Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001743

Physical Location & Seq

Genomic Coordinates Forward (+)

23143852 .. 23150245

Gene Structure 6394 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1170600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2703 bp

ATGATGAGAAGAGAACTGACGGTGGTGATCACCACGCGCCAAACCCTCCTCCTCCTCCTCAAACCCTTGTCTTCCCTCGCCACCGCAACACCGCCGCCCGATATCTCTTCCGAGCTCTTCGCCGTCCTCTCTCACCGGAATTGGCAGAAACATCCTTCCCTCAGAAACCTAATCCCCTCCATATCCCCTTCCCATGTCTCTTCCCTCTTCGCCCGCAACCTCCACCCCCAAATTGCCCTCTCCTTCTTCCGCTGGATCGCCGAAATCCCCCGATACAAGCACACCGTCCACTCTCATTCCTCATTGCTCCTCAACATTCTGCTTCCCAATCGCTGGCTCGATGCCGCCGAGAAGATCCGGATTTCGATGCTCAAGGCCTGCGATTCGCCTGAGGATGCCCACTTTGTGCTCGACCTGTTGCGCCGGATGAACGACGGCGGTTCTGGTTTCGTCGAGTTCAAGCTCACTCTCAGGTCCTACAACTGGCTCCTAATATCTGTGTCCAAGTTTTTACTAATTGATGATGTGAAAACTGTGTTTTTGGAAATGTTGGCCGACAAGATTCCGCCGGATATTGTTACGTTGAATACCATGGTGGTTGCTTATTGTAAATTGGGAAATGTGGCCGGGGCCAATTTGTATGTCAGTAAGATAGTGCAGGCAGGTTTTAACCCCACTAGTTTTACATACAGTTCATTGATAATGGGGCATTGTAGGAACAAGGATGTGGATAATGCTTTTAAGATTTTTGAGGTAATGCCGGATAAGGGCTGTCCAAGAGACGTGGTTTCGTATAATTATTTAATTCGGGGGTTTTGTGAGGCTCATCGCATTGATGAGGCACTCAAGTTGTTCTCTCACATGGGGCAGGGGGGCGATAATTGTTTTCCTAATGTGAGAACATATACGGTTCTTATTCATGCATTGTGTGGATCAAGAAGGAAACTAGAAGCAATTAATCTATTTAAAGAGATGATAGAGAAGGGTTGTGAACCCAATGTATATACTTATTGTGTGCTCATTGATAGCATGTGTAAGGAAAATAAGCTTGATTATGCTAGAAAGGTGCTAAATCAGATGCTGGAGAAAGGATTGAGTCCTAATGTTGTCACATACAATGCATTAATAAATGGGTATTGCAAGGAGGGAACAGTTGAGGCTGCACTTGAGATTTTGGCTTTGATGGAATCAAATGTTTGTTGCCCAGATGTTCACACATTCAGTGTATTGATTGACGGGTTGTGTAAAAGGAATAATGTGCACCAGGCAATGGCACTGCTTAACAACATGCTTGAACGGAAGCTCTCACCAAACTTGGTTACATATAACTCATTACTCCATGGCCAGTGTAAAGGAGGTCATTTCGATAGTGCTCATAGGTTGCTTAATTTGATGACTCATAGTGGTTTGGCTCCTGACCAGATTACTTACTGTATTTTCATCGATGGTCTCTGTAAGTGGAAGAGATTACAGGAAGCTCAAACCATGTTTGATACTCTCAAGAAGAATGGCATCAAGTCAAGTACAGAGATGTTTACTGCTTTAATTGATGGTTACTGCAAGATTGGGAAAATCGATGATGCCCTTTCTCTATTTGATTGTATGCTTGCTGAGGATTGTTTGCCAAATTCATATGCTATCAATGCCTTGATACATGGGCTTTTCAAAGAGAAAAAAATGATAGAAGCATCCTCAGTCATGGAAAAGATGTTAAGTATAGGTGTGAAGCCTACAGTCCCTCATTATAATATTTTAATTAATCATGTTCTGAAAGAAGGTGACTTTGATCGTGCTCATCAGCTTCTCAATAAAATGGTCTCTGTCGGTAACAAGCCTACTCTAATTACTTACAACACATTAATTCAGGCATATTGCGCGTTAGGGAATATAAATGAAGCGGAGAAGCTGATGAGTAAGATGAATGGAGAAGGAATTTCTGCAGATTCGTTGACTTACACATTTTTAATTGATGGATATGTCCGTATGGGATTAATTGATTGTGCGTTTGATGTTCTTAAGCGCATGTTTGATTCTGGTTGTGAGCCTTGTCATCGTACCTATTCCTTTCTAATCAAATATCTCTCAAATAAGAAGTTCGTCATGACAAACAGCAAAGCAGTGGGTCTCGATATAATGTTGAATGTCTCTGGAAATATCACTGATATATGGAAGACCATGGATTTTGAAATTGCTTTGGAGCTATTTGAGAAGATGGTTGGACATGGTTGTGCTCCCAATATGAACACCTATGGCGAGCTTATAGTAAGACTTTGCAAAGAGAGAAACTTGGAAGTGGCCCAGAGGTTATATGATCATATGAGAGATATGGGTATTTCTCCCAACGAAGATATTTATAATTCTCTTGTTAATTGTTGCTGTGAGATGCATGTGTATGGAGAAGCAGCAATTCTAGTGCATACTATGATTGAGCATGGTTATTTACCAGCATTAGAGTCGTGCAAGTTGCTTGTAAGTGGCCTTTTTGATGAAGAGAATGACGAGAAGGCTAAGGAAGTTTTTTGTAGCATGCTTCGCTGTGGGTATAACTATGATGAAGTAGCTTGGAAACTTCTCCTTGATGGTTTGATTAAGAGGGGCCATTTAAATAGAGGATCAGAGCTGCTAACAGTCATGGAGAAAATGGGTTGCCAGCTTCATCCTGAGACAAATAGAATGTTGACTGAGGGACTTAATGGGACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

901

Amino Acids

101.5

Weight (kDa)

6.76

Isoelectric Point (pI)

32.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	190 - 239	4.5e-09	PPR repeat family
PPR_3	PF13812	217 - 270	3.2e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	222 - 253	3.3e-10	PPR repeat
PPR_2	PF13041	225 - 273	1.2e-14	PPR repeat family
PPR	PF01535	228 - 258	3.4e-07	PPR repeat
PPR_1	PF12854	256 - 288	2.6e-10	PPR repeat
PPR_2	PF13041	260 - 310	3.3e-15	PPR repeat family
PPR_long	PF17177	280 - 398	2.8e-08	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	294 - 325	3.4e-07	PPR repeat
PPR_3	PF13812	296 - 343	2.7e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	297 - 346	3.7e-17	PPR repeat family
PPR	PF01535	301 - 330	3.4e-06	PPR repeat
PPR_1	PF12854	328 - 360	2.4e-11	PPR repeat
PPR_2	PF13041	340 - 373	1.2e-08	PPR repeat family
TPR_24	PF23276	353 - 432	2.3e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	355 - 412	1.9e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	363 - 396	2.1e-12	PPR repeat
PPR_2	PF13041	367 - 416	8.4e-19	PPR repeat family
PPR	PF01535	370 - 398	5e-07	PPR repeat
PPR_1	PF12854	400 - 430	2.5e-10	PPR repeat
PPR_2	PF13041	410 - 451	2.5e-12	PPR repeat family
PPR_1	PF12854	435 - 465	2.2e-08	PPR repeat
PPR_2	PF13041	437 - 486	3.7e-16	PPR repeat family
PPR_1	PF12854	469 - 499	9.9e-07	PPR repeat
TPR_24	PF23276	493 - 603	3.3e-07	Fungal tetratrico peptide repeats
PPR_1	PF12854	504 - 535	4.5e-08	PPR repeat
PPR_long	PF17177	509 - 692	5.1e-16	Pentacotripeptide-repeat region of PRORP
PPR	PF01535	511 - 540	1.7e-07	PPR repeat
PPR_2	PF13041	511 - 554	1.9e-10	PPR repeat family
PPR_3	PF13812	531 - 588	3.7e-06	Pentatricopeptide repeat domain
PPR_3	PF13812	565 - 624	1.4e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	577 - 625	4.8e-13	PPR repeat family
PPR_1	PF12854	611 - 640	1.1e-09	PPR repeat
PPR_2	PF13041	612 - 660	9.5e-15	PPR repeat family
PPR	PF01535	615 - 645	1.8e-07	PPR repeat
PPR_3	PF13812	635 - 694	3.9e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	644 - 675	1.9e-06	PPR repeat
PPR_2	PF13041	648 - 694	6e-09	PPR repeat family
PPR_long	PF17177	717 - 894	2.6e-11	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	734 - 793	1.8e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	749 - 793	2.8e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001743)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77340 AT5G65560 AT5G65560 AT5G65560
fragaria_vesca	FvH4_1g06430 FvH4_2g11090 FvH4_2g11090 FvH4_2g11090 FvH4_2g11090 FvH4_2g11090 FvH4_7g04271 FvH4_7g04271
malus_domestica	MD03G1170600.v1.1 MD08G1245900.v1.1 MD11G1188000.v1.1 MD15G1441200.v1.1
prunus_persica	Prupe.1G583300_v2.0.a1 Prupe.4G256300_v2.0.a1
pyrus_communis	pycom03g12750 pycom08g21340 pycom15g38890 pycom15g38910
rosa_chinensis	RchiOBHm_Chr1g0329241 RchiOBHm_Chr2g0092171
rosa_laevigata	RLG00000016282 RLG00000029907
rosa_multiflora	Rmu_co8315275.1_g000001 Rmu_sc0000203.1_g000005 Rmu_sc0000524.1_g000014 Rmu_sc0010272.1_g000004 Rmu_sc0013552.1_g000009
rosa_roxburghii	Rroxscaffold_2G00149410 Rroxscaffold_4G00321300
rosa_rugosa	Rorug01G0077300 Rorug01G0077300 Rorug02G0023500
rosa_samantha	Rh1AG096400 Rh1BG077100 Rh1CG093100 Rh1CG107900 Rh1DG099700 Rh1DG116600 Rh2AG069100 Rh2BG068600 Rh2CG069700 Rh2DG067700
rosa_wichuraiana	Rw1G007460 Rw2G006310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1746, 2355
Acc36I	ACCTGC	1 cut(s)	653
AccB7I	CCANNNNNTGG	2 cut(s)	333, 1270
AccII	CGCG	2 cut(s)	37, 1877
AccIII	TCCGGA	1 cut(s)	357
AciI	CCGC	9 cut(s)	84, 92, 95, 214, 250, 345, 438, 566, 1898
AclWI	GGATC	4 cut(s)	263, 349, 940, 2620
AcoI	YGGCCR	3 cut(s)	552, 624, 1342
AcsI	RAATTY	2 cut(s)	1627, 1932
AdeI	CACNNNGTG	1 cut(s)	406
AfaI	GTAC	2 cut(s)	1525, 2056
AfiI	CCNNNNNNNGG	4 cut(s)	333, 483, 767, 1270
AflII	CTTAAG	1 cut(s)	2015
AgsI	TTSAA	6 cut(s)	460, 586, 1295, 1666, 2140, 2186
AhlI	ACTAGT	1 cut(s)	677
AjiI	CACGTC	1 cut(s)	784
AjnI	CCWGG	1 cut(s)	1263
Alw21I	GWGCWC	7 cut(s)	117, 411, 1020, 1263, 1375, 1795, 2233
Alw26I	GTCTC	7 cut(s)	202, 774, 1454, 1822, 2129, 2149, 2657
Alw44I	GTGCAC	1 cut(s)	1259
AlwI	GGATC	4 cut(s)	263, 349, 940, 2620
AlwNI	CAGNNNCTG	1 cut(s)	1081
Aor13HI	TCCGGA	1 cut(s)	357
AoxI	GGCC	8 cut(s)	375, 552, 624, 630, 1342, 2295, 2476, 2596
ApaLI	GTGCAC	1 cut(s)	1259
ApeKI	GCWGC	3 cut(s)	1160, 2402, 2620
ApoI	RAATTY	2 cut(s)	1627, 1932
AseI	ATTAAT	5 cut(s)	957, 1124, 1758, 1859, 1991
AspLEI	GCGC	4 cut(s)	39, 423, 1877, 2022
AspS9I	GGNCC	4 cut(s)	474, 630, 2296, 2596
AsuC2I	CCSGG	1 cut(s)	628
AsuHPI	GGTGA	5 cut(s)	22, 37, 125, 1299, 1790
AvaII	GGWCC	1 cut(s)	474
AxyI	CCTNAGG	1 cut(s)	390
BaeGI	GKGCMC	1 cut(s)	1263
BalI	TGGCCA	1 cut(s)	1344
BanII	GRGCYC	1 cut(s)	117
BbsI	GAAGAC	2 cut(s)	63, 2177
Bbv12I	GWGCWC	7 cut(s)	117, 411, 1020, 1263, 1375, 1795, 2233
BbvCI	CCTCAGC	1 cut(s)	1611
BbvI	GCAGC	3 cut(s)	1147, 2414, 2607
BccI	CCATC	6 cut(s)	1177, 1439, 1544, 1964, 2206, 2573
BceAI	ACGGC	2 cut(s)	107, 451
BcgI	CGANNNNNNTGC	2 cut(s)	2436, 2470
BciT130I	CCWGG	1 cut(s)	1265
BclI	TGATCA	2 cut(s)	27, 2311
BcnI	CCSGG	1 cut(s)	628
BcoDI	GTCTC	7 cut(s)	202, 774, 1454, 1822, 2129, 2149, 2657
BcuI	ACTAGT	1 cut(s)	677
BfaI	CTAG	4 cut(s)	678, 947, 1059, 2411
BfmI	CTRYAG	2 cut(s)	1731, 1938
BfrI	CTTAAG	1 cut(s)	2015
BfuAI	ACCTGC	1 cut(s)	653
BisI	GCNGC	5 cut(s)	95, 345, 1161, 2403, 2621
BlsI	GCNGC	5 cut(s)	96, 346, 1162, 2404, 2622
Bme1390I	CCNGG	2 cut(s)	628, 1265
Bme18I	GGWCC	1 cut(s)	474
BmgBI	CACGTC	1 cut(s)	784
BmgT120I	GGNCC	4 cut(s)	474, 630, 2296, 2596
BmiI	GGNNCC	4 cut(s)	488, 631, 1413, 2597
BmrFI	CCNGG	2 cut(s)	628, 1265
BmsI	GCATC	8 cut(s)	331, 357, 385, 1068, 1521, 1570, 1697, 2373
BpiI	GAAGAC	2 cut(s)	63, 2177
BpmI	CTGGAG	1 cut(s)	1103
Bpu10I	CCTNAGC	2 cut(s)	1611, 2508
BpuEI	CTTGAG	4 cut(s)	356, 830, 1187, 1484
BpuMI	CCSGG	1 cut(s)	628
Bsa29I	ATCGAT	2 cut(s)	1443, 1575
BsaI	GGTCTC	3 cut(s)	1454, 1822, 2129
BsaJI	CCNNGG	4 cut(s)	591, 627, 1339, 2175
BsaWI	WCCGGW	2 cut(s)	135, 357
Bsc4I	CCNNNNNNNGG	4 cut(s)	333, 483, 767, 1270
Bse1I	ACTGG	2 cut(s)	488, 1345
Bse21I	CCTNAGG	1 cut(s)	390
Bse3DI	GCAATG	2 cut(s)	302, 1275
BseAI	TCCGGA	1 cut(s)	357
BseBI	CCWGG	1 cut(s)	1265
BseCI	ATCGAT	2 cut(s)	1443, 1575
BseDI	CCNNGG	4 cut(s)	591, 627, 1339, 2175
BseGI	GGATG	6 cut(s)	151, 400, 432, 730, 1688, 2656
BseLI	CCNNNNNNNGG	4 cut(s)	333, 483, 767, 1270
BseMI	GCAATG	2 cut(s)	302, 1275
BseMII	CTCAG	7 cut(s)	175, 381, 484, 1602, 1707, 2652, 2673
BseNI	ACTGG	2 cut(s)	488, 1345
BseRI	GAGGAG	5 cut(s)	38, 41, 44, 47, 299
BseSI	GKGCMC	1 cut(s)	1263
BseXI	GCAGC	3 cut(s)	1147, 2414, 2607
BsgI	GTGCAG	2 cut(s)	677, 1146
Bsh1236I	CGCG	2 cut(s)	37, 1877
BshFI	GGCC	8 cut(s)	377, 554, 626, 632, 1344, 2297, 2478, 2598
BshVI	ATCGAT	2 cut(s)	1443, 1575
BsiHKAI	GWGCWC	7 cut(s)	117, 411, 1020, 1263, 1375, 1795, 2233
BsiSI	CCGG	6 cut(s)	136, 358, 424, 569, 627, 761
BslFI	GGGAC	1 cut(s)	1721
BslI	CCNNNNNNNGG	4 cut(s)	333, 483, 767, 1270
BsmAI	GTCTC	7 cut(s)	202, 774, 1454, 1822, 2129, 2149, 2657
BsmBI	CGTCTC	1 cut(s)	774
BsmFI	GGGAC	1 cut(s)	1721
BsnI	GGCC	8 cut(s)	377, 554, 626, 632, 1344, 2297, 2478, 2598
Bso31I	GGTCTC	3 cut(s)	1454, 1822, 2129
Bsp1286I	GDGCHC	7 cut(s)	117, 411, 1020, 1263, 1375, 1795, 2233
Bsp13I	TCCGGA	1 cut(s)	357
Bsp143I	GATC	7 cut(s)	27, 255, 354, 932, 1786, 2311, 2612
Bsp19I	CCATGG	3 cut(s)	591, 1339, 2175
BspACI	CCGC	9 cut(s)	84, 92, 95, 214, 250, 345, 438, 566, 1898
BspANI	GGCC	8 cut(s)	377, 554, 626, 632, 1344, 2297, 2478, 2598
BspCNI	CTCAG	7 cut(s)	174, 382, 483, 1603, 1706, 2653, 2674
BspDI	ATCGAT	2 cut(s)	1443, 1575
BspEI	TCCGGA	1 cut(s)	357
BspFNI	CGCG	2 cut(s)	37, 1877
BspHI	TCATGA	1 cut(s)	2100
BspLI	GGNNCC	4 cut(s)	488, 631, 1413, 2597
BspMAI	CTGCAG	1 cut(s)	1942
BspMI	ACCTGC	1 cut(s)	653
BspPI	GGATC	4 cut(s)	263, 349, 940, 2620
BspQI	GCTCTTC	1 cut(s)	122
BspTI	CTTAAG	1 cut(s)	2015
BspTNI	GGTCTC	3 cut(s)	1454, 1822, 2129
BsrDI	GCAATG	2 cut(s)	302, 1275
BsrI	ACTGG	2 cut(s)	488, 1345
BssECI	CCNNGG	4 cut(s)	591, 627, 1339, 2175
BssMI	GATC	7 cut(s)	27, 255, 354, 932, 1786, 2311, 2612
BssT1I	CCWWGG	3 cut(s)	591, 1339, 2175
Bst2UI	CCWGG	1 cut(s)	1265
Bst4CI	ACNGT	9 cut(s)	22, 286, 535, 692, 910, 1153, 1433, 1735, 2629
Bst6I	CTCTTC	7 cut(s)	4, 112, 122, 205, 212, 1457, 2484
BstAFI	CTTAAG	1 cut(s)	2015
BstC8I	GCNNGC	8 cut(s)	214, 335, 379, 660, 1608, 2255, 2528, 2651
BstDEI	CTNAG	8 cut(s)	161, 390, 470, 1611, 1693, 2508, 2661, 2682
BstDSI	CCRYGG	3 cut(s)	591, 1339, 2175
BstF5I	GGATG	6 cut(s)	151, 400, 432, 730, 1688, 2656
BstFNI	CGCG	2 cut(s)	37, 1877
BstHHI	GCGC	4 cut(s)	39, 423, 1877, 2022
BstKTI	GATC	7 cut(s)	30, 258, 357, 935, 1789, 2314, 2615
BstMAI	GTCTC	7 cut(s)	202, 774, 1454, 1822, 2129, 2149, 2657
BstMBI	GATC	7 cut(s)	27, 255, 354, 932, 1786, 2311, 2612
BstMWI	GCNNNNNNNGC	3 cut(s)	1643, 1874, 2532
BstNI	CCWGG	1 cut(s)	1265
BstNSI	RCATGY	5 cut(s)	1033, 1291, 2026, 2390, 2530
BstSCI	CCNGG	2 cut(s)	626, 1263
BstSFI	CTRYAG	2 cut(s)	1731, 1938
BstSLI	GKGCMC	1 cut(s)	1263
BstUI	CGCG	2 cut(s)	37, 1877
BstV1I	GCAGC	3 cut(s)	1147, 2414, 2607
BstV2I	GAAGAC	2 cut(s)	63, 2177
BstX2I	RGATCY	1 cut(s)	354
BstYI	RGATCY	1 cut(s)	354
Bsu15I	ATCGAT	2 cut(s)	1443, 1575
Bsu36I	CCTNAGG	1 cut(s)	390
BsuRI	GGCC	8 cut(s)	377, 554, 626, 632, 1344, 2297, 2478, 2598
BsuTUI	ATCGAT	2 cut(s)	1443, 1575
BtgI	CCRYGG	3 cut(s)	591, 1339, 2175
BtgZI	GCGATG	1 cut(s)	812
BtrI	CACGTC	1 cut(s)	784
BtsCI	GGATG	6 cut(s)	151, 400, 432, 730, 1688, 2656
BtsI	GCAGTG	2 cut(s)	1274, 2124
BtsIMutI	CAGTG	5 cut(s)	1228, 1274, 1352, 2124, 2157
BveI	ACCTGC	1 cut(s)	653
Cac8I	GCNNGC	8 cut(s)	214, 335, 379, 660, 1608, 2255, 2528, 2651
CaiI	CAGNNNCTG	1 cut(s)	1081
CciI	TCATGA	1 cut(s)	2100
CfoI	GCGC	4 cut(s)	39, 423, 1877, 2022
Cfr13I	GGNCC	4 cut(s)	474, 630, 2296, 2596
ClaI	ATCGAT	2 cut(s)	1443, 1575
Csp6I	GTAC	2 cut(s)	1524, 2055
CspCI	CAANNNNNGTGG	2 cut(s)	765, 800
CviQI	GTAC	2 cut(s)	1524, 2055
DdeI	CTNAG	8 cut(s)	161, 390, 470, 1611, 1693, 2508, 2661, 2682
DpnI	GATC	7 cut(s)	29, 257, 356, 934, 1788, 2313, 2614
DpnII	GATC	7 cut(s)	27, 255, 354, 932, 1786, 2311, 2612
DraI	TTTAAA	2 cut(s)	967, 2604
DraIII	CACNNNGTG	1 cut(s)	406
EaeI	YGGCCR	3 cut(s)	552, 624, 1342
Eam1104I	CTCTTC	7 cut(s)	4, 112, 122, 205, 212, 1457, 2484
EarI	CTCTTC	7 cut(s)	4, 112, 122, 205, 212, 1457, 2484
EciI	GGCGGA	1 cut(s)	555
Ecl136II	GAGCTC	1 cut(s)	115
Eco130I	CCWWGG	3 cut(s)	591, 1339, 2175
Eco147I	AGGCCT	1 cut(s)	377
Eco24I	GRGCYC	1 cut(s)	117
Eco31I	GGTCTC	3 cut(s)	1454, 1822, 2129
Eco32I	GATATC	1 cut(s)	103
Eco47I	GGWCC	1 cut(s)	474
Eco53kI	GAGCTC	1 cut(s)	115
Eco81I	CCTNAGG	1 cut(s)	390
EcoICRI	GAGCTC	1 cut(s)	115
EcoO109I	RGGNCCY	1 cut(s)	474
EcoRII	CCWGG	1 cut(s)	1263
EcoRV	GATATC	1 cut(s)	103
EcoT14I	CCWWGG	3 cut(s)	591, 1339, 2175
EcoT22I	ATGCAT	3 cut(s)	925, 1123, 2388
EcoT38I	GRGCYC	1 cut(s)	117
ErhI	CCWWGG	3 cut(s)	591, 1339, 2175
Esp3I	CGTCTC	1 cut(s)	774
FaqI	GGGAC	1 cut(s)	1721
FauI	CCCGC	1 cut(s)	221
FauNDI	CATATG	2 cut(s)	1633, 2316
FbaI	TGATCA	2 cut(s)	27, 2311
Fnu4HI	GCNGC	5 cut(s)	95, 345, 1161, 2403, 2621
FokI	GGATG	6 cut(s)	138, 407, 439, 737, 1675, 2643
FriOI	GRGCYC	1 cut(s)	117
Fsp4HI	GCNGC	5 cut(s)	95, 345, 1161, 2403, 2621
FspBI	CTAG	4 cut(s)	678, 947, 1059, 2411
GlaI	GCGC	4 cut(s)	38, 422, 1876, 2021
GluI	GCNGC	5 cut(s)	95, 345, 1161, 2403, 2621
GsuI	CTGGAG	1 cut(s)	1103
HaeIII	GGCC	8 cut(s)	377, 554, 626, 632, 1344, 2297, 2478, 2598
HapII	CCGG	6 cut(s)	136, 358, 424, 569, 627, 761
HhaI	GCGC	4 cut(s)	39, 423, 1877, 2022
Hin6I	GCGC	4 cut(s)	37, 421, 1875, 2020
HinP1I	GCGC	4 cut(s)	37, 421, 1875, 2020
HincII	GTYRAC	2 cut(s)	1950, 2679
HindII	GTYRAC	2 cut(s)	1950, 2679
HindIII	AAGCTT	1 cut(s)	1046
HinfI	GANTC	8 cut(s)	383, 562, 1096, 1187, 1396, 1943, 2030, 2453
HpaII	CCGG	6 cut(s)	136, 358, 424, 569, 627, 761
HphI	GGTGA	5 cut(s)	22, 37, 125, 1299, 1790
Hpy166II	GTNNAC	7 cut(s)	289, 992, 1213, 1261, 1536, 1950, 2679
Hpy188I	TCNGA	5 cut(s)	112, 164, 1077, 1770, 2617
Hpy188III	TCNNGA	8 cut(s)	358, 936, 1415, 1501, 2101, 2126, 2148, 2660
Hpy8I	GTNNAC	7 cut(s)	289, 992, 1213, 1261, 1536, 1950, 2679
Hpy99I	CGWCG	2 cut(s)	437, 455
HpyAV	CCTTC	8 cut(s)	165, 198, 253, 933, 976, 1769, 1922, 2497
HpyCH4III	ACNGT	9 cut(s)	22, 286, 535, 692, 910, 1153, 1433, 1735, 2629
HpyCH4IV	ACGT	2 cut(s)	581, 783
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1643, 1874, 2532
HpyF3I	CTNAG	8 cut(s)	161, 390, 470, 1611, 1693, 2508, 2661, 2682
HpySE526I	ACGT	2 cut(s)	581, 783
HspAI	GCGC	4 cut(s)	37, 421, 1875, 2020
Kpn2I	TCCGGA	1 cut(s)	357
Ksp22I	TGATCA	2 cut(s)	27, 2311
Kzo9I	GATC	7 cut(s)	27, 255, 354, 932, 1786, 2311, 2612
LguI	GCTCTTC	1 cut(s)	122
LmnI	GCTCC	5 cut(s)	312, 492, 1417, 2197, 2236
Lsp1109I	GCAGC	3 cut(s)	1147, 2414, 2607
LweI	GCATC	8 cut(s)	331, 357, 385, 1068, 1521, 1570, 1697, 2373
MaeI	CTAG	4 cut(s)	678, 947, 1059, 2411
MaeII	ACGT	2 cut(s)	581, 783
MaeIII	GTNAC	6 cut(s)	577, 1108, 1318, 1553, 1778, 1826
MalI	GATC	7 cut(s)	29, 257, 356, 934, 1788, 2313, 2614
MboI	GATC	7 cut(s)	27, 255, 354, 932, 1786, 2311, 2612
MflI	RGATCY	1 cut(s)	354
MhlI	GDGCHC	7 cut(s)	117, 411, 1020, 1263, 1375, 1795, 2233
MlsI	TGGCCA	1 cut(s)	1344
MluNI	TGGCCA	1 cut(s)	1344
MlyI	GAGTC	3 cut(s)	1105, 1390, 2462
MmeI	TCCRAC	1 cut(s)	2197
Mox20I	TGGCCA	1 cut(s)	1344
Mph1103I	ATGCAT	3 cut(s)	925, 1123, 2388
MroI	TCCGGA	1 cut(s)	357
MscI	TGGCCA	1 cut(s)	1344
MslI	CAYNNNNRTG	2 cut(s)	2226, 2391
Msp20I	TGGCCA	1 cut(s)	1344
MspA1I	CMGCKG	1 cut(s)	252
MspCI	CTTAAG	1 cut(s)	2015
MspI	CCGG	6 cut(s)	136, 358, 424, 569, 627, 761
MspR9I	CCNGG	2 cut(s)	628, 1265
MvaI	CCWGG	1 cut(s)	1265
MvnI	CGCG	2 cut(s)	37, 1877
MwoI	GCNNNNNNNGC	3 cut(s)	1643, 1874, 2532
NciI	CCSGG	1 cut(s)	628
NcoI	CCATGG	3 cut(s)	591, 1339, 2175
NdeI	CATATG	2 cut(s)	1633, 2316
NdeII	GATC	7 cut(s)	27, 255, 354, 932, 1786, 2311, 2612
NlaIV	GGNNCC	4 cut(s)	488, 631, 1413, 2597
NmeAIII	GCCGAG	1 cut(s)	373
NmuCI	GTSAC	2 cut(s)	1108, 1778
NsiI	ATGCAT	3 cut(s)	925, 1123, 2388
NspI	RCATGY	5 cut(s)	1033, 1291, 2026, 2390, 2530
PacI	TTAATTAA	1 cut(s)	1758
PaeI	GCATGC	1 cut(s)	2530
PagI	TCATGA	1 cut(s)	2100
PceI	AGGCCT	1 cut(s)	377
PciSI	GCTCTTC	1 cut(s)	122
PcsI	WCGNNNNNNNCGW	1 cut(s)	345
PfeI	GAWTC	5 cut(s)	383, 562, 1187, 1943, 2030
PflMI	CCANNNNNTGG	2 cut(s)	333, 1270
PkrI	GCNGC	5 cut(s)	96, 346, 1162, 2404, 2622
PleI	GAGTC	3 cut(s)	1104, 1390, 2461
PpsI	GAGTC	3 cut(s)	1104, 1390, 2461
PpuMI	RGGWCCY	1 cut(s)	474
PshBI	ATTAAT	5 cut(s)	957, 1124, 1758, 1859, 1991
PsiI	TTATAA	2 cut(s)	1746, 2355
Psp124BI	GAGCTC	1 cut(s)	117
Psp5II	RGGWCCY	1 cut(s)	474
Psp6I	CCWGG	1 cut(s)	1263
PspGI	CCWGG	1 cut(s)	1263
PspN4I	GGNNCC	4 cut(s)	488, 631, 1413, 2597
PspPI	GGNCC	4 cut(s)	474, 630, 2296, 2596
PspPPI	RGGWCCY	1 cut(s)	474
PstI	CTGCAG	1 cut(s)	1942
PstNI	CAGNNNCTG	1 cut(s)	1081
PsuI	RGATCY	1 cut(s)	354
RsaI	GTAC	2 cut(s)	1525, 2056
RsaNI	GTAC	2 cut(s)	1524, 2055
RseI	CAYNNNNRTG	2 cut(s)	2226, 2391
SacI	GAGCTC	1 cut(s)	117
SapI	GCTCTTC	1 cut(s)	122
SatI	GCNGC	5 cut(s)	95, 345, 1161, 2403, 2621
Sau3AI	GATC	7 cut(s)	27, 255, 354, 932, 1786, 2311, 2612
Sau96I	GGNCC	4 cut(s)	474, 630, 2296, 2596
SchI	GAGTC	3 cut(s)	1105, 1390, 2462
ScrFI	CCNGG	2 cut(s)	628, 1265
SduI	GDGCHC	7 cut(s)	117, 411, 1020, 1263, 1375, 1795, 2233
SfaNI	GCATC	8 cut(s)	331, 357, 385, 1068, 1521, 1570, 1697, 2373
SfcI	CTRYAG	2 cut(s)	1731, 1938
SinI	GGWCC	1 cut(s)	474
SmiI	ATTTAAAT	1 cut(s)	2604
SmiMI	CAYNNNNRTG	2 cut(s)	2226, 2391
SmlI	CTYRAG	5 cut(s)	371, 845, 1166, 1499, 2015
SmoI	CTYRAG	5 cut(s)	371, 845, 1166, 1499, 2015
SpeI	ACTAGT	1 cut(s)	677
SphI	GCATGC	1 cut(s)	2530
SseBI	AGGCCT	1 cut(s)	377
SsiI	CCGC	9 cut(s)	84, 92, 95, 214, 250, 345, 438, 566, 1898
SspI	AATATT	1 cut(s)	1750
SspMI	CTAG	4 cut(s)	678, 947, 1059, 2411
SstI	GAGCTC	1 cut(s)	117
StuI	AGGCCT	1 cut(s)	377
StyD4I	CCNGG	2 cut(s)	626, 1263
StyI	CCWWGG	3 cut(s)	591, 1339, 2175
SwaI	ATTTAAAT	1 cut(s)	2604
TaaI	ACNGT	9 cut(s)	22, 286, 535, 692, 910, 1153, 1433, 1735, 2629
TaiI	ACGT	2 cut(s)	584, 786
TaqI	TCGA	8 cut(s)	339, 365, 411, 453, 1365, 1443, 1575, 2127
TatI	WGTACW	1 cut(s)	1523
TauI	GCSGC	2 cut(s)	97, 347
TfiI	GAWTC	5 cut(s)	383, 562, 1187, 1943, 2030
TscAI	CASTG	5 cut(s)	1228, 1281, 1352, 2124, 2164
TseFI	GTSAC	2 cut(s)	1108, 1778
TseI	GCWGC	3 cut(s)	1160, 2402, 2620
Tsp45I	GTSAC	2 cut(s)	1108, 1778
TspGWI	ACGGA	2 cut(s)	1312, 1970
TspRI	CASTG	5 cut(s)	1228, 1281, 1352, 2124, 2164
Van91I	CCANNNNNTGG	2 cut(s)	333, 1270
Vha464I	CTTAAG	1 cut(s)	2015
VneI	GTGCAC	1 cut(s)	1259
VpaK11BI	GGWCC	1 cut(s)	474
VspI	ATTAAT	5 cut(s)	957, 1124, 1758, 1859, 1991
XapI	RAATTY	2 cut(s)	1627, 1932
XceI	RCATGY	5 cut(s)	1033, 1291, 2026, 2390, 2530
XspI	CTAG	4 cut(s)	678, 947, 1059, 2411
Zsp2I	ATGCAT	3 cut(s)	925, 1123, 2388

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.