Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0002701

Physical Location & Seq

Genomic Coordinates Reverse (-)

6147149 .. 6150576

Gene Structure 3428 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2832 bp

ATGGTTGAACAGTTAGCCATGAGTATAGTTTGGCTGCTGGTGTTGATGATTTTCTGTAGTGGGTTTGCCTCAAATGGTGCCAGCACCACCAATGTCTCCACTAGACCAGATGTTGTGAATCTTGGGGCGATTTTCTCTTTCGATACCTTTATTGGCAAAGTTGCCAAAGTGGCAATAGAAGCCGCAGTCGAAGATGTGAATTCTGATCCATCTGTTCTTGGTGGAACCAAGATGAGAGTTACAATGAAGGACTCAAATTACAGTGGACTTCTGGGCCTTATAGAAGCCTTACGGTTCATGGAGGAAGACACAATGGCGATAATTGGACCGCTGAATGCTGTCACGGCTCATATATTATCTAATATTGCAAATGAGCTCCTAGTTCCTCTAGTATCATTTTCGGTAACAGACCCCACGCTGTCAGCACTTCAGTTTCCTTTCTTTGTTAGAACTGCCCAAAACGATCTTTATCAAATGGCTGCGATAGCAGATATGGTTGATTACTACGGATGGAGGGAGGTAATTGCACTCTATGTTGACGATGACCATGGGAGAAATGGGATTACTGCATTATCAAATAAGCTTGCTGAGAAACGATGCAGGATATCATACAAGGCGCCATTGGTCCTTAATTCTAACCGGGATAGCATCACTGATGTACTGGTTAATGTGGCTTTAACAGAGTCTCGGATTATTGTTGTTCACGCTTATGACAGTTGGGGTTCTCTGGTGTTCGAAGTGGCCAAGTATCTTGGCATGATGGGAACTGGATATGTCTGGATAGCCACCAGTTGGCTTTCTACTTTAATAGATACCAACTCTCCCCTCCCTTCAGATACGATGGATGCTATGCAGGGAGTTCTAACATTGCGCATGTACACACCAGAGACAGAACTGAAGCGAAAATTTGTTTCAAGGTGGAGCAACTTGACTAGTGGACATAAAGGCCCAATTGGGTTGAATGCTTACGGTCTTTATGCCTACGATACTGTGTGGTTGCTTGCTCATGCAATTAATGCCTTTTTTGATCAAGGGGGAAACCTTTCATTCTCAAATGATTCAAGGTTGACTCAGCTTCGCGGAGGGGATCTAAATCTTGATGCTCTGAGCATCTTTAATGGAGGCAATCTGTTGTTGAAAAACATTTTGCAGGTTAATATGACTGGTGTATCTGGCCCGATAATGTTTACTCCACAAAAGGACCTCTTTCATCCTGCATTCGAGATCATAAATGTGATTGGAACGGGGATTAGGAAGGTTGGTTATTGGTCCAATTTTTCGGGATTATCAGTTGCGCCTCCAGAAACACTTTACACAAAGGCACCAAATCATTCTGATTCAAGTGATAAAGTGTACAGTGTGGTTTGGCCTGGACAAACAACGCAGAAGCCTCGTGGTTGGGTGTTTCCAAACAACGGAAGGCACTTGAGGTTTGGAGTCTCAAGGCGTGTTAGTTTTCGTGAATTCGTTTCATACACAGATGGCAATGACGCGTTCACTGGGTACTGCATAGATGTTTTTACTGCTGCAGTGAACTTGTTGCCCTATGCTGTCCCATACAAATTAATTCCTTTCGGTGATGGCCATAAGAATCCAAGTGCCACTGACCTTGTGCACAAAATCCAAACGGGTGAATTCGATGGAGCAATAAGTGACATTGCAATCACCACCAACCGAACAAGGATGGCGGATTTTACGCAGCCATATATCGAATCTGGGCTAGTAGTAGTTGCACCTGTTAAGCCAATATTGAATTCAAATCCTTGGGCTTTCCTGAGGCCATTCAGTCCAATGATGTGGGGTGTCACGGCCGCCTTCTTTCTCATAGTTGGAACAGTTGTTTGGATTTTAGAGCATAGGCAGAATGATGAATTTCGGGGGCCTCCTAAAAGACAACTCGTCACTATTCTGTGGTTTAGCTTTTCGACTTGGTTCTTTGCACATAGAGAAAATACAGTTAGCACCCTTGGTCGCTTAGTTCTAATTATATGGCTGTTTGTTGTTCTTATAATCAACTCAAGCTACACTGCAAGTTTGACCTCAATCCTTACAGTGCAAAAGCTTTCTTTCTCTATCAAAGGCCTTGACGATTTGCTTTCAAGCAACGATCCCATTGGCTACCAGCAGGGTTCATTTGCCCGGAATTATCTAATTGATGAACTCAACGTTGACGAGTCCAGACTCATTCCTCTTGTCACGCCAGATGACTATGCCAAAGCCTTGAAAGATGGTCCCCATAAAGGTGGTGTCACTGCAGTGATTGACGAGCGTGCATTCATTGAGCTATTCCTCTCAAGCAGATGTGAATTCAGTGTTGTGGGTCAAGAGTTCACCAAAACCAGTTGGGGATTTGCTTTTCCAAGGGACTCGCCTTTAGCGGTGGACATGTCTACTGCACTTCTGAAGCTGTCGGAGAACGGGGATCTACAAAGGATCTATGATAAATGGCTTATGAGAACTCCTTGTGCCTCAAATGGTGGAAAGCTGCAAGTCGACAGGCTTCAACTCAAAAGCTTCGGGGGCCTCTTTATTATTTGTGGTGCAGCTTGCTTCCTTGCACTCGCTATATATTTCTGTATTATGCTGCGCCAGTTCAGCAAGCACTACACCGAGGAACTGATCTCTACTGGTAGCTCCACGTCCGCACGCGTTCAAACATTTCTCACATTTGTTGATGAGAAGGAAGAGGAAGTGAAAACCCGGTCGAAAAGAAGACGAATGGAGAGGTTATCAAATAGAAGCGCGGAAGATGAAGCGATGTCTAACTCGAAAAGGAGACACCTTGATCAATCATCTTCAAGTCGGAGTTTTGATAATGGTAATCCTGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007267 GO:0008066 GO:0008150 GO:0008324 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009682 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009864 GO:0009867 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0031347 GO:0031349 GO:0032870 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0042742 GO:0043200 GO:0043207 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046873 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071395 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0080134 GO:0098542 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

944

Amino Acids

104.76

Weight (kDa)

6.35

Isoelectric Point (pI)

36.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	51 - 346	8.9e-13	Periplasmic binding protein
ANF_receptor	PF01094	56 - 414	2e-90	Receptor family ligand binding region
SBP_bac_3	PF00497	491 - 817	7.8e-12	Bacterial extracellular solute-binding proteins, family 3
Lig_chan-Glu_bd	PF10613	493 - 578	6.4e-09	Ligated ion channel L-glutamate- and glycine-binding site
Lig_chan	PF00060	598 - 850	1.7e-35	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002701)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g10191 FvH4_1g10191 FvH4_1g10191 FvH4_1g10191 FvH4_1g10191 FvH4_1g10191
malus_domestica	MD02G1114100.v1.1 MD15G1229200.v1.1
prunus_persica	Prupe.7G106900_v2.0.a1 Prupe.7G184200_v2.0.a1 Prupe.7G184200_v2.0.a1 Prupe.7G184200_v2.0.a1 Prupe.7G184200_v2.0.a1 Prupe.7G184200_v2.0.a1 Prupe.7G184600_v2.0.a1 Prupe.7G184600_v2.0.a1 Prupe.7G184600_v2.0.a1 Prupe.7G184600_v2.0.a1 Prupe.7G223100_v2.0.a1 Prupe.7G223100_v2.0.a1 Prupe.7G223100_v2.0.a1 Prupe.7G223100_v2.0.a1 Prupe.7G223100_v2.0.a1 Prupe.7G223100_v2.0.a1 Prupe.7G223100_v2.0.a1 Prupe.7G223100_v2.0.a1
pyrus_communis	pycom02g08900
rosa_chinensis	RchiOBHm_Chr2g0096991
rosa_laevigata	RLG00000016690
rosa_multiflora	Rmu_co8480363.1_g000001 Rmu_sc0005782.1_g000006
rosa_roxburghii	Rroxscaffold_2G00145100
rosa_rugosa	Rorug02G0062600 Rorug02G0062700
rosa_samantha	Rh2AG110500 Rh2BG112300 Rh2CG114400 Rh2DG113600
rosa_wichuraiana	Rw2G008550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2009
Acc16I	TGCGCA	1 cut(s)	872
Acc36I	ACCTGC	1 cut(s)	1141
AccB1I	GGYRCC	3 cut(s)	77, 616, 1321
AccB7I	CCANNNNNTGG	1 cut(s)	792
AccI	GTMKAC	2 cut(s)	2390, 2493
AccII	CGCG	4 cut(s)	1080, 1493, 2649, 2744
AciI	CCGC	8 cut(s)	183, 329, 1080, 1688, 1812, 2378, 2643, 2744
AclI	AACGTT	1 cut(s)	2166
AclWI	GGATC	5 cut(s)	200, 1095, 2102, 2430, 2441
AcoI	YGGCCR	3 cut(s)	741, 1582, 1809
AcsI	RAATTY	7 cut(s)	199, 905, 1463, 1634, 1753, 1871, 2306
AcuI	CTGAAG	4 cut(s)	413, 816, 917, 2423
AcyI	GRCGYC	1 cut(s)	617
AfaI	GTAC	4 cut(s)	660, 878, 1355, 1505
AfiI	CCNNNNNNNGG	3 cut(s)	792, 1415, 2240
AflIII	ACRYGT	3 cut(s)	1491, 2385, 2647
AhdI	GACNNNNNGTC	1 cut(s)	1898
AhlI	ACTAGT	1 cut(s)	932
AjiI	CACGTC	1 cut(s)	2640
AjnI	CCWGG	1 cut(s)	1369
AjuI	GAANNNNNNNTTGG	4 cut(s)	1738, 1770, 1825, 1857
AleI	CACNNNNGTG	1 cut(s)	2255
Alw21I	GWGCWC	2 cut(s)	378, 1617
Alw26I	GTCTC	5 cut(s)	100, 690, 881, 1444, 2770
Alw44I	GTGCAC	1 cut(s)	1613
AlwI	GGATC	5 cut(s)	200, 1095, 2102, 2430, 2441
ApaLI	GTGCAC	1 cut(s)	1613
ApeKI	GCWGC	7 cut(s)	34, 479, 1526, 1699, 2485, 2543, 2584
ApoI	RAATTY	7 cut(s)	199, 905, 1463, 1634, 1753, 1871, 2306
AseI	ATTAAT	2 cut(s)	1014, 1565
Asp700I	GAANNNNTTC	1 cut(s)	1042
AspLEI	GCGC	5 cut(s)	619, 873, 1297, 2589, 2744
AsuC2I	CCSGG	3 cut(s)	641, 2140, 2701
AsuHPI	GGTGA	4 cut(s)	1589, 1643, 1657, 2323
AsuII	TTCGAA	1 cut(s)	735
AvaII	GGWCC	5 cut(s)	326, 625, 1201, 1269, 2231
AxyI	CCTNAGG	1 cut(s)	1775
BaeGI	GKGCMC	1 cut(s)	1617
BaeI	ACNNNNGTAYC	2 cut(s)	1152, 1185
BalI	TGGCCA	2 cut(s)	743, 1584
BanI	GGYRCC	3 cut(s)	77, 616, 1321
BanII	GRGCYC	1 cut(s)	378
BauI	CACGAG	1 cut(s)	1392
BbsI	GAAGAC	2 cut(s)	312, 2719
Bbv12I	GWGCWC	2 cut(s)	378, 1617
BbvI	GCAGC	7 cut(s)	21, 466, 1513, 1711, 2472, 2555, 2571
BccI	CCATC	9 cut(s)	217, 504, 754, 835, 1475, 1574, 1634, 1678, 2222
BceAI	ACGGC	2 cut(s)	360, 1824
BcgI	CGANNNNNNTGC	2 cut(s)	1373, 1407
BciT130I	CCWGG	1 cut(s)	1371
BclI	TGATCA	2 cut(s)	1027, 2785
BcnI	CCSGG	3 cut(s)	641, 2140, 2701
BcoDI	GTCTC	5 cut(s)	100, 690, 881, 1444, 2770
BcuI	ACTAGT	1 cut(s)	932
BfaI	CTAG	5 cut(s)	102, 380, 389, 933, 1721
BfmI	CTRYAG	3 cut(s)	55, 1527, 2253
BfoI	RGCGCY	1 cut(s)	620
BfuAI	ACCTGC	1 cut(s)	1141
BglI	GCCNNNNNGGC	1 cut(s)	170
BisI	GCNGC	9 cut(s)	35, 183, 480, 1527, 1700, 1812, 2486, 2544, 2585
BlsI	GCNGC	9 cut(s)	36, 184, 481, 1528, 1701, 1813, 2487, 2545, 2586
Bme1390I	CCNGG	4 cut(s)	641, 1371, 2140, 2701
Bme18I	GGWCC	5 cut(s)	326, 625, 1201, 1269, 2231
BmeRI	GACNNNNNGTC	1 cut(s)	1898
BmgBI	CACGTC	1 cut(s)	2640
BmiI	GGNNCC	7 cut(s)	79, 226, 618, 1323, 1881, 2233, 2522
BmrFI	CCNGG	4 cut(s)	641, 1371, 2140, 2701
BmrI	ACTGGG	1 cut(s)	1509
BmsI	GCATC	5 cut(s)	587, 657, 835, 1090, 1119
BmuI	ACTGGG	1 cut(s)	1509
BpiI	GAAGAC	2 cut(s)	312, 2719
BpmI	CTGGAG	1 cut(s)	1284
Bpu14I	TTCGAA	1 cut(s)	735
BpuEI	CTTGAG	4 cut(s)	1426, 1447, 2002, 2278
BpuMI	CCSGG	3 cut(s)	641, 2140, 2701
BsaBI	GATNNNNATC	2 cut(s)	468, 1092
BsaHI	GRCGYC	1 cut(s)	617
BsaJI	CCNNGG	5 cut(s)	547, 1763, 1966, 2360, 2610
BsaXI	ACNNNNNCTCC	2 cut(s)	805, 835
Bsc4I	CCNNNNNNNGG	3 cut(s)	792, 1415, 2240
Bse1I	ACTGG	8 cut(s)	666, 772, 789, 1168, 1504, 2340, 2590, 2632
Bse21I	CCTNAGG	1 cut(s)	1775
Bse3DI	GCAATG	3 cut(s)	866, 1492, 1656
Bse8I	GATNNNNATC	2 cut(s)	468, 1092
BseBI	CCWGG	1 cut(s)	1371
BseDI	CCNNGG	5 cut(s)	547, 1763, 1966, 2360, 2610
BseGI	GGATG	4 cut(s)	515, 850, 1210, 1689
BseJI	GATNNNNATC	2 cut(s)	468, 1092
BseLI	CCNNNNNNNGG	3 cut(s)	792, 1415, 2240
BseMI	GCAATG	3 cut(s)	866, 1492, 1656
BseMII	CTCAG	4 cut(s)	579, 1085, 1097, 1766
BseNI	ACTGG	8 cut(s)	666, 772, 789, 1168, 1504, 2340, 2590, 2632
BseSI	GKGCMC	1 cut(s)	1617
BseX3I	CGGCCG	1 cut(s)	1809
BseXI	GCAGC	7 cut(s)	21, 466, 1513, 1711, 2472, 2555, 2571
BsgI	GTGCAG	2 cut(s)	2379, 2562
Bsh1236I	CGCG	4 cut(s)	1080, 1493, 2649, 2744
Bsh1285I	CGRYCG	2 cut(s)	1812, 2705
BshNI	GGYRCC	3 cut(s)	77, 616, 1321
BsiEI	CGRYCG	2 cut(s)	1812, 2705
BsiHKAI	GWGCWC	2 cut(s)	378, 1617
BsiSI	CCGG	3 cut(s)	640, 2140, 2701
BslFI	GGGAC	3 cut(s)	1538, 2217, 2378
BslI	CCNNNNNNNGG	3 cut(s)	792, 1415, 2240
BsmAI	GTCTC	5 cut(s)	100, 690, 881, 1444, 2770
BsmFI	GGGAC	3 cut(s)	1538, 2217, 2378
BsmI	GAATGC	4 cut(s)	340, 967, 1217, 2273
Bsp119I	TTCGAA	1 cut(s)	735
Bsp1286I	GDGCHC	2 cut(s)	378, 1617
Bsp1407I	TGTACA	2 cut(s)	876, 1353
Bsp19I	CCATGG	1 cut(s)	547
BspACI	CCGC	8 cut(s)	183, 329, 1080, 1688, 1812, 2378, 2643, 2744
BspCNI	CTCAG	4 cut(s)	580, 1084, 1098, 1767
BspFNI	CGCG	4 cut(s)	1080, 1493, 2649, 2744
BspLI	GGNNCC	7 cut(s)	79, 226, 618, 1323, 1881, 2233, 2522
BspMAI	CTGCAG	2 cut(s)	1531, 2257
BspMI	ACCTGC	1 cut(s)	1141
BspPI	GGATC	5 cut(s)	200, 1095, 2102, 2430, 2441
BspT104I	TTCGAA	1 cut(s)	735
BspT107I	GGYRCC	3 cut(s)	77, 616, 1321
BsrDI	GCAATG	3 cut(s)	866, 1492, 1656
BsrGI	TGTACA	2 cut(s)	876, 1353
BsrI	ACTGG	8 cut(s)	666, 772, 789, 1168, 1504, 2340, 2590, 2632
BssECI	CCNNGG	5 cut(s)	547, 1763, 1966, 2360, 2610
BssNI	GRCGYC	1 cut(s)	617
BssSI	CACGAG	1 cut(s)	1392
BssT1I	CCWWGG	4 cut(s)	547, 1763, 1966, 2360
Bst2BI	CACGAG	1 cut(s)	1392
Bst2UI	CCWGG	1 cut(s)	1371
Bst6I	CTCTTC	1 cut(s)	2679
BstACI	GRCGYC	1 cut(s)	617
BstAPI	GCANNNNNTGC	1 cut(s)	1016
BstAUI	TGTACA	2 cut(s)	876, 1353
BstBI	TTCGAA	1 cut(s)	735
BstC8I	GCNNGC	7 cut(s)	82, 585, 1002, 2271, 2548, 2600, 2647
BstDEI	CTNAG	5 cut(s)	588, 1071, 1106, 1775, 1975
BstDSI	CCRYGG	1 cut(s)	547
BstF5I	GGATG	4 cut(s)	515, 850, 1210, 1689
BstFNI	CGCG	4 cut(s)	1080, 1493, 2649, 2744
BstH2I	RGCGCY	1 cut(s)	620
BstHHI	GCGC	5 cut(s)	619, 873, 1297, 2589, 2744
BstMAI	GTCTC	5 cut(s)	100, 690, 881, 1444, 2770
BstMCI	CGRYCG	2 cut(s)	1812, 2705
BstMWI	GCNNNNNNNGC	7 cut(s)	170, 179, 344, 485, 1016, 2520, 2595
BstNI	CCWGG	1 cut(s)	1371
BstNSI	RCATGY	2 cut(s)	877, 2389
BstSCI	CCNGG	4 cut(s)	639, 1369, 2138, 2699
BstSFI	CTRYAG	3 cut(s)	55, 1527, 2253
BstSLI	GKGCMC	1 cut(s)	1617
BstUI	CGCG	4 cut(s)	1080, 1493, 2649, 2744
BstV1I	GCAGC	7 cut(s)	21, 466, 1513, 1711, 2472, 2555, 2571
BstV2I	GAAGAC	2 cut(s)	312, 2719
BstX2I	RGATCY	3 cut(s)	1087, 2422, 2433
BstXI	CCANNNNNNTGG	2 cut(s)	1797, 2243
BstYI	RGATCY	3 cut(s)	1087, 2422, 2433
BstZI	CGGCCG	1 cut(s)	1809
Bsu36I	CCTNAGG	1 cut(s)	1775
BtgI	CCRYGG	1 cut(s)	547
BtgZI	GCGATG	1 cut(s)	2771
BtrI	CACGTC	1 cut(s)	2640
BtsCI	GGATG	4 cut(s)	515, 850, 1210, 1689
BtsI	GCAGTG	4 cut(s)	1536, 2025, 2250, 2262
BveI	ACCTGC	1 cut(s)	1141
Cac8I	GCNNGC	7 cut(s)	82, 585, 1002, 2271, 2548, 2600, 2647
CfoI	GCGC	5 cut(s)	619, 873, 1297, 2589, 2744
CseI	GACGC	1 cut(s)	1499
Csp6I	GTAC	4 cut(s)	659, 877, 1354, 1504
CspCI	CAANNNNNGTGG	2 cut(s)	1591, 1626
CviAII	CATG	7 cut(s)	19, 298, 548, 757, 874, 1007, 2386
CviQI	GTAC	4 cut(s)	659, 877, 1354, 1504
DdeI	CTNAG	5 cut(s)	588, 1071, 1106, 1775, 1975
DinI	GGCGCC	1 cut(s)	618
DriI	GACNNNNNGTC	1 cut(s)	1898
EaeI	YGGCCR	3 cut(s)	741, 1582, 1809
EagI	CGGCCG	1 cut(s)	1809
Eam1104I	CTCTTC	1 cut(s)	2679
Eam1105I	GACNNNNNGTC	1 cut(s)	1898
EarI	CTCTTC	1 cut(s)	2679
EciI	GGCGGA	1 cut(s)	1703
Ecl136II	GAGCTC	1 cut(s)	376
EclXI	CGGCCG	1 cut(s)	1809
Eco130I	CCWWGG	4 cut(s)	547, 1763, 1966, 2360
Eco147I	AGGCCT	1 cut(s)	2082
Eco24I	GRGCYC	1 cut(s)	378
Eco32I	GATATC	1 cut(s)	606
Eco47I	GGWCC	5 cut(s)	326, 625, 1201, 1269, 2231
Eco52I	CGGCCG	1 cut(s)	1809
Eco53kI	GAGCTC	1 cut(s)	376
Eco57I	CTGAAG	4 cut(s)	413, 816, 917, 2423
Eco81I	CCTNAGG	1 cut(s)	1775
EcoICRI	GAGCTC	1 cut(s)	376
EcoO109I	RGGNCCY	3 cut(s)	1201, 1880, 2521
EcoRI	GAATTC	5 cut(s)	199, 1463, 1634, 1753, 2306
EcoRII	CCWGG	1 cut(s)	1369
EcoRV	GATATC	1 cut(s)	606
EcoT14I	CCWWGG	4 cut(s)	547, 1763, 1966, 2360
EcoT38I	GRGCYC	1 cut(s)	378
EgeI	GGCGCC	1 cut(s)	618
EheI	GGCGCC	1 cut(s)	618
ErhI	CCWWGG	4 cut(s)	547, 1763, 1966, 2360
FaeI	CATG	7 cut(s)	22, 301, 551, 760, 877, 1010, 2389
FalI	AAGNNNNNCTT	2 cut(s)	1190, 1222
FaqI	GGGAC	3 cut(s)	1538, 2217, 2378
FatI	CATG	7 cut(s)	18, 297, 547, 756, 873, 1006, 2385
FbaI	TGATCA	2 cut(s)	1027, 2785
FblI	GTMKAC	2 cut(s)	2390, 2493
Fnu4HI	GCNGC	9 cut(s)	35, 183, 480, 1527, 1700, 1812, 2486, 2544, 2585
FokI	GGATG	4 cut(s)	522, 857, 1197, 1696
FriOI	GRGCYC	1 cut(s)	378
Fsp4HI	GCNGC	9 cut(s)	35, 183, 480, 1527, 1700, 1812, 2486, 2544, 2585
FspBI	CTAG	5 cut(s)	102, 380, 389, 933, 1721
FspI	TGCGCA	1 cut(s)	872
GlaI	GCGC	5 cut(s)	618, 872, 1296, 2588, 2743
GluI	GCNGC	9 cut(s)	35, 183, 480, 1527, 1700, 1812, 2486, 2544, 2585
GsuI	CTGGAG	1 cut(s)	1284
HaeII	RGCGCY	1 cut(s)	620
HapII	CCGG	3 cut(s)	640, 2140, 2701
HgaI	GACGC	1 cut(s)	1499
HhaI	GCGC	5 cut(s)	619, 873, 1297, 2589, 2744
Hin1I	GRCGYC	1 cut(s)	617
Hin1II	CATG	7 cut(s)	22, 301, 551, 760, 877, 1010, 2389
Hin6I	GCGC	5 cut(s)	617, 871, 1295, 2587, 2742
HinP1I	GCGC	5 cut(s)	617, 871, 1295, 2587, 2742
HincII	GTYRAC	4 cut(s)	538, 1068, 2170, 2494
HindII	GTYRAC	4 cut(s)	538, 1068, 2170, 2494
HindIII	AAGCTT	3 cut(s)	581, 2060, 2512
HpaII	CCGG	3 cut(s)	640, 2140, 2701
HphI	GGTGA	4 cut(s)	1589, 1643, 1657, 2323
Hpy188I	TCNGA	8 cut(s)	205, 690, 835, 1107, 1336, 2403, 2413, 2805
Hpy188III	TCNNGA	9 cut(s)	778, 1097, 1222, 1281, 1301, 1460, 1774, 2178, 2324
HpyAV	CCTTC	6 cut(s)	241, 840, 1249, 1413, 1825, 2674
HpyCH4IV	ACGT	2 cut(s)	2166, 2639
HpyF10VI	GCNNNNNNNGC	7 cut(s)	170, 179, 344, 485, 1016, 2520, 2595
HpyF3I	CTNAG	5 cut(s)	588, 1071, 1106, 1775, 1975
HpySE526I	ACGT	2 cut(s)	2166, 2639
Hsp92I	GRCGYC	1 cut(s)	617
Hsp92II	CATG	7 cut(s)	22, 301, 551, 760, 877, 1010, 2389
HspAI	GCGC	5 cut(s)	617, 871, 1295, 2587, 2742
KasI	GGCGCC	1 cut(s)	616
Ksp22I	TGATCA	2 cut(s)	1027, 2785
LmnI	GCTCC	4 cut(s)	381, 921, 1643, 2639
Lsp1109I	GCAGC	7 cut(s)	21, 466, 1513, 1711, 2472, 2555, 2571
LweI	GCATC	5 cut(s)	587, 657, 835, 1090, 1119
MaeI	CTAG	5 cut(s)	102, 380, 389, 933, 1721
MaeII	ACGT	2 cut(s)	2166, 2639
MaeIII	GTNAC	8 cut(s)	238, 340, 403, 1652, 1804, 1900, 2194, 2248
MboII	GAAGA	6 cut(s)	203, 317, 2696, 2724, 2759, 2787
MfeI	CAATTG	1 cut(s)	951
MflI	RGATCY	3 cut(s)	1087, 2422, 2433
MhlI	GDGCHC	2 cut(s)	378, 1617
MlsI	TGGCCA	2 cut(s)	743, 1584
MluI	ACGCGT	2 cut(s)	1491, 2647
MluNI	TGGCCA	2 cut(s)	743, 1584
Mly113I	GGCGCC	1 cut(s)	617
MlyI	GAGTC	7 cut(s)	245, 692, 1063, 1446, 2175, 2183, 2360
MmeI	TCCRAC	3 cut(s)	1810, 2391, 2783
Mox20I	TGGCCA	2 cut(s)	743, 1584
MroXI	GAANNNNTTC	1 cut(s)	1042
MscI	TGGCCA	2 cut(s)	743, 1584
MseI	TTAA	9 cut(s)	630, 666, 677, 806, 1014, 1116, 1155, 1565, 1740
MslI	CAYNNNNRTG	4 cut(s)	708, 1232, 2241, 2255
Msp20I	TGGCCA	2 cut(s)	743, 1584
MspA1I	CMGCKG	1 cut(s)	331
MspI	CCGG	3 cut(s)	640, 2140, 2701
MspR9I	CCNGG	4 cut(s)	641, 1371, 2140, 2701
MunI	CAATTG	1 cut(s)	951
Mva1269I	GAATGC	4 cut(s)	340, 967, 1217, 2273
MvaI	CCWGG	1 cut(s)	1371
MvnI	CGCG	4 cut(s)	1080, 1493, 2649, 2744
MwoI	GCNNNNNNNGC	7 cut(s)	170, 179, 344, 485, 1016, 2520, 2595
NarI	GGCGCC	1 cut(s)	617
NciI	CCSGG	3 cut(s)	641, 2140, 2701
NcoI	CCATGG	1 cut(s)	547
NlaIII	CATG	7 cut(s)	22, 301, 551, 760, 877, 1010, 2389
NlaIV	GGNNCC	7 cut(s)	79, 226, 618, 1323, 1881, 2233, 2522
NmuCI	GTSAC	6 cut(s)	340, 1652, 1804, 1900, 2194, 2248
NsbI	TGCGCA	1 cut(s)	872
NspI	RCATGY	2 cut(s)	877, 2389
NspV	TTCGAA	1 cut(s)	735
OliI	CACNNNNGTG	1 cut(s)	2255
PceI	AGGCCT	1 cut(s)	2082
PciI	ACATGT	1 cut(s)	2385
PctI	GAATGC	4 cut(s)	340, 967, 1217, 2273
PdmI	GAANNNNTTC	1 cut(s)	1042
PfeI	GAWTC	5 cut(s)	118, 1058, 1337, 1591, 1712
PflMI	CCANNNNNTGG	1 cut(s)	792
PkrI	GCNGC	9 cut(s)	36, 184, 481, 1528, 1701, 1813, 2487, 2545, 2586
PleI	GAGTC	7 cut(s)	245, 691, 1063, 1445, 2175, 2182, 2360
PluTI	GGCGCC	1 cut(s)	620
PpsI	GAGTC	7 cut(s)	245, 691, 1063, 1445, 2175, 2182, 2360
PpuMI	RGGWCCY	1 cut(s)	1201
PscI	ACATGT	1 cut(s)	2385
PshBI	ATTAAT	2 cut(s)	1014, 1565
PsiI	TTATAA	1 cut(s)	2009
Psp124BI	GAGCTC	1 cut(s)	378
Psp1406I	AACGTT	1 cut(s)	2166
Psp5II	RGGWCCY	1 cut(s)	1201
Psp6I	CCWGG	1 cut(s)	1369
PspGI	CCWGG	1 cut(s)	1369
PspN4I	GGNNCC	7 cut(s)	79, 226, 618, 1323, 1881, 2233, 2522
PspPPI	RGGWCCY	1 cut(s)	1201
PstI	CTGCAG	2 cut(s)	1531, 2257
PsuI	RGATCY	3 cut(s)	1087, 2422, 2433
RsaI	GTAC	4 cut(s)	660, 878, 1355, 1505
RsaNI	GTAC	4 cut(s)	659, 877, 1354, 1504
RseI	CAYNNNNRTG	4 cut(s)	708, 1232, 2241, 2255
SacI	GAGCTC	1 cut(s)	378
SalI	GTCGAC	1 cut(s)	2492
SaqAI	TTAA	9 cut(s)	630, 666, 677, 806, 1014, 1116, 1155, 1565, 1740
SatI	GCNGC	9 cut(s)	35, 183, 480, 1527, 1700, 1812, 2486, 2544, 2585
SchI	GAGTC	7 cut(s)	245, 692, 1063, 1446, 2175, 2183, 2360
ScrFI	CCNGG	4 cut(s)	641, 1371, 2140, 2701
SduI	GDGCHC	2 cut(s)	378, 1617
SfaNI	GCATC	5 cut(s)	587, 657, 835, 1090, 1119
SfcI	CTRYAG	3 cut(s)	55, 1527, 2253
SfoI	GGCGCC	1 cut(s)	618
SfuI	TTCGAA	1 cut(s)	735
SinI	GGWCC	5 cut(s)	326, 625, 1201, 1269, 2231
SmiMI	CAYNNNNRTG	4 cut(s)	708, 1232, 2241, 2255
SmlI	CTYRAG	4 cut(s)	1426, 1441, 2017, 2293
SmoI	CTYRAG	4 cut(s)	1426, 1441, 2017, 2293
SpeI	ACTAGT	1 cut(s)	932
SseBI	AGGCCT	1 cut(s)	2082
SsiI	CCGC	8 cut(s)	183, 329, 1080, 1688, 1812, 2378, 2643, 2744
SspDI	GGCGCC	1 cut(s)	616
SspI	AATATT	2 cut(s)	364, 1749
SspMI	CTAG	5 cut(s)	102, 380, 389, 933, 1721
SstI	GAGCTC	1 cut(s)	378
StuI	AGGCCT	1 cut(s)	2082
StyD4I	CCNGG	4 cut(s)	639, 1369, 2138, 2699
StyI	CCWWGG	4 cut(s)	547, 1763, 1966, 2360
TaiI	ACGT	2 cut(s)	2169, 2642
TatI	WGTACW	3 cut(s)	658, 876, 1353
TauI	GCSGC	2 cut(s)	185, 1814
TfiI	GAWTC	5 cut(s)	118, 1058, 1337, 1591, 1712
Tru1I	TTAA	9 cut(s)	630, 666, 677, 806, 1014, 1116, 1155, 1565, 1740
Tru9I	TTAA	9 cut(s)	630, 666, 677, 806, 1014, 1116, 1155, 1565, 1740
TseFI	GTSAC	6 cut(s)	340, 1652, 1804, 1900, 2194, 2248
TseI	GCWGC	7 cut(s)	34, 479, 1526, 1699, 2485, 2543, 2584
Tsp45I	GTSAC	6 cut(s)	340, 1652, 1804, 1900, 2194, 2248
TspGWI	ACGGA	2 cut(s)	522, 1431
Van91I	CCANNNNNTGG	1 cut(s)	792
VneI	GTGCAC	1 cut(s)	1613
VpaK11BI	GGWCC	5 cut(s)	326, 625, 1201, 1269, 2231
VspI	ATTAAT	2 cut(s)	1014, 1565
XapI	RAATTY	7 cut(s)	199, 905, 1463, 1634, 1753, 1871, 2306
XceI	RCATGY	2 cut(s)	877, 2389
XcmI	CCANNNNNNNNNTGG	1 cut(s)	554
XmiI	GTMKAC	2 cut(s)	2390, 2493
XmnI	GAANNNNTTC	1 cut(s)	1042
XspI	CTAG	5 cut(s)	102, 380, 389, 933, 1721

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.