Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0008879

Physical Location & Seq

Genomic Coordinates Forward (+)

22841909 .. 22845016

Gene Structure 3108 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g24740.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2457 bp

ATGGAGTTAAAAGCTTTGGTGATTGAGGTAAGTTCTTTGGCTCAAAACTCAGAAATTCAAAGAGAAGTAGTGACTGAATTTGTAATCTTGGTTGGGAAATTAGCTCCAATTTTTGATGGATTAATGGATACTGAGTTTCTTAATCACCAACCATTGAGAAAAGCTGTTGAGTCTCTTGGGTTGGAACTTAAGAGAGCCAAGGCTTTGCTGAAAACCCAAGAGACAAATTCATTTGTTGGACAAGTAGAGGATGTGGCTCATGATCTTGGGAGGTCATTAGGGTTAGTGCTCTTGGCAAGTTTAGAAGTGTCCACAGATTTGAAAGACAAGGTTGGGGGCTTACACAAGGATTTGATGAGTACGCGGTTTGATGTGAGTTCGTTTGCAAGTACTAGTTATGGTTCTGGTGTTGTTAGTGAGTTGGAAGTGGAAGTGGAAGAGGAAATACAAGAGGAGAAAACGGTTTGTTTTGGTATTGATGATGTTTCTTTGCAAATTAAGTATGGTGATGATGAACAACTTAAATTTGCTCTTTTGGAATTAAATGAGTTGATTGTAGACAAAAGGGTTGGATATGAATGGATTAATGATGAAGGTGTTATTCCGATTCTGTTTAATCGGTTGAGTTCAAGTAATTCAGAGAATCGGCTTTGTATACTTCGATTGTTGAGAAGCCTTGCTTCAGATAATGCTGATAACAAGGAGAAAATGGCAGATGTTGGTTTTTTGTCCGCGGCCGTGAAATCATTGGTACGGGATGAAGAAGAAAGGAAAGAAGCTGTGGGGTTTTTGCTGTATCTCTCAGATCTTCAGTCAGTAAGGCGCCGGCTTGGGCGCATTCAAGGGTGTATCGTTATGTTGCTTGCCTTGCTAAATGAGGATGACCTTGTCGCTTCACATAATGCAGGGAAGTTGTTGAATGCATTATCTAGCAACACGCAGAATGCACTTCATATGGCAGAGGCTGGTTACTTTAAGCCCCTAGTGCAGTACCTCGAAGAAGGTTCGGACATGAGTAAAATCCTGATGGCAACAGCACTCTCAAGGATGGAGCTCACTGATCAAAGTCGAGCTTCCCTCGGGGAAAATGGTGCAATTGAACCCCTTGTCAGAATGTTTAGCATAGGGAAGCTCGAAGCCAAGTTATCCGCTTTAAGTGCCTTGCAAAATCTATCAAACCTGACAGAAAATGTCCAACGGTTGATCAGTTCAGGCATCGTAGCATCTCTGCTTCAGCTCCTCTTCTCTGTAACATCAGTACTTATGGCTCTTCGGGAGCCTGTATCAGCGATTCTTGCAAGGATTGCTCAATCAGAATCTATTCTTGTGAATTCAGATGTTGCTCAGCAGATGCTCTCCCTTTTAAATCTCACCAGTCCGGTAATTCAAAATCACCTATTGCAAGCACTTAATAGTATTGCCTCCCATTCCAGAGCAGGCAAAGTTAGAAGAAGGATGAAAGAAAATGGCGCCTTTCAGCTTCTCCTACCCTTCCTCATGGAAACTAACATTCAAATCAGGAGCAGTGCCTTGAGTTTGCTTTACACTCTTTCTAAATATTTATCGGAAGAGTTGACAGACCAACTTGAAGAAACCAATATCAGAAAAATCATCATTATCATCTCAACATCAACATCTAACTGCGAAAAAGCCGCTGGAGTTGGCATACTTAGTCATCTTCCCATTAGTGACAAAAAGGTGACGGATATATTAAAGAGAGCAAATTTGGTACCGATAATGGTATCCATAATGAATGCAAGATCTGAAATTCCAGCGGAGAAGATGTGCTGTTTAGCAGAAAGCATTACAGGTCTATTAATTCGGTTCACAAATCCTGCTGATAAGAAACTACAACTTTATTCAGCAGAACAAGGGGTAATTCCTTTACTTGTCAAGTTACTCTCGAGTGGATCACCTGTTACTAAATGCAGGGCTGCAACGTCACTAGCTCAGTTTTCGCAGAATTCATCATCTCTAAAGAAGTCGATAAAACCAAGGTGGACATGCGTTCCTCCTTCTCAAGATGCTGTTTGTGAAGTTCATGATGGAAAATGCTTTGTGAAAAGCACATTTTGTCTGGTCAAGGCAGGTGCTATCTCTCCTGTAATCCAAATATTGCAAGGTAAGGAGAGGGAAGCTGATGAAGCAGCACTGAGCGTCCTTGCAACCCTCTTGCACGACGACATTTGGGAAAACGGAAGCAAGTACATAGCCAACAAGTCAGGAATTCCGGCCATCATCAAAGTCCTCGAATCCGGGAGCATCAAGGCTCAAGAGAAAGCACTACGGATATTGGAGAAGATATTTGGAGTTGAGGAACACCGAGTAACATACGGGGAATCTGCTCAGGCAGTGCTGATTGATCTAGCCCAGCAGGGTGACTCCAGATTGAAATCAACCACTGCAAAATTACTGGCGCAACTAGAGCTATTGCAAGCTCAATCTAGTTACTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm KAP

Protein Analysis

819

Amino Acids

89.64

Weight (kDa)

5.66

Isoelectric Point (pI)

41.47

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008879)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g02490 FvH4_7g02490 FvH4_7g02490
malus_domestica	MD02G1293000.v1.1 MD07G1034100.v1.1
prunus_persica	Prupe.2G030600_v2.0.a1 Prupe.2G030600_v2.0.a1
pyrus_communis	pycom02g24740 pycom07g02410
rosa_chinensis	RchiOBHm_Chr1g0321131
rosa_laevigata	RLG00000030449
rosa_multiflora	Rmu_sc0004660.1_g000007 Rmu_sc0007977.1_g000007
rosa_roxburghii	Rroxscaffold_160G00433740 Rroxscaffold_4G00328070
rosa_rugosa	Rorug01G0031200 Rorug01G0031300
rosa_samantha	Rh1AG043200 Rh1BG039900 Rh1CG045300 Rh1DG049900
rosa_wichuraiana	Rw0G001070 Rw1G003790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2078
Acc36I	ACCTGC	1 cut(s)	2078
Acc65I	GGTACC	1 cut(s)	1729
AccB1I	GGYRCC	3 cut(s)	822, 1469, 1729
AccI	GTMKAC	2 cut(s)	558, 655
AccII	CGCG	2 cut(s)	364, 734
AciI	CCGC	6 cut(s)	364, 732, 734, 1149, 1653, 1775
AclWI	GGATC	1 cut(s)	1918
AcoI	YGGCCR	2 cut(s)	735, 2232
AcsI	RAATTY	9 cut(s)	54, 77, 226, 524, 1330, 1723, 1767, 1963, 2226
AcuI	CTGAAG	3 cut(s)	666, 794, 1217
AcyI	GRCGYC	2 cut(s)	823, 1470
AfaI	GTAC	7 cut(s)	361, 391, 753, 992, 1260, 1731, 2207
AflII	CTTAAG	1 cut(s)	188
AhlI	ACTAGT	1 cut(s)	392
Alw21I	GWGCWC	2 cut(s)	291, 1056
Alw26I	GTCTC	2 cut(s)	177, 215
AlwI	GGATC	1 cut(s)	1918
AlwNI	CAGNNNCTG	1 cut(s)	965
Ama87I	CYCGRG	2 cut(s)	1079, 1903
AoxI	GGCC	2 cut(s)	735, 2232
ApeKI	GCWGC	2 cut(s)	1934, 2147
ApoI	RAATTY	9 cut(s)	54, 77, 226, 524, 1330, 1723, 1767, 1963, 2226
AseI	ATTAAT	3 cut(s)	122, 585, 1817
Asp700I	GAANNNNTTC	1 cut(s)	1320
Asp718I	GGTACC	1 cut(s)	1729
AspLEI	GCGC	4 cut(s)	825, 837, 1472, 2419
AsuC2I	CCSGG	1 cut(s)	2257
AsuHPI	GGTGA	8 cut(s)	31, 137, 518, 1363, 1385, 1711, 1905, 2390
AvaI	CYCGRG	2 cut(s)	1079, 1903
BanI	GGYRCC	3 cut(s)	822, 1469, 1729
BanII	GRGCYC	1 cut(s)	1056
BarI	GAAGNNNNNNTAC	2 cut(s)	429, 461
Bbv12I	GWGCWC	2 cut(s)	291, 1056
BbvI	GCAGC	2 cut(s)	1921, 2159
BccI	CCATC	5 cut(s)	110, 1021, 1042, 2039, 2243
BceAI	ACGGC	1 cut(s)	722
BciVI	GTATCC	2 cut(s)	121, 1753
BclI	TGATCA	2 cut(s)	1060, 1203
BcnI	CCSGG	1 cut(s)	2257
BcoDI	GTCTC	2 cut(s)	177, 215
BcuI	ACTAGT	1 cut(s)	392
BfaI	CTAG	7 cut(s)	393, 930, 983, 1946, 2366, 2423, 2445
BfoI	RGCGCY	2 cut(s)	826, 1473
BfrI	CTTAAG	1 cut(s)	188
BfuAI	ACCTGC	1 cut(s)	2078
BfuI	GTATCC	2 cut(s)	121, 1753
BglII	AGATCT	2 cut(s)	805, 1760
BisI	GCNGC	4 cut(s)	735, 1653, 1935, 2148
BlpI	GCTNAGC	1 cut(s)	1344
BlsI	GCNGC	4 cut(s)	736, 1654, 1936, 2149
BmcAI	AGTACT	2 cut(s)	391, 1260
Bme1390I	CCNGG	1 cut(s)	2257
BmeT110I	CYCGRG	2 cut(s)	1079, 1903
BmiI	GGNNCC	4 cut(s)	824, 1278, 1471, 1731
BmrFI	CCNGG	1 cut(s)	2257
BmsI	GCATC	5 cut(s)	1224, 1232, 1341, 2014, 2271
BplI	GAGNNNNNCTC	2 cut(s)	1062, 1094
BpmI	CTGGAG	2 cut(s)	1677, 2368
Bpu10I	CCTNAGC	1 cut(s)	2346
Bpu1102I	GCTNAGC	1 cut(s)	1344
BpuEI	CTTGAG	4 cut(s)	1027, 1552, 2004, 2256
BpuMI	CCSGG	1 cut(s)	2257
BsaBI	GATNNNNATC	1 cut(s)	2392
BsaHI	GRCGYC	2 cut(s)	823, 1470
BsaJI	CCNNGG	4 cut(s)	198, 732, 1078, 1994
BsaWI	WCCGGW	1 cut(s)	1378
BsaXI	ACNNNNNCTCC	2 cut(s)	1769, 1799
Bse118I	RCCGGY	1 cut(s)	825
Bse1I	ACTGG	2 cut(s)	1374, 2418
Bse8I	GATNNNNATC	1 cut(s)	2392
BseDI	CCNNGG	4 cut(s)	198, 732, 1078, 1994
BseGI	GGATG	5 cut(s)	256, 763, 886, 1053, 1461
BseJI	GATNNNNATC	1 cut(s)	2392
BseMII	CTCAG	7 cut(s)	63, 123, 816, 1358, 1964, 2144, 2360
BseNI	ACTGG	2 cut(s)	1374, 2418
BseRI	GAGGAG	2 cut(s)	467, 1229
BseX3I	CGGCCG	1 cut(s)	735
BseXI	GCAGC	2 cut(s)	1921, 2159
BseYI	CCCAGC	1 cut(s)	2370
BsgI	GTGCAG	1 cut(s)	1007
Bsh1236I	CGCG	2 cut(s)	364, 734
Bsh1285I	CGRYCG	1 cut(s)	738
BshFI	GGCC	2 cut(s)	737, 2234
BshNI	GGYRCC	3 cut(s)	822, 1469, 1729
BsiEI	CGRYCG	1 cut(s)	738
BsiHKAI	GWGCWC	2 cut(s)	291, 1056
BsiHKCI	CYCGRG	2 cut(s)	1079, 1903
BsiSI	CCGG	4 cut(s)	826, 1379, 2231, 2256
BsmAI	GTCTC	2 cut(s)	177, 215
BsmI	GAATGC	4 cut(s)	837, 925, 949, 1759
BsnI	GGCC	2 cut(s)	737, 2234
BsoBI	CYCGRG	2 cut(s)	1079, 1903
Bsp1286I	GDGCHC	2 cut(s)	291, 1056
Bsp143I	GATC	7 cut(s)	262, 805, 1060, 1203, 1760, 1910, 2362
Bsp1720I	GCTNAGC	1 cut(s)	1344
BspACI	CCGC	6 cut(s)	364, 732, 734, 1149, 1653, 1775
BspANI	GGCC	2 cut(s)	737, 2234
BspCNI	CTCAG	7 cut(s)	62, 124, 815, 1357, 1963, 2145, 2359
BspFNI	CGCG	2 cut(s)	364, 734
BspHI	TCATGA	2 cut(s)	259, 2041
BspLI	GGNNCC	4 cut(s)	824, 1278, 1471, 1731
BspMI	ACCTGC	1 cut(s)	2078
BspPI	GGATC	1 cut(s)	1918
BspQI	GCTCTTC	1 cut(s)	1275
BspT107I	GGYRCC	3 cut(s)	822, 1469, 1729
BspTI	CTTAAG	1 cut(s)	188
BsrFI	RCCGGY	1 cut(s)	825
BsrI	ACTGG	2 cut(s)	1374, 2418
BssAI	RCCGGY	1 cut(s)	825
BssECI	CCNNGG	4 cut(s)	198, 732, 1078, 1994
BssMI	GATC	7 cut(s)	262, 805, 1060, 1203, 1760, 1910, 2362
BssNAI	GTATAC	1 cut(s)	656
BssNI	GRCGYC	2 cut(s)	823, 1470
BssT1I	CCWWGG	2 cut(s)	198, 1994
Bst1107I	GTATAC	1 cut(s)	656
Bst4CI	ACNGT	2 cut(s)	463, 1200
Bst6I	CTCTTC	4 cut(s)	432, 1247, 1275, 1563
BstACI	GRCGYC	2 cut(s)	823, 1470
BstAFI	CTTAAG	1 cut(s)	188
BstAPI	GCANNNNNTGC	1 cut(s)	1304
BstC8I	GCNNGC	5 cut(s)	827, 864, 1404, 1438, 2436
BstDEI	CTNAG	8 cut(s)	49, 132, 802, 1344, 1670, 1950, 2153, 2346
BstDSI	CCRYGG	1 cut(s)	732
BstF5I	GGATG	5 cut(s)	256, 763, 886, 1053, 1461
BstFNI	CGCG	2 cut(s)	364, 734
BstH2I	RGCGCY	2 cut(s)	826, 1473
BstHHI	GCGC	4 cut(s)	825, 837, 1472, 2419
BstKTI	GATC	7 cut(s)	265, 808, 1063, 1206, 1763, 1913, 2365
BstMAI	GTCTC	2 cut(s)	177, 215
BstMBI	GATC	7 cut(s)	262, 805, 1060, 1203, 1760, 1910, 2362
BstMCI	CGRYCG	1 cut(s)	738
BstMWI	GCNNNNNNNGC	7 cut(s)	868, 985, 1157, 1295, 1304, 2144, 2425
BstNSI	RCATGY	1 cut(s)	2007
BstSCI	CCNGG	1 cut(s)	2255
BstUI	CGCG	2 cut(s)	364, 734
BstV1I	GCAGC	2 cut(s)	1921, 2159
BstX2I	RGATCY	2 cut(s)	805, 1760
BstYI	RGATCY	2 cut(s)	805, 1760
BstZ17I	GTATAC	1 cut(s)	656
BstZI	CGGCCG	1 cut(s)	735
BsuI	GTATCC	2 cut(s)	121, 1753
BsuRI	GGCC	2 cut(s)	737, 2234
BtgI	CCRYGG	1 cut(s)	732
BtsCI	GGATG	5 cut(s)	256, 763, 886, 1053, 1461
BtsI	GCAGTG	3 cut(s)	1531, 2358, 2400
BtsIMutI	CAGTG	5 cut(s)	1056, 1531, 2150, 2358, 2400
BveI	ACCTGC	1 cut(s)	2078
Cac8I	GCNNGC	5 cut(s)	827, 864, 1404, 1438, 2436
CaiI	CAGNNNCTG	1 cut(s)	965
CciI	TCATGA	2 cut(s)	259, 2041
CfoI	GCGC	4 cut(s)	825, 837, 1472, 2419
Cfr10I	RCCGGY	1 cut(s)	825
Cfr42I	CCGCGG	1 cut(s)	735
CseI	GACGC	1 cut(s)	2146
Csp6I	GTAC	7 cut(s)	360, 390, 752, 991, 1259, 1730, 2206
CviAII	CATG	5 cut(s)	260, 1012, 1498, 2004, 2042
CviQI	GTAC	7 cut(s)	360, 390, 752, 991, 1259, 1730, 2206
DdeI	CTNAG	8 cut(s)	49, 132, 802, 1344, 1670, 1950, 2153, 2346
DinI	GGCGCC	2 cut(s)	824, 1471
DpnI	GATC	7 cut(s)	264, 807, 1062, 1205, 1762, 1912, 2364
DpnII	GATC	7 cut(s)	262, 805, 1060, 1203, 1760, 1910, 2362
DraI	TTTAAA	1 cut(s)	1365
EaeI	YGGCCR	2 cut(s)	735, 2232
EagI	CGGCCG	1 cut(s)	735
Eam1104I	CTCTTC	4 cut(s)	432, 1247, 1275, 1563
EarI	CTCTTC	4 cut(s)	432, 1247, 1275, 1563
Ecl136II	GAGCTC	1 cut(s)	1054
EclXI	CGGCCG	1 cut(s)	735
Eco130I	CCWWGG	2 cut(s)	198, 1994
Eco24I	GRGCYC	1 cut(s)	1056
Eco52I	CGGCCG	1 cut(s)	735
Eco53kI	GAGCTC	1 cut(s)	1054
Eco57I	CTGAAG	3 cut(s)	666, 794, 1217
Eco88I	CYCGRG	2 cut(s)	1079, 1903
EcoICRI	GAGCTC	1 cut(s)	1054
EcoRI	GAATTC	3 cut(s)	1330, 1963, 2226
EcoT14I	CCWWGG	2 cut(s)	198, 1994
EcoT22I	ATGCAT	1 cut(s)	925
EcoT38I	GRGCYC	1 cut(s)	1056
EgeI	GGCGCC	2 cut(s)	824, 1471
EheI	GGCGCC	2 cut(s)	824, 1471
ErhI	CCWWGG	2 cut(s)	198, 1994
FaeI	CATG	5 cut(s)	263, 1015, 1501, 2007, 2045
FalI	AAGNNNNNCTT	4 cut(s)	664, 696, 1057, 1089
FatI	CATG	5 cut(s)	259, 1011, 1497, 2003, 2041
FauNDI	CATATG	1 cut(s)	954
FbaI	TGATCA	2 cut(s)	1060, 1203
FblI	GTMKAC	2 cut(s)	558, 655
Fnu4HI	GCNGC	4 cut(s)	735, 1653, 1935, 2148
FokI	GGATG	5 cut(s)	263, 770, 893, 1060, 1468
FriOI	GRGCYC	1 cut(s)	1056
Fsp4HI	GCNGC	4 cut(s)	735, 1653, 1935, 2148
FspBI	CTAG	7 cut(s)	393, 930, 983, 1946, 2366, 2423, 2445
GlaI	GCGC	4 cut(s)	824, 836, 1471, 2418
GluI	GCNGC	4 cut(s)	735, 1653, 1935, 2148
GsaI	CCCAGC	1 cut(s)	2374
GsuI	CTGGAG	2 cut(s)	1677, 2368
HaeII	RGCGCY	2 cut(s)	826, 1473
HaeIII	GGCC	2 cut(s)	737, 2234
HapII	CCGG	4 cut(s)	826, 1379, 2231, 2256
HgaI	GACGC	1 cut(s)	2146
HhaI	GCGC	4 cut(s)	825, 837, 1472, 2419
Hin1I	GRCGYC	2 cut(s)	823, 1470
Hin1II	CATG	5 cut(s)	263, 1015, 1501, 2007, 2045
Hin6I	GCGC	4 cut(s)	823, 835, 1470, 2417
HinP1I	GCGC	4 cut(s)	823, 835, 1470, 2417
HincII	GTYRAC	1 cut(s)	1575
HindII	GTYRAC	1 cut(s)	1575
HindIII	AAGCTT	1 cut(s)	12
HinfI	GANTC	8 cut(s)	170, 607, 643, 1291, 1316, 2252, 2339, 2381
HpaII	CCGG	4 cut(s)	826, 1379, 2231, 2256
HphI	GGTGA	8 cut(s)	31, 137, 518, 1363, 1385, 1711, 1905, 2390
Hpy166II	GTNNAC	6 cut(s)	312, 559, 656, 1575, 1827, 2001
Hpy8I	GTNNAC	6 cut(s)	312, 559, 656, 1575, 1827, 2001
Hpy99I	CGWCG	1 cut(s)	2183
HpyAV	CCTTC	5 cut(s)	587, 995, 1446, 1501, 2025
HpyCH4III	ACNGT	2 cut(s)	463, 1200
HpyCH4IV	ACGT	1 cut(s)	1940
HpyF10VI	GCNNNNNNNGC	7 cut(s)	868, 985, 1157, 1295, 1304, 2144, 2425
HpyF3I	CTNAG	8 cut(s)	49, 132, 802, 1344, 1670, 1950, 2153, 2346
HpySE526I	ACGT	1 cut(s)	1940
Hsp92I	GRCGYC	2 cut(s)	823, 1470
Hsp92II	CATG	5 cut(s)	263, 1015, 1501, 2007, 2045
HspAI	GCGC	4 cut(s)	823, 835, 1470, 2417
KasI	GGCGCC	2 cut(s)	822, 1469
KpnI	GGTACC	1 cut(s)	1733
KroI	GCCGGC	1 cut(s)	825
KroNI	GCCGGC	1 cut(s)	827
Ksp22I	TGATCA	2 cut(s)	1060, 1203
KspI	CCGCGG	1 cut(s)	735
Kzo9I	GATC	7 cut(s)	262, 805, 1060, 1203, 1760, 1910, 2362
LguI	GCTCTTC	1 cut(s)	1275
LmnI	GCTCC	6 cut(s)	109, 1051, 1242, 1276, 1521, 2259
Lsp1109I	GCAGC	2 cut(s)	1921, 2159
LweI	GCATC	5 cut(s)	1224, 1232, 1341, 2014, 2271
MaeI	CTAG	7 cut(s)	393, 930, 983, 1946, 2366, 2423, 2445
MaeII	ACGT	1 cut(s)	1940
MalI	GATC	7 cut(s)	264, 807, 1062, 1205, 1762, 1912, 2364
MboI	GATC	7 cut(s)	262, 805, 1060, 1203, 1760, 1910, 2362
MfeI	CAATTG	1 cut(s)	1095
MflI	RGATCY	2 cut(s)	805, 1760
MhlI	GDGCHC	2 cut(s)	291, 1056
Mly113I	GGCGCC	2 cut(s)	823, 1470
MlyI	GAGTC	2 cut(s)	179, 2375
MmeI	TCCRAC	5 cut(s)	162, 217, 402, 550, 1219
Mph1103I	ATGCAT	1 cut(s)	925
MroNI	GCCGGC	1 cut(s)	825
MroXI	GAANNNNTTC	1 cut(s)	1320
MspA1I	CMGCKG	3 cut(s)	734, 1655, 1775
MspCI	CTTAAG	1 cut(s)	188
MspI	CCGG	4 cut(s)	826, 1379, 2231, 2256
MspR9I	CCNGG	1 cut(s)	2257
MunI	CAATTG	1 cut(s)	1095
Mva1269I	GAATGC	4 cut(s)	837, 925, 949, 1759
MvnI	CGCG	2 cut(s)	364, 734
MwoI	GCNNNNNNNGC	7 cut(s)	868, 985, 1157, 1295, 1304, 2144, 2425
NaeI	GCCGGC	1 cut(s)	827
NarI	GGCGCC	2 cut(s)	823, 1470
NciI	CCSGG	1 cut(s)	2257
NdeI	CATATG	1 cut(s)	954
NdeII	GATC	7 cut(s)	262, 805, 1060, 1203, 1760, 1910, 2362
NgoMIV	GCCGGC	1 cut(s)	825
NlaIII	CATG	5 cut(s)	263, 1015, 1501, 2007, 2045
NlaIV	GGNNCC	4 cut(s)	824, 1278, 1471, 1731
NmuCI	GTSAC	5 cut(s)	70, 1688, 1699, 1941, 2378
NsiI	ATGCAT	1 cut(s)	925
NspI	RCATGY	1 cut(s)	2007
PaeR7I	CTCGAG	1 cut(s)	1903
PagI	TCATGA	2 cut(s)	259, 2041
PaqCI	CACCTGC	1 cut(s)	2078
PciSI	GCTCTTC	1 cut(s)	1275
PctI	GAATGC	4 cut(s)	837, 925, 949, 1759
PdiI	GCCGGC	1 cut(s)	827
PdmI	GAANNNNTTC	1 cut(s)	1320
PfeI	GAWTC	6 cut(s)	607, 643, 1291, 1316, 2252, 2339
PflFI	GACNNNGTC	1 cut(s)	887
PfoI	TCCNGGA	1 cut(s)	2255
PkrI	GCNGC	4 cut(s)	736, 1654, 1936, 2149
PleI	GAGTC	2 cut(s)	178, 2375
PluTI	GGCGCC	2 cut(s)	826, 1473
PpsI	GAGTC	2 cut(s)	178, 2375
PshBI	ATTAAT	3 cut(s)	122, 585, 1817
Psp124BI	GAGCTC	1 cut(s)	1056
PspFI	CCCAGC	1 cut(s)	2370
PspN4I	GGNNCC	4 cut(s)	824, 1278, 1471, 1731
PstNI	CAGNNNCTG	1 cut(s)	965
PsuI	RGATCY	2 cut(s)	805, 1760
PsyI	GACNNNGTC	1 cut(s)	887
RsaI	GTAC	7 cut(s)	361, 391, 753, 992, 1260, 1731, 2207
RsaNI	GTAC	7 cut(s)	360, 390, 752, 991, 1259, 1730, 2206
SacI	GAGCTC	1 cut(s)	1056
SacII	CCGCGG	1 cut(s)	735
SapI	GCTCTTC	1 cut(s)	1275
SatI	GCNGC	4 cut(s)	735, 1653, 1935, 2148
Sau3AI	GATC	7 cut(s)	262, 805, 1060, 1203, 1760, 1910, 2362
ScaI	AGTACT	2 cut(s)	391, 1260
SchI	GAGTC	2 cut(s)	179, 2375
ScrFI	CCNGG	1 cut(s)	2257
SduI	GDGCHC	2 cut(s)	291, 1056
SfaNI	GCATC	5 cut(s)	1224, 1232, 1341, 2014, 2271
SfoI	GGCGCC	2 cut(s)	824, 1471
Sfr274I	CTCGAG	1 cut(s)	1903
Sfr303I	CCGCGG	1 cut(s)	735
SgrBI	CCGCGG	1 cut(s)	735
SlaI	CTCGAG	1 cut(s)	1903
SmlI	CTYRAG	6 cut(s)	188, 1042, 1531, 1903, 2019, 2271
SmoI	CTYRAG	6 cut(s)	188, 1042, 1531, 1903, 2019, 2271
SpeI	ACTAGT	1 cut(s)	392
SsiI	CCGC	6 cut(s)	364, 732, 734, 1149, 1653, 1775
SspDI	GGCGCC	2 cut(s)	822, 1469
SspI	AATATT	2 cut(s)	1559, 2115
SspMI	CTAG	7 cut(s)	393, 930, 983, 1946, 2366, 2423, 2445
SstI	GAGCTC	1 cut(s)	1056
StyD4I	CCNGG	1 cut(s)	2255
StyI	CCWWGG	2 cut(s)	198, 1994
TaaI	ACNGT	2 cut(s)	463, 1200
TaiI	ACGT	1 cut(s)	1943
TaqI	TCGA	7 cut(s)	661, 996, 1069, 1134, 1904, 1985, 2250
TatI	WGTACW	3 cut(s)	389, 1258, 2205
TauI	GCSGC	2 cut(s)	737, 1655
TfiI	GAWTC	6 cut(s)	607, 643, 1291, 1316, 2252, 2339
TscAI	CASTG	5 cut(s)	1063, 1531, 2157, 2358, 2407
TseFI	GTSAC	5 cut(s)	70, 1688, 1699, 1941, 2378
TseI	GCWGC	2 cut(s)	1934, 2147
Tsp45I	GTSAC	5 cut(s)	70, 1688, 1699, 1941, 2378
TspGWI	ACGGA	3 cut(s)	1718, 2211, 2302
TspRI	CASTG	5 cut(s)	1063, 1531, 2157, 2358, 2407
Tth111I	GACNNNGTC	1 cut(s)	887
Vha464I	CTTAAG	1 cut(s)	188
VspI	ATTAAT	3 cut(s)	122, 585, 1817
XapI	RAATTY	9 cut(s)	54, 77, 226, 524, 1330, 1723, 1767, 1963, 2226
XceI	RCATGY	1 cut(s)	2007
XhoI	CTCGAG	1 cut(s)	1903
XmiI	GTMKAC	2 cut(s)	558, 655
XmnI	GAANNNNTTC	1 cut(s)	1320
XspI	CTAG	7 cut(s)	393, 930, 983, 1946, 2366, 2423, 2445
ZrmI	AGTACT	2 cut(s)	391, 1260
Zsp2I	ATGCAT	1 cut(s)	925

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.