Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0011352

Physical Location & Seq

Genomic Coordinates Forward (+)

3592699 .. 3595591

Gene Structure 2893 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g05370.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGGGAAGTGGGTTCTTCCGGCTTGTCATTGCCACTTCAGTGCTGCTTACACTTTTGGATGTTTGCCGAGGAAGTACCATTGGAATTTGCTACGGTAGAAATGCTGATGATCTTCCGACGCCTGACAAAGCATCGCAGCTTGTGCAGCTCCATAACATTAAATATGTTAGGATTTACGACTCCAACATACAAGTTCTCAAGGCCTTTGCAAACACTGGCGTTGAACTTATGATTGGGGTTCCAAATTCAGATTTGTTGGCGTTTTCACAGTTCCAATCCAATGTAGACACCTGGCTCAAGAACAGTATCCTGCCTTACTACCCAGGGACGAAGATCGCATACATAACGGTTGGAGCTGAAGTTACTGAGAGTTCCAGTAATTTCTCTGTCCAAGTAATGCCAGCCATGAGAAATGTCTTCACAGCCTTAAAAAAAGTTGGTCTGTCTAAGAAGATCAAATTATCAAGCACACATTCCCTCGGTGTTCTGTCCCGATCATTTCCTCCCTCAGCAGGGGCTTTTAATAGCAGCCATGCGTCTTTCCTGAAACCGCTGTTGGAATTCCTCGCTGAAAACCAGTCACCCTTCATGGTTGATATTTATCCATACTATGCTTACAGAGATTCTCCAAGCAATGTCACTTTGGATTACGCTCTATTTGATGCATCGTCAGAAGTTATTGATCCAAACACTGGTTTGATATACACTAACATGCTCGATGCTCAGATTGACGCTATATATTACGCGCTGATGGCCCTGAATTTCAAAACAATCAAAGTTATGGTCACTGAGACGGGCTGGCCTTCAAAAGGATCGCCTAAAGAGACAGCTGCAACTCCTGATAATGCACAAACTTACAATACCAATTTGATTCGCCATGTCATTAACAACACTGGCACTCCTGCAAAGCCGGGAGAGAGGCTGGATGTGTACATCTTCTCTTTGTTCAACGAAAATAGGAAGCCAGGGTTGGAATCTGAGAGGAACTGGGGGCTATTTTACCCAGACCAGACTAGCGTCTACAACCTGGATTTCACTGGAAAAAGTTCGGTGGATATGATTACAGAGACTAATGTTACCAGTTCAAATGGAACGACACCGACATGGTGCATTGCTTCGAGTAAGGCTTCTGAACTCGACTTGCAGAATGCCTTGGATTGGGCTTGTGGTCCTGGGAATGTGGACTGTAGAGCTATTCAGCCTAGCCAGCCTTGTTTCGAGCCAGATAATACCGTTTCTCATGCGTCATACGCATTCAACAGTTATTACCAGCAAAATGGGGCTACTGATATTGCTTGCAGTTTCGGAGGAACAGGGTTCAAGGTCGATAAGAACCCAAGCTATGACAACTGCATCTATATGACTACTGGGAGCAACAAAACCGTGCCAAGTAATACGACTGCAATATCTTCTACTTCTTCCTCTTCCACAATGAGCGGAGTTCCGGTGTGGATTTGTAGTTGCTTTCTGGTGACTTTCATGTCGTTTCTTTCCCCCGATACCAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001871 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0016787 GO:0016798 GO:0030246 GO:0030247 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_17 X8

Protein Analysis

503

Amino Acids

54.88

Weight (kDa)

5.35

Isoelectric Point (pI)

33.79

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011352)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56590
fragaria_vesca	FvH4_1g06440
malus_domestica	MD02G1067800.v1.1 MD15G1199400.v1.1
prunus_persica	Prupe.7G217800_v2.0.a1 Prupe.7G217800_v2.0.a1
pyrus_communis	pycom02g05370 pycom15g17690
rosa_chinensis	RchiOBHm_Chr2g0092181
rosa_laevigata	RLG00000016283
rosa_multiflora	Rmu_sc0000524.1_g000021 Rmu_sc0030695.1_g000001 Rmu_sc0030696.1_g000001
rosa_roxburghii	Rroxscaffold_2G00149400
rosa_rugosa	Rorug02G0023500 Rorug02G0023600 Rorug02G0023600
rosa_samantha	Rh2AG069200 Rh2CG069900
rosa_wichuraiana	Rw2G006300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1480
AccB7I	CCANNNNNTGG	1 cut(s)	692
AccBSI	CCGCTC	1 cut(s)	1437
AccI	GTMKAC	2 cut(s)	285, 1020
AccII	CGCG	1 cut(s)	746
AciI	CCGC	2 cut(s)	551, 1437
AclWI	GGATC	2 cut(s)	677, 820
AcsI	RAATTY	4 cut(s)	84, 244, 560, 760
AcuI	CTGAAG	2 cut(s)	21, 378
AcyI	GRCGYC	1 cut(s)	119
AfaI	GTAC	2 cut(s)	76, 932
AfiI	CCNNNNNNNGG	5 cut(s)	512, 513, 692, 809, 1158
AgsI	TTSAA	7 cut(s)	224, 766, 807, 949, 1086, 1258, 1321
AjnI	CCWGG	5 cut(s)	290, 322, 964, 1026, 1171
AjuI	GAANNNNNNNTTGG	6 cut(s)	216, 248, 539, 571, 953, 985
AleI	CACNNNNGTG	1 cut(s)	38
AluBI	AGCT	6 cut(s)	139, 148, 356, 830, 1193, 1341
AluI	AGCT	6 cut(s)	139, 148, 356, 830, 1193, 1341
Alw26I	GTCTC	3 cut(s)	785, 818, 1061
AlwI	GGATC	2 cut(s)	677, 820
AoxI	GGCC	3 cut(s)	201, 753, 800
ApeKI	GCWGC	5 cut(s)	43, 136, 145, 528, 830
ApoI	RAATTY	4 cut(s)	84, 244, 560, 760
ArsI	GACNNNNNNTTYG	2 cut(s)	768, 800
Asp700I	GAANNNNTTC	1 cut(s)	1045
AspLEI	GCGC	1 cut(s)	748
AspS9I	GGNCC	2 cut(s)	754, 1169
AsuC2I	CCSGG	1 cut(s)	912
AsuHPI	GGTGA	2 cut(s)	573, 1483
AvaII	GGWCC	1 cut(s)	1169
BarI	GAAGNNNNNNTAC	2 cut(s)	323, 355
BbsI	GAAGAC	1 cut(s)	409
BbvCI	CCTCAGC	1 cut(s)	508
BbvI	GCAGC	5 cut(s)	30, 148, 157, 540, 817
BccI	CCATC	1 cut(s)	745
BciT130I	CCWGG	5 cut(s)	292, 324, 966, 1028, 1173
BciVI	GTATCC	1 cut(s)	317
BcnI	CCSGG	1 cut(s)	912
BcoDI	GTCTC	3 cut(s)	785, 818, 1061
BfaI	CTAG	2 cut(s)	1014, 1203
BfmI	CTRYAG	1 cut(s)	1186
BfuI	GTATCC	1 cut(s)	317
BisI	GCNGC	5 cut(s)	44, 137, 146, 529, 831
BlsI	GCNGC	5 cut(s)	45, 138, 147, 530, 832
Bme1390I	CCNGG	6 cut(s)	292, 324, 912, 966, 1028, 1173
Bme18I	GGWCC	1 cut(s)	1169
BmgT120I	GGNCC	2 cut(s)	754, 1169
BmiI	GGNNCC	1 cut(s)	240
BmrFI	CCNGG	6 cut(s)	292, 324, 912, 966, 1028, 1173
BmrI	ACTGGG	2 cut(s)	997, 1377
BmsI	GCATC	5 cut(s)	140, 652, 674, 709, 1362
BmuI	ACTGGG	2 cut(s)	997, 1377
BoxI	GACNNNNGTC	1 cut(s)	1016
BpiI	GAAGAC	1 cut(s)	409
Bpu10I	CCTNAGC	1 cut(s)	508
BpuEI	CTTGAG	2 cut(s)	182, 281
BpuMI	CCSGG	1 cut(s)	912
BsaBI	GATNNNNATC	1 cut(s)	600
BsaHI	GRCGYC	1 cut(s)	119
BsaJI	CCNNGG	7 cut(s)	67, 322, 323, 478, 965, 1152, 1172
BsaWI	WCCGGW	1 cut(s)	1444
BsaXI	ACNNNNNCTCC	6 cut(s)	345, 375, 883, 913, 1431, 1461
Bsc4I	CCNNNNNNNGG	5 cut(s)	512, 513, 692, 809, 1158
Bse1I	ACTGG	9 cut(s)	220, 375, 577, 697, 898, 992, 1042, 1080, 1372
Bse3DI	GCAATG	3 cut(s)	27, 640, 1110
Bse8I	GATNNNNATC	1 cut(s)	600
BseBI	CCWGG	5 cut(s)	292, 324, 966, 1028, 1173
BseDI	CCNNGG	7 cut(s)	67, 322, 323, 478, 965, 1152, 1172
BseGI	GGATG	2 cut(s)	64, 931
BseJI	GATNNNNATC	1 cut(s)	600
BseLI	CCNNNNNNNGG	5 cut(s)	512, 513, 692, 809, 1158
BseMI	GCAATG	3 cut(s)	27, 640, 1110
BseMII	CTCAG	5 cut(s)	357, 522, 737, 780, 969
BseNI	ACTGG	9 cut(s)	220, 375, 577, 697, 898, 992, 1042, 1080, 1372
BseXI	GCAGC	5 cut(s)	30, 148, 157, 540, 817
BsgI	GTGCAG	1 cut(s)	164
Bsh1236I	CGCG	1 cut(s)	746
BshFI	GGCC	3 cut(s)	203, 755, 802
BsiSI	CCGG	3 cut(s)	19, 911, 1445
BslFI	GGGAC	2 cut(s)	340, 475
BslI	CCNNNNNNNGG	5 cut(s)	512, 513, 692, 809, 1158
BsmAI	GTCTC	3 cut(s)	785, 818, 1061
BsmBI	CGTCTC	1 cut(s)	785
BsmFI	GGGAC	2 cut(s)	340, 475
BsmI	GAATGC	2 cut(s)	1153, 1253
BsnI	GGCC	3 cut(s)	203, 755, 802
Bsp1407I	TGTACA	1 cut(s)	930
Bsp143I	GATC	6 cut(s)	109, 333, 453, 494, 682, 812
BspACI	CCGC	2 cut(s)	551, 1437
BspANI	GGCC	3 cut(s)	203, 755, 802
BspCNI	CTCAG	5 cut(s)	358, 521, 736, 781, 970
BspFNI	CGCG	1 cut(s)	746
BspLI	GGNNCC	1 cut(s)	240
BspPI	GGATC	2 cut(s)	677, 820
BsrBI	CCGCTC	1 cut(s)	1437
BsrDI	GCAATG	3 cut(s)	27, 640, 1110
BsrGI	TGTACA	1 cut(s)	930
BsrI	ACTGG	9 cut(s)	220, 375, 577, 697, 898, 992, 1042, 1080, 1372
BssECI	CCNNGG	7 cut(s)	67, 322, 323, 478, 965, 1152, 1172
BssMI	GATC	6 cut(s)	109, 333, 453, 494, 682, 812
BssNI	GRCGYC	1 cut(s)	119
BssT1I	CCWWGG	1 cut(s)	1152
Bst2UI	CCWGG	5 cut(s)	292, 324, 966, 1028, 1173
Bst4CI	ACNGT	8 cut(s)	95, 270, 305, 349, 1187, 1234, 1262, 1384
Bst6I	CTCTTC	1 cut(s)	1429
BstACI	GRCGYC	1 cut(s)	119
BstAPI	GCANNNNNTGC	1 cut(s)	142
BstAUI	TGTACA	1 cut(s)	930
BstC8I	GCNNGC	4 cut(s)	402, 800, 1208, 1297
BstDEI	CTNAG	6 cut(s)	366, 447, 508, 723, 789, 978
BstENI	CCTNNNNNAGG	1 cut(s)	807
BstF5I	GGATG	2 cut(s)	64, 931
BstFNI	CGCG	1 cut(s)	746
BstHHI	GCGC	1 cut(s)	748
BstKTI	GATC	6 cut(s)	112, 336, 456, 497, 685, 815
BstMAI	GTCTC	3 cut(s)	785, 818, 1061
BstMBI	GATC	6 cut(s)	109, 333, 453, 494, 682, 812
BstMWI	GCNNNNNNNGC	5 cut(s)	142, 145, 752, 1207, 1250
BstNI	CCWGG	5 cut(s)	292, 324, 966, 1028, 1173
BstNSI	RCATGY	1 cut(s)	715
BstPAI	GACNNNNGTC	1 cut(s)	1016
BstSCI	CCNGG	6 cut(s)	290, 322, 910, 964, 1026, 1171
BstSFI	CTRYAG	1 cut(s)	1186
BstUI	CGCG	1 cut(s)	746
BstV1I	GCAGC	5 cut(s)	30, 148, 157, 540, 817
BstV2I	GAAGAC	1 cut(s)	409
BstXI	CCANNNNNNTGG	1 cut(s)	1277
BsuI	GTATCC	1 cut(s)	317
BsuRI	GGCC	3 cut(s)	203, 755, 802
BtgZI	GCGATG	1 cut(s)	117
BtsCI	GGATG	2 cut(s)	64, 931
BtsIMutI	CAGTG	6 cut(s)	45, 213, 690, 786, 891, 1035
Cac8I	GCNNGC	4 cut(s)	402, 800, 1208, 1297
CfoI	GCGC	1 cut(s)	748
Cfr13I	GGNCC	2 cut(s)	754, 1169
CseI	GACGC	5 cut(s)	127, 525, 740, 1006, 1233
Csp6I	GTAC	2 cut(s)	75, 931
CviAII	CATG	8 cut(s)	406, 533, 589, 712, 878, 1104, 1241, 1480
CviQI	GTAC	2 cut(s)	75, 931
DdeI	CTNAG	6 cut(s)	366, 447, 508, 723, 789, 978
DpnI	GATC	6 cut(s)	111, 335, 455, 496, 684, 814
DpnII	GATC	6 cut(s)	109, 333, 453, 494, 682, 812
DrdI	GACNNNNNNGTC	1 cut(s)	1480
DseDI	GACNNNNNNGTC	1 cut(s)	1480
Eam1104I	CTCTTC	1 cut(s)	1429
EarI	CTCTTC	1 cut(s)	1429
Eco130I	CCWWGG	1 cut(s)	1152
Eco147I	AGGCCT	1 cut(s)	203
Eco47I	GGWCC	1 cut(s)	1169
Eco57I	CTGAAG	2 cut(s)	21, 378
EcoNI	CCTNNNNNAGG	1 cut(s)	807
EcoRI	GAATTC	1 cut(s)	560
EcoRII	CCWGG	5 cut(s)	290, 322, 964, 1026, 1171
EcoT14I	CCWWGG	1 cut(s)	1152
EcoT22I	ATGCAT	1 cut(s)	667
ErhI	CCWWGG	1 cut(s)	1152
Esp3I	CGTCTC	1 cut(s)	785
FaeI	CATG	8 cut(s)	409, 536, 592, 715, 881, 1107, 1244, 1483
FaqI	GGGAC	2 cut(s)	340, 475
FatI	CATG	8 cut(s)	405, 532, 588, 711, 877, 1103, 1240, 1479
FblI	GTMKAC	2 cut(s)	285, 1020
Fnu4HI	GCNGC	5 cut(s)	44, 137, 146, 529, 831
FokI	GGATG	2 cut(s)	71, 938
Fsp4HI	GCNGC	5 cut(s)	44, 137, 146, 529, 831
FspBI	CTAG	2 cut(s)	1014, 1203
GlaI	GCGC	1 cut(s)	747
GluI	GCNGC	5 cut(s)	44, 137, 146, 529, 831
HaeIII	GGCC	3 cut(s)	203, 755, 802
HapII	CCGG	3 cut(s)	19, 911, 1445
HgaI	GACGC	5 cut(s)	127, 525, 740, 1006, 1233
HhaI	GCGC	1 cut(s)	748
Hin1I	GRCGYC	1 cut(s)	119
Hin1II	CATG	8 cut(s)	409, 536, 592, 715, 881, 1107, 1244, 1483
Hin6I	GCGC	1 cut(s)	746
HinP1I	GCGC	1 cut(s)	746
HinfI	GANTC	4 cut(s)	179, 623, 871, 974
HpaII	CCGG	3 cut(s)	19, 911, 1445
HphI	GGTGA	2 cut(s)	573, 1483
Hpy166II	GTNNAC	4 cut(s)	286, 931, 1021, 1183
Hpy188I	TCNGA	7 cut(s)	117, 250, 673, 726, 979, 1132, 1307
Hpy188III	TCNNGA	4 cut(s)	298, 492, 544, 839
Hpy8I	GTNNAC	4 cut(s)	286, 931, 1021, 1183
Hpy99I	CGWCG	1 cut(s)	121
HpyAV	CCTTC	2 cut(s)	595, 813
HpyCH4III	ACNGT	8 cut(s)	95, 270, 305, 349, 1187, 1234, 1262, 1384
HpyF10VI	GCNNNNNNNGC	5 cut(s)	142, 145, 752, 1207, 1250
HpyF3I	CTNAG	6 cut(s)	366, 447, 508, 723, 789, 978
Hsp92I	GRCGYC	1 cut(s)	119
Hsp92II	CATG	8 cut(s)	409, 536, 592, 715, 881, 1107, 1244, 1483
HspAI	GCGC	1 cut(s)	746
Kzo9I	GATC	6 cut(s)	109, 333, 453, 494, 682, 812
LmnI	GCTCC	3 cut(s)	153, 353, 1371
Lsp1109I	GCAGC	5 cut(s)	30, 148, 157, 540, 817
LweI	GCATC	5 cut(s)	140, 652, 674, 709, 1362
MaeI	CTAG	2 cut(s)	1014, 1203
MaeIII	GTNAC	6 cut(s)	361, 579, 637, 784, 1075, 1471
MalI	GATC	6 cut(s)	111, 335, 455, 496, 684, 814
MbiI	CCGCTC	1 cut(s)	1437
MboI	GATC	6 cut(s)	109, 333, 453, 494, 682, 812
MboII	GAAGA	9 cut(s)	7, 104, 343, 409, 463, 928, 1401, 1410, 1416
MluCI	AATT	7 cut(s)	84, 244, 379, 458, 560, 760, 865
MlyI	GAGTC	1 cut(s)	173
MmeI	TCCRAC	5 cut(s)	140, 207, 331, 537, 951
MnlI	CCTC	9 cut(s)	62, 488, 513, 517, 575, 912, 975, 1301, 1432
Mph1103I	ATGCAT	1 cut(s)	667
MroXI	GAANNNNTTC	1 cut(s)	1045
MseI	TTAA	4 cut(s)	159, 428, 522, 885
MslI	CAYNNNNRTG	4 cut(s)	38, 710, 1102, 1358
MspA1I	CMGCKG	2 cut(s)	553, 830
MspI	CCGG	3 cut(s)	19, 911, 1445
MspR9I	CCNGG	6 cut(s)	292, 324, 912, 966, 1028, 1173
Mva1269I	GAATGC	2 cut(s)	1153, 1253
MvaI	CCWGG	5 cut(s)	292, 324, 966, 1028, 1173
MvnI	CGCG	1 cut(s)	746
MwoI	GCNNNNNNNGC	5 cut(s)	142, 145, 752, 1207, 1250
NciI	CCSGG	1 cut(s)	912
NdeII	GATC	6 cut(s)	109, 333, 453, 494, 682, 812
NlaIII	CATG	8 cut(s)	409, 536, 592, 715, 881, 1107, 1244, 1483
NlaIV	GGNNCC	1 cut(s)	240
NmeAIII	GCCGAG	1 cut(s)	92
NmuCI	GTSAC	4 cut(s)	579, 637, 784, 1471
NsiI	ATGCAT	1 cut(s)	667
NspI	RCATGY	1 cut(s)	715
OliI	CACNNNNGTG	1 cut(s)	38
PasI	CCCWGGG	1 cut(s)	323
PceI	AGGCCT	1 cut(s)	203
PctI	GAATGC	2 cut(s)	1153, 1253
PdmI	GAANNNNTTC	1 cut(s)	1045
PfeI	GAWTC	3 cut(s)	623, 871, 974
PflMI	CCANNNNNTGG	1 cut(s)	692
PkrI	GCNGC	5 cut(s)	45, 138, 147, 530, 832
PleI	GAGTC	1 cut(s)	173
PpsI	GAGTC	1 cut(s)	173
PshAI	GACNNNNGTC	1 cut(s)	1016
Psp6I	CCWGG	5 cut(s)	290, 322, 964, 1026, 1171
PspGI	CCWGG	5 cut(s)	290, 322, 964, 1026, 1171
PspN4I	GGNNCC	1 cut(s)	240
PspPI	GGNCC	2 cut(s)	754, 1169
PvuII	CAGCTG	1 cut(s)	830
RsaI	GTAC	2 cut(s)	76, 932
RsaNI	GTAC	2 cut(s)	75, 931
RseI	CAYNNNNRTG	4 cut(s)	38, 710, 1102, 1358
SaqAI	TTAA	4 cut(s)	159, 428, 522, 885
SatI	GCNGC	5 cut(s)	44, 137, 146, 529, 831
Sau3AI	GATC	6 cut(s)	109, 333, 453, 494, 682, 812
Sau96I	GGNCC	2 cut(s)	754, 1169
SchI	GAGTC	1 cut(s)	173
ScrFI	CCNGG	6 cut(s)	292, 324, 912, 966, 1028, 1173
SetI	ASST	9 cut(s)	141, 150, 293, 358, 832, 1029, 1195, 1326, 1343
SfaNI	GCATC	5 cut(s)	140, 652, 674, 709, 1362
SfcI	CTRYAG	1 cut(s)	1186
SinI	GGWCC	1 cut(s)	1169
SmiMI	CAYNNNNRTG	4 cut(s)	38, 710, 1102, 1358
SmlI	CTYRAG	2 cut(s)	197, 296
SmoI	CTYRAG	2 cut(s)	197, 296
Sse9I	AATT	7 cut(s)	84, 244, 379, 458, 560, 760, 865
SseBI	AGGCCT	1 cut(s)	203
SsiI	CCGC	2 cut(s)	551, 1437
SspMI	CTAG	2 cut(s)	1014, 1203
StuI	AGGCCT	1 cut(s)	203
StyD4I	CCNGG	6 cut(s)	290, 322, 910, 964, 1026, 1171
StyI	CCWWGG	1 cut(s)	1152
TaaI	ACNGT	8 cut(s)	95, 270, 305, 349, 1187, 1234, 1262, 1384
TaqI	TCGA	5 cut(s)	717, 1118, 1137, 1218, 1326
TasI	AATT	7 cut(s)	84, 244, 379, 458, 560, 760, 865
TatI	WGTACW	1 cut(s)	930
TfiI	GAWTC	3 cut(s)	623, 871, 974
Tru1I	TTAA	4 cut(s)	159, 428, 522, 885
Tru9I	TTAA	4 cut(s)	159, 428, 522, 885
TscAI	CASTG	6 cut(s)	45, 220, 697, 793, 898, 1042
TseFI	GTSAC	4 cut(s)	579, 637, 784, 1471
TseI	GCWGC	5 cut(s)	43, 136, 145, 528, 830
Tsp45I	GTSAC	4 cut(s)	579, 637, 784, 1471
TspDTI	ATGAA	2 cut(s)	577, 1468
TspRI	CASTG	6 cut(s)	45, 220, 697, 793, 898, 1042
Van91I	CCANNNNNTGG	1 cut(s)	692
VpaK11BI	GGWCC	1 cut(s)	1169
XagI	CCTNNNNNAGG	1 cut(s)	807
XapI	RAATTY	4 cut(s)	84, 244, 560, 760
XceI	RCATGY	1 cut(s)	715
XmiI	GTMKAC	2 cut(s)	285, 1020
XmnI	GAANNNNTTC	1 cut(s)	1045
XspI	CTAG	2 cut(s)	1014, 1203
Zsp2I	ATGCAT	1 cut(s)	667

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.