pycom03g17190

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0014323
Physical Location & Seq
Forward (+)
18370689 .. 18372681
1993 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 555 bp
ATGGCATCCAAGCTGTTACTGATTACAGTGTTCATCCTGAATGTCATTGCTTTTGGGCTCGCTGTTGCAGCTGAGCAGAGGAGAAGCTCTGCGAATGTCGTTCAGAATACTGAGTTAAATTACAACTATTGTGTTTATGAGTCAGACATCGCAACTGGCTACGGGGTTGGTGCATTTTTGTTCTTCTTGGTAGGTCAGGTCATTATAATGGTGGCAAGCCGATGCTTCTGCTGCGGGAAGCCATTGAAACCTGGAGGGTCAAGAGCCTGGGCAGTTATCCTTTTCATAGTCTGCTGGGTCTTTTTCTTAATTGCTGAGGCATGTTTGCTGGCGGGTTCAGTGAGGAATGCCTACCACACCAAGTACAGGACCATTTTCGGCCAAAGCCCTCCAGATTGCCAGCAACTGAGAAAGGGAGTTTTCGGTGCAGGAGCAGCTTTCATTTTCCTCTCCACAATAGTTTCTGAGTTCTACTATGTTTGCTATTCCAGAGCCAGGGAAAGCTTCCAGCCGTACGGCGGAGAGGCTGCTGTTGGTCTCGGAACCTTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

185

Amino Acids

20.15

Weight (kDa)

8.94

Isoelectric Point (pI)

31.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 58 - 152 2.4e-31 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014323)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27435
fragaria_vesca FvH4_3g20620
malus_domestica MD03G1223600.v1.1 MD11G1241500.v1.1
prunus_persica Prupe.4G185900_v2.0.a1
pyrus_communis pycom03g17190 pycom11g21310
rosa_chinensis RchiOBHm_Chr5g0034641
rosa_laevigata RLG00000033562
rosa_multiflora Rmu_sc0002460.1_g000087
rosa_roxburghii Rroxscaffold_1G00045680
rosa_rugosa Rorug05G0147400 Rorug05G0147400
rosa_samantha Rh5AG239400 Rh5BG240100 Rh5DG248300
rosa_wichuraiana Rw5G021940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 206
AciI CCGC 3 cut(s) 234, 332, 519
AcoI YGGCCR 1 cut(s) 379
AfaI GTAC 2 cut(s) 365, 515
AfiI CCNNNNNNNGG 3 cut(s) 366, 495, 518
AgsI TTSAA 1 cut(s) 247
AjnI CCWGG 3 cut(s) 250, 266, 494
AluBI AGCT 5 cut(s) 13, 71, 87, 437, 504
AluI AGCT 5 cut(s) 13, 71, 87, 437, 504
Alw26I GTCTC 1 cut(s) 542
AlwNI CAGNNNCTG 1 cut(s) 406
AoxI GGCC 1 cut(s) 379
ApeKI GCWGC 4 cut(s) 68, 231, 434, 527
AspS9I GGNCC 1 cut(s) 369
AvaII GGWCC 1 cut(s) 369
BanII GRGCYC 1 cut(s) 60
BbvCI CCTCAGC 1 cut(s) 315
BbvI GCAGC 4 cut(s) 80, 218, 446, 514
BceAI ACGGC 2 cut(s) 496, 532
BcgI CGANNNNNNTGC 2 cut(s) 210, 244
BciT130I CCWGG 3 cut(s) 252, 268, 496
BcoDI GTCTC 1 cut(s) 542
BisI GCNGC 4 cut(s) 69, 232, 435, 528
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 4 cut(s) 70, 233, 436, 529
Bme1390I CCNGG 3 cut(s) 252, 268, 496
Bme18I GGWCC 1 cut(s) 369
BmgT120I GGNCC 1 cut(s) 369
BmiI GGNNCC 1 cut(s) 544
BmrFI CCNGG 3 cut(s) 252, 268, 496
BmsI GCATC 2 cut(s) 14, 212
BpmI CTGGAG 2 cut(s) 273, 375
Bpu10I CCTNAGC 1 cut(s) 315
Bpu1102I GCTNAGC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 542
BsaJI CCNNGG 2 cut(s) 267, 495
Bsc4I CCNNNNNNNGG 3 cut(s) 366, 495, 518
Bse1I ACTGG 1 cut(s) 160
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 3 cut(s) 252, 268, 496
BseDI CCNNGG 2 cut(s) 267, 495
BseGI GGATG 2 cut(s) 5, 33
BseLI CCNNNNNNNGG 3 cut(s) 366, 495, 518
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 5 cut(s) 63, 102, 306, 398, 456
BseNI ACTGG 1 cut(s) 160
BseRI GAGGAG 1 cut(s) 94
BseXI GCAGC 4 cut(s) 80, 218, 446, 514
BseYI CCCAGC 1 cut(s) 294
BsgI GTGCAG 1 cut(s) 447
BshFI GGCC 1 cut(s) 381
BsiWI CGTACG 1 cut(s) 513
BslI CCNNNNNNNGG 3 cut(s) 366, 495, 518
BsmAI GTCTC 1 cut(s) 542
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 1 cut(s) 381
Bso31I GGTCTC 1 cut(s) 542
Bsp1286I GDGCHC 1 cut(s) 60
Bsp1720I GCTNAGC 1 cut(s) 72
BspACI CCGC 3 cut(s) 234, 332, 519
BspANI GGCC 1 cut(s) 381
BspCNI CTCAG 5 cut(s) 64, 103, 307, 399, 457
BspLI GGNNCC 1 cut(s) 544
BspTNI GGTCTC 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 1 cut(s) 160
BssECI CCNNGG 2 cut(s) 267, 495
Bst2UI CCWGG 3 cut(s) 252, 268, 496
Bst4CI ACNGT 1 cut(s) 28
BstC8I GCNNGC 4 cut(s) 60, 217, 330, 401
BstDEI CTNAG 5 cut(s) 72, 111, 315, 407, 465
BstF5I GGATG 2 cut(s) 5, 33
BstMAI GTCTC 1 cut(s) 542
BstMWI GCNNNNNNNGC 3 cut(s) 68, 231, 434
BstNI CCWGG 3 cut(s) 252, 268, 496
BstNSI RCATGY 1 cut(s) 324
BstSCI CCNGG 3 cut(s) 250, 266, 494
BstV1I GCAGC 4 cut(s) 80, 218, 446, 514
BsuRI GGCC 1 cut(s) 381
BtgZI GCGATG 1 cut(s) 133
BtsCI GGATG 2 cut(s) 5, 33
BtsIMutI CAGTG 2 cut(s) 33, 345
Cac8I GCNNGC 4 cut(s) 60, 217, 330, 401
CaiI CAGNNNCTG 1 cut(s) 406
Cfr13I GGNCC 1 cut(s) 369
Csp6I GTAC 2 cut(s) 364, 514
CviAII CATG 1 cut(s) 321
CviQI GTAC 2 cut(s) 364, 514
DdeI CTNAG 5 cut(s) 72, 111, 315, 407, 465
DraI TTTAAA 1 cut(s) 550
EaeI YGGCCR 1 cut(s) 379
EciI GGCGGA 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 542
Eco47I GGWCC 1 cut(s) 369
EcoRII CCWGG 3 cut(s) 250, 266, 494
EcoT38I GRGCYC 1 cut(s) 60
FaeI CATG 1 cut(s) 324
FaiI YATR 5 cut(s) 138, 206, 287, 322, 477
FatI CATG 1 cut(s) 320
FauI CCCGC 2 cut(s) 227, 325
Fnu4HI GCNGC 4 cut(s) 69, 232, 435, 528
FokI GGATG 1 cut(s) 20
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 4 cut(s) 69, 232, 435, 528
GluI GCNGC 4 cut(s) 69, 232, 435, 528
GsaI CCCAGC 1 cut(s) 298
GsuI CTGGAG 2 cut(s) 273, 375
HaeIII GGCC 1 cut(s) 381
Hin1II CATG 1 cut(s) 324
HindIII AAGCTT 1 cut(s) 502
HinfI GANTC 1 cut(s) 140
Hpy188I TCNGA 4 cut(s) 105, 145, 466, 542
Hpy188III TCNNGA 4 cut(s) 37, 261, 392, 489
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4V TGCA 3 cut(s) 68, 173, 428
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 231, 434
HpyF3I CTNAG 5 cut(s) 72, 111, 315, 407, 465
Hsp92II CATG 1 cut(s) 324
LmnI GCTCC 1 cut(s) 431
Lsp1109I GCAGC 4 cut(s) 80, 218, 446, 514
LweI GCATC 2 cut(s) 14, 212
MaeIII GTNAC 1 cut(s) 15
MboII GAAGA 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 60
MluCI AATT 2 cut(s) 118, 309
MlyI GAGTC 1 cut(s) 149
MnlI CCTC 7 cut(s) 72, 248, 310, 336, 399, 458, 517
MseI TTAA 3 cut(s) 116, 308, 549
MslI CAYNNNNRTG 1 cut(s) 206
MspA1I CMGCKG 1 cut(s) 71
MspR9I CCNGG 3 cut(s) 252, 268, 496
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 3 cut(s) 252, 268, 496
MwoI GCNNNNNNNGC 3 cut(s) 68, 231, 434
NlaIII CATG 1 cut(s) 324
NlaIV GGNNCC 1 cut(s) 544
NspI RCATGY 1 cut(s) 324
PctI GAATGC 1 cut(s) 352
Pfl23II CGTACG 1 cut(s) 513
PkrI GCNGC 4 cut(s) 70, 233, 436, 529
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PsiI TTATAA 1 cut(s) 206
Psp6I CCWGG 3 cut(s) 250, 266, 494
PspFI CCCAGC 1 cut(s) 294
PspGI CCWGG 3 cut(s) 250, 266, 494
PspLI CGTACG 1 cut(s) 513
PspN4I GGNNCC 1 cut(s) 544
PspPI GGNCC 1 cut(s) 369
PstNI CAGNNNCTG 1 cut(s) 406
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 2 cut(s) 365, 515
RsaNI GTAC 2 cut(s) 364, 514
RseI CAYNNNNRTG 1 cut(s) 206
SaqAI TTAA 3 cut(s) 116, 308, 549
SatI GCNGC 4 cut(s) 69, 232, 435, 528
Sau96I GGNCC 1 cut(s) 369
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 3 cut(s) 252, 268, 496
SduI GDGCHC 1 cut(s) 60
SetI ASST 9 cut(s) 15, 73, 89, 196, 201, 253, 439, 506, 548
SfaNI GCATC 2 cut(s) 14, 212
SinI GGWCC 1 cut(s) 369
SmiMI CAYNNNNRTG 1 cut(s) 206
Sse9I AATT 2 cut(s) 118, 309
SsiI CCGC 3 cut(s) 234, 332, 519
StyD4I CCNGG 3 cut(s) 250, 266, 494
TaaI ACNGT 1 cut(s) 28
TasI AATT 2 cut(s) 118, 309
TatI WGTACW 1 cut(s) 363
Tru1I TTAA 3 cut(s) 116, 308, 549
Tru9I TTAA 3 cut(s) 116, 308, 549
TscAI CASTG 2 cut(s) 33, 345
TseI GCWGC 4 cut(s) 68, 231, 434, 527
TspDTI ATGAA 3 cut(s) 22, 274, 430
TspRI CASTG 2 cut(s) 33, 345
VpaK11BI GGWCC 1 cut(s) 369
XceI RCATGY 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.