Rh2BG068600

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001743
Physical Location & Seq
Forward (+)
5184055 .. 5186832
2778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG068600.1

Sequence Viewer

Length: 1989 bp
ATGGGGCATTGTAGGAATAAGGATGTGGATAGTGCATACAGGACTTTTAAGGTGATCCCCAACAAGGGTTGTGCAAGAGATGTGGTTTCGTATAATGTATTGATACACGGGCTCTGTGAGGCTGGCAGGAGTAACGAGGCGTTTGAGTTGTACTCTAATATGGAGAAGGATGAGTGTTTACCAGATGCGCCGACGTATAATGTTCTTATTCATGCATTGTGTAGAGGGGGGAGGAGATTCGAAGGAATGAGTTTGTTTAAGGAGATGAAAGAGAAGGGTTGTGAGCCTAGTGTAAATACTTATAATGTGCTCATTACTAGCCTGTGTGACGAAAATAAGCTTCAGTATGCTAGGAAGGTTCTAGACCTGATGTCGGGGAAAGGGTTGGTTCCGGATGTTGTCACGTACAATGCATTGATTGATGGGTATTGCAAGGAGGGAGCGGTTGAGGCTGCGTTTGGGATTCTGGGTTTGATGGAATCTAATAAGTGTCGTCCAAATGCTCGCACGTTTACTGAATTGATTCATGGCTTTTGTAAAAGGAATAATGTGCACCAGGCAATGGCGCTTCTTGATAGGATGCTTGAACGGAAGCTCTCACCAGACCAGATTACATATAATTCGTTGATCAATGGGCAGTGTAAAGCAGGTCATGTAGATAGTGCTTATAGGTTGCTTAGTTTGATGAAACATAGTGGTTTAGTTCCTGACCAGTGGACTTACAGTGTCATCATTGACAGTCTCTGTAAGTTGGGGAGATTAAAAGAAGCTCAAGCCCTCTTCGATTCTCTCAAGGACAAAGGCATCAAATCTAATGAAGTGATATTTACGGCTTTACTTGATGGTTACTGCAAGATTGGGAAAATCGATGATGCTCGCACTTTATTTAATAGAATGCTTGCTGAGGGTTGTTTCCCAAACTCATATACTTACTGTGCCTTCCTAGGTGGGCTGTGCAATGAGGGAAAAACAAAGGAAGCATTATCAGTGGTGGAACAGATGCTAAGTAGTGGTGTGAAGCCGACAGTCCACACTTGTAACATTCTAATTAACCATGTGCTGAAAGAAGGGGATTTCAACCATGCTCAGAGGTTTTTGAATGAATTTGTTTCTCTTGGTTACCGACCTGATGCAGTCATGTACGGTACATTTGTTCATGCATATTGCAGCATAGATAATATAAAAGAAGCAGAGAAGGTAATGACTAAGATGGATGAAGAAGGAATTTCTGCAGATTCATTGACATACGCATTTTTAATCGAGGCATATCTTCGTTTGGGATTAATAGATTCTGCATTTGATGTCTTTAAGCGCATGTTTGATGCTCATTGCAACCCTTGTCATCGCATCTATTACTTGCTCATCAAACACCTGTCAAATGAAAAGTTGCTGCAGACCGACGATAATGTAGTAGGACTTCATATGGTTGCAAGTGTCCCATCAGCTGATATGTGGAAGACAGTGGATTTTGAAATTGCTTTAGAGCTCCTTGAGAAGATGAATGAACATGGTTGTGCACCGAATGTGAAAACTTATGGTGAGCTTATAGTAGCACTTTGCAAAGTTAGGAACTTGGAAGTAGCCCGTAAGTTGTATGCTCATATGAGAGATACCAGCATTTCTCCTAGTGAGGATATTTGTAACTCTCTTCTTAAATGTTGCTGCGAGTTGAAGGTGTATGGAGAAGCAGCTATTCTGGTGGACGCTATGATTCAGCTTGGTTATTTACCAGCATTGGAGTCGTGCAAATTGCTTGTATGTGGACTTTTTGAAGAAGAGAATAGTGAGAAGGCAAATTCTGTTTTCTGCAGAATTCTTTGTTGCGCGTATAACTACGATGAAGTAGCCTGGAAAGTTCTTCTTGAAGGTTTACTTACGAGGGGTCATATCAATAGATGCTCTGAGATGGTAACCGTCATCGAAAATATGGGTTACAATCTTCATCCTGAGACCTATTCAATGTTAATTGAGGGATTTGATGGAATATAA

Protein Analysis

662

Amino Acids

74.28

Weight (kDa)

5.81

Isoelectric Point (pI)

28.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 2 - 39 7.2e-08 PPR repeat family
TPR_24 PF23276 5 - 117 6.5e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 5 - 142 6.4e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 21 - 72 2.6e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 22 - 55 2.8e-11 PPR repeat
PPR_2 PF13041 26 - 75 1.4e-18 PPR repeat family
PPR PF01535 29 - 59 5.7e-07 PPR repeat
PPR_1 PF12854 58 - 90 8.4e-08 PPR repeat
PPR_2 PF13041 65 - 110 8.6e-15 PPR repeat family
PPR PF01535 65 - 94 3e-08 PPR repeat
PPR_3 PF13812 69 - 106 2.7e-06 Pentatricopeptide repeat domain
PPR_long PF17177 83 - 244 3.9e-14 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 92 - 124 4.3e-08 PPR repeat
PPR_2 PF13041 96 - 137 1.9e-09 PPR repeat family
TPR_24 PF23276 116 - 195 4.5e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 119 - 175 8e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 127 - 160 3.1e-12 PPR repeat
PPR_2 PF13041 131 - 180 1e-17 PPR repeat family
PPR PF01535 134 - 164 1.7e-07 PPR repeat
PPR_1 PF12854 164 - 194 7.6e-10 PPR repeat
PPR_3 PF13812 169 - 211 2.5e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 170 - 215 1.9e-13 PPR repeat family
PPR_1 PF12854 199 - 230 5.1e-12 PPR repeat
PPR_2 PF13041 201 - 250 4e-17 PPR repeat family
PPR PF01535 204 - 234 1.2e-06 PPR repeat
PPR_1 PF12854 233 - 263 5.4e-10 PPR repeat
PPR PF01535 240 - 269 2.4e-06 PPR repeat
PPR_long PF17177 254 - 369 2.3e-09 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 257 - 364 2.5e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 260 - 316 1e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 267 - 299 6.8e-09 PPR repeat
PPR_2 PF13041 272 - 320 3.2e-15 PPR repeat family
PPR PF01535 274 - 304 2.5e-07 PPR repeat
PPR_1 PF12854 303 - 334 4e-08 PPR repeat
PPR_3 PF13812 306 - 352 4.6e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 314 - 351 4.3e-09 PPR repeat family
PPR_3 PF13812 366 - 423 1.3e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 373 - 404 6.5e-06 PPR repeat
PPR_2 PF13041 376 - 423 1.3e-08 PPR repeat family
PPR_long PF17177 486 - 556 8.8e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 489 - 521 1e-05 PPR repeat family
PPR_3 PF13812 496 - 554 9.6e-08 Pentatricopeptide repeat domain
PPR_long PF17177 528 - 655 4.7e-06 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
Acc36I ACCTGC 1 cut(s) 638
AccB7I CCANNNNNTGG 1 cut(s) 562
AccBSI CCGCTC 1 cut(s) 443
AccII CGCG 1 cut(s) 1826
AccIII TCCGGA 1 cut(s) 391
AciI CCGC 1 cut(s) 443
AclWI GGATC 1 cut(s) 49
AcsI RAATTY 4 cut(s) 1103, 1224, 1795, 1812
AcuI CTGAAG 1 cut(s) 326
AfaI GTAC 4 cut(s) 152, 407, 1142, 1147
AfiI CCNNNNNNNGG 4 cut(s) 64, 65, 373, 562
AgsI TTSAA 8 cut(s) 587, 1078, 1099, 1472, 1672, 1772, 1865, 1959
AhdI GACNNNNNGTC 1 cut(s) 370
AjnI CCWGG 2 cut(s) 555, 1847
AluBI AGCT 8 cut(s) 340, 595, 770, 1445, 1486, 1543, 1691, 1717
AluI AGCT 8 cut(s) 340, 595, 770, 1445, 1486, 1543, 1691, 1717
Alw21I GWGCWC 4 cut(s) 312, 555, 1488, 1519
Alw26I GTCTC 2 cut(s) 746, 1943
Alw44I GTGCAC 2 cut(s) 551, 1515
AlwI GGATC 1 cut(s) 49
AlwNI CAGNNNCTG 1 cut(s) 744
Aor13HI TCCGGA 1 cut(s) 391
ApaLI GTGCAC 2 cut(s) 551, 1515
ApeKI GCWGC 5 cut(s) 452, 1167, 1390, 1662, 1688
ApoI RAATTY 4 cut(s) 1103, 1224, 1795, 1812
AseI ATTAAT 1 cut(s) 1283
Asp700I GAANNNNTTC 1 cut(s) 522
AspA2I CCTAGG 1 cut(s) 943
AspLEI GCGC 4 cut(s) 190, 568, 1314, 1826
AsuHPI GGTGA 3 cut(s) 64, 591, 1550
AsuII TTCGAA 1 cut(s) 240
AvrII CCTAGG 1 cut(s) 943
BaeGI GKGCMC 2 cut(s) 555, 1519
BaeI ACNNNNGTAYC 2 cut(s) 1137, 1170
BanII GRGCYC 2 cut(s) 114, 1488
BarI GAAGNNNNNNTAC 2 cut(s) 1401, 1433
BbsI GAAGAC 1 cut(s) 1463
Bbv12I GWGCWC 4 cut(s) 312, 555, 1488, 1519
BbvCI CCTCAGC 1 cut(s) 903
BbvI GCAGC 5 cut(s) 439, 1179, 1377, 1649, 1700
BccI CCATC 7 cut(s) 416, 469, 836, 1204, 1447, 1900, 1973
BceAI ACGGC 1 cut(s) 846
BcgI CGANNNNNNTGC 2 cut(s) 1722, 1756
BciT130I CCWGG 2 cut(s) 557, 1849
BclI TGATCA 1 cut(s) 627
BcoDI GTCTC 2 cut(s) 746, 1943
BfaI CTAG 6 cut(s) 288, 318, 351, 362, 944, 1626
BfmI CTRYAG 3 cut(s) 1230, 1391, 1807
BfoI RGCGCY 1 cut(s) 569
BfuAI ACCTGC 1 cut(s) 638
BisI GCNGC 5 cut(s) 453, 1168, 1391, 1663, 1689
BlnI CCTAGG 1 cut(s) 943
BlsI GCNGC 5 cut(s) 454, 1169, 1392, 1664, 1690
Bme1390I CCNGG 2 cut(s) 557, 1849
BmeRI GACNNNNNGTC 1 cut(s) 370
BmiI GGNNCC 1 cut(s) 390
BmrFI CCNGG 2 cut(s) 557, 1849
BmsI GCATC 9 cut(s) 175, 570, 813, 862, 990, 1120, 1312, 1356, 1886
BpiI GAAGAC 1 cut(s) 1463
BplI GAGNNNNNCTC 2 cut(s) 137, 169
Bpu10I CCTNAGC 1 cut(s) 903
Bpu14I TTCGAA 1 cut(s) 240
BpuEI CTTGAG 3 cut(s) 756, 776, 1511
Bsa29I ATCGAT 1 cut(s) 867
BsaAI YACGTR 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 1943
BsaJI CCNNGG 1 cut(s) 943
BsaWI WCCGGW 1 cut(s) 391
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 65, 373, 562
Bse1I ACTGG 1 cut(s) 712
Bse3DI GCAATG 3 cut(s) 567, 964, 1327
BseAI TCCGGA 1 cut(s) 391
BseBI CCWGG 2 cut(s) 557, 1849
BseCI ATCGAT 1 cut(s) 867
BseDI CCNNGG 1 cut(s) 943
BseGI GGATG 6 cut(s) 28, 175, 400, 585, 1219, 1942
BseLI CCNNNNNNNGG 4 cut(s) 64, 65, 373, 562
BseMI GCAATG 3 cut(s) 567, 964, 1327
BseMII CTCAG 4 cut(s) 894, 1100, 1893, 1938
BseNI ACTGG 1 cut(s) 712
BseRI GAGGAG 1 cut(s) 247
BseSI GKGCMC 2 cut(s) 555, 1519
BseXI GCAGC 5 cut(s) 439, 1179, 1377, 1649, 1700
Bsh1236I CGCG 1 cut(s) 1826
BshVI ATCGAT 1 cut(s) 867
BsiHKAI GWGCWC 4 cut(s) 312, 555, 1488, 1519
BsiSI CCGG 1 cut(s) 392
BslFI GGGAC 1 cut(s) 1421
BslI CCNNNNNNNGG 4 cut(s) 64, 65, 373, 562
BsmAI GTCTC 2 cut(s) 746, 1943
BsmFI GGGAC 1 cut(s) 1421
BsmI GAATGC 1 cut(s) 900
Bso31I GGTCTC 1 cut(s) 1943
Bsp119I TTCGAA 1 cut(s) 240
Bsp1286I GDGCHC 5 cut(s) 114, 312, 555, 1488, 1519
Bsp13I TCCGGA 1 cut(s) 391
Bsp143I GATC 2 cut(s) 54, 627
BspACI CCGC 1 cut(s) 443
BspCNI CTCAG 4 cut(s) 895, 1099, 1894, 1939
BspDI ATCGAT 1 cut(s) 867
BspEI TCCGGA 1 cut(s) 391
BspFNI CGCG 1 cut(s) 1826
BspLI GGNNCC 1 cut(s) 390
BspMAI CTGCAG 3 cut(s) 1234, 1395, 1811
BspMI ACCTGC 1 cut(s) 638
BspPI GGATC 1 cut(s) 49
BspT104I TTCGAA 1 cut(s) 240
BspTNI GGTCTC 1 cut(s) 1943
BsrBI CCGCTC 1 cut(s) 443
BsrDI GCAATG 3 cut(s) 567, 964, 1327
BsrI ACTGG 1 cut(s) 712
BssECI CCNNGG 1 cut(s) 943
BssMI GATC 2 cut(s) 54, 627
BssT1I CCWWGG 1 cut(s) 943
Bst2UI CCWGG 2 cut(s) 557, 1849
Bst4CI ACNGT 7 cut(s) 725, 740, 935, 1027, 1145, 1462, 1915
Bst6I CTCTTC 3 cut(s) 785, 1653, 1770
BstBAI YACGTR 1 cut(s) 405
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 4 cut(s) 124, 505, 877, 900
BstDEI CTNAG 7 cut(s) 677, 903, 1004, 1086, 1206, 1902, 1947
BstEII GGTNACC 2 cut(s) 1118, 1909
BstF5I GGATG 6 cut(s) 28, 175, 400, 585, 1219, 1942
BstFNI CGCG 1 cut(s) 1826
BstH2I RGCGCY 1 cut(s) 569
BstHHI GCGC 4 cut(s) 190, 568, 1314, 1826
BstKTI GATC 2 cut(s) 57, 630
BstMAI GTCTC 2 cut(s) 746, 1943
BstMBI GATC 2 cut(s) 54, 627
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstNI CCWGG 2 cut(s) 557, 1849
BstNSI RCATGY 1 cut(s) 1318
BstPI GGTNACC 2 cut(s) 1118, 1909
BstSCI CCNGG 2 cut(s) 555, 1847
BstSFI CTRYAG 3 cut(s) 1230, 1391, 1807
BstSLI GKGCMC 2 cut(s) 555, 1519
BstUI CGCG 1 cut(s) 1826
BstV1I GCAGC 5 cut(s) 439, 1179, 1377, 1649, 1700
BstV2I GAAGAC 1 cut(s) 1463
Bsu15I ATCGAT 1 cut(s) 867
BsuTUI ATCGAT 1 cut(s) 867
BtgZI GCGATG 1 cut(s) 1328
BtsCI GGATG 6 cut(s) 28, 175, 400, 585, 1219, 1942
BtsI GCAGTG 1 cut(s) 644
BtsIMutI CAGTG 5 cut(s) 644, 719, 730, 993, 1467
BveI ACCTGC 1 cut(s) 638
Cac8I GCNNGC 4 cut(s) 124, 505, 877, 900
CaiI CAGNNNCTG 1 cut(s) 744
CfoI GCGC 4 cut(s) 190, 568, 1314, 1826
ClaI ATCGAT 1 cut(s) 867
CseI GACGC 1 cut(s) 1712
Csp6I GTAC 4 cut(s) 151, 406, 1141, 1146
CspCI CAANNNNNGTGG 2 cut(s) 63, 98
CviAII CATG 9 cut(s) 212, 527, 653, 1055, 1082, 1138, 1157, 1315, 1508
CviQI GTAC 4 cut(s) 151, 406, 1141, 1146
DdeI CTNAG 7 cut(s) 677, 903, 1004, 1086, 1206, 1902, 1947
DpnI GATC 2 cut(s) 56, 629
DpnII GATC 2 cut(s) 54, 627
DriI GACNNNNNGTC 1 cut(s) 370
Eam1104I CTCTTC 3 cut(s) 785, 1653, 1770
Eam1105I GACNNNNNGTC 1 cut(s) 370
EarI CTCTTC 3 cut(s) 785, 1653, 1770
Ecl136II GAGCTC 1 cut(s) 1486
Eco130I CCWWGG 1 cut(s) 943
Eco24I GRGCYC 2 cut(s) 114, 1488
Eco31I GGTCTC 1 cut(s) 1943
Eco53kI GAGCTC 1 cut(s) 1486
Eco57I CTGAAG 1 cut(s) 326
Eco91I GGTNACC 2 cut(s) 1118, 1909
EcoICRI GAGCTC 1 cut(s) 1486
EcoO65I GGTNACC 2 cut(s) 1118, 1909
EcoRI GAATTC 1 cut(s) 1812
EcoRII CCWGG 2 cut(s) 555, 1847
EcoT14I CCWWGG 1 cut(s) 943
EcoT22I ATGCAT 3 cut(s) 217, 415, 1162
EcoT38I GRGCYC 2 cut(s) 114, 1488
ErhI CCWWGG 1 cut(s) 943
FaeI CATG 9 cut(s) 215, 530, 656, 1058, 1085, 1141, 1160, 1318, 1511
FalI AAGNNNNNCTT 4 cut(s) 1845, 1877, 1857, 1889
FaqI GGGAC 1 cut(s) 1421
FatI CATG 9 cut(s) 211, 526, 652, 1054, 1081, 1137, 1156, 1314, 1507
FauNDI CATATG 2 cut(s) 1422, 1602
FbaI TGATCA 1 cut(s) 627
Fnu4HI GCNGC 5 cut(s) 453, 1168, 1391, 1663, 1689
FokI GGATG 6 cut(s) 35, 182, 407, 592, 1226, 1929
FriOI GRGCYC 2 cut(s) 114, 1488
Fsp4HI GCNGC 5 cut(s) 453, 1168, 1391, 1663, 1689
FspBI CTAG 6 cut(s) 288, 318, 351, 362, 944, 1626
GlaI GCGC 4 cut(s) 189, 567, 1313, 1825
GluI GCNGC 5 cut(s) 453, 1168, 1391, 1663, 1689
HaeII RGCGCY 1 cut(s) 569
HapII CCGG 1 cut(s) 392
HgaI GACGC 1 cut(s) 1712
HhaI GCGC 4 cut(s) 190, 568, 1314, 1826
Hin1II CATG 9 cut(s) 215, 530, 656, 1058, 1085, 1141, 1160, 1318, 1511
Hin6I GCGC 4 cut(s) 188, 566, 1312, 1824
HinP1I GCGC 4 cut(s) 188, 566, 1312, 1824
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 9 cut(s) 237, 463, 479, 523, 785, 1235, 1289, 1711, 1739
HpaII CCGG 1 cut(s) 392
HphI GGTGA 3 cut(s) 64, 591, 1550
Hpy166II GTNNAC 9 cut(s) 179, 513, 553, 717, 1030, 1517, 1702, 1763, 1871
Hpy188I TCNGA 2 cut(s) 1089, 1903
Hpy188III TCNNGA 6 cut(s) 362, 392, 572, 707, 1862, 1946
Hpy8I GTNNAC 9 cut(s) 179, 513, 553, 717, 1030, 1517, 1702, 1763, 1871
Hpy99I CGWCG 2 cut(s) 196, 1403
HpyCH4III ACNGT 7 cut(s) 725, 740, 935, 1027, 1145, 1462, 1915
HpyCH4IV ACGT 3 cut(s) 194, 404, 509
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 7 cut(s) 677, 903, 1004, 1086, 1206, 1902, 1947
HpySE526I ACGT 3 cut(s) 194, 404, 509
Hsp92II CATG 9 cut(s) 215, 530, 656, 1058, 1085, 1141, 1160, 1318, 1511
HspAI GCGC 4 cut(s) 188, 566, 1312, 1824
Kpn2I TCCGGA 1 cut(s) 391
Ksp22I TGATCA 1 cut(s) 627
Kzo9I GATC 2 cut(s) 54, 627
LmnI GCTCC 2 cut(s) 440, 1491
Lsp1109I GCAGC 5 cut(s) 439, 1179, 1377, 1649, 1700
LweI GCATC 9 cut(s) 175, 570, 813, 862, 990, 1120, 1312, 1356, 1886
MaeI CTAG 6 cut(s) 288, 318, 351, 362, 944, 1626
MaeII ACGT 3 cut(s) 194, 404, 509
MaeIII GTNAC 9 cut(s) 131, 326, 400, 845, 1037, 1118, 1640, 1909, 1931
MalI GATC 2 cut(s) 56, 629
MbiI CCGCTC 1 cut(s) 443
MboI GATC 2 cut(s) 54, 627
MhlI GDGCHC 5 cut(s) 114, 312, 555, 1488, 1519
MlyI GAGTC 1 cut(s) 1748
Mph1103I ATGCAT 3 cut(s) 217, 415, 1162
MroI TCCGGA 1 cut(s) 391
MroXI GAANNNNTTC 1 cut(s) 522
MslI CAYNNNNRTG 1 cut(s) 1512
MspA1I CMGCKG 1 cut(s) 1445
MspI CCGG 1 cut(s) 392
MspR9I CCNGG 2 cut(s) 557, 1849
Mva1269I GAATGC 1 cut(s) 900
MvaI CCWGG 2 cut(s) 557, 1849
MvnI CGCG 1 cut(s) 1826
MwoI GCNNNNNNNGC 1 cut(s) 449
NdeI CATATG 2 cut(s) 1422, 1602
NdeII GATC 2 cut(s) 54, 627
NlaIII CATG 9 cut(s) 215, 530, 656, 1058, 1085, 1141, 1160, 1318, 1511
NlaIV GGNNCC 1 cut(s) 390
NmuCI GTSAC 2 cut(s) 326, 400
NsiI ATGCAT 3 cut(s) 217, 415, 1162
NspI RCATGY 1 cut(s) 1318
NspV TTCGAA 1 cut(s) 240
PctI GAATGC 1 cut(s) 900
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 8 cut(s) 237, 463, 479, 523, 785, 1235, 1289, 1711
PflMI CCANNNNNTGG 1 cut(s) 562
PkrI GCNGC 5 cut(s) 454, 1169, 1392, 1664, 1690
PleI GAGTC 1 cut(s) 1747
PpsI GAGTC 1 cut(s) 1747
Ppu21I YACGTR 1 cut(s) 405
PshBI ATTAAT 1 cut(s) 1283
PsiI TTATAA 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 1488
Psp6I CCWGG 2 cut(s) 555, 1847
PspEI GGTNACC 2 cut(s) 1118, 1909
PspGI CCWGG 2 cut(s) 555, 1847
PspN4I GGNNCC 1 cut(s) 390
PstI CTGCAG 3 cut(s) 1234, 1395, 1811
PstNI CAGNNNCTG 1 cut(s) 744
PvuII CAGCTG 1 cut(s) 1445
RsaI GTAC 4 cut(s) 152, 407, 1142, 1147
RsaNI GTAC 4 cut(s) 151, 406, 1141, 1146
RseI CAYNNNNRTG 1 cut(s) 1512
SacI GAGCTC 1 cut(s) 1488
SatI GCNGC 5 cut(s) 453, 1168, 1391, 1663, 1689
Sau3AI GATC 2 cut(s) 54, 627
SchI GAGTC 1 cut(s) 1748
ScrFI CCNGG 2 cut(s) 557, 1849
SduI GDGCHC 5 cut(s) 114, 312, 555, 1488, 1519
SfaNI GCATC 9 cut(s) 175, 570, 813, 862, 990, 1120, 1312, 1356, 1886
SfcI CTRYAG 3 cut(s) 1230, 1391, 1807
SfuI TTCGAA 1 cut(s) 240
SmiMI CAYNNNNRTG 1 cut(s) 1512
SmlI CTYRAG 3 cut(s) 771, 791, 1490
SmoI CTYRAG 3 cut(s) 771, 791, 1490
SsiI CCGC 1 cut(s) 443
SspMI CTAG 6 cut(s) 288, 318, 351, 362, 944, 1626
SstI GAGCTC 1 cut(s) 1488
StyD4I CCNGG 2 cut(s) 555, 1847
StyI CCWWGG 1 cut(s) 943
TaaI ACNGT 7 cut(s) 725, 740, 935, 1027, 1145, 1462, 1915
TaiI ACGT 3 cut(s) 197, 407, 512
TaqI TCGA 5 cut(s) 240, 783, 867, 1260, 1920
TaqII GACCGA 1 cut(s) 1412
TatI WGTACW 1 cut(s) 150
TfiI GAWTC 8 cut(s) 237, 463, 479, 523, 785, 1235, 1289, 1711
TscAI CASTG 5 cut(s) 644, 719, 730, 993, 1467
TseFI GTSAC 2 cut(s) 326, 400
TseI GCWGC 5 cut(s) 452, 1167, 1390, 1662, 1688
Tsp45I GTSAC 2 cut(s) 326, 400
TspGWI ACGGA 1 cut(s) 604
TspRI CASTG 5 cut(s) 644, 719, 730, 993, 1467
Van91I CCANNNNNTGG 1 cut(s) 562
VneI GTGCAC 2 cut(s) 551, 1515
VspI ATTAAT 1 cut(s) 1283
XapI RAATTY 4 cut(s) 1103, 1224, 1795, 1812
XbaI TCTAGA 1 cut(s) 361
XceI RCATGY 1 cut(s) 1318
XmaJI CCTAGG 1 cut(s) 943
XmnI GAANNNNTTC 1 cut(s) 522
XspI CTAG 6 cut(s) 288, 318, 351, 362, 944, 1626
Zsp2I ATGCAT 3 cut(s) 217, 415, 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.