pycom02g11400

gamma carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012856
Physical Location & Seq
Reverse (-)
8067089 .. 8068570
1482 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g11400.1

Sequence Viewer

Length: 807 bp
ATGGGGACCCTGGGCAGAGCAGTCTACACCGTTGGATTCTGGATCCGCGAGACTGGCCAAGCCATTGACCGCCTCGGTTCCCGCCTCCAAGGCAACTACTACTTCCAAGAACAGCTGTCGCGGCATCGAACGTTGATGAACGTGTTCGACAAAGCTCCGGTGGTCGATAAGGAGGCGTTTGTGGCGCCCAGTGCATCGATCATAGGCCAAGTTCAGGTGGGCCGAGGTTCCTCCATTTGGTACGGTTGCGTTTTGAGAGGCGATGTTAACAGCATCAGCATTGGGTCTGGGACCAACATTCAAGACAATTCCCTTGTTCATGTGGCAAAATCTAACTTGAGTGGCAAGGTTTTGCCTACCATTATCGGAGACAATGTCACTGTAGGCCACAGTGCTGTCTTGCATGGATGTACTGTGGAGGATGAAGCATTTGTGGGCATGGCTGCAACCTTGCTCGATGGGGTTTATGTCGAGAAACACGCCATGGTTGCTGCCGGAGCTCTTGTAAGGCAGAACACAAGGATCCCCTGTGGAGAGGTATGGGGAGGGAATCCAGCAAAGTTCCTGAGGAAGCTCACAGAAGATGAGATGGCCTTCATCTCCCAGTCAGCCTTAAACTATTCCAACTTGGCTCAGGTTCATGCAGCTGAAAATGCAAAGCCCCTCGATGAGATAGAGTTTGAGAAGGCGCTGCGAAAGAAGTTTGGACGCCGTGATGAGGAATATGACTCTATGTTGGGTGTCACTCGTGAGACACCGGCAGAGATTACTCTTCCTGATAACGTATTGCCACCTAAGTCTGCTTAA

Protein Analysis

269

Amino Acids

29.11

Weight (kDa)

6.09

Isoelectric Point (pI)

31.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LbH_EIF2B PF25084 91 - 175 3.8e-06 EIF2B subunit epsilon LbH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012856)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19580 AT1G19580
fragaria_vesca FvH4_1g12950
malus_domestica MD02G1144100.v1.1 MD15G1257700.v1.1
prunus_persica Prupe.7G157500_v2.0.a1 Prupe.7G157500_v2.0.a1
pyrus_communis pycom02g11400 pycom15g22540
rosa_chinensis RchiOBHm_Chr2g0101001
rosa_laevigata RLG00000017035
rosa_multiflora Rmu_sc0001862.1_g000005 Rmu_sc0007698.1_g000022 Rmu_sc0027174.1_g000001
rosa_roxburghii Rroxscaffold_2G00141660
rosa_rugosa Rorug02G0095100
rosa_samantha Rh2CG148600
rosa_wichuraiana Rw2G011160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 184
AccI GTMKAC 1 cut(s) 24
AccII CGCG 2 cut(s) 48, 121
AciI CCGC 4 cut(s) 46, 70, 82, 121
AclI AACGTT 1 cut(s) 131
AclWI GGATC 4 cut(s) 37, 50, 517, 530
AcoI YGGCCR 1 cut(s) 55
AcyI GRCGYC 2 cut(s) 185, 709
AfaI GTAC 2 cut(s) 242, 412
AfiI CCNNNNNNNGG 3 cut(s) 214, 237, 718
AflIII ACRYGT 1 cut(s) 141
AgsI TTSAA 1 cut(s) 302
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 5 cut(s) 115, 155, 500, 574, 647
AluI AGCT 5 cut(s) 115, 155, 500, 574, 647
Alw21I GWGCWC 1 cut(s) 502
Alw26I GTCTC 3 cut(s) 44, 363, 746
AlwI GGATC 4 cut(s) 37, 50, 517, 530
AoxI GGCC 5 cut(s) 55, 205, 220, 385, 591
ApeKI GCWGC 4 cut(s) 443, 491, 644, 691
Asp700I GAANNNNTTC 1 cut(s) 143
AspLEI GCGC 2 cut(s) 187, 691
AspS9I GGNCC 3 cut(s) 6, 220, 291
AvaII GGWCC 2 cut(s) 6, 291
AxyI CCTNAGG 1 cut(s) 566
BalI TGGCCA 1 cut(s) 57
BamHI GGATCC 2 cut(s) 42, 522
BanI GGYRCC 1 cut(s) 184
BanII GRGCYC 1 cut(s) 502
BauI CACGAG 1 cut(s) 747
Bbv12I GWGCWC 1 cut(s) 502
BbvI GCAGC 4 cut(s) 430, 478, 656, 678
BccI CCATC 2 cut(s) 452, 583
BceAI ACGGC 1 cut(s) 696
BciT130I CCWGG 1 cut(s) 11
BcoDI GTCTC 3 cut(s) 44, 363, 746
BfmI CTRYAG 1 cut(s) 381
BfoI RGCGCY 2 cut(s) 188, 692
BglI GCCNNNNNGGC 1 cut(s) 90
BisI GCNGC 5 cut(s) 122, 444, 492, 645, 692
BlsI GCNGC 5 cut(s) 123, 445, 493, 646, 693
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 2 cut(s) 6, 291
BmgT120I GGNCC 3 cut(s) 6, 220, 291
BmiI GGNNCC 8 cut(s) 7, 8, 44, 79, 186, 229, 292, 524
BmrFI CCNGG 1 cut(s) 11
BmrI ACTGGG 2 cut(s) 183, 598
BmsI GCATC 3 cut(s) 133, 203, 282
BmuI ACTGGG 2 cut(s) 183, 598
Bpu10I CCTNAGC 1 cut(s) 633
BpuEI CTTGAG 1 cut(s) 358
Bsa29I ATCGAT 1 cut(s) 197
BsaHI GRCGYC 2 cut(s) 185, 709
BsaJI CCNNGG 6 cut(s) 9, 10, 73, 88, 223, 483
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 4 cut(s) 360, 390, 489, 519
Bsc4I CCNNNNNNNGG 3 cut(s) 214, 237, 718
Bse118I RCCGGY 1 cut(s) 757
Bse1I ACTGG 3 cut(s) 58, 189, 604
Bse21I CCTNAGG 1 cut(s) 566
BseBI CCWGG 1 cut(s) 11
BseCI ATCGAT 1 cut(s) 197
BseDI CCNNGG 6 cut(s) 9, 10, 73, 88, 223, 483
BseGI GGATG 2 cut(s) 413, 427
BseLI CCNNNNNNNGG 3 cut(s) 214, 237, 718
BseMII CTCAG 2 cut(s) 557, 647
BseNI ACTGG 3 cut(s) 58, 189, 604
BseXI GCAGC 4 cut(s) 430, 478, 656, 678
Bsh1236I CGCG 2 cut(s) 48, 121
BshFI GGCC 5 cut(s) 57, 207, 222, 387, 593
BshNI GGYRCC 1 cut(s) 184
BshVI ATCGAT 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 502
BsiSI CCGG 3 cut(s) 158, 495, 758
BslFI GGGAC 2 cut(s) 19, 304
BslI CCNNNNNNNGG 3 cut(s) 214, 237, 718
BsmAI GTCTC 3 cut(s) 44, 363, 746
BsmFI GGGAC 2 cut(s) 19, 304
BsnI GGCC 5 cut(s) 57, 207, 222, 387, 593
Bsp1286I GDGCHC 1 cut(s) 502
Bsp143I GATC 3 cut(s) 42, 198, 522
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 4 cut(s) 46, 70, 82, 121
BspANI GGCC 5 cut(s) 57, 207, 222, 387, 593
BspCNI CTCAG 2 cut(s) 558, 646
BspDI ATCGAT 1 cut(s) 197
BspFNI CGCG 2 cut(s) 48, 121
BspLI GGNNCC 8 cut(s) 7, 8, 44, 79, 186, 229, 292, 524
BspPI GGATC 4 cut(s) 37, 50, 517, 530
BspT107I GGYRCC 1 cut(s) 184
BsrFI RCCGGY 1 cut(s) 757
BsrI ACTGG 3 cut(s) 58, 189, 604
BssAI RCCGGY 1 cut(s) 757
BssECI CCNNGG 6 cut(s) 9, 10, 73, 88, 223, 483
BssMI GATC 3 cut(s) 42, 198, 522
BssNI GRCGYC 2 cut(s) 185, 709
BssSI CACGAG 1 cut(s) 747
BssT1I CCWWGG 2 cut(s) 88, 483
Bst2BI CACGAG 1 cut(s) 747
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 5 cut(s) 31, 245, 382, 392, 415
Bst6I CTCTTC 1 cut(s) 777
BstACI GRCGYC 2 cut(s) 185, 709
BstDEI CTNAG 3 cut(s) 566, 633, 795
BstDSI CCRYGG 1 cut(s) 483
BstF5I GGATG 2 cut(s) 413, 427
BstFNI CGCG 2 cut(s) 48, 121
BstH2I RGCGCY 2 cut(s) 188, 692
BstHHI GCGC 2 cut(s) 187, 691
BstKTI GATC 3 cut(s) 45, 201, 525
BstMAI GTCTC 3 cut(s) 44, 363, 746
BstMBI GATC 3 cut(s) 42, 198, 522
BstMWI GCNNNNNNNGC 8 cut(s) 54, 90, 121, 182, 191, 488, 497, 653
BstNI CCWGG 1 cut(s) 11
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 1 cut(s) 381
BstUI CGCG 2 cut(s) 48, 121
BstV1I GCAGC 4 cut(s) 430, 478, 656, 678
BstX2I RGATCY 2 cut(s) 42, 522
BstYI RGATCY 2 cut(s) 42, 522
Bsu15I ATCGAT 1 cut(s) 197
Bsu36I CCTNAGG 1 cut(s) 566
BsuRI GGCC 5 cut(s) 57, 207, 222, 387, 593
BsuTUI ATCGAT 1 cut(s) 197
BtgI CCRYGG 1 cut(s) 483
BtgZI GCGATG 1 cut(s) 276
BtsCI GGATG 2 cut(s) 413, 427
BtsIMutI CAGTG 3 cut(s) 196, 378, 397
CfoI GCGC 2 cut(s) 187, 691
Cfr10I RCCGGY 1 cut(s) 757
Cfr13I GGNCC 3 cut(s) 6, 220, 291
ClaI ATCGAT 1 cut(s) 197
CseI GACGC 1 cut(s) 717
Csp6I GTAC 2 cut(s) 241, 411
CviAII CATG 5 cut(s) 320, 404, 439, 484, 641
CviQI GTAC 2 cut(s) 241, 411
DdeI CTNAG 3 cut(s) 566, 633, 795
DinI GGCGCC 1 cut(s) 186
DpnI GATC 3 cut(s) 44, 200, 524
DpnII GATC 3 cut(s) 42, 198, 522
EaeI YGGCCR 1 cut(s) 55
Eam1104I CTCTTC 1 cut(s) 777
EarI CTCTTC 1 cut(s) 777
Ecl136II GAGCTC 1 cut(s) 500
Eco130I CCWWGG 2 cut(s) 88, 483
Eco24I GRGCYC 1 cut(s) 502
Eco47I GGWCC 2 cut(s) 6, 291
Eco53kI GAGCTC 1 cut(s) 500
Eco81I CCTNAGG 1 cut(s) 566
EcoICRI GAGCTC 1 cut(s) 500
EcoO109I RGGNCCY 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 9
EcoT14I CCWWGG 2 cut(s) 88, 483
EcoT38I GRGCYC 1 cut(s) 502
EgeI GGCGCC 1 cut(s) 186
EheI GGCGCC 1 cut(s) 186
ErhI CCWWGG 2 cut(s) 88, 483
FaeI CATG 5 cut(s) 323, 407, 442, 487, 644
FaqI GGGAC 2 cut(s) 19, 304
FatI CATG 5 cut(s) 319, 403, 438, 483, 640
FauI CCCGC 1 cut(s) 89
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 5 cut(s) 122, 444, 492, 645, 692
FokI GGATG 2 cut(s) 420, 434
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 5 cut(s) 122, 444, 492, 645, 692
GlaI GCGC 2 cut(s) 186, 690
GluI GCNGC 5 cut(s) 122, 444, 492, 645, 692
HaeII RGCGCY 2 cut(s) 188, 692
HaeIII GGCC 5 cut(s) 57, 207, 222, 387, 593
HapII CCGG 3 cut(s) 158, 495, 758
HgaI GACGC 1 cut(s) 717
HhaI GCGC 2 cut(s) 187, 691
Hin1I GRCGYC 2 cut(s) 185, 709
Hin1II CATG 5 cut(s) 323, 407, 442, 487, 644
Hin6I GCGC 2 cut(s) 185, 689
HinP1I GCGC 2 cut(s) 185, 689
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 3 cut(s) 36, 550, 728
HpaI GTTAAC 1 cut(s) 268
HpaII CCGG 3 cut(s) 158, 495, 758
Hpy166II GTNNAC 2 cut(s) 25, 268
Hpy188I TCNGA 1 cut(s) 368
Hpy188III TCNNGA 6 cut(s) 40, 302, 472, 565, 749, 776
Hpy8I GTNNAC 2 cut(s) 25, 268
HpyAV CCTTC 2 cut(s) 604, 679
HpyCH4III ACNGT 5 cut(s) 31, 245, 382, 392, 415
HpyCH4IV ACGT 3 cut(s) 131, 141, 783
HpyCH4V TGCA 5 cut(s) 194, 403, 446, 644, 656
HpyF10VI GCNNNNNNNGC 8 cut(s) 54, 90, 121, 182, 191, 488, 497, 653
HpyF3I CTNAG 3 cut(s) 566, 633, 795
HpySE526I ACGT 3 cut(s) 131, 141, 783
Hsp92I GRCGYC 2 cut(s) 185, 709
Hsp92II CATG 5 cut(s) 323, 407, 442, 487, 644
HspAI GCGC 2 cut(s) 185, 689
KasI GGCGCC 1 cut(s) 184
KflI GGGWCCC 1 cut(s) 6
KspAI GTTAAC 1 cut(s) 268
Kzo9I GATC 3 cut(s) 42, 198, 522
LmnI GCTCC 2 cut(s) 160, 497
Lsp1109I GCAGC 4 cut(s) 430, 478, 656, 678
LweI GCATC 3 cut(s) 133, 203, 282
MaeII ACGT 3 cut(s) 131, 141, 783
MaeIII GTNAC 2 cut(s) 376, 742
MalI GATC 3 cut(s) 44, 200, 524
MboI GATC 3 cut(s) 42, 198, 522
MboII GAAGA 2 cut(s) 593, 764
MflI RGATCY 2 cut(s) 42, 522
MhlI GDGCHC 1 cut(s) 502
MlsI TGGCCA 1 cut(s) 57
MluCI AATT 1 cut(s) 307
MluNI TGGCCA 1 cut(s) 57
Mly113I GGCGCC 1 cut(s) 185
MlyI GAGTC 1 cut(s) 722
MmeI TCCRAC 2 cut(s) 13, 648
Mox20I TGGCCA 1 cut(s) 57
MroXI GAANNNNTTC 1 cut(s) 143
MscI TGGCCA 1 cut(s) 57
MseI TTAA 3 cut(s) 267, 614, 805
Msp20I TGGCCA 1 cut(s) 57
MspA1I CMGCKG 2 cut(s) 115, 647
MspI CCGG 3 cut(s) 158, 495, 758
MspR9I CCNGG 1 cut(s) 11
MvaI CCWGG 1 cut(s) 11
MvnI CGCG 2 cut(s) 48, 121
MwoI GCNNNNNNNGC 8 cut(s) 54, 90, 121, 182, 191, 488, 497, 653
NarI GGCGCC 1 cut(s) 185
NcoI CCATGG 1 cut(s) 483
NdeII GATC 3 cut(s) 42, 198, 522
NlaIII CATG 5 cut(s) 323, 407, 442, 487, 644
NlaIV GGNNCC 8 cut(s) 7, 8, 44, 79, 186, 229, 292, 524
NmeAIII GCCGAG 1 cut(s) 248
NmuCI GTSAC 2 cut(s) 376, 742
PasI CCCWGGG 1 cut(s) 10
PdmI GAANNNNTTC 1 cut(s) 143
PfeI GAWTC 2 cut(s) 36, 550
PflFI GACNNNGTC 1 cut(s) 374
PkrI GCNGC 5 cut(s) 123, 445, 493, 646, 693
PleI GAGTC 1 cut(s) 722
PluTI GGCGCC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 722
PpuMI RGGWCCY 1 cut(s) 6
Psp124BI GAGCTC 1 cut(s) 502
Psp1406I AACGTT 1 cut(s) 131
Psp5II RGGWCCY 1 cut(s) 6
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 8 cut(s) 7, 8, 44, 79, 186, 229, 292, 524
PspPI GGNCC 3 cut(s) 6, 220, 291
PspPPI RGGWCCY 1 cut(s) 6
PsuI RGATCY 2 cut(s) 42, 522
PsyI GACNNNGTC 1 cut(s) 374
PvuII CAGCTG 2 cut(s) 115, 647
RsaI GTAC 2 cut(s) 242, 412
RsaNI GTAC 2 cut(s) 241, 411
SacI GAGCTC 1 cut(s) 502
SaqAI TTAA 3 cut(s) 267, 614, 805
SatI GCNGC 5 cut(s) 122, 444, 492, 645, 692
Sau3AI GATC 3 cut(s) 42, 198, 522
Sau96I GGNCC 3 cut(s) 6, 220, 291
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 502
SfaNI GCATC 3 cut(s) 133, 203, 282
SfcI CTRYAG 1 cut(s) 381
SfoI GGCGCC 1 cut(s) 186
SinI GGWCC 2 cut(s) 6, 291
SmlI CTYRAG 1 cut(s) 337
SmoI CTYRAG 1 cut(s) 337
Sse9I AATT 1 cut(s) 307
SsiI CCGC 4 cut(s) 46, 70, 82, 121
SspDI GGCGCC 1 cut(s) 184
SstI GAGCTC 1 cut(s) 502
StyD4I CCNGG 1 cut(s) 9
StyI CCWWGG 2 cut(s) 88, 483
TaaI ACNGT 5 cut(s) 31, 245, 382, 392, 415
TaiI ACGT 3 cut(s) 134, 144, 786
TaqI TCGA 7 cut(s) 127, 147, 165, 197, 456, 471, 666
TasI AATT 1 cut(s) 307
TatI WGTACW 1 cut(s) 410
TauI GCSGC 1 cut(s) 124
TfiI GAWTC 2 cut(s) 36, 550
Tru1I TTAA 3 cut(s) 267, 614, 805
Tru9I TTAA 3 cut(s) 267, 614, 805
TscAI CASTG 3 cut(s) 196, 385, 397
TseFI GTSAC 2 cut(s) 376, 742
TseI GCWGC 4 cut(s) 443, 491, 644, 691
Tsp45I GTSAC 2 cut(s) 376, 742
TspDTI ATGAA 5 cut(s) 152, 308, 438, 586, 629
TspRI CASTG 3 cut(s) 196, 385, 397
Tth111I GACNNNGTC 1 cut(s) 374
VpaK11BI GGWCC 2 cut(s) 6, 291
XmiI GTMKAC 1 cut(s) 24
XmnI GAANNNNTTC 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.