pycom02g26610

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005122
Physical Location & Seq
Reverse (-)
24415044 .. 24417069
2026 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 993 bp
ATGGCGAAGTCTAAGAACAACAGCAATACCAAGAAACTTTCTTACATTTCGGTCCCTTCTCAGATTATCAACTCTCTGTCTCCGTCTTCTCTGCAATCGCTCCTCGTTTCTCCCAAGAAATCTTCAAGGAGCAAAAGCAGCAGCAGGTACTTGAGCTGGCTCAGCAACTACAGGAGCCCAAACTTCTGGGTTTTCTTCCTCTTCCTCTTTGGATTTCTTGGGATGCTGAAGCTGGGATTCCATATCGACCGTTTGATTCCCTATTCTCCATACCCATGTTCCACAACTCAGCTCCGGGACTTGGTCTCTAATGGCTACTTAAAATCACACATGGGTTTTGCTTCAAACGCTGAAAACAGCCACCAAGTCGAGGTTGTGAGCGAATCCCAGCCGTTGATTTCGAGTGGGGATTTGGAATCAGAGGTGGGGGTTGAGCTAAACAGTGGGAAAGTTGGGGAGGAAGCTGAGGGGAGTGAGTTTTGGAAGCAGCCGGATGGTTTGGGGTATCGGCCGTGCTTGGATTTGACGCAAGAGTACAAGAGAGCTAGTGAGGGGATAGTGAAGGACAGGACTAAATACCTGATTGTTGTGGTTTCTGGTGGGATGAACCAGCAGAGGAATCAGATTGTCGATGCTGTCGTTATTGCTAGGATTCTTGGTGCTGCTCTGGTGGTTCCTATTTTGCAAGTTAATGTCATCTGGGGGGATGAAAGTGAATTTTCTGATATATTTGATTTGGAGCACTTTAAGGAAGTCCTTGCTGACGATGTTCGGGTAGTTTCTTCACTGCCATCAACGCATCTAATGACTAGGCCTGTGGAGGAGAAGCGGACTCCTCTTCATGCTTCGCCTAATTGGATTCGGTCGCGTTATCACAAGAGGATTAAAAGAGAAGGGGTTTTACTGCTAAGAGGCTTGGATTCAAGGCTCTCTAAGGATCTTCTGACTCTGATCTCTTCTTTGTTTGACTTCAGGTTGCGTTTCATGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

331

Amino Acids

37.26

Weight (kDa)

9.18

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 193 - 319 6.2e-32 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 135
AccII CGCG 1 cut(s) 868
AciI CCGC 1 cut(s) 829
AclWI GGATC 1 cut(s) 945
AcoI YGGCCR 1 cut(s) 509
AcsI RAATTY 1 cut(s) 716
AcuI CTGAAG 2 cut(s) 248, 955
AfaI GTAC 2 cut(s) 149, 536
AfiI CCNNNNNNNGG 2 cut(s) 301, 370
AgsI TTSAA 3 cut(s) 126, 345, 924
AluBI AGCT 6 cut(s) 156, 232, 292, 436, 464, 545
AluI AGCT 6 cut(s) 156, 232, 292, 436, 464, 545
Alw21I GWGCWC 1 cut(s) 744
Alw26I GTCTC 2 cut(s) 84, 310
AlwI GGATC 1 cut(s) 945
AoxI GGCC 2 cut(s) 509, 812
ApeKI GCWGC 4 cut(s) 138, 141, 487, 662
ApoI RAATTY 1 cut(s) 716
AspS9I GGNCC 1 cut(s) 52
AsuC2I CCSGG 1 cut(s) 296
AvaII GGWCC 1 cut(s) 52
BanII GRGCYC 1 cut(s) 179
BarI GAAGNNNNNNTAC 2 cut(s) 885, 917
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 744
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 4 cut(s) 150, 153, 499, 649
BccI CCATC 2 cut(s) 488, 799
BceAI ACGGC 2 cut(s) 376, 496
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 2 cut(s) 84, 310
BfaI CTAG 3 cut(s) 546, 648, 810
BfmI CTRYAG 1 cut(s) 169
BfuAI ACCTGC 1 cut(s) 135
BisI GCNGC 4 cut(s) 139, 142, 488, 663
BlpI GCTNAGC 1 cut(s) 161
BlsI GCNGC 4 cut(s) 140, 143, 489, 664
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 3 cut(s) 54, 176, 675
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 3 cut(s) 213, 622, 808
BpiI GAAGAC 1 cut(s) 78
Bpu10I CCTNAGC 1 cut(s) 465
Bpu1102I GCTNAGC 1 cut(s) 161
BpuEI CTTGAG 1 cut(s) 172
BpuMI CCSGG 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 310
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 370
BseGI GGATG 4 cut(s) 228, 499, 609, 712
BseLI CCNNNNNNNGG 2 cut(s) 301, 370
BseMII CTCAG 4 cut(s) 74, 175, 302, 456
BseRI GAGGAG 3 cut(s) 92, 825, 836
BseX3I CGGCCG 1 cut(s) 509
BseXI GCAGC 4 cut(s) 150, 153, 499, 649
BseYI CCCAGC 2 cut(s) 232, 387
Bsh1236I CGCG 1 cut(s) 868
Bsh1285I CGRYCG 3 cut(s) 250, 512, 866
BshFI GGCC 2 cut(s) 511, 814
BsiEI CGRYCG 3 cut(s) 250, 512, 866
BsiHKAI GWGCWC 1 cut(s) 744
BsiSI CCGG 2 cut(s) 295, 491
BslFI GGGAC 2 cut(s) 38, 311
BslI CCNNNNNNNGG 2 cut(s) 301, 370
BsmAI GTCTC 2 cut(s) 84, 310
BsmFI GGGAC 2 cut(s) 38, 311
BsnI GGCC 2 cut(s) 511, 814
Bso31I GGTCTC 1 cut(s) 310
Bsp1286I GDGCHC 2 cut(s) 179, 744
Bsp143I GATC 2 cut(s) 937, 951
Bsp1720I GCTNAGC 1 cut(s) 161
BspACI CCGC 1 cut(s) 829
BspANI GGCC 2 cut(s) 511, 814
BspCNI CTCAG 4 cut(s) 73, 174, 301, 457
BspFNI CGCG 1 cut(s) 868
BspLI GGNNCC 3 cut(s) 54, 176, 675
BspMI ACCTGC 1 cut(s) 135
BspPI GGATC 1 cut(s) 945
BspTNI GGTCTC 1 cut(s) 310
BssMI GATC 2 cut(s) 937, 951
Bst4CI ACNGT 2 cut(s) 251, 443
Bst6I CTCTTC 3 cut(s) 206, 843, 961
BstC8I GCNNGC 1 cut(s) 158
BstDEI CTNAG 7 cut(s) 12, 60, 161, 288, 465, 908, 933
BstF5I GGATG 4 cut(s) 228, 499, 609, 712
BstFNI CGCG 1 cut(s) 868
BstKTI GATC 2 cut(s) 940, 954
BstMAI GTCTC 2 cut(s) 84, 310
BstMBI GATC 2 cut(s) 937, 951
BstMCI CGRYCG 3 cut(s) 250, 512, 866
BstMWI GCNNNNNNNGC 5 cut(s) 138, 162, 347, 796, 985
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 1 cut(s) 169
BstUI CGCG 1 cut(s) 868
BstV1I GCAGC 4 cut(s) 150, 153, 499, 649
BstV2I GAAGAC 1 cut(s) 78
BstX2I RGATCY 1 cut(s) 937
BstXI CCANNNNNNTGG 1 cut(s) 186
BstYI RGATCY 1 cut(s) 937
BstZI CGGCCG 1 cut(s) 509
BsuRI GGCC 2 cut(s) 511, 814
BtsCI GGATG 4 cut(s) 228, 499, 609, 712
BtsI GCAGTG 1 cut(s) 785
BtsIMutI CAGTG 2 cut(s) 448, 785
BveI ACCTGC 1 cut(s) 135
Cac8I GCNNGC 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 52
CseI GACGC 1 cut(s) 535
Csp6I GTAC 2 cut(s) 148, 535
CviAII CATG 4 cut(s) 276, 331, 842, 985
CviQI GTAC 2 cut(s) 148, 535
DdeI CTNAG 7 cut(s) 12, 60, 161, 288, 465, 908, 933
DpnI GATC 2 cut(s) 939, 953
DpnII GATC 2 cut(s) 937, 951
EaeI YGGCCR 1 cut(s) 509
EagI CGGCCG 1 cut(s) 509
Eam1104I CTCTTC 3 cut(s) 206, 843, 961
EarI CTCTTC 3 cut(s) 206, 843, 961
EclXI CGGCCG 1 cut(s) 509
Eco147I AGGCCT 1 cut(s) 814
Eco24I GRGCYC 1 cut(s) 179
Eco31I GGTCTC 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 52
Eco52I CGGCCG 1 cut(s) 509
Eco57I CTGAAG 2 cut(s) 248, 955
EcoT22I ATGCAT 1 cut(s) 990
EcoT38I GRGCYC 1 cut(s) 179
FaeI CATG 4 cut(s) 279, 334, 845, 988
FaiI YATR 7 cut(s) 243, 271, 277, 332, 728, 843, 986
FaqI GGGAC 2 cut(s) 38, 311
FatI CATG 4 cut(s) 275, 330, 841, 984
Fnu4HI GCNGC 4 cut(s) 139, 142, 488, 663
FokI GGATG 4 cut(s) 235, 506, 616, 719
FriOI GRGCYC 1 cut(s) 179
Fsp4HI GCNGC 4 cut(s) 139, 142, 488, 663
FspBI CTAG 3 cut(s) 546, 648, 810
GluI GCNGC 4 cut(s) 139, 142, 488, 663
GsaI CCCAGC 2 cut(s) 236, 391
HaeIII GGCC 2 cut(s) 511, 814
HapII CCGG 2 cut(s) 295, 491
HgaI GACGC 1 cut(s) 535
Hin1II CATG 4 cut(s) 279, 334, 845, 988
HpaII CCGG 2 cut(s) 295, 491
Hpy188I TCNGA 6 cut(s) 63, 421, 624, 724, 945, 951
HpyAV CCTTC 3 cut(s) 66, 556, 887
HpyCH4III ACNGT 2 cut(s) 251, 443
HpyCH4V TGCA 3 cut(s) 94, 685, 988
HpyF10VI GCNNNNNNNGC 5 cut(s) 138, 162, 347, 796, 985
HpyF3I CTNAG 7 cut(s) 12, 60, 161, 288, 465, 908, 933
Hsp92II CATG 4 cut(s) 279, 334, 845, 988
Kzo9I GATC 2 cut(s) 937, 951
LmnI GCTCC 5 cut(s) 105, 129, 174, 297, 739
Lsp1109I GCAGC 4 cut(s) 150, 153, 499, 649
LweI GCATC 3 cut(s) 213, 622, 808
MaeI CTAG 3 cut(s) 546, 648, 810
MalI GATC 2 cut(s) 939, 953
MboI GATC 2 cut(s) 937, 951
MboII GAAGA 8 cut(s) 78, 114, 187, 193, 774, 830, 932, 948
MflI RGATCY 1 cut(s) 937
MhlI GDGCHC 2 cut(s) 179, 744
MluCI AATT 2 cut(s) 716, 853
MlyI GAGTC 2 cut(s) 826, 940
Mph1103I ATGCAT 1 cut(s) 990
MseI TTAA 4 cut(s) 320, 690, 747, 885
MslI CAYNNNNRTG 1 cut(s) 274
MspI CCGG 2 cut(s) 295, 491
MspR9I CCNGG 1 cut(s) 296
MvnI CGCG 1 cut(s) 868
MwoI GCNNNNNNNGC 5 cut(s) 138, 162, 347, 796, 985
NciI CCSGG 1 cut(s) 296
NdeII GATC 2 cut(s) 937, 951
NlaIII CATG 4 cut(s) 279, 334, 845, 988
NlaIV GGNNCC 3 cut(s) 54, 176, 675
NsiI ATGCAT 1 cut(s) 990
PceI AGGCCT 1 cut(s) 814
PcsI WCGNNNNNNNCGW 1 cut(s) 636
PfeI GAWTC 8 cut(s) 237, 256, 383, 416, 619, 652, 859, 920
PflFI GACNNNGTC 1 cut(s) 302
PfoI TCCNGGA 1 cut(s) 294
PkrI GCNGC 4 cut(s) 140, 143, 489, 664
PleI GAGTC 2 cut(s) 826, 940
PpsI GAGTC 2 cut(s) 826, 940
PspFI CCCAGC 2 cut(s) 232, 387
PspN4I GGNNCC 3 cut(s) 54, 176, 675
PspPI GGNCC 1 cut(s) 52
PsuI RGATCY 1 cut(s) 937
PsyI GACNNNGTC 1 cut(s) 302
RsaI GTAC 2 cut(s) 149, 536
RsaNI GTAC 2 cut(s) 148, 535
RseI CAYNNNNRTG 1 cut(s) 274
SaqAI TTAA 4 cut(s) 320, 690, 747, 885
SatI GCNGC 4 cut(s) 139, 142, 488, 663
Sau3AI GATC 2 cut(s) 937, 951
Sau96I GGNCC 1 cut(s) 52
SchI GAGTC 2 cut(s) 826, 940
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 2 cut(s) 179, 744
SfaNI GCATC 3 cut(s) 213, 622, 808
SfcI CTRYAG 1 cut(s) 169
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 274
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
Sse9I AATT 2 cut(s) 716, 853
SseBI AGGCCT 1 cut(s) 814
SsiI CCGC 1 cut(s) 829
SspMI CTAG 3 cut(s) 546, 648, 810
StuI AGGCCT 1 cut(s) 814
StyD4I CCNGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 251, 443
TaqI TCGA 4 cut(s) 246, 369, 401, 630
TaqII GACCGA 2 cut(s) 40, 852
TasI AATT 2 cut(s) 716, 853
TatI WGTACW 1 cut(s) 534
TfiI GAWTC 8 cut(s) 237, 256, 383, 416, 619, 652, 859, 920
Tru1I TTAA 4 cut(s) 320, 690, 747, 885
Tru9I TTAA 4 cut(s) 320, 690, 747, 885
TscAI CASTG 2 cut(s) 448, 792
TseI GCWGC 4 cut(s) 138, 141, 487, 662
TspDTI ATGAA 4 cut(s) 620, 723, 830, 973
TspGWI ACGGA 1 cut(s) 72
TspRI CASTG 2 cut(s) 448, 792
Tth111I GACNNNGTC 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 52
XapI RAATTY 1 cut(s) 716
XspI CTAG 3 cut(s) 546, 648, 810
Zsp2I ATGCAT 1 cut(s) 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.