pycom03g04920
ERF Family

Gamma-interferon-inducible lysosomal thiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007317
Physical Location & Seq
Forward (+)
3832740 .. 3833975
1236 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
ATGGCTTCCTCTTGGCTCGAAAGTAGAACCCTTTCTTTTTCTCTTCTGTTTTGCCTCTTCTTGGTTGATGTGTTCACACCCCCTTCATCTTCTGCAGACAGAACTCCGCCTTCGGATTCTGGTAAAGTTTCGCTGGCTTTGTATTACGAGTCCCCTTGCCCCTACAGCGCCAACTTCATTGTGAATTACCTTGTCAAGCTCTTCAAAGACGAGCTGATCTCCATTGTCGATCTCAAGCTTTCTCCTTGGGGTAATGCCAAGCTCAGAAGCAATGACACATTCACTTGCCAGCATGGTCCATCTGAATGTTTATTGAACACTGTGGAAGCCTGTGCAACTGAGATCTGGTCTGCACTGAATGATCTTTTCCTTTCGTTTATTGCGCCAAGAGTTTGGTTTATGAGCACAAGCAGCCCCAGTGGGAGTCATGTTACGAGAAACTGGGCTTGGATTCAAAGCCTATTACTGAATGCTACGGCAGTGGACATGCATGGTAAAGAGCTTGAGCTACAATATGCAGCTGAAACCAGTGCACTCCAGCCTCCGCATCAGTATGTGCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04612 map04612
Pfam Domains
Protein Families
GILT

Protein Analysis

188

Amino Acids

20.82

Weight (kDa)

5.07

Isoelectric Point (pI)

48.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GILT PF03227 45 - 121 6.1e-24 Gamma interferon inducible lysosomal thiol reductase (GILT)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 107, 545
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 3 cut(s) 205, 316, 455
AjuI GAANNNNNNNTTGG 2 cut(s) 430, 462
AluBI AGCT 7 cut(s) 199, 214, 238, 262, 502, 508, 521
AluI AGCT 7 cut(s) 199, 214, 238, 262, 502, 508, 521
Alw21I GWGCWC 2 cut(s) 407, 535
Alw44I GTGCAC 1 cut(s) 531
ApaLI GTGCAC 1 cut(s) 531
ApeKI GCWGC 2 cut(s) 411, 518
Asp700I GAANNNNTTC 1 cut(s) 31
AspLEI GCGC 2 cut(s) 170, 385
AspS9I GGNCC 1 cut(s) 296
AvaII GGWCC 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 535
Bbv12I GWGCWC 2 cut(s) 407, 535
BbvI GCAGC 2 cut(s) 423, 530
BccI CCATC 1 cut(s) 307
BceAI ACGGC 2 cut(s) 492, 544
BfmI CTRYAG 2 cut(s) 93, 163
BfoI RGCGCY 1 cut(s) 171
BglII AGATCT 1 cut(s) 342
BisI GCNGC 2 cut(s) 412, 519
BlsI GCNGC 2 cut(s) 413, 520
Bme18I GGWCC 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 296
BmrI ACTGGG 2 cut(s) 411, 451
BmsI GCATC 1 cut(s) 556
BmuI ACTGGG 2 cut(s) 411, 451
BplI GAGNNNNNCTC 2 cut(s) 203, 235
BpmI CTGGAG 1 cut(s) 521
BpuEI CTTGAG 2 cut(s) 218, 524
BsaJI CCNNGG 1 cut(s) 245
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse1I ACTGG 3 cut(s) 417, 446, 528
Bse3DI GCAATG 1 cut(s) 277
BseDI CCNNGG 1 cut(s) 245
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 2 cut(s) 277, 330
BseNI ACTGG 3 cut(s) 417, 446, 528
BseSI GKGCMC 1 cut(s) 535
BseXI GCAGC 2 cut(s) 423, 530
BsgI GTGCAG 1 cut(s) 336
BsiHKAI GWGCWC 2 cut(s) 407, 535
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 1 cut(s) 61
BsmFI GGGAC 1 cut(s) 136
BsmI GAATGC 1 cut(s) 475
Bsp1286I GDGCHC 2 cut(s) 407, 535
Bsp143I GATC 4 cut(s) 216, 229, 342, 361
BspACI CCGC 2 cut(s) 107, 545
BspCNI CTCAG 2 cut(s) 276, 331
BspMAI CTGCAG 1 cut(s) 97
BspQI GCTCTTC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 277
BsrI ACTGG 3 cut(s) 417, 446, 528
BssECI CCNNGG 1 cut(s) 245
BssMI GATC 4 cut(s) 216, 229, 342, 361
BssT1I CCWWGG 1 cut(s) 245
Bst4CI ACNGT 1 cut(s) 322
Bst6I CTCTTC 3 cut(s) 48, 62, 206
BstC8I GCNNGC 2 cut(s) 135, 290
BstDEI CTNAG 2 cut(s) 263, 339
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 2 cut(s) 170, 385
BstKTI GATC 4 cut(s) 219, 232, 345, 364
BstMBI GATC 4 cut(s) 216, 229, 342, 361
BstMWI GCNNNNNNNGC 2 cut(s) 165, 411
BstNSI RCATGY 1 cut(s) 490
BstSFI CTRYAG 2 cut(s) 93, 163
BstSLI GKGCMC 1 cut(s) 535
BstV1I GCAGC 2 cut(s) 423, 530
BstX2I RGATCY 1 cut(s) 342
BstXI CCANNNNNNTGG 1 cut(s) 393
BstYI RGATCY 1 cut(s) 342
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 5 cut(s) 318, 353, 424, 486, 535
Cac8I GCNNGC 2 cut(s) 135, 290
CfoI GCGC 2 cut(s) 170, 385
Cfr13I GGNCC 1 cut(s) 296
CviAII CATG 4 cut(s) 293, 428, 487, 491
DdeI CTNAG 2 cut(s) 263, 339
DpnI GATC 4 cut(s) 218, 231, 344, 363
DpnII GATC 4 cut(s) 216, 229, 342, 361
Eam1104I CTCTTC 3 cut(s) 48, 62, 206
EarI CTCTTC 3 cut(s) 48, 62, 206
EciI GGCGGA 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 245
Eco47I GGWCC 1 cut(s) 296
EcoT14I CCWWGG 1 cut(s) 245
EcoT22I ATGCAT 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 245
FaeI CATG 4 cut(s) 296, 431, 490, 494
FaiI YATR 7 cut(s) 294, 401, 429, 488, 492, 516, 555
FaqI GGGAC 1 cut(s) 136
FatI CATG 4 cut(s) 292, 427, 486, 490
Fnu4HI GCNGC 2 cut(s) 412, 519
Fsp4HI GCNGC 2 cut(s) 412, 519
GlaI GCGC 2 cut(s) 169, 384
GluI GCNGC 2 cut(s) 412, 519
GsuI CTGGAG 1 cut(s) 521
HaeII RGCGCY 1 cut(s) 171
HhaI GCGC 2 cut(s) 170, 385
Hin1II CATG 4 cut(s) 296, 431, 490, 494
Hin6I GCGC 2 cut(s) 168, 383
HinP1I GCGC 2 cut(s) 168, 383
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 4 cut(s) 116, 149, 424, 451
Hpy166II GTNNAC 3 cut(s) 75, 484, 533
Hpy188I TCNGA 3 cut(s) 115, 266, 304
Hpy8I GTNNAC 3 cut(s) 75, 484, 533
HpyAV CCTTC 2 cut(s) 93, 120
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4V TGCA 6 cut(s) 95, 335, 353, 490, 518, 533
HpyF10VI GCNNNNNNNGC 2 cut(s) 165, 411
HpyF3I CTNAG 2 cut(s) 263, 339
Hsp92II CATG 4 cut(s) 296, 431, 490, 494
HspAI GCGC 2 cut(s) 168, 383
Kzo9I GATC 4 cut(s) 216, 229, 342, 361
LguI GCTCTTC 1 cut(s) 206
LpnPI CCDG 9 cut(s) 105, 119, 302, 331, 343, 427, 430, 541, 551
Lsp1109I GCAGC 2 cut(s) 423, 530
LweI GCATC 1 cut(s) 556
MaeIII GTNAC 1 cut(s) 430
MalI GATC 4 cut(s) 218, 231, 344, 363
MboI GATC 4 cut(s) 216, 229, 342, 361
MboII GAAGA 4 cut(s) 35, 49, 81, 193
MflI RGATCY 1 cut(s) 342
MhlI GDGCHC 2 cut(s) 407, 535
MluCI AATT 1 cut(s) 184
MlyI GAGTC 2 cut(s) 158, 433
MnlI CCTC 3 cut(s) 19, 65, 552
Mph1103I ATGCAT 1 cut(s) 492
MroXI GAANNNNTTC 1 cut(s) 31
MslI CAYNNNNRTG 2 cut(s) 304, 552
MspA1I CMGCKG 1 cut(s) 521
Mva1269I GAATGC 1 cut(s) 475
MwoI GCNNNNNNNGC 2 cut(s) 165, 411
NdeII GATC 4 cut(s) 216, 229, 342, 361
NlaIII CATG 4 cut(s) 296, 431, 490, 494
NsiI ATGCAT 1 cut(s) 492
NspI RCATGY 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 206
PctI GAATGC 1 cut(s) 475
PdmI GAANNNNTTC 1 cut(s) 31
PfeI GAWTC 2 cut(s) 116, 451
PkrI GCNGC 2 cut(s) 413, 520
PleI GAGTC 2 cut(s) 157, 432
PpsI GAGTC 2 cut(s) 157, 432
PspPI GGNCC 1 cut(s) 296
PstI CTGCAG 1 cut(s) 97
PsuI RGATCY 1 cut(s) 342
PvuII CAGCTG 1 cut(s) 521
RseI CAYNNNNRTG 2 cut(s) 304, 552
SapI GCTCTTC 1 cut(s) 206
SatI GCNGC 2 cut(s) 412, 519
Sau3AI GATC 4 cut(s) 216, 229, 342, 361
Sau96I GGNCC 1 cut(s) 296
SchI GAGTC 2 cut(s) 158, 433
SduI GDGCHC 2 cut(s) 407, 535
SetI ASST 8 cut(s) 192, 201, 216, 240, 264, 504, 510, 523
SfaNI GCATC 1 cut(s) 556
SfcI CTRYAG 2 cut(s) 93, 163
SinI GGWCC 1 cut(s) 296
SmiMI CAYNNNNRTG 2 cut(s) 304, 552
SmlI CTYRAG 2 cut(s) 233, 503
SmoI CTYRAG 2 cut(s) 233, 503
Sse9I AATT 1 cut(s) 184
SsiI CCGC 2 cut(s) 107, 545
StyI CCWWGG 1 cut(s) 245
TaaI ACNGT 1 cut(s) 322
TaqI TCGA 2 cut(s) 18, 228
TasI AATT 1 cut(s) 184
TfiI GAWTC 2 cut(s) 116, 451
TscAI CASTG 5 cut(s) 325, 360, 424, 486, 535
TseI GCWGC 2 cut(s) 411, 518
TspDTI ATGAA 2 cut(s) 75, 166
TspRI CASTG 5 cut(s) 325, 360, 424, 486, 535
VneI GTGCAC 1 cut(s) 531
VpaK11BI GGWCC 1 cut(s) 296
XceI RCATGY 1 cut(s) 490
XmnI GAANNNNTTC 1 cut(s) 31
Zsp2I ATGCAT 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.