pycom03g19420
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0018704
Physical Location & Seq
Forward (+)
20018313 .. 20018639
327 bp
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UTR
Exon/CDS
Intron
pycom03g19420.1

Sequence Viewer

Length: 327 bp
ATGTGTGAAAATTGGTCAAATCTCTGTATGGCCTCAAGCAAGCACCTCGTGCTTGAAATTCAAAATTCACATCATATTTGCCATCTTTACATTTTTCTTGTCTTAGATTTTGATACTAGTCTGTTTGTCAAATATGTGAATAGACATGTGGTTATTATGCTACTATATGTTGATGATATAATCATCACTGGATCTGATACTGTGTTAATTCAACAAGTCATTGATGAATTGGGTGCTGTGTTTGAAATGAAAGACATGGGCAAACTCACTTTCTTCCTCAGGCTGCAGATTACCTACAAAGATAATGATGACCTGTTTATTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

109

Amino Acids

12.6

Weight (kDa)

4.89

Isoelectric Point (pI)

25.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 36 - 99 2.7e-11 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018704)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03940
malus_domestica MD17G1120000.v1.1
pyrus_communis pycom03g19420 pycom10g09460 pycom10g21720 pycom13g28160 pycom14g05170
rosa_chinensis RchiOBHm_Chr3g0491631
rosa_wichuraiana Rw5G011130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 199
AcsI RAATTY 2 cut(s) 57, 64
AdeI CACNNNGTG 1 cut(s) 49
AflIII ACRYGT 1 cut(s) 145
AgsI TTSAA 4 cut(s) 56, 62, 212, 245
AhlI ACTAGT 1 cut(s) 116
AlwI GGATC 1 cut(s) 199
AoxI GGCC 1 cut(s) 30
ApeKI GCWGC 1 cut(s) 283
ApoI RAATTY 2 cut(s) 57, 64
AxyI CCTNAGG 1 cut(s) 278
BauI CACGAG 1 cut(s) 47
BbvI GCAGC 1 cut(s) 270
BccI CCATC 1 cut(s) 90
BcgI CGANNNNNNTGC 2 cut(s) 28, 62
BcuI ACTAGT 1 cut(s) 116
BfaI CTAG 1 cut(s) 117
BfmI CTRYAG 1 cut(s) 284
BisI GCNGC 1 cut(s) 284
BlsI GCNGC 1 cut(s) 285
BpuEI CTTGAG 1 cut(s) 19
Bse1I ACTGG 1 cut(s) 193
Bse21I CCTNAGG 1 cut(s) 278
BseMII CTCAG 1 cut(s) 292
BseNI ACTGG 1 cut(s) 193
BseXI GCAGC 1 cut(s) 270
BshFI GGCC 1 cut(s) 32
BsnI GGCC 1 cut(s) 32
Bsp143I GATC 1 cut(s) 191
BspANI GGCC 1 cut(s) 32
BspCNI CTCAG 1 cut(s) 291
BspMAI CTGCAG 1 cut(s) 288
BspPI GGATC 1 cut(s) 199
BsrI ACTGG 1 cut(s) 193
BssMI GATC 1 cut(s) 191
BssSI CACGAG 1 cut(s) 47
Bst2BI CACGAG 1 cut(s) 47
Bst4CI ACNGT 1 cut(s) 202
BstAPI GCANNNNNTGC 1 cut(s) 49
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 2 cut(s) 103, 278
BstKTI GATC 1 cut(s) 194
BstMBI GATC 1 cut(s) 191
BstMWI GCNNNNNNNGC 1 cut(s) 49
BstNSI RCATGY 1 cut(s) 149
BstSFI CTRYAG 1 cut(s) 284
BstV1I GCAGC 1 cut(s) 270
BstX2I RGATCY 1 cut(s) 191
BstYI RGATCY 1 cut(s) 191
Bsu36I CCTNAGG 1 cut(s) 278
BsuRI GGCC 1 cut(s) 32
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 41
CviAII CATG 2 cut(s) 146, 256
CviJI RGCY 2 cut(s) 32, 283
CviKI_1 RGCY 2 cut(s) 32, 283
DdeI CTNAG 2 cut(s) 103, 278
DpnI GATC 1 cut(s) 193
DpnII GATC 1 cut(s) 191
DraIII CACNNNGTG 1 cut(s) 49
Eco81I CCTNAGG 1 cut(s) 278
FaeI CATG 2 cut(s) 149, 259
FatI CATG 2 cut(s) 145, 255
Fnu4HI GCNGC 1 cut(s) 284
Fsp4HI GCNGC 1 cut(s) 284
FspBI CTAG 1 cut(s) 117
GluI GCNGC 1 cut(s) 284
HaeIII GGCC 1 cut(s) 32
Hin1II CATG 2 cut(s) 149, 259
Hpy188I TCNGA 1 cut(s) 196
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4V TGCA 1 cut(s) 286
HpyF10VI GCNNNNNNNGC 1 cut(s) 49
HpyF3I CTNAG 2 cut(s) 103, 278
Hsp92II CATG 2 cut(s) 149, 259
Kzo9I GATC 1 cut(s) 191
LpnPI CCDG 2 cut(s) 174, 265
Lsp1109I GCAGC 1 cut(s) 270
MaeI CTAG 1 cut(s) 117
MalI GATC 1 cut(s) 193
MboI GATC 1 cut(s) 191
MboII GAAGA 1 cut(s) 265
MflI RGATCY 1 cut(s) 191
MluCI AATT 5 cut(s) 10, 57, 64, 207, 227
MnlI CCTC 3 cut(s) 43, 56, 287
MseI TTAA 1 cut(s) 206
MwoI GCNNNNNNNGC 1 cut(s) 49
NdeII GATC 1 cut(s) 191
NlaIII CATG 2 cut(s) 149, 259
NspI RCATGY 1 cut(s) 149
PciI ACATGT 1 cut(s) 145
PkrI GCNGC 1 cut(s) 285
PscI ACATGT 1 cut(s) 145
PstI CTGCAG 1 cut(s) 288
PsuI RGATCY 1 cut(s) 191
SaqAI TTAA 1 cut(s) 206
SatI GCNGC 1 cut(s) 284
Sau3AI GATC 1 cut(s) 191
SetI ASST 3 cut(s) 48, 296, 315
SfcI CTRYAG 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
SpeI ACTAGT 1 cut(s) 116
Sse9I AATT 5 cut(s) 10, 57, 64, 207, 227
SspMI CTAG 1 cut(s) 117
TaaI ACNGT 1 cut(s) 202
TasI AATT 5 cut(s) 10, 57, 64, 207, 227
Tru1I TTAA 1 cut(s) 206
Tru9I TTAA 1 cut(s) 206
TscAI CASTG 1 cut(s) 193
TseI GCWGC 1 cut(s) 283
TspDTI ATGAA 3 cut(s) 240, 263, 312
TspRI CASTG 1 cut(s) 193
XapI RAATTY 2 cut(s) 57, 64
XceI RCATGY 1 cut(s) 149
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.