Rw5G043670

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0018013
Physical Location & Seq
Reverse (-)
76593534 .. 76594593
1060 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G043670.1

Sequence Viewer

Length: 639 bp
ATGTCCTCGTCTTCCTCGATCCCGATGTCCTCCTCCTCCTCTTCGCAACAGTCCACACAGCCACCCTCATTTCCGCTGCCGCCGTTCCGATCCATCCTCACGCGCCTCTCCCGCTTCTCCCGCAACGCGCTCGTCAACCAACGCCCCTGGACCGAGCTCGTCGACCGCACCGCCTTCACCCGACCGTCCTCCTTCTCCGACGCGGCCTCGCGCGTGAGGAAAAACGCCGCCTACTTCCGCGTCAACTACCTGATCGTGCTCGCCGTCGTCCTCGCCTACTCACTCCTCTCCCACCCCTTCACTCTCCTCACCCTCGTCGGCCTCGCCGCCGCGTGGATCTTCCTCTACTCGCACCGACAGTCGGATCAGCCGCTCGTGATCTTGGGCCGGACCTACTCCGACACGCAGGTGCTGTTTGGGCTGGGCCTGGTGACGCTGATCGCGATCTTGATGACGAGCGTGTTGTCGCTGCTCATAACGGCGCTGATGGTGGGAGTCGGGATCGTCTGTGCTCACGGCGCGTTTAGGGATCCTGAGGATCTGTTCTTGGATGAATCGCAGCCGTTGGGTTCTGGATTCGCTTCGATGTTCAGTGGGGCCGCTTCCTCTGCTGGCGCCAGCATGGTGTCACGTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

212

Amino Acids

22.87

Weight (kDa)

9.99

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 44 - 185 1.8e-47 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018013)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g39780
malus_domestica MD03G1056700.v1.1 MD11G1058500.v1.1
prunus_persica Prupe.6G048100_v2.0.a1
pyrus_communis pycom03g04510
rosa_chinensis RchiOBHm_Chr5g0071661
rosa_multiflora Rmu_sc0005745.1_g000036
rosa_roxburghii Rroxscaffold_1G00009510
rosa_rugosa Rorug05G0414100
rosa_samantha Rh5BG489600
rosa_wichuraiana Rw5G043670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 397
Acc36I ACCTGC 1 cut(s) 397
AccB1I GGYRCC 1 cut(s) 614
AccBSI CCGCTC 1 cut(s) 373
AccI GTMKAC 1 cut(s) 162
AccII CGCG 9 cut(s) 103, 128, 203, 211, 213, 240, 332, 443, 521
AclWI GGATC 8 cut(s) 13, 84, 344, 372, 509, 524, 537, 546
AcvI CACGTG 1 cut(s) 632
AcyI GRCGYC 1 cut(s) 615
AfiI CCNNNNNNNGG 2 cut(s) 333, 361
AflIII ACRYGT 1 cut(s) 631
AjnI CCWGG 2 cut(s) 146, 426
AleI CACNNNNGTG 1 cut(s) 407
AluBI AGCT 1 cut(s) 157
AluI AGCT 1 cut(s) 157
Alw21I GWGCWC 3 cut(s) 159, 261, 514
AlwI GGATC 8 cut(s) 13, 84, 344, 372, 509, 524, 537, 546
AlwNI CAGNNNCTG 1 cut(s) 412
AoxI GGCC 5 cut(s) 204, 319, 385, 424, 597
ApeKI GCWGC 3 cut(s) 76, 469, 559
AspLEI GCGC 6 cut(s) 105, 130, 213, 484, 521, 617
AspS9I GGNCC 5 cut(s) 150, 385, 390, 424, 597
AsuHPI GGTGA 3 cut(s) 169, 301, 442
AvaII GGWCC 2 cut(s) 150, 390
AxyI CCTNAGG 1 cut(s) 534
BamHI GGATCC 1 cut(s) 529
BanI GGYRCC 1 cut(s) 614
BanII GRGCYC 1 cut(s) 159
BauI CACGAG 1 cut(s) 374
BbrPI CACGTG 1 cut(s) 632
BbsI GAAGAC 1 cut(s) 3
Bbv12I GWGCWC 3 cut(s) 159, 261, 514
BbvI GCAGC 3 cut(s) 63, 456, 571
BccI CCATC 2 cut(s) 101, 481
BceAI ACGGC 5 cut(s) 67, 248, 495, 532, 547
BcgI CGANNNNNNTGC 2 cut(s) 112, 146
BciT130I CCWGG 2 cut(s) 148, 428
BfaI CTAG 1 cut(s) 637
BfoI RGCGCY 2 cut(s) 485, 618
BfuAI ACCTGC 1 cut(s) 397
Bme1390I CCNGG 2 cut(s) 148, 428
Bme18I GGWCC 2 cut(s) 150, 390
BmgT120I GGNCC 5 cut(s) 150, 385, 390, 424, 597
BmiI GGNNCC 3 cut(s) 531, 598, 616
BmrFI CCNGG 2 cut(s) 148, 428
BpiI GAAGAC 1 cut(s) 3
BsaAI YACGTR 1 cut(s) 632
BsaBI GATNNNNATC 1 cut(s) 443
BsaHI GRCGYC 1 cut(s) 615
BsaJI CCNNGG 1 cut(s) 146
Bsc4I CCNNNNNNNGG 2 cut(s) 333, 361
Bse21I CCTNAGG 1 cut(s) 534
Bse8I GATNNNNATC 1 cut(s) 443
BseBI CCWGG 2 cut(s) 148, 428
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 2 cut(s) 93, 556
BseJI GATNNNNATC 1 cut(s) 443
BseLI CCNNNNNNNGG 2 cut(s) 333, 361
BseMII CTCAG 1 cut(s) 525
BseRI GAGGAG 5 cut(s) 22, 25, 28, 275, 296
BseXI GCAGC 3 cut(s) 63, 456, 571
BseYI CCCAGC 1 cut(s) 421
Bsh1236I CGCG 9 cut(s) 103, 128, 203, 211, 213, 240, 332, 443, 521
Bsh1285I CGRYCG 2 cut(s) 166, 185
BshFI GGCC 5 cut(s) 206, 321, 387, 426, 599
BshNI GGYRCC 1 cut(s) 614
BsiEI CGRYCG 2 cut(s) 166, 185
BsiHKAI GWGCWC 3 cut(s) 159, 261, 514
BsiSI CCGG 1 cut(s) 388
BslI CCNNNNNNNGG 2 cut(s) 333, 361
BsnI GGCC 5 cut(s) 206, 321, 387, 426, 599
Bsp1286I GDGCHC 3 cut(s) 159, 261, 514
Bsp68I TCGCGA 1 cut(s) 443
BspANI GGCC 5 cut(s) 206, 321, 387, 426, 599
BspCNI CTCAG 1 cut(s) 526
BspFNI CGCG 9 cut(s) 103, 128, 203, 211, 213, 240, 332, 443, 521
BspLI GGNNCC 3 cut(s) 531, 598, 616
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 8 cut(s) 13, 84, 344, 372, 509, 524, 537, 546
BspT107I GGYRCC 1 cut(s) 614
BsrBI CCGCTC 1 cut(s) 373
BssECI CCNNGG 1 cut(s) 146
BssNI GRCGYC 1 cut(s) 615
BssSI CACGAG 1 cut(s) 374
Bst2BI CACGAG 1 cut(s) 374
Bst2UI CCWGG 2 cut(s) 148, 428
Bst4CI ACNGT 3 cut(s) 51, 186, 360
Bst6I CTCTTC 1 cut(s) 46
BstACI GRCGYC 1 cut(s) 615
BstBAI YACGTR 1 cut(s) 632
BstC8I GCNNGC 3 cut(s) 261, 613, 619
BstDEI CTNAG 1 cut(s) 534
BstF5I GGATG 2 cut(s) 93, 556
BstFNI CGCG 9 cut(s) 103, 128, 203, 211, 213, 240, 332, 443, 521
BstH2I RGCGCY 2 cut(s) 485, 618
BstHHI GCGC 6 cut(s) 105, 130, 213, 484, 521, 617
BstMCI CGRYCG 2 cut(s) 166, 185
BstMWI GCNNNNNNNGC 5 cut(s) 111, 120, 418, 518, 608
BstNI CCWGG 2 cut(s) 148, 428
BstSCI CCNGG 2 cut(s) 146, 426
BstUI CGCG 9 cut(s) 103, 128, 203, 211, 213, 240, 332, 443, 521
BstV1I GCAGC 3 cut(s) 63, 456, 571
BstV2I GAAGAC 1 cut(s) 3
BstX2I RGATCY 3 cut(s) 336, 529, 538
BstYI RGATCY 3 cut(s) 336, 529, 538
Bsu36I CCTNAGG 1 cut(s) 534
BsuRI GGCC 5 cut(s) 206, 321, 387, 426, 599
BtsCI GGATG 2 cut(s) 93, 556
BtsIMutI CAGTG 1 cut(s) 598
BtuMI TCGCGA 1 cut(s) 443
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 3 cut(s) 261, 613, 619
CaiI CAGNNNCTG 1 cut(s) 412
CfoI GCGC 6 cut(s) 105, 130, 213, 484, 521, 617
Cfr13I GGNCC 5 cut(s) 150, 385, 390, 424, 597
CseI GACGC 3 cut(s) 209, 229, 442
CviAII CATG 1 cut(s) 622
DdeI CTNAG 1 cut(s) 534
DinI GGCGCC 1 cut(s) 616
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
Ecl136II GAGCTC 1 cut(s) 157
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 2 cut(s) 150, 390
Eco53kI GAGCTC 1 cut(s) 157
Eco72I CACGTG 1 cut(s) 632
Eco81I CCTNAGG 1 cut(s) 534
EcoICRI GAGCTC 1 cut(s) 157
EcoRII CCWGG 2 cut(s) 146, 426
EcoT38I GRGCYC 1 cut(s) 159
EgeI GGCGCC 1 cut(s) 616
EheI GGCGCC 1 cut(s) 616
FaeI CATG 1 cut(s) 625
FaiI YATR 2 cut(s) 476, 623
FatI CATG 1 cut(s) 621
FauI CCCGC 2 cut(s) 119, 128
FblI GTMKAC 1 cut(s) 162
FokI GGATG 2 cut(s) 80, 563
FriOI GRGCYC 1 cut(s) 159
FspBI CTAG 1 cut(s) 637
GlaI GCGC 6 cut(s) 104, 129, 212, 483, 520, 616
GsaI CCCAGC 1 cut(s) 425
HaeII RGCGCY 2 cut(s) 485, 618
HaeIII GGCC 5 cut(s) 206, 321, 387, 426, 599
HapII CCGG 1 cut(s) 388
HgaI GACGC 3 cut(s) 209, 229, 442
HhaI GCGC 6 cut(s) 105, 130, 213, 484, 521, 617
Hin1I GRCGYC 1 cut(s) 615
Hin1II CATG 1 cut(s) 625
Hin6I GCGC 6 cut(s) 103, 128, 211, 482, 519, 615
HinP1I GCGC 6 cut(s) 103, 128, 211, 482, 519, 615
HincII GTYRAC 3 cut(s) 136, 163, 244
HindII GTYRAC 3 cut(s) 136, 163, 244
HinfI GANTC 3 cut(s) 495, 554, 576
HpaII CCGG 1 cut(s) 388
HphI GGTGA 3 cut(s) 169, 301, 442
Hpy166II GTNNAC 4 cut(s) 54, 136, 163, 244
Hpy188I TCNGA 4 cut(s) 89, 199, 364, 400
Hpy188III TCNNGA 7 cut(s) 22, 376, 442, 448, 499, 533, 573
Hpy8I GTNNAC 4 cut(s) 54, 136, 163, 244
Hpy99I CGWCG 4 cut(s) 164, 203, 269, 320
HpyAV CCTTC 3 cut(s) 184, 202, 307
HpyCH4III ACNGT 3 cut(s) 51, 186, 360
HpyCH4IV ACGT 1 cut(s) 631
HpyF10VI GCNNNNNNNGC 5 cut(s) 111, 120, 418, 518, 608
HpyF3I CTNAG 1 cut(s) 534
HpySE526I ACGT 1 cut(s) 631
Hsp92I GRCGYC 1 cut(s) 615
Hsp92II CATG 1 cut(s) 625
HspAI GCGC 6 cut(s) 103, 128, 211, 482, 519, 615
KasI GGCGCC 1 cut(s) 614
Lsp1109I GCAGC 3 cut(s) 63, 456, 571
MaeI CTAG 1 cut(s) 637
MaeII ACGT 1 cut(s) 631
MaeIII GTNAC 2 cut(s) 430, 627
MbiI CCGCTC 1 cut(s) 373
MboII GAAGA 3 cut(s) 3, 33, 331
MflI RGATCY 3 cut(s) 336, 529, 538
MhlI GDGCHC 3 cut(s) 159, 261, 514
Mly113I GGCGCC 1 cut(s) 615
MlyI GAGTC 1 cut(s) 504
MmeI TCCRAC 3 cut(s) 222, 342, 423
MslI CAYNNNNRTG 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 76
MspI CCGG 1 cut(s) 388
MspR9I CCNGG 2 cut(s) 148, 428
MvaI CCWGG 2 cut(s) 148, 428
MvnI CGCG 9 cut(s) 103, 128, 203, 211, 213, 240, 332, 443, 521
MwoI GCNNNNNNNGC 5 cut(s) 111, 120, 418, 518, 608
NarI GGCGCC 1 cut(s) 615
NlaIII CATG 1 cut(s) 625
NlaIV GGNNCC 3 cut(s) 531, 598, 616
NmuCI GTSAC 2 cut(s) 430, 627
NruI TCGCGA 1 cut(s) 443
OliI CACNNNNGTG 1 cut(s) 407
PaqCI CACCTGC 1 cut(s) 397
PcsI WCGNNNNNNNCGW 3 cut(s) 14, 261, 440
PfeI GAWTC 2 cut(s) 554, 576
PleI GAGTC 1 cut(s) 503
PluTI GGCGCC 1 cut(s) 618
PmaCI CACGTG 1 cut(s) 632
PmlI CACGTG 1 cut(s) 632
PpsI GAGTC 1 cut(s) 503
Ppu21I YACGTR 1 cut(s) 632
Psp124BI GAGCTC 1 cut(s) 159
Psp6I CCWGG 2 cut(s) 146, 426
PspCI CACGTG 1 cut(s) 632
PspFI CCCAGC 1 cut(s) 421
PspGI CCWGG 2 cut(s) 146, 426
PspN4I GGNNCC 3 cut(s) 531, 598, 616
PspPI GGNCC 5 cut(s) 150, 385, 390, 424, 597
PstNI CAGNNNCTG 1 cut(s) 412
PsuI RGATCY 3 cut(s) 336, 529, 538
RruI TCGCGA 1 cut(s) 443
RseI CAYNNNNRTG 1 cut(s) 407
SacI GAGCTC 1 cut(s) 159
SalI GTCGAC 1 cut(s) 161
Sau96I GGNCC 5 cut(s) 150, 385, 390, 424, 597
SchI GAGTC 1 cut(s) 504
ScrFI CCNGG 2 cut(s) 148, 428
SduI GDGCHC 3 cut(s) 159, 261, 514
SetI ASST 5 cut(s) 159, 252, 395, 411, 634
SfoI GGCGCC 1 cut(s) 616
SinI GGWCC 2 cut(s) 150, 390
SmiMI CAYNNNNRTG 1 cut(s) 407
SspDI GGCGCC 1 cut(s) 614
SspMI CTAG 1 cut(s) 637
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 2 cut(s) 146, 426
TaaI ACNGT 3 cut(s) 51, 186, 360
TaiI ACGT 1 cut(s) 634
TaqI TCGA 3 cut(s) 17, 162, 584
TaqII GACCGA 1 cut(s) 167
TauI GCSGC 7 cut(s) 82, 206, 230, 329, 332, 373, 602
TfiI GAWTC 2 cut(s) 554, 576
TscAI CASTG 1 cut(s) 598
TseFI GTSAC 2 cut(s) 430, 627
TseI GCWGC 3 cut(s) 76, 469, 559
Tsp45I GTSAC 2 cut(s) 430, 627
TspDTI ATGAA 1 cut(s) 567
TspRI CASTG 1 cut(s) 598
VpaK11BI GGWCC 2 cut(s) 150, 390
XmiI GTMKAC 1 cut(s) 162
XspI CTAG 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.