pycom02g04100

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013585
Physical Location & Seq
Forward (+)
2706230 .. 2709021
2792 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1665 bp
ATGATTTCATCACCATATCCGCCAATGGGGACCGAAGTGGTGGTCACGACGGACCGAGTCCAGAAGTTCTTCGAGGACCTGGAGGCGCAGAAGGCGATTCTCTCCACCTCTACCAAGCTTTTCACAACCCTCTCCGACCACTTCGATTCCCTCCAGAAATCCATTTCCGAGAAATCCCATTCTCTCGAATCCAAAATCCAATCCCTGGAATCCCGTTCCCGCGAAACCTTACAATCCCTCGACCACCGCGAGGCCTCCATCCCGGAGCGCGAGTACTCCGCCGCTGCTCGAATCGAGGAGCAGAAAGCCGCGGCCTTGGCCGAGGTGGAGAAGAATGTTTTTGGGGATTTGGATTTGGCGGAGACCCTGAAATCGTTTTGCAGGAAAATGGACTCCCAGGGTTTGGTGAAGTTCATCGTGTCGAAGCGGAAGGAATCGATATCGCTCCGGGCGGAGATTGTACAGGCGATTTCGGAGGCGGTGGACCCGCTAAGATTGGTGCTTGATGCATTGGAAGATTTTTTGGATTCTAAAGCCAAGAAATTCGGGGTTACCGATAAGAGGTGGGCGTGCGGGCTGTTAGTTCAGGCTCTGTTTCCGGAGGGTAAGCCCAATGCTGGCGCGAAGGGGCCGGAGTTTTCTAGGAGCATGGTGGAGAGAGCGGCGGGGATTGCAGATATGTGGAAGGCGCAGATGGATGGAGATTCGAACGGTGGTGGCGGGACATTGGGGGCTGCGGAGGCGGTGATGTTTGTGCAAATGCTCGTTGGGTTTCGATTGAAGGAGAAGTTCGGTGAGGAGTTCTTTAGGAAGTTGGTAATGGAGCACGCAGCGAGGAGGGATATGGCAAAGCTAGCAAGGGCTCTAGAATTCGGAGAGAAAATGGGAGATGTCATTGATGAGTTAGTGAAGAATGGCAAGGAGATAGAGGCTGTATATTTTGCTTCTGAGTCTGATTTGACCGAGAGATTTCCTCCTGTTTCTCTGCTCAAGTCGTATCTCAAGAACTCCAGAAAGAATGCTGCGACCATATTGAAGAATGGCAATAATAGTGTGGCTGCTACTGAGGAATCGAGCACATTTGAGTTGAATTCCATCAAAGCAATCATCAAATGTGTAGAAGACCACAAGCTTGAATCAGAGTTTTCTCTTGAGAACTTAAGAAAGCGGGCCACTCATCTGGAGAAAGCAAGGACAGAAAGGAAGAAGAGATCAGCATCTTCCAGCAGGCCTCATAATAATAAGCGAGCCTACGGTGGTAGTGGAGGTGGTGGCAGCGGCAGTGGCGGGGGTGGTGGCCGAGGGGTTGGACAGTCTTCTTCCCGCCCTGCCAAAGCTGCCAAGTTTTCCAATGCATACCCCTCTTTCACCCGCAGGAACCCAAATCCTCTTCCACAGCATAGTCCTGCAACAAGATACTCTGGTCCTTATAATTACCCGAGCCAAAGTGTGTACGATGGTCCAACAGTAGCATCATATGGATCCACATACGGAGCTCCCCCTGCTCAAAGCCCCGCTGCTCTTCCGTCGCAACACTACTCCCTCTCTGGGGACAGCTTGGGTGCTGCCGGCTTCCGAAGTAGTGGTGCTTATGGTGGCCAGACCAACTACGGGTACGCAAGTGCTGCTCCACCAGCGTACCAGCCTCCTTCGTATCCGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

555

Amino Acids

60.08

Weight (kDa)

9.01

Isoelectric Point (pI)

55.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 119 - 404 3.2e-95 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013585)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g05070 FvH4_1g05070 FvH4_1g05070
malus_domestica MD02G1048900.v1.1 MD15G1187200.v1.1
prunus_persica Prupe.7G229200_v2.0.a1
pyrus_communis pycom02g04100 pycom15g16770
rosa_chinensis RchiOBHm_Chr2g0090711
rosa_laevigata RLG00000016136
rosa_multiflora Rmu_ssc0000367.1_g000001
rosa_roxburghii Rroxscaffold_2G00150890
rosa_rugosa Rorug02G0009900
rosa_samantha Rh2AG055900 Rh2BG054300 Rh2CG056600 Rh2DG055400
rosa_wichuraiana Rw2G004670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1431
AccB7I CCANNNNNTGG 2 cut(s) 205, 403
AccBSI CCGCTC 1 cut(s) 662
AccII CGCG 5 cut(s) 222, 249, 270, 311, 623
AccIII TCCGGA 1 cut(s) 598
AclWI GGATC 2 cut(s) 1476, 1489
AcoI YGGCCR 3 cut(s) 318, 1297, 1597
AcsI RAATTY 3 cut(s) 542, 869, 1090
AfaI GTAC 5 cut(s) 275, 462, 1454, 1616, 1640
AfiI CCNNNNNNNGG 9 cut(s) 26, 205, 250, 403, 561, 617, 1548, 1549, 1611
AflII CTTAAG 1 cut(s) 1159
AgsI TTSAA 4 cut(s) 781, 1036, 1090, 1136
AjnI CCWGG 3 cut(s) 78, 204, 396
AluBI AGCT 6 cut(s) 118, 853, 1132, 1337, 1496, 1557
AluI AGCT 6 cut(s) 118, 853, 1132, 1337, 1496, 1557
Alw21I GWGCWC 3 cut(s) 828, 1079, 1498
Alw26I GTCTC 1 cut(s) 356
AlwI GGATC 2 cut(s) 1476, 1489
AlwNI CAGNNNCTG 1 cut(s) 592
Ama87I CYCGRG 1 cut(s) 1438
Aor13HI TCCGGA 1 cut(s) 598
AoxI GGCC 8 cut(s) 252, 312, 318, 629, 1170, 1229, 1297, 1597
ApoI RAATTY 3 cut(s) 542, 869, 1090
ArsI GACNNNNNNTTYG 2 cut(s) 27, 59
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 4 cut(s) 88, 270, 623, 691
AspS9I GGNCC 8 cut(s) 30, 52, 76, 484, 629, 1170, 1424, 1460
AsuC2I CCSGG 2 cut(s) 263, 449
AsuHPI GGTGA 5 cut(s) 3, 418, 757, 806, 1360
AsuII TTCGAA 1 cut(s) 707
AsuNHI GCTAGC 1 cut(s) 853
AvaI CYCGRG 1 cut(s) 1438
AvaII GGWCC 6 cut(s) 30, 52, 76, 484, 1424, 1460
BaeI ACNNNNGTAYC 6 cut(s) 1408, 1441, 1606, 1622, 1639, 1655
BalI TGGCCA 1 cut(s) 1599
BamHI GGATCC 1 cut(s) 1481
BanII GRGCYC 2 cut(s) 865, 1498
BbsI GAAGAC 2 cut(s) 1128, 1308
Bbv12I GWGCWC 3 cut(s) 828, 1079, 1498
BccI CCATC 5 cut(s) 266, 688, 692, 1103, 1451
BciT130I CCWGG 3 cut(s) 80, 206, 398
BciVI GTATCC 1 cut(s) 1665
BcnI CCSGG 2 cut(s) 263, 449
BcoDI GTCTC 1 cut(s) 356
BfaI CTAG 3 cut(s) 642, 854, 866
BfrI CTTAAG 1 cut(s) 1159
BfuI GTATCC 1 cut(s) 1665
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 5 cut(s) 80, 206, 263, 398, 449
Bme18I GGWCC 6 cut(s) 30, 52, 76, 484, 1424, 1460
BmeT110I CYCGRG 1 cut(s) 1438
BmgT120I GGNCC 8 cut(s) 30, 52, 76, 484, 629, 1170, 1424, 1460
BmiI GGNNCC 5 cut(s) 31, 486, 630, 1379, 1483
BmrFI CCNGG 5 cut(s) 80, 206, 263, 398, 449
BmsI GCATC 3 cut(s) 496, 1226, 1481
BmtI GCTAGC 1 cut(s) 857
BpiI GAAGAC 2 cut(s) 1128, 1308
BplI GAGNNNNNCTC 2 cut(s) 958, 990
BpmI CTGGAG 4 cut(s) 101, 137, 994, 1202
Bpu14I TTCGAA 1 cut(s) 707
BpuEI CTTGAG 3 cut(s) 974, 986, 1172
BpuMI CCSGG 2 cut(s) 263, 449
Bsa29I ATCGAT 1 cut(s) 437
BsaBI GATNNNNATC 1 cut(s) 1216
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 7 cut(s) 204, 309, 315, 321, 396, 397, 1300
BsaWI WCCGGW 1 cut(s) 598
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 9 cut(s) 26, 205, 250, 403, 561, 617, 1548, 1549, 1611
Bse118I RCCGGY 1 cut(s) 1568
Bse8I GATNNNNATC 1 cut(s) 1216
BseAI TCCGGA 1 cut(s) 598
BseBI CCWGG 3 cut(s) 80, 206, 398
BseCI ATCGAT 1 cut(s) 437
BseDI CCNNGG 7 cut(s) 204, 309, 315, 321, 396, 397, 1300
BseGI GGATG 2 cut(s) 258, 703
BseJI GATNNNNATC 1 cut(s) 1216
BseLI CCNNNNNNNGG 9 cut(s) 26, 205, 250, 403, 561, 617, 1548, 1549, 1611
BseMII CTCAG 2 cut(s) 939, 1056
BseRI GAGGAG 3 cut(s) 311, 812, 850
Bsh1236I CGCG 5 cut(s) 222, 249, 270, 311, 623
BshFI GGCC 8 cut(s) 254, 314, 320, 631, 1172, 1231, 1299, 1599
BshVI ATCGAT 1 cut(s) 437
BsiHKAI GWGCWC 3 cut(s) 828, 1079, 1498
BsiHKCI CYCGRG 1 cut(s) 1438
BsiSI CCGG 5 cut(s) 263, 448, 599, 632, 1569
BslFI GGGAC 3 cut(s) 43, 736, 1565
BslI CCNNNNNNNGG 9 cut(s) 26, 205, 250, 403, 561, 617, 1548, 1549, 1611
BsmAI GTCTC 1 cut(s) 356
BsmFI GGGAC 3 cut(s) 43, 736, 1565
BsmI GAATGC 1 cut(s) 1024
BsnI GGCC 8 cut(s) 254, 314, 320, 631, 1172, 1231, 1299, 1599
Bso31I GGTCTC 1 cut(s) 356
BsoBI CYCGRG 1 cut(s) 1438
Bsp119I TTCGAA 1 cut(s) 707
Bsp1286I GDGCHC 4 cut(s) 828, 865, 1079, 1498
Bsp13I TCCGGA 1 cut(s) 598
Bsp1407I TGTACA 1 cut(s) 460
Bsp143I GATC 2 cut(s) 1211, 1481
BspANI GGCC 8 cut(s) 254, 314, 320, 631, 1172, 1231, 1299, 1599
BspCNI CTCAG 2 cut(s) 940, 1057
BspDI ATCGAT 1 cut(s) 437
BspEI TCCGGA 1 cut(s) 598
BspFNI CGCG 5 cut(s) 222, 249, 270, 311, 623
BspLI GGNNCC 5 cut(s) 31, 486, 630, 1379, 1483
BspOI GCTAGC 1 cut(s) 857
BspPI GGATC 2 cut(s) 1476, 1489
BspQI GCTCTTC 1 cut(s) 1527
BspT104I TTCGAA 1 cut(s) 707
BspTI CTTAAG 1 cut(s) 1159
BspTNI GGTCTC 1 cut(s) 356
BsrBI CCGCTC 1 cut(s) 662
BsrFI RCCGGY 1 cut(s) 1568
BsrGI TGTACA 1 cut(s) 460
BssAI RCCGGY 1 cut(s) 1568
BssECI CCNNGG 7 cut(s) 204, 309, 315, 321, 396, 397, 1300
BssMI GATC 2 cut(s) 1211, 1481
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 3 cut(s) 80, 206, 398
Bst4CI ACNGT 4 cut(s) 713, 1256, 1314, 1468
Bst6I CTCTTC 3 cut(s) 1202, 1395, 1527
BstAFI CTTAAG 1 cut(s) 1159
BstAPI GCANNNNNTGC 1 cut(s) 1625
BstAUI TGTACA 1 cut(s) 460
BstBI TTCGAA 1 cut(s) 707
BstC8I GCNNGC 9 cut(s) 571, 575, 619, 828, 855, 1170, 1229, 1248, 1570
BstDEI CTNAG 3 cut(s) 491, 948, 1065
BstDSI CCRYGG 1 cut(s) 309
BstEII GGTNACC 1 cut(s) 550
BstF5I GGATG 2 cut(s) 258, 703
BstFNI CGCG 5 cut(s) 222, 249, 270, 311, 623
BstHHI GCGC 4 cut(s) 88, 270, 623, 691
BstKTI GATC 2 cut(s) 1214, 1484
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 2 cut(s) 1211, 1481
BstNI CCWGG 3 cut(s) 80, 206, 398
BstPI GGTNACC 1 cut(s) 550
BstSCI CCNGG 5 cut(s) 78, 204, 261, 396, 447
BstUI CGCG 5 cut(s) 222, 249, 270, 311, 623
BstV2I GAAGAC 2 cut(s) 1128, 1308
BstX2I RGATCY 1 cut(s) 1481
BstXI CCANNNNNNTGG 1 cut(s) 1180
BstYI RGATCY 1 cut(s) 1481
Bsu15I ATCGAT 1 cut(s) 437
BsuI GTATCC 1 cut(s) 1665
BsuRI GGCC 8 cut(s) 254, 314, 320, 631, 1172, 1231, 1299, 1599
BsuTUI ATCGAT 1 cut(s) 437
BtgI CCRYGG 1 cut(s) 309
BtsCI GGATG 2 cut(s) 258, 703
BtsI GCAGTG 1 cut(s) 1288
BtsIMutI CAGTG 1 cut(s) 1288
Cac8I GCNNGC 9 cut(s) 571, 575, 619, 828, 855, 1170, 1229, 1248, 1570
CaiI CAGNNNCTG 1 cut(s) 592
CfoI GCGC 4 cut(s) 88, 270, 623, 691
Cfr10I RCCGGY 1 cut(s) 1568
Cfr13I GGNCC 8 cut(s) 30, 52, 76, 484, 629, 1170, 1424, 1460
Cfr42I CCGCGG 1 cut(s) 312
ClaI ATCGAT 1 cut(s) 437
CpoI CGGWCCG 1 cut(s) 52
Csp6I GTAC 5 cut(s) 274, 461, 1453, 1615, 1639
CspI CGGWCCG 1 cut(s) 52
CviAII CATG 1 cut(s) 649
CviQI GTAC 5 cut(s) 274, 461, 1453, 1615, 1639
DdeI CTNAG 3 cut(s) 491, 948, 1065
DpnI GATC 2 cut(s) 1213, 1483
DpnII GATC 2 cut(s) 1211, 1481
EaeI YGGCCR 3 cut(s) 318, 1297, 1597
Eam1104I CTCTTC 3 cut(s) 1202, 1395, 1527
EarI CTCTTC 3 cut(s) 1202, 1395, 1527
EciI GGCGGA 5 cut(s) 9, 268, 374, 467, 1647
Ecl136II GAGCTC 1 cut(s) 1496
Eco130I CCWWGG 1 cut(s) 315
Eco147I AGGCCT 2 cut(s) 254, 1231
Eco24I GRGCYC 2 cut(s) 865, 1498
Eco31I GGTCTC 1 cut(s) 356
Eco32I GATATC 1 cut(s) 441
Eco47I GGWCC 6 cut(s) 30, 52, 76, 484, 1424, 1460
Eco53kI GAGCTC 1 cut(s) 1496
Eco88I CYCGRG 1 cut(s) 1438
Eco91I GGTNACC 1 cut(s) 550
EcoICRI GAGCTC 1 cut(s) 1496
EcoO109I RGGNCCY 1 cut(s) 76
EcoO65I GGTNACC 1 cut(s) 550
EcoRI GAATTC 2 cut(s) 869, 1090
EcoRII CCWGG 3 cut(s) 78, 204, 396
EcoRV GATATC 1 cut(s) 441
EcoT14I CCWWGG 1 cut(s) 315
EcoT22I ATGCAT 2 cut(s) 511, 1357
EcoT38I GRGCYC 2 cut(s) 865, 1498
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 1 cut(s) 652
FaqI GGGAC 3 cut(s) 43, 736, 1565
FatI CATG 1 cut(s) 648
FauNDI CATATG 1 cut(s) 1477
FokI GGATG 2 cut(s) 245, 710
FriOI GRGCYC 2 cut(s) 865, 1498
FspBI CTAG 3 cut(s) 642, 854, 866
GlaI GCGC 4 cut(s) 87, 269, 622, 690
GsuI CTGGAG 4 cut(s) 101, 137, 994, 1202
HaeIII GGCC 8 cut(s) 254, 314, 320, 631, 1172, 1231, 1299, 1599
HapII CCGG 5 cut(s) 263, 448, 599, 632, 1569
HhaI GCGC 4 cut(s) 88, 270, 623, 691
Hin1II CATG 1 cut(s) 652
Hin6I GCGC 4 cut(s) 86, 268, 621, 689
HinP1I GCGC 4 cut(s) 86, 268, 621, 689
HindIII AAGCTT 2 cut(s) 116, 1130
HpaII CCGG 5 cut(s) 263, 448, 599, 632, 1569
HphI GGTGA 5 cut(s) 3, 418, 757, 806, 1360
Hpy166II GTNNAC 2 cut(s) 484, 1453
Hpy188I TCNGA 8 cut(s) 136, 169, 475, 875, 949, 955, 1141, 1577
Hpy8I GTNNAC 2 cut(s) 484, 1453
Hpy99I CGWCG 2 cut(s) 52, 1531
HpyAV CCTTC 6 cut(s) 85, 424, 619, 679, 775, 1659
HpyCH4III ACNGT 4 cut(s) 713, 1256, 1314, 1468
HpyCH4V TGCA 6 cut(s) 381, 509, 674, 757, 1355, 1409
HpyF3I CTNAG 3 cut(s) 491, 948, 1065
Hsp92II CATG 1 cut(s) 652
HspAI GCGC 4 cut(s) 86, 268, 621, 689
Kpn2I TCCGGA 1 cut(s) 598
KroI GCCGGC 1 cut(s) 1568
KroNI GCCGGC 1 cut(s) 1570
KspI CCGCGG 1 cut(s) 312
Kzo9I GATC 2 cut(s) 1211, 1481
LguI GCTCTTC 1 cut(s) 1527
LmnI GCTCC 8 cut(s) 265, 298, 450, 645, 823, 1493, 1501, 1633
LweI GCATC 3 cut(s) 496, 1226, 1481
MaeI CTAG 3 cut(s) 642, 854, 866
MaeIII GTNAC 2 cut(s) 43, 550
MalI GATC 2 cut(s) 1213, 1483
MbiI CCGCTC 1 cut(s) 662
MboI GATC 2 cut(s) 1211, 1481
MflI RGATCY 1 cut(s) 1481
MhlI GDGCHC 4 cut(s) 828, 865, 1079, 1498
MlsI TGGCCA 1 cut(s) 1599
MluCI AATT 4 cut(s) 542, 869, 1090, 1432
MluNI TGGCCA 1 cut(s) 1599
MlyI GAGTC 3 cut(s) 66, 386, 959
MmeI TCCRAC 3 cut(s) 159, 1288, 1487
Mox20I TGGCCA 1 cut(s) 1599
Mph1103I ATGCAT 2 cut(s) 511, 1357
MroI TCCGGA 1 cut(s) 598
MroNI GCCGGC 1 cut(s) 1568
MroXI GAANNNNTTC 1 cut(s) 68
MscI TGGCCA 1 cut(s) 1599
MseI TTAA 1 cut(s) 1160
Msp20I TGGCCA 1 cut(s) 1599
MspA1I CMGCKG 4 cut(s) 284, 311, 1278, 1517
MspCI CTTAAG 1 cut(s) 1159
MspI CCGG 5 cut(s) 263, 448, 599, 632, 1569
MspR9I CCNGG 5 cut(s) 80, 206, 263, 398, 449
Mva1269I GAATGC 1 cut(s) 1024
MvaI CCWGG 3 cut(s) 80, 206, 398
MvnI CGCG 5 cut(s) 222, 249, 270, 311, 623
NaeI GCCGGC 1 cut(s) 1570
NciI CCSGG 2 cut(s) 263, 449
NdeI CATATG 1 cut(s) 1477
NdeII GATC 2 cut(s) 1211, 1481
NgoMIV GCCGGC 1 cut(s) 1568
NheI GCTAGC 1 cut(s) 853
NlaIII CATG 1 cut(s) 652
NlaIV GGNNCC 5 cut(s) 31, 486, 630, 1379, 1483
NmeAIII GCCGAG 2 cut(s) 346, 1325
NmuCI GTSAC 1 cut(s) 43
NsiI ATGCAT 2 cut(s) 511, 1357
NspV TTCGAA 1 cut(s) 707
PasI CCCWGGG 1 cut(s) 397
PceI AGGCCT 2 cut(s) 254, 1231
PciSI GCTCTTC 1 cut(s) 1527
PcsI WCGNNNNNNNCGW 2 cut(s) 246, 552
PctI GAATGC 1 cut(s) 1024
PdiI GCCGGC 1 cut(s) 1570
PdmI GAANNNNTTC 1 cut(s) 68
PflFI GACNNNGTC 1 cut(s) 56
PflMI CCANNNNNTGG 2 cut(s) 205, 403
PfoI TCCNGGA 1 cut(s) 261
PleI GAGTC 3 cut(s) 65, 386, 958
PpsI GAGTC 3 cut(s) 65, 386, 958
PpuMI RGGWCCY 1 cut(s) 76
PsiI TTATAA 1 cut(s) 1431
Psp124BI GAGCTC 1 cut(s) 1498
Psp5II RGGWCCY 1 cut(s) 76
Psp6I CCWGG 3 cut(s) 78, 204, 396
PspEI GGTNACC 1 cut(s) 550
PspGI CCWGG 3 cut(s) 78, 204, 396
PspN4I GGNNCC 5 cut(s) 31, 486, 630, 1379, 1483
PspPI GGNCC 8 cut(s) 30, 52, 76, 484, 629, 1170, 1424, 1460
PspPPI RGGWCCY 1 cut(s) 76
PstNI CAGNNNCTG 1 cut(s) 592
PsuI RGATCY 1 cut(s) 1481
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 5 cut(s) 275, 462, 1454, 1616, 1640
RsaNI GTAC 5 cut(s) 274, 461, 1453, 1615, 1639
Rsr2I CGGWCCG 1 cut(s) 52
RsrII CGGWCCG 1 cut(s) 52
SacI GAGCTC 1 cut(s) 1498
SacII CCGCGG 1 cut(s) 312
SapI GCTCTTC 1 cut(s) 1527
SaqAI TTAA 1 cut(s) 1160
Sau3AI GATC 2 cut(s) 1211, 1481
Sau96I GGNCC 8 cut(s) 30, 52, 76, 484, 629, 1170, 1424, 1460
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 3 cut(s) 66, 386, 959
ScrFI CCNGG 5 cut(s) 80, 206, 263, 398, 449
SduI GDGCHC 4 cut(s) 828, 865, 1079, 1498
SfaNI GCATC 3 cut(s) 496, 1226, 1481
Sfr303I CCGCGG 1 cut(s) 312
SfuI TTCGAA 1 cut(s) 707
SgrBI CCGCGG 1 cut(s) 312
SinI GGWCC 6 cut(s) 30, 52, 76, 484, 1424, 1460
SmlI CTYRAG 4 cut(s) 989, 1001, 1151, 1159
SmoI CTYRAG 4 cut(s) 989, 1001, 1151, 1159
Sse9I AATT 4 cut(s) 542, 869, 1090, 1432
SseBI AGGCCT 2 cut(s) 254, 1231
SspMI CTAG 3 cut(s) 642, 854, 866
SstI GAGCTC 1 cut(s) 1498
StuI AGGCCT 2 cut(s) 254, 1231
StyD4I CCNGG 5 cut(s) 78, 204, 261, 396, 447
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 4 cut(s) 713, 1256, 1314, 1468
TaqII GACCGA 3 cut(s) 47, 69, 977
TasI AATT 4 cut(s) 542, 869, 1090, 1432
TatI WGTACW 2 cut(s) 273, 460
TauI GCSGC 5 cut(s) 284, 311, 314, 665, 1281
Tru1I TTAA 1 cut(s) 1160
Tru9I TTAA 1 cut(s) 1160
TscAI CASTG 1 cut(s) 1288
TseFI GTSAC 1 cut(s) 43
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 1 cut(s) 403
TspGWI ACGGA 3 cut(s) 65, 1506, 1515
TspRI CASTG 1 cut(s) 1288
Tth111I GACNNNGTC 1 cut(s) 56
Van91I CCANNNNNTGG 2 cut(s) 205, 403
Vha464I CTTAAG 1 cut(s) 1159
VpaK11BI GGWCC 6 cut(s) 30, 52, 76, 484, 1424, 1460
XapI RAATTY 3 cut(s) 542, 869, 1090
XbaI TCTAGA 1 cut(s) 865
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 3 cut(s) 642, 854, 866
ZrmI AGTACT 1 cut(s) 275
Zsp2I ATGCAT 2 cut(s) 511, 1357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.