Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0015256

Physical Location & Seq

Genomic Coordinates Forward (+)

173414 .. 175234

Gene Structure 1821 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g00220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1821 bp

ATGCATCGCCATGTGACTGTGATCAGCCGGTTCCCGTCAATTCCAAGCCCCGCAGACACGATCTCAGCCCTTAACTTTAAAATCAAAGACTTGGTTTCCAAGGGATTATATGACCAAACCCTTCATCTATACAAACTGCAACTCCATCCCCATGGCCTTCATGCAAACAACTCCATTCTTCCTTCAATCATTAAAGCTTGCTCCTACGCAATGTCCCGCCACTTCGGCCTTCAACTTCACTGCGTCGCTCTCAAGTCGGGCTCCGACTCAGACCCGGTCGTATCCAATTCTCTCATCTCCATGTACGCCAAATTTACAACTGTTGAAGCAGCACGTCAGGTGTTTGATGAAATGCCTGAAAGAGACACCATCACCTGGAATTCCATGATAAATTGCTATTTGCAAAATGGGCATCTTGAAAAAGGTTTGCAAGTGCTTAAGCAAATGCATTTTAATGGTTTTGTACCGAAAGCAGAGTTGATTGCTGGCATAATGTCAGTTTGCGCGAGGTCAGGAGAGTTGAGACGACTGGGCAGAGAGATTCACGGGCTCGTTATTGTTCATGGAATGATTAAGGAGTCGGTGTTCTTGTCGACGGCTCTGGTTGATTTGTATATGAGGTTTCGCGACTTGTCGATGGCTTTACATGTATTTTATCATATGGAAGTTAAAAATGAAGTTTCTTGGACTGCCTTAGTTTCGGGATGCATTGCTAATCACAATTATGGCATGGCCCTGGGTTGCTTCCGGGCAATGCAGGTTGAAGGCCTGAAACCCAACAGAGTGACTGTGCTTGCCATTTTACCAGCTTGTGCCGAGTTGGGACTTGTAGAACATGGGAAAGAGATTCATGCATATATCCTTCATCATGGGTTTGATTCCAATAATCATTGCTCAGCAGCTCTCATACACATGTATTGCAAATGTGGGAAAGTTTTGCGTTCTGCCAAGTTAATTTTCGATAGGGAAGCTGTTAAAGATGTTGTGATATGGAGCTCAATTATTGGGAGCTATTCTCAATGCAGGGATTACACGAAATCGCTGAAACTCTTCAGCCAAATGCGAGCTGAAGGAATTGAACCCAACTCTGTCACTGTATTGGCAATCATTTCTGTCTGCGCTTCCCTGTCTTCACTAATCCTTGGCTGTGGAGTCCATGGCTATGTCTTAAAATCTGGGTTAAGTTTTGACACTTTCATTGGGAATGCACTCATCAATATGTATGCAAAGTGTGGTTGTCTCAAGGATTCACATCAGGTTTTCAAGGAGATGCCGAGCAAAGATTCTGTTTCATGGAGCGCACTGATTGGCAGTTATGGCCTTCATGGGGATTCCGAGAAAGCTCTGCAACTCTTTGATGAGATGCAAGAGAGAGGGGTGGAGCCTGATGCAGTTACTTTCCTCTCTGTTTTATCTGCTTGCAACCACGCCGGCCTAGTGGAAGAAGGGAATAAGGTCTTTAAGCGTCTGACAGAAGGTAATAAAATAGAATTAAGCGTGGAGCACTACGCTTGCTTTATCGATCTTCTTGGAAGGACGGGCAAGCTTGAAGATGCTTATGAGCTAGTGAGATCAATGCCCATGACACCAAGTACTAGAATTTGGACTTCTTTGGTTACAGCTTGCAAGATTCATGGAAGATTAGACAAGGCAGAATTTTTAGCACATGAGCTTGTTACCCTGGAGCCTGAGAATGCTGCCCACTATACCTTACTGAGCACGGTTCATGCTGAAACCGGAAACTGGCTTGGCGTGGAAGAGGTGAGGAGAGCCATGAGAATACAAGGATTAAGGAAAGGCTACGGATTCAGCCGAATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

607

Amino Acids

67.32

Weight (kDa)

8.04

Isoelectric Point (pI)

40.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	121 - 156	4.1e-07	PPR repeat family
PPR	PF01535	124 - 154	1.6e-07	PPR repeat
PPR_2	PF13041	225 - 272	3.1e-06	PPR repeat family
PPR_2	PF13041	326 - 374	2.6e-09	PPR repeat family
PPR_2	PF13041	427 - 474	1.2e-12	PPR repeat family
PPR	PF01535	430 - 460	9.3e-09	PPR repeat
E_motif	PF20431	548 - 606	5.2e-10	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015256)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31070 AT4G31070
fragaria_vesca	FvH4_4g00140
malus_domestica	MD04G1001600.v1.1
prunus_persica	Prupe.1G001600_v2.0.a1
pyrus_communis	pycom04g00220
rosa_chinensis	RchiOBHm_Chr4g0384761
rosa_laevigata	RLG00000010274
rosa_roxburghii	Rroxscaffold_5G00332820
rosa_rugosa	Rorug03G0297500
rosa_samantha	Rh4AG001000 Rh4BG000900 Rh4CG000800 Rh4DG000900
rosa_wichuraiana	Rw0G021980 Rw4G000790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	748
AccB7I	CCANNNNNTGG	1 cut(s)	375
AccI	GTMKAC	1 cut(s)	593
AccII	CGCG	2 cut(s)	506, 627
AciI	CCGC	2 cut(s)	51, 217
AcsI	RAATTY	5 cut(s)	311, 379, 1599, 1655, 1815
AcuI	CTGAAG	2 cut(s)	1036, 1089
AfaI	GTAC	3 cut(s)	305, 465, 1594
AfiI	CCNNNNNNNGG	3 cut(s)	375, 1327, 1743
AflII	CTTAAG	1 cut(s)	437
AflIII	ACRYGT	2 cut(s)	646, 912
AgsI	TTSAA	8 cut(s)	186, 233, 326, 419, 764, 1079, 1264, 1550
AjiI	CACGTC	1 cut(s)	335
AjnI	CCWGG	3 cut(s)	374, 735, 1680
AjuI	GAANNNNNNNTTGG	2 cut(s)	941, 973
AloI	GAACNNNNNNTCC	2 cut(s)	569, 601
Alw21I	GWGCWC	3 cut(s)	998, 1506, 1721
Alw26I	GTCTC	3 cut(s)	357, 517, 1244
AoxI	GGCC	6 cut(s)	154, 226, 732, 766, 1318, 1432
ApeKI	GCWGC	3 cut(s)	329, 899, 1697
ApoI	RAATTY	5 cut(s)	311, 379, 1599, 1655, 1815
Asp700I	GAANNNNTTC	1 cut(s)	1049
AspLEI	GCGC	3 cut(s)	506, 1121, 1301
AspS9I	GGNCC	1 cut(s)	733
AsuC2I	CCSGG	2 cut(s)	275, 749
AsuHPI	GGTGA	2 cut(s)	364, 1774
BanII	GRGCYC	3 cut(s)	263, 552, 998
BbsI	GAAGAC	1 cut(s)	1122
Bbv12I	GWGCWC	3 cut(s)	998, 1506, 1721
BbvI	GCAGC	3 cut(s)	341, 911, 1684
BccI	CCATC	3 cut(s)	153, 377, 631
BceAI	ACGGC	1 cut(s)	612
BciT130I	CCWGG	3 cut(s)	376, 737, 1682
BciVI	GTATCC	1 cut(s)	292
BclI	TGATCA	1 cut(s)	21
BcnI	CCSGG	2 cut(s)	275, 749
BcoDI	GTCTC	3 cut(s)	357, 517, 1244
BfaI	CTAG	3 cut(s)	1436, 1565, 1596
BfrI	CTTAAG	1 cut(s)	437
BfuAI	ACCTGC	1 cut(s)	748
BfuI	GTATCC	1 cut(s)	292
BglI	GCCNNNNNGGC	1 cut(s)	225
BisI	GCNGC	3 cut(s)	330, 900, 1698
BlpI	GCTNAGC	1 cut(s)	895
BlsI	GCNGC	3 cut(s)	331, 901, 1699
BmcAI	AGTACT	1 cut(s)	1594
Bme1390I	CCNGG	5 cut(s)	275, 376, 737, 749, 1682
BmgBI	CACGTC	1 cut(s)	335
BmgT120I	GGNCC	1 cut(s)	733
BmiI	GGNNCC	4 cut(s)	32, 262, 1383, 1686
BmrFI	CCNGG	5 cut(s)	275, 376, 737, 749, 1682
BmrI	ACTGGG	1 cut(s)	539
BmsI	GCATC	7 cut(s)	13, 421, 695, 1260, 1353, 1378, 1543
BmuI	ACTGGG	1 cut(s)	539
BpiI	GAAGAC	1 cut(s)	1122
BplI	GAGNNNNNCTC	2 cut(s)	1000, 1032
BpmI	CTGGAG	1 cut(s)	1703
Bpu1102I	GCTNAGC	1 cut(s)	895
BpuEI	CTTGAG	2 cut(s)	236, 1226
BpuMI	CCSGG	2 cut(s)	275, 749
Bsa29I	ATCGAT	1 cut(s)	1521
BsaBI	GATNNNNATC	1 cut(s)	1251
BsaJI	CCNNGG	7 cut(s)	99, 151, 735, 736, 1141, 1156, 1680
BsaWI	WCCGGW	1 cut(s)	1736
BsaXI	ACNNNNNCTCC	4 cut(s)	126, 156, 569, 599
Bsc4I	CCNNNNNNNGG	3 cut(s)	375, 1327, 1743
Bse118I	RCCGGY	2 cut(s)	27, 1430
Bse1I	ACTGG	2 cut(s)	534, 1748
Bse3DI	GCAATG	4 cut(s)	216, 708, 759, 889
Bse8I	GATNNNNATC	1 cut(s)	1251
BseBI	CCWGG	3 cut(s)	376, 737, 1682
BseCI	ATCGAT	1 cut(s)	1521
BseDI	CCNNGG	7 cut(s)	99, 151, 735, 736, 1141, 1156, 1680
BseGI	GGATG	2 cut(s)	145, 710
BseJI	GATNNNNATC	1 cut(s)	1251
BseLI	CCNNNNNNNGG	3 cut(s)	375, 1327, 1743
BseMI	GCAATG	4 cut(s)	216, 708, 759, 889
BseMII	CTCAG	5 cut(s)	78, 282, 909, 1680, 1706
BseNI	ACTGG	2 cut(s)	534, 1748
BseRI	GAGGAG	1 cut(s)	1780
BseXI	GCAGC	3 cut(s)	341, 911, 1684
Bsh1236I	CGCG	2 cut(s)	506, 627
Bsh1285I	CGRYCG	1 cut(s)	279
BshFI	GGCC	6 cut(s)	156, 228, 734, 768, 1320, 1434
BshVI	ATCGAT	1 cut(s)	1521
BsiEI	CGRYCG	1 cut(s)	279
BsiHKAI	GWGCWC	3 cut(s)	998, 1506, 1721
BsiSI	CCGG	5 cut(s)	28, 275, 748, 1431, 1737
BslFI	GGGAC	2 cut(s)	199, 837
BslI	CCNNNNNNNGG	3 cut(s)	375, 1327, 1743
BsmAI	GTCTC	3 cut(s)	357, 517, 1244
BsmBI	CGTCTC	1 cut(s)	517
BsmFI	GGGAC	2 cut(s)	199, 837
BsmI	GAATGC	2 cut(s)	1210, 1699
BsnI	GGCC	6 cut(s)	156, 228, 734, 768, 1320, 1434
Bsp1286I	GDGCHC	5 cut(s)	263, 552, 998, 1506, 1721
Bsp143I	GATC	4 cut(s)	21, 60, 1522, 1571
Bsp1720I	GCTNAGC	1 cut(s)	895
Bsp19I	CCATGG	2 cut(s)	151, 1156
Bsp68I	TCGCGA	1 cut(s)	627
BspACI	CCGC	2 cut(s)	51, 217
BspANI	GGCC	6 cut(s)	156, 228, 734, 768, 1320, 1434
BspCNI	CTCAG	5 cut(s)	77, 281, 908, 1681, 1707
BspDI	ATCGAT	1 cut(s)	1521
BspFNI	CGCG	2 cut(s)	506, 627
BspLI	GGNNCC	4 cut(s)	32, 262, 1383, 1686
BspMI	ACCTGC	1 cut(s)	748
BspTI	CTTAAG	1 cut(s)	437
BsrDI	GCAATG	4 cut(s)	216, 708, 759, 889
BsrFI	RCCGGY	2 cut(s)	27, 1430
BsrI	ACTGG	2 cut(s)	534, 1748
BssAI	RCCGGY	2 cut(s)	27, 1430
BssECI	CCNNGG	7 cut(s)	99, 151, 735, 736, 1141, 1156, 1680
BssMI	GATC	4 cut(s)	21, 60, 1522, 1571
BssT1I	CCWWGG	4 cut(s)	99, 151, 1141, 1156
Bst2UI	CCWGG	3 cut(s)	376, 737, 1682
Bst4CI	ACNGT	5 cut(s)	19, 322, 790, 1096, 1723
Bst6I	CTCTTC	2 cut(s)	1055, 1752
BstAFI	CTTAAG	1 cut(s)	437
BstC8I	GCNNGC	9 cut(s)	199, 487, 795, 1065, 1420, 1432, 1513, 1544, 1624
BstDEI	CTNAG	6 cut(s)	64, 268, 694, 895, 1689, 1715
BstDSI	CCRYGG	2 cut(s)	151, 1156
BstF5I	GGATG	2 cut(s)	145, 710
BstFNI	CGCG	2 cut(s)	506, 627
BstHHI	GCGC	3 cut(s)	506, 1121, 1301
BstKTI	GATC	4 cut(s)	24, 63, 1525, 1574
BstMAI	GTCTC	3 cut(s)	357, 517, 1244
BstMBI	GATC	4 cut(s)	21, 60, 1522, 1571
BstMCI	CGRYCG	1 cut(s)	279
BstMWI	GCNNNNNNNGC	3 cut(s)	225, 409, 1317
BstNI	CCWGG	3 cut(s)	376, 737, 1682
BstNSI	RCATGY	2 cut(s)	650, 916
BstSCI	CCNGG	5 cut(s)	273, 374, 735, 747, 1680
BstUI	CGCG	2 cut(s)	506, 627
BstV1I	GCAGC	3 cut(s)	341, 911, 1684
BstV2I	GAAGAC	1 cut(s)	1122
BstXI	CCANNNNNNTGG	1 cut(s)	152
Bsu15I	ATCGAT	1 cut(s)	1521
BsuI	GTATCC	1 cut(s)	292
BsuRI	GGCC	6 cut(s)	156, 228, 734, 768, 1320, 1434
BsuTUI	ATCGAT	1 cut(s)	1521
BtgI	CCRYGG	2 cut(s)	151, 1156
BtrI	CACGTC	1 cut(s)	335
BtsCI	GGATG	2 cut(s)	145, 710
BtsI	GCAGTG	1 cut(s)	238
BtsIMutI	CAGTG	3 cut(s)	238, 1092, 1301
BtuMI	TCGCGA	1 cut(s)	627
BveI	ACCTGC	1 cut(s)	748
Cac8I	GCNNGC	9 cut(s)	199, 487, 795, 1065, 1420, 1432, 1513, 1544, 1624
CfoI	GCGC	3 cut(s)	506, 1121, 1301
Cfr10I	RCCGGY	2 cut(s)	27, 1430
Cfr13I	GGNCC	1 cut(s)	733
ClaI	ATCGAT	1 cut(s)	1521
CseI	GACGC	2 cut(s)	232, 1454
Csp6I	GTAC	3 cut(s)	304, 464, 1593
CviQI	GTAC	3 cut(s)	304, 464, 1593
DdeI	CTNAG	6 cut(s)	64, 268, 694, 895, 1689, 1715
DpnI	GATC	4 cut(s)	23, 62, 1524, 1573
DpnII	GATC	4 cut(s)	21, 60, 1522, 1571
DraI	TTTAAA	1 cut(s)	79
Eam1104I	CTCTTC	2 cut(s)	1055, 1752
EarI	CTCTTC	2 cut(s)	1055, 1752
Ecl136II	GAGCTC	1 cut(s)	996
Eco130I	CCWWGG	4 cut(s)	99, 151, 1141, 1156
Eco147I	AGGCCT	1 cut(s)	768
Eco24I	GRGCYC	3 cut(s)	263, 552, 998
Eco53kI	GAGCTC	1 cut(s)	996
Eco57I	CTGAAG	2 cut(s)	1036, 1089
EcoICRI	GAGCTC	1 cut(s)	996
EcoRI	GAATTC	1 cut(s)	379
EcoRII	CCWGG	3 cut(s)	374, 735, 1680
EcoT14I	CCWWGG	4 cut(s)	99, 151, 1141, 1156
EcoT22I	ATGCAT	4 cut(s)	6, 450, 710, 856
EcoT38I	GRGCYC	3 cut(s)	263, 552, 998
ErhI	CCWWGG	4 cut(s)	99, 151, 1141, 1156
Esp3I	CGTCTC	1 cut(s)	517
FaqI	GGGAC	2 cut(s)	199, 837
FauI	CCCGC	2 cut(s)	58, 224
FauNDI	CATATG	1 cut(s)	660
FbaI	TGATCA	1 cut(s)	21
FblI	GTMKAC	1 cut(s)	593
Fnu4HI	GCNGC	3 cut(s)	330, 900, 1698
FokI	GGATG	2 cut(s)	132, 717
FriOI	GRGCYC	3 cut(s)	263, 552, 998
Fsp4HI	GCNGC	3 cut(s)	330, 900, 1698
FspBI	CTAG	3 cut(s)	1436, 1565, 1596
GlaI	GCGC	3 cut(s)	505, 1120, 1300
GluI	GCNGC	3 cut(s)	330, 900, 1698
GsuI	CTGGAG	1 cut(s)	1703
HaeIII	GGCC	6 cut(s)	156, 228, 734, 768, 1320, 1434
HapII	CCGG	5 cut(s)	28, 275, 748, 1431, 1737
HgaI	GACGC	2 cut(s)	232, 1454
HhaI	GCGC	3 cut(s)	506, 1121, 1301
Hin6I	GCGC	3 cut(s)	504, 1119, 1299
HinP1I	GCGC	3 cut(s)	504, 1119, 1299
HincII	GTYRAC	1 cut(s)	594
HindII	GTYRAC	1 cut(s)	594
HindIII	AAGCTT	2 cut(s)	195, 1544
HpaII	CCGG	5 cut(s)	28, 275, 748, 1431, 1737
HphI	GGTGA	2 cut(s)	364, 1774
Hpy166II	GTNNAC	1 cut(s)	594
Hpy188I	TCNGA	4 cut(s)	265, 271, 1336, 1470
Hpy188III	TCNNGA	4 cut(s)	416, 513, 626, 702
Hpy8I	GTNNAC	1 cut(s)	594
Hpy99I	CGWCG	2 cut(s)	248, 598
HpyCH4III	ACNGT	5 cut(s)	19, 322, 790, 1096, 1723
HpyCH4IV	ACGT	1 cut(s)	334
HpyF10VI	GCNNNNNNNGC	3 cut(s)	225, 409, 1317
HpyF3I	CTNAG	6 cut(s)	64, 268, 694, 895, 1689, 1715
HpySE526I	ACGT	1 cut(s)	334
HspAI	GCGC	3 cut(s)	504, 1119, 1299
KroI	GCCGGC	1 cut(s)	1430
KroNI	GCCGGC	1 cut(s)	1432
Ksp22I	TGATCA	1 cut(s)	21
Kzo9I	GATC	4 cut(s)	21, 60, 1522, 1571
LmnI	GCTCC	8 cut(s)	206, 266, 993, 1008, 1296, 1381, 1501, 1684
Lsp1109I	GCAGC	3 cut(s)	341, 911, 1684
LweI	GCATC	7 cut(s)	13, 421, 695, 1260, 1353, 1378, 1543
MaeI	CTAG	3 cut(s)	1436, 1565, 1596
MaeII	ACGT	1 cut(s)	334
MaeIII	GTNAC	6 cut(s)	13, 784, 1090, 1393, 1615, 1675
MalI	GATC	4 cut(s)	23, 62, 1524, 1573
MboI	GATC	4 cut(s)	21, 60, 1522, 1571
MboII	GAAGA	8 cut(s)	170, 1042, 1122, 1454, 1517, 1562, 1650, 1769
MhlI	GDGCHC	5 cut(s)	263, 552, 998, 1506, 1721
MlyI	GAGTC	3 cut(s)	260, 587, 1161
MmeI	TCCRAC	1 cut(s)	288
MnlI	CCTC	6 cut(s)	501, 612, 1367, 1412, 1753, 1758
Mph1103I	ATGCAT	4 cut(s)	6, 450, 710, 856
MroNI	GCCGGC	1 cut(s)	1430
MroXI	GAANNNNTTC	1 cut(s)	1049
MslI	CAYNNNNRTG	8 cut(s)	9, 150, 299, 453, 723, 911, 1161, 1217
MspCI	CTTAAG	1 cut(s)	437
MspI	CCGG	5 cut(s)	28, 275, 748, 1431, 1737
MspR9I	CCNGG	5 cut(s)	275, 376, 737, 749, 1682
Mva1269I	GAATGC	2 cut(s)	1210, 1699
MvaI	CCWGG	3 cut(s)	376, 737, 1682
MvnI	CGCG	2 cut(s)	506, 627
MwoI	GCNNNNNNNGC	3 cut(s)	225, 409, 1317
NaeI	GCCGGC	1 cut(s)	1432
NciI	CCSGG	2 cut(s)	275, 749
NcoI	CCATGG	2 cut(s)	151, 1156
NdeI	CATATG	1 cut(s)	660
NdeII	GATC	4 cut(s)	21, 60, 1522, 1571
NgoMIV	GCCGGC	1 cut(s)	1430
NlaIV	GGNNCC	4 cut(s)	32, 262, 1383, 1686
NmeAIII	GCCGAG	2 cut(s)	841, 1299
NmuCI	GTSAC	3 cut(s)	13, 784, 1090
NruI	TCGCGA	1 cut(s)	627
NsiI	ATGCAT	4 cut(s)	6, 450, 710, 856
NspI	RCATGY	2 cut(s)	650, 916
PasI	CCCWGGG	1 cut(s)	736
PceI	AGGCCT	1 cut(s)	768
PciI	ACATGT	2 cut(s)	646, 912
PctI	GAATGC	2 cut(s)	1210, 1699
PdiI	GCCGGC	1 cut(s)	1432
PdmI	GAANNNNTTC	1 cut(s)	1049
PfeI	GAWTC	8 cut(s)	541, 847, 878, 1247, 1283, 1331, 1630, 1806
PflFI	GACNNNGTC	1 cut(s)	275
PflMI	CCANNNNNTGG	1 cut(s)	375
PkrI	GCNGC	3 cut(s)	331, 901, 1699
PleI	GAGTC	3 cut(s)	260, 586, 1160
PpsI	GAGTC	3 cut(s)	260, 586, 1160
PscI	ACATGT	2 cut(s)	646, 912
Psp124BI	GAGCTC	1 cut(s)	998
Psp6I	CCWGG	3 cut(s)	374, 735, 1680
PspGI	CCWGG	3 cut(s)	374, 735, 1680
PspN4I	GGNNCC	4 cut(s)	32, 262, 1383, 1686
PspPI	GGNCC	1 cut(s)	733
PsyI	GACNNNGTC	1 cut(s)	275
RruI	TCGCGA	1 cut(s)	627
RsaI	GTAC	3 cut(s)	305, 465, 1594
RsaNI	GTAC	3 cut(s)	304, 464, 1593
RseI	CAYNNNNRTG	8 cut(s)	9, 150, 299, 453, 723, 911, 1161, 1217
SacI	GAGCTC	1 cut(s)	998
SalI	GTCGAC	1 cut(s)	592
SatI	GCNGC	3 cut(s)	330, 900, 1698
Sau3AI	GATC	4 cut(s)	21, 60, 1522, 1571
Sau96I	GGNCC	1 cut(s)	733
ScaI	AGTACT	1 cut(s)	1594
SchI	GAGTC	3 cut(s)	260, 587, 1161
ScrFI	CCNGG	5 cut(s)	275, 376, 737, 749, 1682
SduI	GDGCHC	5 cut(s)	263, 552, 998, 1506, 1721
SfaNI	GCATC	7 cut(s)	13, 421, 695, 1260, 1353, 1378, 1543
SmiMI	CAYNNNNRTG	8 cut(s)	9, 150, 299, 453, 723, 911, 1161, 1217
SmlI	CTYRAG	3 cut(s)	251, 437, 1241
SmoI	CTYRAG	3 cut(s)	251, 437, 1241
SseBI	AGGCCT	1 cut(s)	768
SsiI	CCGC	2 cut(s)	51, 217
SspMI	CTAG	3 cut(s)	1436, 1565, 1596
SstI	GAGCTC	1 cut(s)	998
StuI	AGGCCT	1 cut(s)	768
StyD4I	CCNGG	5 cut(s)	273, 374, 735, 747, 1680
StyI	CCWWGG	4 cut(s)	99, 151, 1141, 1156
TaaI	ACNGT	5 cut(s)	19, 322, 790, 1096, 1723
TaiI	ACGT	1 cut(s)	337
TaqI	TCGA	4 cut(s)	593, 635, 960, 1521
TatI	WGTACW	1 cut(s)	1592
TfiI	GAWTC	8 cut(s)	541, 847, 878, 1247, 1283, 1331, 1630, 1806
TscAI	CASTG	3 cut(s)	245, 1099, 1308
TseFI	GTSAC	3 cut(s)	13, 784, 1090
TseI	GCWGC	3 cut(s)	329, 899, 1697
Tsp45I	GTSAC	3 cut(s)	13, 784, 1090
TspGWI	ACGGA	1 cut(s)	1818
TspRI	CASTG	3 cut(s)	245, 1099, 1308
Tth111I	GACNNNGTC	1 cut(s)	275
Van91I	CCANNNNNTGG	1 cut(s)	375
Vha464I	CTTAAG	1 cut(s)	437
XapI	RAATTY	5 cut(s)	311, 379, 1599, 1655, 1815
XceI	RCATGY	2 cut(s)	650, 916
XmiI	GTMKAC	1 cut(s)	593
XmnI	GAANNNNTTC	1 cut(s)	1049
XspI	CTAG	3 cut(s)	1436, 1565, 1596
ZrmI	AGTACT	1 cut(s)	1594
Zsp2I	ATGCAT	4 cut(s)	6, 450, 710, 856

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.