pycom04g01050

Belongs to the glycosyltransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011248
Physical Location & Seq
Reverse (-)
950644 .. 955905
5262 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3201 bp
ATGGCCGGAAACGAGTGGATTAATGGGTACTTGGAGGCGATACTGGACAGCGGCGCCTCCGCCATTGAGGAGCAGAAGCCGGTGCCGGAGAATCTGAGAGATAGAGGACATTTCAATCCGACCAAGTACTTCGTGGAGGAGGTGGTGACGGGGGTCGATGAGTCGGACCTTTACCGGACGTGGATCAAGGTGGTTGCGACCCGCAACACGCGGGAACGGGGCTCCAGGCTCGAGAATATGTGCTGGCGCATTTGGCACCTTACCCGTAAGAAGAAACAGTTGGAGGTGGAGGAACATCAGCGGTTGGCAAACCGCAGATGGGAGCGGGAACAAGGACGTAGGGATGCAACTGAAGACATGTCGGAGGACTTGTCTGAAGGAGAGAAAGGGGATGGTTTGGGGGAGATGCAACTAGGTGATACACCAAGGAAAAGGTTCCAGCGTAATATTTCGAACTTGGAAGTATGGTCAGACGATAAGAAGGAAAAGAAGCTTTACATTGTTCTTATCAGTTTGCATGGTTTGGTCCGGGGAGAAAACATGGAGCTCGGTCGAGACTCTGATACAGGTGGACAGGTCAAATATGTGGTAGAGCTTTCCCGAGCTCTTGCGAGGATGCCTGGGGTTTATAGGGTAGATCTCTTTACGCGCCAAGTGTCATGTCCTGATGTTGATTGGAGCTATGGTGAGCCAACAGAGATGCTAACTGCAGGTCCTGAAGATGGTGATGGTGGTCTAGGAGAAAGCAGTGGAGCATATATTATTAGAATCCCTTTCGGACCCCGTGATCAATATCTCAGCAAAGAAGTACTCTGGCCATATATTCAAGAATTTGTAGATGGTGCTCTAGCTCACATTCTTAACATGTCCAAAGTTTTGGGAGAACAGATTGGTAAAGGACAGCCTGTCTGGCCGTATGTAATTCATGGCCACTACGCAGATGCAGGGGATAGTGCTGCTCTTCTTTCTGGTGCTTTGAATGTCCCAATGGTTTTAACTGGGCACTCACTTGGAAGAAACAAGCTTGAACAGCTTCTTAAGCAGGGACGCCAATCAAAGGAGGACATCAATTCTACATATAAAATTATGAGGAGGATTGAAGCAGAAGAACTTTCCCTTGATGCTGCAGAACTAGTTATTACAAGTACAAAACAAGAGAGCGAAGAGCAATGGGGGCTTTACGATGGGTTTGATGTCAAGCTTGAGAAAGTTTTGCGTGCTCGTGCTAGGCGAGGAGTAAATTGCCATGGCCGTTACATGCCAAGGATGGTGGTTATACCTCCTGGCATGGACTTCAGTAATGTTGTGGTTCAAGAAGATACGCCTGAGGTTGATGGCGAACTTAACCAACTTATTGGTGGTACTGATGGGTCTTCCCCGAAAGCAATTCCAACAATATGGTCAGAAGTGATGCGGTTCCTTACAAATCCTCACAAGCCAATGATCTTGGCTTTGTCAAGACCAGATCCGAAGAAGAACATAACCACTCTTTTAAAAGCCTTTGGGGAATGCGGTCCTCTAAGAGAGCTCGCTAATCTTACACTGATAATGGGGAATAGGGACTGCATAGATGAGATGTCTACCGGAAATGCTAGTGTTCTTACGACAGTTTTGAAACTAATTGATAAGTATGACCTTTATGGGCAAGTGGCATATCCAAAGCATCACAGGCAATCAGATGTTCCAGATATATACCGTCTCACGGCAAAAACAAAGGGTGTTTTCATAAATCCAGCATTAGTGGAGCCTTTTGGGCTAACCTTGATTGAGGCAGCAGCACATGGGCTTCCAATGGTGGCTACAAAAAACGGTGGACCGGTTGATATACATCGGGCGCTGAATAATGGTTTGCTTGTGGACCCTCATGATCAGCAGTCGATTGCTGGTGCACTTCTTAAGTTGTTATCAGAGAAGAATTTATGGAGTGAATGTAGAAAGAATGGCTGGAAGAACATACACCTCTACTCATGGCCTGAACATTGCCGGACTTACCTGACTAGGGTTGCAGCCTGCCGCATGAGACATCCACAATGGCAAACTGACACCCCAGAGGATGATATGGCTGCTGACGAGTCTCTCAATGATTCTCTTAAGGATGTGCAAGATATGTCTCTTAGGCTCTCAGTTGATGGGGATAAAGCTTCATTGAATGGATCTTTTGATGTGACTGCAGCCACTGGTGACCACGACGTGCAAGATCAAGTGAAACGTGTCCTAAGCAAGATCAAGAAACCAGATTCCGGTCCAAAAGATCAAGATGTCGGAAATAAGCCGTTGGATAATGTGTCAAGTAAATATCCCATGTTAAGGAGGAGACGGAAATTGATTGTTATAGCGCTTGATTGCTACGAAAGCTCTGGAGATCCTAAAATGAAAATGATTCAGGTAGTTCAAGAGATATTTAAGGCTGTCAGACTAGACTCTCAAAGTGCTAGGGTCACAGGGTTTGCTTTATTAACGGCTATGCCAATGTCAGAAACTGTAGAGTTTTTGGCATCAGGGAAGATACAGGCAAACGAATTTGACGCATTGGTTTGTAGCAGTGGAAGTGAAGTTTACTACCCTGGTACTTATACAGAAGGCGGCGGAAGACTTTCCCCAGATCCAGATTATGCATCACATATTGACTACCGTTGGGGTTGTGAGGGTCTAAAGAAAACAATTTTGAAGCTGTTGAATGCCCCTGAAGGCAAAGGAAATTCTGCTTCTTCCAGCCACATTCAAGAAGACTTGAAATCAAGCAACGCACATTGCATCTCATTCTTCATAAAGGATCCTAGTAAGGCAAGGAAAGTTGATGATTTGAGGCAGAAACTCAGGATGCGGGGACTGCGCTGCCATCCAATGTACTCCAGGAGCTCAACGAGAATGCAAATTGTTCCTCTCCTTGCTTCTCGAGCGCAGGCACTCAGGTACCTTTTTGTTCGTTGGAGATTGAATGTCGCCAACATGTATGTGTTCCTTGGAGAAAGTGGAGACACCGATTATGAAGAAATGATATCTGGAACTCATAAGACAATAATCATGAAAGGGGTGGTGGCAAAGGGTTCGGAAGAGCTGCTAAGAACATCAGGAAGCTATCTCAAAGACGACATTGTTCCTCCCGAGAGCCCTTTGGTCGCCTACGTAAGCGAGGAAACAAAAGCTGATGAGATTGCTAATGCTCTGAAGCAAGTCTCCAAGTCTGCTGCTGGAATGTGA

Protein Analysis

1067

Amino Acids

119.28

Weight (kDa)

6.24

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_trans_4_4 PF13579 190 - 388 8.2e-06 Glycosyl transferase 4-like domain
GT-B_Sucrose_synth PF00862 251 - 432 2.9e-08 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 476 - 648 2.5e-22 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 480 - 636 1.5e-13 Glycosyl transferases group 1
S6PP PF05116 816 - 1008 2.8e-15 Sucrose-6F-phosphate phosphohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011248)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04920
fragaria_vesca FvH4_4g13520 FvH4_4g13520 FvH4_4g13520
malus_domestica MD04G1013500.v1.1
prunus_persica Prupe.1G159700_v2.0.a1 Prupe.1G159700_v2.0.a1 Prupe.1G159700_v2.0.a1
pyrus_communis pycom04g01050
rosa_chinensis RchiOBHm_Chr4g0414151
rosa_laevigata RLG00000008166
rosa_multiflora Rmu_sc0001063.1_g000015
rosa_roxburghii Rroxscaffold_5G00358160
rosa_rugosa Rorug04G0126600
rosa_samantha Rh4AG184700 Rh4BG184100 Rh4CG196400 Rh4DG180000
rosa_wichuraiana Rw0G005040 Rw4G015730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 701
Acc65I GGTACC 1 cut(s) 2913
AccB1I GGYRCC 4 cut(s) 53, 82, 255, 2913
AccBSI CCGCTC 1 cut(s) 325
AccI GTMKAC 1 cut(s) 1580
AccII CGCG 2 cut(s) 211, 649
AclWI GGATC 7 cut(s) 191, 1460, 2161, 2357, 2597, 2768, 2781
AcoI YGGCCR 5 cut(s) 3, 815, 911, 928, 1249
AcsI RAATTY 4 cut(s) 830, 1915, 2519, 2698
AcuI CTGAAG 6 cut(s) 372, 396, 738, 1279, 2706, 3188
AcyI GRCGYC 2 cut(s) 54, 1048
AdeI CACNNNGTG 1 cut(s) 2191
AfaI GTAC 8 cut(s) 29, 128, 810, 1147, 1363, 2569, 2849, 2915
AfeI AGCGCT 1 cut(s) 2337
AfiI CCNNNNNNNGG 7 cut(s) 319, 722, 1057, 1702, 1999, 2240, 2307
AflII CTTAAG 3 cut(s) 1037, 1895, 2090
AflIII ACRYGT 4 cut(s) 357, 864, 2209, 2949
AgeI ACCGGT 1 cut(s) 1816
AhdI GACNNNNNGTC 1 cut(s) 905
AhlI ACTAGT 1 cut(s) 1132
AjiI CACGTC 2 cut(s) 180, 2191
AjnI CCWGG 5 cut(s) 224, 619, 1282, 2563, 2852
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
Alw21I GWGCWC 7 cut(s) 549, 607, 847, 1222, 1530, 1891, 2861
Alw26I GTCTC 8 cut(s) 549, 1703, 2014, 2079, 2115, 2308, 2970, 3181
Alw44I GTGCAC 1 cut(s) 1887
AlwI GGATC 7 cut(s) 191, 1460, 2161, 2357, 2597, 2768, 2781
AlwNI CAGNNNCTG 3 cut(s) 716, 2177, 2480
Ama87I CYCGRG 4 cut(s) 230, 600, 2895, 3104
Aor51HI AGCGCT 1 cut(s) 2337
AoxI GGCC 6 cut(s) 3, 815, 911, 928, 1249, 1970
ApaLI GTGCAC 1 cut(s) 1887
ApoI RAATTY 4 cut(s) 830, 1915, 2519, 2698
AseI ATTAAT 1 cut(s) 21
AsiGI ACCGGT 1 cut(s) 1816
Asp700I GAANNNNTTC 3 cut(s) 434, 1032, 2593
Asp718I GGTACC 1 cut(s) 2913
AspLEI GCGC 7 cut(s) 56, 249, 651, 1837, 2338, 2835, 2902
AspS9I GGNCC 8 cut(s) 166, 526, 713, 779, 1514, 1814, 1858, 2243
AsuC2I CCSGG 1 cut(s) 530
AsuHPI GGTGA 5 cut(s) 157, 428, 698, 737, 2192
AsuII TTCGAA 1 cut(s) 452
AvaI CYCGRG 4 cut(s) 230, 600, 2895, 3104
AvaII GGWCC 8 cut(s) 166, 526, 713, 779, 1514, 1814, 1858, 2243
AxyI CCTNAGG 1 cut(s) 1326
BaeGI GKGCMC 2 cut(s) 1005, 1891
BaeI ACNNNNGTAYC 2 cut(s) 2897, 2930
BalI TGGCCA 2 cut(s) 817, 930
BamHI GGATCC 1 cut(s) 2773
BanI GGYRCC 4 cut(s) 53, 82, 255, 2913
BanII GRGCYC 6 cut(s) 224, 549, 607, 1530, 2861, 3113
BauI CACGAG 1 cut(s) 1221
BbsI GAAGAC 4 cut(s) 360, 1365, 2596, 2733
Bbv12I GWGCWC 7 cut(s) 549, 607, 847, 1222, 1530, 1891, 2861
BceAI ACGGC 5 cut(s) 898, 1236, 1719, 2257, 2475
BcgI CGANNNNNNTGC 2 cut(s) 3030, 3064
BciT130I CCWGG 5 cut(s) 226, 621, 1284, 2565, 2854
BclI TGATCA 2 cut(s) 787, 1867
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 8 cut(s) 549, 1703, 2014, 2079, 2115, 2308, 2970, 3181
BcuI ACTAGT 1 cut(s) 1132
BfmI CTRYAG 4 cut(s) 708, 1125, 2169, 2481
BfoI RGCGCY 3 cut(s) 57, 1838, 2339
BfrI CTTAAG 3 cut(s) 1037, 1895, 2090
BfuAI ACCTGC 1 cut(s) 701
BglI GCCNNNNNGGC 2 cut(s) 910, 1753
BglII AGATCT 1 cut(s) 637
BmcAI AGTACT 2 cut(s) 128, 810
Bme1390I CCNGG 6 cut(s) 226, 530, 621, 1284, 2565, 2854
Bme18I GGWCC 8 cut(s) 166, 526, 713, 779, 1514, 1814, 1858, 2243
BmeRI GACNNNNNGTC 1 cut(s) 905
BmeT110I CYCGRG 4 cut(s) 230, 600, 2895, 3104
BmgBI CACGTC 2 cut(s) 180, 2191
BmgT120I GGNCC 8 cut(s) 166, 526, 713, 779, 1514, 1814, 1858, 2243
BmrFI CCNGG 6 cut(s) 226, 530, 621, 1284, 2565, 2854
BmrI ACTGGG 1 cut(s) 1008
BmuI ACTGGG 1 cut(s) 1008
BoxI GACNNNNGTC 1 cut(s) 152
BpiI GAAGAC 4 cut(s) 360, 1365, 2596, 2733
BpmI CTGGAG 3 cut(s) 208, 2379, 2836
Bpu10I CCTNAGC 1 cut(s) 2216
Bpu14I TTCGAA 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 1223
BpuMI CCSGG 1 cut(s) 530
BsaAI YACGTR 1 cut(s) 3127
BsaBI GATNNNNATC 2 cut(s) 792, 1827
BsaHI GRCGYC 2 cut(s) 54, 1048
BsaJI CCNNGG 7 cut(s) 425, 529, 620, 1246, 1262, 2563, 2962
BsaWI WCCGGW 4 cut(s) 174, 1583, 1816, 2240
BsaXI ACNNNNNCTCC 6 cut(s) 356, 386, 670, 700, 1915, 1945
Bsc4I CCNNNNNNNGG 7 cut(s) 319, 722, 1057, 1702, 1999, 2240, 2307
Bse118I RCCGGY 2 cut(s) 79, 1816
Bse1I ACTGG 3 cut(s) 48, 1003, 2182
Bse21I CCTNAGG 1 cut(s) 1326
Bse3DI GCAATG 3 cut(s) 1175, 1978, 2749
Bse8I GATNNNNATC 2 cut(s) 792, 1827
BseBI CCWGG 5 cut(s) 226, 621, 1284, 2565, 2854
BseDI CCNNGG 7 cut(s) 425, 529, 620, 1246, 1262, 2563, 2962
BseGI GGATG 9 cut(s) 349, 397, 621, 1272, 2023, 2059, 2101, 2826, 2839
BseJI GATNNNNATC 2 cut(s) 792, 1827
BseLI CCNNNNNNNGG 7 cut(s) 319, 722, 1057, 1702, 1999, 2240, 2307
BseMI GCAATG 3 cut(s) 1175, 1978, 2749
BseMII CTCAG 6 cut(s) 86, 811, 1317, 2136, 2830, 2923
BseNI ACTGG 3 cut(s) 48, 1003, 2182
BseRI GAGGAG 5 cut(s) 83, 152, 1105, 1248, 2326
BseSI GKGCMC 2 cut(s) 1005, 1891
Bsh1236I CGCG 2 cut(s) 211, 649
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 6 cut(s) 5, 817, 913, 930, 1251, 1972
BshNI GGYRCC 4 cut(s) 53, 82, 255, 2913
BshTI ACCGGT 1 cut(s) 1816
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 7 cut(s) 549, 607, 847, 1222, 1530, 1891, 2861
BsiHKCI CYCGRG 4 cut(s) 230, 600, 2895, 3104
BsiSI CCGG 9 cut(s) 6, 80, 86, 175, 529, 1584, 1817, 1984, 2241
BslFI GGGAC 4 cut(s) 968, 1059, 1574, 2841
BslI CCNNNNNNNGG 7 cut(s) 319, 722, 1057, 1702, 1999, 2240, 2307
BsmAI GTCTC 8 cut(s) 549, 1703, 2014, 2079, 2115, 2308, 2970, 3181
BsmBI CGTCTC 2 cut(s) 1703, 2308
BsmFI GGGAC 4 cut(s) 968, 1059, 1574, 2841
BsmI GAATGC 3 cut(s) 1514, 2683, 2874
BsnI GGCC 6 cut(s) 5, 817, 913, 930, 1251, 1972
BsoBI CYCGRG 4 cut(s) 230, 600, 2895, 3104
Bsp119I TTCGAA 1 cut(s) 452
Bsp19I CCATGG 1 cut(s) 1246
BspANI GGCC 6 cut(s) 5, 817, 913, 930, 1251, 1972
BspCNI CTCAG 6 cut(s) 87, 810, 1318, 2135, 2829, 2922
BspFNI CGCG 2 cut(s) 211, 649
BspHI TCATGA 2 cut(s) 1864, 3024
BspMAI CTGCAG 3 cut(s) 712, 1129, 2173
BspMI ACCTGC 1 cut(s) 701
BspPI GGATC 7 cut(s) 191, 1460, 2161, 2357, 2597, 2768, 2781
BspQI GCTCTTC 3 cut(s) 966, 1158, 3048
BspT104I TTCGAA 1 cut(s) 452
BspT107I GGYRCC 4 cut(s) 53, 82, 255, 2913
BspTI CTTAAG 3 cut(s) 1037, 1895, 2090
BsrBI CCGCTC 1 cut(s) 325
BsrDI GCAATG 3 cut(s) 1175, 1978, 2749
BsrFI RCCGGY 2 cut(s) 79, 1816
BsrI ACTGG 3 cut(s) 48, 1003, 2182
BssAI RCCGGY 2 cut(s) 79, 1816
BssECI CCNNGG 7 cut(s) 425, 529, 620, 1246, 1262, 2563, 2962
BssNI GRCGYC 2 cut(s) 54, 1048
BssSI CACGAG 1 cut(s) 1221
BssT1I CCWWGG 4 cut(s) 425, 1246, 1262, 2962
Bst2BI CACGAG 1 cut(s) 1221
Bst2UI CCWGG 5 cut(s) 226, 621, 1284, 2565, 2854
Bst4CI ACNGT 6 cut(s) 279, 1609, 1697, 1811, 2482, 2633
Bst6I CTCTTC 3 cut(s) 966, 1158, 3048
BstACI GRCGYC 2 cut(s) 54, 1048
BstAFI CTTAAG 3 cut(s) 1037, 1895, 2090
BstBAI YACGTR 1 cut(s) 3127
BstBI TTCGAA 1 cut(s) 452
BstC8I GCNNGC 5 cut(s) 245, 1218, 1530, 2011, 2904
BstDSI CCRYGG 1 cut(s) 1246
BstEII GGTNACC 1 cut(s) 2180
BstF5I GGATG 9 cut(s) 349, 397, 621, 1272, 2023, 2059, 2101, 2826, 2839
BstFNI CGCG 2 cut(s) 211, 649
BstH2I RGCGCY 3 cut(s) 57, 1838, 2339
BstHHI GCGC 7 cut(s) 56, 249, 651, 1837, 2338, 2835, 2902
BstMAI GTCTC 8 cut(s) 549, 1703, 2014, 2079, 2115, 2308, 2970, 3181
BstMCI CGRYCG 1 cut(s) 553
BstNI CCWGG 5 cut(s) 226, 621, 1284, 2565, 2854
BstNSI RCATGY 4 cut(s) 361, 868, 1261, 2953
BstPAI GACNNNNGTC 1 cut(s) 152
BstPI GGTNACC 1 cut(s) 2180
BstSCI CCNGG 6 cut(s) 224, 528, 619, 1282, 2563, 2852
BstSFI CTRYAG 4 cut(s) 708, 1125, 2169, 2481
BstSLI GKGCMC 2 cut(s) 1005, 1891
BstSNI TACGTA 1 cut(s) 3127
BstUI CGCG 2 cut(s) 211, 649
BstV2I GAAGAC 4 cut(s) 360, 1365, 2596, 2733
BstX2I RGATCY 6 cut(s) 637, 1465, 2153, 2362, 2602, 2773
BstXI CCANNNNNNTGG 3 cut(s) 877, 1355, 1398
BstYI RGATCY 6 cut(s) 637, 1465, 2153, 2362, 2602, 2773
Bsu36I CCTNAGG 1 cut(s) 1326
BsuRI GGCC 6 cut(s) 5, 817, 913, 930, 1251, 1972
BtgI CCRYGG 1 cut(s) 1246
BtrI CACGTC 2 cut(s) 180, 2191
BtsCI GGATG 9 cut(s) 349, 397, 621, 1272, 2023, 2059, 2101, 2826, 2839
BtsI GCAGTG 2 cut(s) 754, 2548
BtsIMutI CAGTG 4 cut(s) 754, 1541, 2175, 2548
BveI ACCTGC 1 cut(s) 701
Cac8I GCNNGC 5 cut(s) 245, 1218, 1530, 2011, 2904
CaiI CAGNNNCTG 3 cut(s) 716, 2177, 2480
CciI TCATGA 2 cut(s) 1864, 3024
CfoI GCGC 7 cut(s) 56, 249, 651, 1837, 2338, 2835, 2902
Cfr10I RCCGGY 2 cut(s) 79, 1816
Cfr13I GGNCC 8 cut(s) 166, 526, 713, 779, 1514, 1814, 1858, 2243
CseI GACGC 2 cut(s) 1056, 2534
Csp6I GTAC 8 cut(s) 28, 127, 809, 1146, 1362, 2568, 2848, 2914
CspAI ACCGGT 1 cut(s) 1816
CspCI CAANNNNNGTGG 2 cut(s) 1251, 1286
CviQI GTAC 8 cut(s) 28, 127, 809, 1146, 1362, 2568, 2848, 2914
DinI GGCGCC 1 cut(s) 55
DraI TTTAAA 1 cut(s) 1494
DraIII CACNNNGTG 1 cut(s) 2191
DriI GACNNNNNGTC 1 cut(s) 905
EaeI YGGCCR 5 cut(s) 3, 815, 911, 928, 1249
Eam1104I CTCTTC 3 cut(s) 966, 1158, 3048
Eam1105I GACNNNNNGTC 1 cut(s) 905
EarI CTCTTC 3 cut(s) 966, 1158, 3048
EciI GGCGGA 2 cut(s) 49, 2601
Ecl136II GAGCTC 4 cut(s) 547, 605, 1528, 2859
Eco105I TACGTA 1 cut(s) 3127
Eco130I CCWWGG 4 cut(s) 425, 1246, 1262, 2962
Eco24I GRGCYC 6 cut(s) 224, 549, 607, 1530, 2861, 3113
Eco32I GATATC 1 cut(s) 3000
Eco47I GGWCC 8 cut(s) 166, 526, 713, 779, 1514, 1814, 1858, 2243
Eco47III AGCGCT 1 cut(s) 2337
Eco53kI GAGCTC 4 cut(s) 547, 605, 1528, 2859
Eco57I CTGAAG 6 cut(s) 372, 396, 738, 1279, 2706, 3188
Eco81I CCTNAGG 1 cut(s) 1326
Eco88I CYCGRG 4 cut(s) 230, 600, 2895, 3104
Eco91I GGTNACC 1 cut(s) 2180
EcoICRI GAGCTC 4 cut(s) 547, 605, 1528, 2859
EcoO109I RGGNCCY 1 cut(s) 713
EcoO65I GGTNACC 1 cut(s) 2180
EcoRII CCWGG 5 cut(s) 224, 619, 1282, 2563, 2852
EcoRV GATATC 1 cut(s) 3000
EcoT14I CCWWGG 4 cut(s) 425, 1246, 1262, 2962
EcoT22I ATGCAT 1 cut(s) 2617
EcoT38I GRGCYC 6 cut(s) 224, 549, 607, 1530, 2861, 3113
EgeI GGCGCC 1 cut(s) 55
EheI GGCGCC 1 cut(s) 55
ErhI CCWWGG 4 cut(s) 425, 1246, 1262, 2962
Esp3I CGTCTC 2 cut(s) 1703, 2308
FaqI GGGAC 4 cut(s) 968, 1059, 1574, 2841
FauI CCCGC 4 cut(s) 204, 209, 318, 2817
FbaI TGATCA 2 cut(s) 787, 1867
FblI GTMKAC 1 cut(s) 1580
FokI GGATG 9 cut(s) 356, 404, 628, 1279, 2010, 2066, 2108, 2826, 2833
FriOI GRGCYC 6 cut(s) 224, 549, 607, 1530, 2861, 3113
GlaI GCGC 7 cut(s) 55, 248, 650, 1836, 2337, 2834, 2901
GsuI CTGGAG 3 cut(s) 208, 2379, 2836
HaeII RGCGCY 3 cut(s) 57, 1838, 2339
HaeIII GGCC 6 cut(s) 5, 817, 913, 930, 1251, 1972
HapII CCGG 9 cut(s) 6, 80, 86, 175, 529, 1584, 1817, 1984, 2241
HgaI GACGC 2 cut(s) 1056, 2534
HhaI GCGC 7 cut(s) 56, 249, 651, 1837, 2338, 2835, 2902
Hin1I GRCGYC 2 cut(s) 54, 1048
Hin6I GCGC 7 cut(s) 54, 247, 649, 1835, 2336, 2833, 2900
HinP1I GCGC 7 cut(s) 54, 247, 649, 1835, 2336, 2833, 2900
HindIII AAGCTT 4 cut(s) 491, 1022, 1199, 2139
HinfI GANTC 9 cut(s) 91, 161, 557, 768, 2072, 2084, 2237, 2380, 2420
HpaII CCGG 9 cut(s) 6, 80, 86, 175, 529, 1584, 1817, 1984, 2241
HphI GGTGA 5 cut(s) 157, 428, 698, 737, 2192
Hpy166II GTNNAC 6 cut(s) 572, 1581, 1814, 1858, 1889, 2557
Hpy8I GTNNAC 6 cut(s) 572, 1581, 1814, 1858, 1889, 2557
Hpy99I CGWCG 1 cut(s) 2192
HpyAV CCTTC 4 cut(s) 371, 475, 2573, 2681
HpyCH4III ACNGT 6 cut(s) 279, 1609, 1697, 1811, 2482, 2633
HpyCH4IV ACGT 5 cut(s) 179, 337, 2190, 2209, 3126
HpySE526I ACGT 5 cut(s) 179, 337, 2190, 2209, 3126
Hsp92I GRCGYC 2 cut(s) 54, 1048
HspAI GCGC 7 cut(s) 54, 247, 649, 1835, 2336, 2833, 2900
KasI GGCGCC 1 cut(s) 53
KpnI GGTACC 1 cut(s) 2917
Ksp22I TGATCA 2 cut(s) 787, 1867
LguI GCTCTTC 3 cut(s) 966, 1158, 3048
LmnI GCTCC 8 cut(s) 70, 227, 322, 544, 678, 752, 1744, 2856
MaeII ACGT 5 cut(s) 179, 337, 2190, 2209, 3126
MaeIII GTNAC 5 cut(s) 145, 1253, 2164, 2180, 2437
MbiI CCGCTC 1 cut(s) 325
MflI RGATCY 6 cut(s) 637, 1465, 2153, 2362, 2602, 2773
MlsI TGGCCA 2 cut(s) 817, 930
MluNI TGGCCA 2 cut(s) 817, 930
Mly113I GGCGCC 1 cut(s) 54
MlyI GAGTC 4 cut(s) 170, 551, 2081, 2414
MmeI TCCRAC 8 cut(s) 143, 144, 261, 342, 1415, 2242, 2256, 2909
Mox20I TGGCCA 2 cut(s) 817, 930
Mph1103I ATGCAT 1 cut(s) 2617
MroXI GAANNNNTTC 3 cut(s) 434, 1032, 2593
MscI TGGCCA 2 cut(s) 817, 930
MslI CAYNNNNRTG 1 cut(s) 2954
Msp20I TGGCCA 2 cut(s) 817, 930
MspA1I CMGCKG 2 cut(s) 51, 301
MspCI CTTAAG 3 cut(s) 1037, 1895, 2090
MspI CCGG 9 cut(s) 6, 80, 86, 175, 529, 1584, 1817, 1984, 2241
MspR9I CCNGG 6 cut(s) 226, 530, 621, 1284, 2565, 2854
Mva1269I GAATGC 3 cut(s) 1514, 2683, 2874
MvaI CCWGG 5 cut(s) 226, 621, 1284, 2565, 2854
MvnI CGCG 2 cut(s) 211, 649
NarI GGCGCC 1 cut(s) 54
NciI CCSGG 1 cut(s) 530
NcoI CCATGG 1 cut(s) 1246
NmuCI GTSAC 4 cut(s) 145, 2164, 2180, 2437
NsiI ATGCAT 1 cut(s) 2617
NspI RCATGY 4 cut(s) 361, 868, 1261, 2953
NspV TTCGAA 1 cut(s) 452
PaeR7I CTCGAG 2 cut(s) 230, 2895
PagI TCATGA 2 cut(s) 1864, 3024
PciI ACATGT 3 cut(s) 357, 864, 2949
PciSI GCTCTTC 3 cut(s) 966, 1158, 3048
PcsI WCGNNNNNNNCGW 1 cut(s) 1228
PctI GAATGC 3 cut(s) 1514, 2683, 2874
PdmI GAANNNNTTC 3 cut(s) 434, 1032, 2593
PfeI GAWTC 5 cut(s) 91, 768, 2084, 2237, 2380
PfoI TCCNGGA 1 cut(s) 2852
PinAI ACCGGT 1 cut(s) 1816
PleI GAGTC 4 cut(s) 169, 551, 2080, 2414
PluTI GGCGCC 1 cut(s) 57
PpsI GAGTC 4 cut(s) 169, 551, 2080, 2414
Ppu21I YACGTR 1 cut(s) 3127
PpuMI RGGWCCY 1 cut(s) 713
PscI ACATGT 3 cut(s) 357, 864, 2949
PshAI GACNNNNGTC 1 cut(s) 152
PshBI ATTAAT 1 cut(s) 21
Psp124BI GAGCTC 4 cut(s) 549, 607, 1530, 2861
Psp5II RGGWCCY 1 cut(s) 713
Psp6I CCWGG 5 cut(s) 224, 619, 1282, 2563, 2852
PspEI GGTNACC 1 cut(s) 2180
PspGI CCWGG 5 cut(s) 224, 619, 1282, 2563, 2852
PspPI GGNCC 8 cut(s) 166, 526, 713, 779, 1514, 1814, 1858, 2243
PspPPI RGGWCCY 1 cut(s) 713
PstI CTGCAG 3 cut(s) 712, 1129, 2173
PstNI CAGNNNCTG 3 cut(s) 716, 2177, 2480
PsuI RGATCY 6 cut(s) 637, 1465, 2153, 2362, 2602, 2773
RsaI GTAC 8 cut(s) 29, 128, 810, 1147, 1363, 2569, 2849, 2915
RsaNI GTAC 8 cut(s) 28, 127, 809, 1146, 1362, 2568, 2848, 2914
RseI CAYNNNNRTG 1 cut(s) 2954
SacI GAGCTC 4 cut(s) 549, 607, 1530, 2861
SapI GCTCTTC 3 cut(s) 966, 1158, 3048
Sau96I GGNCC 8 cut(s) 166, 526, 713, 779, 1514, 1814, 1858, 2243
ScaI AGTACT 2 cut(s) 128, 810
SchI GAGTC 4 cut(s) 170, 551, 2081, 2414
ScrFI CCNGG 6 cut(s) 226, 530, 621, 1284, 2565, 2854
SfcI CTRYAG 4 cut(s) 708, 1125, 2169, 2481
SfoI GGCGCC 1 cut(s) 55
Sfr274I CTCGAG 2 cut(s) 230, 2895
SfuI TTCGAA 1 cut(s) 452
SinI GGWCC 8 cut(s) 166, 526, 713, 779, 1514, 1814, 1858, 2243
SlaI CTCGAG 2 cut(s) 230, 2895
SmiMI CAYNNNNRTG 1 cut(s) 2954
SmlI CTYRAG 6 cut(s) 230, 1037, 1202, 1895, 2090, 2895
SmoI CTYRAG 6 cut(s) 230, 1037, 1202, 1895, 2090, 2895
SnaBI TACGTA 1 cut(s) 3127
SpeI ACTAGT 1 cut(s) 1132
SspDI GGCGCC 1 cut(s) 53
SspI AATATT 1 cut(s) 448
SstI GAGCTC 4 cut(s) 549, 607, 1530, 2861
StyD4I CCNGG 6 cut(s) 224, 528, 619, 1282, 2563, 2852
StyI CCWWGG 4 cut(s) 425, 1246, 1262, 2962
TaaI ACNGT 6 cut(s) 279, 1609, 1697, 1811, 2482, 2633
TaiI ACGT 5 cut(s) 182, 340, 2193, 2212, 3129
TaqI TCGA 6 cut(s) 156, 231, 452, 553, 1877, 2896
TaqII GACCGA 1 cut(s) 539
TatI WGTACW 4 cut(s) 126, 808, 1145, 2847
TauI GCSGC 3 cut(s) 54, 2016, 2586
TfiI GAWTC 5 cut(s) 91, 768, 2084, 2237, 2380
TscAI CASTG 4 cut(s) 754, 1548, 2182, 2548
TseFI GTSAC 4 cut(s) 145, 2164, 2180, 2437
Tsp45I GTSAC 4 cut(s) 145, 2164, 2180, 2437
TspDTI ATGAA 7 cut(s) 914, 1714, 2133, 2387, 2755, 3003, 3041
TspGWI ACGGA 1 cut(s) 2332
TspRI CASTG 4 cut(s) 754, 1548, 2182, 2548
Vha464I CTTAAG 3 cut(s) 1037, 1895, 2090
VneI GTGCAC 1 cut(s) 1887
VpaK11BI GGWCC 8 cut(s) 166, 526, 713, 779, 1514, 1814, 1858, 2243
VspI ATTAAT 1 cut(s) 21
XapI RAATTY 4 cut(s) 830, 1915, 2519, 2698
XceI RCATGY 4 cut(s) 361, 868, 1261, 2953
XcmI CCANNNNNNNNNTGG 2 cut(s) 130, 1355
XhoI CTCGAG 2 cut(s) 230, 2895
XmiI GTMKAC 1 cut(s) 1580
XmnI GAANNNNTTC 3 cut(s) 434, 1032, 2593
ZrmI AGTACT 2 cut(s) 128, 810
Zsp2I ATGCAT 1 cut(s) 2617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.