MD11G1188000.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001743
Physical Location & Seq
Forward (+)
26445798 .. 26446898
1101 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1188000.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGCCTACCGAGGATTGTTTGCCAAACTCATATGCCATCACTGCCTTGATACATGGGCTATTCAAAGAGAGAAAATTGATGGAAGCATCCTCAGTCATGGAAACAATGTTAAATATAGGTGTGAAGCCTACAGTCCCTCATTACAATATTTTAATTAATCGTGTTCTGAAAGAAGGTGAATTTGATCATGCTCATGAGCTTCTCAATAAAATGGTTTCTGTCGGTAACCAACTTACTGTAATTACTTACAACACATTTATTCAGGCATATTGCGTGCTTGGGAATATAAAGGAAGCAGAGAAGGTGATGATTAAGATGAATGTAGAAGGAATTTCTGCAGATTCATTGACTTACACATTTTTAATTGATGCATATGTGCGTATGGGATTAATTGATTGTGCTTTTGATGTTCTTAACCGCATGTTTGATTCTGGTTGTGAGCCTTGTCGTCGTACCTATTCCTTTCTAATCAAATATCTCTCAAATGAAAAGTTGGTCATGACAAACAGTAAAGCAGTGGGTCTCGATATCATGTTAAATGTCTCTGGCAATATTACTCATATATGGAAGACCATGGATTTTGAAATTGCTTTGGAGCTATTTGAGAAGATGGTTGGACATGGTTGTGCTCCCAATATGAACACTTATGGCGAGCTTATAGTAAGATTTTGCAAAGACAGAAACTTGCAAGTAGCTCAGAGGTTATATAATCATATGAGAGATATGGGTATTTCTCCTAACGAAGGTATTTATAATTCTCTTGTTAATTATTGCTGTCAGATGCAAGTATACGGAGAGGCAGCAATGCTAGTGCATACTATGATTGAGCATGGTTATTTACTAGCATTGGAGTCATGCAAGTTGCTTGTATGTGGACTTTTTGATGAAGAGGACGACGAGAAGGCTAAGGCAGTTTTTTGTAGTATGCTTCGCTGTGGGTATAACTATGATGAAGTTGCTTGGAAACTTCTCCTTGATGGTTTGGTTAAGAGGGGCCATTTAAATAGAGGATCAGAGCTGCTAACAATCATGGAGAAGATGGGTTGCCAGCTTCATCCGGAGACAAATAGAATGTTGACCAAGGGACTTAATGGAACATGA

Protein Analysis

367

Amino Acids

41.51

Weight (kDa)

5.8

Isoelectric Point (pI)

29.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 7 - 53 2.6e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 8 - 53 6.2e-07 PPR repeat family
PPR_long PF17177 26 - 163 4.3e-10 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 43 - 91 4.8e-11 PPR repeat family
PPR_3 PF13812 67 - 126 5.5e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 78 - 106 4.5e-07 PPR repeat
PPR_2 PF13041 79 - 126 7.6e-13 PPR repeat family
PPR PF01535 81 - 111 1.2e-06 PPR repeat
PPR_3 PF13812 105 - 160 6.7e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 110 - 141 2.2e-06 PPR repeat
PPR_2 PF13041 114 - 160 1.6e-09 PPR repeat family
PPR PF01535 117 - 146 9.1e-06 PPR repeat
PPR_long PF17177 184 - 357 9.7e-10 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 200 - 259 4.9e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 215 - 260 3.3e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 753
AccI GTMKAC 1 cut(s) 789
AccIII TCCGGA 1 cut(s) 1057
AciI CCGC 1 cut(s) 418
AclWI GGATC 1 cut(s) 1018
AcsI RAATTY 2 cut(s) 179, 330
AfaI GTAC 1 cut(s) 454
AfiI CCNNNNNNNGG 1 cut(s) 743
AgsI TTSAA 2 cut(s) 64, 584
AluBI AGCT 6 cut(s) 199, 598, 655, 695, 1018, 1051
AluI AGCT 6 cut(s) 199, 598, 655, 695, 1018, 1051
Alw21I GWGCWC 1 cut(s) 631
Alw26I GTCTC 3 cut(s) 527, 547, 1055
AlwI GGATC 1 cut(s) 1018
Aor13HI TCCGGA 1 cut(s) 1057
AoxI GGCC 1 cut(s) 994
ApeKI GCWGC 2 cut(s) 800, 1018
ApoI RAATTY 2 cut(s) 179, 330
AseI ATTAAT 2 cut(s) 156, 389
AspS9I GGNCC 1 cut(s) 994
AsuHPI GGTGA 2 cut(s) 188, 316
BbsI GAAGAC 1 cut(s) 575
Bbv12I GWGCWC 1 cut(s) 631
BbvI GCAGC 2 cut(s) 812, 1005
BccI CCATC 5 cut(s) 44, 73, 604, 971, 1033
BclI TGATCA 1 cut(s) 184
BcoDI GTCTC 3 cut(s) 527, 547, 1055
BfaI CTAG 2 cut(s) 809, 842
BfmI CTRYAG 2 cut(s) 129, 336
BisI GCNGC 2 cut(s) 801, 1019
BlsI GCNGC 2 cut(s) 802, 1020
BmgT120I GGNCC 1 cut(s) 994
BmiI GGNNCC 1 cut(s) 995
BmsI GCATC 3 cut(s) 95, 358, 771
BpiI GAAGAC 1 cut(s) 575
Bpu10I CCTNAGC 1 cut(s) 906
BsaI GGTCTC 1 cut(s) 527
BsaJI CCNNGG 3 cut(s) 9, 573, 1080
BsaWI WCCGGW 1 cut(s) 1057
Bsc4I CCNNNNNNNGG 1 cut(s) 743
Bse3DI GCAATG 1 cut(s) 810
BseAI TCCGGA 1 cut(s) 1057
BseDI CCNNGG 3 cut(s) 9, 573, 1080
BseGI GGATG 2 cut(s) 86, 1054
BseLI CCNNNNNNNGG 1 cut(s) 743
BseMI GCAATG 1 cut(s) 810
BseMII CTCAG 2 cut(s) 105, 710
BseXI GCAGC 2 cut(s) 812, 1005
BshFI GGCC 1 cut(s) 996
BsiHKAI GWGCWC 1 cut(s) 631
BsiSI CCGG 1 cut(s) 1058
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 1 cut(s) 743
BsmAI GTCTC 3 cut(s) 527, 547, 1055
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 1 cut(s) 996
Bso31I GGTCTC 1 cut(s) 527
Bsp1286I GDGCHC 1 cut(s) 631
Bsp13I TCCGGA 1 cut(s) 1057
Bsp143I GATC 2 cut(s) 184, 1010
Bsp19I CCATGG 1 cut(s) 573
BspACI CCGC 1 cut(s) 418
BspANI GGCC 1 cut(s) 996
BspCNI CTCAG 2 cut(s) 104, 709
BspEI TCCGGA 1 cut(s) 1057
BspHI TCATGA 2 cut(s) 193, 498
BspLI GGNNCC 1 cut(s) 995
BspMAI CTGCAG 1 cut(s) 340
BspPI GGATC 1 cut(s) 1018
BspTNI GGTCTC 1 cut(s) 527
BsrDI GCAATG 1 cut(s) 810
BssECI CCNNGG 3 cut(s) 9, 573, 1080
BssMI GATC 2 cut(s) 184, 1010
BssNAI GTATAC 1 cut(s) 790
BssT1I CCWWGG 2 cut(s) 573, 1080
Bst1107I GTATAC 1 cut(s) 790
Bst4CI ACNGT 3 cut(s) 133, 238, 509
Bst6I CTCTTC 1 cut(s) 882
BstC8I GCNNGC 3 cut(s) 275, 653, 1049
BstDEI CTNAG 3 cut(s) 91, 696, 906
BstDSI CCRYGG 1 cut(s) 573
BstEII GGTNACC 1 cut(s) 224
BstENI CCTNNNNNAGG 1 cut(s) 741
BstF5I GGATG 2 cut(s) 86, 1054
BstKTI GATC 2 cut(s) 187, 1013
BstMAI GTCTC 3 cut(s) 527, 547, 1055
BstMBI GATC 2 cut(s) 184, 1010
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 424
BstPI GGTNACC 1 cut(s) 224
BstSFI CTRYAG 2 cut(s) 129, 336
BstV1I GCAGC 2 cut(s) 812, 1005
BstV2I GAAGAC 1 cut(s) 575
BstZ17I GTATAC 1 cut(s) 790
BsuRI GGCC 1 cut(s) 996
BtgI CCRYGG 1 cut(s) 573
BtsCI GGATG 2 cut(s) 86, 1054
BtsI GCAGTG 2 cut(s) 39, 522
BtsIMutI CAGTG 2 cut(s) 39, 522
Cac8I GCNNGC 3 cut(s) 275, 653, 1049
CciI TCATGA 2 cut(s) 193, 498
Cfr13I GGNCC 1 cut(s) 994
Csp6I GTAC 1 cut(s) 453
CviQI GTAC 1 cut(s) 453
DdeI CTNAG 3 cut(s) 91, 696, 906
DpnI GATC 2 cut(s) 186, 1012
DpnII GATC 2 cut(s) 184, 1010
DraI TTTAAA 1 cut(s) 1002
Eam1104I CTCTTC 1 cut(s) 882
EarI CTCTTC 1 cut(s) 882
Eco130I CCWWGG 2 cut(s) 573, 1080
Eco31I GGTCTC 1 cut(s) 527
Eco32I GATATC 1 cut(s) 529
Eco91I GGTNACC 1 cut(s) 224
EcoNI CCTNNNNNAGG 1 cut(s) 741
EcoO65I GGTNACC 1 cut(s) 224
EcoRV GATATC 1 cut(s) 529
EcoT14I CCWWGG 2 cut(s) 573, 1080
EcoT22I ATGCAT 1 cut(s) 373
ErhI CCWWGG 2 cut(s) 573, 1080
FaqI GGGAC 1 cut(s) 119
FauNDI CATATG 3 cut(s) 31, 373, 714
FbaI TGATCA 1 cut(s) 184
FblI GTMKAC 1 cut(s) 789
Fnu4HI GCNGC 2 cut(s) 801, 1019
FokI GGATG 2 cut(s) 73, 1041
Fsp4HI GCNGC 2 cut(s) 801, 1019
FspBI CTAG 2 cut(s) 809, 842
GluI GCNGC 2 cut(s) 801, 1019
HaeIII GGCC 1 cut(s) 996
HapII CCGG 1 cut(s) 1058
HincII GTYRAC 1 cut(s) 1077
HindII GTYRAC 1 cut(s) 1077
HinfI GANTC 3 cut(s) 341, 428, 851
HpaII CCGG 1 cut(s) 1058
HphI GGTGA 2 cut(s) 188, 316
Hpy166II GTNNAC 3 cut(s) 790, 875, 1077
Hpy188I TCNGA 4 cut(s) 168, 699, 780, 1015
Hpy188III TCNNGA 4 cut(s) 194, 499, 524, 1058
Hpy8I GTNNAC 3 cut(s) 790, 875, 1077
Hpy99I CGWCG 2 cut(s) 453, 899
HpyAV CCTTC 5 cut(s) 167, 295, 320, 737, 895
HpyCH4III ACNGT 3 cut(s) 133, 238, 509
HpyCH4V TGCA 7 cut(s) 338, 371, 672, 688, 784, 814, 858
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 3 cut(s) 91, 696, 906
Kpn2I TCCGGA 1 cut(s) 1057
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 2 cut(s) 184, 1010
LmnI GCTCC 2 cut(s) 595, 634
LpnPI CCDG 5 cut(s) 248, 417, 531, 1061, 1071
Lsp1109I GCAGC 2 cut(s) 812, 1005
LweI GCATC 3 cut(s) 95, 358, 771
MaeI CTAG 2 cut(s) 809, 842
MaeIII GTNAC 1 cut(s) 224
MalI GATC 2 cut(s) 186, 1012
MboI GATC 2 cut(s) 184, 1010
MboII GAAGA 4 cut(s) 580, 619, 899, 1048
MhlI GDGCHC 1 cut(s) 631
MlyI GAGTC 1 cut(s) 860
MmeI TCCRAC 1 cut(s) 595
MnlI CCTC 8 cut(s) 4, 100, 147, 693, 790, 883, 984, 1001
Mph1103I ATGCAT 1 cut(s) 373
MroI TCCGGA 1 cut(s) 1057
MslI CAYNNNNRTG 2 cut(s) 192, 624
MspI CCGG 1 cut(s) 1058
MwoI GCNNNNNNNGC 1 cut(s) 41
NcoI CCATGG 1 cut(s) 573
NdeI CATATG 3 cut(s) 31, 373, 714
NdeII GATC 2 cut(s) 184, 1010
NlaIV GGNNCC 1 cut(s) 995
NsiI ATGCAT 1 cut(s) 373
NspI RCATGY 1 cut(s) 424
PacI TTAATTAA 1 cut(s) 156
PagI TCATGA 2 cut(s) 193, 498
PfeI GAWTC 2 cut(s) 341, 428
PkrI GCNGC 2 cut(s) 802, 1020
PleI GAGTC 1 cut(s) 859
PpsI GAGTC 1 cut(s) 859
PshBI ATTAAT 2 cut(s) 156, 389
PsiI TTATAA 1 cut(s) 753
PspEI GGTNACC 1 cut(s) 224
PspN4I GGNNCC 1 cut(s) 995
PspPI GGNCC 1 cut(s) 994
PstI CTGCAG 1 cut(s) 340
RsaI GTAC 1 cut(s) 454
RsaNI GTAC 1 cut(s) 453
RseI CAYNNNNRTG 2 cut(s) 192, 624
SatI GCNGC 2 cut(s) 801, 1019
Sau3AI GATC 2 cut(s) 184, 1010
Sau96I GGNCC 1 cut(s) 994
SchI GAGTC 1 cut(s) 860
SduI GDGCHC 1 cut(s) 631
SfaNI GCATC 3 cut(s) 95, 358, 771
SfcI CTRYAG 2 cut(s) 129, 336
SmiI ATTTAAAT 1 cut(s) 1002
SmiMI CAYNNNNRTG 2 cut(s) 192, 624
SsiI CCGC 1 cut(s) 418
SspI AATATT 2 cut(s) 148, 553
SspMI CTAG 2 cut(s) 809, 842
StyI CCWWGG 2 cut(s) 573, 1080
SwaI ATTTAAAT 1 cut(s) 1002
TaaI ACNGT 3 cut(s) 133, 238, 509
TaqI TCGA 1 cut(s) 525
TfiI GAWTC 2 cut(s) 341, 428
TscAI CASTG 2 cut(s) 46, 522
TseI GCWGC 2 cut(s) 800, 1018
TspDTI ATGAA 7 cut(s) 332, 333, 501, 653, 900, 966, 1043
TspGWI ACGGA 1 cut(s) 807
TspRI CASTG 2 cut(s) 46, 522
VspI ATTAAT 2 cut(s) 156, 389
XagI CCTNNNNNAGG 1 cut(s) 741
XapI RAATTY 2 cut(s) 179, 330
XceI RCATGY 1 cut(s) 424
XmiI GTMKAC 1 cut(s) 789
XspI CTAG 2 cut(s) 809, 842
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.