Rorug02G0023600

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006108
Physical Location & Seq
Forward (+)
1871820 .. 1874381
2562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0023600.1

Sequence Viewer

Length: 1227 bp
ATGAGTCCCGGGGAAGGAGGAGGAGGAGCTGCTGTAAATTTCAGGAAGAGATCAAAACGACGGTCAAACAATGCCGCCGGAGCTACGAAAGATCAACCCCAAAACCATGAAGATAGTTGTTATTACCAGTCATCTTCTTCGTCGTCCTCTTCGGCTTCTAATTATGAGAGCAAGAAGAAGAGGATTAGGAAGAAGAAGGGTAAAGGGAACAACAACAACGTTAGGGGGTCGTCGAAACGGTTCCAGCTGATGTCGTATTGGGAGTTGCCGGAATATATGAAGGATAACGAGTTCATTTTGAATTATTACCGAGCTGATTGGCCTATTCTCGAAGCTCTTTTCAGCGTTTTCCGTTGGCATAATGAAACTCTCAACGTTTGGACGCATTTGATTGGGTTTGTTCTGTTTGTGGGTTTGACTATGGCGAATTTGTGGGAAGTGCCTCAGGTAGCGGATCTCCTTGGCTTTTTTAACAGGTCCACATTTAGTGCGGCTGCAAATGTTTCCCATAATTTTAGTGCGCAGGAGACAACAAACTTTGTTCATGATTTGAAGCAAATAACATCTCAGGAAATGAACAACATTGCATCGCCTGGTGATCGATTGAGCGTGGCACTTTGGCCGTTTTATGTGTTCCTAGCTGGCTCTATGTTCTGCCTTTTCTCCAGCAGCATTTGCCATCTCTTTTCATGCCACTCTCACCCCTTAAACGTCTTGCTCTTGCGCATAGATTACGCAGGCATCACCACCATGATCATCACCTCATTTTTCCCTCCGATTTACTACATCTTCCAATGTGATCCGCGCTGGCAATTCATTTACCTCGGCGGCATCACAATAATGGGAATTTGCACCATCATCACACTGTTCTCCCCGAAGCTTTCTAGCTCCAAATTTCGAACATTTCGAGCTTTGCTTTTTGCTTCACTGGGGTTGTTTGGGATTGTGCCTGCAGTCCATGCCAGCGTTGTGAATTGGGGCAATCCGAAGCGTGATATCACTCTGGTTTATGAGGTTGCCATGGCGCTTTTCTATTTGACAGGGACCGGATTTTACATCACTCGAATTCCCGAGAGATGGAGACCGGGGTGCTTCGATTTGGCAGGTCACAGCCATCAGATTTTTCATGTTTTGGTGGTGATGGGTGCATTGGCTCATTATGCTGCCATACTTGTAATGCTAGACTGGCGAAGCACCTTGGGGTGTGACATCAATATTACTCTGTAA

Protein Analysis

408

Amino Acids

46.12

Weight (kDa)

9.25

Isoelectric Point (pI)

57.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HlyIII PF03006 117 - 387 7e-61 Haemolysin-III related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 522, 725
Acc36I ACCTGC 1 cut(s) 1094
AccII CGCG 1 cut(s) 805
AciI CCGC 5 cut(s) 75, 452, 491, 803, 828
AclI AACGTT 2 cut(s) 219, 375
AclWI GGATC 2 cut(s) 462, 794
AcoI YGGCCR 1 cut(s) 620
AcsI RAATTY 5 cut(s) 37, 427, 846, 893, 1065
AfiI CCNNNNNNNGG 2 cut(s) 14, 1077
AgsI TTSAA 2 cut(s) 301, 553
AjnI CCWGG 1 cut(s) 592
AloI GAACNNNNNNTCC 2 cut(s) 275, 307
AluBI AGCT 9 cut(s) 29, 83, 247, 314, 335, 641, 880, 888, 911
AluI AGCT 9 cut(s) 29, 83, 247, 314, 335, 641, 880, 888, 911
Alw26I GTCTC 2 cut(s) 521, 1075
AlwI GGATC 2 cut(s) 462, 794
Ama87I CYCGRG 2 cut(s) 8, 1070
AoxI GGCC 2 cut(s) 320, 620
ApeKI GCWGC 4 cut(s) 29, 494, 669, 1163
ApoI RAATTY 5 cut(s) 37, 427, 846, 893, 1065
ArsI GACNNNNNNTTYG 2 cut(s) 47, 79
Asp700I GAANNNNTTC 1 cut(s) 239
AspLEI GCGC 4 cut(s) 523, 726, 807, 1027
AspS9I GGNCC 2 cut(s) 477, 1044
AsuC2I CCSGG 3 cut(s) 9, 10, 1086
AsuHPI GGTGA 5 cut(s) 608, 692, 736, 751, 1150
AsuII TTCGAA 1 cut(s) 898
AvaI CYCGRG 2 cut(s) 8, 1070
AvaII GGWCC 2 cut(s) 477, 1044
AxyI CCTNAGG 1 cut(s) 444
BbvI GCAGC 4 cut(s) 16, 481, 681, 1150
BccI CCATC 5 cut(s) 687, 863, 1071, 1122, 1135
BceAI ACGGC 1 cut(s) 607
BciT130I CCWGG 1 cut(s) 594
BclI TGATCA 1 cut(s) 753
BcnI CCSGG 3 cut(s) 9, 10, 1086
BcoDI GTCTC 2 cut(s) 521, 1075
BfaI CTAG 3 cut(s) 638, 885, 1181
BfmI CTRYAG 1 cut(s) 951
BfoI RGCGCY 1 cut(s) 1028
BfuAI ACCTGC 1 cut(s) 1094
BisI GCNGC 7 cut(s) 30, 75, 492, 495, 670, 829, 1164
BlsI GCNGC 7 cut(s) 31, 76, 493, 496, 671, 830, 1165
Bme1390I CCNGG 4 cut(s) 9, 10, 594, 1086
Bme18I GGWCC 2 cut(s) 477, 1044
BmeT110I CYCGRG 2 cut(s) 8, 1070
BmgT120I GGNCC 2 cut(s) 477, 1044
BmiI GGNNCC 2 cut(s) 242, 1045
BmrFI CCNGG 4 cut(s) 9, 10, 594, 1086
BmrI ACTGGG 1 cut(s) 938
BmsI GCATC 3 cut(s) 596, 750, 840
BmuI ACTGGG 1 cut(s) 938
BpmI CTGGAG 1 cut(s) 649
Bpu14I TTCGAA 1 cut(s) 898
BpuMI CCSGG 3 cut(s) 9, 10, 1086
Bsa29I ATCGAT 1 cut(s) 601
BsaI GGTCTC 1 cut(s) 1075
BsaJI CCNNGG 7 cut(s) 8, 9, 460, 823, 1020, 1085, 1197
BsaWI WCCGGW 1 cut(s) 1046
BsaXI ACNNNNNCTCC 2 cut(s) 18, 48
Bsc4I CCNNNNNNNGG 2 cut(s) 14, 1077
Bse1I ACTGG 3 cut(s) 127, 933, 1190
Bse21I CCTNAGG 1 cut(s) 444
Bse3DI GCAATG 1 cut(s) 582
BseBI CCWGG 1 cut(s) 594
BseCI ATCGAT 1 cut(s) 601
BseDI CCNNGG 7 cut(s) 8, 9, 460, 823, 1020, 1085, 1197
BseLI CCNNNNNNNGG 2 cut(s) 14, 1077
BseMI GCAATG 1 cut(s) 582
BseMII CTCAG 2 cut(s) 458, 581
BseNI ACTGG 3 cut(s) 127, 933, 1190
BseRI GAGGAG 3 cut(s) 33, 36, 39
BseXI GCAGC 4 cut(s) 16, 481, 681, 1150
Bsh1236I CGCG 1 cut(s) 805
BshFI GGCC 2 cut(s) 322, 622
BshVI ATCGAT 1 cut(s) 601
BsiHKCI CYCGRG 2 cut(s) 8, 1070
BsiSI CCGG 5 cut(s) 9, 78, 269, 1047, 1085
BslFI GGGAC 1 cut(s) 1057
BslI CCNNNNNNNGG 2 cut(s) 14, 1077
BsmAI GTCTC 2 cut(s) 521, 1075
BsmFI GGGAC 1 cut(s) 1057
BsnI GGCC 2 cut(s) 322, 622
Bso31I GGTCTC 1 cut(s) 1075
BsoBI CYCGRG 2 cut(s) 8, 1070
Bsp119I TTCGAA 1 cut(s) 898
Bsp143I GATC 6 cut(s) 50, 91, 454, 598, 753, 799
Bsp19I CCATGG 1 cut(s) 1020
BspACI CCGC 5 cut(s) 75, 452, 491, 803, 828
BspANI GGCC 2 cut(s) 322, 622
BspCNI CTCAG 2 cut(s) 457, 580
BspDI ATCGAT 1 cut(s) 601
BspFNI CGCG 1 cut(s) 805
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 2 cut(s) 242, 1045
BspMAI CTGCAG 1 cut(s) 955
BspMI ACCTGC 1 cut(s) 1094
BspPI GGATC 2 cut(s) 462, 794
BspT104I TTCGAA 1 cut(s) 898
BspTNI GGTCTC 1 cut(s) 1075
BsrDI GCAATG 1 cut(s) 582
BsrI ACTGG 3 cut(s) 127, 933, 1190
BssECI CCNNGG 7 cut(s) 8, 9, 460, 823, 1020, 1085, 1197
BssMI GATC 6 cut(s) 50, 91, 454, 598, 753, 799
BssT1I CCWWGG 3 cut(s) 460, 1020, 1197
Bst2UI CCWGG 1 cut(s) 594
Bst4CI ACNGT 3 cut(s) 63, 240, 867
Bst6I CTCTTC 3 cut(s) 41, 154, 173
BstAPI GCANNNNNTGC 2 cut(s) 675, 959
BstBI TTCGAA 1 cut(s) 898
BstC8I GCNNGC 5 cut(s) 643, 739, 809, 951, 964
BstDEI CTNAG 2 cut(s) 444, 567
BstDSI CCRYGG 1 cut(s) 1020
BstFNI CGCG 1 cut(s) 805
BstH2I RGCGCY 1 cut(s) 1028
BstHHI GCGC 4 cut(s) 523, 726, 807, 1027
BstKTI GATC 6 cut(s) 53, 94, 457, 601, 756, 802
BstMAI GTCTC 2 cut(s) 521, 1075
BstMBI GATC 6 cut(s) 50, 91, 454, 598, 753, 799
BstMWI GCNNNNNNNGC 5 cut(s) 80, 675, 959, 1160, 1186
BstNI CCWGG 1 cut(s) 594
BstSCI CCNGG 4 cut(s) 7, 8, 592, 1084
BstSFI CTRYAG 1 cut(s) 951
BstUI CGCG 1 cut(s) 805
BstV1I GCAGC 4 cut(s) 16, 481, 681, 1150
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
Bsu15I ATCGAT 1 cut(s) 601
Bsu36I CCTNAGG 1 cut(s) 444
BsuRI GGCC 2 cut(s) 322, 622
BsuTUI ATCGAT 1 cut(s) 601
BtgI CCRYGG 1 cut(s) 1020
BtgZI GCGATG 1 cut(s) 573
BtsIMutI CAGTG 2 cut(s) 863, 926
BveI ACCTGC 1 cut(s) 1094
Cac8I GCNNGC 5 cut(s) 643, 739, 809, 951, 964
CciI TCATGA 1 cut(s) 544
CfoI GCGC 4 cut(s) 523, 726, 807, 1027
Cfr13I GGNCC 2 cut(s) 477, 1044
Cfr9I CCCGGG 1 cut(s) 8
ClaI ATCGAT 1 cut(s) 601
CseI GACGC 1 cut(s) 391
CviAII CATG 7 cut(s) 107, 545, 690, 751, 959, 1021, 1127
DdeI CTNAG 2 cut(s) 444, 567
DpnI GATC 6 cut(s) 52, 93, 456, 600, 755, 801
DpnII GATC 6 cut(s) 50, 91, 454, 598, 753, 799
EaeI YGGCCR 1 cut(s) 620
Eam1104I CTCTTC 3 cut(s) 41, 154, 173
EarI CTCTTC 3 cut(s) 41, 154, 173
Eco130I CCWWGG 3 cut(s) 460, 1020, 1197
Eco31I GGTCTC 1 cut(s) 1075
Eco32I GATATC 1 cut(s) 997
Eco47I GGWCC 2 cut(s) 477, 1044
Eco81I CCTNAGG 1 cut(s) 444
Eco88I CYCGRG 2 cut(s) 8, 1070
EcoRI GAATTC 1 cut(s) 1065
EcoRII CCWGG 1 cut(s) 592
EcoRV GATATC 1 cut(s) 997
EcoT14I CCWWGG 3 cut(s) 460, 1020, 1197
ErhI CCWWGG 3 cut(s) 460, 1020, 1197
FaeI CATG 7 cut(s) 110, 548, 693, 754, 962, 1024, 1130
FaqI GGGAC 1 cut(s) 1057
FatI CATG 7 cut(s) 106, 544, 689, 750, 958, 1020, 1126
FbaI TGATCA 1 cut(s) 753
Fnu4HI GCNGC 7 cut(s) 30, 75, 492, 495, 670, 829, 1164
Fsp4HI GCNGC 7 cut(s) 30, 75, 492, 495, 670, 829, 1164
FspBI CTAG 3 cut(s) 638, 885, 1181
FspI TGCGCA 2 cut(s) 522, 725
GlaI GCGC 4 cut(s) 522, 725, 806, 1026
GluI GCNGC 7 cut(s) 30, 75, 492, 495, 670, 829, 1164
GsuI CTGGAG 1 cut(s) 649
HaeII RGCGCY 1 cut(s) 1028
HaeIII GGCC 2 cut(s) 322, 622
HapII CCGG 5 cut(s) 9, 78, 269, 1047, 1085
HgaI GACGC 1 cut(s) 391
HhaI GCGC 4 cut(s) 523, 726, 807, 1027
Hin1II CATG 7 cut(s) 110, 548, 693, 754, 962, 1024, 1130
Hin6I GCGC 4 cut(s) 521, 724, 805, 1025
HinP1I GCGC 4 cut(s) 521, 724, 805, 1025
HindIII AAGCTT 1 cut(s) 878
HinfI GANTC 1 cut(s) 4
HpaII CCGG 5 cut(s) 9, 78, 269, 1047, 1085
HphI GGTGA 5 cut(s) 608, 692, 736, 751, 1150
Hpy166II GTNNAC 1 cut(s) 480
Hpy188I TCNGA 3 cut(s) 777, 987, 1119
Hpy188III TCNNGA 5 cut(s) 43, 329, 545, 569, 1070
Hpy8I GTNNAC 1 cut(s) 480
Hpy99I CGWCG 3 cut(s) 63, 145, 235
HpyAV CCTTC 3 cut(s) 8, 190, 274
HpyCH4III ACNGT 3 cut(s) 63, 240, 867
HpyCH4IV ACGT 3 cut(s) 219, 375, 711
HpyCH4V TGCA 5 cut(s) 497, 587, 852, 953, 1148
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 675, 959, 1160, 1186
HpyF3I CTNAG 2 cut(s) 444, 567
HpySE526I ACGT 3 cut(s) 219, 375, 711
Hsp92II CATG 7 cut(s) 110, 548, 693, 754, 962, 1024, 1130
HspAI GCGC 4 cut(s) 521, 724, 805, 1025
Ksp22I TGATCA 1 cut(s) 753
Kzo9I GATC 6 cut(s) 50, 91, 454, 598, 753, 799
LmnI GCTCC 3 cut(s) 26, 80, 893
Lsp1109I GCAGC 4 cut(s) 16, 481, 681, 1150
LweI GCATC 3 cut(s) 596, 750, 840
MaeI CTAG 3 cut(s) 638, 885, 1181
MaeII ACGT 3 cut(s) 219, 375, 711
MaeIII GTNAC 2 cut(s) 1106, 1205
MalI GATC 6 cut(s) 52, 93, 456, 600, 755, 801
MboI GATC 6 cut(s) 50, 91, 454, 598, 753, 799
MflI RGATCY 1 cut(s) 454
MlyI GAGTC 1 cut(s) 13
MroXI GAANNNNTTC 1 cut(s) 239
MseI TTAA 2 cut(s) 471, 707
MslI CAYNNNNRTG 2 cut(s) 749, 839
MspA1I CMGCKG 1 cut(s) 247
MspI CCGG 5 cut(s) 9, 78, 269, 1047, 1085
MspR9I CCNGG 4 cut(s) 9, 10, 594, 1086
MvaI CCWGG 1 cut(s) 594
MvnI CGCG 1 cut(s) 805
MwoI GCNNNNNNNGC 5 cut(s) 80, 675, 959, 1160, 1186
NciI CCSGG 3 cut(s) 9, 10, 1086
NcoI CCATGG 1 cut(s) 1020
NdeII GATC 6 cut(s) 50, 91, 454, 598, 753, 799
NlaIII CATG 7 cut(s) 110, 548, 693, 754, 962, 1024, 1130
NlaIV GGNNCC 2 cut(s) 242, 1045
NmeAIII GCCGAG 1 cut(s) 804
NmuCI GTSAC 2 cut(s) 1106, 1205
NsbI TGCGCA 2 cut(s) 522, 725
NspV TTCGAA 1 cut(s) 898
PagI TCATGA 1 cut(s) 544
PcsI WCGNNNNNNNCGW 1 cut(s) 904
PdmI GAANNNNTTC 1 cut(s) 239
PkrI GCNGC 7 cut(s) 31, 76, 493, 496, 671, 830, 1165
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
Psp1406I AACGTT 2 cut(s) 219, 375
Psp6I CCWGG 1 cut(s) 592
PspGI CCWGG 1 cut(s) 592
PspN4I GGNNCC 2 cut(s) 242, 1045
PspPI GGNCC 2 cut(s) 477, 1044
PstI CTGCAG 1 cut(s) 955
PsuI RGATCY 1 cut(s) 454
PvuII CAGCTG 1 cut(s) 247
RseI CAYNNNNRTG 2 cut(s) 749, 839
SaqAI TTAA 2 cut(s) 471, 707
SatI GCNGC 7 cut(s) 30, 75, 492, 495, 670, 829, 1164
Sau3AI GATC 6 cut(s) 50, 91, 454, 598, 753, 799
Sau96I GGNCC 2 cut(s) 477, 1044
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 4 cut(s) 9, 10, 594, 1086
SfaNI GCATC 3 cut(s) 596, 750, 840
SfcI CTRYAG 1 cut(s) 951
SfuI TTCGAA 1 cut(s) 898
SinI GGWCC 2 cut(s) 477, 1044
SmaI CCCGGG 1 cut(s) 10
SmiMI CAYNNNNRTG 2 cut(s) 749, 839
SsiI CCGC 5 cut(s) 75, 452, 491, 803, 828
SspI AATATT 1 cut(s) 1216
SspMI CTAG 3 cut(s) 638, 885, 1181
StyD4I CCNGG 4 cut(s) 7, 8, 592, 1084
StyI CCWWGG 3 cut(s) 460, 1020, 1197
TaaI ACNGT 3 cut(s) 63, 240, 867
TaiI ACGT 3 cut(s) 222, 378, 714
TaqI TCGA 7 cut(s) 233, 330, 601, 898, 907, 1063, 1095
TauI GCSGC 3 cut(s) 77, 494, 831
Tru1I TTAA 2 cut(s) 471, 707
Tru9I TTAA 2 cut(s) 471, 707
TscAI CASTG 2 cut(s) 870, 933
TseFI GTSAC 2 cut(s) 1106, 1205
TseI GCWGC 4 cut(s) 29, 494, 669, 1163
Tsp45I GTSAC 2 cut(s) 1106, 1205
TspDTI ATGAA 9 cut(s) 123, 283, 293, 378, 533, 590, 678, 805, 1115
TspGWI ACGGA 1 cut(s) 341
TspMI CCCGGG 1 cut(s) 8
TspRI CASTG 2 cut(s) 870, 933
VpaK11BI GGWCC 2 cut(s) 477, 1044
XapI RAATTY 5 cut(s) 37, 427, 846, 893, 1065
XmaI CCCGGG 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 239
XspI CTAG 3 cut(s) 638, 885, 1181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.