pycom03g23290
ERF Family

Calcium-dependent phosphotriesterase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0013143
Physical Location & Seq
Reverse (-)
23165044 .. 23167697
2654 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g23290.1

Sequence Viewer

Length: 987 bp
ATGGCTTACTGCTCCTCCCTCTGCTCCCCCAAATTCCTCCTCCTCTTCCTCCTCCTCTCCGCCGTCCCCATCGCCTACCTCATCTCCCTCGAGGTCGCCAAACCCGCCACCCACGTCTACCACTACCACAGCACCAGCTGGTTCCGCGAGTGCGCCAAGTGGGACGATCTCAACCGTCGCTTCCTTGTCTCCTTCATAGACGGCGGAATCGGCCAGGTAACGGTGCCCAGCGACGATTCCGATGCGGTTCTGGTGGAAGTCCCCGTGGTCAAACACGTTGACTTGGCCGGAAATGCTTCGCTCGGACTCGTCATCGACCGGCCCAGGAATCGCCTCCTCGTCGCCACTGCTGACGTCAAGGGCAACTTATATAGTGCGCTGGCGGCCTACGACTTGTCCACGTGGGAACGGGTGTTTCTCACTCAACTCAGCGGCCCAAGTGATGAGAAATCATTCGCAGATGATGTTGCAGTTGATGCAGAAGGTAATGCATATGTGACAGATTGCACAGCCAGTAAAATTTGGAAGGTTGGAGCTGACGGGAAGCTCCTCTCTACAATTAGAAACCCTCTCTTCAAAGCAAAGGAGTGGTACAAAAACTTGGTTGGCCTAAACGGAATCGTTTACCACCCAGATGGCTACTTGATAGTCATTCACACTTTCAGTGGCAAATTGTTGAAGATTGATCTAGCAAAGGGAGAGGAAGTGAAGCTCATTGAGGTAGCTGGAGGACCGCTAGCATTTGGGGATGGTCTAGAGCTCCTATCTCCCACCAAGGTTGTAGTTGCCGGCAACCCTTCTGGTAGATTAGTGGAGAGCTCTGACGGGTGGGAGACAGCTTCCGTTGTGGGAAAATTCTCAGGCCCAAAGCATCGGTTGGCCACAGCAGCAACCGTGAAGGACGGGAAGGTGTACCTCAGCCACATGGTTGGCATGGGATACCCCAAGAAGACGCATGCCCTCGTTGAGGCAGTATTTTCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SGL

Protein Analysis

329

Amino Acids

35.41

Weight (kDa)

6.5

Isoelectric Point (pI)

18.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G16760 AT2G47370 AT2G47370 AT5G28660
fragaria_vesca FvH4_5g39130
malus_domestica MD03G1294500.v1.1
prunus_persica Prupe.8G266600_v2.0.a1
pyrus_communis pycom03g23290
rosa_chinensis RchiOBHm_Chr7g0241121
rosa_laevigata RLG00000000595
rosa_multiflora Rmu_sc0008150.1_g000002
rosa_roxburghii Rroxscaffold_3G00219250
rosa_rugosa Rorug07G0342600
rosa_samantha Rh7AG497700 Rh7BG468500 Rh7CG514600 Rh7DG481900
rosa_wichuraiana Rw7G041990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 357
AbsI CCTCGAGG 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 223
AccI GTMKAC 1 cut(s) 117
AccII CGCG 1 cut(s) 147
AciI CCGC 8 cut(s) 60, 105, 145, 204, 245, 383, 432, 734
AcoI YGGCCR 3 cut(s) 211, 285, 879
AcsI RAATTY 3 cut(s) 32, 519, 854
AcvI CACGTG 1 cut(s) 402
AcyI GRCGYC 1 cut(s) 354
AfaI GTAC 2 cut(s) 593, 914
AfiI CCNNNNNNNGG 2 cut(s) 220, 967
AflIII ACRYGT 1 cut(s) 274
AgsI TTSAA 2 cut(s) 577, 679
AjiI CACGTC 1 cut(s) 115
AjnI CCWGG 2 cut(s) 213, 323
AluBI AGCT 8 cut(s) 138, 536, 547, 712, 725, 760, 819, 839
AluI AGCT 8 cut(s) 138, 536, 547, 712, 725, 760, 819, 839
Alw21I GWGCWC 2 cut(s) 762, 821
Alw26I GTCTC 2 cut(s) 193, 827
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 8 cut(s) 211, 285, 320, 384, 433, 607, 862, 879
ApeKI GCWGC 1 cut(s) 887
ApoI RAATTY 3 cut(s) 32, 519, 854
Asp700I GAANNNNTTC 2 cut(s) 295, 452
AspLEI GCGC 2 cut(s) 155, 379
AspS9I GGNCC 4 cut(s) 321, 434, 731, 863
AsuNHI GCTAGC 1 cut(s) 736
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 1 cut(s) 731
BaeGI GKGCMC 1 cut(s) 228
BalI TGGCCA 1 cut(s) 881
BanI GGYRCC 1 cut(s) 223
BanII GRGCYC 2 cut(s) 762, 821
BbrPI CACGTG 1 cut(s) 402
BbsI GAAGAC 1 cut(s) 956
Bbv12I GWGCWC 2 cut(s) 762, 821
BbvCI CCTCAGC 1 cut(s) 917
BbvI GCAGC 1 cut(s) 899
BccI CCATC 3 cut(s) 77, 629, 743
BceAI ACGGC 2 cut(s) 47, 217
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BciT130I CCWGG 2 cut(s) 215, 325
BciVI GTATCC 1 cut(s) 932
BcoDI GTCTC 2 cut(s) 193, 827
BfaI CTAG 3 cut(s) 689, 737, 755
BfuI GTATCC 1 cut(s) 932
BisI GCNGC 3 cut(s) 384, 433, 888
BlsI GCNGC 3 cut(s) 385, 434, 889
Bme1390I CCNGG 2 cut(s) 215, 325
Bme18I GGWCC 1 cut(s) 731
BmeT110I CYCGRG 1 cut(s) 89
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 4 cut(s) 321, 434, 731, 863
BmiI GGNNCC 2 cut(s) 143, 225
BmrFI CCNGG 2 cut(s) 215, 325
BmsI GCATC 3 cut(s) 232, 466, 880
BmtI GCTAGC 1 cut(s) 740
BpiI GAAGAC 1 cut(s) 956
BpmI CTGGAG 1 cut(s) 747
Bpu10I CCTNAGC 1 cut(s) 917
BsaAI YACGTR 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 354
BsaJI CCNNGG 3 cut(s) 264, 323, 774
BsaXI ACNNNNNCTCC 5 cut(s) 29, 68, 98, 690, 720
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 967
Bse118I RCCGGY 2 cut(s) 318, 788
Bse1I ACTGG 1 cut(s) 513
BseBI CCWGG 2 cut(s) 215, 325
BseDI CCNNGG 3 cut(s) 264, 323, 774
BseGI GGATG 1 cut(s) 754
BseLI CCNNNNNNNGG 2 cut(s) 220, 967
BseMII CTCAG 3 cut(s) 442, 873, 931
BseNI ACTGG 1 cut(s) 513
BseRI GAGGAG 7 cut(s) 4, 29, 32, 41, 44, 326, 539
BseSI GKGCMC 1 cut(s) 228
BseXI GCAGC 1 cut(s) 899
BseYI CCCAGC 1 cut(s) 227
Bsh1236I CGCG 1 cut(s) 147
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 8 cut(s) 213, 287, 322, 386, 435, 609, 864, 881
BshNI GGYRCC 1 cut(s) 223
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 2 cut(s) 762, 821
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 3 cut(s) 288, 319, 789
BslFI GGGAC 3 cut(s) 50, 176, 245
BslI CCNNNNNNNGG 2 cut(s) 220, 967
BsmAI GTCTC 2 cut(s) 193, 827
BsmFI GGGAC 3 cut(s) 50, 176, 245
BsnI GGCC 8 cut(s) 213, 287, 322, 386, 435, 609, 864, 881
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 3 cut(s) 228, 762, 821
Bsp143I GATC 2 cut(s) 166, 685
BspACI CCGC 8 cut(s) 60, 105, 145, 204, 245, 383, 432, 734
BspANI GGCC 8 cut(s) 213, 287, 322, 386, 435, 609, 864, 881
BspCNI CTCAG 3 cut(s) 441, 872, 930
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 2 cut(s) 143, 225
BspOI GCTAGC 1 cut(s) 740
BspT107I GGYRCC 1 cut(s) 223
BsrFI RCCGGY 2 cut(s) 318, 788
BsrI ACTGG 1 cut(s) 513
BssAI RCCGGY 2 cut(s) 318, 788
BssECI CCNNGG 3 cut(s) 264, 323, 774
BssMI GATC 2 cut(s) 166, 685
BssNI GRCGYC 1 cut(s) 354
BssT1I CCWWGG 1 cut(s) 774
Bst2UI CCWGG 2 cut(s) 215, 325
Bst4CI ACNGT 3 cut(s) 176, 223, 895
Bst6I CTCTTC 2 cut(s) 50, 578
BstACI GRCGYC 1 cut(s) 354
BstAPI GCANNNNNTGC 1 cut(s) 476
BstBAI YACGTR 1 cut(s) 402
BstC8I GCNNGC 4 cut(s) 381, 738, 790, 957
BstDEI CTNAG 3 cut(s) 428, 859, 917
BstDSI CCRYGG 1 cut(s) 264
BstENI CCTNNNNNAGG 1 cut(s) 965
BstF5I GGATG 1 cut(s) 754
BstFNI CGCG 1 cut(s) 147
BstHHI GCGC 2 cut(s) 155, 379
BstKTI GATC 2 cut(s) 169, 688
BstMAI GTCTC 2 cut(s) 193, 827
BstMBI GATC 2 cut(s) 166, 685
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 7 cut(s) 104, 144, 210, 293, 383, 476, 887
BstNI CCWGG 2 cut(s) 215, 325
BstNSI RCATGY 1 cut(s) 959
BstSCI CCNGG 2 cut(s) 213, 323
BstSLI GKGCMC 1 cut(s) 228
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 1 cut(s) 899
BstV2I GAAGAC 1 cut(s) 956
BstXI CCANNNNNNTGG 2 cut(s) 635, 929
BsuI GTATCC 1 cut(s) 932
BsuRI GGCC 8 cut(s) 213, 287, 322, 386, 435, 609, 864, 881
BtgI CCRYGG 1 cut(s) 264
BtgZI GCGATG 1 cut(s) 55
BtrI CACGTC 1 cut(s) 115
BtsCI GGATG 1 cut(s) 754
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 2 cut(s) 345, 670
Cac8I GCNNGC 4 cut(s) 381, 738, 790, 957
CfoI GCGC 2 cut(s) 155, 379
Cfr10I RCCGGY 2 cut(s) 318, 788
Cfr13I GGNCC 4 cut(s) 321, 434, 731, 863
CseI GACGC 1 cut(s) 961
Csp6I GTAC 2 cut(s) 592, 913
CspCI CAANNNNNGTGG 2 cut(s) 760, 795
CviAII CATG 3 cut(s) 925, 934, 956
CviQI GTAC 2 cut(s) 592, 913
DdeI CTNAG 3 cut(s) 428, 859, 917
DpnI GATC 2 cut(s) 168, 687
DpnII GATC 2 cut(s) 166, 685
EaeI YGGCCR 3 cut(s) 211, 285, 879
Eam1104I CTCTTC 2 cut(s) 50, 578
EarI CTCTTC 2 cut(s) 50, 578
EciI GGCGGA 2 cut(s) 49, 219
Ecl136II GAGCTC 2 cut(s) 760, 819
Eco130I CCWWGG 1 cut(s) 774
Eco24I GRGCYC 2 cut(s) 762, 821
Eco47I GGWCC 1 cut(s) 731
Eco53kI GAGCTC 2 cut(s) 760, 819
Eco72I CACGTG 1 cut(s) 402
Eco88I CYCGRG 1 cut(s) 89
EcoICRI GAGCTC 2 cut(s) 760, 819
EcoNI CCTNNNNNAGG 1 cut(s) 965
EcoRII CCWGG 2 cut(s) 213, 323
EcoT14I CCWWGG 1 cut(s) 774
EcoT22I ATGCAT 1 cut(s) 493
EcoT38I GRGCYC 2 cut(s) 762, 821
ErhI CCWWGG 1 cut(s) 774
FaeI CATG 3 cut(s) 928, 937, 959
FaiI YATR 8 cut(s) 197, 370, 372, 493, 495, 926, 935, 957
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FaqI GGGAC 3 cut(s) 50, 176, 245
FatI CATG 3 cut(s) 924, 933, 955
FauI CCCGC 1 cut(s) 112
FauNDI CATATG 1 cut(s) 493
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 3 cut(s) 384, 433, 888
FokI GGATG 1 cut(s) 761
FriOI GRGCYC 2 cut(s) 762, 821
Fsp4HI GCNGC 3 cut(s) 384, 433, 888
FspBI CTAG 3 cut(s) 689, 737, 755
GlaI GCGC 2 cut(s) 154, 378
GluI GCNGC 3 cut(s) 384, 433, 888
GsaI CCCAGC 1 cut(s) 231
GsuI CTGGAG 1 cut(s) 747
HaeIII GGCC 8 cut(s) 213, 287, 322, 386, 435, 609, 864, 881
HapII CCGG 3 cut(s) 288, 319, 789
HgaI GACGC 1 cut(s) 961
HhaI GCGC 2 cut(s) 155, 379
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 3 cut(s) 928, 937, 959
Hin6I GCGC 2 cut(s) 153, 377
HinP1I GCGC 2 cut(s) 153, 377
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 5 cut(s) 207, 236, 306, 328, 618
HpaII CCGG 3 cut(s) 288, 319, 789
Hpy166II GTNNAC 5 cut(s) 118, 280, 399, 625, 913
Hpy188I TCNGA 3 cut(s) 241, 305, 823
Hpy188III TCNNGA 1 cut(s) 755
Hpy8I GTNNAC 5 cut(s) 118, 280, 399, 625, 913
Hpy99I CGWCG 3 cut(s) 180, 236, 344
HpyAV CCTTC 6 cut(s) 202, 476, 520, 807, 892, 901
HpyCH4III ACNGT 3 cut(s) 176, 223, 895
HpyCH4IV ACGT 4 cut(s) 114, 276, 354, 401
HpyCH4V TGCA 4 cut(s) 470, 479, 491, 507
HpyF10VI GCNNNNNNNGC 7 cut(s) 104, 144, 210, 293, 383, 476, 887
HpyF3I CTNAG 3 cut(s) 428, 859, 917
HpySE526I ACGT 4 cut(s) 114, 276, 354, 401
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 3 cut(s) 928, 937, 959
HspAI GCGC 2 cut(s) 153, 377
KroI GCCGGC 1 cut(s) 788
KroNI GCCGGC 1 cut(s) 790
Kzo9I GATC 2 cut(s) 166, 685
LmnI GCTCC 5 cut(s) 17, 29, 533, 552, 765
Lsp1109I GCAGC 1 cut(s) 899
LweI GCATC 3 cut(s) 232, 466, 880
MaeI CTAG 3 cut(s) 689, 737, 755
MaeII ACGT 4 cut(s) 114, 276, 354, 401
MaeIII GTNAC 2 cut(s) 217, 496
MalI GATC 2 cut(s) 168, 687
MboI GATC 2 cut(s) 166, 685
MboII GAAGA 4 cut(s) 37, 565, 691, 961
MhlI GDGCHC 3 cut(s) 228, 762, 821
MlsI TGGCCA 1 cut(s) 881
MluCI AATT 5 cut(s) 32, 519, 558, 671, 854
MluNI TGGCCA 1 cut(s) 881
MlyI GAGTC 1 cut(s) 300
MmeI TCCRAC 1 cut(s) 511
Mox20I TGGCCA 1 cut(s) 881
Mph1103I ATGCAT 1 cut(s) 493
MroNI GCCGGC 1 cut(s) 788
MroXI GAANNNNTTC 2 cut(s) 295, 452
MscI TGGCCA 1 cut(s) 881
MslI CAYNNNNRTG 1 cut(s) 633
Msp20I TGGCCA 1 cut(s) 881
MspA1I CMGCKG 2 cut(s) 138, 432
MspI CCGG 3 cut(s) 288, 319, 789
MspR9I CCNGG 2 cut(s) 215, 325
MvaI CCWGG 2 cut(s) 215, 325
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 7 cut(s) 104, 144, 210, 293, 383, 476, 887
NaeI GCCGGC 1 cut(s) 790
NdeI CATATG 1 cut(s) 493
NdeII GATC 2 cut(s) 166, 685
NgoMIV GCCGGC 1 cut(s) 788
NheI GCTAGC 1 cut(s) 736
NlaIII CATG 3 cut(s) 928, 937, 959
NlaIV GGNNCC 2 cut(s) 143, 225
NmuCI GTSAC 1 cut(s) 496
NsiI ATGCAT 1 cut(s) 493
NspI RCATGY 1 cut(s) 959
PaeI GCATGC 1 cut(s) 959
PaeR7I CTCGAG 1 cut(s) 89
PdiI GCCGGC 1 cut(s) 790
PdmI GAANNNNTTC 2 cut(s) 295, 452
PfeI GAWTC 4 cut(s) 207, 236, 328, 618
PkrI GCNGC 3 cut(s) 385, 434, 889
PleI GAGTC 1 cut(s) 300
PmaCI CACGTG 1 cut(s) 402
PmlI CACGTG 1 cut(s) 402
PpsI GAGTC 1 cut(s) 300
Ppu21I YACGTR 1 cut(s) 402
Psp124BI GAGCTC 2 cut(s) 762, 821
Psp6I CCWGG 2 cut(s) 213, 323
PspCI CACGTG 1 cut(s) 402
PspFI CCCAGC 1 cut(s) 227
PspGI CCWGG 2 cut(s) 213, 323
PspN4I GGNNCC 2 cut(s) 143, 225
PspPI GGNCC 4 cut(s) 321, 434, 731, 863
PspXI VCTCGAGB 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 138
RsaI GTAC 2 cut(s) 593, 914
RsaNI GTAC 2 cut(s) 592, 913
RseI CAYNNNNRTG 1 cut(s) 633
SacI GAGCTC 2 cut(s) 762, 821
SatI GCNGC 3 cut(s) 384, 433, 888
Sau3AI GATC 2 cut(s) 166, 685
Sau96I GGNCC 4 cut(s) 321, 434, 731, 863
SchI GAGTC 1 cut(s) 300
ScrFI CCNGG 2 cut(s) 215, 325
SduI GDGCHC 3 cut(s) 228, 762, 821
SfaNI GCATC 3 cut(s) 232, 466, 880
Sfr274I CTCGAG 1 cut(s) 89
SinI GGWCC 1 cut(s) 731
SlaI CTCGAG 1 cut(s) 89
SmiMI CAYNNNNRTG 1 cut(s) 633
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
SphI GCATGC 1 cut(s) 959
Sse9I AATT 5 cut(s) 32, 519, 558, 671, 854
SsiI CCGC 8 cut(s) 60, 105, 145, 204, 245, 383, 432, 734
SspMI CTAG 3 cut(s) 689, 737, 755
SstI GAGCTC 2 cut(s) 762, 821
StyD4I CCNGG 2 cut(s) 213, 323
StyI CCWWGG 1 cut(s) 774
TaaI ACNGT 3 cut(s) 176, 223, 895
TaiI ACGT 4 cut(s) 117, 279, 357, 404
TaqI TCGA 2 cut(s) 90, 315
TasI AATT 5 cut(s) 32, 519, 558, 671, 854
TauI GCSGC 2 cut(s) 386, 435
TfiI GAWTC 4 cut(s) 207, 236, 328, 618
TscAI CASTG 2 cut(s) 352, 670
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 1 cut(s) 887
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 1 cut(s) 184
TspGWI ACGGA 2 cut(s) 630, 832
TspRI CASTG 2 cut(s) 352, 670
VpaK11BI GGWCC 1 cut(s) 731
XagI CCTNNNNNAGG 1 cut(s) 965
XapI RAATTY 3 cut(s) 32, 519, 854
XbaI TCTAGA 1 cut(s) 754
XceI RCATGY 1 cut(s) 959
XhoI CTCGAG 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 117
XmnI GAANNNNTTC 2 cut(s) 295, 452
XspI CTAG 3 cut(s) 689, 737, 755
ZraI GACGTC 1 cut(s) 355
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.