Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0001743

Physical Location & Seq

Genomic Coordinates Forward (+)

1864592 .. 1871001

Gene Structure 6410 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0023500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3195 bp

ATGCCGGAGGGTACCAGAATGGCCGGGAACGACTGGATAAACAGTTACCTGGAGGCGATTCTCGACGTCGGTCCCGGCCTCGTCGACGATGCTAAGTCGTCGTTGCTGCTTCGGGAGAGAGGTCACTTCAGTCCGACGAGGTACTTCGTGGAGGAGGTCATCACCGGCTTCGATGAGACCGATCTCCACCGCTCTTGGGTTCGCGCCGCGGCGACGAGGAGTCCGCAGGAGAGGAACACCAGGTTGGAGAATCTGTGCTGGCGGATTTGGAACTTGGCTCGCCAGAAGAAGCAGCTTGAGGAAAAGGAAGCTCAGAGGATGACTAAGCGTCGTCTTGAAAGAGAAAGGGGCCGCAGAGAAGCTACTGCTGATATGTCGGAGGACTTATCTGAGGGAGAGAAGGGAGATGTAGTGGCGTCTGATGTATCGGCTCATGGTGATAGTGTCAGAGGCAGACTGCCGAGGATTAACTCTGTTGACGCAATGGAGACTTTTATAAGTCAGCAGAAGGGGAAGAAGCTATATATCGTCTTAATAAGTCTTCATGGTCTTATCCGGGGTGAAAATATGGAGCTCGGTCGCGACTCTGATACTGGTGGTCAGGTTAAATATGTAGTGGAACTTGCAAGGGCTTTGGGTAGCATGCCAGGAGTGTATCGAGTTGATCTGCTAACTAGACAAGTGTCATCACCTGACGTAGATTGGAGTTATGGTGAGCCCACAGAGATGCTAACTCCACTAAGTGCAGAAGGTTTCGAGGAAGAGACAGGAGAGAGCAGTGGTTCTTACATCATTCGTATACCATTTGGTCCCAGAGATAAATACATCCCCAAAGAAAACCTCTGGCCACACATCCCCGAATTTGTTGATGGTGCATTGAACCACGTAATACAAATGTCCAAAGTTTTAGGTGAACAAGTTGGTGGTGGGAAGCCAGTTTGGCCAGTTGCCATCCATGGGCATTATGCAGATGCAGGTGACTCTGCTGCCCTCCTATCTGGTGCTTTAAATGTACCGATGCTTTTCACTGGCCACTCACTTGGCAGAGATAAGTTGGAGCAACTTTTAAAACAAGGCCGTCTATCAAGGGATGAAATAAACGCCACATACAAAATAATGCGTCGGATAGAAGCTGAGGAGTTATCCCTTGATGCCTCTGAAATAGTTATTACCAGCACTAGACAGGAGATTGACGAGCAATGGCGCTTGTATGATGGATTCGATCCCATACTGGAGCGAAAAATACGAGCCAGGATCAGGCGTAATGTGAGCTGTTATGGCAGGTTCATGCCCCGCATGGTGGTAATTCCTCCTGGAATGGAGTTCCATCATATTGTTCCACAGGATGGTGATATGGATGGTGAAACAGAGGCAAACGAAGACCATCATCCTACTCCTGCAGACCCACCTATTTGGACTGAGATAATGCGCTTCTTTACCAATCCTCGAAAGCCTATGATTCTTGCCCTTGCTAGACCAGATCCAAAAAAGAACATCACAACTTTGGTCAAAGCATTTGGAGAATGTCGTCCATTAAGAGAGCTTGCTAATCTTACATTAATTATGGGTAATCGAGATGGAATTGATGACATGTCAAGCACAAGTGCTTCTGTTCTTCTTTCAGTTCTCAAGCTCATTGACAAACATGATTTGTATGGGCAAGTTGCATACCCCAAACACCACAAGCAGTCTGATGTTCCTGAGATATATCGTCTTGCAGCAAAGACAAAGGGTGTTTTCATCAATCCAGCTTTCATCGAGCCATTCGGACTAACATTGATTGAGGCAGCTGCTTATGGTTTACCTATTGTTGCCACAAAAAATGGCGGTCCTGTTGATATTATACAGGTCCTTGACAATGGTCTTCTTATTGATCCCCATGATCAGCAGTCTATCGCCGATGCTCTTCTGAAGCTTGTTGCAGATAAGCAGCTCTGGGCAAGATGTCGTCAGAATGGATTGAAGAACATTCACCTATACTCATGGCCTGAGCACTGCAAGACTTACCTATCTCGAATAGCCAGCTGCAAAACAAGGCATCCACAGTGGCAAAGAAGTGATGATGGAGCAGAATCTTCAGAATCGGATTCCCCAAGTGATTCCTTGAGGGATATACAGGATTTGTCTTTGAACTTGAAGTTCTCAATGGATGGAGAAAGGAGTGGAATTAGTGTGAATGAGAACTCTTTAGAATCTGAAGGAAGTATTGATAGAAAGAGTAAAATAGAGAATGCTGTTTTGGCATGGTCAAAGGGTATTTCAAGGGACACGCGGAAAGCTGGTTTCTCAGAGAAAGCAGACCATAACAGTAGTGGTAAGTTCCCAGTGTTGAGGAGGAGGAAACATCTTATTGTCATTGCTGTGGATTGTGATACCATTACAGATCTGATCGAAACCACTAAAAAGATTTTTGAGGCTACAGAAAAGGAAAGGACAGAAGGATCAATAGGGTACATATTGTCAACATCCTTGACCATAATCGAGATACGCTCGTTTCTGGTCTCTGGGGGCTTGGGCCCTAATGATTTTGATGCGTTTATTTGCAACAGTGGCAGTGATCTCTATTATCCATCTATTAATTCAGATGATCGTCCATTTGTGGTTGACTTTTATTACCACTCGCACATTGAATATCGCTGGGGTGGAGAAGGGTTGAGGAAGACGTTGGTACGCTGGGCAACTTCAACCAATGATAAGAAGAGTGGGAGTGAGGAACAGATTGTTACTGCATCTGAACAACTTTCAACCGACTATTGTTATGCTTTTAAAGTGCAAAAGCCAGCATTGGTTCCCCCTGTGAAGGAGCTTCGGAAGTTGCTGAGGATTCAAGCTTTGCGGTGCCATGTAATTTACTGTCAAAATGGTAACAGACTGAATGTGATTCCAGTGACTGCATCTCGTTCCCAAGCCCTCAGGTATTTATATTTAAGATGGGGTGTAGAGTTATCAAAGATGGTGGTTGTTGCTGGAGAATGCGGAGACACAGATTATGAAGGGTTGCTTGGAGGGCTGCACAAAAGTGTAGTACTGAAGGGGGTCGGCAGCAATGCAATCAGTCAACTCCACACGAACCGAAACTACCCTCTGTCAGATGTCCTAGCGTTTGACAGCCCCAACATTGTTCAGACAACTGAAGGTTGTGGCAGCGATGATATCCGCGGTGCCTTGGAGAAATTAGAGGTTGTCAAGAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

1064

Amino Acids

119.18

Weight (kDa)

6.05

Isoelectric Point (pI)

45.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GT-B_Sucrose_synth	PF00862	162 - 440	2e-10	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	480 - 654	1.3e-24	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	485 - 642	1.2e-15	Glycosyl transferases group 1
S6PP	PF05116	844 - 1010	2e-12	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001743)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77340 AT5G65560 AT5G65560 AT5G65560
fragaria_vesca	FvH4_1g06430 FvH4_2g11090 FvH4_2g11090 FvH4_2g11090 FvH4_2g11090 FvH4_2g11090 FvH4_7g04271 FvH4_7g04271
malus_domestica	MD03G1170600.v1.1 MD08G1245900.v1.1 MD11G1188000.v1.1 MD15G1441200.v1.1
prunus_persica	Prupe.1G583300_v2.0.a1 Prupe.4G256300_v2.0.a1
pyrus_communis	pycom03g12750 pycom08g21340 pycom15g38890 pycom15g38910
rosa_chinensis	RchiOBHm_Chr1g0329241 RchiOBHm_Chr2g0092171
rosa_laevigata	RLG00000016282 RLG00000029907
rosa_multiflora	Rmu_co8315275.1_g000001 Rmu_sc0000203.1_g000005 Rmu_sc0000524.1_g000014 Rmu_sc0010272.1_g000004 Rmu_sc0013552.1_g000009
rosa_roxburghii	Rroxscaffold_2G00149410 Rroxscaffold_4G00321300
rosa_rugosa	Rorug01G0077300 Rorug01G0077300 Rorug02G0023500
rosa_samantha	Rh1AG096400 Rh1BG077100 Rh1CG093100 Rh1CG107900 Rh1DG099700 Rh1DG116600 Rh2AG069100 Rh2BG068600 Rh2CG069700 Rh2DG067700
rosa_wichuraiana	Rw1G007460 Rw2G006310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	497
AarI	CACCTGC	1 cut(s)	965
AatII	GACGTC	1 cut(s)	69
Acc36I	ACCTGC	2 cut(s)	965, 1272
Acc65I	GGTACC	1 cut(s)	11
AccB1I	GGYRCC	3 cut(s)	11, 2838, 3161
AccB7I	CCANNNNNTGG	1 cut(s)	1346
AccBSI	CCGCTC	1 cut(s)	192
AccI	GTMKAC	2 cut(s)	84, 799
AccII	CGCG	5 cut(s)	204, 209, 582, 2272, 3159
AclWI	GGATC	5 cut(s)	1217, 1262, 1475, 1868, 2449
AcoI	YGGCCR	4 cut(s)	21, 845, 941, 1030
AcsI	RAATTY	1 cut(s)	860
AcuI	CTGAAG	6 cut(s)	112, 1931, 2061, 2217, 3050, 3153
AcyI	GRCGYC	2 cut(s)	66, 416
AdeI	CACNNNGTG	1 cut(s)	743
AfaI	GTAC	6 cut(s)	13, 143, 1014, 2453, 2670, 3027
AfiI	CCNNNNNNNGG	6 cut(s)	196, 957, 1257, 1300, 1346, 2800
AflIII	ACRYGT	1 cut(s)	1590
AhdI	GACNNNNNGTC	2 cut(s)	219, 327
AjnI	CCWGG	5 cut(s)	48, 239, 646, 1250, 1312
AjuI	GAANNNNNNNTTGG	6 cut(s)	227, 259, 2222, 2254, 2985, 3017
AleI	CACNNNNGTG	1 cut(s)	3018
Alw21I	GWGCWC	2 cut(s)	576, 1995
Alw26I	GTCTC	5 cut(s)	170, 482, 758, 2506, 2973
AlwI	GGATC	5 cut(s)	1217, 1262, 1475, 1868, 2449
AlwNI	CAGNNNCTG	1 cut(s)	2891
AoxI	GGCC	9 cut(s)	21, 76, 349, 845, 941, 1030, 1075, 1985, 2515
ApaI	GGGCCC	1 cut(s)	2519
ApoI	RAATTY	1 cut(s)	860
AseI	ATTAAT	2 cut(s)	1559, 2577
Asp718I	GGTACC	1 cut(s)	11
AspLEI	GCGC	3 cut(s)	206, 1206, 1431
AspS9I	GGNCC	7 cut(s)	71, 349, 809, 1829, 1849, 2515, 2516
AsuC2I	CCSGG	3 cut(s)	25, 75, 557
AvaII	GGWCC	4 cut(s)	71, 809, 1829, 1849
AxyI	CCTNAGG	1 cut(s)	2912
BaeGI	GKGCMC	1 cut(s)	2519
BaeI	ACNNNNGTAYC	2 cut(s)	2477, 2510
BalI	TGGCCA	3 cut(s)	847, 943, 1032
BanI	GGYRCC	3 cut(s)	11, 2838, 3161
BanII	GRGCYC	3 cut(s)	576, 720, 2519
BbsI	GAAGAC	4 cut(s)	533, 1386, 1856, 2666
Bbv12I	GWGCWC	2 cut(s)	576, 1995
BbvCI	CCTCAGC	2 cut(s)	1134, 2819
BceAI	ACGGC	1 cut(s)	1062
BciT130I	CCWGG	5 cut(s)	50, 241, 648, 1252, 1314
BclI	TGATCA	1 cut(s)	1882
BcnI	CCSGG	3 cut(s)	25, 75, 557
BcoDI	GTCTC	5 cut(s)	170, 482, 758, 2506, 2973
BfaI	CTAG	5 cut(s)	675, 1179, 1473, 3098, 3193
BfmI	CTRYAG	2 cut(s)	1398, 2418
BfoI	RGCGCY	1 cut(s)	1207
BfuAI	ACCTGC	2 cut(s)	965, 1272
BglI	GCCNNNNNGGC	1 cut(s)	940
BglII	AGATCT	1 cut(s)	2383
BmcAI	AGTACT	1 cut(s)	3027
Bme1390I	CCNGG	8 cut(s)	25, 50, 75, 241, 557, 648, 1252, 1314
Bme18I	GGWCC	4 cut(s)	71, 809, 1829, 1849
BmeRI	GACNNNNNGTC	2 cut(s)	219, 327
BmgT120I	GGNCC	7 cut(s)	71, 349, 809, 1829, 1849, 2515, 2516
BmiI	GGNNCC	8 cut(s)	13, 73, 350, 811, 2517, 2790, 2840, 3163
BmrFI	CCNGG	8 cut(s)	25, 50, 75, 241, 557, 648, 1252, 1314
BmrI	ACTGGG	1 cut(s)	2318
BmuI	ACTGGG	1 cut(s)	2318
BoxI	GACNNNNGTC	3 cut(s)	69, 682, 1860
BpiI	GAAGAC	4 cut(s)	533, 1386, 1856, 2666
BplI	GAGNNNNNCTC	2 cut(s)	2474, 2506
BpmI	CTGGAG	3 cut(s)	71, 1253, 2988
Bpu10I	CCTNAGC	3 cut(s)	1134, 1989, 2819
BpuEI	CTTGAG	3 cut(s)	317, 1613, 2125
BpuMI	CCSGG	3 cut(s)	25, 75, 557
BsaAI	YACGTR	1 cut(s)	886
BsaHI	GRCGYC	2 cut(s)	66, 416
BsaI	GGTCTC	2 cut(s)	170, 2506
BsaJI	CCNNGG	6 cut(s)	207, 461, 556, 955, 3157, 3165
BsaXI	ACNNNNNCTCC	6 cut(s)	239, 269, 396, 426, 697, 727
Bsc4I	CCNNNNNNNGG	6 cut(s)	196, 957, 1257, 1300, 1346, 2800
Bse118I	RCCGGY	1 cut(s)	164
Bse1I	ACTGG	8 cut(s)	38, 598, 935, 944, 1033, 1236, 2324, 2885
Bse21I	CCTNAGG	1 cut(s)	2912
Bse3DI	GCAATG	4 cut(s)	489, 1205, 2355, 3052
BseBI	CCWGG	5 cut(s)	50, 241, 648, 1252, 1314
BseDI	CCNNGG	6 cut(s)	207, 461, 556, 955, 3157, 3165
BseLI	CCNNNNNNNGG	6 cut(s)	196, 957, 1257, 1300, 1346, 2800
BseMI	GCAATG	4 cut(s)	489, 1205, 2355, 3052
BseMII	CTCAG	9 cut(s)	326, 381, 1125, 1410, 1692, 1980, 2301, 2810, 2926
BseNI	ACTGG	8 cut(s)	38, 598, 935, 944, 1033, 1236, 2324, 2885
BseRI	GAGGAG	5 cut(s)	167, 232, 1151, 2347, 2350
BseSI	GKGCMC	1 cut(s)	2519
BseYI	CCCAGC	2 cut(s)	2637, 2673
BsgI	GTGCAG	2 cut(s)	765, 2996
Bsh1236I	CGCG	5 cut(s)	204, 209, 582, 2272, 3159
Bsh1285I	CGRYCG	1 cut(s)	580
BshFI	GGCC	9 cut(s)	23, 78, 351, 847, 943, 1032, 1077, 1987, 2517
BshNI	GGYRCC	3 cut(s)	11, 2838, 3161
BsiEI	CGRYCG	1 cut(s)	580
BsiHKAI	GWGCWC	2 cut(s)	576, 1995
BsiSI	CCGG	5 cut(s)	5, 24, 75, 165, 556
BslFI	GGGAC	3 cut(s)	57, 795, 2279
BslI	CCNNNNNNNGG	6 cut(s)	196, 957, 1257, 1300, 1346, 2800
BsmAI	GTCTC	5 cut(s)	170, 482, 758, 2506, 2973
BsmFI	GGGAC	3 cut(s)	57, 795, 2279
BsmI	GAATGC	2 cut(s)	2236, 2978
BsnI	GGCC	9 cut(s)	23, 78, 351, 847, 943, 1032, 1077, 1987, 2517
Bso31I	GGTCTC	2 cut(s)	170, 2506
Bsp120I	GGGCCC	1 cut(s)	2515
Bsp1286I	GDGCHC	4 cut(s)	576, 720, 1995, 2519
Bsp19I	CCATGG	1 cut(s)	955
Bsp68I	TCGCGA	1 cut(s)	582
BspANI	GGCC	9 cut(s)	23, 78, 351, 847, 943, 1032, 1077, 1987, 2517
BspCNI	CTCAG	9 cut(s)	325, 382, 1126, 1411, 1693, 1981, 2300, 2811, 2925
BspFNI	CGCG	5 cut(s)	204, 209, 582, 2272, 3159
BspLI	GGNNCC	8 cut(s)	13, 73, 350, 811, 2517, 2790, 2840, 3163
BspMAI	CTGCAG	1 cut(s)	1402
BspMI	ACCTGC	2 cut(s)	965, 1272
BspPI	GGATC	5 cut(s)	1217, 1262, 1475, 1868, 2449
BspQI	GCTCTTC	1 cut(s)	1911
BspT107I	GGYRCC	3 cut(s)	11, 2838, 3161
BspTNI	GGTCTC	2 cut(s)	170, 2506
BsrBI	CCGCTC	1 cut(s)	192
BsrDI	GCAATG	4 cut(s)	489, 1205, 2355, 3052
BsrFI	RCCGGY	1 cut(s)	164
BsrI	ACTGG	8 cut(s)	38, 598, 935, 944, 1033, 1236, 2324, 2885
BssAI	RCCGGY	1 cut(s)	164
BssECI	CCNNGG	6 cut(s)	207, 461, 556, 955, 3157, 3165
BssNAI	GTATAC	1 cut(s)	800
BssNI	GRCGYC	2 cut(s)	66, 416
BssT1I	CCWWGG	2 cut(s)	955, 3165
Bst1107I	GTATAC	1 cut(s)	800
Bst2UI	CCWGG	5 cut(s)	50, 241, 648, 1252, 1314
Bst4CI	ACNGT	5 cut(s)	44, 2046, 2309, 2549, 2855
Bst6I	CTCTTC	3 cut(s)	756, 1911, 2693
BstACI	GRCGYC	2 cut(s)	66, 416
BstBAI	YACGTR	1 cut(s)	886
BstC8I	GCNNGC	6 cut(s)	260, 280, 644, 1545, 2023, 2781
BstDSI	CCRYGG	3 cut(s)	207, 955, 3157
BstFNI	CGCG	5 cut(s)	204, 209, 582, 2272, 3159
BstH2I	RGCGCY	1 cut(s)	1207
BstHHI	GCGC	3 cut(s)	206, 1206, 1431
BstMAI	GTCTC	5 cut(s)	170, 482, 758, 2506, 2973
BstMCI	CGRYCG	1 cut(s)	580
BstMWI	GCNNNNNNNGC	5 cut(s)	940, 1278, 2240, 2550, 3007
BstNI	CCWGG	5 cut(s)	50, 241, 648, 1252, 1314
BstNSI	RCATGY	2 cut(s)	646, 1594
BstPAI	GACNNNNGTC	3 cut(s)	69, 682, 1860
BstSCI	CCNGG	8 cut(s)	23, 48, 73, 239, 555, 646, 1250, 1312
BstSFI	CTRYAG	2 cut(s)	1398, 2418
BstSLI	GKGCMC	1 cut(s)	2519
BstUI	CGCG	5 cut(s)	204, 209, 582, 2272, 3159
BstV2I	GAAGAC	4 cut(s)	533, 1386, 1856, 2666
BstX2I	RGATCY	2 cut(s)	1480, 2383
BstXI	CCANNNNNNTGG	2 cut(s)	1040, 1413
BstYI	RGATCY	2 cut(s)	1480, 2383
BstZ17I	GTATAC	1 cut(s)	800
Bsu36I	CCTNAGG	1 cut(s)	2912
BsuRI	GGCC	9 cut(s)	23, 78, 351, 847, 943, 1032, 1077, 1987, 2517
BtgI	CCRYGG	3 cut(s)	207, 955, 3157
BtgZI	GCGATG	1 cut(s)	3162
BtsI	GCAGTG	3 cut(s)	784, 1993, 2560
BtsIMutI	CAGTG	8 cut(s)	784, 1026, 1993, 2051, 2331, 2554, 2560, 2892
BtuMI	TCGCGA	1 cut(s)	582
BveI	ACCTGC	2 cut(s)	965, 1272
Cac8I	GCNNGC	6 cut(s)	260, 280, 644, 1545, 2023, 2781
CaiI	CAGNNNCTG	1 cut(s)	2891
CfoI	GCGC	3 cut(s)	206, 1206, 1431
Cfr10I	RCCGGY	1 cut(s)	164
Cfr13I	GGNCC	7 cut(s)	71, 349, 809, 1829, 1849, 2515, 2516
Cfr42I	CCGCGG	2 cut(s)	210, 3160
CseI	GACGC	4 cut(s)	317, 405, 488, 1109
CsiI	ACCWGGT	1 cut(s)	239
Csp6I	GTAC	6 cut(s)	12, 142, 1013, 2452, 2669, 3026
CspCI	CAANNNNNGTGG	4 cut(s)	176, 211, 2937, 2972
CviQI	GTAC	6 cut(s)	12, 142, 1013, 2452, 2669, 3026
DraI	TTTAAA	3 cut(s)	1008, 1068, 2767
DraIII	CACNNNGTG	1 cut(s)	743
DriI	GACNNNNNGTC	2 cut(s)	219, 327
EaeI	YGGCCR	4 cut(s)	21, 845, 941, 1030
Eam1104I	CTCTTC	3 cut(s)	756, 1911, 2693
Eam1105I	GACNNNNNGTC	2 cut(s)	219, 327
EarI	CTCTTC	3 cut(s)	756, 1911, 2693
EciI	GGCGGA	1 cut(s)	277
Ecl136II	GAGCTC	1 cut(s)	574
Eco130I	CCWWGG	2 cut(s)	955, 3165
Eco24I	GRGCYC	3 cut(s)	576, 720, 2519
Eco31I	GGTCTC	2 cut(s)	170, 2506
Eco32I	GATATC	1 cut(s)	3154
Eco47I	GGWCC	4 cut(s)	71, 809, 1829, 1849
Eco53kI	GAGCTC	1 cut(s)	574
Eco57I	CTGAAG	6 cut(s)	112, 1931, 2061, 2217, 3050, 3153
Eco81I	CCTNAGG	1 cut(s)	2912
EcoICRI	GAGCTC	1 cut(s)	574
EcoO109I	RGGNCCY	2 cut(s)	1849, 2516
EcoRII	CCWGG	5 cut(s)	48, 239, 646, 1250, 1312
EcoRV	GATATC	1 cut(s)	3154
EcoT14I	CCWWGG	2 cut(s)	955, 3165
EcoT38I	GRGCYC	3 cut(s)	576, 720, 2519
ErhI	CCWWGG	2 cut(s)	955, 3165
FalI	AAGNNNNNCTT	2 cut(s)	2985, 3017
FaqI	GGGAC	3 cut(s)	57, 795, 2279
FauI	CCCGC	1 cut(s)	1301
FbaI	TGATCA	1 cut(s)	1882
FblI	GTMKAC	2 cut(s)	84, 799
FriOI	GRGCYC	3 cut(s)	576, 720, 2519
FspBI	CTAG	5 cut(s)	675, 1179, 1473, 3098, 3193
GlaI	GCGC	3 cut(s)	205, 1205, 1430
GsaI	CCCAGC	2 cut(s)	2641, 2677
GsuI	CTGGAG	3 cut(s)	71, 1253, 2988
HaeII	RGCGCY	1 cut(s)	1207
HaeIII	GGCC	9 cut(s)	23, 78, 351, 847, 943, 1032, 1077, 1987, 2517
HapII	CCGG	5 cut(s)	5, 24, 75, 165, 556
HgaI	GACGC	4 cut(s)	317, 405, 488, 1109
HhaI	GCGC	3 cut(s)	206, 1206, 1431
Hin1I	GRCGYC	2 cut(s)	66, 416
Hin6I	GCGC	3 cut(s)	204, 1204, 1429
HinP1I	GCGC	3 cut(s)	204, 1204, 1429
HincII	GTYRAC	5 cut(s)	85, 478, 2463, 2605, 3059
HindII	GTYRAC	5 cut(s)	85, 478, 2463, 2605, 3059
HindIII	AAGCTT	2 cut(s)	1913, 2829
HpaII	CCGG	5 cut(s)	5, 24, 75, 165, 556
Hpy166II	GTNNAC	8 cut(s)	85, 478, 800, 914, 1802, 2463, 2605, 3059
Hpy188III	TCNNGA	9 cut(s)	62, 113, 335, 581, 1574, 1700, 2013, 2482, 3187
Hpy8I	GTNNAC	8 cut(s)	85, 478, 800, 914, 1802, 2463, 2605, 3059
Hpy99I	CGWCG	9 cut(s)	68, 71, 86, 89, 103, 139, 217, 333, 1125
HpyCH4III	ACNGT	5 cut(s)	44, 2046, 2309, 2549, 2855
HpyCH4IV	ACGT	4 cut(s)	66, 696, 885, 2663
HpyF10VI	GCNNNNNNNGC	5 cut(s)	940, 1278, 2240, 2550, 3007
HpySE526I	ACGT	4 cut(s)	66, 696, 885, 2663
Hsp92I	GRCGYC	2 cut(s)	66, 416
HspAI	GCGC	3 cut(s)	204, 1204, 1429
KpnI	GGTACC	1 cut(s)	15
Ksp22I	TGATCA	1 cut(s)	1882
KspI	CCGCGG	2 cut(s)	210, 3160
LguI	GCTCTTC	1 cut(s)	1911
LmnI	GCTCC	5 cut(s)	571, 1057, 1234, 2066, 2803
MabI	ACCWGGT	1 cut(s)	239
MaeI	CTAG	5 cut(s)	675, 1179, 1473, 3098, 3193
MaeII	ACGT	4 cut(s)	66, 696, 885, 2663
MaeIII	GTNAC	6 cut(s)	44, 122, 977, 2722, 2864, 2887
MbiI	CCGCTC	1 cut(s)	192
MflI	RGATCY	2 cut(s)	1480, 2383
MhlI	GDGCHC	4 cut(s)	576, 720, 1995, 2519
MlsI	TGGCCA	3 cut(s)	847, 943, 1032
MluCI	AATT	8 cut(s)	860, 1305, 1560, 1581, 2166, 2578, 2847, 3173
MluNI	TGGCCA	3 cut(s)	847, 943, 1032
MlyI	GAGTC	3 cut(s)	229, 578, 974
MmeI	TCCRAC	5 cut(s)	158, 225, 357, 1035, 1103
Mox20I	TGGCCA	3 cut(s)	847, 943, 1032
MscI	TGGCCA	3 cut(s)	847, 943, 1032
MslI	CAYNNNNRTG	3 cut(s)	725, 2360, 3018
Msp20I	TGGCCA	3 cut(s)	847, 943, 1032
MspA1I	CMGCKG	4 cut(s)	209, 1790, 2025, 3159
MspI	CCGG	5 cut(s)	5, 24, 75, 165, 556
MspR9I	CCNGG	8 cut(s)	25, 50, 75, 241, 557, 648, 1252, 1314
Mva1269I	GAATGC	2 cut(s)	2236, 2978
MvaI	CCWGG	5 cut(s)	50, 241, 648, 1252, 1314
MvnI	CGCG	5 cut(s)	204, 209, 582, 2272, 3159
MwoI	GCNNNNNNNGC	5 cut(s)	940, 1278, 2240, 2550, 3007
NciI	CCSGG	3 cut(s)	25, 75, 557
NcoI	CCATGG	1 cut(s)	955
NlaIV	GGNNCC	8 cut(s)	13, 73, 350, 811, 2517, 2790, 2840, 3163
NmeAIII	GCCGAG	1 cut(s)	486
NmuCI	GTSAC	3 cut(s)	122, 977, 2887
NruI	TCGCGA	1 cut(s)	582
NspI	RCATGY	2 cut(s)	646, 1594
OliI	CACNNNNGTG	1 cut(s)	3018
PaeI	GCATGC	1 cut(s)	646
PaqCI	CACCTGC	1 cut(s)	965
PciI	ACATGT	1 cut(s)	1590
PciSI	GCTCTTC	1 cut(s)	1911
PcsI	WCGNNNNNNNCGW	1 cut(s)	177
PctI	GAATGC	2 cut(s)	2236, 2978
PflMI	CCANNNNNTGG	1 cut(s)	1346
PfoI	TCCNGGA	1 cut(s)	1312
PleI	GAGTC	3 cut(s)	228, 578, 974
PpsI	GAGTC	3 cut(s)	228, 578, 974
Ppu21I	YACGTR	1 cut(s)	886
PpuMI	RGGWCCY	1 cut(s)	1849
PscI	ACATGT	1 cut(s)	1590
PshAI	GACNNNNGTC	3 cut(s)	69, 682, 1860
PshBI	ATTAAT	2 cut(s)	1559, 2577
PsiI	TTATAA	1 cut(s)	497
Psp124BI	GAGCTC	1 cut(s)	576
Psp5II	RGGWCCY	1 cut(s)	1849
Psp6I	CCWGG	5 cut(s)	48, 239, 646, 1250, 1312
PspFI	CCCAGC	2 cut(s)	2637, 2673
PspGI	CCWGG	5 cut(s)	48, 239, 646, 1250, 1312
PspN4I	GGNNCC	8 cut(s)	13, 73, 350, 811, 2517, 2790, 2840, 3163
PspOMI	GGGCCC	1 cut(s)	2515
PspPI	GGNCC	7 cut(s)	71, 349, 809, 1829, 1849, 2515, 2516
PspPPI	RGGWCCY	1 cut(s)	1849
PsrI	GAACNNNNNNTAC	2 cut(s)	3062, 3094
PstI	CTGCAG	1 cut(s)	1402
PstNI	CAGNNNCTG	1 cut(s)	2891
PsuI	RGATCY	2 cut(s)	1480, 2383
PvuII	CAGCTG	2 cut(s)	1790, 2025
RruI	TCGCGA	1 cut(s)	582
RsaI	GTAC	6 cut(s)	13, 143, 1014, 2453, 2670, 3027
RsaNI	GTAC	6 cut(s)	12, 142, 1013, 2452, 2669, 3026
RseI	CAYNNNNRTG	3 cut(s)	725, 2360, 3018
SacI	GAGCTC	1 cut(s)	576
SacII	CCGCGG	2 cut(s)	210, 3160
SalI	GTCGAC	1 cut(s)	83
SapI	GCTCTTC	1 cut(s)	1911
Sau96I	GGNCC	7 cut(s)	71, 349, 809, 1829, 1849, 2515, 2516
ScaI	AGTACT	1 cut(s)	3027
SchI	GAGTC	3 cut(s)	229, 578, 974
ScrFI	CCNGG	8 cut(s)	25, 50, 75, 241, 557, 648, 1252, 1314
SduI	GDGCHC	4 cut(s)	576, 720, 1995, 2519
SexAI	ACCWGGT	1 cut(s)	239
SfcI	CTRYAG	2 cut(s)	1398, 2418
Sfr303I	CCGCGG	2 cut(s)	210, 3160
SgrBI	CCGCGG	2 cut(s)	210, 3160
SgrDI	CGTCGACG	1 cut(s)	83
SinI	GGWCC	4 cut(s)	71, 809, 1829, 1849
SmiMI	CAYNNNNRTG	3 cut(s)	725, 2360, 3018
SmlI	CTYRAG	3 cut(s)	296, 1628, 2104
SmoI	CTYRAG	3 cut(s)	296, 1628, 2104
SphI	GCATGC	1 cut(s)	646
Sse9I	AATT	8 cut(s)	860, 1305, 1560, 1581, 2166, 2578, 2847, 3173
SspMI	CTAG	5 cut(s)	675, 1179, 1473, 3098, 3193
SstI	GAGCTC	1 cut(s)	576
StyD4I	CCNGG	8 cut(s)	23, 48, 73, 239, 555, 646, 1250, 1312
StyI	CCWWGG	2 cut(s)	955, 3165
TaaI	ACNGT	5 cut(s)	44, 2046, 2309, 2549, 2855
TaiI	ACGT	4 cut(s)	69, 699, 888, 2666
TaqII	GACCGA	3 cut(s)	59, 194, 566
TasI	AATT	8 cut(s)	860, 1305, 1560, 1581, 2166, 2578, 2847, 3173
TatI	WGTACW	1 cut(s)	3025
TauI	GCSGC	3 cut(s)	209, 212, 354
TscAI	CASTG	8 cut(s)	784, 1033, 2000, 2051, 2331, 2554, 2560, 2892
TseFI	GTSAC	3 cut(s)	122, 977, 2887
Tsp45I	GTSAC	3 cut(s)	122, 977, 2887
TspDTI	ATGAA	6 cut(s)	533, 1107, 1276, 1729, 1744, 3006
TspRI	CASTG	8 cut(s)	784, 1033, 2000, 2051, 2331, 2554, 2560, 2892
Van91I	CCANNNNNTGG	1 cut(s)	1346
VpaK11BI	GGWCC	4 cut(s)	71, 809, 1829, 1849
VspI	ATTAAT	2 cut(s)	1559, 2577
XapI	RAATTY	1 cut(s)	860
XceI	RCATGY	2 cut(s)	646, 1594
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2309
XmiI	GTMKAC	2 cut(s)	84, 799
XspI	CTAG	5 cut(s)	675, 1179, 1473, 3098, 3193
ZraI	GACGTC	1 cut(s)	67
ZrmI	AGTACT	1 cut(s)	3027

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.