Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0001743

Physical Location & Seq

Genomic Coordinates Forward (+)

13467565 .. 13470190

Gene Structure 2626 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g12750.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2550 bp

ATGATGAGAAGAGAACTGACGGCGGTGATCACCACGCGCCAAACCCTCCTCCTCCTCCTCAAACCCTTATCTTCCCTCGCCACCGCAACAGCGCCGCCCGATATCTCTTCCGAGCTCTTCGCCGTCCTCTCCCAGCGGAATTGGCAGAAACACCCTTCCCTCAGAAACCTAATCCCCTCCATATCCCCTTCCCATGTCTCTTCCCTCTTCGCCCGCAACCTCCACCCCCAAATTGCCCTCTCCTTCTTCCGCTGGATCGCCGGAATCCCCCGATACAAGCACACCGTCCACTCCCATTCCTCTTTGCTCCTCAACATTCTGCTTCCCAATTGCTGGCTCGATGCCGCCGAGAAGATCCGGATTTCGATGCTCAAGGCCTGCGATTCGCCTGACGACGCCCGCTTTGTGCTCGACCTGTTGCGCCGGATGAACAACGGCGGTTCTGGTTTCGTCGAGTTCAAGCTCACTCTCAGGTCCTACAACTGGCTCCTAATATCTGTGTCTAAGTTTTTACTAATTGATGATGTGAAAACTGTGTATTTGGAAATGCTGGCCGACAAGATTCCGCCGGATATAGTTACGTTGAATACCATGGTGGTTGCTTATTGTAAATTGGGAAATGTGGCCGGGGCCAATTTGTATGTCAGTAAGATAGTGCAGGCAGGTTTTAGCCCCACTAGTTTTACATACAGTTCATTGATAATGGGGCATTGTAGGAACAAGGATGTGGATAATGCTTTTAAGATTTTTGAGGTAATGCCGGATAAGGGCTGTCCAAGAGACGTGGTTTCGTATAATTATTTAATTCGGGGGTTTTGTGAGGCTCATCGCATTGATGAGGCACTCAAGTTGTTCTCTCACTTGGGGCAGGGGGGCGATAATTGTTTTCCTAATGTGAGAACATATACAGTTCTTATTCATGCATTGTGTGGATCAAGAAGGAAACTAGAAGCAATTAATCTATTTAAAGAGATGATAGAGAAGGGTTGTGAACCTAATGTATATACTTATTGTGTGCTCATTGATAGCATGTGTAAGGAAAATAAGCTTGATTATGCTAGAAAGGTGCTAAATCAGATGCTGGAGAAAGGATTGAGTCCTAATGTCGTCACATACAATGCATTAATAAATGGGTATTGCAAGGAGGGAACAGTTGAGGCTGCACTTGAGATTTTGGCTGTGATGGAATCAAATGTTTGTTGTCCAGATGTTCACACATTCAGTGTATTGATTGACGGGTTTTGTAAAAGGAATAATGTGCACCAGGCAATGGCACTGCTTAACAAAATGCTTGAACGGAAGCTCTCACCAAACTTGGTTACATATAACTCATTACTCCGCGGCCAGTGTAAAGGAGGTCATTTCGATAGTGCTTATAGGTTGCTTAATTTGATGACTCATAGTGGTTTGGCTCCTGACCAGATTACTTACTGTATTTTCATCGACGGTCTCTGTAAGTGGAAGAGATTACAGGAAGCTCAAACCATGTTTGATACTCTCAAGAAGAATGGCATCAAGTCAAGTACAGAGATGTTTACTGCTTTAATTGATGGTTACTGCAAGATTGGGAAAATCGATGACGCCCTTTCTCTATTTGATACTATGCTTGTTGAGGATTGTTTGCCAAATTCATATGCTATCAATGCCTTGATACATGGGCTATTCAAAGAGAAAAAAATGATAGAAGCATCCTCAGTCATGGAAAAGATGTTAAGTATAGGTGTGAAGCCTACAGTCCCTCATTATAATATTTTAATTAATCATGTTCTGAAAGAAGGTGACTTTGATCGTGCTCATCAGCTTCTCAATAAAATGGTCTCTGTCGGTAACAAGCCTACTCTAATTACTTACAACACATTAATTCAGGCATATTGCGCGTTAGGGAATATAAATGAAGTGGAGAAGCTGATGAGTAAGATGAATGGAGAAGGAATTTCTGCAGATTCGTTGACTTACACATTTTTAATTGATGGATATGTCCGTATGGGATTAATTGATTGTGCGTTTGATGTTCTTAAGCGCATGTTTGATTCTGGTTGTGAGCCTTGTCATCGTACCTATTCCTTTCTAATCAAACATCTCTCAAATCAGAAGTTGGTCATGACAAACAGCAAAGCAGTGGGTCTCGATATAATGTTGAATGTCTCTGGAAATATCACTGATATATGGAAGACCATGGATTTTGAAATTGCTTTGGAGCTATTTGAGAAGATGGTTGGACATGGTTGTGCTCCCAATATGAACACCTATGGCGAGCTTATAGTAAGACTTTGCAAAGAGAGAAACTTAGAAGTGGCCCAGAGGTTATATGATCATATGAGAGATATGGGTATTTCTCCCAACGAAGATATTTATAATTCTCTTGTTAATTGTTGCTGTGAGATGCATGTGTATGGAGAGGCAGCAATTCTAGTGCACACTATGATTGAGCATGGTTATTTACCAGCATTGGAGTCGTGCAAGTTGCTTGTATGTGGCCTTTTTGATGAAGAGAATGATGAGAAGGCTAAGGAAGTTTTTTGTAGCATGCTTCAGTATCAGAGCTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

850

Amino Acids

95.62

Weight (kDa)

7.07

Isoelectric Point (pI)

33.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	190 - 239	2.4e-09	PPR repeat family
PPR_3	PF13812	217 - 270	4.1e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	222 - 253	2.7e-10	PPR repeat
PPR_2	PF13041	225 - 273	1.1e-14	PPR repeat family
PPR	PF01535	228 - 258	3.2e-07	PPR repeat
PPR_1	PF12854	256 - 286	2.8e-09	PPR repeat
PPR_2	PF13041	260 - 310	1.3e-13	PPR repeat family
PPR_1	PF12854	294 - 325	3.2e-07	PPR repeat
PPR_2	PF13041	297 - 346	3.6e-17	PPR repeat family
PPR_3	PF13812	297 - 343	2.8e-08	Pentatricopeptide repeat domain
PPR	PF01535	301 - 330	3.2e-06	PPR repeat
PPR_long	PF17177	317 - 472	1.9e-13	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	328 - 360	2.3e-11	PPR repeat
PPR_3	PF13812	334 - 380	7.4e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	340 - 378	2e-11	PPR repeat family
PPR_3	PF13812	355 - 414	2.5e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	363 - 396	1e-12	PPR repeat
PPR_2	PF13041	367 - 416	8.3e-19	PPR repeat family
PPR	PF01535	370 - 398	1.2e-06	PPR repeat
PPR_1	PF12854	400 - 430	2.7e-11	PPR repeat
PPR_3	PF13812	403 - 446	2.9e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	410 - 451	1.3e-12	PPR repeat family
PPR_1	PF12854	435 - 465	1.5e-08	PPR repeat
PPR_2	PF13041	437 - 486	4.2e-16	PPR repeat family
PPR_1	PF12854	469 - 499	9.3e-07	PPR repeat
PPR_1	PF12854	504 - 535	3.6e-08	PPR repeat
PPR_long	PF17177	509 - 690	4.3e-15	Pentacotripeptide-repeat region of PRORP
PPR	PF01535	511 - 540	4.3e-07	PPR repeat
PPR_2	PF13041	511 - 556	1.1e-11	PPR repeat family
PPR_2	PF13041	577 - 621	7.8e-11	PPR repeat family
PPR_1	PF12854	611 - 640	6.6e-07	PPR repeat
PPR_2	PF13041	612 - 660	1.1e-13	PPR repeat family
PPR	PF01535	615 - 645	1.1e-06	PPR repeat
PPR_3	PF13812	635 - 694	4.6e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	644 - 675	1.8e-06	PPR repeat
PPR_2	PF13041	648 - 695	2.3e-09	PPR repeat family
PPR_long	PF17177	717 - 841	4.6e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	734 - 793	1.6e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	753 - 793	9e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0001743)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77340 AT5G65560 AT5G65560 AT5G65560
fragaria_vesca	FvH4_1g06430 FvH4_2g11090 FvH4_2g11090 FvH4_2g11090 FvH4_2g11090 FvH4_2g11090 FvH4_7g04271 FvH4_7g04271
malus_domestica	MD03G1170600.v1.1 MD08G1245900.v1.1 MD11G1188000.v1.1 MD15G1441200.v1.1
prunus_persica	Prupe.1G583300_v2.0.a1 Prupe.4G256300_v2.0.a1
pyrus_communis	pycom03g12750 pycom08g21340 pycom15g38890 pycom15g38910
rosa_chinensis	RchiOBHm_Chr1g0329241 RchiOBHm_Chr2g0092171
rosa_laevigata	RLG00000016282 RLG00000029907
rosa_multiflora	Rmu_co8315275.1_g000001 Rmu_sc0000203.1_g000005 Rmu_sc0000524.1_g000014 Rmu_sc0010272.1_g000004 Rmu_sc0013552.1_g000009
rosa_roxburghii	Rroxscaffold_2G00149410 Rroxscaffold_4G00321300
rosa_rugosa	Rorug01G0077300 Rorug01G0077300 Rorug02G0023500
rosa_samantha	Rh1AG096400 Rh1BG077100 Rh1CG093100 Rh1CG107900 Rh1DG099700 Rh1DG116600 Rh2AG069100 Rh2BG068600 Rh2CG069700 Rh2DG067700
rosa_wichuraiana	Rw1G007460 Rw2G006310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1746, 2355
Acc36I	ACCTGC	1 cut(s)	653
AccB7I	CCANNNNNTGG	2 cut(s)	333, 1270
AccII	CGCG	3 cut(s)	37, 1341, 1877
AccIII	TCCGGA	1 cut(s)	357
AclWI	GGATC	3 cut(s)	263, 349, 940
AcoI	YGGCCR	3 cut(s)	552, 624, 1342
AcsI	RAATTY	2 cut(s)	1627, 1932
AcuI	CTGAAG	1 cut(s)	2516
AcyI	GRCGYC	2 cut(s)	396, 1581
AfaI	GTAC	2 cut(s)	1525, 2056
AfiI	CCNNNNNNNGG	4 cut(s)	333, 483, 767, 1270
AflII	CTTAAG	1 cut(s)	2015
AgsI	TTSAA	6 cut(s)	460, 586, 1295, 1666, 2140, 2186
AhlI	ACTAGT	1 cut(s)	677
AjiI	CACGTC	1 cut(s)	784
AjnI	CCWGG	1 cut(s)	1263
Alw21I	GWGCWC	7 cut(s)	117, 411, 1020, 1263, 1795, 2233, 2418
Alw26I	GTCTC	6 cut(s)	202, 774, 1454, 1822, 2129, 2149
Alw44I	GTGCAC	2 cut(s)	1259, 2414
AlwI	GGATC	3 cut(s)	263, 349, 940
AlwNI	CAGNNNCTG	1 cut(s)	1081
Aor13HI	TCCGGA	1 cut(s)	357
AoxI	GGCC	7 cut(s)	375, 552, 624, 630, 1342, 2295, 2476
ApaLI	GTGCAC	2 cut(s)	1259, 2414
ApeKI	GCWGC	3 cut(s)	1160, 2402, 2544
ApoI	RAATTY	2 cut(s)	1627, 1932
ArsI	GACNNNNNNTTYG	2 cut(s)	386, 418
AseI	ATTAAT	5 cut(s)	957, 1124, 1758, 1859, 1991
AspLEI	GCGC	5 cut(s)	39, 94, 423, 1877, 2022
AspS9I	GGNCC	3 cut(s)	474, 630, 2296
AsuC2I	CCSGG	1 cut(s)	628
AsuHPI	GGTGA	4 cut(s)	22, 37, 1299, 1790
AvaII	GGWCC	1 cut(s)	474
BaeGI	GKGCMC	2 cut(s)	1263, 2418
BanII	GRGCYC	1 cut(s)	117
BbsI	GAAGAC	1 cut(s)	2177
Bbv12I	GWGCWC	7 cut(s)	117, 411, 1020, 1263, 1795, 2233, 2418
BbvI	GCAGC	3 cut(s)	1147, 2414, 2531
BccI	CCATC	4 cut(s)	1177, 1544, 1964, 2206
BceAI	ACGGC	3 cut(s)	36, 107, 451
BcgI	CGANNNNNNTGC	2 cut(s)	2436, 2470
BciT130I	CCWGG	1 cut(s)	1265
BclI	TGATCA	2 cut(s)	27, 2311
BcnI	CCSGG	1 cut(s)	628
BcoDI	GTCTC	6 cut(s)	202, 774, 1454, 1822, 2129, 2149
BcuI	ACTAGT	1 cut(s)	677
BfaI	CTAG	4 cut(s)	678, 947, 1059, 2411
BfmI	CTRYAG	2 cut(s)	1731, 1938
BfoI	RGCGCY	1 cut(s)	95
BfrI	CTTAAG	1 cut(s)	2015
BfuAI	ACCTGC	1 cut(s)	653
BisI	GCNGC	6 cut(s)	95, 345, 1161, 1342, 2403, 2545
BlsI	GCNGC	6 cut(s)	96, 346, 1162, 1343, 2404, 2546
Bme1390I	CCNGG	2 cut(s)	628, 1265
Bme18I	GGWCC	1 cut(s)	474
BmgBI	CACGTC	1 cut(s)	784
BmgT120I	GGNCC	3 cut(s)	474, 630, 2296
BmiI	GGNNCC	3 cut(s)	488, 631, 1413
BmrFI	CCNGG	2 cut(s)	628, 1265
BmsI	GCATC	6 cut(s)	331, 357, 1068, 1521, 1697, 2373
BpiI	GAAGAC	1 cut(s)	2177
BpmI	CTGGAG	1 cut(s)	1103
Bpu10I	CCTNAGC	1 cut(s)	2508
BpuEI	CTTGAG	4 cut(s)	356, 830, 1187, 1484
BpuMI	CCSGG	1 cut(s)	628
Bsa29I	ATCGAT	1 cut(s)	1575
BsaHI	GRCGYC	2 cut(s)	396, 1581
BsaI	GGTCTC	3 cut(s)	1454, 1822, 2129
BsaJI	CCNNGG	4 cut(s)	591, 627, 1339, 2175
BsaWI	WCCGGW	1 cut(s)	357
Bsc4I	CCNNNNNNNGG	4 cut(s)	333, 483, 767, 1270
Bse1I	ACTGG	2 cut(s)	488, 1345
Bse3DI	GCAATG	1 cut(s)	1275
BseAI	TCCGGA	1 cut(s)	357
BseBI	CCWGG	1 cut(s)	1265
BseCI	ATCGAT	1 cut(s)	1575
BseDI	CCNNGG	4 cut(s)	591, 627, 1339, 2175
BseGI	GGATG	3 cut(s)	432, 730, 1688
BseLI	CCNNNNNNNGG	4 cut(s)	333, 483, 767, 1270
BseMI	GCAATG	1 cut(s)	1275
BseMII	CTCAG	3 cut(s)	175, 484, 1707
BseNI	ACTGG	2 cut(s)	488, 1345
BseRI	GAGGAG	5 cut(s)	38, 41, 44, 47, 299
BseSI	GKGCMC	2 cut(s)	1263, 2418
BseXI	GCAGC	3 cut(s)	1147, 2414, 2531
BseYI	CCCAGC	1 cut(s)	132
BsgI	GTGCAG	2 cut(s)	677, 1146
Bsh1236I	CGCG	3 cut(s)	37, 1341, 1877
BshFI	GGCC	7 cut(s)	377, 554, 626, 632, 1344, 2297, 2478
BshVI	ATCGAT	1 cut(s)	1575
BsiHKAI	GWGCWC	7 cut(s)	117, 411, 1020, 1263, 1795, 2233, 2418
BsiSI	CCGG	6 cut(s)	261, 358, 424, 569, 627, 761
BslFI	GGGAC	1 cut(s)	1721
BslI	CCNNNNNNNGG	4 cut(s)	333, 483, 767, 1270
BsmAI	GTCTC	6 cut(s)	202, 774, 1454, 1822, 2129, 2149
BsmBI	CGTCTC	1 cut(s)	774
BsmFI	GGGAC	1 cut(s)	1721
BsnI	GGCC	7 cut(s)	377, 554, 626, 632, 1344, 2297, 2478
Bso31I	GGTCTC	3 cut(s)	1454, 1822, 2129
Bsp1286I	GDGCHC	7 cut(s)	117, 411, 1020, 1263, 1795, 2233, 2418
Bsp13I	TCCGGA	1 cut(s)	357
Bsp143I	GATC	6 cut(s)	27, 255, 354, 932, 1786, 2311
Bsp19I	CCATGG	2 cut(s)	591, 2175
BspANI	GGCC	7 cut(s)	377, 554, 626, 632, 1344, 2297, 2478
BspCNI	CTCAG	3 cut(s)	174, 483, 1706
BspDI	ATCGAT	1 cut(s)	1575
BspEI	TCCGGA	1 cut(s)	357
BspFNI	CGCG	3 cut(s)	37, 1341, 1877
BspHI	TCATGA	1 cut(s)	2100
BspLI	GGNNCC	3 cut(s)	488, 631, 1413
BspMAI	CTGCAG	1 cut(s)	1942
BspMI	ACCTGC	1 cut(s)	653
BspPI	GGATC	3 cut(s)	263, 349, 940
BspQI	GCTCTTC	1 cut(s)	122
BspTI	CTTAAG	1 cut(s)	2015
BspTNI	GGTCTC	3 cut(s)	1454, 1822, 2129
BsrDI	GCAATG	1 cut(s)	1275
BsrI	ACTGG	2 cut(s)	488, 1345
BssECI	CCNNGG	4 cut(s)	591, 627, 1339, 2175
BssMI	GATC	6 cut(s)	27, 255, 354, 932, 1786, 2311
BssNI	GRCGYC	2 cut(s)	396, 1581
BssT1I	CCWWGG	2 cut(s)	591, 2175
Bst2UI	CCWGG	1 cut(s)	1265
Bst4CI	ACNGT	8 cut(s)	286, 535, 692, 910, 1153, 1433, 1448, 1735
Bst6I	CTCTTC	7 cut(s)	4, 112, 122, 205, 212, 1457, 2484
BstACI	GRCGYC	2 cut(s)	396, 1581
BstAFI	CTTAAG	1 cut(s)	2015
BstC8I	GCNNGC	8 cut(s)	214, 335, 379, 400, 552, 660, 2255, 2528
BstDEI	CTNAG	6 cut(s)	161, 470, 504, 1693, 2287, 2508
BstDSI	CCRYGG	3 cut(s)	591, 1339, 2175
BstF5I	GGATG	3 cut(s)	432, 730, 1688
BstFNI	CGCG	3 cut(s)	37, 1341, 1877
BstH2I	RGCGCY	1 cut(s)	95
BstHHI	GCGC	5 cut(s)	39, 94, 423, 1877, 2022
BstKTI	GATC	6 cut(s)	30, 258, 357, 935, 1789, 2314
BstMAI	GTCTC	6 cut(s)	202, 774, 1454, 1822, 2129, 2149
BstMBI	GATC	6 cut(s)	27, 255, 354, 932, 1786, 2311
BstMWI	GCNNNNNNNGC	3 cut(s)	142, 1643, 1874
BstNI	CCWGG	1 cut(s)	1265
BstNSI	RCATGY	4 cut(s)	1033, 2026, 2390, 2530
BstSCI	CCNGG	2 cut(s)	626, 1263
BstSFI	CTRYAG	2 cut(s)	1731, 1938
BstSLI	GKGCMC	2 cut(s)	1263, 2418
BstUI	CGCG	3 cut(s)	37, 1341, 1877
BstV1I	GCAGC	3 cut(s)	1147, 2414, 2531
BstV2I	GAAGAC	1 cut(s)	2177
BstX2I	RGATCY	1 cut(s)	354
BstYI	RGATCY	1 cut(s)	354
Bsu15I	ATCGAT	1 cut(s)	1575
BsuRI	GGCC	7 cut(s)	377, 554, 626, 632, 1344, 2297, 2478
BsuTUI	ATCGAT	1 cut(s)	1575
BtgI	CCRYGG	3 cut(s)	591, 1339, 2175
BtgZI	GCGATG	1 cut(s)	812
BtrI	CACGTC	1 cut(s)	784
BtsCI	GGATG	3 cut(s)	432, 730, 1688
BtsI	GCAGTG	2 cut(s)	1274, 2124
BtsIMutI	CAGTG	5 cut(s)	1228, 1274, 1352, 2124, 2157
BveI	ACCTGC	1 cut(s)	653
Cac8I	GCNNGC	8 cut(s)	214, 335, 379, 400, 552, 660, 2255, 2528
CaiI	CAGNNNCTG	1 cut(s)	1081
CciI	TCATGA	1 cut(s)	2100
CfoI	GCGC	5 cut(s)	39, 94, 423, 1877, 2022
Cfr13I	GGNCC	3 cut(s)	474, 630, 2296
Cfr42I	CCGCGG	1 cut(s)	1342
ClaI	ATCGAT	1 cut(s)	1575
CseI	GACGC	2 cut(s)	404, 1589
Csp6I	GTAC	2 cut(s)	1524, 2055
CspCI	CAANNNNNGTGG	2 cut(s)	765, 800
CviQI	GTAC	2 cut(s)	1524, 2055
DdeI	CTNAG	6 cut(s)	161, 470, 504, 1693, 2287, 2508
DpnI	GATC	6 cut(s)	29, 257, 356, 934, 1788, 2313
DpnII	GATC	6 cut(s)	27, 255, 354, 932, 1786, 2311
DraI	TTTAAA	1 cut(s)	967
EaeI	YGGCCR	3 cut(s)	552, 624, 1342
Eam1104I	CTCTTC	7 cut(s)	4, 112, 122, 205, 212, 1457, 2484
EarI	CTCTTC	7 cut(s)	4, 112, 122, 205, 212, 1457, 2484
EciI	GGCGGA	1 cut(s)	555
Ecl136II	GAGCTC	1 cut(s)	115
Eco130I	CCWWGG	2 cut(s)	591, 2175
Eco147I	AGGCCT	1 cut(s)	377
Eco24I	GRGCYC	1 cut(s)	117
Eco31I	GGTCTC	3 cut(s)	1454, 1822, 2129
Eco32I	GATATC	1 cut(s)	103
Eco47I	GGWCC	1 cut(s)	474
Eco53kI	GAGCTC	1 cut(s)	115
Eco57I	CTGAAG	1 cut(s)	2516
EcoICRI	GAGCTC	1 cut(s)	115
EcoO109I	RGGNCCY	1 cut(s)	474
EcoRII	CCWGG	1 cut(s)	1263
EcoRV	GATATC	1 cut(s)	103
EcoT14I	CCWWGG	2 cut(s)	591, 2175
EcoT22I	ATGCAT	3 cut(s)	925, 1123, 2388
EcoT38I	GRGCYC	1 cut(s)	117
ErhI	CCWWGG	2 cut(s)	591, 2175
Esp3I	CGTCTC	1 cut(s)	774
FaqI	GGGAC	1 cut(s)	1721
FauI	CCCGC	2 cut(s)	221, 407
FauNDI	CATATG	2 cut(s)	1633, 2316
FbaI	TGATCA	2 cut(s)	27, 2311
Fnu4HI	GCNGC	6 cut(s)	95, 345, 1161, 1342, 2403, 2545
FokI	GGATG	3 cut(s)	439, 737, 1675
FriOI	GRGCYC	1 cut(s)	117
Fsp4HI	GCNGC	6 cut(s)	95, 345, 1161, 1342, 2403, 2545
FspBI	CTAG	4 cut(s)	678, 947, 1059, 2411
GlaI	GCGC	5 cut(s)	38, 93, 422, 1876, 2021
GluI	GCNGC	6 cut(s)	95, 345, 1161, 1342, 2403, 2545
GsaI	CCCAGC	1 cut(s)	136
GsuI	CTGGAG	1 cut(s)	1103
HaeII	RGCGCY	1 cut(s)	95
HaeIII	GGCC	7 cut(s)	377, 554, 626, 632, 1344, 2297, 2478
HapII	CCGG	6 cut(s)	261, 358, 424, 569, 627, 761
HgaI	GACGC	2 cut(s)	404, 1589
HhaI	GCGC	5 cut(s)	39, 94, 423, 1877, 2022
Hin1I	GRCGYC	2 cut(s)	396, 1581
Hin6I	GCGC	5 cut(s)	37, 92, 421, 1875, 2020
HinP1I	GCGC	5 cut(s)	37, 92, 421, 1875, 2020
HincII	GTYRAC	1 cut(s)	1950
HindII	GTYRAC	1 cut(s)	1950
HindIII	AAGCTT	1 cut(s)	1046
HinfI	GANTC	9 cut(s)	264, 383, 562, 1096, 1187, 1396, 1943, 2030, 2453
HpaII	CCGG	6 cut(s)	261, 358, 424, 569, 627, 761
HphI	GGTGA	4 cut(s)	22, 37, 1299, 1790
Hpy166II	GTNNAC	7 cut(s)	289, 992, 1213, 1261, 1536, 1950, 2416
Hpy188I	TCNGA	6 cut(s)	112, 164, 1077, 1770, 2091, 2541
Hpy188III	TCNNGA	8 cut(s)	358, 936, 1205, 1415, 1501, 2101, 2126, 2148
Hpy8I	GTNNAC	7 cut(s)	289, 992, 1213, 1261, 1536, 1950, 2416
Hpy99I	CGWCG	3 cut(s)	398, 455, 1448
HpyAV	CCTTC	8 cut(s)	165, 198, 253, 933, 976, 1769, 1922, 2497
HpyCH4III	ACNGT	8 cut(s)	286, 535, 692, 910, 1153, 1433, 1448, 1735
HpyCH4IV	ACGT	2 cut(s)	581, 783
HpyF10VI	GCNNNNNNNGC	3 cut(s)	142, 1643, 1874
HpyF3I	CTNAG	6 cut(s)	161, 470, 504, 1693, 2287, 2508
HpySE526I	ACGT	2 cut(s)	581, 783
Hsp92I	GRCGYC	2 cut(s)	396, 1581
HspAI	GCGC	5 cut(s)	37, 92, 421, 1875, 2020
Kpn2I	TCCGGA	1 cut(s)	357
Ksp22I	TGATCA	2 cut(s)	27, 2311
KspI	CCGCGG	1 cut(s)	1342
Kzo9I	GATC	6 cut(s)	27, 255, 354, 932, 1786, 2311
LguI	GCTCTTC	1 cut(s)	122
LmnI	GCTCC	5 cut(s)	312, 492, 1417, 2197, 2236
Lsp1109I	GCAGC	3 cut(s)	1147, 2414, 2531
LweI	GCATC	6 cut(s)	331, 357, 1068, 1521, 1697, 2373
MaeI	CTAG	4 cut(s)	678, 947, 1059, 2411
MaeII	ACGT	2 cut(s)	581, 783
MaeIII	GTNAC	6 cut(s)	577, 1108, 1318, 1553, 1778, 1826
MalI	GATC	6 cut(s)	29, 257, 356, 934, 1788, 2313
MboI	GATC	6 cut(s)	27, 255, 354, 932, 1786, 2311
MfeI	CAATTG	1 cut(s)	328
MflI	RGATCY	1 cut(s)	354
MhlI	GDGCHC	7 cut(s)	117, 411, 1020, 1263, 1795, 2233, 2418
MlyI	GAGTC	3 cut(s)	1105, 1390, 2462
MmeI	TCCRAC	1 cut(s)	2197
Mph1103I	ATGCAT	3 cut(s)	925, 1123, 2388
MroI	TCCGGA	1 cut(s)	357
MslI	CAYNNNNRTG	2 cut(s)	2226, 2391
MspA1I	CMGCKG	3 cut(s)	136, 252, 1341
MspCI	CTTAAG	1 cut(s)	2015
MspI	CCGG	6 cut(s)	261, 358, 424, 569, 627, 761
MspR9I	CCNGG	2 cut(s)	628, 1265
MunI	CAATTG	1 cut(s)	328
MvaI	CCWGG	1 cut(s)	1265
MvnI	CGCG	3 cut(s)	37, 1341, 1877
MwoI	GCNNNNNNNGC	3 cut(s)	142, 1643, 1874
NciI	CCSGG	1 cut(s)	628
NcoI	CCATGG	2 cut(s)	591, 2175
NdeI	CATATG	2 cut(s)	1633, 2316
NdeII	GATC	6 cut(s)	27, 255, 354, 932, 1786, 2311
NlaIV	GGNNCC	3 cut(s)	488, 631, 1413
NmeAIII	GCCGAG	1 cut(s)	373
NmuCI	GTSAC	2 cut(s)	1108, 1778
NsiI	ATGCAT	3 cut(s)	925, 1123, 2388
NspI	RCATGY	4 cut(s)	1033, 2026, 2390, 2530
PacI	TTAATTAA	1 cut(s)	1758
PaeI	GCATGC	1 cut(s)	2530
PagI	TCATGA	1 cut(s)	2100
PceI	AGGCCT	1 cut(s)	377
PciSI	GCTCTTC	1 cut(s)	122
PcsI	WCGNNNNNNNCGW	1 cut(s)	345
PfeI	GAWTC	6 cut(s)	264, 383, 562, 1187, 1943, 2030
PflMI	CCANNNNNTGG	2 cut(s)	333, 1270
PkrI	GCNGC	6 cut(s)	96, 346, 1162, 1343, 2404, 2546
PleI	GAGTC	3 cut(s)	1104, 1390, 2461
PpsI	GAGTC	3 cut(s)	1104, 1390, 2461
PpuMI	RGGWCCY	1 cut(s)	474
PshBI	ATTAAT	5 cut(s)	957, 1124, 1758, 1859, 1991
PsiI	TTATAA	2 cut(s)	1746, 2355
Psp124BI	GAGCTC	1 cut(s)	117
Psp5II	RGGWCCY	1 cut(s)	474
Psp6I	CCWGG	1 cut(s)	1263
PspFI	CCCAGC	1 cut(s)	132
PspGI	CCWGG	1 cut(s)	1263
PspN4I	GGNNCC	3 cut(s)	488, 631, 1413
PspPI	GGNCC	3 cut(s)	474, 630, 2296
PspPPI	RGGWCCY	1 cut(s)	474
PstI	CTGCAG	1 cut(s)	1942
PstNI	CAGNNNCTG	1 cut(s)	1081
PsuI	RGATCY	1 cut(s)	354
RsaI	GTAC	2 cut(s)	1525, 2056
RsaNI	GTAC	2 cut(s)	1524, 2055
RseI	CAYNNNNRTG	2 cut(s)	2226, 2391
SacI	GAGCTC	1 cut(s)	117
SacII	CCGCGG	1 cut(s)	1342
SapI	GCTCTTC	1 cut(s)	122
SatI	GCNGC	6 cut(s)	95, 345, 1161, 1342, 2403, 2545
Sau3AI	GATC	6 cut(s)	27, 255, 354, 932, 1786, 2311
Sau96I	GGNCC	3 cut(s)	474, 630, 2296
SchI	GAGTC	3 cut(s)	1105, 1390, 2462
ScrFI	CCNGG	2 cut(s)	628, 1265
SduI	GDGCHC	7 cut(s)	117, 411, 1020, 1263, 1795, 2233, 2418
SfaNI	GCATC	6 cut(s)	331, 357, 1068, 1521, 1697, 2373
SfcI	CTRYAG	2 cut(s)	1731, 1938
Sfr303I	CCGCGG	1 cut(s)	1342
SgrBI	CCGCGG	1 cut(s)	1342
SinI	GGWCC	1 cut(s)	474
SmiMI	CAYNNNNRTG	2 cut(s)	2226, 2391
SmlI	CTYRAG	5 cut(s)	371, 845, 1166, 1499, 2015
SmoI	CTYRAG	5 cut(s)	371, 845, 1166, 1499, 2015
SpeI	ACTAGT	1 cut(s)	677
SphI	GCATGC	1 cut(s)	2530
SseBI	AGGCCT	1 cut(s)	377
SspI	AATATT	1 cut(s)	1750
SspMI	CTAG	4 cut(s)	678, 947, 1059, 2411
SstI	GAGCTC	1 cut(s)	117
StuI	AGGCCT	1 cut(s)	377
StyD4I	CCNGG	2 cut(s)	626, 1263
StyI	CCWWGG	2 cut(s)	591, 2175
TaaI	ACNGT	8 cut(s)	286, 535, 692, 910, 1153, 1433, 1448, 1735
TaiI	ACGT	2 cut(s)	584, 786
TaqI	TCGA	8 cut(s)	339, 365, 411, 453, 1365, 1443, 1575, 2127
TatI	WGTACW	1 cut(s)	1523
TauI	GCSGC	3 cut(s)	97, 347, 1344
TfiI	GAWTC	6 cut(s)	264, 383, 562, 1187, 1943, 2030
TscAI	CASTG	5 cut(s)	1228, 1281, 1352, 2124, 2164
TseFI	GTSAC	2 cut(s)	1108, 1778
TseI	GCWGC	3 cut(s)	1160, 2402, 2544
Tsp45I	GTSAC	2 cut(s)	1108, 1778
TspDTI	ATGAA	9 cut(s)	443, 684, 908, 1429, 1620, 1908, 1934, 2255, 2502
TspGWI	ACGGA	2 cut(s)	1312, 1970
TspRI	CASTG	5 cut(s)	1228, 1281, 1352, 2124, 2164
Van91I	CCANNNNNTGG	2 cut(s)	333, 1270
Vha464I	CTTAAG	1 cut(s)	2015
VneI	GTGCAC	2 cut(s)	1259, 2414
VpaK11BI	GGWCC	1 cut(s)	474
VspI	ATTAAT	5 cut(s)	957, 1124, 1758, 1859, 1991
XapI	RAATTY	2 cut(s)	1627, 1932
XceI	RCATGY	4 cut(s)	1033, 2026, 2390, 2530
XspI	CTAG	4 cut(s)	678, 947, 1059, 2411
Zsp2I	ATGCAT	3 cut(s)	925, 1123, 2388

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.