pycom03g04510

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0018013
Physical Location & Seq
Reverse (-)
3506687 .. 3507580
894 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g04510.1

Sequence Viewer

Length: 894 bp
ATGCCAAAAACACACAAATCATCCATCCAATATAATAACCACCGTCGATCCCTAAATCAACGGCCAAAGATTCTTCGCCCCAAATCCTATAAAACCATCCCTTTTACCAAAATCCACCCAAAAAACCTGCCCTCCCTTCTTCATATCTCTTCTGAGAAAGAAAAGTCGTTACAGTCTCTTTTCAGTCCTCTCTGTTCTTCCGAATTTCCAATATTACCCCGAACTTCCATCGTATTAACACCAATGTCCTCGCCTTCCCCGATCCCAGTTTCGTCCCAACAGTCCTCTCACCAGCAATCCCAACCGTCCGGCACCAACCCACTCCCATTCCTCCGCCCCTTCCTCACGCGCATCTCCGCCGTATCCCGCCACGCGCTCTCCAACAGCCGCCCCTGGACCGAGCTCATCGACCGCACTGCCTTCACCAGACCCACCTCGCTCACCGATGCCGCCTCCCGGATCCGCAAGAACGCCGCCTACTTCCGCGTCAACTACCTCATCGTACTCGCAGTCGTACTCGCCTACTCCCTCATCTCCCACCCCTTCTCCCTCCTCACCCTCGTCGCCCTCGCCGGAGCATGGATCTTCCTCTACTCGCGCCGATCGTCGGAGCAGCCGCTCGTGATCCTCGGCCGCACGTTCTCCGACACCCAGGCGCTGTTCGGTCTGGGCGTGGCGACGCTAATCGCGATCTTGGTGACGAGCGTGCTGTCGCTGCTGCTAACGGCGGTGATGGTGGGAGTTGGGATCGTGTGTGTTCACGGCGCATTCAGAGATCCTGAGGATCTGTTCCTGGACGAAACGCAGCCGTTGGGTTCTGGCATCGCTTCGATTTTCGGTGGCGGGGCCCCTTCGTTTGCGTCGGCAGGTGCCAGCATGATGTCACGTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

298

Amino Acids

32.41

Weight (kDa)

10.54

Isoelectric Point (pI)

59.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 125 - 267 8.7e-47 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018013)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g39780
malus_domestica MD03G1056700.v1.1 MD11G1058500.v1.1
prunus_persica Prupe.6G048100_v2.0.a1
pyrus_communis pycom03g04510
rosa_chinensis RchiOBHm_Chr5g0071661
rosa_multiflora Rmu_sc0005745.1_g000036
rosa_roxburghii Rroxscaffold_1G00009510
rosa_rugosa Rorug05G0414100
rosa_samantha Rh5BG489600
rosa_wichuraiana Rw5G043670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 857
Acc36I ACCTGC 2 cut(s) 135, 857
AccB1I GGYRCC 2 cut(s) 311, 869
AccBSI CCGCTC 1 cut(s) 619
AccII CGCG 5 cut(s) 349, 374, 486, 598, 689
AclWI GGATC 9 cut(s) 42, 256, 454, 467, 590, 619, 755, 770, 792
AcoI YGGCCR 2 cut(s) 62, 631
AcsI RAATTY 1 cut(s) 203
AcvI CACGTG 1 cut(s) 887
AfaI GTAC 2 cut(s) 504, 516
AfiI CCNNNNNNNGG 3 cut(s) 456, 579, 607
AflIII ACRYGT 1 cut(s) 886
AjnI CCWGG 3 cut(s) 392, 651, 792
AluBI AGCT 1 cut(s) 403
AluI AGCT 1 cut(s) 403
Alw21I GWGCWC 1 cut(s) 405
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 9 cut(s) 42, 256, 454, 467, 590, 619, 755, 770, 792
AlwNI CAGNNNCTG 1 cut(s) 658
AoxI GGCC 3 cut(s) 62, 631, 846
ApaI GGGCCC 1 cut(s) 850
ApeKI GCWGC 4 cut(s) 613, 715, 718, 805
ApoI RAATTY 1 cut(s) 203
AspLEI GCGC 5 cut(s) 351, 376, 600, 658, 767
AspS9I GGNCC 3 cut(s) 396, 846, 847
AsuC2I CCSGG 1 cut(s) 457
AsuHPI GGTGA 6 cut(s) 281, 415, 433, 547, 709, 742
AvaII GGWCC 1 cut(s) 396
AxyI CCTNAGG 1 cut(s) 780
BaeGI GKGCMC 1 cut(s) 850
BamHI GGATCC 1 cut(s) 459
BanI GGYRCC 2 cut(s) 311, 869
BanII GRGCYC 2 cut(s) 405, 850
BauI CACGAG 1 cut(s) 620
BbrPI CACGTG 1 cut(s) 887
Bbv12I GWGCWC 1 cut(s) 405
BbvI GCAGC 4 cut(s) 625, 702, 705, 817
BccI CCATC 4 cut(s) 32, 104, 236, 727
BceAI ACGGC 5 cut(s) 77, 344, 741, 778, 793
BcgI CGANNNNNNTGC 2 cut(s) 398, 432
BciT130I CCWGG 3 cut(s) 394, 653, 794
BciVI GTATCC 1 cut(s) 373
BcnI CCSGG 1 cut(s) 457
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 1 cut(s) 892
BfoI RGCGCY 1 cut(s) 659
BfuAI ACCTGC 2 cut(s) 135, 857
BfuI GTATCC 1 cut(s) 373
BisI GCNGC 9 cut(s) 388, 450, 474, 614, 617, 634, 716, 719, 806
BlsI GCNGC 9 cut(s) 389, 451, 475, 615, 618, 635, 717, 720, 807
Bme1390I CCNGG 4 cut(s) 394, 457, 653, 794
Bme18I GGWCC 1 cut(s) 396
BmgT120I GGNCC 3 cut(s) 396, 846, 847
BmiI GGNNCC 6 cut(s) 313, 461, 847, 848, 849, 871
BmrFI CCNGG 4 cut(s) 394, 457, 653, 794
BmrI ACTGGG 1 cut(s) 260
BmsI GCATC 3 cut(s) 360, 436, 831
BmuI ACTGGG 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 457
BsaAI YACGTR 1 cut(s) 887
BsaJI CCNNGG 3 cut(s) 392, 628, 651
BsaXI ACNNNNNCTCC 6 cut(s) 116, 146, 362, 392, 530, 560
Bsc4I CCNNNNNNNGG 3 cut(s) 456, 579, 607
Bse1I ACTGG 1 cut(s) 266
Bse21I CCTNAGG 1 cut(s) 780
BseBI CCWGG 3 cut(s) 394, 653, 794
BseDI CCNNGG 3 cut(s) 392, 628, 651
BseGI GGATG 3 cut(s) 20, 24, 96
BseLI CCNNNNNNNGG 3 cut(s) 456, 579, 607
BseMII CTCAG 2 cut(s) 144, 771
BseNI ACTGG 1 cut(s) 266
BseRI GAGGAG 1 cut(s) 542
BseSI GKGCMC 1 cut(s) 850
BseX3I CGGCCG 1 cut(s) 631
BseXI GCAGC 4 cut(s) 625, 702, 705, 817
Bsh1236I CGCG 5 cut(s) 349, 374, 486, 598, 689
Bsh1285I CGRYCG 3 cut(s) 412, 605, 634
BshFI GGCC 3 cut(s) 64, 633, 848
BshNI GGYRCC 2 cut(s) 311, 869
BsiEI CGRYCG 3 cut(s) 412, 605, 634
BsiHKAI GWGCWC 1 cut(s) 405
BsiSI CCGG 3 cut(s) 309, 457, 573
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 3 cut(s) 456, 579, 607
BsmAI GTCTC 1 cut(s) 180
BsmFI GGGAC 1 cut(s) 259
BsmI GAATGC 1 cut(s) 767
BsnI GGCC 3 cut(s) 64, 633, 848
Bsp120I GGGCCC 1 cut(s) 846
Bsp1286I GDGCHC 2 cut(s) 405, 850
Bsp68I TCGCGA 1 cut(s) 689
BspANI GGCC 3 cut(s) 64, 633, 848
BspCNI CTCAG 2 cut(s) 145, 772
BspFNI CGCG 5 cut(s) 349, 374, 486, 598, 689
BspLI GGNNCC 6 cut(s) 313, 461, 847, 848, 849, 871
BspMI ACCTGC 2 cut(s) 135, 857
BspPI GGATC 9 cut(s) 42, 256, 454, 467, 590, 619, 755, 770, 792
BspT107I GGYRCC 2 cut(s) 311, 869
BsrBI CCGCTC 1 cut(s) 619
BsrI ACTGG 1 cut(s) 266
BssECI CCNNGG 3 cut(s) 392, 628, 651
BssSI CACGAG 1 cut(s) 620
Bst2BI CACGAG 1 cut(s) 620
Bst2UI CCWGG 3 cut(s) 394, 653, 794
Bst4CI ACNGT 4 cut(s) 44, 174, 282, 306
Bst6I CTCTTC 1 cut(s) 154
BstBAI YACGTR 1 cut(s) 887
BstC8I GCNNGC 2 cut(s) 707, 874
BstDEI CTNAG 2 cut(s) 153, 780
BstF5I GGATG 3 cut(s) 20, 24, 96
BstFNI CGCG 5 cut(s) 349, 374, 486, 598, 689
BstH2I RGCGCY 1 cut(s) 659
BstHHI GCGC 5 cut(s) 351, 376, 600, 658, 767
BstMAI GTCTC 1 cut(s) 180
BstMCI CGRYCG 3 cut(s) 412, 605, 634
BstMWI GCNNNNNNNGC 1 cut(s) 715
BstNI CCWGG 3 cut(s) 394, 653, 794
BstSCI CCNGG 4 cut(s) 392, 455, 651, 792
BstSLI GKGCMC 1 cut(s) 850
BstUI CGCG 5 cut(s) 349, 374, 486, 598, 689
BstV1I GCAGC 4 cut(s) 625, 702, 705, 817
BstX2I RGATCY 4 cut(s) 459, 582, 775, 784
BstYI RGATCY 4 cut(s) 459, 582, 775, 784
BstZI CGGCCG 1 cut(s) 631
Bsu36I CCTNAGG 1 cut(s) 780
BsuI GTATCC 1 cut(s) 373
BsuRI GGCC 3 cut(s) 64, 633, 848
BtgZI GCGATG 1 cut(s) 808
BtsCI GGATG 3 cut(s) 20, 24, 96
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 1 cut(s) 414
BtuMI TCGCGA 1 cut(s) 689
BveI ACCTGC 2 cut(s) 135, 857
Cac8I GCNNGC 2 cut(s) 707, 874
CaiI CAGNNNCTG 1 cut(s) 658
CfoI GCGC 5 cut(s) 351, 376, 600, 658, 767
Cfr13I GGNCC 3 cut(s) 396, 846, 847
CseI GACGC 3 cut(s) 475, 688, 849
Csp6I GTAC 2 cut(s) 503, 515
CviAII CATG 2 cut(s) 579, 877
CviJI RGCY 7 cut(s) 64, 387, 403, 616, 633, 808, 848
CviKI_1 RGCY 7 cut(s) 64, 387, 403, 616, 633, 808, 848
CviQI GTAC 2 cut(s) 503, 515
DdeI CTNAG 2 cut(s) 153, 780
EaeI YGGCCR 2 cut(s) 62, 631
EagI CGGCCG 1 cut(s) 631
Eam1104I CTCTTC 1 cut(s) 154
EarI CTCTTC 1 cut(s) 154
EciI GGCGGA 2 cut(s) 323, 346
Ecl136II GAGCTC 1 cut(s) 403
EclXI CGGCCG 1 cut(s) 631
Eco24I GRGCYC 2 cut(s) 405, 850
Eco47I GGWCC 1 cut(s) 396
Eco52I CGGCCG 1 cut(s) 631
Eco53kI GAGCTC 1 cut(s) 403
Eco72I CACGTG 1 cut(s) 887
Eco81I CCTNAGG 1 cut(s) 780
EcoICRI GAGCTC 1 cut(s) 403
EcoO109I RGGNCCY 2 cut(s) 846, 847
EcoRII CCWGG 3 cut(s) 392, 651, 792
EcoT38I GRGCYC 2 cut(s) 405, 850
FaeI CATG 2 cut(s) 582, 880
FaiI YATR 5 cut(s) 33, 90, 144, 580, 878
FaqI GGGAC 1 cut(s) 259
FatI CATG 2 cut(s) 578, 876
FauI CCCGC 2 cut(s) 374, 836
Fnu4HI GCNGC 9 cut(s) 388, 450, 474, 614, 617, 634, 716, 719, 806
FokI GGATG 3 cut(s) 7, 11, 83
FriOI GRGCYC 2 cut(s) 405, 850
Fsp4HI GCNGC 9 cut(s) 388, 450, 474, 614, 617, 634, 716, 719, 806
FspBI CTAG 1 cut(s) 892
GlaI GCGC 5 cut(s) 350, 375, 599, 657, 766
GluI GCNGC 9 cut(s) 388, 450, 474, 614, 617, 634, 716, 719, 806
HaeII RGCGCY 1 cut(s) 659
HaeIII GGCC 3 cut(s) 64, 633, 848
HapII CCGG 3 cut(s) 309, 457, 573
HgaI GACGC 3 cut(s) 475, 688, 849
HhaI GCGC 5 cut(s) 351, 376, 600, 658, 767
Hin1II CATG 2 cut(s) 582, 880
Hin6I GCGC 5 cut(s) 349, 374, 598, 656, 765
HinP1I GCGC 5 cut(s) 349, 374, 598, 656, 765
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HinfI GANTC 1 cut(s) 70
HpaII CCGG 3 cut(s) 309, 457, 573
HphI GGTGA 6 cut(s) 281, 415, 433, 547, 709, 742
Hpy166II GTNNAC 2 cut(s) 490, 760
Hpy188I TCNGA 5 cut(s) 154, 202, 610, 646, 773
Hpy188III TCNNGA 3 cut(s) 622, 688, 779
Hpy8I GTNNAC 2 cut(s) 490, 760
Hpy99I CGWCG 5 cut(s) 48, 566, 610, 682, 865
HpyAV CCTTC 6 cut(s) 146, 264, 349, 430, 553, 861
HpyCH4III ACNGT 4 cut(s) 44, 174, 282, 306
HpyCH4IV ACGT 2 cut(s) 638, 886
HpyF10VI GCNNNNNNNGC 1 cut(s) 715
HpyF3I CTNAG 2 cut(s) 153, 780
HpySE526I ACGT 2 cut(s) 638, 886
Hsp92II CATG 2 cut(s) 582, 880
HspAI GCGC 5 cut(s) 349, 374, 598, 656, 765
LmnI GCTCC 2 cut(s) 575, 610
Lsp1109I GCAGC 4 cut(s) 625, 702, 705, 817
LweI GCATC 3 cut(s) 360, 436, 831
MaeI CTAG 1 cut(s) 892
MaeII ACGT 2 cut(s) 638, 886
MaeIII GTNAC 3 cut(s) 168, 697, 882
MbiI CCGCTC 1 cut(s) 619
MboII GAAGA 5 cut(s) 65, 131, 141, 189, 577
MflI RGATCY 4 cut(s) 459, 582, 775, 784
MhlI GDGCHC 2 cut(s) 405, 850
MluCI AATT 1 cut(s) 203
MmeI TCCRAC 3 cut(s) 405, 588, 669
MseI TTAA 1 cut(s) 236
MspI CCGG 3 cut(s) 309, 457, 573
MspR9I CCNGG 4 cut(s) 394, 457, 653, 794
Mva1269I GAATGC 1 cut(s) 767
MvaI CCWGG 3 cut(s) 394, 653, 794
MvnI CGCG 5 cut(s) 349, 374, 486, 598, 689
MwoI GCNNNNNNNGC 1 cut(s) 715
NciI CCSGG 1 cut(s) 457
NlaIII CATG 2 cut(s) 582, 880
NlaIV GGNNCC 6 cut(s) 313, 461, 847, 848, 849, 871
NmeAIII GCCGAG 1 cut(s) 609
NmuCI GTSAC 2 cut(s) 697, 882
NruI TCGCGA 1 cut(s) 689
PaqCI CACCTGC 1 cut(s) 857
PcsI WCGNNNNNNNCGW 4 cut(s) 257, 602, 669, 686
PctI GAATGC 1 cut(s) 767
PfeI GAWTC 1 cut(s) 70
PfoI TCCNGGA 2 cut(s) 455, 792
PkrI GCNGC 9 cut(s) 389, 451, 475, 615, 618, 635, 717, 720, 807
Ple19I CGATCG 1 cut(s) 605
PmaCI CACGTG 1 cut(s) 887
PmlI CACGTG 1 cut(s) 887
Ppu21I YACGTR 1 cut(s) 887
Psp124BI GAGCTC 1 cut(s) 405
Psp6I CCWGG 3 cut(s) 392, 651, 792
PspCI CACGTG 1 cut(s) 887
PspGI CCWGG 3 cut(s) 392, 651, 792
PspN4I GGNNCC 6 cut(s) 313, 461, 847, 848, 849, 871
PspOMI GGGCCC 1 cut(s) 846
PspPI GGNCC 3 cut(s) 396, 846, 847
PsrI GAACNNNNNNTAC 2 cut(s) 461, 493
PstNI CAGNNNCTG 1 cut(s) 658
PsuI RGATCY 4 cut(s) 459, 582, 775, 784
PvuI CGATCG 1 cut(s) 605
RruI TCGCGA 1 cut(s) 689
RsaI GTAC 2 cut(s) 504, 516
RsaNI GTAC 2 cut(s) 503, 515
SacI GAGCTC 1 cut(s) 405
SaqAI TTAA 1 cut(s) 236
SatI GCNGC 9 cut(s) 388, 450, 474, 614, 617, 634, 716, 719, 806
Sau96I GGNCC 3 cut(s) 396, 846, 847
ScrFI CCNGG 4 cut(s) 394, 457, 653, 794
SduI GDGCHC 2 cut(s) 405, 850
SetI ASST 7 cut(s) 129, 405, 437, 498, 641, 871, 889
SfaNI GCATC 3 cut(s) 360, 436, 831
SinI GGWCC 1 cut(s) 396
Sse9I AATT 1 cut(s) 203
SspI AATATT 1 cut(s) 213
SspMI CTAG 1 cut(s) 892
SstI GAGCTC 1 cut(s) 405
StyD4I CCNGG 4 cut(s) 392, 455, 651, 792
TaaI ACNGT 4 cut(s) 44, 174, 282, 306
TaiI ACGT 2 cut(s) 641, 889
TaqI TCGA 3 cut(s) 46, 408, 830
TaqII GACCGA 2 cut(s) 413, 653
TasI AATT 1 cut(s) 203
TauI GCSGC 5 cut(s) 390, 452, 476, 619, 636
TfiI GAWTC 1 cut(s) 70
Tru1I TTAA 1 cut(s) 236
Tru9I TTAA 1 cut(s) 236
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 2 cut(s) 697, 882
TseI GCWGC 4 cut(s) 613, 715, 718, 805
Tsp45I GTSAC 2 cut(s) 697, 882
TspDTI ATGAA 1 cut(s) 131
TspRI CASTG 1 cut(s) 421
VpaK11BI GGWCC 1 cut(s) 396
XapI RAATTY 1 cut(s) 203
XspI CTAG 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.