Rw5G043000

Zinc transporter 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014136
Physical Location & Seq
Reverse (-)
75167824 .. 75170277
2454 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G043000.1

Sequence Viewer

Length: 1038 bp
ATGGCCGCGTGCGGGGCCGACACGGCCCGAACCATCGCTTGCCGCGACGGCCACGCGGCCGCGCAGCTGAAGCTCATCTCCATCGCCGTCATCTTCATCACCAGCGTCGTCGGCATCACCTCCCCTGTCCTCCTCGCCCGTCACTTCCAAGGCAAGCCCGCCTACGACCGCGCTACTCTCATAATCAAGTGCTTCGCCGCCGGCGTCATCCTCTCCACTTCCCTCGTCCACGTCCTCCCCGATGCCTTCGCCGCCCTAGCCGACTGCCACGTGGCCTCCCGCCATCCCTGGAAGGACTACCCCTTCTCCGGCCTCATCACCATGATCGGCGCCATCGCCGCCCTCCTCGTCGACCTCACCGCCAGCGCCCACATGGAGAGCCACAGCACCCACGCCCACGGCCAATACGAGGTCCTTTCGGTGAGCACACAGGAGGAGCTAGGGACCAAGAAGTCGTCGCCGATCGAGTTGAAAGTTGAGTCCGTGGAGGGTGCAGTTAGCGATAGTGGCGGTGGTGATAGCCATAGCCATGAGGAGATGATCATTCGGCTGAAGCAGAGATTGGTGTCGCAGGTGTTGGAGATAGGGATAATATTTCACTCGGTGATTATAGGAGTGACGATGGGGATGTCTCAGAACCAGTGCACCATTCGGCCATTGGTGGCTGCGCTTGCGTTTCATCAGATTTTCGAGGGGATGGGCCTCGGCGGCTGCATTGCTCAGGCAGGGTTTACCTTTGGAACAACGGCCTATATGTGCTTCATGTTTGCAGTGACGACCCCAATGGGGATAGTGTTGGGGATGATCTTATTCTCGGTGACAGGTTACGACGATAGTAGCACTAATGCCTTAATCTTGGAGGGGTTGTTGGGGTCTTTCTCTTCAGGTATCCTCATTTACATGGCTCTTGTGGATCTCATAGCTCTTGATTTCTTCCACAACAAGATGATGAGCTCTAATTCATGGTTGAGGAAAACTTCATTCACTGCTCTTGTTCTTGGCTCCATTTCCATGTCCATCCTTGCCCTCTGGGCCTAG

Protein Analysis

345

Amino Acids

36.69

Weight (kDa)

6.13

Isoelectric Point (pI)

37.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 22 - 342 3.7e-72 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30080
fragaria_vesca FvH4_3g39100
malus_domestica MD03G1064200.v1.1 MD11G1068000.v1.1
prunus_persica Prupe.6G053500_v2.0.a1
pyrus_communis pycom03g05170
rosa_chinensis RchiOBHm_Chr5g0070461
rosa_laevigata RLG00000036134
rosa_multiflora Rmu_co8511571.1_g000001 Rmu_sc0007961.1_g000008
rosa_roxburghii Rroxscaffold_1G00010750
rosa_rugosa Rorug05G0406100
rosa_samantha Rh5AG461300 Rh5BG479600 Rh5CG503800 Rh5DG491700
rosa_wichuraiana Rw5G043000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 562
AasI GACNNNNNNGTC 1 cut(s) 451
Acc36I ACCTGC 1 cut(s) 562
AccB1I GGYRCC 1 cut(s) 329
AccI GTMKAC 1 cut(s) 351
AccII CGCG 5 cut(s) 8, 45, 56, 62, 171
AclWI GGATC 1 cut(s) 921
AcoI YGGCCR 5 cut(s) 3, 49, 57, 400, 653
AcuI CTGAAG 3 cut(s) 89, 572, 867
AcvI CACGTG 1 cut(s) 271
AcyI GRCGYC 2 cut(s) 204, 330
AdeI CACNNNGTG 1 cut(s) 604
AfiI CCNNNNNNNGG 4 cut(s) 12, 308, 409, 786
AgsI TTSAA 1 cut(s) 472
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 1 cut(s) 287
AjuI GAANNNNNNNTTGG 2 cut(s) 545, 577
AluBI AGCT 5 cut(s) 67, 73, 439, 923, 954
AluI AGCT 5 cut(s) 67, 73, 439, 923, 954
Alw21I GWGCWC 3 cut(s) 428, 647, 956
Alw26I GTCTC 1 cut(s) 636
Alw44I GTGCAC 1 cut(s) 643
AlwI GGATC 1 cut(s) 921
ApaLI GTGCAC 1 cut(s) 643
ApeKI GCWGC 3 cut(s) 64, 665, 711
AspLEI GCGC 5 cut(s) 64, 173, 332, 368, 670
AspS9I GGNCC 6 cut(s) 15, 25, 412, 444, 700, 1032
AsuHPI GGTGA 8 cut(s) 91, 109, 310, 349, 433, 527, 616, 829
AvaII GGWCC 2 cut(s) 412, 444
BaeGI GKGCMC 1 cut(s) 647
BanI GGYRCC 1 cut(s) 329
BanII GRGCYC 1 cut(s) 956
BbrPI CACGTG 1 cut(s) 271
Bbv12I GWGCWC 3 cut(s) 428, 647, 956
BbvI GCAGC 3 cut(s) 76, 652, 698
BccI CCATC 7 cut(s) 41, 89, 291, 341, 616, 691, 1025
BceAI ACGGC 5 cut(s) 39, 64, 71, 415, 762
BciT130I CCWGG 1 cut(s) 289
BciVI GTATCC 1 cut(s) 899
BclI TGATCA 1 cut(s) 540
BcoDI GTCTC 1 cut(s) 636
BfaI CTAG 3 cut(s) 257, 440, 1036
BfoI RGCGCY 2 cut(s) 333, 369
BfuAI ACCTGC 1 cut(s) 562
BfuI GTATCC 1 cut(s) 899
BglI GCCNNNNNGGC 4 cut(s) 23, 48, 708, 1031
Bme1390I CCNGG 1 cut(s) 289
Bme18I GGWCC 2 cut(s) 412, 444
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 6 cut(s) 15, 25, 412, 444, 700, 1032
BmiI GGNNCC 4 cut(s) 16, 331, 445, 1003
BmrFI CCNGG 1 cut(s) 289
BmsI GCATC 2 cut(s) 123, 232
Bpu10I CCTNAGC 1 cut(s) 720
BsaAI YACGTR 1 cut(s) 271
BsaHI GRCGYC 2 cut(s) 204, 330
BsaJI CCNNGG 5 cut(s) 148, 287, 397, 483, 703
BsaXI ACNNNNNCTCC 6 cut(s) 260, 290, 290, 320, 851, 881
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 308, 409, 786
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 640
Bse3DI GCAATG 1 cut(s) 714
BseBI CCWGG 1 cut(s) 289
BseDI CCNNGG 5 cut(s) 148, 287, 397, 483, 703
BseGI GGATG 6 cut(s) 207, 283, 633, 702, 807, 1017
BseLI CCNNNNNNNGG 4 cut(s) 12, 308, 409, 786
BseMI GCAATG 1 cut(s) 714
BseMII CTCAG 2 cut(s) 647, 734
BseNI ACTGG 1 cut(s) 640
BseRI GAGGAG 4 cut(s) 122, 335, 449, 548
BseSI GKGCMC 1 cut(s) 647
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 3 cut(s) 76, 652, 698
BsgI GTGCAG 1 cut(s) 513
Bsh1236I CGCG 5 cut(s) 8, 45, 56, 62, 171
Bsh1285I CGRYCG 3 cut(s) 60, 169, 465
BshNI GGYRCC 1 cut(s) 329
BsiEI CGRYCG 3 cut(s) 60, 169, 465
BsiHKAI GWGCWC 3 cut(s) 428, 647, 956
BsiSI CCGG 2 cut(s) 201, 309
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 4 cut(s) 12, 308, 409, 786
BsmAI GTCTC 1 cut(s) 636
BsmFI GGGAC 1 cut(s) 457
Bsp1286I GDGCHC 3 cut(s) 428, 647, 956
Bsp143I GATC 5 cut(s) 324, 462, 540, 804, 913
BspCNI CTCAG 2 cut(s) 646, 733
BspFNI CGCG 5 cut(s) 8, 45, 56, 62, 171
BspLI GGNNCC 4 cut(s) 16, 331, 445, 1003
BspMI ACCTGC 1 cut(s) 562
BspPI GGATC 1 cut(s) 921
BspT107I GGYRCC 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 714
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 1 cut(s) 640
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 5 cut(s) 148, 287, 397, 483, 703
BssMI GATC 5 cut(s) 324, 462, 540, 804, 913
BssNI GRCGYC 2 cut(s) 204, 330
BssT1I CCWWGG 1 cut(s) 148
Bst2UI CCWGG 1 cut(s) 289
Bst6I CTCTTC 1 cut(s) 886
BstACI GRCGYC 2 cut(s) 204, 330
BstBAI YACGTR 1 cut(s) 271
BstC8I GCNNGC 7 cut(s) 10, 40, 155, 159, 202, 364, 672
BstDEI CTNAG 2 cut(s) 633, 720
BstDSI CCRYGG 2 cut(s) 397, 483
BstF5I GGATG 6 cut(s) 207, 283, 633, 702, 807, 1017
BstFNI CGCG 5 cut(s) 8, 45, 56, 62, 171
BstH2I RGCGCY 2 cut(s) 333, 369
BstHHI GCGC 5 cut(s) 64, 173, 332, 368, 670
BstKTI GATC 5 cut(s) 327, 465, 543, 807, 916
BstMAI GTCTC 1 cut(s) 636
BstMBI GATC 5 cut(s) 324, 462, 540, 804, 913
BstMCI CGRYCG 3 cut(s) 60, 169, 465
BstNI CCWGG 1 cut(s) 289
BstSCI CCNGG 1 cut(s) 287
BstSLI GKGCMC 1 cut(s) 647
BstUI CGCG 5 cut(s) 8, 45, 56, 62, 171
BstV1I GCAGC 3 cut(s) 76, 652, 698
BstX2I RGATCY 1 cut(s) 913
BstYI RGATCY 1 cut(s) 913
BstZI CGGCCG 1 cut(s) 57
BsuI GTATCC 1 cut(s) 899
BtgI CCRYGG 2 cut(s) 397, 483
BtgZI GCGATG 3 cut(s) 19, 67, 319
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 6 cut(s) 207, 283, 633, 702, 807, 1017
BtsI GCAGTG 2 cut(s) 777, 984
BtsIMutI CAGTG 3 cut(s) 647, 777, 984
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 7 cut(s) 10, 40, 155, 159, 202, 364, 672
CciNI GCGGCCGC 1 cut(s) 57
CfoI GCGC 5 cut(s) 64, 173, 332, 368, 670
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 6 cut(s) 15, 25, 412, 444, 700, 1032
CseI GACGC 2 cut(s) 94, 193
CviAII CATG 7 cut(s) 322, 373, 530, 763, 901, 963, 1012
DdeI CTNAG 2 cut(s) 633, 720
DinI GGCGCC 1 cut(s) 331
DpnI GATC 5 cut(s) 326, 464, 542, 806, 915
DpnII GATC 5 cut(s) 324, 462, 540, 804, 913
DraIII CACNNNGTG 1 cut(s) 604
DrdI GACNNNNNNGTC 1 cut(s) 451
DseDI GACNNNNNNGTC 1 cut(s) 451
EaeI YGGCCR 5 cut(s) 3, 49, 57, 400, 653
EagI CGGCCG 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 886
EarI CTCTTC 1 cut(s) 886
Ecl136II GAGCTC 1 cut(s) 954
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 956
Eco47I GGWCC 2 cut(s) 412, 444
Eco52I CGGCCG 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 954
Eco57I CTGAAG 3 cut(s) 89, 572, 867
Eco72I CACGTG 1 cut(s) 271
EcoICRI GAGCTC 1 cut(s) 954
EcoO109I RGGNCCY 1 cut(s) 412
EcoRII CCWGG 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 148
EcoT38I GRGCYC 1 cut(s) 956
EgeI GGCGCC 1 cut(s) 331
EheI GGCGCC 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 148
FaeI CATG 7 cut(s) 325, 376, 533, 766, 904, 966, 1015
FaqI GGGAC 1 cut(s) 457
FatI CATG 7 cut(s) 321, 372, 529, 762, 900, 962, 1011
FauI CCCGC 3 cut(s) 5, 166, 287
FbaI TGATCA 1 cut(s) 540
FblI GTMKAC 1 cut(s) 351
FokI GGATG 6 cut(s) 194, 270, 640, 709, 814, 1004
FriOI GRGCYC 1 cut(s) 956
FspBI CTAG 3 cut(s) 257, 440, 1036
GlaI GCGC 5 cut(s) 63, 172, 331, 367, 669
HaeII RGCGCY 2 cut(s) 333, 369
HapII CCGG 2 cut(s) 201, 309
HgaI GACGC 2 cut(s) 94, 193
HhaI GCGC 5 cut(s) 64, 173, 332, 368, 670
Hin1I GRCGYC 2 cut(s) 204, 330
Hin1II CATG 7 cut(s) 325, 376, 533, 766, 904, 966, 1015
Hin6I GCGC 5 cut(s) 62, 171, 330, 366, 668
HinP1I GCGC 5 cut(s) 62, 171, 330, 366, 668
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 1 cut(s) 479
HpaII CCGG 2 cut(s) 201, 309
HphI GGTGA 8 cut(s) 91, 109, 310, 349, 433, 527, 616, 829
Hpy166II GTNNAC 4 cut(s) 229, 352, 645, 732
Hpy188I TCNGA 2 cut(s) 636, 684
Hpy188III TCNNGA 1 cut(s) 926
Hpy8I GTNNAC 4 cut(s) 229, 352, 645, 732
Hpy99I CGWCG 6 cut(s) 50, 110, 113, 353, 460, 833
HpyAV CCTTC 3 cut(s) 256, 286, 313
HpyCH4IV ACGT 2 cut(s) 231, 270
HpyCH4V TGCA 4 cut(s) 494, 645, 714, 770
HpyF3I CTNAG 2 cut(s) 633, 720
HpySE526I ACGT 2 cut(s) 231, 270
Hsp92I GRCGYC 2 cut(s) 204, 330
Hsp92II CATG 7 cut(s) 325, 376, 533, 766, 904, 966, 1015
HspAI GCGC 5 cut(s) 62, 171, 330, 366, 668
KasI GGCGCC 1 cut(s) 329
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Ksp22I TGATCA 1 cut(s) 540
Kzo9I GATC 5 cut(s) 324, 462, 540, 804, 913
LmnI GCTCC 2 cut(s) 436, 1007
Lsp1109I GCAGC 3 cut(s) 76, 652, 698
LweI GCATC 2 cut(s) 123, 232
MaeI CTAG 3 cut(s) 257, 440, 1036
MaeII ACGT 2 cut(s) 231, 270
MaeIII GTNAC 5 cut(s) 140, 616, 772, 817, 824
MalI GATC 5 cut(s) 326, 464, 542, 806, 915
MboI GATC 5 cut(s) 324, 462, 540, 804, 913
MboII GAAGA 3 cut(s) 85, 873, 925
MflI RGATCY 1 cut(s) 913
MhlI GDGCHC 3 cut(s) 428, 647, 956
MluCI AATT 1 cut(s) 958
Mly113I GGCGCC 1 cut(s) 330
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 558
MreI CGCCGGCG 1 cut(s) 200
MroNI GCCGGC 1 cut(s) 200
MseI TTAA 1 cut(s) 851
MslI CAYNNNNRTG 4 cut(s) 320, 528, 899, 1010
MspA1I CMGCKG 1 cut(s) 67
MspI CCGG 2 cut(s) 201, 309
MspR9I CCNGG 1 cut(s) 289
MvaI CCWGG 1 cut(s) 289
MvnI CGCG 5 cut(s) 8, 45, 56, 62, 171
NaeI GCCGGC 1 cut(s) 202
NarI GGCGCC 1 cut(s) 330
NdeII GATC 5 cut(s) 324, 462, 540, 804, 913
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 7 cut(s) 325, 376, 533, 766, 904, 966, 1015
NlaIV GGNNCC 4 cut(s) 16, 331, 445, 1003
NmeAIII GCCGAG 1 cut(s) 684
NmuCI GTSAC 4 cut(s) 140, 616, 772, 817
NotI GCGGCCGC 1 cut(s) 57
PaqCI CACCTGC 1 cut(s) 562
PcsI WCGNNNNNNNCGW 4 cut(s) 42, 201, 237, 405
PdiI GCCGGC 1 cut(s) 202
Ple19I CGATCG 1 cut(s) 465
PleI GAGTC 1 cut(s) 487
PluTI GGCGCC 1 cut(s) 333
PmaCI CACGTG 1 cut(s) 271
PmlI CACGTG 1 cut(s) 271
PpsI GAGTC 1 cut(s) 487
Ppu21I YACGTR 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 412
Psp124BI GAGCTC 1 cut(s) 956
Psp5II RGGWCCY 1 cut(s) 412
Psp6I CCWGG 1 cut(s) 287
PspCI CACGTG 1 cut(s) 271
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 4 cut(s) 16, 331, 445, 1003
PspPI GGNCC 6 cut(s) 15, 25, 412, 444, 700, 1032
PspPPI RGGWCCY 1 cut(s) 412
PsuI RGATCY 1 cut(s) 913
PvuI CGATCG 1 cut(s) 465
PvuII CAGCTG 1 cut(s) 67
RseI CAYNNNNRTG 4 cut(s) 320, 528, 899, 1010
SacI GAGCTC 1 cut(s) 956
SalI GTCGAC 1 cut(s) 350
SaqAI TTAA 1 cut(s) 851
Sau3AI GATC 5 cut(s) 324, 462, 540, 804, 913
Sau96I GGNCC 6 cut(s) 15, 25, 412, 444, 700, 1032
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 1 cut(s) 289
SduI GDGCHC 3 cut(s) 428, 647, 956
SfaNI GCATC 2 cut(s) 123, 232
SfiI GGCCNNNNNGGCC 1 cut(s) 23
SfoI GGCGCC 1 cut(s) 331
SgrAI CRCCGGYG 1 cut(s) 200
SinI GGWCC 2 cut(s) 412, 444
SmiMI CAYNNNNRTG 4 cut(s) 320, 528, 899, 1010
Sse9I AATT 1 cut(s) 958
SspDI GGCGCC 1 cut(s) 329
SspI AATATT 1 cut(s) 594
SspMI CTAG 3 cut(s) 257, 440, 1036
SstI GAGCTC 1 cut(s) 956
StyD4I CCNGG 1 cut(s) 287
StyI CCWWGG 1 cut(s) 148
TaiI ACGT 2 cut(s) 234, 273
TaqI TCGA 3 cut(s) 351, 465, 690
TasI AATT 1 cut(s) 958
TauI GCSGC 8 cut(s) 8, 45, 59, 62, 200, 254, 341, 711
Tru1I TTAA 1 cut(s) 851
Tru9I TTAA 1 cut(s) 851
TscAI CASTG 3 cut(s) 647, 777, 991
TseFI GTSAC 4 cut(s) 140, 616, 772, 817
TseI GCWGC 3 cut(s) 64, 665, 711
Tsp45I GTSAC 4 cut(s) 140, 616, 772, 817
TspDTI ATGAA 5 cut(s) 85, 668, 751, 951, 969
TspGWI ACGGA 1 cut(s) 472
TspRI CASTG 3 cut(s) 647, 777, 991
VneI GTGCAC 1 cut(s) 643
VpaK11BI GGWCC 2 cut(s) 412, 444
XcmI CCANNNNNNNNNTGG 2 cut(s) 370, 655
XmiI GTMKAC 1 cut(s) 351
XspI CTAG 3 cut(s) 257, 440, 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.