pycom02g06730

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011798
Physical Location & Seq
Forward (+)
4676085 .. 4679326
3242 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1137 bp
ATGGAGAACCATAAGCAGATGGAGAAGGACGAGGCGGGGCGCATGGCGGTGGAGGAGGAGGACGGGCAGGTGGGGTCCAGCCTGACCCTGGAGAGGGTGGCTGCGGCCAAGCAGTTCATAGAGAGCCACTACAAGGCTCAGATGAAGCACATCCAGGATCGTAAACAGAGGCGCTCAGTGCTGGAAAAGAAGTTGGCATCTGAACATGTACCGGAGGAGGAACAGATCAATTTGCTGAAGGATTTGGAGCTCACAGAGACGCAATACATACGCCTTAAAAGGCACAAAATTTGTGTGGATGATTTTGACCTTCTGGCCATAATTGGGAGAGGGGCCTTTGGAGAGGTTAGACTGTGCCGGGAGAAGAAATCTGGCAACATTTATGCCATGAAAAAGTTGAAGAAGTCAGAAATGCTCAGTAGGGGGCAGGTTGAACATGTTAGAGCCGAAAGGAATTTGCTTGCAGAGGTTGCCAGTCATTGCATTGTGAAACTTTACTATTCCTTTCAAGATGCTGAATACTTGTATCTGGTCATGGAATATCTACCTGGTGGCGACATGATGACTTTGCTTATTAGGGAAGAGTCTTTGACTGAAACTGTCGCAAGATTTTACATTGCGCAAAGTGTTTTGGCCATAGAATCTATTCATAAACATAACTATATTCACAGAGACATAAAACCAGACAATCTCCTGTTAGACAAAAATGGTCACATGAAGCTCTCTGATTTTGGTCTTTGCAAGCCTCTCGACTGCTCGAATTTATCTTCCATAAATGAAAATGAAGTGCTTGATGATGAGAACCTGAATGAGGCAATGGATATTGATGAATCCTTCCCTCAAACAAAAAATGGGAGGCGCTGGAAAAGCCCGCTTGAGCAACTTCAGCATTGGCAGATAAACAGGAGAAAATTGGCATTTTCGACAGTTGGAACTCCAGATTACATCGCTCCAGAGGTATTGTTGAAGAAGGGATACGGCATGGAGTGTGACTGGTGGTCACTTGGTGCGATAATGTATGAGATGCTTGTTGGTTATCCTCCATTTTACTCTGATCGATCCCGTTACAACATGCAGAAAGATTGTACATTGGAAAAATCACTTGAAATTTCCTGTCGAGGCACGGTTGACACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

379

Amino Acids

43.84

Weight (kDa)

5.91

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 104 - 253 1e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 105 - 252 1.8e-23 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 176 - 251 7.2e-07 ABC1 atypical kinase-like domain
Pkinase PF00069 302 - 361 7.6e-15 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 58
Acc16I TGCGCA 1 cut(s) 621
Acc36I ACCTGC 2 cut(s) 58, 418
AciI CCGC 4 cut(s) 35, 47, 104, 872
AclWI GGATC 2 cut(s) 165, 1053
AcoI YGGCCR 3 cut(s) 105, 315, 633
AcsI RAATTY 4 cut(s) 288, 454, 760, 1107
AcuI CTGAAG 2 cut(s) 257, 869
AdeI CACNNNGTG 1 cut(s) 1007
AfaI GTAC 2 cut(s) 210, 1087
AfiI CCNNNNNNNGG 6 cut(s) 88, 93, 94, 133, 324, 811
AflIII ACRYGT 2 cut(s) 205, 436
AgsI TTSAA 5 cut(s) 400, 434, 509, 967, 1106
AhdI GACNNNNNGTC 1 cut(s) 997
AjnI CCWGG 3 cut(s) 87, 153, 547
AluBI AGCT 2 cut(s) 250, 721
AluI AGCT 2 cut(s) 250, 721
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 2 cut(s) 251, 666
AlwI GGATC 2 cut(s) 165, 1053
AoxI GGCC 4 cut(s) 105, 315, 333, 633
ApeKI GCWGC 1 cut(s) 101
ApoI RAATTY 4 cut(s) 288, 454, 760, 1107
Asp700I GAANNNNTTC 1 cut(s) 645
AspLEI GCGC 4 cut(s) 42, 174, 622, 861
AspS9I GGNCC 2 cut(s) 75, 333
AsuC2I CCSGG 1 cut(s) 359
AvaII GGWCC 1 cut(s) 75
BalI TGGCCA 2 cut(s) 317, 635
BanII GRGCYC 1 cut(s) 252
BarI GAAGNNNNNNTAC 2 cut(s) 959, 991
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 1 cut(s) 88
BccI CCATC 1 cut(s) 13
BceAI ACGGC 1 cut(s) 994
BcgI CGANNNNNNTGC 2 cut(s) 730, 764
BciT130I CCWGG 3 cut(s) 89, 155, 549
BciVI GTATCC 1 cut(s) 968
BcnI CCSGG 1 cut(s) 359
BcoDI GTCTC 2 cut(s) 251, 666
BfoI RGCGCY 2 cut(s) 175, 862
BfuAI ACCTGC 2 cut(s) 58, 418
BfuI GTATCC 1 cut(s) 968
BisI GCNGC 2 cut(s) 102, 105
BlsI GCNGC 2 cut(s) 103, 106
Bme1390I CCNGG 4 cut(s) 89, 155, 359, 549
Bme18I GGWCC 1 cut(s) 75
BmeRI GACNNNNNGTC 1 cut(s) 997
BmgT120I GGNCC 2 cut(s) 75, 333
BmiI GGNNCC 2 cut(s) 76, 334
BmrFI CCNGG 4 cut(s) 89, 155, 359, 549
BmsI GCATC 3 cut(s) 206, 502, 1014
BpmI CTGGAG 3 cut(s) 110, 921, 936
BpuEI CTTGAG 1 cut(s) 896
BpuMI CCSGG 1 cut(s) 359
Bsa29I ATCGAT 1 cut(s) 1057
BsaJI CCNNGG 1 cut(s) 87
BsaWI WCCGGW 1 cut(s) 211
Bsc4I CCNNNNNNNGG 6 cut(s) 88, 93, 94, 133, 324, 811
Bse1I ACTGG 2 cut(s) 474, 998
Bse3DI GCAATG 3 cut(s) 478, 615, 822
BseBI CCWGG 3 cut(s) 89, 155, 549
BseCI ATCGAT 1 cut(s) 1057
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 2 cut(s) 150, 304
BseLI CCNNNNNNNGG 6 cut(s) 88, 93, 94, 133, 324, 811
BseMI GCAATG 3 cut(s) 478, 615, 822
BseMII CTCAG 3 cut(s) 152, 189, 430
BseNI ACTGG 2 cut(s) 474, 998
BseRI GAGGAG 3 cut(s) 68, 71, 230
BseXI GCAGC 1 cut(s) 88
BshFI GGCC 4 cut(s) 107, 317, 335, 635
BshVI ATCGAT 1 cut(s) 1057
BsiHKAI GWGCWC 1 cut(s) 252
BsiSI CCGG 2 cut(s) 212, 358
BslI CCNNNNNNNGG 6 cut(s) 88, 93, 94, 133, 324, 811
BsmAI GTCTC 2 cut(s) 251, 666
BsmBI CGTCTC 1 cut(s) 251
BsnI GGCC 4 cut(s) 107, 317, 335, 635
Bsp1286I GDGCHC 1 cut(s) 252
Bsp1407I TGTACA 1 cut(s) 1085
Bsp143I GATC 4 cut(s) 157, 225, 1054, 1058
BspACI CCGC 4 cut(s) 35, 47, 104, 872
BspANI GGCC 4 cut(s) 107, 317, 335, 635
BspCNI CTCAG 3 cut(s) 151, 188, 429
BspDI ATCGAT 1 cut(s) 1057
BspLI GGNNCC 2 cut(s) 76, 334
BspMI ACCTGC 2 cut(s) 58, 418
BspPI GGATC 2 cut(s) 165, 1053
BsrDI GCAATG 3 cut(s) 478, 615, 822
BsrGI TGTACA 1 cut(s) 1085
BsrI ACTGG 2 cut(s) 474, 998
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 4 cut(s) 157, 225, 1054, 1058
Bst2UI CCWGG 3 cut(s) 89, 155, 549
Bst4CI ACNGT 4 cut(s) 354, 601, 928, 1126
Bst6I CTCTTC 1 cut(s) 576
BstAPI GCANNNNNTGC 1 cut(s) 470
BstAUI TGTACA 1 cut(s) 1085
BstC8I GCNNGC 3 cut(s) 462, 743, 872
BstDEI CTNAG 3 cut(s) 138, 175, 416
BstENI CCTNNNNNAGG 1 cut(s) 809
BstF5I GGATG 2 cut(s) 150, 304
BstH2I RGCGCY 2 cut(s) 175, 862
BstHHI GCGC 4 cut(s) 42, 174, 622, 861
BstKTI GATC 4 cut(s) 160, 228, 1057, 1061
BstMAI GTCTC 2 cut(s) 251, 666
BstMBI GATC 4 cut(s) 157, 225, 1054, 1058
BstMWI GCNNNNNNNGC 4 cut(s) 178, 470, 867, 886
BstNI CCWGG 3 cut(s) 89, 155, 549
BstNSI RCATGY 3 cut(s) 209, 440, 1075
BstSCI CCNGG 4 cut(s) 87, 153, 357, 547
BstV1I GCAGC 1 cut(s) 88
Bsu15I ATCGAT 1 cut(s) 1057
BsuI GTATCC 1 cut(s) 968
BsuRI GGCC 4 cut(s) 107, 317, 335, 635
BsuTUI ATCGAT 1 cut(s) 1057
BtgZI GCGATG 1 cut(s) 931
BtsCI GGATG 2 cut(s) 150, 304
BtsIMutI CAGTG 1 cut(s) 183
BveI ACCTGC 2 cut(s) 58, 418
Cac8I GCNNGC 3 cut(s) 462, 743, 872
CfoI GCGC 4 cut(s) 42, 174, 622, 861
Cfr13I GGNCC 2 cut(s) 75, 333
ClaI ATCGAT 1 cut(s) 1057
CseI GACGC 1 cut(s) 268
CsiI ACCWGGT 1 cut(s) 547
Csp6I GTAC 2 cut(s) 209, 1086
CviAII CATG 9 cut(s) 43, 206, 388, 437, 535, 559, 715, 982, 1072
CviQI GTAC 2 cut(s) 209, 1086
DdeI CTNAG 3 cut(s) 138, 175, 416
DpnI GATC 4 cut(s) 159, 227, 1056, 1060
DpnII GATC 4 cut(s) 157, 225, 1054, 1058
DraIII CACNNNGTG 1 cut(s) 1007
DriI GACNNNNNGTC 1 cut(s) 997
EaeI YGGCCR 3 cut(s) 105, 315, 633
Eam1104I CTCTTC 1 cut(s) 576
Eam1105I GACNNNNNGTC 1 cut(s) 997
EarI CTCTTC 1 cut(s) 576
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 75
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 2 cut(s) 257, 869
EcoICRI GAGCTC 1 cut(s) 250
EcoNI CCTNNNNNAGG 1 cut(s) 809
EcoO109I RGGNCCY 1 cut(s) 333
EcoRII CCWGG 3 cut(s) 87, 153, 547
EcoT38I GRGCYC 1 cut(s) 252
Esp3I CGTCTC 1 cut(s) 251
FaeI CATG 9 cut(s) 46, 209, 391, 440, 538, 562, 718, 985, 1075
FatI CATG 9 cut(s) 42, 205, 387, 436, 534, 558, 714, 981, 1071
FauI CCCGC 2 cut(s) 28, 879
Fnu4HI GCNGC 2 cut(s) 102, 105
FokI GGATG 2 cut(s) 137, 311
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 2 cut(s) 102, 105
FspI TGCGCA 1 cut(s) 621
GlaI GCGC 4 cut(s) 41, 173, 621, 860
GluI GCNGC 2 cut(s) 102, 105
GsuI CTGGAG 3 cut(s) 110, 921, 936
HaeII RGCGCY 2 cut(s) 175, 862
HaeIII GGCC 4 cut(s) 107, 317, 335, 635
HapII CCGG 2 cut(s) 212, 358
HgaI GACGC 1 cut(s) 268
HhaI GCGC 4 cut(s) 42, 174, 622, 861
Hin1II CATG 9 cut(s) 46, 209, 391, 440, 538, 562, 718, 985, 1075
Hin6I GCGC 4 cut(s) 40, 172, 620, 859
HinP1I GCGC 4 cut(s) 40, 172, 620, 859
HincII GTYRAC 1 cut(s) 1129
HindII GTYRAC 1 cut(s) 1129
HinfI GANTC 3 cut(s) 584, 641, 830
HpaII CCGG 2 cut(s) 212, 358
Hpy166II GTNNAC 2 cut(s) 164, 1129
Hpy188I TCNGA 5 cut(s) 141, 202, 409, 727, 1054
Hpy188III TCNNGA 4 cut(s) 509, 749, 938, 953
Hpy8I GTNNAC 2 cut(s) 164, 1129
HpyAV CCTTC 5 cut(s) 19, 232, 320, 844, 964
HpyCH4III ACNGT 4 cut(s) 354, 601, 928, 1126
HpyCH4V TGCA 4 cut(s) 464, 483, 741, 1075
HpyF10VI GCNNNNNNNGC 4 cut(s) 178, 470, 867, 886
HpyF3I CTNAG 3 cut(s) 138, 175, 416
Hsp92II CATG 9 cut(s) 46, 209, 391, 440, 538, 562, 718, 985, 1075
HspAI GCGC 4 cut(s) 40, 172, 620, 859
Kzo9I GATC 4 cut(s) 157, 225, 1054, 1058
LmnI GCTCC 2 cut(s) 247, 955
Lsp1109I GCAGC 1 cut(s) 88
LweI GCATC 3 cut(s) 206, 502, 1014
MabI ACCWGGT 1 cut(s) 547
MaeIII GTNAC 4 cut(s) 710, 989, 999, 1064
MalI GATC 4 cut(s) 159, 227, 1056, 1060
MboI GATC 4 cut(s) 157, 225, 1054, 1058
MboII GAAGA 5 cut(s) 376, 412, 593, 759, 979
MhlI GDGCHC 1 cut(s) 252
MlsI TGGCCA 2 cut(s) 317, 635
MluCI AATT 7 cut(s) 229, 288, 321, 454, 760, 911, 1107
MluNI TGGCCA 2 cut(s) 317, 635
MlyI GAGTC 1 cut(s) 593
MmeI TCCRAC 1 cut(s) 910
Mox20I TGGCCA 2 cut(s) 317, 635
MroXI GAANNNNTTC 1 cut(s) 645
MscI TGGCCA 2 cut(s) 317, 635
MseI TTAA 1 cut(s) 276
MslI CAYNNNNRTG 1 cut(s) 47
Msp20I TGGCCA 2 cut(s) 317, 635
MspI CCGG 2 cut(s) 212, 358
MspR9I CCNGG 4 cut(s) 89, 155, 359, 549
MvaI CCWGG 3 cut(s) 89, 155, 549
MwoI GCNNNNNNNGC 4 cut(s) 178, 470, 867, 886
NciI CCSGG 1 cut(s) 359
NdeII GATC 4 cut(s) 157, 225, 1054, 1058
NlaIII CATG 9 cut(s) 46, 209, 391, 440, 538, 562, 718, 985, 1075
NlaIV GGNNCC 2 cut(s) 76, 334
NmuCI GTSAC 3 cut(s) 710, 989, 999
NsbI TGCGCA 1 cut(s) 621
NspI RCATGY 3 cut(s) 209, 440, 1075
PaqCI CACCTGC 1 cut(s) 58
PciI ACATGT 2 cut(s) 205, 436
PdmI GAANNNNTTC 1 cut(s) 645
PfeI GAWTC 2 cut(s) 641, 830
PfoI TCCNGGA 1 cut(s) 153
PkrI GCNGC 2 cut(s) 103, 106
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
PscI ACATGT 2 cut(s) 205, 436
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 3 cut(s) 87, 153, 547
PspGI CCWGG 3 cut(s) 87, 153, 547
PspN4I GGNNCC 2 cut(s) 76, 334
PspPI GGNCC 2 cut(s) 75, 333
RsaI GTAC 2 cut(s) 210, 1087
RsaNI GTAC 2 cut(s) 209, 1086
RseI CAYNNNNRTG 1 cut(s) 47
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 2 cut(s) 102, 105
Sau3AI GATC 4 cut(s) 157, 225, 1054, 1058
Sau96I GGNCC 2 cut(s) 75, 333
SchI GAGTC 1 cut(s) 593
ScrFI CCNGG 4 cut(s) 89, 155, 359, 549
SduI GDGCHC 1 cut(s) 252
SexAI ACCWGGT 1 cut(s) 547
SfaNI GCATC 3 cut(s) 206, 502, 1014
SinI GGWCC 1 cut(s) 75
SmiMI CAYNNNNRTG 1 cut(s) 47
SmlI CTYRAG 1 cut(s) 875
SmoI CTYRAG 1 cut(s) 875
Sse9I AATT 7 cut(s) 229, 288, 321, 454, 760, 911, 1107
SsiI CCGC 4 cut(s) 35, 47, 104, 872
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 4 cut(s) 87, 153, 357, 547
TaaI ACNGT 4 cut(s) 354, 601, 928, 1126
TaqI TCGA 5 cut(s) 750, 758, 923, 1057, 1117
TasI AATT 7 cut(s) 229, 288, 321, 454, 760, 911, 1107
TatI WGTACW 1 cut(s) 1085
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 2 cut(s) 641, 830
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 1 cut(s) 183
TseFI GTSAC 3 cut(s) 710, 989, 999
TseI GCWGC 1 cut(s) 101
Tsp45I GTSAC 3 cut(s) 710, 989, 999
TspDTI ATGAA 8 cut(s) 106, 158, 404, 638, 731, 792, 798, 843
TspRI CASTG 1 cut(s) 183
VpaK11BI GGWCC 1 cut(s) 75
XagI CCTNNNNNAGG 1 cut(s) 809
XapI RAATTY 4 cut(s) 288, 454, 760, 1107
XceI RCATGY 3 cut(s) 209, 440, 1075
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XmnI GAANNNNTTC 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.