pycom03g17520

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003383
Physical Location & Seq
Reverse (-)
18648353 .. 18652491
4139 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1731 bp
ATGACTGGAGGGTCATTCGGTCTACGTACAGGGAGTTATGGATCACTGCTACAACAACAACCGATACAGAATGGTCTTTCACATAATCAGCCAACACCTATTCTTACCCGCAAGTCTTCAAAGTTGCTTCTTTCCGGTTCAAGGGAGAAGGAAAGAGTTTTGCTCTTTGTCTGTAGATTCTTGGGTCGAAAGCGAGTCACAATGCTTCTTTTGGTTGTCCTTGCTCTTTTGGTTTTCGTATTCAATTCCTTCACTGTAAATAGAGAAAGCACTAATGTCATTATGACTCAACATAGTGAAAATGTGATACCTTATCGCGAGGAGATGTCAAGTAGTTCCGTAACTTTTGAAGACATGGAAGACAGACATGACATTAAACTTCCTTCTCCCCATTCCCTTGCTCAACCATTTGCTGTTGTTCGGGGAAATGCCCCTCTTTTGCCTCACCACTGTGAAAATTTTGCATTTCCCCCTCCTCCTCCTCCAACCGATCGAAAACGCTATGGACCACGCCCATGCCCAGTTTGCTATCTACCTGTGGAGCAGGCTATAGCTAGTATGCCAAGCTACCCCTCAGAATCACCTGTCCTACATAATTTGACATATGTTTATGATGAAAATCCTACTAGAGTTGAACCACATAGAGGTTCTGAATTTGGTGGATTTCCAACTCTAAAGCAGAGAAATGACTCTTATGATATAAAAGAATCCATGATGGTCCACTGTGGATTTGTGAACGCAAGCAAACCTGGTCACCGGTCTGGATTTGATGTTGATGAAGCTGACCTTATGGAGTTGGAGATGTACCATGATGTCATTGTTGCATCAGCTGTCTTTGGAAACTATGACATAATACAACAACCAAAGAACATCAGTGAGTTTTCGAGGAAAAATGTTCCATTCTACTTGTTTATTGACGAAGAGACAGAAGCTTATATGAAGAATTATAGTGTCCTGGGAGACAGTAAGAGGATAGGGTTGTGGAGAATTATTGTTGTCCGCAACGTTCCTTACAGTGATGCAAGGCGTAATGGAAAGATTCCGAAGCTTCTATTGCATAGGATTTTCCCCAATGTCAGATACTCTATATGGATTGATGGAAAGCTTCGGCTTGTGGTGGATCCTTTTCAAGTCCTTGAGAGGTTCTTGTGGCGTCAGAATGCAAACTTTGCAATTTCAAGGCACTATAAACGGTATGATGTGTTTGTAGAAGCTGAAGCTAACAAAGCTGCTGGAAAGTATGATAATTCCACCATTAATGAGCAGATTGACTTCTATATTAAGGAGGGTTTGCAACCTTATTCAGAGGCTAAGTTTCCAATAACGAGTGATGTTCCTGAAGGTTGTGTTATCATAAAGGAACACATTCCCATCACAAATTTATTTACCTGCAATTGGTTCAACGAAGTTGATCGTTTTACCTCCAGGGATCAGTTAAGCTTCTCCACAGTGAGGGATAGAATCATGGGAAAAGTTGATTGGAGCATTAATATGTTTTTGGACTGTGAAAGGCGGAATTTTGTAATTCAGGCATACCACAGAGAGTTACTGGAGCACATGCCTCCTCCCCCACCTCCTAGGGTTATTGTTCGTCGTCCGCCATCTTTGTCTCATGGTGCCACATCTGTAAAGACTCCAATCGGGAAGAAGATATCTCAAAAGCGTGGACGAGGAGATAAGAAGCGCCATCGTAAGGTTGCTGGTGGTAGTAGAGACAGCAAGACATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

577

Amino Acids

66.02

Weight (kDa)

9.3

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 200 - 513 1.3e-143 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
Acc36I ACCTGC 1 cut(s) 1397
AccB1I GGYRCC 1 cut(s) 1616
AccI GTMKAC 1 cut(s) 22
AccII CGCG 1 cut(s) 318
AciI CCGC 4 cut(s) 109, 1000, 1513, 1598
AclI AACGTT 1 cut(s) 1005
AclWI GGATC 4 cut(s) 49, 1115, 1128, 1437
AcsI RAATTY 4 cut(s) 457, 653, 1378, 1516
AcuI CTGAAG 2 cut(s) 1236, 1359
AcyI GRCGYC 1 cut(s) 1153
AfaI GTAC 2 cut(s) 28, 806
AfiI CCNNNNNNNGG 5 cut(s) 141, 644, 1395, 1452, 1693
AgeI ACCGGT 1 cut(s) 756
AgsI TTSAA 8 cut(s) 120, 141, 244, 350, 635, 1130, 1179, 1402
AjnI CCWGG 3 cut(s) 748, 954, 1424
AjuI GAANNNNNNNTTGG 2 cut(s) 1462, 1494
AleI CACNNNNGTG 1 cut(s) 450
Alw21I GWGCWC 1 cut(s) 1557
Alw26I GTCTC 4 cut(s) 917, 954, 1614, 1707
AlwI GGATC 4 cut(s) 49, 1115, 1128, 1437
ApeKI GCWGC 1 cut(s) 1229
ApoI RAATTY 4 cut(s) 457, 653, 1378, 1516
AseI ATTAAT 2 cut(s) 1257, 1488
AsiGI ACCGGT 1 cut(s) 756
Asp700I GAANNNNTTC 1 cut(s) 1365
AspA2I CCTAGG 1 cut(s) 1577
AspLEI GCGC 1 cut(s) 1686
AspS9I GGNCC 2 cut(s) 506, 718
AsuHPI GGTGA 3 cut(s) 437, 573, 746
AvaII GGWCC 2 cut(s) 506, 718
AvrII CCTAGG 1 cut(s) 1577
BamHI GGATCC 1 cut(s) 1120
BanI GGYRCC 1 cut(s) 1616
BbsI GAAGAC 3 cut(s) 108, 357, 366
Bbv12I GWGCWC 1 cut(s) 1557
BbvI GCAGC 1 cut(s) 1216
BccI CCATC 5 cut(s) 709, 1091, 1379, 1609, 1695
BciT130I CCWGG 3 cut(s) 750, 956, 1426
BcoDI GTCTC 4 cut(s) 917, 954, 1614, 1707
BfaI CTAG 3 cut(s) 555, 627, 1578
BfmI CTRYAG 2 cut(s) 172, 549
BfoI RGCGCY 1 cut(s) 1687
BfuAI ACCTGC 1 cut(s) 1397
BisI GCNGC 1 cut(s) 1230
BlnI CCTAGG 1 cut(s) 1577
BlsI GCNGC 1 cut(s) 1231
Bme1390I CCNGG 3 cut(s) 750, 956, 1426
Bme18I GGWCC 2 cut(s) 506, 718
BmgT120I GGNCC 2 cut(s) 506, 718
BmiI GGNNCC 2 cut(s) 1122, 1618
BmrFI CCNGG 3 cut(s) 750, 956, 1426
BmrI ACTGGG 1 cut(s) 515
BmsI GCATC 2 cut(s) 833, 1009
BmuI ACTGGG 1 cut(s) 515
BpiI GAAGAC 3 cut(s) 108, 357, 366
BplI GAGNNNNNCTC 2 cut(s) 147, 179
BpmI CTGGAG 3 cut(s) 27, 1408, 1571
BpuEI CTTGAG 1 cut(s) 1157
BsaAI YACGTR 1 cut(s) 26
BsaBI GATNNNNATC 1 cut(s) 618
BsaHI GRCGYC 1 cut(s) 1153
BsaJI CCNNGG 3 cut(s) 955, 1425, 1577
BsaWI WCCGGW 2 cut(s) 134, 756
Bsc4I CCNNNNNNNGG 5 cut(s) 141, 644, 1395, 1452, 1693
Bse118I RCCGGY 1 cut(s) 756
Bse1I ACTGG 3 cut(s) 10, 521, 1554
Bse8I GATNNNNATC 1 cut(s) 618
BseBI CCWGG 3 cut(s) 750, 956, 1426
BseDI CCNNGG 3 cut(s) 955, 1425, 1577
BseGI GGATG 1 cut(s) 1724
BseJI GATNNNNATC 1 cut(s) 618
BseLI CCNNNNNNNGG 5 cut(s) 141, 644, 1395, 1452, 1693
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 3 cut(s) 10, 521, 1554
BseRI GAGGAG 6 cut(s) 335, 465, 468, 471, 1554, 1686
BseXI GCAGC 1 cut(s) 1216
Bsh1236I CGCG 1 cut(s) 318
Bsh1285I CGRYCG 1 cut(s) 493
BshNI GGYRCC 1 cut(s) 1616
BshTI ACCGGT 1 cut(s) 756
BsiEI CGRYCG 1 cut(s) 493
BsiHKAI GWGCWC 1 cut(s) 1557
BsiSI CCGG 2 cut(s) 135, 757
BslI CCNNNNNNNGG 5 cut(s) 141, 644, 1395, 1452, 1693
BsmAI GTCTC 4 cut(s) 917, 954, 1614, 1707
BsmI GAATGC 1 cut(s) 1165
Bsp1286I GDGCHC 1 cut(s) 1557
Bsp143I GATC 5 cut(s) 41, 490, 1120, 1411, 1429
Bsp68I TCGCGA 1 cut(s) 318
BspACI CCGC 4 cut(s) 109, 1000, 1513, 1598
BspCNI CTCAG 1 cut(s) 587
BspFNI CGCG 1 cut(s) 318
BspLI GGNNCC 2 cut(s) 1122, 1618
BspMI ACCTGC 1 cut(s) 1397
BspPI GGATC 4 cut(s) 49, 1115, 1128, 1437
BspT107I GGYRCC 1 cut(s) 1616
BsrFI RCCGGY 1 cut(s) 756
BsrI ACTGG 3 cut(s) 10, 521, 1554
BssAI RCCGGY 1 cut(s) 756
BssECI CCNNGG 3 cut(s) 955, 1425, 1577
BssMI GATC 5 cut(s) 41, 490, 1120, 1411, 1429
BssNI GRCGYC 1 cut(s) 1153
BssT1I CCWWGG 1 cut(s) 1577
Bst2UI CCWGG 3 cut(s) 750, 956, 1426
Bst4CI ACNGT 8 cut(s) 256, 452, 725, 965, 1016, 1194, 1450, 1505
Bst6I CTCTTC 1 cut(s) 915
BstACI GRCGYC 1 cut(s) 1153
BstAPI GCANNNNNTGC 1 cut(s) 1169
BstBAI YACGTR 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 546, 742
BstDEI CTNAG 2 cut(s) 574, 1311
BstEII GGTNACC 1 cut(s) 752
BstF5I GGATG 1 cut(s) 1724
BstFNI CGCG 1 cut(s) 318
BstH2I RGCGCY 1 cut(s) 1687
BstHHI GCGC 1 cut(s) 1686
BstKTI GATC 5 cut(s) 44, 493, 1123, 1414, 1432
BstMAI GTCTC 4 cut(s) 917, 954, 1614, 1707
BstMBI GATC 5 cut(s) 41, 490, 1120, 1411, 1429
BstMCI CGRYCG 1 cut(s) 493
BstMWI GCNNNNNNNGC 4 cut(s) 525, 1054, 1169, 1226
BstNI CCWGG 3 cut(s) 750, 956, 1426
BstNSI RCATGY 1 cut(s) 1561
BstPI GGTNACC 1 cut(s) 752
BstSCI CCNGG 3 cut(s) 748, 954, 1424
BstSFI CTRYAG 2 cut(s) 172, 549
BstSNI TACGTA 1 cut(s) 26
BstUI CGCG 1 cut(s) 318
BstV1I GCAGC 1 cut(s) 1216
BstV2I GAAGAC 3 cut(s) 108, 357, 366
BstX2I RGATCY 1 cut(s) 1120
BstYI RGATCY 1 cut(s) 1120
BtsCI GGATG 1 cut(s) 1724
BtsI GCAGTG 1 cut(s) 44
BtsIMutI CAGTG 7 cut(s) 44, 252, 448, 721, 880, 1021, 1455
BtuMI TCGCGA 1 cut(s) 318
BveI ACCTGC 1 cut(s) 1397
Cac8I GCNNGC 2 cut(s) 546, 742
CfoI GCGC 1 cut(s) 1686
Cfr10I RCCGGY 1 cut(s) 756
Cfr13I GGNCC 2 cut(s) 506, 718
CseI GACGC 1 cut(s) 1142
CsiI ACCWGGT 1 cut(s) 748
Csp6I GTAC 2 cut(s) 27, 805
CspAI ACCGGT 1 cut(s) 756
CviAII CATG 8 cut(s) 355, 368, 516, 712, 809, 1465, 1558, 1613
CviQI GTAC 2 cut(s) 27, 805
DdeI CTNAG 2 cut(s) 574, 1311
DpnI GATC 5 cut(s) 43, 492, 1122, 1413, 1431
DpnII GATC 5 cut(s) 41, 490, 1120, 1411, 1429
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 915
EarI CTCTTC 1 cut(s) 915
EciI GGCGGA 2 cut(s) 1528, 1587
Eco105I TACGTA 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 1577
Eco32I GATATC 1 cut(s) 1653
Eco47I GGWCC 2 cut(s) 506, 718
Eco57I CTGAAG 2 cut(s) 1236, 1359
Eco91I GGTNACC 1 cut(s) 752
EcoO65I GGTNACC 1 cut(s) 752
EcoRII CCWGG 3 cut(s) 748, 954, 1424
EcoRV GATATC 1 cut(s) 1653
EcoT14I CCWWGG 1 cut(s) 1577
ErhI CCWWGG 1 cut(s) 1577
FaeI CATG 8 cut(s) 358, 371, 519, 715, 812, 1468, 1561, 1616
FalI AAGNNNNNCTT 2 cut(s) 771, 803
FatI CATG 8 cut(s) 354, 367, 515, 711, 808, 1464, 1557, 1612
FauI CCCGC 1 cut(s) 116
FauNDI CATATG 1 cut(s) 604
FblI GTMKAC 1 cut(s) 22
Fnu4HI GCNGC 1 cut(s) 1230
FokI GGATG 1 cut(s) 1711
Fsp4HI GCNGC 1 cut(s) 1230
FspBI CTAG 3 cut(s) 555, 627, 1578
GlaI GCGC 1 cut(s) 1685
GluI GCNGC 1 cut(s) 1230
GsuI CTGGAG 3 cut(s) 27, 1408, 1571
HaeII RGCGCY 1 cut(s) 1687
HapII CCGG 2 cut(s) 135, 757
HgaI GACGC 1 cut(s) 1142
HhaI GCGC 1 cut(s) 1686
Hin1I GRCGYC 1 cut(s) 1153
Hin1II CATG 8 cut(s) 358, 371, 519, 715, 812, 1468, 1561, 1616
Hin6I GCGC 1 cut(s) 1684
HinP1I GCGC 1 cut(s) 1684
HindIII AAGCTT 4 cut(s) 930, 1046, 1103, 1438
HinfI GANTC 9 cut(s) 177, 195, 286, 578, 689, 707, 1039, 1461, 1633
HpaII CCGG 2 cut(s) 135, 757
HphI GGTGA 3 cut(s) 437, 573, 746
Hpy166II GTNNAC 4 cut(s) 23, 721, 736, 1667
Hpy188I TCNGA 6 cut(s) 577, 652, 1044, 1079, 1158, 1306
Hpy188III TCNNGA 4 cut(s) 317, 762, 1337, 1642
Hpy8I GTNNAC 4 cut(s) 23, 721, 736, 1667
Hpy99I CGWCG 1 cut(s) 1596
HpyAV CCTTC 4 cut(s) 142, 259, 393, 1334
HpyCH4III ACNGT 8 cut(s) 256, 452, 725, 965, 1016, 1194, 1450, 1505
HpyCH4IV ACGT 2 cut(s) 25, 1005
HpyCH4V TGCA 8 cut(s) 464, 824, 1022, 1057, 1163, 1172, 1294, 1392
HpyF10VI GCNNNNNNNGC 4 cut(s) 525, 1054, 1169, 1226
HpyF3I CTNAG 2 cut(s) 574, 1311
HpySE526I ACGT 2 cut(s) 25, 1005
Hsp92I GRCGYC 1 cut(s) 1153
Hsp92II CATG 8 cut(s) 358, 371, 519, 715, 812, 1468, 1561, 1616
HspAI GCGC 1 cut(s) 1684
Kzo9I GATC 5 cut(s) 41, 490, 1120, 1411, 1429
LmnI GCTCC 3 cut(s) 541, 1482, 1552
Lsp1109I GCAGC 1 cut(s) 1216
LweI GCATC 2 cut(s) 833, 1009
MabI ACCWGGT 1 cut(s) 748
MaeI CTAG 3 cut(s) 555, 627, 1578
MaeII ACGT 2 cut(s) 25, 1005
MaeIII GTNAC 4 cut(s) 196, 340, 752, 1545
MalI GATC 5 cut(s) 43, 492, 1122, 1413, 1431
MboI GATC 5 cut(s) 41, 490, 1120, 1411, 1429
MboII GAAGA 7 cut(s) 108, 362, 371, 932, 952, 1657, 1660
MfeI CAATTG 1 cut(s) 1393
MflI RGATCY 1 cut(s) 1120
MhlI GDGCHC 1 cut(s) 1557
MlyI GAGTC 4 cut(s) 204, 280, 683, 1627
MmeI TCCRAC 3 cut(s) 509, 692, 777
MroXI GAANNNNTTC 1 cut(s) 1365
MseI TTAA 5 cut(s) 375, 1257, 1281, 1436, 1488
MslI CAYNNNNRTG 3 cut(s) 450, 514, 1490
MspA1I CMGCKG 1 cut(s) 830
MspI CCGG 2 cut(s) 135, 757
MspR9I CCNGG 3 cut(s) 750, 956, 1426
MunI CAATTG 1 cut(s) 1393
Mva1269I GAATGC 1 cut(s) 1165
MvaI CCWGG 3 cut(s) 750, 956, 1426
MvnI CGCG 1 cut(s) 318
MwoI GCNNNNNNNGC 4 cut(s) 525, 1054, 1169, 1226
NdeI CATATG 1 cut(s) 604
NdeII GATC 5 cut(s) 41, 490, 1120, 1411, 1429
NlaIII CATG 8 cut(s) 358, 371, 519, 715, 812, 1468, 1561, 1616
NlaIV GGNNCC 2 cut(s) 1122, 1618
NmuCI GTSAC 2 cut(s) 196, 752
NruI TCGCGA 1 cut(s) 318
NspI RCATGY 1 cut(s) 1561
OliI CACNNNNGTG 1 cut(s) 450
PctI GAATGC 1 cut(s) 1165
PdmI GAANNNNTTC 1 cut(s) 1365
PfeI GAWTC 5 cut(s) 177, 578, 707, 1039, 1461
PinAI ACCGGT 1 cut(s) 756
PkrI GCNGC 1 cut(s) 1231
Ple19I CGATCG 1 cut(s) 493
PleI GAGTC 4 cut(s) 203, 280, 683, 1627
PpsI GAGTC 4 cut(s) 203, 280, 683, 1627
Ppu21I YACGTR 1 cut(s) 26
PshBI ATTAAT 2 cut(s) 1257, 1488
Psp1406I AACGTT 1 cut(s) 1005
Psp6I CCWGG 3 cut(s) 748, 954, 1424
PspEI GGTNACC 1 cut(s) 752
PspGI CCWGG 3 cut(s) 748, 954, 1424
PspN4I GGNNCC 2 cut(s) 1122, 1618
PspPI GGNCC 2 cut(s) 506, 718
PsuI RGATCY 1 cut(s) 1120
PvuI CGATCG 1 cut(s) 493
PvuII CAGCTG 1 cut(s) 830
RruI TCGCGA 1 cut(s) 318
RsaI GTAC 2 cut(s) 28, 806
RsaNI GTAC 2 cut(s) 27, 805
RseI CAYNNNNRTG 3 cut(s) 450, 514, 1490
SaqAI TTAA 5 cut(s) 375, 1257, 1281, 1436, 1488
SatI GCNGC 1 cut(s) 1230
Sau3AI GATC 5 cut(s) 41, 490, 1120, 1411, 1429
Sau96I GGNCC 2 cut(s) 506, 718
SchI GAGTC 4 cut(s) 204, 280, 683, 1627
ScrFI CCNGG 3 cut(s) 750, 956, 1426
SduI GDGCHC 1 cut(s) 1557
SexAI ACCWGGT 1 cut(s) 748
SfaNI GCATC 2 cut(s) 833, 1009
SfcI CTRYAG 2 cut(s) 172, 549
SinI GGWCC 2 cut(s) 506, 718
SmiMI CAYNNNNRTG 3 cut(s) 450, 514, 1490
SmlI CTYRAG 1 cut(s) 1136
SmoI CTYRAG 1 cut(s) 1136
SnaBI TACGTA 1 cut(s) 26
SsiI CCGC 4 cut(s) 109, 1000, 1513, 1598
SspMI CTAG 3 cut(s) 555, 627, 1578
StyD4I CCNGG 3 cut(s) 748, 954, 1424
StyI CCWWGG 1 cut(s) 1577
TaaI ACNGT 8 cut(s) 256, 452, 725, 965, 1016, 1194, 1450, 1505
TaiI ACGT 2 cut(s) 28, 1008
TaqI TCGA 3 cut(s) 187, 493, 884
TaqII GACCGA 1 cut(s) 8
TfiI GAWTC 5 cut(s) 177, 578, 707, 1039, 1461
Tru1I TTAA 5 cut(s) 375, 1257, 1281, 1436, 1488
Tru9I TTAA 5 cut(s) 375, 1257, 1281, 1436, 1488
TscAI CASTG 7 cut(s) 51, 259, 455, 728, 880, 1021, 1455
TseFI GTSAC 2 cut(s) 196, 752
TseI GCWGC 1 cut(s) 1229
Tsp45I GTSAC 2 cut(s) 196, 752
TspDTI ATGAA 3 cut(s) 630, 792, 953
TspGWI ACGGA 1 cut(s) 328
TspRI CASTG 7 cut(s) 51, 259, 455, 728, 880, 1021, 1455
VpaK11BI GGWCC 2 cut(s) 506, 718
VspI ATTAAT 2 cut(s) 1257, 1488
XapI RAATTY 4 cut(s) 457, 653, 1378, 1516
XceI RCATGY 1 cut(s) 1561
XmaJI CCTAGG 1 cut(s) 1577
XmiI GTMKAC 1 cut(s) 22
XmnI GAANNNNTTC 1 cut(s) 1365
XspI CTAG 3 cut(s) 555, 627, 1578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.