pycom03g12040

Phosphoesterase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0011842
Physical Location & Seq
Forward (+)
12391488 .. 12393788
2301 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 924 bp
ATGGCCACCGCCGCCTCCACAACCACCATTTCAATCCTCCTTCTGCTGCTCATAGGACTCAACAGTCCAGTCCATGCTAATCCCATCAAAACCATAGTTGTTATAGTGATGGAGAACAGGTCATTCGACCACATGCTGGGGTGGATGAAGAAAATCAACCCTGAAATCAACGGCGTGGATGGCTCCGAGGCCAACCCTCTCTCCACCACCGACCCTAACTCCAAGCGCTTCTTCTTCAACAACGAGTCTCACTATGTGGACCCTGATCGATCTGGCCACTCCTTCCAAGCCATCCGAGAGCAGATATTCGGGTCGGATAACACGTCGGCCGACCCTCCACCCATGAATGGCTTTGCTCAGCAGGCATACTCCATGGACAACACCACCGCCCTGTCTCAGGATGTTATGAATGGATTCAACCCTGACATGGTTGCTGTCTACAAAACTCTTGTTTCTGAATTTGCTGTCTTTGACAGGTGGTTCGCCTCAGTGCCATCCTCAACACAGCCAAATCGGCTCTACGTACATTCAGGGACATCAGCTGGTGCAACAAGCAACATCCCAGCCCTTCTCGCCAAAGGGTACCCTCAAAGAACCATCTTCGAGAACCTCGACGACGCTGGAATATCCTTTGGAATATACTACCAAAACATCCCATCGACATTGTTCTATAGGAACCTGAGGAAGCTCAAGTACATAGGTAAGTTCCATTTGTACGACACCTTCAAAAGCCATGCAAAGGAAGGGAAACTGCCGGCGTACACCGTCATAGAACAGAGGTACTTCGATGTCAAGAGTGCACCAGCAAATGATGATCATCCCTCCCATGACGTGTACCACGGCCAGATGTTTGTGAAGGAGGTGTATGAGACACTGAGAGCAAGTCCTGGGTGGAACAACACCCTGATGATCGATCACGTATGA

Protein Analysis

308

Amino Acids

34.36

Weight (kDa)

6.11

Isoelectric Point (pI)

35.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phosphoesterase PF04185 29 - 304 6.6e-70 Phosphoesterase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011842)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26870
fragaria_vesca FvH4_3g27850
malus_domestica MD03G1165700.v1.1 MD11G1185000.v1.1
prunus_persica Prupe.4G266000_v2.0.a1
pyrus_communis pycom03g12040 pycom12g08790
rosa_chinensis RchiOBHm_Chr5g0050581
rosa_laevigata RLG00000034707 RLG00000034708 RLG00000034709
rosa_multiflora Rmu_sc0001954.1_g000003
rosa_roxburghii Rroxscaffold_1G00030270
rosa_rugosa Rorug05G0262100
rosa_samantha Rh5AG333700 Rh5BG343500 Rh5CG371100 Rh5DG356300
rosa_wichuraiana Rw5G031500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 63
Acc65I GGTACC 1 cut(s) 582
AccB1I GGYRCC 1 cut(s) 582
AccB7I CCANNNNNTGG 1 cut(s) 136
AccI GTMKAC 1 cut(s) 438
AciI CCGC 3 cut(s) 9, 12, 387
AcoI YGGCCR 4 cut(s) 3, 274, 327, 841
AcsI RAATTY 1 cut(s) 458
AdeI CACNNNGTG 1 cut(s) 256
AfaI GTAC 7 cut(s) 525, 584, 695, 716, 761, 782, 836
AfeI AGCGCT 1 cut(s) 227
AfiI CCNNNNNNNGG 5 cut(s) 136, 347, 397, 427, 739
AflIII ACRYGT 2 cut(s) 321, 831
AgsI TTSAA 4 cut(s) 33, 238, 418, 727
AjiI CACGTC 2 cut(s) 324, 832
AjnI CCWGG 1 cut(s) 886
AjuI GAANNNNNNNTTGG 2 cut(s) 215, 247
AluBI AGCT 2 cut(s) 542, 688
AluI AGCT 2 cut(s) 542, 688
Alw21I GWGCWC 1 cut(s) 802
Alw26I GTCTC 3 cut(s) 252, 399, 863
Alw44I GTGCAC 1 cut(s) 798
Aor51HI AGCGCT 1 cut(s) 227
AoxI GGCC 5 cut(s) 3, 189, 274, 327, 841
ApaLI GTGCAC 1 cut(s) 798
ApeKI GCWGC 1 cut(s) 46
ApoI RAATTY 1 cut(s) 458
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
Asp700I GAANNNNTTC 1 cut(s) 413
Asp718I GGTACC 1 cut(s) 582
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 259
AvaII GGWCC 1 cut(s) 259
AxyI CCTNAGG 1 cut(s) 680
BaeGI GKGCMC 1 cut(s) 802
BalI TGGCCA 2 cut(s) 5, 276
BanI GGYRCC 1 cut(s) 582
BarI GAAGNNNNNNTAC 4 cut(s) 677, 707, 709, 739
Bbv12I GWGCWC 1 cut(s) 802
BbvI GCAGC 1 cut(s) 33
BccI CCATC 7 cut(s) 92, 103, 173, 299, 502, 605, 664
BceAI ACGGC 2 cut(s) 187, 856
BciT130I CCWGG 1 cut(s) 888
BclI TGATCA 1 cut(s) 814
BcoDI GTCTC 3 cut(s) 252, 399, 863
BfmI CTRYAG 1 cut(s) 670
BfoI RGCGCY 1 cut(s) 229
BglI GCCNNNNNGGC 1 cut(s) 514
BisI GCNGC 2 cut(s) 12, 47
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 2 cut(s) 13, 48
Bme1390I CCNGG 1 cut(s) 888
Bme18I GGWCC 1 cut(s) 259
BmgBI CACGTC 2 cut(s) 324, 832
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 4 cut(s) 184, 261, 584, 677
BmrFI CCNGG 1 cut(s) 888
Bpu1102I GCTNAGC 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 674
Bsa29I ATCGAT 2 cut(s) 268, 912
BsaAI YACGTR 2 cut(s) 523, 919
BsaBI GATNNNNATC 1 cut(s) 816
BsaJI CCNNGG 4 cut(s) 186, 372, 838, 887
BsaXI ACNNNNNCTCC 6 cut(s) 104, 134, 185, 203, 215, 233
Bsc4I CCNNNNNNNGG 5 cut(s) 136, 347, 397, 427, 739
Bse118I RCCGGY 1 cut(s) 754
Bse1I ACTGG 1 cut(s) 68
Bse21I CCTNAGG 1 cut(s) 680
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 1 cut(s) 888
BseCI ATCGAT 2 cut(s) 268, 912
BseDI CCNNGG 4 cut(s) 186, 372, 838, 887
BseGI GGATG 8 cut(s) 150, 184, 291, 406, 494, 558, 651, 817
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 5 cut(s) 136, 347, 397, 427, 739
BseMII CTCAG 5 cut(s) 371, 410, 501, 671, 866
BseNI ACTGG 1 cut(s) 68
BseSI GKGCMC 1 cut(s) 802
BseX3I CGGCCG 1 cut(s) 327
BseXI GCAGC 1 cut(s) 33
BseYI CCCAGC 2 cut(s) 136, 562
Bsh1285I CGRYCG 1 cut(s) 330
BshFI GGCC 5 cut(s) 5, 191, 276, 329, 843
BshNI GGYRCC 1 cut(s) 582
BshVI ATCGAT 2 cut(s) 268, 912
BsiEI CGRYCG 1 cut(s) 330
BsiHKAI GWGCWC 1 cut(s) 802
BsiSI CCGG 1 cut(s) 755
BslFI GGGAC 1 cut(s) 547
BslI CCNNNNNNNGG 5 cut(s) 136, 347, 397, 427, 739
BsmAI GTCTC 3 cut(s) 252, 399, 863
BsmFI GGGAC 1 cut(s) 547
BsnI GGCC 5 cut(s) 5, 191, 276, 329, 843
Bsp1286I GDGCHC 1 cut(s) 802
Bsp143I GATC 5 cut(s) 265, 269, 814, 909, 913
Bsp1720I GCTNAGC 1 cut(s) 357
Bsp19I CCATGG 1 cut(s) 372
BspACI CCGC 3 cut(s) 9, 12, 387
BspANI GGCC 5 cut(s) 5, 191, 276, 329, 843
BspCNI CTCAG 5 cut(s) 370, 409, 500, 672, 867
BspDI ATCGAT 2 cut(s) 268, 912
BspLI GGNNCC 4 cut(s) 184, 261, 584, 677
BspT107I GGYRCC 1 cut(s) 582
BsrFI RCCGGY 1 cut(s) 754
BsrI ACTGG 1 cut(s) 68
BssAI RCCGGY 1 cut(s) 754
BssECI CCNNGG 4 cut(s) 186, 372, 838, 887
BssMI GATC 5 cut(s) 265, 269, 814, 909, 913
BssT1I CCWWGG 1 cut(s) 372
Bst2UI CCWGG 1 cut(s) 888
Bst4CI ACNGT 2 cut(s) 65, 766
BstBAI YACGTR 2 cut(s) 523, 919
BstC8I GCNNGC 2 cut(s) 363, 756
BstDEI CTNAG 5 cut(s) 357, 396, 487, 680, 875
BstDSI CCRYGG 2 cut(s) 372, 838
BstENI CCTNNNNNAGG 1 cut(s) 395
BstF5I GGATG 8 cut(s) 150, 184, 291, 406, 494, 558, 651, 817
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 5 cut(s) 268, 272, 817, 912, 916
BstMAI GTCTC 3 cut(s) 252, 399, 863
BstMBI GATC 5 cut(s) 265, 269, 814, 909, 913
BstMCI CGRYCG 1 cut(s) 330
BstMWI GCNNNNNNNGC 5 cut(s) 11, 180, 362, 514, 572
BstNI CCWGG 1 cut(s) 888
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 1 cut(s) 886
BstSFI CTRYAG 1 cut(s) 670
BstSLI GKGCMC 1 cut(s) 802
BstSNI TACGTA 1 cut(s) 523
BstV1I GCAGC 1 cut(s) 33
BstZI CGGCCG 1 cut(s) 327
Bsu15I ATCGAT 2 cut(s) 268, 912
Bsu36I CCTNAGG 1 cut(s) 680
BsuRI GGCC 5 cut(s) 5, 191, 276, 329, 843
BsuTUI ATCGAT 2 cut(s) 268, 912
BtgI CCRYGG 2 cut(s) 372, 838
BtrI CACGTC 2 cut(s) 324, 832
BtsCI GGATG 8 cut(s) 150, 184, 291, 406, 494, 558, 651, 817
BtsIMutI CAGTG 2 cut(s) 495, 872
Cac8I GCNNGC 2 cut(s) 363, 756
CfoI GCGC 1 cut(s) 228
Cfr10I RCCGGY 1 cut(s) 754
Cfr13I GGNCC 1 cut(s) 259
ClaI ATCGAT 2 cut(s) 268, 912
CseI GACGC 1 cut(s) 626
Csp6I GTAC 7 cut(s) 524, 583, 694, 715, 760, 781, 835
CviAII CATG 7 cut(s) 74, 133, 343, 373, 427, 734, 827
CviQI GTAC 7 cut(s) 524, 583, 694, 715, 760, 781, 835
DdeI CTNAG 5 cut(s) 357, 396, 487, 680, 875
DpnI GATC 5 cut(s) 267, 271, 816, 911, 915
DpnII GATC 5 cut(s) 265, 269, 814, 909, 913
DraIII CACNNNGTG 1 cut(s) 256
DrdI GACNNNNNNGTC 1 cut(s) 63
DseDI GACNNNNNNGTC 1 cut(s) 63
EaeI YGGCCR 4 cut(s) 3, 274, 327, 841
EagI CGGCCG 1 cut(s) 327
EclXI CGGCCG 1 cut(s) 327
Eco105I TACGTA 1 cut(s) 523
Eco130I CCWWGG 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 259
Eco47III AGCGCT 1 cut(s) 227
Eco52I CGGCCG 1 cut(s) 327
Eco81I CCTNAGG 1 cut(s) 680
EcoNI CCTNNNNNAGG 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 886
EcoT14I CCWWGG 1 cut(s) 372
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 7 cut(s) 77, 136, 346, 376, 430, 737, 830
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FaqI GGGAC 1 cut(s) 547
FatI CATG 7 cut(s) 73, 132, 342, 372, 426, 733, 826
FbaI TGATCA 1 cut(s) 814
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 2 cut(s) 12, 47
FokI GGATG 8 cut(s) 157, 191, 278, 413, 481, 545, 638, 804
Fsp4HI GCNGC 2 cut(s) 12, 47
GlaI GCGC 1 cut(s) 227
GluI GCNGC 2 cut(s) 12, 47
GsaI CCCAGC 2 cut(s) 140, 566
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 5 cut(s) 5, 191, 276, 329, 843
HapII CCGG 1 cut(s) 755
HgaI GACGC 1 cut(s) 626
HhaI GCGC 1 cut(s) 228
Hin1II CATG 7 cut(s) 77, 136, 346, 376, 430, 737, 830
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HinfI GANTC 3 cut(s) 57, 245, 414
HpaII CCGG 1 cut(s) 755
Hpy166II GTNNAC 5 cut(s) 259, 439, 762, 800, 835
Hpy188I TCNGA 4 cut(s) 187, 296, 316, 457
Hpy188III TCNNGA 3 cut(s) 398, 604, 793
Hpy8I GTNNAC 5 cut(s) 259, 439, 762, 800, 835
Hpy99I CGWCG 3 cut(s) 328, 617, 620
HpyAV CCTTC 6 cut(s) 50, 292, 578, 733, 737, 850
HpyCH4III ACNGT 2 cut(s) 65, 766
HpyCH4IV ACGT 4 cut(s) 323, 522, 831, 918
HpyCH4V TGCA 3 cut(s) 548, 737, 800
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 180, 362, 514, 572
HpyF3I CTNAG 5 cut(s) 357, 396, 487, 680, 875
HpySE526I ACGT 4 cut(s) 323, 522, 831, 918
Hsp92II CATG 7 cut(s) 77, 136, 346, 376, 430, 737, 830
HspAI GCGC 1 cut(s) 226
KpnI GGTACC 1 cut(s) 586
KroI GCCGGC 1 cut(s) 754
KroNI GCCGGC 1 cut(s) 756
Ksp22I TGATCA 1 cut(s) 814
Kzo9I GATC 5 cut(s) 265, 269, 814, 909, 913
LmnI GCTCC 1 cut(s) 188
Lsp1109I GCAGC 1 cut(s) 33
MaeII ACGT 4 cut(s) 323, 522, 831, 918
MalI GATC 5 cut(s) 267, 271, 816, 911, 915
MboI GATC 5 cut(s) 265, 269, 814, 909, 913
MboII GAAGA 4 cut(s) 160, 223, 226, 592
MhlI GDGCHC 1 cut(s) 802
MlsI TGGCCA 2 cut(s) 5, 276
MluCI AATT 1 cut(s) 458
MluNI TGGCCA 2 cut(s) 5, 276
MlyI GAGTC 2 cut(s) 51, 254
MmeI TCCRAC 1 cut(s) 294
Mox20I TGGCCA 2 cut(s) 5, 276
MroNI GCCGGC 1 cut(s) 754
MroXI GAANNNNTTC 1 cut(s) 413
MscI TGGCCA 2 cut(s) 5, 276
MslI CAYNNNNRTG 1 cut(s) 905
Msp20I TGGCCA 2 cut(s) 5, 276
MspA1I CMGCKG 1 cut(s) 542
MspI CCGG 1 cut(s) 755
MspR9I CCNGG 1 cut(s) 888
MvaI CCWGG 1 cut(s) 888
MwoI GCNNNNNNNGC 5 cut(s) 11, 180, 362, 514, 572
NaeI GCCGGC 1 cut(s) 756
NcoI CCATGG 1 cut(s) 372
NdeII GATC 5 cut(s) 265, 269, 814, 909, 913
NgoMIV GCCGGC 1 cut(s) 754
NlaIII CATG 7 cut(s) 77, 136, 346, 376, 430, 737, 830
NlaIV GGNNCC 4 cut(s) 184, 261, 584, 677
NspI RCATGY 1 cut(s) 136
PcsI WCGNNNNNNNCGW 2 cut(s) 320, 609
PdiI GCCGGC 1 cut(s) 756
PdmI GAANNNNTTC 1 cut(s) 413
PfeI GAWTC 1 cut(s) 414
PflMI CCANNNNNTGG 1 cut(s) 136
PkrI GCNGC 2 cut(s) 13, 48
PleI GAGTC 2 cut(s) 51, 253
PpsI GAGTC 2 cut(s) 51, 253
Ppu21I YACGTR 2 cut(s) 523, 919
Psp6I CCWGG 1 cut(s) 886
PspFI CCCAGC 2 cut(s) 136, 562
PspGI CCWGG 1 cut(s) 886
PspN4I GGNNCC 4 cut(s) 184, 261, 584, 677
PspPI GGNCC 1 cut(s) 259
PvuII CAGCTG 1 cut(s) 542
RsaI GTAC 7 cut(s) 525, 584, 695, 716, 761, 782, 836
RsaNI GTAC 7 cut(s) 524, 583, 694, 715, 760, 781, 835
RseI CAYNNNNRTG 1 cut(s) 905
SatI GCNGC 2 cut(s) 12, 47
Sau3AI GATC 5 cut(s) 265, 269, 814, 909, 913
Sau96I GGNCC 1 cut(s) 259
SchI GAGTC 2 cut(s) 51, 254
ScrFI CCNGG 1 cut(s) 888
SduI GDGCHC 1 cut(s) 802
SfcI CTRYAG 1 cut(s) 670
SinI GGWCC 1 cut(s) 259
SmiMI CAYNNNNRTG 1 cut(s) 905
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
SnaBI TACGTA 1 cut(s) 523
Sse9I AATT 1 cut(s) 458
SsiI CCGC 3 cut(s) 9, 12, 387
StyD4I CCNGG 1 cut(s) 886
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 2 cut(s) 65, 766
TaiI ACGT 4 cut(s) 326, 525, 834, 921
TaqI TCGA 7 cut(s) 126, 268, 603, 612, 659, 786, 912
TasI AATT 1 cut(s) 458
TatI WGTACW 1 cut(s) 693
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 1 cut(s) 414
TscAI CASTG 2 cut(s) 495, 879
TseI GCWGC 1 cut(s) 46
TspDTI ATGAA 3 cut(s) 161, 359, 422
TspRI CASTG 2 cut(s) 495, 879
Van91I CCANNNNNTGG 1 cut(s) 136
VneI GTGCAC 1 cut(s) 798
VpaK11BI GGWCC 1 cut(s) 259
XagI CCTNNNNNAGG 1 cut(s) 395
XapI RAATTY 1 cut(s) 458
XceI RCATGY 1 cut(s) 136
XmiI GTMKAC 1 cut(s) 438
XmnI GAANNNNTTC 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.