RchiOBHm_Chr2g0092171

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001743
Physical Location & Seq
Forward (+)
5680295 .. 5683089
2795 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46728

Sequence Viewer

Length: 2670 bp
ATGATCAGAAAAGCGACGGCCACGATCACCCCATTCAAGCACCAACTCTTGTTCTTCTTCTCCTCCTCCTCCTCTCCACCACCACCGGACAACCTCCCTTCCCAGCTCTTCGCCATCCTCTCCCGCCCAAACTGGCAAAAACACCCCTCCCTCAAAGCCCTTATCCCCTCCATATCCCCCTCCCACGTCTCCTCCCTCTTCTCCCTCAACCCGGTCCACCCCCGAACCGCCCTCCACTTCTTCACCTGGCTCGCCCACAACCCCAAATTCCACCACAACGTCCACTCCCACTCCTCCCTCCTCGCCCTCCTCCTCCGCAACGGCTTCTCCGGCCCCGCCGAGAGGGTCCGCATTTCGATGATTAAGGCTTCAATCTCTAACGCCGACGCCCGGTTCGTGCTGGACCGGTTGCGCCGGTTCAAGCACGAATTCGGGTTCAAGATCACCCTCAGGTGTTATGATTCTCTTTTATTTTCAATTTCCAAGTTTTTAGGGATTGAAGATTTGAAAATGTTGTATTTGGAAATGTTGGAGGATAAAATCTCCCCTGATTTGCATATATATAATATCATGGTGAATGCCTATTGTAGATTAGGCAATGTAGTTGAGGCTGGTGTCTATGTTAGTAAGATCGTTGAGGCGGGTTTGAAGCCGAATACGAATACTTACAATTCTTTGATAATGGGGCATTGTAGGAATAAGGATGTGGATAGTGCTTACAGGGCTTTTAAGGTGATCCCCAACAAGGGTTGTGCAAGAGATGTGGTTTCGTATAATGTATTGATACACGGGCTCTGTGAGGCTGGCAGGAGTAACGAGGCGTTTGAGTTGTACTCTAATATGGAGAAGGATGAGTGTTTACCAGATGCGCCGACGTATAATGTTCTTATTCATGCATTGTGTAGAGGGGGGAGGAGATTGGAAGGGATGAGTTTGTTTAAGGAGATGAAAGAGAAGGGTTGTGAGCCTAGTGTAAATACTTATAATGTGCTCATTACTAGCCTGTGTGACGAAAATAAGCTTCAGTATGCTAGGAAGGTTCTAGACCTGATGTCGGGGAAAGGGTTGGTTCCGGATGTTGTCACGTACAATGCATTGATTGATGGGTATTGCAAGGAGGGAGCGGTTGAGGCTGCGTTTGGGATTCTGGGTTTGATGGAATCTAATAAGTGTCGTCCAAATGCTCGCACGTTTACTGAATTGATTCATGGCTTTTGTAAAAGGAATAATGTGCACCAGGCAATGGCGCTTCTTGATAGGATGCTTGAACGGAAGCTCTCACCAGACCAGATTACATATAATTCGTTGATCAATGGGCAGTGTAAAGCAGGTCATGTAGATAGTGCTTATAGGTTGCTTAGTTTGATGAAACATAGTGGTTTAGTTCCTGACCAGTGGACTTACAGTGTCATCATCGACAGTCTCTGTAAGTTGGGGAGATTAAAAGAAGCTCAAGCCCTCTTCGATTCTCTCAAGGACAAAGGCATCAAATCTAATGAAGTGATATTTACGGCTTTACTTGATGGTTACTGCAAGATTGGGAAAATCGATGATGCTCGCACTTTATTTAATAGAATGCTTGCTGAGGGTTGTTTCCCAAACTCGTATACTTACTGTGCCTTCCTAGGTGGGCTGTGCAAAGAGGGAAAAACAAAGGAAGCATTATCAGTGGTGGAACAGATGCTAAGTAGTGGTGTGAAGCCTACAGTCCACACTTGTAATGTTCTAATTAACCATGTGCTGAAAGAAGGGGATTTCAACCATGCTCAGAGGTTTTTGAGTGAATTTGTTTCTCTTGGTTACCGACCTGATGCAGTCATGTACGGTACATTTGTTCATGCATATTGCAGCATAGATAATATAAAAGAAGCAGAGAAGGTAATGACTAAGATGGATGAAGAAGGAATTTCTGCAGATTCATTGACATACGCATTTTTAATCGAGGCATATCTTCGTTTGGGATTAATAGATTCTGCATTTGATGTCTTTAAGCGCATGTTTGATGCTCATTGCAACCCTTGTCATCGCATCTATTACTTGCTCATCAAACACCTGTCAAATGAAAAGTTGCTGCAGACCGACGATAATGTAGTAGGACTTCATATGGTTTCAAGTGTCCCATCAGCTGATATGTGGAAGACAGTGGATTTTGAAATTGCTTTAGAGCTCCTTGAGAAGATGAATGAACATGGTTGTGCACCGAATGTGAAAACTTACGGTGAGCTTATAGTAGCACTTTGCAAAGTTAGGAACTTGGAAGTAGCCCGTAAGTTGTATGCTCATATGAGAGATACCAGCATTTCTCCTAGTGAGGATATTTGTAACTCTCTTCTTAAATGTTGCTGCGAGTTGAAGGTGTATGGAGAAGCAGCTATTCTGGTGGACGCTATGATTCAGCTTGGTTATTTACCAGCATTGGAGTCGTGCAAATTGCTTGTATGTGGACTTTTTGAAGAAGAGAATAGTGAGAAGGCAAATTCTGTTTTCTGCAGAATTCTTTGTTGCGCGTATAACTACGATGAAGTGGCCTGGAAAGTTCTTCTTGAAGGTTTACTTACGAGGGGTCATATCAATAGATGCTCTGAGATGGTAACCGTCATCGAAAATATGGGTTACAAGCTTCATTCTGAGACCTATTCAATGTTAATTGAGGGATTTGATGGAATATAA

Protein Analysis

889

Amino Acids

99.95

Weight (kDa)

7.14

Isoelectric Point (pI)

31.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 173 - 280 1.8e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 183 - 232 5.2e-10 PPR repeat family
PPR_3 PF13812 209 - 263 5.7e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 215 - 244 4.6e-09 PPR repeat
PPR_2 PF13041 218 - 266 1.3e-13 PPR repeat family
PPR_long PF17177 232 - 374 5.8e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 248 - 299 5.2e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 249 - 282 3.9e-11 PPR repeat
PPR_2 PF13041 253 - 302 2.1e-18 PPR repeat family
PPR PF01535 256 - 286 8.1e-07 PPR repeat
PPR_3 PF13812 277 - 333 2.4e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 285 - 317 2.1e-07 PPR repeat
PPR_2 PF13041 292 - 337 4.2e-15 PPR repeat family
PPR PF01535 292 - 321 4.7e-08 PPR repeat
PPR_long PF17177 310 - 471 6e-14 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 319 - 351 6.1e-08 PPR repeat
PPR_2 PF13041 325 - 364 1.3e-08 PPR repeat family
TPR_24 PF23276 343 - 423 5.5e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 347 - 405 3e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 354 - 387 4.3e-12 PPR repeat
PPR_2 PF13041 358 - 407 1.5e-17 PPR repeat family
PPR PF01535 361 - 391 2.4e-07 PPR repeat
PPR_1 PF12854 391 - 421 1.1e-09 PPR repeat
PPR_3 PF13812 396 - 438 3.7e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 397 - 442 2.7e-13 PPR repeat family
PPR_3 PF13812 417 - 473 8.8e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 426 - 457 7.3e-12 PPR repeat
PPR_2 PF13041 428 - 477 5.7e-17 PPR repeat family
PPR PF01535 431 - 461 1.7e-06 PPR repeat
PPR_1 PF12854 460 - 490 7.6e-10 PPR repeat
PPR PF01535 467 - 496 3.4e-06 PPR repeat
PPR_long PF17177 482 - 584 9.8e-08 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 484 - 589 2.6e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 487 - 543 1.4e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 494 - 526 9.6e-09 PPR repeat
PPR_2 PF13041 499 - 547 3.2e-15 PPR repeat family
PPR PF01535 501 - 531 3.5e-07 PPR repeat
PPR_1 PF12854 530 - 561 7.9e-09 PPR repeat
PPR_2 PF13041 541 - 579 3.8e-09 PPR repeat family
PPR_3 PF13812 593 - 650 2.3e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 600 - 631 9.2e-06 PPR repeat
PPR_2 PF13041 603 - 649 3.6e-08 PPR repeat family
PPR_long PF17177 713 - 783 1.3e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 723 - 781 1.4e-07 Pentatricopeptide repeat domain
PPR_long PF17177 754 - 884 5.1e-06 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 984
Acc36I ACCTGC 1 cut(s) 1319
AccB7I CCANNNNNTGG 1 cut(s) 1243
AccBSI CCGCTC 1 cut(s) 1124
AccI GTMKAC 1 cut(s) 1607
AccII CGCG 1 cut(s) 2507
AccIII TCCGGA 1 cut(s) 1072
AciI CCGC 7 cut(s) 124, 228, 316, 336, 349, 641, 1124
AclWI GGATC 1 cut(s) 730
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 6 cut(s) 266, 428, 1784, 1905, 2476, 2493
AcuI CTGAAG 1 cut(s) 1007
AcyI GRCGYC 1 cut(s) 387
AfaI GTAC 4 cut(s) 833, 1088, 1823, 1828
AfiI CCNNNNNNNGG 7 cut(s) 211, 342, 390, 745, 746, 1054, 1243
AgeI ACCGGT 1 cut(s) 405
AhdI GACNNNNNGTC 1 cut(s) 1051
AjiI CACGTC 1 cut(s) 187
AjnI CCWGG 3 cut(s) 245, 1236, 2528
Alw21I GWGCWC 4 cut(s) 993, 1236, 2169, 2200
Alw26I GTCTC 3 cut(s) 193, 1427, 2624
Alw44I GTGCAC 2 cut(s) 1232, 2196
AlwI GGATC 1 cut(s) 730
AlwNI CAGNNNCTG 1 cut(s) 1425
Aor13HI TCCGGA 1 cut(s) 1072
AoxI GGCC 3 cut(s) 18, 331, 2526
ApaLI GTGCAC 2 cut(s) 1232, 2196
ApeKI GCWGC 5 cut(s) 1133, 1848, 2071, 2343, 2369
ApoI RAATTY 6 cut(s) 266, 428, 1784, 1905, 2476, 2493
ArsI GACNNNNNNTTYG 2 cut(s) 377, 409
AseI ATTAAT 1 cut(s) 1964
AsiGI ACCGGT 1 cut(s) 405
Asp700I GAANNNNTTC 1 cut(s) 1203
AspA2I CCTAGG 1 cut(s) 1624
AspLEI GCGC 5 cut(s) 414, 871, 1249, 1995, 2507
AspS9I GGNCC 4 cut(s) 214, 332, 346, 403
AsuC2I CCSGG 2 cut(s) 212, 391
AsuHPI GGTGA 7 cut(s) 19, 235, 436, 586, 745, 1272, 2231
AvaII GGWCC 3 cut(s) 214, 346, 403
AvrII CCTAGG 1 cut(s) 1624
AxyI CCTNAGG 1 cut(s) 449
BaeGI GKGCMC 2 cut(s) 1236, 2200
BaeI ACNNNNGTAYC 2 cut(s) 1818, 1851
BanII GRGCYC 2 cut(s) 795, 2169
BarI GAAGNNNNNNTAC 2 cut(s) 2082, 2114
BbsI GAAGAC 1 cut(s) 2144
Bbv12I GWGCWC 4 cut(s) 993, 1236, 2169, 2200
BbvCI CCTCAGC 1 cut(s) 1584
BbvI GCAGC 5 cut(s) 1120, 1860, 2058, 2330, 2381
BccI CCATC 8 cut(s) 122, 1097, 1150, 1517, 1885, 2128, 2581, 2654
BceAI ACGGC 3 cut(s) 33, 337, 1527
BcgI CGANNNNNNTGC 2 cut(s) 2403, 2437
BciT130I CCWGG 3 cut(s) 247, 1238, 2530
BclI TGATCA 2 cut(s) 3, 1308
BcnI CCSGG 2 cut(s) 212, 391
BcoDI GTCTC 3 cut(s) 193, 1427, 2624
BfaI CTAG 6 cut(s) 969, 999, 1032, 1043, 1625, 2307
BfmI CTRYAG 4 cut(s) 1704, 1911, 2072, 2488
BfoI RGCGCY 1 cut(s) 1250
BfuAI ACCTGC 1 cut(s) 1319
BisI GCNGC 5 cut(s) 1134, 1849, 2072, 2344, 2370
BlnI CCTAGG 1 cut(s) 1624
BlsI GCNGC 5 cut(s) 1135, 1850, 2073, 2345, 2371
Bme1390I CCNGG 5 cut(s) 212, 247, 391, 1238, 2530
Bme18I GGWCC 3 cut(s) 214, 346, 403
BmeRI GACNNNNNGTC 1 cut(s) 1051
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 4 cut(s) 214, 332, 346, 403
BmiI GGNNCC 3 cut(s) 334, 347, 1071
BmrFI CCNGG 5 cut(s) 212, 247, 391, 1238, 2530
BmsI GCATC 9 cut(s) 856, 1251, 1494, 1543, 1671, 1801, 1993, 2037, 2567
BpiI GAAGAC 1 cut(s) 2144
BplI GAGNNNNNCTC 2 cut(s) 818, 850
Bpu10I CCTNAGC 1 cut(s) 1584
BpuEI CTTGAG 3 cut(s) 1437, 1457, 2192
BpuMI CCSGG 2 cut(s) 212, 391
Bsa29I ATCGAT 1 cut(s) 1548
BsaAI YACGTR 1 cut(s) 1086
BsaHI GRCGYC 1 cut(s) 387
BsaI GGTCTC 1 cut(s) 2624
BsaJI CCNNGG 1 cut(s) 1624
BsaWI WCCGGW 3 cut(s) 85, 405, 1072
BsaXI ACNNNNNCTCC 8 cut(s) 176, 206, 269, 275, 299, 305, 311, 341
Bsc4I CCNNNNNNNGG 7 cut(s) 211, 342, 390, 745, 746, 1054, 1243
Bse118I RCCGGY 2 cut(s) 405, 414
Bse1I ACTGG 2 cut(s) 137, 1393
Bse21I CCTNAGG 1 cut(s) 449
Bse3DI GCAATG 3 cut(s) 604, 1248, 2008
BseAI TCCGGA 1 cut(s) 1072
BseBI CCWGG 3 cut(s) 247, 1238, 2530
BseCI ATCGAT 1 cut(s) 1548
BseDI CCNNGG 1 cut(s) 1624
BseGI GGATG 7 cut(s) 114, 709, 856, 933, 1081, 1266, 1900
BseLI CCNNNNNNNGG 7 cut(s) 211, 342, 390, 745, 746, 1054, 1243
BseMI GCAATG 3 cut(s) 604, 1248, 2008
BseMII CTCAG 5 cut(s) 463, 1575, 1781, 2574, 2619
BseNI ACTGG 2 cut(s) 137, 1393
BseSI GKGCMC 2 cut(s) 1236, 2200
BseXI GCAGC 5 cut(s) 1120, 1860, 2058, 2330, 2381
BseYI CCCAGC 1 cut(s) 102
Bsh1236I CGCG 1 cut(s) 2507
BshFI GGCC 3 cut(s) 20, 333, 2528
BshTI ACCGGT 1 cut(s) 405
BshVI ATCGAT 1 cut(s) 1548
BsiHKAI GWGCWC 4 cut(s) 993, 1236, 2169, 2200
BsiSI CCGG 7 cut(s) 86, 212, 330, 391, 406, 415, 1073
BslFI GGGAC 1 cut(s) 2102
BslI CCNNNNNNNGG 7 cut(s) 211, 342, 390, 745, 746, 1054, 1243
BsmAI GTCTC 3 cut(s) 193, 1427, 2624
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 2102
BsmI GAATGC 2 cut(s) 583, 1581
BsnI GGCC 3 cut(s) 20, 333, 2528
Bso31I GGTCTC 1 cut(s) 2624
Bsp1286I GDGCHC 5 cut(s) 795, 993, 1236, 2169, 2200
Bsp13I TCCGGA 1 cut(s) 1072
Bsp143I GATC 6 cut(s) 3, 24, 441, 630, 735, 1308
BspACI CCGC 7 cut(s) 124, 228, 316, 336, 349, 641, 1124
BspANI GGCC 3 cut(s) 20, 333, 2528
BspCNI CTCAG 5 cut(s) 462, 1576, 1780, 2575, 2620
BspDI ATCGAT 1 cut(s) 1548
BspEI TCCGGA 1 cut(s) 1072
BspFNI CGCG 1 cut(s) 2507
BspLI GGNNCC 3 cut(s) 334, 347, 1071
BspMAI CTGCAG 3 cut(s) 1915, 2076, 2492
BspMI ACCTGC 1 cut(s) 1319
BspPI GGATC 1 cut(s) 730
BspQI GCTCTTC 1 cut(s) 113
BspTNI GGTCTC 1 cut(s) 2624
BsrBI CCGCTC 1 cut(s) 1124
BsrDI GCAATG 3 cut(s) 604, 1248, 2008
BsrFI RCCGGY 2 cut(s) 405, 414
BsrI ACTGG 2 cut(s) 137, 1393
BssAI RCCGGY 2 cut(s) 405, 414
BssECI CCNNGG 1 cut(s) 1624
BssMI GATC 6 cut(s) 3, 24, 441, 630, 735, 1308
BssNAI GTATAC 1 cut(s) 1608
BssNI GRCGYC 1 cut(s) 387
BssT1I CCWWGG 1 cut(s) 1624
Bst1107I GTATAC 1 cut(s) 1608
Bst2UI CCWGG 3 cut(s) 247, 1238, 2530
Bst4CI ACNGT 8 cut(s) 1406, 1421, 1616, 1708, 1826, 2143, 2219, 2596
Bst6I CTCTTC 5 cut(s) 113, 203, 1466, 2334, 2451
BstACI GRCGYC 1 cut(s) 387
BstBAI YACGTR 1 cut(s) 1086
BstC8I GCNNGC 5 cut(s) 252, 805, 1186, 1558, 1581
BstDEI CTNAG 8 cut(s) 449, 1358, 1584, 1685, 1767, 1887, 2583, 2628
BstEII GGTNACC 2 cut(s) 1799, 2590
BstF5I GGATG 7 cut(s) 114, 709, 856, 933, 1081, 1266, 1900
BstFNI CGCG 1 cut(s) 2507
BstH2I RGCGCY 1 cut(s) 1250
BstHHI GCGC 5 cut(s) 414, 871, 1249, 1995, 2507
BstKTI GATC 6 cut(s) 6, 27, 444, 633, 738, 1311
BstMAI GTCTC 3 cut(s) 193, 1427, 2624
BstMBI GATC 6 cut(s) 3, 24, 441, 630, 735, 1308
BstMWI GCNNNNNNNGC 3 cut(s) 330, 722, 1130
BstNI CCWGG 3 cut(s) 247, 1238, 2530
BstNSI RCATGY 1 cut(s) 1999
BstPI GGTNACC 2 cut(s) 1799, 2590
BstSCI CCNGG 5 cut(s) 210, 245, 389, 1236, 2528
BstSFI CTRYAG 4 cut(s) 1704, 1911, 2072, 2488
BstSLI GKGCMC 2 cut(s) 1236, 2200
BstUI CGCG 1 cut(s) 2507
BstV1I GCAGC 5 cut(s) 1120, 1860, 2058, 2330, 2381
BstV2I GAAGAC 1 cut(s) 2144
BstZ17I GTATAC 1 cut(s) 1608
Bsu15I ATCGAT 1 cut(s) 1548
Bsu36I CCTNAGG 1 cut(s) 449
BsuRI GGCC 3 cut(s) 20, 333, 2528
BsuTUI ATCGAT 1 cut(s) 1548
BtgZI GCGATG 1 cut(s) 2009
BtrI CACGTC 1 cut(s) 187
BtsCI GGATG 7 cut(s) 114, 709, 856, 933, 1081, 1266, 1900
BtsI GCAGTG 1 cut(s) 1325
BtsIMutI CAGTG 5 cut(s) 1325, 1400, 1411, 1674, 2148
BveI ACCTGC 1 cut(s) 1319
Cac8I GCNNGC 5 cut(s) 252, 805, 1186, 1558, 1581
CaiI CAGNNNCTG 1 cut(s) 1425
CfoI GCGC 5 cut(s) 414, 871, 1249, 1995, 2507
Cfr10I RCCGGY 2 cut(s) 405, 414
Cfr13I GGNCC 4 cut(s) 214, 332, 346, 403
ClaI ATCGAT 1 cut(s) 1548
CseI GACGC 2 cut(s) 395, 2393
Csp6I GTAC 4 cut(s) 832, 1087, 1822, 1827
CspAI ACCGGT 1 cut(s) 405
CspCI CAANNNNNGTGG 2 cut(s) 744, 779
CviQI GTAC 4 cut(s) 832, 1087, 1822, 1827
DdeI CTNAG 8 cut(s) 449, 1358, 1584, 1685, 1767, 1887, 2583, 2628
DpnI GATC 6 cut(s) 5, 26, 443, 632, 737, 1310
DpnII GATC 6 cut(s) 3, 24, 441, 630, 735, 1308
DriI GACNNNNNGTC 1 cut(s) 1051
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 5 cut(s) 113, 203, 1466, 2334, 2451
Eam1105I GACNNNNNGTC 1 cut(s) 1051
EarI CTCTTC 5 cut(s) 113, 203, 1466, 2334, 2451
Ecl136II GAGCTC 1 cut(s) 2167
Eco130I CCWWGG 1 cut(s) 1624
Eco24I GRGCYC 2 cut(s) 795, 2169
Eco31I GGTCTC 1 cut(s) 2624
Eco47I GGWCC 3 cut(s) 214, 346, 403
Eco53kI GAGCTC 1 cut(s) 2167
Eco57I CTGAAG 1 cut(s) 1007
Eco81I CCTNAGG 1 cut(s) 449
Eco91I GGTNACC 2 cut(s) 1799, 2590
EcoICRI GAGCTC 1 cut(s) 2167
EcoO65I GGTNACC 2 cut(s) 1799, 2590
EcoRI GAATTC 2 cut(s) 428, 2493
EcoRII CCWGG 3 cut(s) 245, 1236, 2528
EcoT14I CCWWGG 1 cut(s) 1624
EcoT22I ATGCAT 3 cut(s) 898, 1096, 1843
EcoT38I GRGCYC 2 cut(s) 795, 2169
ErhI CCWWGG 1 cut(s) 1624
Esp3I CGTCTC 1 cut(s) 193
FalI AAGNNNNNCTT 4 cut(s) 2526, 2558, 2538, 2570
FaqI GGGAC 1 cut(s) 2102
FauI CCCGC 3 cut(s) 131, 343, 634
FauNDI CATATG 2 cut(s) 2103, 2283
FbaI TGATCA 2 cut(s) 3, 1308
FblI GTMKAC 1 cut(s) 1607
Fnu4HI GCNGC 5 cut(s) 1134, 1849, 2072, 2344, 2370
FokI GGATG 7 cut(s) 101, 716, 863, 940, 1088, 1273, 1907
FriOI GRGCYC 2 cut(s) 795, 2169
Fsp4HI GCNGC 5 cut(s) 1134, 1849, 2072, 2344, 2370
FspBI CTAG 6 cut(s) 969, 999, 1032, 1043, 1625, 2307
GlaI GCGC 5 cut(s) 413, 870, 1248, 1994, 2506
GluI GCNGC 5 cut(s) 1134, 1849, 2072, 2344, 2370
GsaI CCCAGC 1 cut(s) 106
HaeII RGCGCY 1 cut(s) 1250
HaeIII GGCC 3 cut(s) 20, 333, 2528
HapII CCGG 7 cut(s) 86, 212, 330, 391, 406, 415, 1073
HgaI GACGC 2 cut(s) 395, 2393
HhaI GCGC 5 cut(s) 414, 871, 1249, 1995, 2507
Hin1I GRCGYC 1 cut(s) 387
Hin6I GCGC 5 cut(s) 412, 869, 1247, 1993, 2505
HinP1I GCGC 5 cut(s) 412, 869, 1247, 1993, 2505
HindIII AAGCTT 2 cut(s) 1019, 2618
HinfI GANTC 9 cut(s) 461, 1144, 1160, 1204, 1466, 1916, 1970, 2392, 2420
HpaII CCGG 7 cut(s) 86, 212, 330, 391, 406, 415, 1073
HphI GGTGA 7 cut(s) 19, 235, 436, 586, 745, 1272, 2231
Hpy188I TCNGA 4 cut(s) 8, 1770, 2584, 2629
Hpy188III TCNNGA 6 cut(s) 439, 1043, 1073, 1253, 1388, 2543
Hpy99I CGWCG 4 cut(s) 19, 389, 877, 2084
HpyCH4III ACNGT 8 cut(s) 1406, 1421, 1616, 1708, 1826, 2143, 2219, 2596
HpyCH4IV ACGT 5 cut(s) 186, 279, 875, 1085, 1190
HpyF10VI GCNNNNNNNGC 3 cut(s) 330, 722, 1130
HpyF3I CTNAG 8 cut(s) 449, 1358, 1584, 1685, 1767, 1887, 2583, 2628
HpySE526I ACGT 5 cut(s) 186, 279, 875, 1085, 1190
Hsp92I GRCGYC 1 cut(s) 387
HspAI GCGC 5 cut(s) 412, 869, 1247, 1993, 2505
Kpn2I TCCGGA 1 cut(s) 1072
Ksp22I TGATCA 2 cut(s) 3, 1308
Kzo9I GATC 6 cut(s) 3, 24, 441, 630, 735, 1308
LguI GCTCTTC 1 cut(s) 113
LmnI GCTCC 2 cut(s) 1121, 2172
Lsp1109I GCAGC 5 cut(s) 1120, 1860, 2058, 2330, 2381
LweI GCATC 9 cut(s) 856, 1251, 1494, 1543, 1671, 1801, 1993, 2037, 2567
MaeI CTAG 6 cut(s) 969, 999, 1032, 1043, 1625, 2307
MaeII ACGT 5 cut(s) 186, 279, 875, 1085, 1190
MaeIII GTNAC 8 cut(s) 812, 1007, 1081, 1526, 1799, 2321, 2590, 2612
MalI GATC 6 cut(s) 5, 26, 443, 632, 737, 1310
MbiI CCGCTC 1 cut(s) 1124
MboI GATC 6 cut(s) 3, 24, 441, 630, 735, 1308
MhlI GDGCHC 5 cut(s) 795, 993, 1236, 2169, 2200
MlyI GAGTC 1 cut(s) 2429
MmeI TCCRAC 1 cut(s) 510
Mph1103I ATGCAT 3 cut(s) 898, 1096, 1843
MroI TCCGGA 1 cut(s) 1072
MroXI GAANNNNTTC 1 cut(s) 1203
MslI CAYNNNNRTG 2 cut(s) 356, 2193
MspA1I CMGCKG 1 cut(s) 2126
MspI CCGG 7 cut(s) 86, 212, 330, 391, 406, 415, 1073
MspR9I CCNGG 5 cut(s) 212, 247, 391, 1238, 2530
Mva1269I GAATGC 2 cut(s) 583, 1581
MvaI CCWGG 3 cut(s) 247, 1238, 2530
MvnI CGCG 1 cut(s) 2507
MwoI GCNNNNNNNGC 3 cut(s) 330, 722, 1130
NciI CCSGG 2 cut(s) 212, 391
NdeI CATATG 2 cut(s) 2103, 2283
NdeII GATC 6 cut(s) 3, 24, 441, 630, 735, 1308
NlaIV GGNNCC 3 cut(s) 334, 347, 1071
NmeAIII GCCGAG 1 cut(s) 364
NmuCI GTSAC 2 cut(s) 1007, 1081
NsiI ATGCAT 3 cut(s) 898, 1096, 1843
NspI RCATGY 1 cut(s) 1999
PciSI GCTCTTC 1 cut(s) 113
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PctI GAATGC 2 cut(s) 583, 1581
PdmI GAANNNNTTC 1 cut(s) 1203
PfeI GAWTC 8 cut(s) 461, 1144, 1160, 1204, 1466, 1916, 1970, 2392
PflMI CCANNNNNTGG 1 cut(s) 1243
PinAI ACCGGT 1 cut(s) 405
PkrI GCNGC 5 cut(s) 1135, 1850, 2073, 2345, 2371
PleI GAGTC 1 cut(s) 2428
PpsI GAGTC 1 cut(s) 2428
Ppu21I YACGTR 1 cut(s) 1086
PshBI ATTAAT 1 cut(s) 1964
PsiI TTATAA 1 cut(s) 984
Psp124BI GAGCTC 1 cut(s) 2169
Psp6I CCWGG 3 cut(s) 245, 1236, 2528
PspEI GGTNACC 2 cut(s) 1799, 2590
PspFI CCCAGC 1 cut(s) 102
PspGI CCWGG 3 cut(s) 245, 1236, 2528
PspN4I GGNNCC 3 cut(s) 334, 347, 1071
PspPI GGNCC 4 cut(s) 214, 332, 346, 403
PstI CTGCAG 3 cut(s) 1915, 2076, 2492
PstNI CAGNNNCTG 1 cut(s) 1425
PvuII CAGCTG 1 cut(s) 2126
RsaI GTAC 4 cut(s) 833, 1088, 1823, 1828
RsaNI GTAC 4 cut(s) 832, 1087, 1822, 1827
RseI CAYNNNNRTG 2 cut(s) 356, 2193
SacI GAGCTC 1 cut(s) 2169
SapI GCTCTTC 1 cut(s) 113
SatI GCNGC 5 cut(s) 1134, 1849, 2072, 2344, 2370
Sau3AI GATC 6 cut(s) 3, 24, 441, 630, 735, 1308
Sau96I GGNCC 4 cut(s) 214, 332, 346, 403
SchI GAGTC 1 cut(s) 2429
ScrFI CCNGG 5 cut(s) 212, 247, 391, 1238, 2530
SduI GDGCHC 5 cut(s) 795, 993, 1236, 2169, 2200
SfaNI GCATC 9 cut(s) 856, 1251, 1494, 1543, 1671, 1801, 1993, 2037, 2567
SfcI CTRYAG 4 cut(s) 1704, 1911, 2072, 2488
SinI GGWCC 3 cut(s) 214, 346, 403
SmiMI CAYNNNNRTG 2 cut(s) 356, 2193
SmlI CTYRAG 3 cut(s) 1452, 1472, 2171
SmoI CTYRAG 3 cut(s) 1452, 1472, 2171
SsiI CCGC 7 cut(s) 124, 228, 316, 336, 349, 641, 1124
SspMI CTAG 6 cut(s) 969, 999, 1032, 1043, 1625, 2307
SstI GAGCTC 1 cut(s) 2169
StyD4I CCNGG 5 cut(s) 210, 245, 389, 1236, 2528
StyI CCWWGG 1 cut(s) 1624
TaaI ACNGT 8 cut(s) 1406, 1421, 1616, 1708, 1826, 2143, 2219, 2596
TaiI ACGT 5 cut(s) 189, 282, 878, 1088, 1193
TaqI TCGA 6 cut(s) 356, 1416, 1464, 1548, 1941, 2601
TaqII GACCGA 1 cut(s) 2093
TatI WGTACW 1 cut(s) 831
TfiI GAWTC 8 cut(s) 461, 1144, 1160, 1204, 1466, 1916, 1970, 2392
TscAI CASTG 5 cut(s) 1325, 1400, 1411, 1674, 2148
TseFI GTSAC 2 cut(s) 1007, 1081
TseI GCWGC 5 cut(s) 1133, 1848, 2071, 2343, 2369
Tsp45I GTSAC 2 cut(s) 1007, 1081
TspGWI ACGGA 1 cut(s) 1285
TspRI CASTG 5 cut(s) 1325, 1400, 1411, 1674, 2148
Van91I CCANNNNNTGG 1 cut(s) 1243
VneI GTGCAC 2 cut(s) 1232, 2196
VpaK11BI GGWCC 3 cut(s) 214, 346, 403
VspI ATTAAT 1 cut(s) 1964
XapI RAATTY 6 cut(s) 266, 428, 1784, 1905, 2476, 2493
XbaI TCTAGA 1 cut(s) 1042
XceI RCATGY 1 cut(s) 1999
XmaJI CCTAGG 1 cut(s) 1624
XmiI GTMKAC 1 cut(s) 1607
XmnI GAANNNNTTC 1 cut(s) 1203
XspI CTAG 6 cut(s) 969, 999, 1032, 1043, 1625, 2307
Zsp2I ATGCAT 3 cut(s) 898, 1096, 1843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.