pycom02g06790

Cleavage and polyadenylation specificity factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012542
Physical Location & Seq
Reverse (-)
4710407 .. 4711952
1546 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 660 bp
ATGAAAGGGGTTCCTTACACGTGTCAGACAGCAACGGTCTTGATCGATCGTGACAGCGATGATCAGAGCCGTACGCTGGACATATACCCTCTCAGCAGCTACTACTTCGCTTCCAAGGAAGCCATTCCTGTCAAGGTCGAAGCTCCGATCGATCGTCTTCACAGATTAAAGTCCAATTATGCTGCTTATGGATTGAGGACTTGTGTGGAAGCGGTTCTTCTGGTTGAGTTGTTCAAACATCCTCATGTGCTACTCTTGCAAATAAGAAACTGCATTTTTAAGCTGCCCGGGGGCCGCCTAAGACCCGGCGAATCAGAGATCAACGGATTGAAACGTAAGCTGTCGAGGAAGCTATGTATCAATGCTGATGGTGATGGGATTCCTTGGGAGGTTGGGGAATGTCTTGGGGCATGGTTGAGGCACGACTTCGAATCATTGCTCTGCCGGGACTTGCCAACCAGAGTTGAAAGCCCAAAGGAGTGCACAAAACTCTACGTTGTCCAGTTACCAGATTGTTGGAAGTTCGTTGCGCCAAAAAACCAAAAACTGCTTGCTATTCCCTTGTGCCAGATCGCCAAAAACAACGAGACGTATGGACCTATAATATCAGGTGTTCCAGAGATGCTATCAAAGTTTACCATCAACATTGTAGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
NUDIX_2

Protein Analysis

220

Amino Acids

24.76

Weight (kDa)

8.32

Isoelectric Point (pI)

38.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX_2 PF13869 25 - 212 8.6e-63 Nucleotide hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 212, 295
AcvI CACGTG 1 cut(s) 21
AfaI GTAC 1 cut(s) 73
AfiI CCNNNNNNNGG 1 cut(s) 76
AflIII ACRYGT 2 cut(s) 18, 20
AgsI TTSAA 3 cut(s) 235, 331, 467
AluBI AGCT 5 cut(s) 99, 143, 283, 340, 352
AluI AGCT 5 cut(s) 99, 143, 283, 340, 352
Alw21I GWGCWC 1 cut(s) 485
Alw26I GTCTC 1 cut(s) 581
Alw44I GTGCAC 1 cut(s) 481
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 1 cut(s) 292
ApaLI GTGCAC 1 cut(s) 481
ApeKI GCWGC 3 cut(s) 96, 182, 283
Asp700I GAANNNNTTC 2 cut(s) 123, 213
AspLEI GCGC 1 cut(s) 532
AspS9I GGNCC 2 cut(s) 292, 596
AsuC2I CCSGG 4 cut(s) 288, 289, 306, 446
AsuHPI GGTGA 1 cut(s) 383
AsuII TTCGAA 1 cut(s) 429
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 596
BaeGI GKGCMC 1 cut(s) 485
BbrPI CACGTG 1 cut(s) 21
BbsI GAAGAC 1 cut(s) 149
Bbv12I GWGCWC 1 cut(s) 485
BbvI GCAGC 3 cut(s) 108, 169, 270
BccI CCATC 3 cut(s) 362, 368, 647
BceAI ACGGC 1 cut(s) 54
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 4 cut(s) 288, 289, 306, 446
BcoDI GTCTC 1 cut(s) 581
BisI GCNGC 4 cut(s) 97, 183, 284, 295
BlsI GCNGC 4 cut(s) 98, 184, 285, 296
Bme1390I CCNGG 4 cut(s) 288, 289, 306, 446
Bme18I GGWCC 1 cut(s) 596
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 2 cut(s) 292, 596
BmiI GGNNCC 2 cut(s) 12, 293
BmrFI CCNGG 4 cut(s) 288, 289, 306, 446
BmsI GCATC 1 cut(s) 612
BpiI GAAGAC 1 cut(s) 149
Bpu14I TTCGAA 1 cut(s) 429
BpuMI CCSGG 4 cut(s) 288, 289, 306, 446
Bsa29I ATCGAT 2 cut(s) 45, 150
BsaAI YACGTR 1 cut(s) 21
BsaJI CCNNGG 4 cut(s) 114, 287, 288, 383
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 502
Bse3DI GCAATG 1 cut(s) 434
BseCI ATCGAT 2 cut(s) 45, 150
BseDI CCNNGG 4 cut(s) 114, 287, 288, 383
BseGI GGATG 1 cut(s) 238
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMI GCAATG 1 cut(s) 434
BseMII CTCAG 1 cut(s) 106
BseNI ACTGG 1 cut(s) 502
BseSI GKGCMC 1 cut(s) 485
BseXI GCAGC 3 cut(s) 108, 169, 270
Bsh1285I CGRYCG 3 cut(s) 49, 150, 154
BshFI GGCC 1 cut(s) 294
BshVI ATCGAT 2 cut(s) 45, 150
BsiEI CGRYCG 3 cut(s) 49, 150, 154
BsiHKAI GWGCWC 1 cut(s) 485
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 3 cut(s) 288, 306, 445
BsiWI CGTACG 1 cut(s) 71
BslFI GGGAC 1 cut(s) 461
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 1 cut(s) 581
BsmBI CGTCTC 1 cut(s) 581
BsmFI GGGAC 1 cut(s) 461
BsnI GGCC 1 cut(s) 294
BsoBI CYCGRG 1 cut(s) 287
Bsp119I TTCGAA 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 485
Bsp143I GATC 7 cut(s) 42, 46, 61, 147, 151, 318, 570
BspACI CCGC 2 cut(s) 212, 295
BspANI GGCC 1 cut(s) 294
BspCNI CTCAG 1 cut(s) 105
BspDI ATCGAT 2 cut(s) 45, 150
BspLI GGNNCC 2 cut(s) 12, 293
BspT104I TTCGAA 1 cut(s) 429
BsrDI GCAATG 1 cut(s) 434
BsrI ACTGG 1 cut(s) 502
BssECI CCNNGG 4 cut(s) 114, 287, 288, 383
BssMI GATC 7 cut(s) 42, 46, 61, 147, 151, 318, 570
BssT1I CCWWGG 2 cut(s) 114, 383
Bst4CI ACNGT 1 cut(s) 37
BstBAI YACGTR 1 cut(s) 21
BstBI TTCGAA 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 2 cut(s) 92, 299
BstF5I GGATG 1 cut(s) 238
BstHHI GCGC 1 cut(s) 532
BstKTI GATC 7 cut(s) 45, 49, 64, 150, 154, 321, 573
BstMAI GTCTC 1 cut(s) 581
BstMBI GATC 7 cut(s) 42, 46, 61, 147, 151, 318, 570
BstMCI CGRYCG 3 cut(s) 49, 150, 154
BstMWI GCNNNNNNNGC 1 cut(s) 256
BstSCI CCNGG 4 cut(s) 286, 287, 304, 444
BstSLI GKGCMC 1 cut(s) 485
BstV1I GCAGC 3 cut(s) 108, 169, 270
BstV2I GAAGAC 1 cut(s) 149
BstXI CCANNNNNNTGG 1 cut(s) 516
Bsu15I ATCGAT 2 cut(s) 45, 150
BsuRI GGCC 1 cut(s) 294
BsuTUI ATCGAT 2 cut(s) 45, 150
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 238
Cac8I GCNNGC 1 cut(s) 552
CfoI GCGC 1 cut(s) 532
Cfr13I GGNCC 2 cut(s) 292, 596
Cfr9I CCCGGG 1 cut(s) 287
ClaI ATCGAT 2 cut(s) 45, 150
Csp6I GTAC 1 cut(s) 72
CviAII CATG 2 cut(s) 245, 411
CviJI RGCY 9 cut(s) 69, 99, 122, 143, 283, 294, 340, 352, 471
CviKI_1 RGCY 9 cut(s) 69, 99, 122, 143, 283, 294, 340, 352, 471
CviQI GTAC 1 cut(s) 72
DdeI CTNAG 2 cut(s) 92, 299
DpnI GATC 7 cut(s) 44, 48, 63, 149, 153, 320, 572
DpnII GATC 7 cut(s) 42, 46, 61, 147, 151, 318, 570
Eco130I CCWWGG 2 cut(s) 114, 383
Eco47I GGWCC 1 cut(s) 596
Eco72I CACGTG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 287
EcoT14I CCWWGG 2 cut(s) 114, 383
ErhI CCWWGG 2 cut(s) 114, 383
Esp3I CGTCTC 1 cut(s) 581
FaeI CATG 2 cut(s) 248, 414
FaiI YATR 9 cut(s) 83, 85, 180, 189, 246, 355, 412, 594, 602
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 1 cut(s) 461
FatI CATG 2 cut(s) 244, 410
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 4 cut(s) 97, 183, 284, 295
FokI GGATG 1 cut(s) 225
Fsp4HI GCNGC 4 cut(s) 97, 183, 284, 295
GlaI GCGC 1 cut(s) 531
GluI GCNGC 4 cut(s) 97, 183, 284, 295
HaeIII GGCC 1 cut(s) 294
HapII CCGG 3 cut(s) 288, 306, 445
HhaI GCGC 1 cut(s) 532
Hin1II CATG 2 cut(s) 248, 414
Hin6I GCGC 1 cut(s) 530
HinP1I GCGC 1 cut(s) 530
HinfI GANTC 3 cut(s) 311, 379, 431
HpaII CCGG 3 cut(s) 288, 306, 445
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 2 cut(s) 483, 636
Hpy188I TCNGA 4 cut(s) 27, 66, 147, 316
Hpy188III TCNNGA 3 cut(s) 40, 50, 617
Hpy8I GTNNAC 2 cut(s) 483, 636
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 4 cut(s) 20, 334, 495, 590
HpyCH4V TGCA 3 cut(s) 259, 273, 483
HpyF10VI GCNNNNNNNGC 1 cut(s) 256
HpyF3I CTNAG 2 cut(s) 92, 299
HpySE526I ACGT 4 cut(s) 20, 334, 495, 590
Hsp92II CATG 2 cut(s) 248, 414
HspAI GCGC 1 cut(s) 530
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 7 cut(s) 42, 46, 61, 147, 151, 318, 570
LmnI GCTCC 1 cut(s) 148
Lsp1109I GCAGC 3 cut(s) 108, 169, 270
LweI GCATC 1 cut(s) 612
MaeII ACGT 4 cut(s) 20, 334, 495, 590
MaeIII GTNAC 2 cut(s) 50, 504
MalI GATC 7 cut(s) 44, 48, 63, 149, 153, 320, 572
MboI GATC 7 cut(s) 42, 46, 61, 147, 151, 318, 570
MboII GAAGA 2 cut(s) 149, 209
MhlI GDGCHC 1 cut(s) 485
MluCI AATT 1 cut(s) 175
MmeI TCCRAC 1 cut(s) 497
MnlI CCTC 6 cut(s) 99, 189, 252, 339, 382, 411
MroXI GAANNNNTTC 2 cut(s) 123, 213
MseI TTAA 3 cut(s) 167, 279, 658
MslI CAYNNNNRTG 1 cut(s) 243
MspI CCGG 3 cut(s) 288, 306, 445
MspR9I CCNGG 4 cut(s) 288, 289, 306, 446
MwoI GCNNNNNNNGC 1 cut(s) 256
NciI CCSGG 4 cut(s) 288, 289, 306, 446
NdeII GATC 7 cut(s) 42, 46, 61, 147, 151, 318, 570
NlaIII CATG 2 cut(s) 248, 414
NlaIV GGNNCC 2 cut(s) 12, 293
NmuCI GTSAC 1 cut(s) 50
NspV TTCGAA 1 cut(s) 429
PdmI GAANNNNTTC 2 cut(s) 123, 213
PfeI GAWTC 3 cut(s) 311, 379, 431
Pfl23II CGTACG 1 cut(s) 71
PkrI GCNGC 4 cut(s) 98, 184, 285, 296
Ple19I CGATCG 3 cut(s) 49, 150, 154
PmaCI CACGTG 1 cut(s) 21
PmlI CACGTG 1 cut(s) 21
Ppu21I YACGTR 1 cut(s) 21
PspCI CACGTG 1 cut(s) 21
PspLI CGTACG 1 cut(s) 71
PspN4I GGNNCC 2 cut(s) 12, 293
PspPI GGNCC 2 cut(s) 292, 596
PvuI CGATCG 3 cut(s) 49, 150, 154
RsaI GTAC 1 cut(s) 73
RsaNI GTAC 1 cut(s) 72
RseI CAYNNNNRTG 1 cut(s) 243
SaqAI TTAA 3 cut(s) 167, 279, 658
SatI GCNGC 4 cut(s) 97, 183, 284, 295
Sau3AI GATC 7 cut(s) 42, 46, 61, 147, 151, 318, 570
Sau96I GGNCC 2 cut(s) 292, 596
ScrFI CCNGG 4 cut(s) 288, 289, 306, 446
SduI GDGCHC 1 cut(s) 485
SfaNI GCATC 1 cut(s) 612
SfuI TTCGAA 1 cut(s) 429
SinI GGWCC 1 cut(s) 596
SmaI CCCGGG 1 cut(s) 289
SmiMI CAYNNNNRTG 1 cut(s) 243
Sse9I AATT 1 cut(s) 175
SsiI CCGC 2 cut(s) 212, 295
StyD4I CCNGG 4 cut(s) 286, 287, 304, 444
StyI CCWWGG 2 cut(s) 114, 383
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 4 cut(s) 23, 337, 498, 593
TaqI TCGA 5 cut(s) 45, 138, 150, 344, 429
TasI AATT 1 cut(s) 175
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 3 cut(s) 311, 379, 431
Tru1I TTAA 3 cut(s) 167, 279, 658
Tru9I TTAA 3 cut(s) 167, 279, 658
TseFI GTSAC 1 cut(s) 50
TseI GCWGC 3 cut(s) 96, 182, 283
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 339
TspMI CCCGGG 1 cut(s) 287
VneI GTGCAC 1 cut(s) 481
VpaK11BI GGWCC 1 cut(s) 596
XmaI CCCGGG 1 cut(s) 287
XmnI GAANNNNTTC 2 cut(s) 123, 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.