Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0002701

Physical Location & Seq

Genomic Coordinates Reverse (-)

9726039 .. 9730693

Gene Structure 4655 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ47187

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2829 bp

ATGAGTGGACAATTCACCATGAATATAGCTTGGTTCCTGCTTTTGATGGTTTTCTTTAATGGGTTTGCCTCACATGGAGCTACCACCAACATCTCTACAAGACCCCAATTTGTAAATGTTGGGGCCATGCTTGCTCTCAATTCCATTGTTGGCAAAGTTGCCAAAGTGGCAATACAAGCTGCTGTTGAAGATGTGAATTCTGACCCTTCTGTTCTCAGTGGAACCAAGATGATAGTTACAACGCAGAATTCAAACTACAGTGGATTTCTGGGCATAATTGAGGCTTTACGATTCATGGAGAAGGACACAGTGGCCATAATTGGACCCCAGAATGCTGTAACGGCTCATGCAGTATCTCACATTGCAAATGAGCTGCACACTCCTCTGTTGTCATTTGCAGTAACAGATCCCACCCTGTCTTCACTTCAGTTCCCATATTTTGTTAGGACTACTCAGAACGATTTGTTTCAGATGGCTGCAGTAGCTGATATGGTTGAGTATTATGGATGGAGAGAGGTAATTGCAATATATGTTGATGATGATTTAGGGAGAAATGGGATTGCAGCATTAGGGGATTTGCTTGCTGAGAAACGTTCTAAGATCTCATACAAAGCACCTCTGGTTCTTGATCCTACTCGGAATAACATCACTGATGTACTGATTAAAGTGTCTTTAACCGAGTCTAGGATTATTGTTCTTCATGTTTATCCAGGTTGGGGCCCTGAGGTGCTTAGTGTGGCCAATTATCTTGGCATGATGGGAACAGGGTTTGTTTGGATAGCTACTCATTGGCTTTCTAGTTATATAGATTCCACTATGCCCCTCCCTATGAGTACTTTGGATAACATGCAAGGAGTTCTAACATTGCGCATGTACACGCCAGATACAGAACAGAAAAGAAAATTTGTTTCGAGGTGGAGCAACTTGACTGGTGGTAACCAAATTGGGTTGAATGCTTTCGGTCTCTATGCTTATGACTCTGTATGGTTGCTTGCTCATGCACTTGATGCCTTTTTTGATCAAGGGGGAAACATTTCATTCTCAAATGATTCAAGGTTGACTCAGATGCCCAGAGGGGACCTAAATCTTGAATATCTGAGTATATTTGATGGAGGGAGCTTGTTACTTCGTAACATATTTGGAGTTAATATGACTGGCACAACTGGCTCGTTTAAGTATACTCCAGATAGGGACCTCATTCGTCCTGCTTATGAAATCATAAATGTGATTGGCACTGGGGTTAGGAGGATTGGTTATTGGTCTAACCATTCTGGTTTATCAGTTAAGCCTCCAGAAACATTTTACACAAGCCCACCAAATCATTCTAGTTCAAACCAAAGCCTTTACAGTGTGATTTGGCCTGGAGAGACAGCACAGCAACCTCGTGGTTGGGTTTTTCCAAACAATGGGAGGCATTTGAAGATTGCAGTCCCAAACCGTGCTAGTTTCCGTGAATTTGCATCATATACACGAAGCAATGACATGTTCACTGGGTACTGCATTGATGTTTTTACTGCTGCATTGAACTTGTTGCCCTATGCTGTTCCCTTCAAATTTTTTCCTGTTGGCGATGGTAAAAGAAATCCAAGGAGCACTGACCTTGTGCACACAATCCAAACGGGTGAATATGATGCAGTAGTAGGTGATATTGCAATCACCACCAACAGAACCAGGATGGCAGATTTTACGCAGCCATACATCGAATCTGGGCTAGTAGTAGTTGCACCTGTTAAGACGACATTGAATTCAAATCCTTGGGCATTTCTGAGGCCATTCAATAAAATGATGTGGGCTGTCACTGCTGCCTTCTTTCTCATTGTTGGAACAGTTGTTTGGATATTAGAGCATAGACTGAATGATGAATTTCGGGGCCCTCCTAGAAGACAACTCGTCACTATTCTTTGGTTTAGCTTTTCAACTTGGTTCTTTGCCCATAGAGAAAATACAGTCAGCACCCTGGGTCGCCTAGTACTAATCATATGGCTATTTGTGGTTCTTATCATCAACTCAAGCTATACTGCAAGTTTGACCTCAATCCTAACGGTGCAAAAGCTTTCTTCCTCCATCAAAGGCATTGAAACTTTGCTTGAAAGCAAGGACCCCATTGGTTACCAACAGGGTTCATTTGCCAAGTATTATCTCGTTGAGGAACTCCACATTGACAGTTCCAGACTCGTGCCACTCGTCATGCCAGAAGATTATGTGAGAGCCTTAAAAGCTGGTCCACATAAGGAAGGTGGTGTTGCCGCAGTGATTGATGAGCGTGCATACATGGAGCTATTCCTCTCAAGCCGATGCGATTTCAGCATTGTAGGTCAAGAATTTACCAAAACCGGATGGGGATTTGCTTTCCCAAGGGACTCGCCTCTATCAGTGGACCTGTCAACTGCTCTGTTGAAGCTGTCGGATAATGGGGATCTACAGAGGATCCATGACAAATGGCTTCTCAGAAGTCCTTGTACTTCACCAGGAGCAAAGCTAGAAGTGGACAGGCTTGAACTTAGAAGCTTCTCGGCCCTTTTTATTATCTGTGGCGGAGCTTGTTTGGTTGCTCTCATTATATATTTCAGCATGATGTGTCACCAGTTCACCAAGCGGTACACTGACAGTTTGTCTTCTTCCGGGAGCTCCACATCTCGGCGTCTTCAGACCTTTCTCACATTTGTTGACGAGAAGGAAGAAGTACAAAGCCGATCCAAAAGGAGGAGCATGGAGAAGATGTCAAACCGAAGTGCGGGTGAAGATGACTCGTCAAACAGTTCCAAAAGAAGACATATAGATCAGTACTCATGTGCAAGCAGAAGTTTTGAAGACAGTAACAATGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007267 GO:0008066 GO:0008150 GO:0008324 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009682 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009864 GO:0009867 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0031347 GO:0031349 GO:0032870 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0042742 GO:0043200 GO:0043207 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046873 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071395 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0080134 GO:0098542 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

942

Amino Acids

105.11

Weight (kDa)

7.29

Isoelectric Point (pI)

46.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	48 - 337	1.4e-19	Periplasmic binding protein
ANF_receptor	PF01094	55 - 411	1e-86	Receptor family ligand binding region
SBP_bac_3	PF00497	489 - 815	7.7e-11	Bacterial extracellular solute-binding proteins, family 3
Lig_chan-Glu_bd	PF10613	489 - 576	2.8e-08	Ligated ion channel L-glutamate- and glycine-binding site
Lig_chan	PF00060	595 - 848	2.9e-34	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002701)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g10191 FvH4_1g10191 FvH4_1g10191 FvH4_1g10191 FvH4_1g10191 FvH4_1g10191
malus_domestica	MD02G1114100.v1.1 MD15G1229200.v1.1
prunus_persica	Prupe.7G106900_v2.0.a1 Prupe.7G184200_v2.0.a1 Prupe.7G184200_v2.0.a1 Prupe.7G184200_v2.0.a1 Prupe.7G184200_v2.0.a1 Prupe.7G184200_v2.0.a1 Prupe.7G184600_v2.0.a1 Prupe.7G184600_v2.0.a1 Prupe.7G184600_v2.0.a1 Prupe.7G184600_v2.0.a1 Prupe.7G223100_v2.0.a1 Prupe.7G223100_v2.0.a1 Prupe.7G223100_v2.0.a1 Prupe.7G223100_v2.0.a1 Prupe.7G223100_v2.0.a1 Prupe.7G223100_v2.0.a1 Prupe.7G223100_v2.0.a1 Prupe.7G223100_v2.0.a1
pyrus_communis	pycom02g08900
rosa_chinensis	RchiOBHm_Chr2g0096991
rosa_laevigata	RLG00000016690
rosa_multiflora	Rmu_co8480363.1_g000001 Rmu_sc0005782.1_g000006
rosa_roxburghii	Rroxscaffold_2G00145100
rosa_rugosa	Rorug02G0062600 Rorug02G0062700
rosa_samantha	Rh2AG110500 Rh2BG112300 Rh2CG114400 Rh2DG113600
rosa_wichuraiana	Rw2G008550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	869
AccB7I	CCANNNNNTGG	1 cut(s)	1406
AccI	GTMKAC	1 cut(s)	1178
AciI	CCGC	4 cut(s)	2247, 2535, 2596, 2735
AclI	AACGTT	1 cut(s)	592
AclWI	GGATC	6 cut(s)	401, 623, 2422, 2424, 2435, 2688
AcoI	YGGCCR	2 cut(s)	312, 738
AcsI	RAATTY	8 cut(s)	196, 247, 902, 1454, 1553, 1744, 1862, 2321
AcuI	CTGAAG	2 cut(s)	410, 2630
AcyI	GRCGYC	1 cut(s)	2641
AfaI	GTAC	9 cut(s)	657, 835, 875, 1496, 1971, 2461, 2600, 2685, 2786
AfiI	CCNNNNNNNGG	6 cut(s)	684, 716, 1406, 2637, 2703, 2734
AflIII	ACRYGT	1 cut(s)	1482
AhdI	GACNNNNNGTC	2 cut(s)	1889, 2611
AjnI	CCWGG	5 cut(s)	709, 1360, 1670, 1956, 2467
AjuI	GAANNNNNNNTTGG	2 cut(s)	1816, 1848
Alw21I	GWGCWC	3 cut(s)	1595, 1608, 2630
Alw26I	GTCTC	2 cut(s)	968, 1361
Alw44I	GTGCAC	1 cut(s)	1604
AlwI	GGATC	6 cut(s)	401, 623, 2422, 2424, 2435, 2688
AlwNI	CAGNNNCTG	1 cut(s)	485
AoxI	GGCC	8 cut(s)	123, 312, 718, 738, 1358, 1769, 1870, 2514
ApaI	GGGCCC	2 cut(s)	722, 1874
ApaLI	GTGCAC	1 cut(s)	1604
ApeKI	GCWGC	7 cut(s)	179, 373, 476, 563, 1517, 1690, 1802
ApoI	RAATTY	8 cut(s)	196, 247, 902, 1454, 1553, 1744, 1862, 2321
Asp700I	GAANNNNTTC	2 cut(s)	956, 1033
AspLEI	GCGC	1 cut(s)	870
AsuC2I	CCSGG	1 cut(s)	2623
AsuHPI	GGTGA	8 cut(s)	7, 1634, 1648, 1655, 2457, 2573, 2581, 2750
AvaII	GGWCC	6 cut(s)	323, 1078, 1192, 2098, 2222, 2377
AxyI	CCTNAGG	1 cut(s)	723
BaeGI	GKGCMC	3 cut(s)	722, 1608, 1874
BalI	TGGCCA	2 cut(s)	314, 740
BamHI	GGATCC	1 cut(s)	2427
BanII	GRGCYC	3 cut(s)	722, 1874, 2630
BarI	GAAGNNNNNNTAC	2 cut(s)	2443, 2475
BauI	CACGAG	2 cut(s)	1383, 2174
BbsI	GAAGAC	6 cut(s)	411, 1888, 2607, 2636, 2776, 2817
Bbv12I	GWGCWC	3 cut(s)	1595, 1608, 2630
BbvI	GCAGC	7 cut(s)	166, 360, 463, 575, 1504, 1702, 1789
BccI	CCATC	9 cut(s)	40, 466, 501, 751, 1103, 1565, 1669, 2072, 2331
BceAI	ACGGC	1 cut(s)	357
BciT130I	CCWGG	5 cut(s)	711, 1362, 1672, 1958, 2469
BclI	TGATCA	1 cut(s)	1018
BcnI	CCSGG	1 cut(s)	2623
BcoDI	GTCTC	2 cut(s)	968, 1361
BfaI	CTAG	8 cut(s)	684, 798, 1326, 1443, 1712, 1878, 1967, 2480
BfmI	CTRYAG	3 cut(s)	256, 477, 2420
BglI	GCCNNNNNGGC	1 cut(s)	167
BglII	AGATCT	1 cut(s)	600
BisI	GCNGC	8 cut(s)	180, 374, 477, 564, 1518, 1691, 1803, 2247
BlsI	GCNGC	8 cut(s)	181, 375, 478, 565, 1519, 1692, 1804, 2248
BmcAI	AGTACT	3 cut(s)	835, 1971, 2786
Bme1390I	CCNGG	6 cut(s)	711, 1362, 1672, 1958, 2469, 2623
Bme18I	GGWCC	6 cut(s)	323, 1078, 1192, 2098, 2222, 2377
BmeRI	GACNNNNNGTC	2 cut(s)	1889, 2611
BmrFI	CCNGG	6 cut(s)	711, 1362, 1672, 1958, 2469, 2623
BmrI	ACTGGG	2 cut(s)	1245, 1500
BmsI	GCATC	5 cut(s)	997, 1056, 1469, 1621, 2285
BmuI	ACTGGG	2 cut(s)	1245, 1500
BpiI	GAAGAC	6 cut(s)	411, 1888, 2607, 2636, 2776, 2817
BpmI	CTGGAG	3 cut(s)	1167, 1275, 1383
BpuEI	CTTGAG	2 cut(s)	1993, 2272
BpuMI	CCSGG	1 cut(s)	2623
BsaHI	GRCGYC	1 cut(s)	2641
BsaI	GGTCTC	1 cut(s)	968
BsaJI	CCNNGG	5 cut(s)	1586, 1754, 1956, 1957, 2354
BsaWI	WCCGGW	1 cut(s)	2333
BsaXI	ACNNNNNCTCC	4 cut(s)	1237, 1267, 2705, 2735
Bsc4I	CCNNNNNNNGG	6 cut(s)	684, 716, 1406, 2637, 2703, 2734
Bse1I	ACTGG	6 cut(s)	934, 1159, 1168, 1240, 1495, 2584
Bse21I	CCTNAGG	1 cut(s)	723
Bse3DI	GCAATG	3 cut(s)	360, 863, 1483
BseBI	CCWGG	5 cut(s)	711, 1362, 1672, 1958, 2469
BseDI	CCNNGG	5 cut(s)	1586, 1754, 1956, 1957, 2354
BseGI	GGATG	3 cut(s)	512, 1680, 2342
BseLI	CCNNNNNNNGG	6 cut(s)	684, 716, 1406, 2637, 2703, 2734
BseMI	GCAATG	3 cut(s)	360, 863, 1483
BseMII	CTCAG	8 cut(s)	229, 467, 576, 714, 1076, 1088, 1757, 2461
BseNI	ACTGG	6 cut(s)	934, 1159, 1168, 1240, 1495, 2584
BseRI	GAGGAG	2 cut(s)	372, 2719
BseSI	GKGCMC	3 cut(s)	722, 1608, 1874
BseXI	GCAGC	7 cut(s)	166, 360, 463, 575, 1504, 1702, 1789
BsgI	GTGCAG	1 cut(s)	359
BshFI	GGCC	8 cut(s)	125, 314, 720, 740, 1360, 1771, 1872, 2516
BsiHKAI	GWGCWC	3 cut(s)	1595, 1608, 2630
BsiSI	CCGG	2 cut(s)	2334, 2622
BslFI	GGGAC	4 cut(s)	1091, 1205, 1415, 2372
BslI	CCNNNNNNNGG	6 cut(s)	684, 716, 1406, 2637, 2703, 2734
BsmAI	GTCTC	2 cut(s)	968, 1361
BsmFI	GGGAC	4 cut(s)	1091, 1205, 1415, 2372
BsmI	GAATGC	2 cut(s)	337, 958
BsnI	GGCC	8 cut(s)	125, 314, 720, 740, 1360, 1771, 1872, 2516
Bso31I	GGTCTC	1 cut(s)	968
Bsp120I	GGGCCC	2 cut(s)	718, 1870
Bsp1286I	GDGCHC	5 cut(s)	722, 1595, 1608, 1874, 2630
Bsp1407I	TGTACA	1 cut(s)	873
Bsp143I	GATC	8 cut(s)	406, 600, 628, 1018, 2416, 2427, 2693, 2779
BspACI	CCGC	4 cut(s)	2247, 2535, 2596, 2735
BspANI	GGCC	8 cut(s)	125, 314, 720, 740, 1360, 1771, 1872, 2516
BspCNI	CTCAG	8 cut(s)	228, 466, 577, 715, 1075, 1089, 1758, 2460
BspMAI	CTGCAG	1 cut(s)	481
BspPI	GGATC	6 cut(s)	401, 623, 2422, 2424, 2435, 2688
BspTNI	GGTCTC	1 cut(s)	968
BsrDI	GCAATG	3 cut(s)	360, 863, 1483
BsrGI	TGTACA	1 cut(s)	873
BsrI	ACTGG	6 cut(s)	934, 1159, 1168, 1240, 1495, 2584
BssECI	CCNNGG	5 cut(s)	1586, 1754, 1956, 1957, 2354
BssMI	GATC	8 cut(s)	406, 600, 628, 1018, 2416, 2427, 2693, 2779
BssNAI	GTATAC	1 cut(s)	1179
BssNI	GRCGYC	1 cut(s)	2641
BssSI	CACGAG	2 cut(s)	1383, 2174
BssT1I	CCWWGG	3 cut(s)	1586, 1754, 2354
Bst1107I	GTATAC	1 cut(s)	1179
Bst2BI	CACGAG	2 cut(s)	1383, 2174
Bst2UI	CCWGG	5 cut(s)	711, 1362, 1672, 1958, 2469
BstACI	GRCGYC	1 cut(s)	2641
BstAPI	GCANNNNNTGC	1 cut(s)	1007
BstAUI	TGTACA	1 cut(s)	873
BstC8I	GCNNGC	5 cut(s)	132, 582, 993, 2265, 2797
BstEII	GGTNACC	2 cut(s)	935, 2108
BstF5I	GGATG	3 cut(s)	512, 1680, 2342
BstHHI	GCGC	1 cut(s)	870
BstKTI	GATC	8 cut(s)	409, 603, 631, 1021, 2419, 2430, 2696, 2782
BstMAI	GTCTC	2 cut(s)	968, 1361
BstMBI	GATC	8 cut(s)	406, 600, 628, 1018, 2416, 2427, 2693, 2779
BstNI	CCWGG	5 cut(s)	711, 1362, 1672, 1958, 2469
BstNSI	RCATGY	3 cut(s)	850, 874, 1486
BstPI	GGTNACC	2 cut(s)	935, 2108
BstSCI	CCNGG	6 cut(s)	709, 1360, 1670, 1956, 2467, 2621
BstSFI	CTRYAG	3 cut(s)	256, 477, 2420
BstSLI	GKGCMC	3 cut(s)	722, 1608, 1874
BstV1I	GCAGC	7 cut(s)	166, 360, 463, 575, 1504, 1702, 1789
BstV2I	GAAGAC	6 cut(s)	411, 1888, 2607, 2636, 2776, 2817
BstX2I	RGATCY	4 cut(s)	406, 600, 2416, 2427
BstYI	RGATCY	4 cut(s)	406, 600, 2416, 2427
BstZ17I	GTATAC	1 cut(s)	1179
Bsu36I	CCTNAGG	1 cut(s)	723
BsuRI	GGCC	8 cut(s)	125, 314, 720, 740, 1360, 1771, 1872, 2516
BtgZI	GCGATG	1 cut(s)	1584
BtsCI	GGATG	3 cut(s)	512, 1680, 2342
BtsI	GCAGTG	2 cut(s)	1797, 2256
Cac8I	GCNNGC	5 cut(s)	132, 582, 993, 2265, 2797
CaiI	CAGNNNCTG	1 cut(s)	485
CfoI	GCGC	1 cut(s)	870
CseI	GACGC	1 cut(s)	2630
Csp6I	GTAC	9 cut(s)	656, 834, 874, 1495, 1970, 2460, 2599, 2684, 2785
CviQI	GTAC	9 cut(s)	656, 834, 874, 1495, 1970, 2460, 2599, 2684, 2785
DpnI	GATC	8 cut(s)	408, 602, 630, 1020, 2418, 2429, 2695, 2781
DpnII	GATC	8 cut(s)	406, 600, 628, 1018, 2416, 2427, 2693, 2779
DriI	GACNNNNNGTC	2 cut(s)	1889, 2611
EaeI	YGGCCR	2 cut(s)	312, 738
Eam1105I	GACNNNNNGTC	2 cut(s)	1889, 2611
EciI	GGCGGA	1 cut(s)	2550
Ecl136II	GAGCTC	1 cut(s)	2628
Eco130I	CCWWGG	3 cut(s)	1586, 1754, 2354
Eco24I	GRGCYC	3 cut(s)	722, 1874, 2630
Eco31I	GGTCTC	1 cut(s)	968
Eco47I	GGWCC	6 cut(s)	323, 1078, 1192, 2098, 2222, 2377
Eco53kI	GAGCTC	1 cut(s)	2628
Eco57I	CTGAAG	2 cut(s)	410, 2630
Eco81I	CCTNAGG	1 cut(s)	723
Eco91I	GGTNACC	2 cut(s)	935, 2108
EcoICRI	GAGCTC	1 cut(s)	2628
EcoO109I	RGGNCCY	7 cut(s)	718, 719, 1078, 1192, 1870, 1871, 2098
EcoO65I	GGTNACC	2 cut(s)	935, 2108
EcoRI	GAATTC	3 cut(s)	196, 247, 1744
EcoRII	CCWGG	5 cut(s)	709, 1360, 1670, 1956, 2467
EcoT14I	CCWWGG	3 cut(s)	1586, 1754, 2354
EcoT38I	GRGCYC	3 cut(s)	722, 1874, 2630
ErhI	CCWWGG	3 cut(s)	1586, 1754, 2354
FaqI	GGGAC	4 cut(s)	1091, 1205, 1415, 2372
FauI	CCCGC	1 cut(s)	2728
FauNDI	CATATG	1 cut(s)	1979
FbaI	TGATCA	1 cut(s)	1018
FblI	GTMKAC	1 cut(s)	1178
Fnu4HI	GCNGC	8 cut(s)	180, 374, 477, 564, 1518, 1691, 1803, 2247
FokI	GGATG	3 cut(s)	519, 1687, 2349
FriOI	GRGCYC	3 cut(s)	722, 1874, 2630
Fsp4HI	GCNGC	8 cut(s)	180, 374, 477, 564, 1518, 1691, 1803, 2247
FspBI	CTAG	8 cut(s)	684, 798, 1326, 1443, 1712, 1878, 1967, 2480
FspI	TGCGCA	1 cut(s)	869
GlaI	GCGC	1 cut(s)	869
GluI	GCNGC	8 cut(s)	180, 374, 477, 564, 1518, 1691, 1803, 2247
GsuI	CTGGAG	3 cut(s)	1167, 1275, 1383
HaeIII	GGCC	8 cut(s)	125, 314, 720, 740, 1360, 1771, 1872, 2516
HapII	CCGG	2 cut(s)	2334, 2622
HgaI	GACGC	1 cut(s)	2630
HhaI	GCGC	1 cut(s)	870
Hin1I	GRCGYC	1 cut(s)	2641
Hin6I	GCGC	1 cut(s)	868
HinP1I	GCGC	1 cut(s)	868
HincII	GTYRAC	3 cut(s)	1059, 2385, 2668
HindII	GTYRAC	3 cut(s)	1059, 2385, 2668
HindIII	AAGCTT	2 cut(s)	2051, 2506
HpaII	CCGG	2 cut(s)	2334, 2622
HphI	GGTGA	8 cut(s)	7, 1634, 1648, 1655, 2457, 2573, 2581, 2750
Hpy188III	TCNNGA	6 cut(s)	626, 1088, 1184, 1292, 2169, 2318
HpyAV	CCTTC	6 cut(s)	216, 295, 1558, 1816, 2228, 2668
HpyCH4IV	ACGT	1 cut(s)	592
HpySE526I	ACGT	1 cut(s)	592
Hsp92I	GRCGYC	1 cut(s)	2641
HspAI	GCGC	1 cut(s)	868
Ksp22I	TGATCA	1 cut(s)	1018
Kzo9I	GATC	8 cut(s)	406, 600, 628, 1018, 2416, 2427, 2693, 2779
Lsp1109I	GCAGC	7 cut(s)	166, 360, 463, 575, 1504, 1702, 1789
LweI	GCATC	5 cut(s)	997, 1056, 1469, 1621, 2285
MaeI	CTAG	8 cut(s)	684, 798, 1326, 1443, 1712, 1878, 1967, 2480
MaeII	ACGT	1 cut(s)	592
MalI	GATC	8 cut(s)	408, 602, 630, 1020, 2418, 2429, 2695, 2781
MboI	GATC	8 cut(s)	406, 600, 628, 1018, 2416, 2427, 2693, 2779
MflI	RGATCY	4 cut(s)	406, 600, 2416, 2427
MhlI	GDGCHC	5 cut(s)	722, 1595, 1608, 1874, 2630
MlsI	TGGCCA	2 cut(s)	314, 740
MluNI	TGGCCA	2 cut(s)	314, 740
MlyI	GAGTC	6 cut(s)	689, 971, 1054, 2166, 2354, 2741
MmeI	TCCRAC	2 cut(s)	1801, 2385
Mox20I	TGGCCA	2 cut(s)	314, 740
MroXI	GAANNNNTTC	2 cut(s)	956, 1033
MscI	TGGCCA	2 cut(s)	314, 740
MseI	TTAA	8 cut(s)	57, 663, 674, 1146, 1173, 1284, 1731, 2213
MslI	CAYNNNNRTG	1 cut(s)	1223
Msp20I	TGGCCA	2 cut(s)	314, 740
MspI	CCGG	2 cut(s)	2334, 2622
MspR9I	CCNGG	6 cut(s)	711, 1362, 1672, 1958, 2469, 2623
Mva1269I	GAATGC	2 cut(s)	337, 958
MvaI	CCWGG	5 cut(s)	711, 1362, 1672, 1958, 2469
NciI	CCSGG	1 cut(s)	2623
NdeI	CATATG	1 cut(s)	1979
NdeII	GATC	8 cut(s)	406, 600, 628, 1018, 2416, 2427, 2693, 2779
NmeAIII	GCCGAG	2 cut(s)	2492, 2617
NmuCI	GTSAC	3 cut(s)	1795, 1891, 2579
NsbI	TGCGCA	1 cut(s)	869
NspI	RCATGY	3 cut(s)	850, 874, 1486
PasI	CCCWGGG	1 cut(s)	1957
PciI	ACATGT	1 cut(s)	1482
PcsI	WCGNNNNNNNCGW	1 cut(s)	2181
PctI	GAATGC	2 cut(s)	337, 958
PdmI	GAANNNNTTC	2 cut(s)	956, 1033
PfeI	GAWTC	4 cut(s)	291, 809, 1049, 1703
PflMI	CCANNNNNTGG	1 cut(s)	1406
PfoI	TCCNGGA	1 cut(s)	2621
PkrI	GCNGC	8 cut(s)	181, 375, 478, 565, 1519, 1692, 1804, 2248
PleI	GAGTC	6 cut(s)	688, 971, 1054, 2166, 2354, 2741
PpsI	GAGTC	6 cut(s)	688, 971, 1054, 2166, 2354, 2741
PpuMI	RGGWCCY	3 cut(s)	1078, 1192, 2098
PscI	ACATGT	1 cut(s)	1482
Psp124BI	GAGCTC	1 cut(s)	2630
Psp1406I	AACGTT	1 cut(s)	592
Psp5II	RGGWCCY	3 cut(s)	1078, 1192, 2098
Psp6I	CCWGG	5 cut(s)	709, 1360, 1670, 1956, 2467
PspEI	GGTNACC	2 cut(s)	935, 2108
PspGI	CCWGG	5 cut(s)	709, 1360, 1670, 1956, 2467
PspOMI	GGGCCC	2 cut(s)	718, 1870
PspPPI	RGGWCCY	3 cut(s)	1078, 1192, 2098
PstI	CTGCAG	1 cut(s)	481
PstNI	CAGNNNCTG	1 cut(s)	485
PsuI	RGATCY	4 cut(s)	406, 600, 2416, 2427
RsaI	GTAC	9 cut(s)	657, 835, 875, 1496, 1971, 2461, 2600, 2685, 2786
RsaNI	GTAC	9 cut(s)	656, 834, 874, 1495, 1970, 2460, 2599, 2684, 2785
RseI	CAYNNNNRTG	1 cut(s)	1223
SacI	GAGCTC	1 cut(s)	2630
SaqAI	TTAA	8 cut(s)	57, 663, 674, 1146, 1173, 1284, 1731, 2213
SatI	GCNGC	8 cut(s)	180, 374, 477, 564, 1518, 1691, 1803, 2247
Sau3AI	GATC	8 cut(s)	406, 600, 628, 1018, 2416, 2427, 2693, 2779
ScaI	AGTACT	3 cut(s)	835, 1971, 2786
SchI	GAGTC	6 cut(s)	689, 971, 1054, 2166, 2354, 2741
ScrFI	CCNGG	6 cut(s)	711, 1362, 1672, 1958, 2469, 2623
SduI	GDGCHC	5 cut(s)	722, 1595, 1608, 1874, 2630
SfaNI	GCATC	5 cut(s)	997, 1056, 1469, 1621, 2285
SfcI	CTRYAG	3 cut(s)	256, 477, 2420
SinI	GGWCC	6 cut(s)	323, 1078, 1192, 2098, 2222, 2377
SmiMI	CAYNNNNRTG	1 cut(s)	1223
SmlI	CTYRAG	2 cut(s)	2008, 2287
SmoI	CTYRAG	2 cut(s)	2008, 2287
SsiI	CCGC	4 cut(s)	2247, 2535, 2596, 2735
SspMI	CTAG	8 cut(s)	684, 798, 1326, 1443, 1712, 1878, 1967, 2480
SstI	GAGCTC	1 cut(s)	2630
StyD4I	CCNGG	6 cut(s)	709, 1360, 1670, 1956, 2467, 2621
StyI	CCWWGG	3 cut(s)	1586, 1754, 2354
TaiI	ACGT	1 cut(s)	595
TaqI	TCGA	2 cut(s)	911, 1701
TaqII	GACCGA	1 cut(s)	950
TatI	WGTACW	7 cut(s)	655, 833, 873, 1969, 2459, 2683, 2784
TauI	GCSGC	1 cut(s)	2249
TfiI	GAWTC	4 cut(s)	291, 809, 1049, 1703
Tru1I	TTAA	8 cut(s)	57, 663, 674, 1146, 1173, 1284, 1731, 2213
Tru9I	TTAA	8 cut(s)	57, 663, 674, 1146, 1173, 1284, 1731, 2213
TseFI	GTSAC	3 cut(s)	1795, 1891, 2579
TseI	GCWGC	7 cut(s)	179, 373, 476, 563, 1517, 1690, 1802
Tsp45I	GTSAC	3 cut(s)	1795, 1891, 2579
TspDTI	ATGAA	7 cut(s)	35, 283, 689, 1026, 1227, 1875, 2112
TspGWI	ACGGA	1 cut(s)	1439
Van91I	CCANNNNNTGG	1 cut(s)	1406
VneI	GTGCAC	1 cut(s)	1604
VpaK11BI	GGWCC	6 cut(s)	323, 1078, 1192, 2098, 2222, 2377
XapI	RAATTY	8 cut(s)	196, 247, 902, 1454, 1553, 1744, 1862, 2321
XceI	RCATGY	3 cut(s)	850, 874, 1486
XmiI	GTMKAC	1 cut(s)	1178
XmnI	GAANNNNTTC	2 cut(s)	956, 1033
XspI	CTAG	8 cut(s)	684, 798, 1326, 1443, 1712, 1878, 1967, 2480
ZrmI	AGTACT	3 cut(s)	835, 1971, 2786

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.