Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0001743

Physical Location & Seq

Genomic Coordinates Forward (+)

31038859 .. 31043432

Gene Structure 4574 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1245900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2736 bp

ATGATGAGAAAACCCATGGCCGTCATCTGCCACCCACCTGAACCCTTTCTCTTCCTCCTCCTCAAACCCTTCTCTTCCATTTCCTCCGCCGCCGCCGCTCCTCTTCCCCTCTCCAACGAACCGCACGATCTATCCTCCCAGCTCTTCGCCATTCTATCCCGCCCTAATTGGCAGCGCCACCCTTCCCTTAAGCAACTAGCCCCCTCCATATCCCCTTCCCATGTCTCCTCCGTCTTCGCTCTCAAGCTCGACCCCCGAACCGCCCTCGCTTTCTTCAACTGGATAGCTCTTAAACCCGGATACAAGCACACCGTCCATTGCCACTCTTCTCTCCTCAACATTCTCCTCCCCAATGGGTTTCTCCAGGTCGCTGAAAAGATCCGAATTTCCATGATTAAGGCTTCCAGTTCCCCTCAAGATGCCCTTTTCGTGCTCAAGTTTTTGCGGGAGTTAAATGGCGCTGGAGAGTTAAATGGCGCTGGAGAGTTCGAGTTCAAGCTCACCCTCAGGTGCTACAATTTTCTTCTTATGTCATTGTCTAAGTTTTCCCTGCTTCAAGATTTGAAAGCTTTGTATTTGGAGATGTTAGACGATATGGTTTCTCCAAATCTGCATACTTTTAATACCATGATCAATGCTTACTGTAAAGTGGGTAATGTGGCTGAGGCAGACTTGTATTTCAGTAGGATAGGGCAGGCGGGTTTCCACCCCGATACATTTACCTACACTTCTTTGATACTGGGGCATTGTAGGAATAAGGATGTGGATAGTGGTTACAGGGTTTTTAAGCTAATGCCGCAAAAGGGTTGTCAAAGAAATGAAGTTTCCTATACTAATTTGATACATGGGTTTTGCGAGGCTTATCGGATTGATGAGGCTTTAAAGTTGTTTTCTCAAATGGGGGAGGATAATTGTTTTCCAACTGTGCGGACATACACAGTTCTTATTTGCGCCTTGTGTAAATTGGGAAGGAAATTAGAAGCTATGAATCTGTTCAAAGAGATGACGGACAAGGGTTGTGAACCAAATGTTCATACTTATACCGTGCTCATTGATAGCATGTGCAAGGAAAATAAGCTTGATGAGGCACGAAACTTGCTAAACAAGATGTTGGAGAAAAGGTTGGTTCCTAATGTTGTCACGTACAACGCAATGATTGATGGGTACTGCAAGGAGGGAGCAGTTGAGGCTGCACTTGGTATTTTGGCTTTGATGGAATCGAGTAATTGTTATCCAAATGCTCGGACGTACAATGAATTGATTTGTGGGTTTTGTAAAAGGAAAAATATACACCAGGCAATGGCATTGCTTGGTAAGATGCTTGATCGGAAACTCTCACCAAGCCTATATACATACAATTCATTAATCCATGGGCAGTGTACAATGGGTCATTTTGAGAGTGCTTACAGGTTGCTTAATTTGATGAAAGAAAGTGGTTTGGTTCCTGACCGATGGTCTTACAGTTCTCTCATAGACGCTCTCTGTAAGAGGGGCAGACTGGAAGAAGCTCATGCCCTGTTTGATTCTCTCAATGAGAAAGGCATAAAGTCAAATGATGTGATCTTTACTTCTTTGATTGACGGTTATTGCAAGGTTGGGAAAATCGATGATGCCCATTCTTTGTTTGATAGAATGCTTGCAGAAGACTGTTTGCCGAACTCATTCACATACAATACCTTGATAGATGTGTTGTGCAAAGAAGAAAGACTGGAGGAAGCGTTTTCACTGGTGGAAAAGATGTTAAGTATTGGCATCAAAGCTACAACACATACTTATACAATTCTAATTAAACAAATGTTGAAGGAAGGGGACTTTGACCATGCTCGTAGGCTTTTAAACCAGATGGTTAGTTCTGGTAATCAACCTGACCTGTTTACTTACACTACCTTTATTCATGCATATTGCGGCATAGGAAATGTAGAAGAGGCAGAGAAATTGATGATGAAGATGAATGAAGAAGGAATTATAGCAGATTCGTTGACTTACACATTGCTAATTGATGGATATGGACGTATGAGATTAATTGATTGTGCATTTGATGTTCTTAAGCGCATGTTTGATTCTTGTTGTGATCCTTCGCATTTTACATATTCCTTTCTGATCAAACATCTTTCGAATGAAAAGTTGACAGAGACAAATAATAATATGGTGGGAATTGATTTGTTCTCAAAAGTCTCCTTAATTGATATTAGTGATGTGTGGAAGACAATGGATTTTGACATTGCTTTAGAGCTCTTTGAAAAAATGGCTGGACATGGTTGTGCACCCAGTACCAACACTTATGAAAAACTTATAGTAGGTCTTTGCAAAGAGAGGCGCTTGGAAGTAGCCCAGAGGTTATACAGTCATATGAGAGAGAGGAAAATTTCTTCAAGTGAGAATATTTATAATTCTCTTCTTAACTGTTGCTGTACATTGCAAAAGTATGGAGAGGCAGCAACCTTGGTGGATGCGATGATTAAGGATGGTTATTTACCGACACTGGAGTCCTCCACGATGCTTGTATGCGGGCTTCTTGATGAAGAGAAGACTGAGAAGGCAAAAGCAATTTTTCGTACTTTGCTTCTTTGCGAATATAATCACGATGAAGTAGCTTGGAAAGTTCTCTTTGATGGTTTACTTAAGAGGGGTTTTGTCAATATATGCTCTGAGCTCATAAGCATCATGGAGAAAATGGGTTGCCAGCTTCATCCTCAGACATATTCAATGTTGATTGAGGGAATGGATGGTCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

912

Amino Acids

103.03

Weight (kDa)

6.32

Isoelectric Point (pI)

31.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	171 - 216	6.2e-07	PPR repeat family
PPR_1	PF12854	199 - 223	1.2e-07	PPR repeat
PPR_2	PF13041	202 - 251	2e-12	PPR repeat family
PPR_1	PF12854	234 - 265	8e-08	PPR repeat
PPR_2	PF13041	237 - 285	6.5e-14	PPR repeat family
PPR_long	PF17177	253 - 324	4.2e-06	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	256 - 363	6.7e-07	Fungal tetratrico peptide repeats
PPR_1	PF12854	268 - 300	6.7e-10	PPR repeat
PPR_2	PF13041	273 - 321	1.2e-15	PPR repeat family
PPR	PF01535	275 - 305	4.1e-06	PPR repeat
PPR_1	PF12854	304 - 336	6.1e-09	PPR repeat
PPR	PF01535	311 - 340	1.6e-07	PPR repeat
PPR_2	PF13041	315 - 356	2.5e-14	PPR repeat family
PPR_long	PF17177	328 - 418	4.4e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	331 - 389	8.9e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	338 - 370	1.3e-12	PPR repeat
PPR	PF01535	346 - 374	2.5e-06	PPR repeat
PPR_2	PF13041	347 - 388	4.8e-12	PPR repeat family
PPR_1	PF12854	374 - 406	6.3e-10	PPR repeat
PPR_2	PF13041	377 - 426	2e-17	PPR repeat family
PPR	PF01535	380 - 410	1.2e-07	PPR repeat
PPR_long	PF17177	394 - 511	1.5e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	402 - 456	7.7e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	409 - 440	3.3e-08	PPR repeat
PPR_2	PF13041	416 - 460	1.6e-12	PPR repeat family
PPR_3	PF13812	436 - 496	1.1e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	445 - 476	1.4e-07	PPR repeat
PPR_2	PF13041	447 - 496	3.1e-15	PPR repeat family
PPR_1	PF12854	479 - 508	1.4e-09	PPR repeat
PPR_2	PF13041	486 - 519	2.4e-06	PPR repeat family
PPR	PF01535	486 - 515	6.2e-07	PPR repeat
PPR_long	PF17177	500 - 603	2.5e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	506 - 564	1.9e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	513 - 545	1.8e-10	PPR repeat
PPR_2	PF13041	518 - 566	1.8e-16	PPR repeat family
PPR	PF01535	520 - 550	5.3e-08	PPR repeat
PPR_1	PF12854	549 - 580	2.9e-12	PPR repeat
PPR_2	PF13041	552 - 599	5.2e-15	PPR repeat family
PPR	PF01535	555 - 585	6.7e-08	PPR repeat
TPR_24	PF23276	555 - 645	2.7e-06	Fungal tetratrico peptide repeats
PPR_long	PF17177	585 - 698	3.1e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	594 - 635	1.8e-10	PPR repeat family
PPR_3	PF13812	610 - 671	2.9e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	621 - 650	3.5e-08	PPR repeat
PPR_2	PF13041	622 - 670	2.9e-13	PPR repeat family
PPR	PF01535	625 - 655	2.1e-06	PPR repeat
PPR_3	PF13812	646 - 704	3.3e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	658 - 705	3.3e-08	PPR repeat family
PPR_long	PF17177	731 - 856	9.1e-08	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	760 - 804	2.3e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001743)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77340 AT5G65560 AT5G65560 AT5G65560
fragaria_vesca	FvH4_1g06430 FvH4_2g11090 FvH4_2g11090 FvH4_2g11090 FvH4_2g11090 FvH4_2g11090 FvH4_7g04271 FvH4_7g04271
malus_domestica	MD03G1170600.v1.1 MD08G1245900.v1.1 MD11G1188000.v1.1 MD15G1441200.v1.1
prunus_persica	Prupe.1G583300_v2.0.a1 Prupe.4G256300_v2.0.a1
pyrus_communis	pycom03g12750 pycom08g21340 pycom15g38890 pycom15g38910
rosa_chinensis	RchiOBHm_Chr1g0329241 RchiOBHm_Chr2g0092171
rosa_laevigata	RLG00000016282 RLG00000029907
rosa_multiflora	Rmu_co8315275.1_g000001 Rmu_sc0000203.1_g000005 Rmu_sc0000524.1_g000014 Rmu_sc0010272.1_g000004 Rmu_sc0013552.1_g000009
rosa_roxburghii	Rroxscaffold_2G00149410 Rroxscaffold_4G00321300
rosa_rugosa	Rorug01G0077300 Rorug01G0077300 Rorug02G0023500
rosa_samantha	Rh1AG096400 Rh1BG077100 Rh1CG093100 Rh1CG107900 Rh1DG099700 Rh1DG116600 Rh2AG069100 Rh2BG068600 Rh2CG069700 Rh2DG067700
rosa_wichuraiana	Rw1G007460 Rw2G006310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2388
AasI	GACNNNNNNGTC	1 cut(s)	2485
AccB7I	CCANNNNNTGG	1 cut(s)	1300
AccBSI	CCGCTC	1 cut(s)	98
AclWI	GGATC	2 cut(s)	373, 2066
AcoI	YGGCCR	1 cut(s)	18
AcsI	RAATTY	2 cut(s)	384, 2364
AfaI	GTAC	7 cut(s)	1145, 1166, 1250, 1381, 2272, 2413, 2557
AfiI	CCNNNNNNNGG	2 cut(s)	803, 1300
AflII	CTTAAG	3 cut(s)	188, 2045, 2621
AgsI	TTSAA	9 cut(s)	277, 496, 557, 565, 997, 1801, 2240, 2373, 2706
AhdI	GACNNNNNGTC	1 cut(s)	1453
AjnI	CCWGG	2 cut(s)	363, 1293
Alw21I	GWGCWC	5 cut(s)	435, 1050, 2235, 2266, 2655
Alw26I	GTCTC	3 cut(s)	229, 2126, 2179
Alw44I	GTGCAC	1 cut(s)	2262
AlwI	GGATC	2 cut(s)	373, 2066
AoxI	GGCC	1 cut(s)	18
ApaLI	GTGCAC	1 cut(s)	2262
ApeKI	GCWGC	3 cut(s)	172, 1190, 2435
ApoI	RAATTY	2 cut(s)	384, 2364
ArsI	GACNNNNNNTTYG	2 cut(s)	793, 825
AseI	ATTAAT	2 cut(s)	1364, 2021
Asp700I	GAANNNNTTC	3 cut(s)	45, 992, 1661
AspLEI	GCGC	6 cut(s)	177, 461, 479, 953, 2052, 2319
AspS9I	GGNCC	1 cut(s)	2729
AsuC2I	CCSGG	1 cut(s)	297
AsuHPI	GGTGA	2 cut(s)	493, 1329
AsuII	TTCGAA	1 cut(s)	2114
AvaII	GGWCC	1 cut(s)	2729
AxyI	CCTNAGG	1 cut(s)	506
BaeGI	GKGCMC	1 cut(s)	2266
BaeI	ACNNNNGTAYC	2 cut(s)	833, 866
BanII	GRGCYC	2 cut(s)	2235, 2655
BarI	GAAGNNNNNNTAC	2 cut(s)	2496, 2528
BbsI	GAAGAC	4 cut(s)	226, 1650, 2210, 2534
Bbv12I	GWGCWC	5 cut(s)	435, 1050, 2235, 2266, 2655
BbvCI	CCTCAGC	1 cut(s)	663
BbvI	GCAGC	3 cut(s)	184, 1177, 2447
BccI	CCATC	8 cut(s)	1154, 1207, 1446, 1837, 1994, 2459, 2606, 2720
BceAI	ACGGC	1 cut(s)	5
BciT130I	CCWGG	2 cut(s)	365, 1295
BciVI	GTATCC	1 cut(s)	293
BclI	TGATCA	2 cut(s)	630, 2100
BcnI	CCSGG	1 cut(s)	297
BcoDI	GTCTC	3 cut(s)	229, 2126, 2179
BfaI	CTAG	1 cut(s)	197
BfoI	RGCGCY	4 cut(s)	178, 462, 480, 2320
BfrI	CTTAAG	3 cut(s)	188, 2045, 2621
BfuI	GTATCC	1 cut(s)	293
BisI	GCNGC	8 cut(s)	90, 93, 96, 173, 797, 1191, 1906, 2436
BlsI	GCNGC	8 cut(s)	91, 94, 97, 174, 798, 1192, 1907, 2437
Bme1390I	CCNGG	3 cut(s)	297, 365, 1295
Bme18I	GGWCC	1 cut(s)	2729
BmeRI	GACNNNNNGTC	1 cut(s)	1453
BmgT120I	GGNCC	1 cut(s)	2729
BmiI	GGNNCC	2 cut(s)	1128, 1443
BmrFI	CCNGG	3 cut(s)	297, 365, 1295
BmrI	ACTGGG	2 cut(s)	749, 2262
BmsI	GCATC	7 cut(s)	409, 1308, 1600, 1761, 2440, 2487, 2670
BmuI	ACTGGG	2 cut(s)	749, 2262
BpiI	GAAGAC	4 cut(s)	226, 1650, 2210, 2534
BpmI	CTGGAG	5 cut(s)	347, 483, 501, 1730, 2504
Bpu10I	CCTNAGC	1 cut(s)	663
Bpu14I	TTCGAA	1 cut(s)	2114
BpuEI	CTTGAG	3 cut(s)	227, 399, 419
BpuMI	CCSGG	1 cut(s)	297
Bsa29I	ATCGAT	1 cut(s)	1605
BsaAI	YACGTR	1 cut(s)	1143
BsaJI	CCNNGG	3 cut(s)	15, 1369, 2442
BsaXI	ACNNNNNCTCC	4 cut(s)	1106, 1136, 1703, 1733
Bsc4I	CCNNNNNNNGG	2 cut(s)	803, 1300
Bse1I	ACTGG	8 cut(s)	284, 405, 744, 1503, 1713, 1731, 2268, 2487
Bse21I	CCTNAGG	1 cut(s)	506
Bse3DI	GCAATG	7 cut(s)	316, 1158, 1304, 1305, 1988, 2220, 2414
BseBI	CCWGG	2 cut(s)	365, 1295
BseCI	ATCGAT	1 cut(s)	1605
BseDI	CCNNGG	3 cut(s)	15, 1369, 2442
BseGI	GGATG	5 cut(s)	766, 2455, 2470, 2689, 2731
BseLI	CCNNNNNNNGG	2 cut(s)	803, 1300
BseMI	GCAATG	7 cut(s)	316, 1158, 1304, 1305, 1988, 2220, 2414
BseMII	CTCAG	5 cut(s)	520, 654, 2523, 2640, 2708
BseNI	ACTGG	8 cut(s)	284, 405, 744, 1503, 1713, 1731, 2268, 2487
BseRI	GAGGAG	6 cut(s)	47, 50, 90, 217, 323, 335
BseSI	GKGCMC	1 cut(s)	2266
BseXI	GCAGC	3 cut(s)	184, 1177, 2447
BseYI	CCCAGC	1 cut(s)	138
BsgI	GTGCAG	1 cut(s)	1176
BshFI	GGCC	1 cut(s)	20
BshVI	ATCGAT	1 cut(s)	1605
BsiHKAI	GWGCWC	5 cut(s)	435, 1050, 2235, 2266, 2655
BsiSI	CCGG	1 cut(s)	297
BslFI	GGGAC	1 cut(s)	1823
BslI	CCNNNNNNNGG	2 cut(s)	803, 1300
BsmAI	GTCTC	3 cut(s)	229, 2126, 2179
BsmFI	GGGAC	1 cut(s)	1823
BsmI	GAATGC	1 cut(s)	1638
BsnI	GGCC	1 cut(s)	20
Bsp119I	TTCGAA	1 cut(s)	2114
Bsp1286I	GDGCHC	5 cut(s)	435, 1050, 2235, 2266, 2655
Bsp1407I	TGTACA	2 cut(s)	1379, 2411
Bsp143I	GATC	7 cut(s)	127, 378, 630, 1324, 1560, 2071, 2100
Bsp19I	CCATGG	2 cut(s)	15, 1369
BspANI	GGCC	1 cut(s)	20
BspCNI	CTCAG	5 cut(s)	519, 655, 2524, 2641, 2707
BspDI	ATCGAT	1 cut(s)	1605
BspLI	GGNNCC	2 cut(s)	1128, 1443
BspPI	GGATC	2 cut(s)	373, 2066
BspQI	GCTCTTC	1 cut(s)	149
BspT104I	TTCGAA	1 cut(s)	2114
BspTI	CTTAAG	3 cut(s)	188, 2045, 2621
BsrBI	CCGCTC	1 cut(s)	98
BsrDI	GCAATG	7 cut(s)	316, 1158, 1304, 1305, 1988, 2220, 2414
BsrGI	TGTACA	2 cut(s)	1379, 2411
BsrI	ACTGG	8 cut(s)	284, 405, 744, 1503, 1713, 1731, 2268, 2487
BssECI	CCNNGG	3 cut(s)	15, 1369, 2442
BssMI	GATC	7 cut(s)	127, 378, 630, 1324, 1560, 2071, 2100
BssT1I	CCWWGG	3 cut(s)	15, 1369, 2442
Bst2UI	CCWGG	2 cut(s)	365, 1295
Bst6I	CTCTTC	8 cut(s)	56, 79, 108, 149, 331, 1917, 2400, 2517
BstAFI	CTTAAG	3 cut(s)	188, 2045, 2621
BstAUI	TGTACA	2 cut(s)	1379, 2411
BstBAI	YACGTR	1 cut(s)	1143
BstBI	TTCGAA	1 cut(s)	2114
BstC8I	GCNNGC	4 cut(s)	696, 1638, 2510, 2684
BstDEI	CTNAG	6 cut(s)	506, 540, 663, 2532, 2649, 2694
BstDSI	CCRYGG	2 cut(s)	15, 1369
BstF5I	GGATG	5 cut(s)	766, 2455, 2470, 2689, 2731
BstH2I	RGCGCY	4 cut(s)	178, 462, 480, 2320
BstHHI	GCGC	6 cut(s)	177, 461, 479, 953, 2052, 2319
BstKTI	GATC	7 cut(s)	130, 381, 633, 1327, 1563, 2074, 2103
BstMAI	GTCTC	3 cut(s)	229, 2126, 2179
BstMBI	GATC	7 cut(s)	127, 378, 630, 1324, 1560, 2071, 2100
BstMWI	GCNNNNNNNGC	3 cut(s)	95, 796, 1187
BstNI	CCWGG	2 cut(s)	365, 1295
BstNSI	RCATGY	2 cut(s)	1063, 2056
BstSCI	CCNGG	3 cut(s)	295, 363, 1293
BstSLI	GKGCMC	1 cut(s)	2266
BstV1I	GCAGC	3 cut(s)	184, 1177, 2447
BstV2I	GAAGAC	4 cut(s)	226, 1650, 2210, 2534
BstX2I	RGATCY	1 cut(s)	378
BstYI	RGATCY	1 cut(s)	378
Bsu15I	ATCGAT	1 cut(s)	1605
Bsu36I	CCTNAGG	1 cut(s)	506
BsuI	GTATCC	1 cut(s)	293
BsuRI	GGCC	1 cut(s)	20
BsuTUI	ATCGAT	1 cut(s)	1605
BtgI	CCRYGG	2 cut(s)	15, 1369
BtgZI	GCGATG	1 cut(s)	2468
BtsCI	GGATG	5 cut(s)	766, 2455, 2470, 2689, 2731
BtsI	GCAGTG	1 cut(s)	1382
BtsIMutI	CAGTG	3 cut(s)	1382, 1724, 2480
Cac8I	GCNNGC	4 cut(s)	696, 1638, 2510, 2684
CfoI	GCGC	6 cut(s)	177, 461, 479, 953, 2052, 2319
Cfr13I	GGNCC	1 cut(s)	2729
ClaI	ATCGAT	1 cut(s)	1605
CseI	GACGC	1 cut(s)	1484
Csp6I	GTAC	7 cut(s)	1144, 1165, 1249, 1380, 2271, 2412, 2556
CspCI	CAANNNNNGTGG	4 cut(s)	2427, 2462, 2482, 2517
CviQI	GTAC	7 cut(s)	1144, 1165, 1249, 1380, 2271, 2412, 2556
DdeI	CTNAG	6 cut(s)	506, 540, 663, 2532, 2649, 2694
DpnI	GATC	7 cut(s)	129, 380, 632, 1326, 1562, 2073, 2102
DpnII	GATC	7 cut(s)	127, 378, 630, 1324, 1560, 2071, 2100
DraI	TTTAAA	2 cut(s)	882, 1836
DrdI	GACNNNNNNGTC	1 cut(s)	2485
DriI	GACNNNNNGTC	1 cut(s)	1453
DseDI	GACNNNNNNGTC	1 cut(s)	2485
EaeI	YGGCCR	1 cut(s)	18
Eam1104I	CTCTTC	8 cut(s)	56, 79, 108, 149, 331, 1917, 2400, 2517
Eam1105I	GACNNNNNGTC	1 cut(s)	1453
EarI	CTCTTC	8 cut(s)	56, 79, 108, 149, 331, 1917, 2400, 2517
EciI	GGCGGA	1 cut(s)	76
Ecl136II	GAGCTC	2 cut(s)	2233, 2653
Eco130I	CCWWGG	3 cut(s)	15, 1369, 2442
Eco24I	GRGCYC	2 cut(s)	2235, 2655
Eco47I	GGWCC	1 cut(s)	2729
Eco53kI	GAGCTC	2 cut(s)	2233, 2653
Eco81I	CCTNAGG	1 cut(s)	506
EcoICRI	GAGCTC	2 cut(s)	2233, 2653
EcoRII	CCWGG	2 cut(s)	363, 1293
EcoT14I	CCWWGG	3 cut(s)	15, 1369, 2442
EcoT22I	ATGCAT	1 cut(s)	1900
EcoT38I	GRGCYC	2 cut(s)	2235, 2655
ErhI	CCWWGG	3 cut(s)	15, 1369, 2442
FalI	AAGNNNNNCTT	2 cut(s)	408, 440
FaqI	GGGAC	1 cut(s)	1823
FauI	CCCGC	4 cut(s)	167, 438, 691, 2501
FauNDI	CATATG	1 cut(s)	2349
FbaI	TGATCA	2 cut(s)	630, 2100
Fnu4HI	GCNGC	8 cut(s)	90, 93, 96, 173, 797, 1191, 1906, 2436
FokI	GGATG	4 cut(s)	773, 2462, 2477, 2676
FriOI	GRGCYC	2 cut(s)	2235, 2655
Fsp4HI	GCNGC	8 cut(s)	90, 93, 96, 173, 797, 1191, 1906, 2436
FspBI	CTAG	1 cut(s)	197
GlaI	GCGC	6 cut(s)	176, 460, 478, 952, 2051, 2318
GluI	GCNGC	8 cut(s)	90, 93, 96, 173, 797, 1191, 1906, 2436
GsaI	CCCAGC	1 cut(s)	142
GsuI	CTGGAG	5 cut(s)	347, 483, 501, 1730, 2504
HaeII	RGCGCY	4 cut(s)	178, 462, 480, 2320
HaeIII	GGCC	1 cut(s)	20
HapII	CCGG	1 cut(s)	297
HgaI	GACGC	1 cut(s)	1484
HhaI	GCGC	6 cut(s)	177, 461, 479, 953, 2052, 2319
Hin6I	GCGC	6 cut(s)	175, 459, 477, 951, 2050, 2317
HinP1I	GCGC	6 cut(s)	175, 459, 477, 951, 2050, 2317
HincII	GTYRAC	2 cut(s)	1980, 2127
HindII	GTYRAC	2 cut(s)	1980, 2127
HindIII	AAGCTT	2 cut(s)	567, 1076
HinfI	GANTC	6 cut(s)	988, 1217, 1523, 1973, 2060, 2486
HpaII	CCGG	1 cut(s)	297
HphI	GGTGA	2 cut(s)	493, 1329
Hpy166II	GTNNAC	7 cut(s)	1022, 1380, 1875, 1980, 2127, 2264, 2618
Hpy188I	TCNGA	7 cut(s)	383, 867, 1245, 1329, 2100, 2650, 2697
Hpy188III	TCNNGA	5 cut(s)	416, 557, 1445, 2516, 2582
Hpy8I	GTNNAC	7 cut(s)	1022, 1380, 1875, 1980, 2127, 2264, 2618
HpyAV	CCTTC	9 cut(s)	79, 192, 225, 963, 1795, 1799, 1952, 2085, 2530
HpyCH4IV	ACGT	3 cut(s)	1142, 1247, 2011
HpyF10VI	GCNNNNNNNGC	3 cut(s)	95, 796, 1187
HpyF3I	CTNAG	6 cut(s)	506, 540, 663, 2532, 2649, 2694
HpySE526I	ACGT	3 cut(s)	1142, 1247, 2011
HspAI	GCGC	6 cut(s)	175, 459, 477, 951, 2050, 2317
Ksp22I	TGATCA	2 cut(s)	630, 2100
Kzo9I	GATC	7 cut(s)	127, 378, 630, 1324, 1560, 2071, 2100
LguI	GCTCTTC	1 cut(s)	149
LmnI	GCTCC	2 cut(s)	103, 1178
Lsp1109I	GCAGC	3 cut(s)	184, 1177, 2447
LweI	GCATC	7 cut(s)	409, 1308, 1600, 1761, 2440, 2487, 2670
MaeI	CTAG	1 cut(s)	197
MaeII	ACGT	3 cut(s)	1142, 1247, 2011
MaeIII	GTNAC	2 cut(s)	773, 1138
MalI	GATC	7 cut(s)	129, 380, 632, 1326, 1562, 2073, 2102
MbiI	CCGCTC	1 cut(s)	98
MboI	GATC	7 cut(s)	127, 378, 630, 1324, 1560, 2071, 2100
MflI	RGATCY	1 cut(s)	378
MhlI	GDGCHC	5 cut(s)	435, 1050, 2235, 2266, 2655
MlyI	GAGTC	1 cut(s)	2495
MmeI	TCCRAC	3 cut(s)	138, 944, 1092
Mph1103I	ATGCAT	1 cut(s)	1900
MroXI	GAANNNNTTC	3 cut(s)	45, 992, 1661
MslI	CAYNNNNRTG	1 cut(s)	2259
MspCI	CTTAAG	3 cut(s)	188, 2045, 2621
MspI	CCGG	1 cut(s)	297
MspR9I	CCNGG	3 cut(s)	297, 365, 1295
Mva1269I	GAATGC	1 cut(s)	1638
MvaI	CCWGG	2 cut(s)	365, 1295
MwoI	GCNNNNNNNGC	3 cut(s)	95, 796, 1187
NciI	CCSGG	1 cut(s)	297
NcoI	CCATGG	2 cut(s)	15, 1369
NdeI	CATATG	1 cut(s)	2349
NdeII	GATC	7 cut(s)	127, 378, 630, 1324, 1560, 2071, 2100
NlaIV	GGNNCC	2 cut(s)	1128, 1443
NmuCI	GTSAC	1 cut(s)	1138
NsiI	ATGCAT	1 cut(s)	1900
NspI	RCATGY	2 cut(s)	1063, 2056
NspV	TTCGAA	1 cut(s)	2114
PciSI	GCTCTTC	1 cut(s)	149
PcsI	WCGNNNNNNNCGW	1 cut(s)	123
PctI	GAATGC	1 cut(s)	1638
PdmI	GAANNNNTTC	3 cut(s)	45, 992, 1661
PfeI	GAWTC	5 cut(s)	988, 1217, 1523, 1973, 2060
PflMI	CCANNNNNTGG	1 cut(s)	1300
PkrI	GCNGC	8 cut(s)	91, 94, 97, 174, 798, 1192, 1907, 2437
PleI	GAGTC	1 cut(s)	2494
PpsI	GAGTC	1 cut(s)	2494
Ppu21I	YACGTR	1 cut(s)	1143
PshBI	ATTAAT	2 cut(s)	1364, 2021
PsiI	TTATAA	1 cut(s)	2388
Psp124BI	GAGCTC	2 cut(s)	2235, 2655
Psp6I	CCWGG	2 cut(s)	363, 1293
PspFI	CCCAGC	1 cut(s)	138
PspGI	CCWGG	2 cut(s)	363, 1293
PspN4I	GGNNCC	2 cut(s)	1128, 1443
PspPI	GGNCC	1 cut(s)	2729
PsuI	RGATCY	1 cut(s)	378
RsaI	GTAC	7 cut(s)	1145, 1166, 1250, 1381, 2272, 2413, 2557
RsaNI	GTAC	7 cut(s)	1144, 1165, 1249, 1380, 2271, 2412, 2556
RseI	CAYNNNNRTG	1 cut(s)	2259
SacI	GAGCTC	2 cut(s)	2235, 2655
SapI	GCTCTTC	1 cut(s)	149
SatI	GCNGC	8 cut(s)	90, 93, 96, 173, 797, 1191, 1906, 2436
Sau3AI	GATC	7 cut(s)	127, 378, 630, 1324, 1560, 2071, 2100
Sau96I	GGNCC	1 cut(s)	2729
SchI	GAGTC	1 cut(s)	2495
ScrFI	CCNGG	3 cut(s)	297, 365, 1295
SduI	GDGCHC	5 cut(s)	435, 1050, 2235, 2266, 2655
SfaNI	GCATC	7 cut(s)	409, 1308, 1600, 1761, 2440, 2487, 2670
SfuI	TTCGAA	1 cut(s)	2114
SinI	GGWCC	1 cut(s)	2729
SmiMI	CAYNNNNRTG	1 cut(s)	2259
SmlI	CTYRAG	6 cut(s)	188, 242, 414, 434, 2045, 2621
SmoI	CTYRAG	6 cut(s)	188, 242, 414, 434, 2045, 2621
SspI	AATATT	1 cut(s)	2383
SspMI	CTAG	1 cut(s)	197
SstI	GAGCTC	2 cut(s)	2235, 2655
StyD4I	CCNGG	3 cut(s)	295, 363, 1293
StyI	CCWWGG	3 cut(s)	15, 1369, 2442
TaiI	ACGT	3 cut(s)	1145, 1250, 2014
TaqI	TCGA	5 cut(s)	249, 489, 1220, 1605, 2114
TaqII	GACCGA	1 cut(s)	1464
TatI	WGTACW	2 cut(s)	1379, 2411
TauI	GCSGC	5 cut(s)	92, 95, 98, 799, 1908
TfiI	GAWTC	5 cut(s)	988, 1217, 1523, 1973, 2060
TscAI	CASTG	3 cut(s)	1382, 1731, 2487
TseFI	GTSAC	1 cut(s)	1138
TseI	GCWGC	3 cut(s)	172, 1190, 2435
Tsp45I	GTSAC	1 cut(s)	1138
TspGWI	ACGGA	2 cut(s)	220, 1022
TspRI	CASTG	3 cut(s)	1382, 1731, 2487
Van91I	CCANNNNNTGG	1 cut(s)	1300
Vha464I	CTTAAG	3 cut(s)	188, 2045, 2621
VneI	GTGCAC	1 cut(s)	2262
VpaK11BI	GGWCC	1 cut(s)	2729
VspI	ATTAAT	2 cut(s)	1364, 2021
XapI	RAATTY	2 cut(s)	384, 2364
XceI	RCATGY	2 cut(s)	1063, 2056
XmnI	GAANNNNTTC	3 cut(s)	45, 992, 1661
XspI	CTAG	1 cut(s)	197
Zsp2I	ATGCAT	1 cut(s)	1900

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.