pycom03g18260

RNA exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0013135
Physical Location & Seq
Reverse (-)
19264591 .. 19267583
2993 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 921 bp
ATGTGTAACCAATATATAAATCGAAAGATATATAAGAAAACGACTAAAAATACGATGTCGTTATTTTTTTTCGCGCATCGCATATTTGTGTTTCCCGGCAGCGGCGGCAGGAGCGGCAGAAGAAAGAAGATGAAAAAGAACCCTAAACAGCAGCAGCAGCTTCAACTGAACCCCAACTGGGCTCAGCTCCAACAGAAGCTCAAGAGTCATGGCTCAACGACAACAACAATCTTAGGGAAGCGGAAAGAGAGACTGGATGTGGAGTTGAATGATGATTCTCCATCTCGTATCAATCCTCTCGATCCAGTTAATGATGATTCCAGCTTGACAGATGAAGTAGCCATGGATTGTGAAATGGTTGGTGTCGGTCAAGGAAACAAAAGCGCCCTTGGACGCGTTACGCTGGTAAATAAATGGGGAAATGTTGTGTATGATGAATTTGTTCGCCCAGTAGAACGTGTTGTTGATTTTCGTACTCAAATAAGTGGCATTCGTCCCCGAGACTTGAGAAAAGCAAAGGATTTTCGAATTGTTCAGAAAAAAGTGGCAGAGTTGATTAAAGGGAGAATTCTTGTGGGCCATGCATTGCGCAATGATCTTAAGGCATTGCTTTTAAGTCATCCCAAGGGGGACATCAGGGATACATTAGAGTATGAACCTTTTATGAGGGGGGAAGGGTCTAGAAGGGCACTTCGGCATCTTGCAGCAGAGTTTCTTGATGTGAAGATCCAAAATGGAGAGCACTGCCCTATTGAAGATGCTCGTGCTGCAATGGTGCTTTATCAGAAAAACAGAAAAGCATGGGAAAAGATAGTTAAAGATCAAATTAAGCTCAAACAAAAGCAGAAAAAACGCAAGCCTAAAAAGAAGCGGATGGGGGGAGATGATTTGGAAACGGACCTTGCAGAACCAGCATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

307

Amino Acids

35.14

Weight (kDa)

10.06

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 113 - 260 9.6e-15 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15080
fragaria_vesca FvH4_3g19101 FvH4_3g19101 FvH4_3g19102
malus_domestica MD03G1236600.v1.1
prunus_persica Prupe.4G172700_v2.0.a1
pyrus_communis pycom03g18260
rosa_chinensis RchiOBHm_Chr5g0031901
rosa_laevigata RLG00000033357
rosa_multiflora Rmu_co8498065.1_g000001
rosa_roxburghii Rroxscaffold_1G00048360
rosa_rugosa Rorug05G0128900 Rorug05G0129000
rosa_samantha Rh5AG223000 Rh5BG223100 Rh5CG246300 Rh5DG228000
rosa_wichuraiana Rw5G020310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 590
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 2 cut(s) 74, 396
AciI CCGC 5 cut(s) 102, 105, 114, 241, 871
AclWI GGATC 2 cut(s) 296, 721
AcsI RAATTY 2 cut(s) 437, 567
AfaI GTAC 1 cut(s) 475
AfiI CCNNNNNNNGG 2 cut(s) 101, 178
AflII CTTAAG 1 cut(s) 599
AflIII ACRYGT 2 cut(s) 394, 457
AgsI TTSAA 3 cut(s) 164, 268, 755
AluBI AGCT 5 cut(s) 160, 187, 199, 324, 832
AluI AGCT 5 cut(s) 160, 187, 199, 324, 832
Alw21I GWGCWC 1 cut(s) 744
Alw26I GTCTC 2 cut(s) 244, 495
AlwI GGATC 2 cut(s) 296, 721
Ama87I CYCGRG 1 cut(s) 498
AoxI GGCC 1 cut(s) 577
ApeKI GCWGC 6 cut(s) 99, 151, 154, 157, 704, 767
ApoI RAATTY 2 cut(s) 437, 567
Asp700I GAANNNNTTC 1 cut(s) 441
AspLEI GCGC 3 cut(s) 76, 386, 591
AspS9I GGNCC 2 cut(s) 577, 898
AsuC2I CCSGG 1 cut(s) 96
AsuII TTCGAA 1 cut(s) 526
AvaI CYCGRG 1 cut(s) 498
AvaII GGWCC 1 cut(s) 898
BaeGI GKGCMC 1 cut(s) 691
BanII GRGCYC 1 cut(s) 184
BauI CACGAG 1 cut(s) 762
Bbv12I GWGCWC 1 cut(s) 744
BbvI GCAGC 6 cut(s) 111, 163, 166, 169, 716, 754
BccI CCATC 2 cut(s) 289, 868
BciVI GTATCC 1 cut(s) 634
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 2 cut(s) 244, 495
BfaI CTAG 2 cut(s) 681, 919
BfoI RGCGCY 1 cut(s) 387
BfrI CTTAAG 1 cut(s) 599
BfuI GTATCC 1 cut(s) 634
BisI GCNGC 9 cut(s) 100, 103, 106, 115, 152, 155, 158, 705, 768
BlpI GCTNAGC 1 cut(s) 183
BlsI GCNGC 9 cut(s) 101, 104, 107, 116, 153, 156, 159, 706, 769
Bme1390I CCNGG 1 cut(s) 96
Bme18I GGWCC 1 cut(s) 898
BmeT110I CYCGRG 1 cut(s) 498
BmgT120I GGNCC 2 cut(s) 577, 898
BmrFI CCNGG 1 cut(s) 96
BmrI ACTGGG 2 cut(s) 187, 443
BmsI GCATC 3 cut(s) 85, 706, 748
BmuI ACTGGG 2 cut(s) 187, 443
Bpu1102I GCTNAGC 1 cut(s) 183
Bpu14I TTCGAA 1 cut(s) 526
BpuEI CTTGAG 2 cut(s) 185, 526
BpuMI CCSGG 1 cut(s) 96
BsaJI CCNNGG 3 cut(s) 342, 388, 624
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 178
Bse1I ACTGG 4 cut(s) 182, 258, 305, 449
Bse3DI GCAATG 4 cut(s) 584, 598, 605, 777
BseDI CCNNGG 3 cut(s) 342, 388, 624
BseGI GGATG 4 cut(s) 262, 619, 879, 914
BseLI CCNNNNNNNGG 2 cut(s) 101, 178
BseMI GCAATG 4 cut(s) 584, 598, 605, 777
BseMII CTCAG 1 cut(s) 197
BseNI ACTGG 4 cut(s) 182, 258, 305, 449
BseSI GKGCMC 1 cut(s) 691
BseXI GCAGC 6 cut(s) 111, 163, 166, 169, 716, 754
Bsh1236I CGCG 2 cut(s) 74, 396
BshFI GGCC 1 cut(s) 579
BsiHKAI GWGCWC 1 cut(s) 744
BsiHKCI CYCGRG 1 cut(s) 498
BsiSI CCGG 1 cut(s) 96
BslFI GGGAC 2 cut(s) 480, 644
BslI CCNNNNNNNGG 2 cut(s) 101, 178
BsmAI GTCTC 2 cut(s) 244, 495
BsmFI GGGAC 2 cut(s) 480, 644
BsmI GAATGC 1 cut(s) 489
BsnI GGCC 1 cut(s) 579
BsoBI CYCGRG 1 cut(s) 498
Bsp119I TTCGAA 1 cut(s) 526
Bsp1286I GDGCHC 3 cut(s) 184, 691, 744
Bsp143I GATC 4 cut(s) 301, 595, 726, 820
Bsp1720I GCTNAGC 1 cut(s) 183
Bsp19I CCATGG 1 cut(s) 342
BspACI CCGC 5 cut(s) 102, 105, 114, 241, 871
BspANI GGCC 1 cut(s) 579
BspCNI CTCAG 1 cut(s) 196
BspFNI CGCG 2 cut(s) 74, 396
BspPI GGATC 2 cut(s) 296, 721
BspT104I TTCGAA 1 cut(s) 526
BspTI CTTAAG 1 cut(s) 599
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 4 cut(s) 584, 598, 605, 777
BsrI ACTGG 4 cut(s) 182, 258, 305, 449
BssECI CCNNGG 3 cut(s) 342, 388, 624
BssMI GATC 4 cut(s) 301, 595, 726, 820
BssSI CACGAG 1 cut(s) 762
BssT1I CCWWGG 3 cut(s) 342, 388, 624
Bst2BI CACGAG 1 cut(s) 762
BstAFI CTTAAG 1 cut(s) 599
BstBI TTCGAA 1 cut(s) 526
BstC8I GCNNGC 1 cut(s) 857
BstDEI CTNAG 2 cut(s) 183, 232
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 4 cut(s) 262, 619, 879, 914
BstFNI CGCG 2 cut(s) 74, 396
BstH2I RGCGCY 1 cut(s) 387
BstHHI GCGC 3 cut(s) 76, 386, 591
BstKTI GATC 4 cut(s) 304, 598, 729, 823
BstMAI GTCTC 2 cut(s) 244, 495
BstMBI GATC 4 cut(s) 301, 595, 726, 820
BstMWI GCNNNNNNNGC 6 cut(s) 105, 111, 114, 157, 767, 911
BstSCI CCNGG 1 cut(s) 94
BstSLI GKGCMC 1 cut(s) 691
BstUI CGCG 2 cut(s) 74, 396
BstV1I GCAGC 6 cut(s) 111, 163, 166, 169, 716, 754
BstX2I RGATCY 1 cut(s) 726
BstYI RGATCY 1 cut(s) 726
BsuI GTATCC 1 cut(s) 634
BsuRI GGCC 1 cut(s) 579
BtgI CCRYGG 1 cut(s) 342
BtgZI GCGATG 1 cut(s) 62
BtsCI GGATG 4 cut(s) 262, 619, 879, 914
BtsI GCAGTG 1 cut(s) 742
BtsIMutI CAGTG 1 cut(s) 742
Cac8I GCNNGC 1 cut(s) 857
CfoI GCGC 3 cut(s) 76, 386, 591
Cfr13I GGNCC 2 cut(s) 577, 898
CseI GACGC 1 cut(s) 402
Csp6I GTAC 1 cut(s) 474
CviAII CATG 4 cut(s) 209, 343, 581, 801
CviQI GTAC 1 cut(s) 474
DdeI CTNAG 2 cut(s) 183, 232
DpnI GATC 4 cut(s) 303, 597, 728, 822
DpnII GATC 4 cut(s) 301, 595, 726, 820
Eco130I CCWWGG 3 cut(s) 342, 388, 624
Eco24I GRGCYC 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 898
Eco88I CYCGRG 1 cut(s) 498
EcoRI GAATTC 1 cut(s) 567
EcoT14I CCWWGG 3 cut(s) 342, 388, 624
EcoT22I ATGCAT 1 cut(s) 586
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 3 cut(s) 342, 388, 624
FaeI CATG 4 cut(s) 212, 346, 584, 804
FaqI GGGAC 2 cut(s) 480, 644
FatI CATG 4 cut(s) 208, 342, 580, 800
Fnu4HI GCNGC 9 cut(s) 100, 103, 106, 115, 152, 155, 158, 705, 768
FokI GGATG 4 cut(s) 269, 606, 886, 901
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 9 cut(s) 100, 103, 106, 115, 152, 155, 158, 705, 768
FspBI CTAG 2 cut(s) 681, 919
FspI TGCGCA 1 cut(s) 590
GlaI GCGC 3 cut(s) 75, 385, 590
GluI GCNGC 9 cut(s) 100, 103, 106, 115, 152, 155, 158, 705, 768
HaeII RGCGCY 1 cut(s) 387
HaeIII GGCC 1 cut(s) 579
HapII CCGG 1 cut(s) 96
HgaI GACGC 1 cut(s) 402
HhaI GCGC 3 cut(s) 76, 386, 591
Hin1II CATG 4 cut(s) 212, 346, 584, 804
Hin6I GCGC 3 cut(s) 74, 384, 589
HinP1I GCGC 3 cut(s) 74, 384, 589
HinfI GANTC 3 cut(s) 205, 275, 317
HpaII CCGG 1 cut(s) 96
Hpy188I TCNGA 2 cut(s) 537, 786
Hpy188III TCNNGA 4 cut(s) 202, 299, 681, 716
HpyAV CCTTC 2 cut(s) 668, 678
HpyCH4IV ACGT 1 cut(s) 457
HpyCH4V TGCA 4 cut(s) 584, 704, 770, 905
HpyF10VI GCNNNNNNNGC 6 cut(s) 105, 111, 114, 157, 767, 911
HpyF3I CTNAG 2 cut(s) 183, 232
HpySE526I ACGT 1 cut(s) 457
Hsp92II CATG 4 cut(s) 212, 346, 584, 804
HspAI GCGC 3 cut(s) 74, 384, 589
Kzo9I GATC 4 cut(s) 301, 595, 726, 820
LmnI GCTCC 2 cut(s) 111, 192
LpnPI CCDG 9 cut(s) 94, 109, 163, 239, 318, 334, 389, 462, 622
Lsp1109I GCAGC 6 cut(s) 111, 163, 166, 169, 716, 754
LweI GCATC 3 cut(s) 85, 706, 748
MaeI CTAG 2 cut(s) 681, 919
MaeII ACGT 1 cut(s) 457
MaeIII GTNAC 2 cut(s) 5, 397
MalI GATC 4 cut(s) 303, 597, 728, 822
MbiI CCGCTC 1 cut(s) 114
MboI GATC 4 cut(s) 301, 595, 726, 820
MboII GAAGA 4 cut(s) 132, 139, 736, 767
MflI RGATCY 1 cut(s) 726
MhlI GDGCHC 3 cut(s) 184, 691, 744
MluCI AATT 4 cut(s) 437, 528, 567, 825
MluI ACGCGT 1 cut(s) 394
MlyI GAGTC 1 cut(s) 214
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 2 cut(s) 306, 660
Mph1103I ATGCAT 1 cut(s) 586
MroXI GAANNNNTTC 1 cut(s) 441
MseI TTAA 6 cut(s) 309, 558, 600, 614, 816, 828
MslI CAYNNNNRTG 1 cut(s) 86
MspA1I CMGCKG 1 cut(s) 102
MspCI CTTAAG 1 cut(s) 599
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 1 cut(s) 96
Mva1269I GAATGC 1 cut(s) 489
MvnI CGCG 2 cut(s) 74, 396
MwoI GCNNNNNNNGC 6 cut(s) 105, 111, 114, 157, 767, 911
NciI CCSGG 1 cut(s) 96
NcoI CCATGG 1 cut(s) 342
NdeII GATC 4 cut(s) 301, 595, 726, 820
NlaIII CATG 4 cut(s) 212, 346, 584, 804
NsbI TGCGCA 1 cut(s) 590
NsiI ATGCAT 1 cut(s) 586
NspV TTCGAA 1 cut(s) 526
PctI GAATGC 1 cut(s) 489
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 2 cut(s) 275, 317
PkrI GCNGC 9 cut(s) 101, 104, 107, 116, 153, 156, 159, 706, 769
PleI GAGTC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 213
PspPI GGNCC 2 cut(s) 577, 898
PsuI RGATCY 1 cut(s) 726
RsaI GTAC 1 cut(s) 475
RsaNI GTAC 1 cut(s) 474
RseI CAYNNNNRTG 1 cut(s) 86
SaqAI TTAA 6 cut(s) 309, 558, 600, 614, 816, 828
SatI GCNGC 9 cut(s) 100, 103, 106, 115, 152, 155, 158, 705, 768
Sau3AI GATC 4 cut(s) 301, 595, 726, 820
Sau96I GGNCC 2 cut(s) 577, 898
SchI GAGTC 1 cut(s) 214
ScrFI CCNGG 1 cut(s) 96
SduI GDGCHC 3 cut(s) 184, 691, 744
SetI ASST 8 cut(s) 162, 189, 201, 326, 460, 661, 834, 903
SfaNI GCATC 3 cut(s) 85, 706, 748
SfuI TTCGAA 1 cut(s) 526
SinI GGWCC 1 cut(s) 898
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 3 cut(s) 200, 505, 599
SmoI CTYRAG 3 cut(s) 200, 505, 599
Sse9I AATT 4 cut(s) 437, 528, 567, 825
SsiI CCGC 5 cut(s) 102, 105, 114, 241, 871
SspMI CTAG 2 cut(s) 681, 919
StyD4I CCNGG 1 cut(s) 94
StyI CCWWGG 3 cut(s) 342, 388, 624
TaiI ACGT 1 cut(s) 460
TaqI TCGA 3 cut(s) 22, 300, 526
TaqII GACCGA 1 cut(s) 356
TasI AATT 4 cut(s) 437, 528, 567, 825
TauI GCSGC 3 cut(s) 105, 108, 117
TfiI GAWTC 2 cut(s) 275, 317
Tru1I TTAA 6 cut(s) 309, 558, 600, 614, 816, 828
Tru9I TTAA 6 cut(s) 309, 558, 600, 614, 816, 828
TscAI CASTG 1 cut(s) 749
TseI GCWGC 6 cut(s) 99, 151, 154, 157, 704, 767
TspDTI ATGAA 4 cut(s) 146, 348, 450, 669
TspGWI ACGGA 1 cut(s) 911
TspRI CASTG 1 cut(s) 749
Vha464I CTTAAG 1 cut(s) 599
VpaK11BI GGWCC 1 cut(s) 898
XapI RAATTY 2 cut(s) 437, 567
XbaI TCTAGA 1 cut(s) 680
XmnI GAANNNNTTC 1 cut(s) 441
XspI CTAG 2 cut(s) 681, 919
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.