pycom03g05170

Zinc transporter 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0014136
Physical Location & Seq
Forward (+)
4005972 .. 4007530
1559 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g05170.1

Sequence Viewer

Length: 846 bp
ATGGCGTCTTGCGTATCGGACACGGCTCGGTCCCTCTCCTGCCGAGACGGACGCGCCGCCGCGCACCTCAAGCTAATCTCAATCGCCGCCATATTCGTCACCAGCGTCGTCGGCATATCGTCCCCCGTCCTCCTCTCCCGCTACTTCCAAGGCAAGCCCGCGTACGACAAAGCCACGCTCGTCATCAAGTGCTTCGCCGCCGGCGTCATCCTCTCCACCTCCCTCGTCCACGTCCTCCCCGACGCCTTCCACGCCCTCTCCGATTGCCGCGTGTCATCCCACCACCCCTGGAAGGACTACCCCTTCTCGGGGCTCGTCACCATGATCGGCGCTTTAATCGCCCTCCTCGTCGACCTCACCGCCACCTCCCACATGGAGTCCCACTCCGCCGTTGGCGGCCACGGTCACGGCGGATACGAGCTGGTGCCTGTGGGGGCGCGGGAGGAATTGGTGGGGAAGAATAGCAATTTGAAGGGTAAGAATAGCAAGCTGTCGGTGGATGTGGAGGTGGCGGTGGGTGTGGAGAGCAACAGTGGTGGGGGTAGTGAGGAAATGTTGATCCGGATGAAGCAGCGGCTGGTTTCGCAGGTGTTGGAAATCGGGATTATTTTCCACTCAGTGATAATTGGGGTGACGATGGGGATGTCGCAGAACCAGTGCACCATCCGGCCGTTGGTTGCTGCGCTGTCGTTTCACCAGATTTTCGAAGGGATGGGACTCGGAGGATGCATTGCTCAGGCAGGATTTAAGTTTGGGACAACAGCCTACATGTGCTTCATGTTTGCAGTGACAACTCCAATGGGGATAGTATTGGGGATCGATCTTATTCTCTGCGACAGGTTATGA

Protein Analysis

282

Amino Acids

29.73

Weight (kDa)

7.65

Isoelectric Point (pI)

25.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 22 - 273 1.1e-55 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30080
fragaria_vesca FvH4_3g39100
malus_domestica MD03G1064200.v1.1 MD11G1068000.v1.1
prunus_persica Prupe.6G053500_v2.0.a1
pyrus_communis pycom03g05170
rosa_chinensis RchiOBHm_Chr5g0070461
rosa_laevigata RLG00000036134
rosa_multiflora Rmu_co8511571.1_g000001 Rmu_sc0007961.1_g000008
rosa_roxburghii Rroxscaffold_1G00010750
rosa_rugosa Rorug05G0406100
rosa_samantha Rh5AG461300 Rh5BG479600 Rh5CG503800 Rh5DG491700
rosa_wichuraiana Rw5G043000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 577
Acc36I ACCTGC 1 cut(s) 577
AccB1I GGYRCC 1 cut(s) 424
AccI GTMKAC 1 cut(s) 351
AccII CGCG 5 cut(s) 54, 62, 161, 270, 439
AccIII TCCGGA 1 cut(s) 561
AclWI GGATC 2 cut(s) 553, 824
AcoI YGGCCR 2 cut(s) 397, 668
AcyI GRCGYC 3 cut(s) 5, 204, 243
AdeI CACNNNGTG 1 cut(s) 619
AfaI GTAC 1 cut(s) 164
AfiI CCNNNNNNNGG 5 cut(s) 292, 307, 308, 309, 673
AflIII ACRYGT 1 cut(s) 768
AgsI TTSAA 1 cut(s) 472
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 1 cut(s) 287
AluBI AGCT 3 cut(s) 73, 421, 490
AluI AGCT 3 cut(s) 73, 421, 490
Alw21I GWGCWC 1 cut(s) 662
Alw26I GTCTC 1 cut(s) 39
Alw44I GTGCAC 1 cut(s) 658
AlwI GGATC 2 cut(s) 553, 824
AlwNI CAGNNNCTG 1 cut(s) 577
Ama87I CYCGRG 1 cut(s) 307
Aor13HI TCCGGA 1 cut(s) 561
AoxI GGCC 2 cut(s) 397, 668
ApaLI GTGCAC 1 cut(s) 658
ApeKI GCWGC 2 cut(s) 571, 680
AspLEI GCGC 5 cut(s) 56, 64, 332, 439, 685
AspS9I GGNCC 1 cut(s) 30
AsuHPI GGTGA 5 cut(s) 91, 310, 349, 643, 686
AsuII TTCGAA 1 cut(s) 705
AvaI CYCGRG 1 cut(s) 307
AvaII GGWCC 1 cut(s) 30
BaeGI GKGCMC 1 cut(s) 662
BanI GGYRCC 1 cut(s) 424
BanII GRGCYC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 662
BbvI GCAGC 2 cut(s) 583, 667
BccI CCATC 3 cut(s) 631, 671, 706
BceAI ACGGC 4 cut(s) 39, 374, 424, 655
BciT130I CCWGG 1 cut(s) 289
BciVI GTATCC 1 cut(s) 407
BcoDI GTCTC 1 cut(s) 39
BfoI RGCGCY 1 cut(s) 333
BfuAI ACCTGC 1 cut(s) 577
BfuI GTATCC 1 cut(s) 407
BisI GCNGC 9 cut(s) 57, 60, 87, 198, 268, 397, 572, 575, 681
BlsI GCNGC 9 cut(s) 58, 61, 88, 199, 269, 398, 573, 576, 682
Bme1390I CCNGG 1 cut(s) 289
Bme18I GGWCC 1 cut(s) 30
BmeT110I CYCGRG 1 cut(s) 307
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 30
BmiI GGNNCC 2 cut(s) 32, 426
BmrFI CCNGG 1 cut(s) 289
BmsI GCATC 1 cut(s) 716
BplI GAGNNNNNCTC 2 cut(s) 368, 400
Bpu10I CCTNAGC 1 cut(s) 735
Bpu14I TTCGAA 1 cut(s) 705
BpuEI CTTGAG 1 cut(s) 53
Bsa29I ATCGAT 1 cut(s) 819
BsaHI GRCGYC 3 cut(s) 5, 204, 243
BsaJI CCNNGG 3 cut(s) 148, 287, 400
BsaWI WCCGGW 1 cut(s) 561
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 5 cut(s) 292, 307, 308, 309, 673
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 655
Bse3DI GCAATG 1 cut(s) 729
BseAI TCCGGA 1 cut(s) 561
BseBI CCWGG 1 cut(s) 289
BseCI ATCGAT 1 cut(s) 819
BseDI CCNNGG 3 cut(s) 148, 287, 400
BseGI GGATG 8 cut(s) 207, 275, 505, 570, 648, 663, 717, 731
BseLI CCNNNNNNNGG 5 cut(s) 292, 307, 308, 309, 673
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 2 cut(s) 630, 749
BseNI ACTGG 1 cut(s) 655
BseRI GAGGAG 2 cut(s) 122, 335
BseSI GKGCMC 1 cut(s) 662
BseX3I CGGCCG 1 cut(s) 668
BseXI GCAGC 2 cut(s) 583, 667
Bsh1236I CGCG 5 cut(s) 54, 62, 161, 270, 439
Bsh1285I CGRYCG 1 cut(s) 671
BshFI GGCC 2 cut(s) 399, 670
BshNI GGYRCC 1 cut(s) 424
BshVI ATCGAT 1 cut(s) 819
BsiEI CGRYCG 1 cut(s) 671
BsiHKAI GWGCWC 1 cut(s) 662
BsiHKCI CYCGRG 1 cut(s) 307
BsiSI CCGG 3 cut(s) 201, 562, 667
BsiWI CGTACG 1 cut(s) 162
BslFI GGGAC 5 cut(s) 16, 106, 364, 729, 769
BslI CCNNNNNNNGG 5 cut(s) 292, 307, 308, 309, 673
BsmAI GTCTC 1 cut(s) 39
BsmBI CGTCTC 1 cut(s) 39
BsmFI GGGAC 5 cut(s) 16, 106, 364, 729, 769
BsnI GGCC 2 cut(s) 399, 670
BsoBI CYCGRG 1 cut(s) 307
Bsp119I TTCGAA 1 cut(s) 705
Bsp1286I GDGCHC 2 cut(s) 315, 662
Bsp13I TCCGGA 1 cut(s) 561
Bsp143I GATC 4 cut(s) 324, 558, 816, 820
BspANI GGCC 2 cut(s) 399, 670
BspCNI CTCAG 2 cut(s) 629, 748
BspDI ATCGAT 1 cut(s) 819
BspEI TCCGGA 1 cut(s) 561
BspFNI CGCG 5 cut(s) 54, 62, 161, 270, 439
BspLI GGNNCC 2 cut(s) 32, 426
BspMI ACCTGC 1 cut(s) 577
BspPI GGATC 2 cut(s) 553, 824
BspT104I TTCGAA 1 cut(s) 705
BspT107I GGYRCC 1 cut(s) 424
BsrDI GCAATG 1 cut(s) 729
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 1 cut(s) 655
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 3 cut(s) 148, 287, 400
BssMI GATC 4 cut(s) 324, 558, 816, 820
BssNI GRCGYC 3 cut(s) 5, 204, 243
BssT1I CCWWGG 1 cut(s) 148
Bst2UI CCWGG 1 cut(s) 289
Bst4CI ACNGT 2 cut(s) 404, 533
BstACI GRCGYC 3 cut(s) 5, 204, 243
BstBI TTCGAA 1 cut(s) 705
BstC8I GCNNGC 4 cut(s) 155, 159, 202, 488
BstDEI CTNAG 2 cut(s) 616, 735
BstDSI CCRYGG 1 cut(s) 400
BstF5I GGATG 8 cut(s) 207, 275, 505, 570, 648, 663, 717, 731
BstFNI CGCG 5 cut(s) 54, 62, 161, 270, 439
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 5 cut(s) 56, 64, 332, 439, 685
BstKTI GATC 4 cut(s) 327, 561, 819, 823
BstMAI GTCTC 1 cut(s) 39
BstMBI GATC 4 cut(s) 324, 558, 816, 820
BstMCI CGRYCG 1 cut(s) 671
BstMWI GCNNNNNNNGC 5 cut(s) 70, 111, 251, 338, 583
BstNI CCWGG 1 cut(s) 289
BstNSI RCATGY 1 cut(s) 772
BstSCI CCNGG 1 cut(s) 287
BstSLI GKGCMC 1 cut(s) 662
BstUI CGCG 5 cut(s) 54, 62, 161, 270, 439
BstV1I GCAGC 2 cut(s) 583, 667
BstZI CGGCCG 1 cut(s) 668
Bsu15I ATCGAT 1 cut(s) 819
BsuI GTATCC 1 cut(s) 407
BsuRI GGCC 2 cut(s) 399, 670
BsuTUI ATCGAT 1 cut(s) 819
BtgI CCRYGG 1 cut(s) 400
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 8 cut(s) 207, 275, 505, 570, 648, 663, 717, 731
BtsI GCAGTG 1 cut(s) 792
BtsIMutI CAGTG 4 cut(s) 538, 624, 662, 792
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 4 cut(s) 155, 159, 202, 488
CaiI CAGNNNCTG 1 cut(s) 577
CfoI GCGC 5 cut(s) 56, 64, 332, 439, 685
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 1 cut(s) 30
ClaI ATCGAT 1 cut(s) 819
CseI GACGC 4 cut(s) 60, 94, 193, 251
Csp6I GTAC 1 cut(s) 163
CspCI CAANNNNNGTGG 2 cut(s) 517, 552
CviAII CATG 4 cut(s) 322, 373, 769, 778
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 2 cut(s) 616, 735
DpnI GATC 4 cut(s) 326, 560, 818, 822
DpnII GATC 4 cut(s) 324, 558, 816, 820
DraIII CACNNNGTG 1 cut(s) 619
EaeI YGGCCR 2 cut(s) 397, 668
EagI CGGCCG 1 cut(s) 668
EciI GGCGGA 2 cut(s) 376, 426
EclXI CGGCCG 1 cut(s) 668
Eco130I CCWWGG 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 315
Eco47I GGWCC 1 cut(s) 30
Eco52I CGGCCG 1 cut(s) 668
Eco88I CYCGRG 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 148
EcoT22I ATGCAT 1 cut(s) 731
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 148
Esp3I CGTCTC 1 cut(s) 39
FaeI CATG 4 cut(s) 325, 376, 772, 781
FaiI YATR 7 cut(s) 92, 116, 323, 374, 770, 779, 844
FaqI GGGAC 5 cut(s) 16, 106, 364, 729, 769
FatI CATG 4 cut(s) 321, 372, 768, 777
FauI CCCGC 3 cut(s) 146, 166, 432
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 9 cut(s) 57, 60, 87, 198, 268, 397, 572, 575, 681
FokI GGATG 8 cut(s) 194, 262, 512, 577, 650, 655, 724, 738
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 9 cut(s) 57, 60, 87, 198, 268, 397, 572, 575, 681
GlaI GCGC 5 cut(s) 55, 63, 331, 438, 684
GluI GCNGC 9 cut(s) 57, 60, 87, 198, 268, 397, 572, 575, 681
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 2 cut(s) 399, 670
HapII CCGG 3 cut(s) 201, 562, 667
HgaI GACGC 4 cut(s) 60, 94, 193, 251
HhaI GCGC 5 cut(s) 56, 64, 332, 439, 685
Hin1I GRCGYC 3 cut(s) 5, 204, 243
Hin1II CATG 4 cut(s) 325, 376, 772, 781
Hin6I GCGC 5 cut(s) 54, 62, 330, 437, 683
HinP1I GCGC 5 cut(s) 54, 62, 330, 437, 683
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 2 cut(s) 377, 717
HpaII CCGG 3 cut(s) 201, 562, 667
HphI GGTGA 5 cut(s) 91, 310, 349, 643, 686
Hpy166II GTNNAC 3 cut(s) 229, 352, 660
Hpy188I TCNGA 3 cut(s) 19, 262, 722
Hpy188III TCNNGA 2 cut(s) 562, 601
Hpy8I GTNNAC 3 cut(s) 229, 352, 660
Hpy99I CGWCG 4 cut(s) 110, 113, 245, 353
HpyAV CCTTC 5 cut(s) 256, 286, 313, 466, 701
HpyCH4III ACNGT 2 cut(s) 404, 533
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 3 cut(s) 660, 729, 785
HpyF10VI GCNNNNNNNGC 5 cut(s) 70, 111, 251, 338, 583
HpyF3I CTNAG 2 cut(s) 616, 735
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 3 cut(s) 5, 204, 243
Hsp92II CATG 4 cut(s) 325, 376, 772, 781
HspAI GCGC 5 cut(s) 54, 62, 330, 437, 683
Kpn2I TCCGGA 1 cut(s) 561
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Kzo9I GATC 4 cut(s) 324, 558, 816, 820
Lsp1109I GCAGC 2 cut(s) 583, 667
LweI GCATC 1 cut(s) 716
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 5 cut(s) 97, 316, 404, 631, 787
MalI GATC 4 cut(s) 326, 560, 818, 822
MboI GATC 4 cut(s) 324, 558, 816, 820
MboII GAAGA 1 cut(s) 469
MhlI GDGCHC 2 cut(s) 315, 662
MluCI AATT 3 cut(s) 446, 466, 624
MlyI GAGTC 2 cut(s) 386, 711
MmeI TCCRAC 1 cut(s) 573
Mph1103I ATGCAT 1 cut(s) 731
MreI CGCCGGCG 1 cut(s) 200
MroI TCCGGA 1 cut(s) 561
MroNI GCCGGC 1 cut(s) 200
MseI TTAA 2 cut(s) 335, 747
MspA1I CMGCKG 1 cut(s) 574
MspI CCGG 3 cut(s) 201, 562, 667
MspR9I CCNGG 1 cut(s) 289
MvaI CCWGG 1 cut(s) 289
MvnI CGCG 5 cut(s) 54, 62, 161, 270, 439
MwoI GCNNNNNNNGC 5 cut(s) 70, 111, 251, 338, 583
NaeI GCCGGC 1 cut(s) 202
NdeII GATC 4 cut(s) 324, 558, 816, 820
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 4 cut(s) 325, 376, 772, 781
NlaIV GGNNCC 2 cut(s) 32, 426
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 5 cut(s) 97, 316, 404, 631, 787
NsiI ATGCAT 1 cut(s) 731
NspI RCATGY 1 cut(s) 772
NspV TTCGAA 1 cut(s) 705
PaqCI CACCTGC 1 cut(s) 577
PciI ACATGT 1 cut(s) 768
PcsI WCGNNNNNNNCGW 6 cut(s) 102, 201, 237, 258, 345, 414
PdiI GCCGGC 1 cut(s) 202
Pfl23II CGTACG 1 cut(s) 162
PkrI GCNGC 9 cut(s) 58, 61, 88, 199, 269, 398, 573, 576, 682
PleI GAGTC 2 cut(s) 385, 711
PpsI GAGTC 2 cut(s) 385, 711
PscI ACATGT 1 cut(s) 768
Psp6I CCWGG 1 cut(s) 287
PspGI CCWGG 1 cut(s) 287
PspLI CGTACG 1 cut(s) 162
PspN4I GGNNCC 2 cut(s) 32, 426
PspPI GGNCC 1 cut(s) 30
PstNI CAGNNNCTG 1 cut(s) 577
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
SalI GTCGAC 1 cut(s) 350
SaqAI TTAA 2 cut(s) 335, 747
SatI GCNGC 9 cut(s) 57, 60, 87, 198, 268, 397, 572, 575, 681
Sau3AI GATC 4 cut(s) 324, 558, 816, 820
Sau96I GGNCC 1 cut(s) 30
SchI GAGTC 2 cut(s) 386, 711
ScrFI CCNGG 1 cut(s) 289
SduI GDGCHC 2 cut(s) 315, 662
SfaNI GCATC 1 cut(s) 716
SfuI TTCGAA 1 cut(s) 705
SgrAI CRCCGGYG 1 cut(s) 200
SinI GGWCC 1 cut(s) 30
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 3 cut(s) 446, 466, 624
StyD4I CCNGG 1 cut(s) 287
StyI CCWWGG 1 cut(s) 148
TaaI ACNGT 2 cut(s) 404, 533
TaiI ACGT 1 cut(s) 234
TaqI TCGA 3 cut(s) 351, 705, 819
TaqII GACCGA 1 cut(s) 18
TasI AATT 3 cut(s) 446, 466, 624
TauI GCSGC 7 cut(s) 59, 62, 89, 200, 270, 399, 577
Tru1I TTAA 2 cut(s) 335, 747
Tru9I TTAA 2 cut(s) 335, 747
TscAI CASTG 4 cut(s) 538, 624, 662, 792
TseFI GTSAC 5 cut(s) 97, 316, 404, 631, 787
TseI GCWGC 2 cut(s) 571, 680
Tsp45I GTSAC 5 cut(s) 97, 316, 404, 631, 787
TspDTI ATGAA 2 cut(s) 581, 766
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 4 cut(s) 538, 624, 662, 792
VneI GTGCAC 1 cut(s) 658
VpaK11BI GGWCC 1 cut(s) 30
XceI RCATGY 1 cut(s) 772
XcmI CCANNNNNNNNNTGG 3 cut(s) 370, 389, 670
XmiI GTMKAC 1 cut(s) 351
Zsp2I ATGCAT 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.