pycom04g01670

Transcription factor IIIC subunit delta N-term

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0009196
Physical Location & Seq
Reverse (-)
1583306 .. 1586503
3198 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1374 bp
ATGTCTAGCCAAAAATTTGACAAGATTGGCACATATGATGCACATGGCCAGACGGTTACAGGTTTAGCTTGGGCGTTTGATGGGCAATCTTTGTATAGCTGCAGCCAGGATAATGTTATACGCTGTTGGATTTTGAGTGGAAGTTCTCTCTGTGAAGTACCTATTCCTTCAAATACTCCTCGTCTGAGAAACTCAACTGATTTACCAGAAGAATTTGTTTCTTGCTTTGGTCTGGCAGTGTCCCCTGGAAATCTTGTGATTGCTTGGGTTCGAAATACTGATGTTGAACAATTAAACCCAATGTACGAGGCAAGGACTCAAAAGGCCATTGTTGAATTTTTCTGGACCGGTGGTCAGCAAGTAGATGTCTTCTCAAAAAATTCCGTACATTTTGATACTGAAGCGATTCCTGGTTTTTCTGAGGAGTTAGTTTATTGGGAGTCCAACTTTCAATGGTCCTTAAAGCAGTATGAAATGCAAGATAAACATCTGGTTGTTTGGGATATTGTAACAGCATTGTTGGCTTTTAATCATTCTAAACCAGAGTTTGTAGAACAAGTACTGATTAAATGGCTCTCTATTTCATACTTGGGATCTCATGCGGGAATTCCTGCTGAAACGGTTTTGTTGAATGTCTCCCAAAGTTGCTCAAAAGTCACTTCTCGCCAATTACATCTTCTCAATATAATCTGCAGGCGTGTGATTCTATCAGAGATGAAGGCTGATGAAATAAACAGTAAATTACTGAATTTGAAAGGAATTCATGGTGCTGAAAAAGAGAAGCGGCCTTTGTGGATCAATCTGCTTGTGAACAGTGAGAAAGAACTCCGGGAAAGGCTTGTGGGATTTACATTTTCTGCTCTTTTAAGTCAAATGCCTGCTTCAGCCCCAGATTCTCCATCTAGAAACTGGTTTCCTATTGGATTAGCACAAATGGAGCAGTGGATTGAGCTTAATCATGACCATGTGCACAGTCAGCTAAAGGTCCTCGCATCCGAAGTTGGAAAGCGGGATAGGAGATTTGGATCCAGTAAATGCATAGCAGCAGAGATGTGCAGCTATTGTTTAGCATCAGTTCCATTTGAATCTCCAGAAGTTGCCTTCTGCAGCGGAAAGGGCCACAAACTAGTTAGGTGTTCCATTTCTATGGAGATTTGTCCTACTACTCCGACATGGTTTTGCACATGCTGTCATAGACGAGCTTCCAAACTGGCACCAGCGACTCTGTTTACAACCCCCGGTCTTCCTTTGAATTTCAATTCTTTGACCGCATCGCCTTTGCTAGAAGTATCCTTTAAACCACTCTGTCCATTTTGTGGTATACTGCTACAAAGACTACAACCGGATTTCCTACTTTCTGCATCACCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TFIIIC_delta WD40 zf-TFIIIC

Protein Analysis

458

Amino Acids

51.19

Weight (kDa)

6.47

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40 PF00400 8 - 43 6.7e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1213
AccB7I CCANNNNNTGG 1 cut(s) 1316
AccI GTMKAC 1 cut(s) 1321
AciI CCGC 5 cut(s) 602, 784, 1009, 1110, 1269
AclWI GGATC 4 cut(s) 601, 803, 1020, 1033
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 8 cut(s) 14, 212, 335, 379, 606, 748, 759, 1252
AcuI CTGAAG 2 cut(s) 420, 867
AfaI GTAC 4 cut(s) 159, 305, 387, 561
AfiI CCNNNNNNNGG 1 cut(s) 1316
AgeI ACCGGT 1 cut(s) 347
AgsI TTSAA 9 cut(s) 171, 287, 335, 452, 631, 754, 1085, 1252, 1258
AhdI GACNNNNNGTC 1 cut(s) 351
AhlI ACTAGT 1 cut(s) 1126
AjnI CCWGG 3 cut(s) 105, 244, 409
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AluBI AGCT 6 cut(s) 68, 99, 952, 979, 1059, 1202
AluI AGCT 6 cut(s) 68, 99, 952, 979, 1059, 1202
Alw21I GWGCWC 1 cut(s) 972
Alw26I GTCTC 1 cut(s) 640
Alw44I GTGCAC 1 cut(s) 968
AlwI GGATC 4 cut(s) 601, 803, 1020, 1033
AoxI GGCC 4 cut(s) 46, 324, 785, 1117
ApaLI GTGCAC 1 cut(s) 968
ApeKI GCWGC 5 cut(s) 99, 102, 1043, 1056, 1107
ApoI RAATTY 8 cut(s) 14, 212, 335, 379, 606, 748, 759, 1252
AsiGI ACCGGT 1 cut(s) 347
Asp700I GAANNNNTTC 1 cut(s) 405
AspS9I GGNCC 4 cut(s) 345, 456, 985, 1117
AsuC2I CCSGG 2 cut(s) 830, 1239
AsuHPI GGTGA 1 cut(s) 1356
AsuII TTCGAA 1 cut(s) 271
AvaII GGWCC 3 cut(s) 345, 456, 985
BaeGI GKGCMC 1 cut(s) 972
BalI TGGCCA 1 cut(s) 48
BamHI GGATCC 1 cut(s) 1025
BanI GGYRCC 1 cut(s) 1213
BbsI GAAGAC 2 cut(s) 361, 1235
Bbv12I GWGCWC 1 cut(s) 972
BbvI GCAGC 5 cut(s) 86, 114, 1055, 1068, 1119
BccI CCATC 2 cut(s) 74, 907
BciT130I CCWGG 3 cut(s) 107, 246, 411
BciVI GTATCC 1 cut(s) 1300
BcnI CCSGG 2 cut(s) 830, 1239
BcoDI GTCTC 1 cut(s) 640
BcuI ACTAGT 1 cut(s) 1126
BfaI CTAG 4 cut(s) 6, 903, 1127, 1283
BfmI CTRYAG 3 cut(s) 100, 691, 1105
BfuI GTATCC 1 cut(s) 1300
BisI GCNGC 6 cut(s) 100, 103, 785, 1044, 1057, 1108
BlsI GCNGC 6 cut(s) 101, 104, 786, 1045, 1058, 1109
BmcAI AGTACT 1 cut(s) 561
Bme1390I CCNGG 5 cut(s) 107, 246, 411, 830, 1239
Bme18I GGWCC 3 cut(s) 345, 456, 985
BmeRI GACNNNNNGTC 1 cut(s) 351
BmgT120I GGNCC 4 cut(s) 345, 456, 985, 1117
BmiI GGNNCC 2 cut(s) 1027, 1215
BmrFI CCNGG 5 cut(s) 107, 246, 411, 830, 1239
BmsI GCATC 5 cut(s) 28, 1001, 1079, 1280, 1370
BpiI GAAGAC 2 cut(s) 361, 1235
BpmI CTGGAG 1 cut(s) 1074
Bpu14I TTCGAA 1 cut(s) 271
BpuMI CCSGG 2 cut(s) 830, 1239
BsaBI GATNNNNATC 2 cut(s) 486, 1024
BsaJI CCNNGG 2 cut(s) 244, 1237
BsaWI WCCGGW 2 cut(s) 347, 1342
Bsc4I CCNNNNNNNGG 1 cut(s) 1316
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 3 cut(s) 914, 1029, 1215
Bse8I GATNNNNATC 2 cut(s) 486, 1024
BseBI CCWGG 3 cut(s) 107, 246, 411
BseDI CCNNGG 2 cut(s) 244, 1237
BseGI GGATG 1 cut(s) 992
BseJI GATNNNNATC 2 cut(s) 486, 1024
BseLI CCNNNNNNNGG 1 cut(s) 1316
BseMII CTCAG 2 cut(s) 176, 411
BseNI ACTGG 3 cut(s) 914, 1029, 1215
BseRI GAGGAG 2 cut(s) 168, 437
BseSI GKGCMC 1 cut(s) 972
BseXI GCAGC 5 cut(s) 86, 114, 1055, 1068, 1119
BsgI GTGCAG 1 cut(s) 1075
BshFI GGCC 4 cut(s) 48, 326, 787, 1119
BshNI GGYRCC 1 cut(s) 1213
BshTI ACCGGT 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 972
BsiSI CCGG 4 cut(s) 348, 829, 1239, 1343
BslFI GGGAC 1 cut(s) 226
BslI CCNNNNNNNGG 1 cut(s) 1316
BsmAI GTCTC 1 cut(s) 640
BsmFI GGGAC 1 cut(s) 226
BsnI GGCC 4 cut(s) 48, 326, 787, 1119
Bsp119I TTCGAA 1 cut(s) 271
Bsp1286I GDGCHC 1 cut(s) 972
Bsp143I GATC 3 cut(s) 593, 795, 1025
BspACI CCGC 5 cut(s) 602, 784, 1009, 1110, 1269
BspANI GGCC 4 cut(s) 48, 326, 787, 1119
BspCNI CTCAG 2 cut(s) 177, 412
BspHI TCATGA 1 cut(s) 958
BspLI GGNNCC 2 cut(s) 1027, 1215
BspMAI CTGCAG 3 cut(s) 104, 695, 1109
BspPI GGATC 4 cut(s) 601, 803, 1020, 1033
BspT104I TTCGAA 1 cut(s) 271
BspT107I GGYRCC 1 cut(s) 1213
BsrFI RCCGGY 1 cut(s) 347
BsrI ACTGG 3 cut(s) 914, 1029, 1215
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 2 cut(s) 244, 1237
BssMI GATC 3 cut(s) 593, 795, 1025
BssNAI GTATAC 1 cut(s) 1322
Bst1107I GTATAC 1 cut(s) 1322
Bst2UI CCWGG 3 cut(s) 107, 246, 411
Bst4CI ACNGT 5 cut(s) 55, 622, 737, 815, 974
BstBI TTCGAA 1 cut(s) 271
BstC8I GCNNGC 2 cut(s) 695, 879
BstDEI CTNAG 2 cut(s) 185, 420
BstF5I GGATG 1 cut(s) 992
BstKTI GATC 3 cut(s) 596, 798, 1028
BstMAI GTCTC 1 cut(s) 640
BstMBI GATC 3 cut(s) 593, 795, 1025
BstMWI GCNNNNNNNGC 3 cut(s) 521, 976, 1116
BstNI CCWGG 3 cut(s) 107, 246, 411
BstNSI RCATGY 1 cut(s) 1188
BstSCI CCNGG 5 cut(s) 105, 244, 409, 828, 1237
BstSFI CTRYAG 3 cut(s) 100, 691, 1105
BstSLI GKGCMC 1 cut(s) 972
BstV1I GCAGC 5 cut(s) 86, 114, 1055, 1068, 1119
BstV2I GAAGAC 2 cut(s) 361, 1235
BstX2I RGATCY 2 cut(s) 593, 1025
BstXI CCANNNNNNTGG 1 cut(s) 1147
BstYI RGATCY 2 cut(s) 593, 1025
BstZ17I GTATAC 1 cut(s) 1322
BsuI GTATCC 1 cut(s) 1300
BsuRI GGCC 4 cut(s) 48, 326, 787, 1119
BtgZI GCGATG 1 cut(s) 1257
BtsCI GGATG 1 cut(s) 992
BtsI GCAGTG 2 cut(s) 243, 947
BtsIMutI CAGTG 3 cut(s) 243, 820, 947
Cac8I GCNNGC 2 cut(s) 695, 879
CciI TCATGA 1 cut(s) 958
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 4 cut(s) 345, 456, 985, 1117
Csp6I GTAC 4 cut(s) 158, 304, 386, 560
CspAI ACCGGT 1 cut(s) 347
CviAII CATG 7 cut(s) 44, 599, 764, 959, 965, 1173, 1185
CviQI GTAC 4 cut(s) 158, 304, 386, 560
DdeI CTNAG 2 cut(s) 185, 420
DpnI GATC 3 cut(s) 595, 797, 1027
DpnII GATC 3 cut(s) 593, 795, 1025
DraI TTTAAA 1 cut(s) 1297
DriI GACNNNNNGTC 1 cut(s) 351
EaeI YGGCCR 1 cut(s) 46
Eam1105I GACNNNNNGTC 1 cut(s) 351
Eco47I GGWCC 3 cut(s) 345, 456, 985
Eco57I CTGAAG 2 cut(s) 420, 867
EcoO109I RGGNCCY 1 cut(s) 985
EcoRI GAATTC 2 cut(s) 606, 759
EcoRII CCWGG 3 cut(s) 105, 244, 409
EcoT22I ATGCAT 1 cut(s) 1040
FaeI CATG 7 cut(s) 47, 602, 767, 962, 968, 1176, 1188
FaqI GGGAC 1 cut(s) 226
FatI CATG 7 cut(s) 43, 598, 763, 958, 964, 1172, 1184
FauI CCCGC 2 cut(s) 595, 1002
FauNDI CATATG 1 cut(s) 34
FblI GTMKAC 1 cut(s) 1321
Fnu4HI GCNGC 6 cut(s) 100, 103, 785, 1044, 1057, 1108
FokI GGATG 1 cut(s) 979
Fsp4HI GCNGC 6 cut(s) 100, 103, 785, 1044, 1057, 1108
FspBI CTAG 4 cut(s) 6, 903, 1127, 1283
GluI GCNGC 6 cut(s) 100, 103, 785, 1044, 1057, 1108
GsuI CTGGAG 1 cut(s) 1074
HaeIII GGCC 4 cut(s) 48, 326, 787, 1119
HapII CCGG 4 cut(s) 348, 829, 1239, 1343
Hin1II CATG 7 cut(s) 47, 602, 767, 962, 968, 1176, 1188
HinfI GANTC 7 cut(s) 316, 406, 440, 703, 893, 1085, 1222
HpaII CCGG 4 cut(s) 348, 829, 1239, 1343
HphI GGTGA 1 cut(s) 1356
Hpy166II GTNNAC 4 cut(s) 811, 970, 1230, 1322
Hpy188I TCNGA 5 cut(s) 186, 421, 712, 997, 1170
Hpy188III TCNNGA 4 cut(s) 343, 903, 959, 1091
Hpy8I GTNNAC 4 cut(s) 811, 970, 1230, 1322
HpyAV CCTTC 3 cut(s) 177, 712, 1111
HpyCH4III ACNGT 5 cut(s) 55, 622, 737, 815, 974
HpyF10VI GCNNNNNNNGC 3 cut(s) 521, 976, 1116
HpyF3I CTNAG 2 cut(s) 185, 420
Hsp92II CATG 7 cut(s) 47, 602, 767, 962, 968, 1176, 1188
Kzo9I GATC 3 cut(s) 593, 795, 1025
LmnI GCTCC 1 cut(s) 937
Lsp1109I GCAGC 5 cut(s) 86, 114, 1055, 1068, 1119
LweI GCATC 5 cut(s) 28, 1001, 1079, 1280, 1370
MaeI CTAG 4 cut(s) 6, 903, 1127, 1283
MaeIII GTNAC 3 cut(s) 55, 508, 655
MalI GATC 3 cut(s) 595, 797, 1027
MboI GATC 3 cut(s) 593, 795, 1025
MboII GAAGA 4 cut(s) 221, 361, 668, 1235
MflI RGATCY 2 cut(s) 593, 1025
MhlI GDGCHC 1 cut(s) 972
MlsI TGGCCA 1 cut(s) 48
MluNI TGGCCA 1 cut(s) 48
MlyI GAGTC 3 cut(s) 310, 449, 1216
MmeI TCCRAC 4 cut(s) 107, 468, 982, 1193
MnlI CCTC 4 cut(s) 189, 301, 415, 998
Mox20I TGGCCA 1 cut(s) 48
Mph1103I ATGCAT 1 cut(s) 1040
MroXI GAANNNNTTC 1 cut(s) 405
MscI TGGCCA 1 cut(s) 48
MseI TTAA 8 cut(s) 293, 461, 528, 567, 866, 954, 1296, 1372
MslI CAYNNNNRTG 2 cut(s) 963, 1145
Msp20I TGGCCA 1 cut(s) 48
MspA1I CMGCKG 1 cut(s) 1110
MspI CCGG 4 cut(s) 348, 829, 1239, 1343
MspR9I CCNGG 5 cut(s) 107, 246, 411, 830, 1239
MvaI CCWGG 3 cut(s) 107, 246, 411
MwoI GCNNNNNNNGC 3 cut(s) 521, 976, 1116
NciI CCSGG 2 cut(s) 830, 1239
NdeI CATATG 1 cut(s) 34
NdeII GATC 3 cut(s) 593, 795, 1025
NlaIII CATG 7 cut(s) 47, 602, 767, 962, 968, 1176, 1188
NlaIV GGNNCC 2 cut(s) 1027, 1215
NmuCI GTSAC 1 cut(s) 655
NsiI ATGCAT 1 cut(s) 1040
NspI RCATGY 1 cut(s) 1188
NspV TTCGAA 1 cut(s) 271
PagI TCATGA 1 cut(s) 958
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 4 cut(s) 406, 703, 893, 1085
PflMI CCANNNNNTGG 1 cut(s) 1316
PfoI TCCNGGA 1 cut(s) 828
PinAI ACCGGT 1 cut(s) 347
PkrI GCNGC 6 cut(s) 101, 104, 786, 1045, 1058, 1109
PleI GAGTC 3 cut(s) 310, 448, 1216
PpsI GAGTC 3 cut(s) 310, 448, 1216
PpuMI RGGWCCY 1 cut(s) 985
Psp5II RGGWCCY 1 cut(s) 985
Psp6I CCWGG 3 cut(s) 105, 244, 409
PspGI CCWGG 3 cut(s) 105, 244, 409
PspN4I GGNNCC 2 cut(s) 1027, 1215
PspPI GGNCC 4 cut(s) 345, 456, 985, 1117
PspPPI RGGWCCY 1 cut(s) 985
PstI CTGCAG 3 cut(s) 104, 695, 1109
PsuI RGATCY 2 cut(s) 593, 1025
RsaI GTAC 4 cut(s) 159, 305, 387, 561
RsaNI GTAC 4 cut(s) 158, 304, 386, 560
RseI CAYNNNNRTG 2 cut(s) 963, 1145
SaqAI TTAA 8 cut(s) 293, 461, 528, 567, 866, 954, 1296, 1372
SatI GCNGC 6 cut(s) 100, 103, 785, 1044, 1057, 1108
Sau3AI GATC 3 cut(s) 593, 795, 1025
Sau96I GGNCC 4 cut(s) 345, 456, 985, 1117
ScaI AGTACT 1 cut(s) 561
SchI GAGTC 3 cut(s) 310, 449, 1216
ScrFI CCNGG 5 cut(s) 107, 246, 411, 830, 1239
SduI GDGCHC 1 cut(s) 972
SfaNI GCATC 5 cut(s) 28, 1001, 1079, 1280, 1370
SfcI CTRYAG 3 cut(s) 100, 691, 1105
SfuI TTCGAA 1 cut(s) 271
SinI GGWCC 3 cut(s) 345, 456, 985
SmiMI CAYNNNNRTG 2 cut(s) 963, 1145
SpeI ACTAGT 1 cut(s) 1126
SsiI CCGC 5 cut(s) 602, 784, 1009, 1110, 1269
SspMI CTAG 4 cut(s) 6, 903, 1127, 1283
StyD4I CCNGG 5 cut(s) 105, 244, 409, 828, 1237
TaaI ACNGT 5 cut(s) 55, 622, 737, 815, 974
TaqI TCGA 1 cut(s) 271
TatI WGTACW 1 cut(s) 559
TauI GCSGC 1 cut(s) 787
TfiI GAWTC 4 cut(s) 406, 703, 893, 1085
Tru1I TTAA 8 cut(s) 293, 461, 528, 567, 866, 954, 1296, 1372
Tru9I TTAA 8 cut(s) 293, 461, 528, 567, 866, 954, 1296, 1372
TscAI CASTG 3 cut(s) 243, 820, 947
TseFI GTSAC 1 cut(s) 655
TseI GCWGC 5 cut(s) 99, 102, 1043, 1056, 1107
Tsp45I GTSAC 1 cut(s) 655
TspDTI ATGAA 5 cut(s) 486, 573, 731, 741, 752
TspGWI ACGGA 1 cut(s) 373
TspRI CASTG 3 cut(s) 243, 820, 947
Van91I CCANNNNNTGG 1 cut(s) 1316
VneI GTGCAC 1 cut(s) 968
VpaK11BI GGWCC 3 cut(s) 345, 456, 985
XapI RAATTY 8 cut(s) 14, 212, 335, 379, 606, 748, 759, 1252
XbaI TCTAGA 1 cut(s) 902
XceI RCATGY 1 cut(s) 1188
XcmI CCANNNNNNNNNTGG 1 cut(s) 906
XmiI GTMKAC 1 cut(s) 1321
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 4 cut(s) 6, 903, 1127, 1283
ZrmI AGTACT 1 cut(s) 561
Zsp2I ATGCAT 1 cut(s) 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.