pycom07g17880

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006108
Physical Location & Seq
Forward (+)
20429020 .. 20431895
2876 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g17880.1

Sequence Viewer

Length: 960 bp
ATGGCCTCTGACATATTGGATGGTGTCAAATATGAAGAGGAGTACATCTTAAATTCTAGAGGCATGAAGCTTTTCACATGCAGATGGCTCCCGAATAACACCAAACCCAAAGCATTAATTTTCATCTGCCATGGATACGGCATGGAATGCAGCATCACCATGAATAGCACCGCAATCAGGCTAGCCAAAGCAGGCTTTGCCATATATGGAATAGACTATGACGGTCATGGAAAATCAGCAGGCCTACAAGGTTACGTGAAGAGTTTTGATGATGTGGTGGATGACTGTACCAACCACTTCACAAACATATGTGAGAGCAAAGAGAACAAGGGAAAGATGAGGTATCTAATGGGAGAGTCAATGGGAGGAACTGTGGCTCTGCTGGTGCACAGGAAAAAGCCCGAGTTTTGGGATGGTGCGGTTTTGGTTGCGCCCATGTGTAAGATTGCAGATGAGCTGAGGCCATCCCCATTAGTGATCACTGCTTTGACAAAGCTCTGCAAGTTCATTCCGACCTGGAAAATAATCCCAACAAATGATATTATTGATGTCGCTTTCAAAATGCCCGAAATAAGAAAACAGGTTAGAGAAAACCCGTACTGCTATAAAGGCCGGCCTCGTCTCCAAACCGGCTACGAACTTTTGAGGGGCAGTTCGGATCTTGAACAGAGACTCGACGAGGTCACGTTGCCGTTCATAGTTCTCCATGGCGAAGACGATAAGGTTACCGATAAATCCGTCAGCAAACAGCTCCATGAGGTAGCCTCCAGCCATGACAAGACGTTGAAGATGTACCCGGATATGTGGCATGGACTGCTCTATGGAGAAACACCTGAAAACATTGAGGTTGTTTTTTCTGATATAATCAGTTGGTTGGATGAGAGGAGTTATTTTGGAAATGCAAGGCTGGAAGGAGAGTTAAAACGTGAAAACGACGATTTACCCCAAGTAAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

320

Amino Acids

36.29

Weight (kDa)

6.11

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 35 - 273 1.2e-65 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 39 - 149 4.3e-07 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 41 - 279 1.9e-07 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 171, 419
AclWI GGATC 1 cut(s) 666
AcsI RAATTY 1 cut(s) 52
AfaI GTAC 4 cut(s) 44, 289, 599, 794
AfiI CCNNNNNNNGG 2 cut(s) 177, 408
AgsI TTSAA 3 cut(s) 559, 665, 787
AjnI CCWGG 1 cut(s) 515
AluBI AGCT 4 cut(s) 70, 457, 496, 751
AluI AGCT 4 cut(s) 70, 457, 496, 751
Alw21I GWGCWC 1 cut(s) 390
Alw26I GTCTC 2 cut(s) 626, 664
Alw44I GTGCAC 1 cut(s) 386
AlwI GGATC 1 cut(s) 666
Ama87I CYCGRG 1 cut(s) 401
AoxI GGCC 5 cut(s) 3, 241, 461, 610, 614
ApaLI GTGCAC 1 cut(s) 386
ApeKI GCWGC 1 cut(s) 150
ApoI RAATTY 1 cut(s) 52
AseI ATTAAT 1 cut(s) 116
Asp700I GAANNNNTTC 1 cut(s) 71
AspLEI GCGC 1 cut(s) 433
AsuC2I CCSGG 1 cut(s) 797
AsuHPI GGTGA 1 cut(s) 148
AsuNHI GCTAGC 1 cut(s) 181
AvaI CYCGRG 1 cut(s) 401
BaeGI GKGCMC 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 720
Bbv12I GWGCWC 1 cut(s) 390
BbvCI CCTCAGC 1 cut(s) 458
BbvI GCAGC 1 cut(s) 162
BccI CCATC 4 cut(s) 14, 78, 407, 472
BceAI ACGGC 2 cut(s) 154, 676
BciT130I CCWGG 1 cut(s) 517
BciVI GTATCC 1 cut(s) 128
BclI TGATCA 1 cut(s) 477
BcnI CCSGG 1 cut(s) 797
BcoDI GTCTC 2 cut(s) 626, 664
BfaI CTAG 2 cut(s) 57, 182
BfuI GTATCC 1 cut(s) 128
BisI GCNGC 1 cut(s) 151
BlsI GCNGC 1 cut(s) 152
Bme1390I CCNGG 2 cut(s) 517, 797
BmeT110I CYCGRG 1 cut(s) 401
BmiI GGNNCC 1 cut(s) 89
BmrFI CCNGG 2 cut(s) 517, 797
BmsI GCATC 1 cut(s) 162
BmtI GCTAGC 1 cut(s) 185
BpiI GAAGAC 1 cut(s) 720
BplI GAGNNNNNCTC 2 cut(s) 749, 781
BpmI CTGGAG 1 cut(s) 751
Bpu10I CCTNAGC 1 cut(s) 458
BpuMI CCSGG 1 cut(s) 797
BsaAI YACGTR 1 cut(s) 256
BsaJI CCNNGG 2 cut(s) 130, 706
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 408
Bse118I RCCGGY 2 cut(s) 612, 629
BseBI CCWGG 1 cut(s) 517
BseDI CCNNGG 2 cut(s) 130, 706
BseGI GGATG 5 cut(s) 25, 286, 418, 464, 883
BseLI CCNNNNNNNGG 2 cut(s) 177, 408
BseMII CTCAG 1 cut(s) 449
BseRI GAGGAG 2 cut(s) 53, 898
BseSI GKGCMC 1 cut(s) 390
BseXI GCAGC 1 cut(s) 162
BshFI GGCC 5 cut(s) 5, 243, 463, 612, 616
BsiHKAI GWGCWC 1 cut(s) 390
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 3 cut(s) 613, 630, 797
BslI CCNNNNNNNGG 2 cut(s) 177, 408
BsmAI GTCTC 2 cut(s) 626, 664
BsmBI CGTCTC 1 cut(s) 626
BsmI GAATGC 1 cut(s) 152
BsnI GGCC 5 cut(s) 5, 243, 463, 612, 616
BsoBI CYCGRG 1 cut(s) 401
Bsp1286I GDGCHC 1 cut(s) 390
Bsp143I GATC 2 cut(s) 477, 658
Bsp19I CCATGG 2 cut(s) 130, 706
BspACI CCGC 2 cut(s) 171, 419
BspANI GGCC 5 cut(s) 5, 243, 463, 612, 616
BspCNI CTCAG 1 cut(s) 450
BspLI GGNNCC 1 cut(s) 89
BspOI GCTAGC 1 cut(s) 185
BspPI GGATC 1 cut(s) 666
BsrFI RCCGGY 2 cut(s) 612, 629
BssAI RCCGGY 2 cut(s) 612, 629
BssECI CCNNGG 2 cut(s) 130, 706
BssMI GATC 2 cut(s) 477, 658
BssT1I CCWWGG 2 cut(s) 130, 706
Bst2UI CCWGG 1 cut(s) 517
Bst4CI ACNGT 3 cut(s) 224, 287, 373
Bst6I CTCTTC 2 cut(s) 30, 254
BstAPI GCANNNNNTGC 3 cut(s) 147, 197, 814
BstBAI YACGTR 1 cut(s) 256
BstC8I GCNNGC 4 cut(s) 183, 193, 241, 614
BstDEI CTNAG 1 cut(s) 458
BstDSI CCRYGG 2 cut(s) 130, 706
BstEII GGTNACC 1 cut(s) 724
BstF5I GGATG 5 cut(s) 25, 286, 418, 464, 883
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 2 cut(s) 480, 661
BstMAI GTCTC 2 cut(s) 626, 664
BstMBI GATC 2 cut(s) 477, 658
BstMWI GCNNNNNNNGC 4 cut(s) 147, 197, 609, 814
BstNI CCWGG 1 cut(s) 517
BstNSI RCATGY 1 cut(s) 81
BstPI GGTNACC 1 cut(s) 724
BstSCI CCNGG 2 cut(s) 515, 795
BstSLI GKGCMC 1 cut(s) 390
BstV1I GCAGC 1 cut(s) 162
BstV2I GAAGAC 1 cut(s) 720
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
BsuI GTATCC 1 cut(s) 128
BsuRI GGCC 5 cut(s) 5, 243, 463, 612, 616
BtgI CCRYGG 2 cut(s) 130, 706
BtsCI GGATG 5 cut(s) 25, 286, 418, 464, 883
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 1 cut(s) 480
Cac8I GCNNGC 4 cut(s) 183, 193, 241, 614
CfoI GCGC 1 cut(s) 433
Cfr10I RCCGGY 2 cut(s) 612, 629
Csp6I GTAC 4 cut(s) 43, 288, 598, 793
CviQI GTAC 4 cut(s) 43, 288, 598, 793
DdeI CTNAG 1 cut(s) 458
DpnI GATC 2 cut(s) 479, 660
DpnII GATC 2 cut(s) 477, 658
Eam1104I CTCTTC 2 cut(s) 30, 254
EarI CTCTTC 2 cut(s) 30, 254
Eco130I CCWWGG 2 cut(s) 130, 706
Eco147I AGGCCT 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 401
Eco91I GGTNACC 1 cut(s) 724
EcoO65I GGTNACC 1 cut(s) 724
EcoRII CCWGG 1 cut(s) 515
EcoT14I CCWWGG 2 cut(s) 130, 706
ErhI CCWWGG 2 cut(s) 130, 706
Esp3I CGTCTC 1 cut(s) 626
FauNDI CATATG 1 cut(s) 308
FbaI TGATCA 1 cut(s) 477
Fnu4HI GCNGC 1 cut(s) 151
FokI GGATG 5 cut(s) 32, 293, 425, 451, 890
FseI GGCCGGCC 1 cut(s) 616
Fsp4HI GCNGC 1 cut(s) 151
FspBI CTAG 2 cut(s) 57, 182
GlaI GCGC 1 cut(s) 432
GluI GCNGC 1 cut(s) 151
GsuI CTGGAG 1 cut(s) 751
HaeIII GGCC 5 cut(s) 5, 243, 463, 612, 616
HapII CCGG 3 cut(s) 613, 630, 797
HhaI GCGC 1 cut(s) 433
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HindIII AAGCTT 1 cut(s) 68
HinfI GANTC 2 cut(s) 356, 672
HpaII CCGG 3 cut(s) 613, 630, 797
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 4 cut(s) 10, 513, 658, 859
Hpy188III TCNNGA 3 cut(s) 57, 91, 662
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 2 cut(s) 680, 938
HpyAV CCTTC 1 cut(s) 905
HpyCH4III ACNGT 3 cut(s) 224, 287, 373
HpyCH4IV ACGT 4 cut(s) 255, 686, 782, 925
HpyCH4V TGCA 6 cut(s) 81, 150, 388, 449, 501, 902
HpyF10VI GCNNNNNNNGC 4 cut(s) 147, 197, 609, 814
HpyF3I CTNAG 1 cut(s) 458
HpySE526I ACGT 4 cut(s) 255, 686, 782, 925
HspAI GCGC 1 cut(s) 431
KroI GCCGGC 1 cut(s) 612
KroNI GCCGGC 1 cut(s) 614
Ksp22I TGATCA 1 cut(s) 477
Kzo9I GATC 2 cut(s) 477, 658
LmnI GCTCC 2 cut(s) 93, 756
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 1 cut(s) 162
MaeI CTAG 2 cut(s) 57, 182
MaeII ACGT 4 cut(s) 255, 686, 782, 925
MaeIII GTNAC 3 cut(s) 251, 682, 724
MalI GATC 2 cut(s) 479, 660
MboI GATC 2 cut(s) 477, 658
MboII GAAGA 4 cut(s) 47, 271, 725, 799
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 1 cut(s) 390
MluCI AATT 2 cut(s) 52, 117
MlyI GAGTC 2 cut(s) 365, 666
MmeI TCCRAC 2 cut(s) 536, 855
MroNI GCCGGC 1 cut(s) 612
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 3 cut(s) 50, 116, 920
MslI CAYNNNNRTG 2 cut(s) 82, 158
MspI CCGG 3 cut(s) 613, 630, 797
MspR9I CCNGG 2 cut(s) 517, 797
Mva1269I GAATGC 1 cut(s) 152
MvaI CCWGG 1 cut(s) 517
MwoI GCNNNNNNNGC 4 cut(s) 147, 197, 609, 814
NaeI GCCGGC 1 cut(s) 614
NciI CCSGG 1 cut(s) 797
NcoI CCATGG 2 cut(s) 130, 706
NdeI CATATG 1 cut(s) 308
NdeII GATC 2 cut(s) 477, 658
NgoMIV GCCGGC 1 cut(s) 612
NheI GCTAGC 1 cut(s) 181
NlaIV GGNNCC 1 cut(s) 89
NmuCI GTSAC 1 cut(s) 682
NspI RCATGY 1 cut(s) 81
PceI AGGCCT 1 cut(s) 243
PctI GAATGC 1 cut(s) 152
PdiI GCCGGC 1 cut(s) 614
PdmI GAANNNNTTC 1 cut(s) 71
PflFI GACNNNGTC 1 cut(s) 680
PkrI GCNGC 1 cut(s) 152
PleI GAGTC 2 cut(s) 364, 666
PpsI GAGTC 2 cut(s) 364, 666
Ppu21I YACGTR 1 cut(s) 256
PshBI ATTAAT 1 cut(s) 116
Psp6I CCWGG 1 cut(s) 515
PspEI GGTNACC 1 cut(s) 724
PspGI CCWGG 1 cut(s) 515
PspN4I GGNNCC 1 cut(s) 89
PsuI RGATCY 1 cut(s) 658
PsyI GACNNNGTC 1 cut(s) 680
RigI GGCCGGCC 1 cut(s) 616
RsaI GTAC 4 cut(s) 44, 289, 599, 794
RsaNI GTAC 4 cut(s) 43, 288, 598, 793
RseI CAYNNNNRTG 2 cut(s) 82, 158
SaqAI TTAA 3 cut(s) 50, 116, 920
SatI GCNGC 1 cut(s) 151
Sau3AI GATC 2 cut(s) 477, 658
SchI GAGTC 2 cut(s) 365, 666
ScrFI CCNGG 2 cut(s) 517, 797
SduI GDGCHC 1 cut(s) 390
SfaNI GCATC 1 cut(s) 162
SmiMI CAYNNNNRTG 2 cut(s) 82, 158
Sse9I AATT 2 cut(s) 52, 117
SseBI AGGCCT 1 cut(s) 243
SsiI CCGC 2 cut(s) 171, 419
SspMI CTAG 2 cut(s) 57, 182
StuI AGGCCT 1 cut(s) 243
StyD4I CCNGG 2 cut(s) 515, 795
StyI CCWWGG 2 cut(s) 130, 706
TaaI ACNGT 3 cut(s) 224, 287, 373
TaiI ACGT 4 cut(s) 258, 689, 785, 928
TaqI TCGA 1 cut(s) 675
TasI AATT 2 cut(s) 52, 117
TatI WGTACW 1 cut(s) 42
Tru1I TTAA 3 cut(s) 50, 116, 920
Tru9I TTAA 3 cut(s) 50, 116, 920
TscAI CASTG 1 cut(s) 487
TseFI GTSAC 1 cut(s) 682
TseI GCWGC 1 cut(s) 150
Tsp45I GTSAC 1 cut(s) 682
TspDTI ATGAA 6 cut(s) 48, 80, 112, 176, 496, 685
TspGWI ACGGA 1 cut(s) 727
TspRI CASTG 1 cut(s) 487
Tth111I GACNNNGTC 1 cut(s) 680
VneI GTGCAC 1 cut(s) 386
VspI ATTAAT 1 cut(s) 116
XapI RAATTY 1 cut(s) 52
XbaI TCTAGA 1 cut(s) 56
XceI RCATGY 1 cut(s) 81
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 2 cut(s) 57, 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.