTCTC 8 cut(s) 1473, 1809, 2423, 3090, 3573, 5385, 5865, 5970
BstNSI RCATGY 3 cut(s) 2374, 2469, 4688
BstPAI GACNNNNGTC 1 cut(s) 1338
BstSFI CTRYAG 2 cut(s) 2317, 3654
BstSLI GKGCMC 6 cut(s) 1145, 1279, 2308, 3249, 4459, 5757
BstUI CGCG 4 cut(s) 106, 663, 2670, 4299
BstV2I GAAGAC 5 cut(s) 492, 546, 639, 1010, 5388
BstX2I RGATCY 5 cut(s) 20, 974, 1433, 2810, 3637
BstXI CCANNNNNNTGG 6 cut(s) 512, 1073, 1490, 2620, 4597, 4675
BstYI RGATCY 5 cut(s) 20, 974, 1433, 2810, 3637
Bsu15I ATCGAT 1 cut(s) 5049
Bsu36I CCTNAGG 2 cut(s) 1590, 3387
BsuI GTATCC 1 cut(s) 4476
BsuTUI ATCGAT 1 cut(s) 5049
BtgI CCRYGG 2 cut(s) 54, 3266
BtsI GCAGTG 4 cut(s) 1138, 2157, 2676, 3774
BtuMI TCGCGA 1 cut(s) 4299
BveI ACCTGC 5 cut(s) 2941, 4363, 4623, 4848, 5401
CaiI CAGNNNCTG 8 cut(s) 23, 239, 893, 1511, 2576, 3257, 5851, 5986
CfoI GCGC 6 cut(s) 108, 1866, 2676, 3827, 4596, 5830
Cfr10I RCCGGY 2 cut(s) 688, 5623
ClaI ATCGAT 1 cut(s) 5049
CseI GACGC 4 cut(s) 3135, 3392, 3822, 5170
CsiI ACCWGGT 1 cut(s) 4102
CspAI ACCGGT 2 cut(s) 688, 5623
DraI TTTAAA 3 cut(s) 1089, 1684, 2073
DraIII CACNNNGTG 3 cut(s) 335, 383, 2873
EaeI YGGCCR 3 cut(s) 1842, 4390, 4964
EciI GGCGGA 2 cut(s) 90, 2823
Eco147I AGGCCT 1 cut(s) 2632
Eco24I GRGCYC 2 cut(s) 4066, 5720
Eco31I GGTCTC 1 cut(s) 1473
Eco32I GATATC 1 cut(s) 3075
Eco57I CTGAAG 7 cut(s) 1419, 1458, 3089, 3474, 4349, 4916, 5517
Eco81I CCTNAGG 2 cut(s) 1590, 3387
EcoO109I RGGNCCY 3 cut(s) 2016, 2487, 4550
EcoRI GAATTC 1 cut(s) 5494
EcoRV GATATC 1 cut(s) 3075
EcoT22I ATGCAT 3 cut(s) 6, 462, 5034
EcoT38I GRGCYC 2 cut(s) 4066, 5720
FalI AAGNNNNNCTT 6 cut(s) 123, 155, 1443, 1475, 3359, 3391
FauI CCCGC 5 cut(s) 153, 656, 1132, 3927, 4871
FauNDI CATATG 1 cut(s) 5461
FbaI TGATCA 2 cut(s) 268, 2788
FblI GTMKAC 3 cut(s) 254, 361, 1692
FriOI GRGCYC 2 cut(s) 4066, 5720
FspAI RTGCGCAY 2 cut(s) 2675, 4595
FspI TGCGCA 3 cut(s) 2675, 4595, 5829
GlaI GCGC 6 cut(s) 107, 1865, 2675, 3826, 4595, 5829
GsaI CCCAGC 4 cut(s) 2016, 4047, 4483, 4769
GsuI CTGGAG 8 cut(s) 2427, 2736, 3123, 4278, 4341, 5141, 5270, 5367
HaeII RGCGCY 2 cut(s) 1867, 3828
HapII CCGG 7 cut(s) 437, 689, 731, 4331, 4808, 5105, 5624
HgaI GACGC 4 cut(s) 3135, 3392, 3822, 5170
HhaI GCGC 6 cut(s) 108, 1866, 2676, 3827, 4596, 5830
Hin1I GRCGYC 3 cut(s) 3146, 3833, 5181
Hin6I GCGC 6 cut(s) 106, 1864, 2674, 3825, 4594, 5828
HinP1I GCGC 6 cut(s) 106, 1864, 2674, 3825, 4594, 5828
HincII GTYRAC 7 cut(s) 70, 362, 923, 1333, 1993, 4884, 5185
HindII GTYRAC 7 cut(s) 70, 362, 923, 1333, 1993, 4884, 5185
HindIII AAGCTT 1 cut(s) 1457
HpaII CCGG 7 cut(s) 437, 689, 731, 4331, 4808, 5105, 5624
Hpy99I CGWCG 1 cut(s) 5278
HpyCH4IV ACGT 2 cut(s) 1215, 2396
HpySE526I ACGT 2 cut(s) 1215, 2396
Hsp92I GRCGYC 3 cut(s) 3146, 3833, 5181
HspAI GCGC 6 cut(s) 106, 1864, 2674, 3825, 4594, 5828
KflI GGGWCCC 1 cut(s) 2016
Kpn2I TCCGGA 1 cut(s) 4330
KpnI GGTACC 1 cut(s) 4103
Ksp22I TGATCA 2 cut(s) 268, 2788
LguI GCTCTTC 4 cut(s) 1929, 3978, 4071, 5535
MabI ACCWGGT 1 cut(s) 4102
MaeII ACGT 2 cut(s) 1215, 2396
MfeI CAATTG 5 cut(s) 117, 954, 2328, 3033, 5388
MflI RGATCY 5 cut(s) 20, 974, 1433, 2810, 3637
MhlI GDGCHC 8 cut(s) 1145, 1279, 2308, 3249, 4066, 4459, 5720, 5757
MlsI TGGCCA 2 cut(s) 1844, 4392
MluI ACGCGT 1 cut(s) 2668
MluNI TGGCCA 2 cut(s) 1844, 4392
MmeI TCCRAC 6 cut(s) 783, 1572, 2082, 4207, 4714, 4841
Mox20I TGGCCA 2 cut(s) 1844, 4392
Mph1103I ATGCAT 3 cut(s) 6, 462, 5034
MroI TCCGGA 1 cut(s) 4330
MroXI GAANNNNTTC 2 cut(s) 1017, 4177
MscI TGGCCA 2 cut(s) 1844, 4392
MslI CAYNNNNRTG 5 cut(s) 9, 2618, 3798, 4595, 5085
Msp20I TGGCCA 2 cut(s) 1844, 4392
MspA1I CMGCKG 7 cut(s) 1139, 1289, 2012, 2143, 4627, 4963, 5336
MspI CCGG 7 cut(s) 437, 689, 731, 4331, 4808, 5105, 5624
MunI CAATTG 5 cut(s) 117, 954, 2328, 3033, 5388
Mva1269I GAATGC 8 cut(s) 235, 1237, 2005, 2734, 3398, 3498, 4002, 5251
MvnI CGCG 4 cut(s) 106, 663, 2670, 4299
NciI CCSGG 1 cut(s) 5106
NcoI CCATGG 2 cut(s) 54, 3266
NdeI CATATG 1 cut(s) 5461
NmeAIII GCCGAG 1 cut(s) 134
NruI TCGCGA 1 cut(s) 4299
NsbI TGCGCA 3 cut(s) 2675, 4595, 5829
NsiI ATGCAT 3 cut(s) 6, 462, 5034
NspI RCATGY 3 cut(s) 2374, 2469, 4688
NspV TTCGAA 3 cut(s) 2493, 4093, 5000
OliI CACNNNNGTG 1 cut(s) 3798
PaeI GCATGC 1 cut(s) 2374
PaqCI CACCTGC 4 cut(s) 2941, 4623, 4848, 5401
PceI AGGCCT 1 cut(s) 2632
PciSI GCTCTTC 4 cut(s) 1929, 3978, 4071, 5535
PcsI WCGNNNNNNNCGW 1 cut(s) 4090
PctI GAATGC 8 cut(s) 235, 1237, 2005, 2734, 3398, 3498, 4002, 5251
PdmI GAANNNNTTC 2 cut(s) 1017, 4177
PflFI GACNNNGTC 3 cut(s) 344, 3124, 4567
PflMI CCANNNNNTGG 4 cut(s) 3122, 3257, 4412, 4850
PfoI TCCNGGA 3 cut(s) 4069, 4273, 5104
PinAI ACCGGT 2 cut(s) 688, 5623
Ppu21I YACGTR 1 cut(s) 2397
PpuMI RGGWCCY 1 cut(s) 2016
PshAI GACNNNNGTC 1 cut(s) 1338
PshBI ATTAAT 1 cut(s) 1350
PsiI TTATAA 1 cut(s) 2541
Psp5II RGGWCCY 1 cut(s) 2016
PspFI CCCAGC 4 cut(s) 2012, 4043, 4479, 4765
PspPPI RGGWCCY 1 cut(s) 2016
PstNI CAGNNNCTG 8 cut(s) 23, 239, 893, 1511, 2576, 3257, 5851, 5986
PsuI RGATCY 5 cut(s) 20, 974, 1433, 2810, 3637
PsyI GACNNNGTC 3 cut(s) 344, 3124, 4567
PvuII CAGCTG 3 cut(s) 1289, 2012, 5336
RruI TCGCGA 1 cut(s) 4299
RseI CAYNNNNRTG 5 cut(s) 9, 2618, 3798, 4595, 5085
SalI GTCGAC 1 cut(s) 360
SapI GCTCTTC 4 cut(s) 1929, 3978, 4071, 5535
ScaI AGTACT 2 cut(s) 3355, 5560
SduI GDGCHC 8 cut(s) 1145, 1279, 2308, 3249, 4066, 4459, 5720, 5757
SexAI ACCWGGT 1 cut(s) 4102
SfcI CTRYAG 2 cut(s) 2317, 3654
SfuI TTCGAA 3 cut(s) 2493, 4093, 5000
SmiI ATTTAAAT 2 cut(s) 1089, 2073
SmiMI CAYNNNNRTG 5 cut(s) 9, 2618, 3798, 4595, 5085
SmlI CTYRAG 6 cut(s) 129, 191, 1823, 3563, 5319, 5697
SmoI CTYRAG 6 cut(s) 129, 191, 1823, 3563, 5319, 5697
SpeI ACTAGT 2 cut(s) 1181, 3797
SphI GCATGC 1 cut(s) 2374
SseBI AGGCCT 1 cut(s) 2632
SspI AATATT 1 cut(s) 840
StuI AGGCCT 1 cut(s) 2632
SwaI ATTTAAAT 2 cut(s) 1089, 2073
TaiI ACGT 2 cut(s) 1218, 2399
TaqII GACCGA 1 cut(s) 1058
TatI WGTACW 6 cut(s) 622, 1496, 3304, 3353, 4784, 5558
TauI GCSGC 3 cut(s) 4318, 4684, 4966
TspGWI ACGGA 3 cut(s) 545, 1759, 3229
Tth111I GACNNNGTC 3 cut(s) 344, 3124, 4567
Van91I CCANNNNNTGG 4 cut(s) 3122, 3257, 4412, 4850
VneI GTGCAC 1 cut(s) 4455
VspI ATTAAT 1 cut(s) 1350
XapI RAATTY 9 cut(s) 185, 221, 948, 2074, 3411, 4279, 5494, 5686, 5731
XbaI TCTAGA 1 cut(s) 3088
XceI RCATGY 3 cut(s) 2374, 2469, 4688
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XmaJI CCTAGG 2 cut(s) 776, 2555
XmiI GTMKAC 3 cut(s) 254, 361, 1692
XmnI GAANNNNTTC 2 cut(s) 1017, 4177
ZrmI AGTACT 2 cut(s) 3355, 5560
Zsp2I ATGCAT 3 cut(s) 6, 462, 5034
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.