Rw2G006280

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006108
Physical Location & Seq
Reverse (-)
6065441 .. 6066385
945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006280.1

Sequence Viewer

Length: 909 bp
ATGGCCAGTGATATGGGGAATGTCAAATACGAAGAGGAGTACATCTTAAACTCTAGAGGAATGAAGCTTTTCACATGCAAATGGCTTCCGGACAATAACAAACCTCCCGAAGCCTTGATCTTCATCTGCCATGGATATGGCATGGAGTGTAGCATCACCATGAGCAGTACCGCAACCAGACTAGCCAAAGCAGGGTTTGCCATATACGGGATAGATTATGAAGGCCATGGAAGATCGTCAGGCCTTAAGGGCTATGTCAAGAGTTTTGATCGTGTTGTTGACGACTGCACCAACCACTTCACAAACATATGTGAGAGCAAAGAGAACAAAGGAAAAATGAGGTATCTGTTGGGGGAGTCAATGGGAGGAGCAGTGGCTCTGCTTGTTCATAGGAGAAAGCCACAATATTGGGATGATGATATGAGGCCATCTCCTATTGTGATCAGTCTTTTGAAAAAACTCTGCAGGGTTATCCCAACTTGGAAAATAATCCCCACAAATGATATCATCGATGTTGCTTTTAAAGTACCCGAGGTTAGGAAACAGACGAGAGAAAACCCTTACTTCTACAAAGGCCGACCTCGTCTGAAAACCGGCTACGAACTTTTGAGGGTCAGCACGGATATTGAACAGAGGCTTCAAGAGATCACATTGCCGTTCCTAGTTCTCCATGGCGAAGATGATAAAGTTACCGACACATCAGTTAGCAAACAGCTCTATGAGGTGGCCTCGAGTTCTGACAAGTCACTGAAGTTGTACCCGAATATGTGGCATGGTCTGCTCTATGGAGAACTAATTGAGAATACTGAGATTGTGTTTTCAGACATAATCAATTGGTTAGACAGAAGAAGCACCTCCGGAACTTCAAGGTCGGAGGGAGAGTTGAGAAGGGATGGTGAAAAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

302

Amino Acids

34.58

Weight (kDa)

8.1

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 35 - 261 3.7e-57 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 39 - 134 2.6e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 88, 857
AciI CCGC 1 cut(s) 171
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 770
AfaI GTAC 4 cut(s) 41, 169, 528, 758
AfiI CCNNNNNNNGG 3 cut(s) 192, 207, 537
AflII CTTAAG 1 cut(s) 245
AgsI TTSAA 4 cut(s) 454, 629, 641, 867
AluBI AGCT 2 cut(s) 67, 715
AluI AGCT 2 cut(s) 67, 715
Ama87I CYCGRG 2 cut(s) 530, 730
Aor13HI TCCGGA 2 cut(s) 88, 857
AoxI GGCC 6 cut(s) 3, 223, 241, 425, 574, 726
Asp700I GAANNNNTTC 1 cut(s) 68
AsuHPI GGTGA 2 cut(s) 148, 908
AvaI CYCGRG 2 cut(s) 530, 730
BalI TGGCCA 1 cut(s) 5
BccI CCATC 2 cut(s) 436, 887
BceAI ACGGC 1 cut(s) 640
BclI TGATCA 1 cut(s) 441
BfaI CTAG 3 cut(s) 54, 182, 662
BfmI CTRYAG 1 cut(s) 463
BfrI CTTAAG 1 cut(s) 245
BglI GCCNNNNNGGC 1 cut(s) 249
BmeT110I CYCGRG 2 cut(s) 530, 730
BmsI GCATC 1 cut(s) 162
BplI GAGNNNNNCTC 4 cut(s) 415, 447, 713, 745
Bsa29I ATCGAT 1 cut(s) 510
BsaBI GATNNNNATC 1 cut(s) 122
BsaJI CCNNGG 4 cut(s) 130, 226, 531, 670
BsaWI WCCGGW 2 cut(s) 88, 857
BsaXI ACNNNNNCTCC 4 cut(s) 357, 387, 866, 896
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 207, 537
Bse118I RCCGGY 1 cut(s) 593
Bse1I ACTGG 1 cut(s) 6
Bse3DI GCAATG 1 cut(s) 650
Bse8I GATNNNNATC 1 cut(s) 122
BseAI TCCGGA 2 cut(s) 88, 857
BseCI ATCGAT 1 cut(s) 510
BseDI CCNNGG 4 cut(s) 130, 226, 531, 670
BseGI GGATG 2 cut(s) 418, 898
BseJI GATNNNNATC 1 cut(s) 122
BseLI CCNNNNNNNGG 3 cut(s) 192, 207, 537
BseMI GCAATG 1 cut(s) 650
BseMII CTCAG 1 cut(s) 798
BseNI ACTGG 1 cut(s) 6
BseRI GAGGAG 2 cut(s) 50, 381
BsgI GTGCAG 1 cut(s) 271
BshFI GGCC 6 cut(s) 5, 225, 243, 427, 576, 728
BshVI ATCGAT 1 cut(s) 510
BsiHKCI CYCGRG 2 cut(s) 530, 730
BsiSI CCGG 3 cut(s) 89, 594, 858
BslI CCNNNNNNNGG 3 cut(s) 192, 207, 537
BsnI GGCC 6 cut(s) 5, 225, 243, 427, 576, 728
BsoBI CYCGRG 2 cut(s) 530, 730
Bsp13I TCCGGA 2 cut(s) 88, 857
Bsp143I GATC 5 cut(s) 117, 233, 268, 441, 645
Bsp19I CCATGG 3 cut(s) 130, 226, 670
BspACI CCGC 1 cut(s) 171
BspANI GGCC 6 cut(s) 5, 225, 243, 427, 576, 728
BspCNI CTCAG 1 cut(s) 799
BspDI ATCGAT 1 cut(s) 510
BspEI TCCGGA 2 cut(s) 88, 857
BspMAI CTGCAG 1 cut(s) 467
BspTI CTTAAG 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 650
BsrFI RCCGGY 1 cut(s) 593
BsrI ACTGG 1 cut(s) 6
BssAI RCCGGY 1 cut(s) 593
BssECI CCNNGG 4 cut(s) 130, 226, 531, 670
BssMI GATC 5 cut(s) 117, 233, 268, 441, 645
BssT1I CCWWGG 3 cut(s) 130, 226, 670
Bst6I CTCTTC 1 cut(s) 27
BstAFI CTTAAG 1 cut(s) 245
BstAPI GCANNNNNTGC 2 cut(s) 197, 778
BstDEI CTNAG 1 cut(s) 807
BstDSI CCRYGG 3 cut(s) 130, 226, 670
BstF5I GGATG 2 cut(s) 418, 898
BstKTI GATC 5 cut(s) 120, 236, 271, 444, 648
BstMBI GATC 5 cut(s) 117, 233, 268, 441, 645
BstMWI GCNNNNNNNGC 3 cut(s) 197, 249, 778
BstNSI RCATGY 1 cut(s) 78
BstSFI CTRYAG 1 cut(s) 463
BstXI CCANNNNNNTGG 3 cut(s) 13, 137, 408
Bsu15I ATCGAT 1 cut(s) 510
BsuRI GGCC 6 cut(s) 5, 225, 243, 427, 576, 728
BsuTUI ATCGAT 1 cut(s) 510
BtgI CCRYGG 3 cut(s) 130, 226, 670
BtsCI GGATG 2 cut(s) 418, 898
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 3 cut(s) 13, 378, 746
Cfr10I RCCGGY 1 cut(s) 593
ClaI ATCGAT 1 cut(s) 510
Csp6I GTAC 4 cut(s) 40, 168, 527, 757
CviAII CATG 7 cut(s) 75, 131, 142, 160, 227, 671, 773
CviQI GTAC 4 cut(s) 40, 168, 527, 757
DdeI CTNAG 1 cut(s) 807
DpnI GATC 5 cut(s) 119, 235, 270, 443, 647
DpnII GATC 5 cut(s) 117, 233, 268, 441, 645
DraI TTTAAA 1 cut(s) 523
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Eco130I CCWWGG 3 cut(s) 130, 226, 670
Eco147I AGGCCT 1 cut(s) 243
Eco32I GATATC 1 cut(s) 505
Eco57I CTGAAG 1 cut(s) 770
Eco88I CYCGRG 2 cut(s) 530, 730
EcoRV GATATC 1 cut(s) 505
EcoT14I CCWWGG 3 cut(s) 130, 226, 670
ErhI CCWWGG 3 cut(s) 130, 226, 670
FaeI CATG 7 cut(s) 78, 134, 145, 163, 230, 674, 776
FatI CATG 7 cut(s) 74, 130, 141, 159, 226, 670, 772
FauNDI CATATG 1 cut(s) 308
FbaI TGATCA 1 cut(s) 441
FokI GGATG 2 cut(s) 425, 905
FspBI CTAG 3 cut(s) 54, 182, 662
HaeIII GGCC 6 cut(s) 5, 225, 243, 427, 576, 728
HapII CCGG 3 cut(s) 89, 594, 858
Hin1II CATG 7 cut(s) 78, 134, 145, 163, 230, 674, 776
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 1 cut(s) 356
HpaII CCGG 3 cut(s) 89, 594, 858
HphI GGTGA 2 cut(s) 148, 908
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 4 cut(s) 588, 739, 823, 874
Hpy188III TCNNGA 6 cut(s) 54, 89, 107, 259, 641, 858
Hpy8I GTNNAC 1 cut(s) 280
HpyAV CCTTC 2 cut(s) 215, 882
HpyCH4V TGCA 3 cut(s) 78, 288, 465
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 249, 778
HpyF3I CTNAG 1 cut(s) 807
Hsp92II CATG 7 cut(s) 78, 134, 145, 163, 230, 674, 776
Kpn2I TCCGGA 2 cut(s) 88, 857
Ksp22I TGATCA 1 cut(s) 441
Kzo9I GATC 5 cut(s) 117, 233, 268, 441, 645
LmnI GCTCC 1 cut(s) 368
LpnPI CCDG 8 cut(s) 19, 102, 177, 190, 225, 451, 607, 871
LweI GCATC 1 cut(s) 162
MaeI CTAG 3 cut(s) 54, 182, 662
MaeIII GTNAC 2 cut(s) 688, 744
MalI GATC 5 cut(s) 119, 235, 270, 443, 647
MboI GATC 5 cut(s) 117, 233, 268, 441, 645
MboII GAAGA 5 cut(s) 44, 112, 243, 689, 858
MfeI CAATTG 1 cut(s) 832
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 795, 832
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 1 cut(s) 852
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 2 cut(s) 88, 857
MroXI GAANNNNTTC 1 cut(s) 68
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 47, 246, 522, 907
MslI CAYNNNNRTG 3 cut(s) 79, 135, 158
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 245
MspI CCGG 3 cut(s) 89, 594, 858
MunI CAATTG 1 cut(s) 832
MwoI GCNNNNNNNGC 3 cut(s) 197, 249, 778
NcoI CCATGG 3 cut(s) 130, 226, 670
NdeI CATATG 1 cut(s) 308
NdeII GATC 5 cut(s) 117, 233, 268, 441, 645
NlaIII CATG 7 cut(s) 78, 134, 145, 163, 230, 674, 776
NmuCI GTSAC 1 cut(s) 744
NspI RCATGY 1 cut(s) 78
PaeR7I CTCGAG 1 cut(s) 730
PceI AGGCCT 1 cut(s) 243
PdmI GAANNNNTTC 1 cut(s) 68
PflFI GACNNNGTC 1 cut(s) 582
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
PspXI VCTCGAGB 1 cut(s) 730
PstI CTGCAG 1 cut(s) 467
PsyI GACNNNGTC 1 cut(s) 582
RsaI GTAC 4 cut(s) 41, 169, 528, 758
RsaNI GTAC 4 cut(s) 40, 168, 527, 757
RseI CAYNNNNRTG 3 cut(s) 79, 135, 158
SaqAI TTAA 4 cut(s) 47, 246, 522, 907
Sau3AI GATC 5 cut(s) 117, 233, 268, 441, 645
SchI GAGTC 1 cut(s) 365
SetI ASST 9 cut(s) 69, 106, 344, 537, 583, 717, 726, 857, 872
SfaNI GCATC 1 cut(s) 162
SfcI CTRYAG 1 cut(s) 463
Sfr274I CTCGAG 1 cut(s) 730
SlaI CTCGAG 1 cut(s) 730
SmiMI CAYNNNNRTG 3 cut(s) 79, 135, 158
SmlI CTYRAG 2 cut(s) 245, 730
SmoI CTYRAG 2 cut(s) 245, 730
Sse9I AATT 2 cut(s) 795, 832
SseBI AGGCCT 1 cut(s) 243
SsiI CCGC 1 cut(s) 171
SspI AATATT 1 cut(s) 407
SspMI CTAG 3 cut(s) 54, 182, 662
StuI AGGCCT 1 cut(s) 243
StyI CCWWGG 3 cut(s) 130, 226, 670
TaqI TCGA 2 cut(s) 510, 731
TasI AATT 2 cut(s) 795, 832
TatI WGTACW 1 cut(s) 39
Tru1I TTAA 4 cut(s) 47, 246, 522, 907
Tru9I TTAA 4 cut(s) 47, 246, 522, 907
TscAI CASTG 3 cut(s) 13, 378, 753
TseFI GTSAC 1 cut(s) 744
Tsp45I GTSAC 1 cut(s) 744
TspDTI ATGAA 4 cut(s) 77, 112, 234, 377
TspGWI ACGGA 1 cut(s) 635
TspRI CASTG 3 cut(s) 13, 378, 753
Tth111I GACNNNGTC 1 cut(s) 582
Vha464I CTTAAG 1 cut(s) 245
XbaI TCTAGA 1 cut(s) 53
XceI RCATGY 1 cut(s) 78
XhoI CTCGAG 1 cut(s) 730
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 3 cut(s) 54, 182, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.