pycom03g16120

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0015382
Physical Location & Seq
Reverse (-)
17286682 .. 17287519
838 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 741 bp
ATGCTGCTACTGGCAAACTACCTATTTTCGGCTTTCTTCCCCTACTCTGTTAAAACGCTCAAATGGCTTTCAACCTTCTCAAAATCCACACAAAAACCATCAAAACTCCAAACAACCCCACCCCTAACCCACACCCCCCCCCCCCCCCCCCCCCCCAAAAAAAAATCAATTCCCATTCCCATTTCCCAAACACCACCAAAACACTTGCATCTCCATCCCTTCAACCCTCCCCCCAAACCCAAAATTACCACTCTATCCGCAATCTGCTCCTCCTTGTCCTCCTCCCCCACCCCTCCAACCTCCAAAGAAGCCGCCATCAAAAAAGCCAAAACCTGCCTCTCCACCACCCTCTCCAAGCTCCTCAACAATCTTCGCCTCACCACCAAGCTCAAGAAGCCCAAACAACCCCGATTCCGACTCGAAATCCCCCTCGCCGACGACTCCCCGGAATCCCTCTCCCACCTCGCCCTCCAGCTCTTCCAAGACCTACCCATAAAACGAATATCCTCCAAAGTCAACGTCTTGATCGTATGGTCAAACGCCGCCTTCGAAGAAGCCGCATTGAAAGCCTTTGGGGCTTGCCCTTCGAGCCCGGTTGAGCATTCGGACATTTCCTCCATCGCCGACGATAATACCGGAAATTTGAATTCTGCTGACGTGGCGGTGTTTTTGGCGCCAGAGGGAACCCAACTGGCGGTTACAAAAACGGTCACTGATTTGTTGTTCCGTGGCTGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

247

Amino Acids

26.9

Weight (kDa)

9.82

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1995 PF09353 99 - 238 7.2e-07 Domain of unknown function (DUF1995)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20680
fragaria_vesca FvH4_3g21740
malus_domestica MD03G1212500.v1.1 MD11G1227700.v1.1
prunus_persica Prupe.4G201600_v2.0.a1
pyrus_communis pycom03g16120
rosa_chinensis RchiOBHm_Chr5g0037211
rosa_laevigata RLG00000033765
rosa_multiflora Rmu_co8240257.1_g000001 Rmu_sc0006633.1_g000004
rosa_roxburghii Rroxscaffold_1G00043760
rosa_rugosa Rorug05G0163800
rosa_samantha Rh5BG255400 Rh5CG288100 Rh5DG263700
rosa_wichuraiana Rw5G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 673
AciI CCGC 6 cut(s) 258, 312, 543, 558, 662, 695
AcsI RAATTY 2 cut(s) 640, 646
AcyI GRCGYC 1 cut(s) 674
AfiI CCNNNNNNNGG 2 cut(s) 28, 694
AgsI TTSAA 4 cut(s) 72, 223, 565, 646
AjiI CACGTC 1 cut(s) 658
AluBI AGCT 3 cut(s) 358, 388, 475
AluI AGCT 3 cut(s) 358, 388, 475
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 2 cut(s) 640, 646
ArsI GACNNNNNNTTYG 2 cut(s) 504, 536
AspLEI GCGC 1 cut(s) 676
AsuC2I CCSGG 2 cut(s) 446, 593
AsuHPI GGTGA 1 cut(s) 370
AsuII TTCGAA 1 cut(s) 549
BanI GGYRCC 1 cut(s) 673
BanII GRGCYC 1 cut(s) 593
BccI CCATC 4 cut(s) 106, 222, 323, 626
BcnI CCSGG 2 cut(s) 446, 593
BfoI RGCGCY 1 cut(s) 677
BfuAI ACCTGC 1 cut(s) 341
BglI GCCNNNNNGGC 1 cut(s) 575
BisI GCNGC 4 cut(s) 5, 312, 543, 558
BlsI GCNGC 4 cut(s) 6, 313, 544, 559
Bme1390I CCNGG 2 cut(s) 446, 593
BmgBI CACGTC 1 cut(s) 658
BmiI GGNNCC 2 cut(s) 675, 685
BmrFI CCNGG 2 cut(s) 446, 593
BmsI GCATC 1 cut(s) 217
BpmI CTGGAG 1 cut(s) 455
Bpu14I TTCGAA 1 cut(s) 549
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 2 cut(s) 446, 593
BsaHI GRCGYC 1 cut(s) 674
BsaJI CCNNGG 2 cut(s) 444, 727
BsaWI WCCGGW 1 cut(s) 635
BsaXI ACNNNNNCTCC 2 cut(s) 599, 629
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 694
Bse1I ACTGG 2 cut(s) 15, 696
BseDI CCNNGG 2 cut(s) 444, 727
BseGI GGATG 1 cut(s) 214
BseLI CCNNNNNNNGG 2 cut(s) 28, 694
BseNI ACTGG 2 cut(s) 15, 696
BseRI GAGGAG 3 cut(s) 259, 271, 350
BshNI GGYRCC 1 cut(s) 673
BsiSI CCGG 3 cut(s) 446, 593, 636
BslI CCNNNNNNNGG 2 cut(s) 28, 694
BsmI GAATGC 1 cut(s) 601
Bsp119I TTCGAA 1 cut(s) 549
Bsp1286I GDGCHC 1 cut(s) 593
Bsp143I GATC 1 cut(s) 525
BspACI CCGC 6 cut(s) 258, 312, 543, 558, 662, 695
BspLI GGNNCC 2 cut(s) 675, 685
BspMI ACCTGC 1 cut(s) 341
BspQI GCTCTTC 1 cut(s) 482
BspT104I TTCGAA 1 cut(s) 549
BspT107I GGYRCC 1 cut(s) 673
BsrI ACTGG 2 cut(s) 15, 696
BssECI CCNNGG 2 cut(s) 444, 727
BssMI GATC 1 cut(s) 525
BssNI GRCGYC 1 cut(s) 674
Bst4CI ACNGT 1 cut(s) 709
Bst6I CTCTTC 1 cut(s) 482
BstACI GRCGYC 1 cut(s) 674
BstBI TTCGAA 1 cut(s) 549
BstC8I GCNNGC 1 cut(s) 580
BstDSI CCRYGG 1 cut(s) 727
BstF5I GGATG 1 cut(s) 214
BstH2I RGCGCY 1 cut(s) 677
BstHHI GCGC 1 cut(s) 676
BstKTI GATC 1 cut(s) 528
BstMBI GATC 1 cut(s) 525
BstMWI GCNNNNNNNGC 6 cut(s) 64, 394, 566, 575, 588, 659
BstSCI CCNGG 2 cut(s) 444, 591
BtgI CCRYGG 1 cut(s) 727
BtgZI GCGATG 1 cut(s) 604
BtrI CACGTC 1 cut(s) 658
BtsCI GGATG 1 cut(s) 214
BtsIMutI CAGTG 1 cut(s) 711
BveI ACCTGC 1 cut(s) 341
Cac8I GCNNGC 1 cut(s) 580
CfoI GCGC 1 cut(s) 676
DinI GGCGCC 1 cut(s) 675
DpnI GATC 1 cut(s) 527
DpnII GATC 1 cut(s) 525
Eam1104I CTCTTC 1 cut(s) 482
EarI CTCTTC 1 cut(s) 482
Eco24I GRGCYC 1 cut(s) 593
EcoRI GAATTC 1 cut(s) 646
EcoT38I GRGCYC 1 cut(s) 593
EgeI GGCGCC 1 cut(s) 675
EheI GGCGCC 1 cut(s) 675
FaiI YATR 2 cut(s) 494, 532
Fnu4HI GCNGC 4 cut(s) 5, 312, 543, 558
FokI GGATG 1 cut(s) 201
FriOI GRGCYC 1 cut(s) 593
Fsp4HI GCNGC 4 cut(s) 5, 312, 543, 558
GlaI GCGC 1 cut(s) 675
GluI GCNGC 4 cut(s) 5, 312, 543, 558
GsuI CTGGAG 1 cut(s) 455
HaeII RGCGCY 1 cut(s) 677
HapII CCGG 3 cut(s) 446, 593, 636
HhaI GCGC 1 cut(s) 676
Hin1I GRCGYC 1 cut(s) 674
Hin6I GCGC 1 cut(s) 674
HinP1I GCGC 1 cut(s) 674
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HinfI GANTC 4 cut(s) 411, 417, 440, 449
HpaII CCGG 3 cut(s) 446, 593, 636
HphI GGTGA 1 cut(s) 370
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 2 cut(s) 416, 607
Hpy188III TCNNGA 2 cut(s) 391, 523
Hpy8I GTNNAC 1 cut(s) 517
Hpy99I CGWCG 2 cut(s) 440, 629
HpyAV CCTTC 4 cut(s) 85, 229, 556, 594
HpyCH4III ACNGT 1 cut(s) 709
HpyCH4IV ACGT 2 cut(s) 519, 657
HpyCH4V TGCA 1 cut(s) 208
HpyF10VI GCNNNNNNNGC 6 cut(s) 64, 394, 566, 575, 588, 659
HpySE526I ACGT 2 cut(s) 519, 657
Hsp92I GRCGYC 1 cut(s) 674
HspAI GCGC 1 cut(s) 674
KasI GGCGCC 1 cut(s) 673
Kzo9I GATC 1 cut(s) 525
LguI GCTCTTC 1 cut(s) 482
LmnI GCTCC 2 cut(s) 272, 363
LpnPI CCDG 8 cut(s) 346, 459, 485, 606, 649, 677, 690, 718
LweI GCATC 1 cut(s) 217
MaeII ACGT 2 cut(s) 519, 657
MaeIII GTNAC 2 cut(s) 697, 709
MalI GATC 1 cut(s) 527
MboI GATC 1 cut(s) 525
MboII GAAGA 4 cut(s) 28, 362, 469, 563
MhlI GDGCHC 1 cut(s) 593
MluCI AATT 4 cut(s) 168, 243, 640, 646
Mly113I GGCGCC 1 cut(s) 674
MlyI GAGTC 2 cut(s) 411, 434
MmeI TCCRAC 2 cut(s) 320, 439
MseI TTAA 1 cut(s) 51
MspI CCGG 3 cut(s) 446, 593, 636
MspR9I CCNGG 2 cut(s) 446, 593
Mva1269I GAATGC 1 cut(s) 601
MwoI GCNNNNNNNGC 6 cut(s) 64, 394, 566, 575, 588, 659
NarI GGCGCC 1 cut(s) 674
NciI CCSGG 2 cut(s) 446, 593
NdeII GATC 1 cut(s) 525
NlaIV GGNNCC 2 cut(s) 675, 685
NmuCI GTSAC 1 cut(s) 709
NspV TTCGAA 1 cut(s) 549
PciSI GCTCTTC 1 cut(s) 482
PcsI WCGNNNNNNNCGW 2 cut(s) 525, 546
PctI GAATGC 1 cut(s) 601
PfeI GAWTC 2 cut(s) 411, 449
PkrI GCNGC 4 cut(s) 6, 313, 544, 559
PleI GAGTC 2 cut(s) 411, 434
PluTI GGCGCC 1 cut(s) 677
PpsI GAGTC 2 cut(s) 411, 434
PspN4I GGNNCC 2 cut(s) 675, 685
SapI GCTCTTC 1 cut(s) 482
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 4 cut(s) 5, 312, 543, 558
Sau3AI GATC 1 cut(s) 525
SchI GAGTC 2 cut(s) 411, 434
ScrFI CCNGG 2 cut(s) 446, 593
SduI GDGCHC 1 cut(s) 593
SfaNI GCATC 1 cut(s) 217
SfoI GGCGCC 1 cut(s) 675
SfuI TTCGAA 1 cut(s) 549
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 4 cut(s) 168, 243, 640, 646
SsiI CCGC 6 cut(s) 258, 312, 543, 558, 662, 695
SspDI GGCGCC 1 cut(s) 673
StyD4I CCNGG 2 cut(s) 444, 591
TaaI ACNGT 1 cut(s) 709
TaiI ACGT 2 cut(s) 522, 660
TaqI TCGA 3 cut(s) 420, 549, 587
TasI AATT 4 cut(s) 168, 243, 640, 646
TauI GCSGC 3 cut(s) 314, 545, 560
TfiI GAWTC 2 cut(s) 411, 449
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TscAI CASTG 1 cut(s) 718
TseFI GTSAC 1 cut(s) 709
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 709
TspGWI ACGGA 1 cut(s) 716
TspRI CASTG 1 cut(s) 718
XapI RAATTY 2 cut(s) 640, 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.