Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0009543

Physical Location & Seq

Genomic Coordinates Forward (+)

3630404 .. 3632606

Gene Structure 2203 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g05430.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 861 bp

ATGAAGATCGAGGAGTTGGAGGCGGAGGGGGATTGCGGCGTTCGGAACGACGCCGTTTCCGGGCGGCAGGTAGTTAGGGTTGACGAGGCTAAGAAAGCGCTTGTTGGAGCCGGGGCGCGGATCCTTTTTTACCCGACCCTGTTGTATAATGTGCTTCGGAACAAGATGGAGGCTGAGTTCAGATGGTGGGACGAAGTTGACAAGTTTGTTCTGCTCGGTGCGGTTCCATTTCCCAAAGATGTTCCCCAGTTGAAGCGGCTGGGTGTTGGTGGTGTGATCACTCTCAATGAACCTTATGAAACTTTGGTTTCAACATCCTTGTATCGTGCCCATGGAATTGACCATCTAGTAATTCCCACGCGAGATTATCTTTTTGCCCCTTCATTTGTTGATATCAACAGAGCTGTAGAATTCATTCACAAGAATGCATCATGTGGTGAAATTACTTACGTGCACTGTAAAGCTGGTCGGGGAAGGAGCACAACTATTGTGCTTTGCTATTTGGTGAAATACAAGAACATGACACCATGTGCAGCCCTGGACTATGTTCGGTCTAGAAGACCTCGTGTGCTGCTAGCCCCCTCACAATGGAAGGCTGTTCAAGAATACAGCAGATGTGGACCAACTACCACACCATGTTCTCCCTCCGGAGATGCAGTTTTAATTACCAAAGCTGATCTTGAAGGGTACCATGGAACTTACGACGACACTGCTCGTAAGCAGTTGGCAATTGTTTCTAAAATGGTGAAAGCCAGCCCCATGATAGCTAGATTATCGTTCCTTTTTGCATCCTTGAAAGTCTCCGGAAATTGTGGACCAGTCACGAGGCAGTTGCCTGTGCCGGAACCACGTGCTTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002791 GO:0002792 GO:0003674 GO:0003824 GO:0004439 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006629 GO:0006644 GO:0006650 GO:0006655 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0008962 GO:0009058 GO:0009987 GO:0010646 GO:0010648 GO:0010817 GO:0016020 GO:0016021 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0019866 GO:0023051 GO:0023057 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031304 GO:0031305 GO:0031966 GO:0031967 GO:0031975 GO:0032048 GO:0032049 GO:0032592 GO:0032879 GO:0032880 GO:0034593 GO:0034595 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045017 GO:0046471 GO:0046474 GO:0046486 GO:0046676 GO:0046883 GO:0046888 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050708 GO:0050709 GO:0050789 GO:0050794 GO:0050796 GO:0051046 GO:0051048 GO:0051049 GO:0051051 GO:0051223 GO:0051224 GO:0052866 GO:0060341 GO:0061178 GO:0061179 GO:0065007 GO:0065008 GO:0070201 GO:0071704 GO:0090087 GO:0090276 GO:0090278 GO:0090407 GO:0098573 GO:0106019 GO:1901576 GO:1903530 GO:1903531 GO:1904950

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DSPc

Protein Analysis

287

Amino Acids

31.73

Weight (kDa)

9.08

Isoelectric Point (pI)

42.08

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009543)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56610 AT5G56610
fragaria_vesca	FvH4_1g06520 FvH4_1g06520 FvH4_1g06520
malus_domestica	MD02G1068600.v1.1 MD15G1200100.v1.1
prunus_persica	Prupe.7G217100_v2.0.a1 Prupe.7G217100_v2.0.a1
pyrus_communis	pycom02g05430 pycom15g17760
rosa_chinensis	RchiOBHm_Chr2g0092271
rosa_laevigata	RLG00000016292
rosa_multiflora	Rmu_sc0003289.1_g000030
rosa_roxburghii	Rroxscaffold_2G00149310
rosa_rugosa	Rorug02G0024200 Rorug02G0024300
rosa_samantha	Rh2AG070200 Rh2BG069700 Rh2CG070900 Rh2DG068700
rosa_wichuraiana	Rw2G006210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	58
Acc65I	GGTACC	1 cut(s)	687
AccB1I	GGYRCC	1 cut(s)	687
AccII	CGCG	2 cut(s)	118, 361
AccIII	TCCGGA	2 cut(s)	647, 803
AciI	CCGC	6 cut(s)	23, 36, 64, 118, 221, 256
AclWI	GGATC	2 cut(s)	115, 128
AcsI	RAATTY	1 cut(s)	410
AcvI	CACGTG	1 cut(s)	851
AcyI	GRCGYC	1 cut(s)	51
AdeI	CACNNNGTG	1 cut(s)	530
AfaI	GTAC	1 cut(s)	689
AfeI	AGCGCT	1 cut(s)	99
AfiI	CCNNNNNNNGG	3 cut(s)	60, 117, 588
AgsI	TTSAA	5 cut(s)	253, 312, 602, 683, 796
AjnI	CCWGG	1 cut(s)	537
AloI	GAACNNNNNNTCC	2 cut(s)	161, 193
AluBI	AGCT	4 cut(s)	404, 464, 674, 767
AluI	AGCT	4 cut(s)	404, 464, 674, 767
Alw21I	GWGCWC	2 cut(s)	456, 482
Alw26I	GTCTC	1 cut(s)	805
Alw44I	GTGCAC	1 cut(s)	452
AlwI	GGATC	2 cut(s)	115, 128
Aor13HI	TCCGGA	2 cut(s)	647, 803
Aor51HI	AGCGCT	1 cut(s)	99
ApaLI	GTGCAC	1 cut(s)	452
ApeKI	GCWGC	2 cut(s)	533, 571
ApoI	RAATTY	1 cut(s)	410
Asp700I	GAANNNNTTC	1 cut(s)	414
Asp718I	GGTACC	1 cut(s)	687
AspLEI	GCGC	2 cut(s)	100, 118
AspS9I	GGNCC	2 cut(s)	620, 815
AsuC2I	CCSGG	2 cut(s)	61, 112
AsuHPI	GGTGA	3 cut(s)	449, 517, 757
AsuNHI	GCTAGC	1 cut(s)	574
AvaII	GGWCC	2 cut(s)	620, 815
BaeGI	GKGCMC	2 cut(s)	331, 456
BamHI	GGATCC	1 cut(s)	120
BanI	GGYRCC	1 cut(s)	687
BauI	CACGAG	2 cut(s)	564, 823
BbrPI	CACGTG	1 cut(s)	851
BbsI	GAAGAC	1 cut(s)	565
Bbv12I	GWGCWC	2 cut(s)	456, 482
BbvI	GCAGC	2 cut(s)	545, 558
BccI	CCATC	3 cut(s)	160, 177, 351
BceAI	ACGGC	1 cut(s)	38
BcgI	CGANNNNNNTGC	4 cut(s)	692, 726, 814, 848
BciT130I	CCWGG	1 cut(s)	539
BclI	TGATCA	1 cut(s)	276
BcnI	CCSGG	2 cut(s)	61, 112
BcoDI	GTCTC	1 cut(s)	805
BfaI	CTAG	4 cut(s)	347, 555, 575, 768
BfmI	CTRYAG	1 cut(s)	405
BfoI	RGCGCY	1 cut(s)	101
BfuAI	ACCTGC	1 cut(s)	58
BisI	GCNGC	5 cut(s)	37, 65, 257, 534, 572
BlsI	GCNGC	5 cut(s)	38, 66, 258, 535, 573
Bme1390I	CCNGG	3 cut(s)	61, 112, 539
Bme18I	GGWCC	2 cut(s)	620, 815
BmgT120I	GGNCC	2 cut(s)	620, 815
BmiI	GGNNCC	5 cut(s)	109, 122, 225, 689, 846
BmrFI	CCNGG	3 cut(s)	61, 112, 539
BmrI	ACTGGG	1 cut(s)	241
BmsI	GCATC	3 cut(s)	437, 643, 797
BmtI	GCTAGC	1 cut(s)	578
BmuI	ACTGGG	1 cut(s)	241
BpiI	GAAGAC	1 cut(s)	565
BpuMI	CCSGG	2 cut(s)	61, 112
BsaAI	YACGTR	2 cut(s)	451, 851
BsaHI	GRCGYC	1 cut(s)	51
BsaJI	CCNNGG	4 cut(s)	111, 331, 537, 691
BsaWI	WCCGGW	2 cut(s)	647, 803
BsaXI	ACNNNNNCTCC	4 cut(s)	161, 191, 642, 672
Bsc4I	CCNNNNNNNGG	3 cut(s)	60, 117, 588
Bse1I	ACTGG	2 cut(s)	247, 818
BseAI	TCCGGA	2 cut(s)	647, 803
BseBI	CCWGG	1 cut(s)	539
BseDI	CCNNGG	4 cut(s)	111, 331, 537, 691
BseGI	GGATG	2 cut(s)	314, 788
BseLI	CCNNNNNNNGG	3 cut(s)	60, 117, 588
BseMII	CTCAG	1 cut(s)	165
BseNI	ACTGG	2 cut(s)	247, 818
BseRI	GAGGAG	1 cut(s)	26
BseSI	GKGCMC	2 cut(s)	331, 456
BseXI	GCAGC	2 cut(s)	545, 558
BseYI	CCCAGC	1 cut(s)	259
BsgI	GTGCAG	1 cut(s)	552
Bsh1236I	CGCG	2 cut(s)	118, 361
BshNI	GGYRCC	1 cut(s)	687
BsiHKAI	GWGCWC	2 cut(s)	456, 482
BsiSI	CCGG	5 cut(s)	60, 111, 648, 804, 842
BslFI	GGGAC	1 cut(s)	203
BslI	CCNNNNNNNGG	3 cut(s)	60, 117, 588
BsmAI	GTCTC	1 cut(s)	805
BsmFI	GGGAC	1 cut(s)	203
BsmI	GAATGC	1 cut(s)	430
Bsp1286I	GDGCHC	3 cut(s)	331, 456, 482
Bsp13I	TCCGGA	2 cut(s)	647, 803
Bsp143I	GATC	4 cut(s)	6, 120, 276, 676
Bsp19I	CCATGG	2 cut(s)	331, 691
BspACI	CCGC	6 cut(s)	23, 36, 64, 118, 221, 256
BspCNI	CTCAG	1 cut(s)	166
BspEI	TCCGGA	2 cut(s)	647, 803
BspFNI	CGCG	2 cut(s)	118, 361
BspLI	GGNNCC	5 cut(s)	109, 122, 225, 689, 846
BspMI	ACCTGC	1 cut(s)	58
BspOI	GCTAGC	1 cut(s)	578
BspPI	GGATC	2 cut(s)	115, 128
BspT107I	GGYRCC	1 cut(s)	687
BsrI	ACTGG	2 cut(s)	247, 818
BssECI	CCNNGG	4 cut(s)	111, 331, 537, 691
BssMI	GATC	4 cut(s)	6, 120, 276, 676
BssNI	GRCGYC	1 cut(s)	51
BssSI	CACGAG	2 cut(s)	564, 823
BssT1I	CCWWGG	2 cut(s)	331, 691
Bst2BI	CACGAG	2 cut(s)	564, 823
Bst2UI	CCWGG	1 cut(s)	539
Bst4CI	ACNGT	1 cut(s)	458
BstACI	GRCGYC	1 cut(s)	51
BstBAI	YACGTR	2 cut(s)	451, 851
BstC8I	GCNNGC	3 cut(s)	576, 754, 856
BstDEI	CTNAG	2 cut(s)	90, 174
BstDSI	CCRYGG	2 cut(s)	331, 691
BstF5I	GGATG	2 cut(s)	314, 788
BstFNI	CGCG	2 cut(s)	118, 361
BstH2I	RGCGCY	1 cut(s)	101
BstHHI	GCGC	2 cut(s)	100, 118
BstKTI	GATC	4 cut(s)	9, 123, 279, 679
BstMAI	GTCTC	1 cut(s)	805
BstMBI	GATC	4 cut(s)	6, 120, 276, 676
BstMWI	GCNNNNNNNGC	1 cut(s)	95
BstNI	CCWGG	1 cut(s)	539
BstSCI	CCNGG	3 cut(s)	59, 110, 537
BstSFI	CTRYAG	1 cut(s)	405
BstSLI	GKGCMC	2 cut(s)	331, 456
BstUI	CGCG	2 cut(s)	118, 361
BstV1I	GCAGC	2 cut(s)	545, 558
BstV2I	GAAGAC	1 cut(s)	565
BstX2I	RGATCY	1 cut(s)	120
BstYI	RGATCY	1 cut(s)	120
BtgI	CCRYGG	2 cut(s)	331, 691
BtsCI	GGATG	2 cut(s)	314, 788
BtsI	GCAGTG	1 cut(s)	708
BtsIMutI	CAGTG	2 cut(s)	454, 708
BveI	ACCTGC	1 cut(s)	58
Cac8I	GCNNGC	3 cut(s)	576, 754, 856
CfoI	GCGC	2 cut(s)	100, 118
Cfr13I	GGNCC	2 cut(s)	620, 815
CseI	GACGC	1 cut(s)	59
Csp6I	GTAC	1 cut(s)	688
CviAII	CATG	7 cut(s)	332, 432, 520, 528, 636, 692, 760
CviQI	GTAC	1 cut(s)	688
DdeI	CTNAG	2 cut(s)	90, 174
DpnI	GATC	4 cut(s)	8, 122, 278, 678
DpnII	GATC	4 cut(s)	6, 120, 276, 676
DraIII	CACNNNGTG	1 cut(s)	530
EciI	GGCGGA	1 cut(s)	38
Eco130I	CCWWGG	2 cut(s)	331, 691
Eco32I	GATATC	1 cut(s)	394
Eco47I	GGWCC	2 cut(s)	620, 815
Eco47III	AGCGCT	1 cut(s)	99
Eco72I	CACGTG	1 cut(s)	851
EcoRI	GAATTC	1 cut(s)	410
EcoRII	CCWGG	1 cut(s)	537
EcoRV	GATATC	1 cut(s)	394
EcoT14I	CCWWGG	2 cut(s)	331, 691
EcoT22I	ATGCAT	1 cut(s)	430
ErhI	CCWWGG	2 cut(s)	331, 691
FaeI	CATG	7 cut(s)	335, 435, 523, 531, 639, 695, 763
FalI	AAGNNNNNCTT	2 cut(s)	663, 695
FaqI	GGGAC	1 cut(s)	203
FatI	CATG	7 cut(s)	331, 431, 519, 527, 635, 691, 759
FbaI	TGATCA	1 cut(s)	276
Fnu4HI	GCNGC	5 cut(s)	37, 65, 257, 534, 572
FokI	GGATG	2 cut(s)	301, 775
Fsp4HI	GCNGC	5 cut(s)	37, 65, 257, 534, 572
FspBI	CTAG	4 cut(s)	347, 555, 575, 768
GlaI	GCGC	2 cut(s)	99, 117
GluI	GCNGC	5 cut(s)	37, 65, 257, 534, 572
GsaI	CCCAGC	1 cut(s)	263
HaeII	RGCGCY	1 cut(s)	101
HapII	CCGG	5 cut(s)	60, 111, 648, 804, 842
HgaI	GACGC	1 cut(s)	59
HhaI	GCGC	2 cut(s)	100, 118
Hin1I	GRCGYC	1 cut(s)	51
Hin1II	CATG	7 cut(s)	335, 435, 523, 531, 639, 695, 763
Hin6I	GCGC	2 cut(s)	98, 116
HinP1I	GCGC	2 cut(s)	98, 116
HincII	GTYRAC	2 cut(s)	82, 199
HindII	GTYRAC	2 cut(s)	82, 199
HpaII	CCGG	5 cut(s)	60, 111, 648, 804, 842
HphI	GGTGA	3 cut(s)	449, 517, 757
Hpy166II	GTNNAC	5 cut(s)	82, 199, 454, 620, 815
Hpy188I	TCNGA	3 cut(s)	45, 159, 182
Hpy188III	TCNNGA	6 cut(s)	555, 602, 648, 680, 804, 823
Hpy8I	GTNNAC	5 cut(s)	82, 199, 454, 620, 815
Hpy99I	CGWCG	2 cut(s)	53, 707
HpyAV	CCTTC	4 cut(s)	390, 468, 586, 677
HpyCH4III	ACNGT	1 cut(s)	458
HpyCH4IV	ACGT	2 cut(s)	450, 850
HpyCH4V	TGCA	5 cut(s)	428, 454, 533, 656, 788
HpyF10VI	GCNNNNNNNGC	1 cut(s)	95
HpyF3I	CTNAG	2 cut(s)	90, 174
HpySE526I	ACGT	2 cut(s)	450, 850
Hsp92I	GRCGYC	1 cut(s)	51
Hsp92II	CATG	7 cut(s)	335, 435, 523, 531, 639, 695, 763
HspAI	GCGC	2 cut(s)	98, 116
Kpn2I	TCCGGA	2 cut(s)	647, 803
KpnI	GGTACC	1 cut(s)	691
Ksp22I	TGATCA	1 cut(s)	276
Kzo9I	GATC	4 cut(s)	6, 120, 276, 676
LmnI	GCTCC	2 cut(s)	107, 477
Lsp1109I	GCAGC	2 cut(s)	545, 558
LweI	GCATC	3 cut(s)	437, 643, 797
MaeI	CTAG	4 cut(s)	347, 555, 575, 768
MaeII	ACGT	2 cut(s)	450, 850
MaeIII	GTNAC	1 cut(s)	820
MalI	GATC	4 cut(s)	8, 122, 278, 678
MboI	GATC	4 cut(s)	6, 120, 276, 676
MboII	GAAGA	2 cut(s)	16, 570
MfeI	CAATTG	1 cut(s)	729
MflI	RGATCY	1 cut(s)	120
MhlI	GDGCHC	3 cut(s)	331, 456, 482
MluCI	AATT	7 cut(s)	336, 351, 410, 441, 663, 729, 808
MmeI	TCCRAC	1 cut(s)	85
MnlI	CCTC	9 cut(s)	4, 13, 19, 79, 163, 573, 592, 655, 819
Mph1103I	ATGCAT	1 cut(s)	430
MroI	TCCGGA	2 cut(s)	647, 803
MroXI	GAANNNNTTC	1 cut(s)	414
MseI	TTAA	1 cut(s)	662
MslI	CAYNNNNRTG	1 cut(s)	423
MspI	CCGG	5 cut(s)	60, 111, 648, 804, 842
MspR9I	CCNGG	3 cut(s)	61, 112, 539
MunI	CAATTG	1 cut(s)	729
Mva1269I	GAATGC	1 cut(s)	430
MvaI	CCWGG	1 cut(s)	539
MvnI	CGCG	2 cut(s)	118, 361
MwoI	GCNNNNNNNGC	1 cut(s)	95
NciI	CCSGG	2 cut(s)	61, 112
NcoI	CCATGG	2 cut(s)	331, 691
NdeII	GATC	4 cut(s)	6, 120, 276, 676
NheI	GCTAGC	1 cut(s)	574
NlaIII	CATG	7 cut(s)	335, 435, 523, 531, 639, 695, 763
NlaIV	GGNNCC	5 cut(s)	109, 122, 225, 689, 846
NmuCI	GTSAC	1 cut(s)	820
NsiI	ATGCAT	1 cut(s)	430
PctI	GAATGC	1 cut(s)	430
PdmI	GAANNNNTTC	1 cut(s)	414
PkrI	GCNGC	5 cut(s)	38, 66, 258, 535, 573
PmaCI	CACGTG	1 cut(s)	851
PmlI	CACGTG	1 cut(s)	851
Ppu21I	YACGTR	2 cut(s)	451, 851
Psp6I	CCWGG	1 cut(s)	537
PspCI	CACGTG	1 cut(s)	851
PspFI	CCCAGC	1 cut(s)	259
PspGI	CCWGG	1 cut(s)	537
PspN4I	GGNNCC	5 cut(s)	109, 122, 225, 689, 846
PspPI	GGNCC	2 cut(s)	620, 815
PsuI	RGATCY	1 cut(s)	120
RsaI	GTAC	1 cut(s)	689
RsaNI	GTAC	1 cut(s)	688
RseI	CAYNNNNRTG	1 cut(s)	423
SaqAI	TTAA	1 cut(s)	662
SatI	GCNGC	5 cut(s)	37, 65, 257, 534, 572
Sau3AI	GATC	4 cut(s)	6, 120, 276, 676
Sau96I	GGNCC	2 cut(s)	620, 815
ScrFI	CCNGG	3 cut(s)	61, 112, 539
SduI	GDGCHC	3 cut(s)	331, 456, 482
SetI	ASST	9 cut(s)	72, 295, 406, 453, 466, 565, 676, 769, 853
SfaNI	GCATC	3 cut(s)	437, 643, 797
SfcI	CTRYAG	1 cut(s)	405
SinI	GGWCC	2 cut(s)	620, 815
SmiMI	CAYNNNNRTG	1 cut(s)	423
Sse9I	AATT	7 cut(s)	336, 351, 410, 441, 663, 729, 808
SsiI	CCGC	6 cut(s)	23, 36, 64, 118, 221, 256
SspMI	CTAG	4 cut(s)	347, 555, 575, 768
StyD4I	CCNGG	3 cut(s)	59, 110, 537
StyI	CCWWGG	2 cut(s)	331, 691
TaaI	ACNGT	1 cut(s)	458
TaiI	ACGT	2 cut(s)	453, 853
TaqI	TCGA	1 cut(s)	9
TaqII	GACCGA	1 cut(s)	540
TasI	AATT	7 cut(s)	336, 351, 410, 441, 663, 729, 808
TauI	GCSGC	3 cut(s)	39, 67, 259
Tru1I	TTAA	1 cut(s)	662
Tru9I	TTAA	1 cut(s)	662
TscAI	CASTG	2 cut(s)	461, 715
TseFI	GTSAC	1 cut(s)	820
TseI	GCWGC	2 cut(s)	533, 571
Tsp45I	GTSAC	1 cut(s)	820
TspDTI	ATGAA	5 cut(s)	17, 303, 312, 372, 403
TspRI	CASTG	2 cut(s)	461, 715
VneI	GTGCAC	1 cut(s)	452
VpaK11BI	GGWCC	2 cut(s)	620, 815
XapI	RAATTY	1 cut(s)	410
XbaI	TCTAGA	1 cut(s)	554
XmnI	GAANNNNTTC	1 cut(s)	414
XspI	CTAG	4 cut(s)	347, 555, 575, 768
Zsp2I	ATGCAT	1 cut(s)	430

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.