pycom04g02450

CRIB domain-containing protein RIC4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0012882
Physical Location & Seq
Forward (+)
2176321 .. 2178065
1745 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g02450.2

Sequence Viewer

Length: 639 bp
ATGGTTTGTAAGTCCCGCAACAAAACAAAAAAGCTTAGGCTTTGTATTTTTGTGTCCGAAGGGATGAGGGATCGAATGGAGAGATTTGCTGTTCTTCCTTTTGGCATTGGCTGCGCTTCTCAGTCCAGCGTTGCTATTGGCGCAGCAGCAAGTGCTGATCATCAACAAAGAAAATCTAAAGCACCACCTCCACATACAGCAAATGATCCAAAAGCACAAGGAGAAAAAATGAAGAGGGGTACATTTCACTTCCTGACTCTTAAGAGGACTCAAATATCCAGTGGGATGCAGAGATTGATCAGAAGCATAAAAAGCTTCTCCCAAATATTCGTTTACAAGGAAGAGATTGAGGAAGAGGATGAGGAAGGAGAGATGGAAATTGGATTGCCGACGGACGTGAAGCATGTGACACACATAGGACTGGATGGGTCTGCCACTACCAATACCACCGCAAACCCCTTAAAAGCTAGCAGTTGGGAAAATAATTTCAACACTCCAGAAATACTTTCTTTCCCTTCAATTTCTTTAAAGCAGTTTGAGCTTGCCATGGCTGCACAGACCACCCACCAACCTGAGCAGCAGCAGCAGCAGCTCGTTGTTGATCTCAAGACGCTGGAAGATCCCAAAGATCTTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD

Protein Analysis

213

Amino Acids

23.65

Weight (kDa)

7.75

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27380 AT1G27380 AT1G27380 AT1G27380
fragaria_vesca FvH4_4g12280
malus_domestica MD04G1027900.v1.1
prunus_persica Prupe.1G177300_v2.0.a1
pyrus_communis pycom04g02450
rosa_chinensis RchiOBHm_Chr4g0410351
rosa_laevigata RLG00000008435
rosa_roxburghii Rroxscaffold_5G00355150
rosa_rugosa Rorug04G0102200 Rorug04G0102200
rosa_samantha Rh4AG160200 Rh4BG159100 Rh4CG172300 Rh4DG155100
rosa_wichuraiana Rw4G013340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 16, 450
AclWI GGATC 3 cut(s) 78, 200, 614
AfaI GTAC 1 cut(s) 241
AflII CTTAAG 1 cut(s) 260
AgsI TTSAA 2 cut(s) 490, 519
AjiI CACGTC 1 cut(s) 397
AluBI AGCT 6 cut(s) 34, 315, 467, 541, 592, 636
AluI AGCT 6 cut(s) 34, 315, 467, 541, 592, 636
AlwI GGATC 3 cut(s) 78, 200, 614
ApeKI GCWGC 9 cut(s) 111, 143, 146, 551, 577, 580, 583, 586, 589
AspLEI GCGC 2 cut(s) 116, 143
AsuNHI GCTAGC 1 cut(s) 467
BbvI GCAGC 9 cut(s) 98, 155, 158, 538, 589, 592, 595, 598, 601
BccI CCATC 2 cut(s) 367, 419
BclI TGATCA 2 cut(s) 157, 297
BfaI CTAG 2 cut(s) 468, 637
BfrI CTTAAG 1 cut(s) 260
BglII AGATCT 1 cut(s) 628
BisI GCNGC 9 cut(s) 112, 144, 147, 552, 578, 581, 584, 587, 590
BlsI GCNGC 9 cut(s) 113, 145, 148, 553, 579, 582, 585, 588, 591
BmgBI CACGTC 1 cut(s) 397
BmsI GCATC 1 cut(s) 276
BmtI GCTAGC 1 cut(s) 471
BpmI CTGGAG 1 cut(s) 480
Bpu10I CCTNAGC 2 cut(s) 35, 573
BpuEI CTTGAG 1 cut(s) 590
BsaJI CCNNGG 1 cut(s) 546
Bse1I ACTGG 2 cut(s) 279, 426
BseDI CCNNGG 1 cut(s) 546
BseGI GGATG 4 cut(s) 69, 291, 364, 430
BseMII CTCAG 2 cut(s) 134, 564
BseNI ACTGG 2 cut(s) 279, 426
BseXI GCAGC 9 cut(s) 98, 155, 158, 538, 589, 592, 595, 598, 601
BsgI GTGCAG 1 cut(s) 537
Bsp143I GATC 7 cut(s) 70, 157, 205, 297, 601, 619, 628
Bsp19I CCATGG 1 cut(s) 546
BspACI CCGC 2 cut(s) 16, 450
BspCNI CTCAG 2 cut(s) 133, 565
BspOI GCTAGC 1 cut(s) 471
BspPI GGATC 3 cut(s) 78, 200, 614
BspTI CTTAAG 1 cut(s) 260
BsrI ACTGG 2 cut(s) 279, 426
BssECI CCNNGG 1 cut(s) 546
BssMI GATC 7 cut(s) 70, 157, 205, 297, 601, 619, 628
BssT1I CCWWGG 1 cut(s) 546
Bst6I CTCTTC 3 cut(s) 227, 336, 348
BstAFI CTTAAG 1 cut(s) 260
BstAPI GCANNNNNTGC 2 cut(s) 111, 152
BstC8I GCNNGC 2 cut(s) 469, 543
BstDEI CTNAG 4 cut(s) 35, 120, 573, 632
BstDSI CCRYGG 1 cut(s) 546
BstF5I GGATG 4 cut(s) 69, 291, 364, 430
BstHHI GCGC 2 cut(s) 116, 143
BstKTI GATC 7 cut(s) 73, 160, 208, 300, 604, 622, 631
BstMBI GATC 7 cut(s) 70, 157, 205, 297, 601, 619, 628
BstMWI GCNNNNNNNGC 9 cut(s) 111, 140, 152, 312, 538, 551, 583, 586, 589
BstNSI RCATGY 1 cut(s) 407
BstV1I GCAGC 9 cut(s) 98, 155, 158, 538, 589, 592, 595, 598, 601
BstX2I RGATCY 2 cut(s) 619, 628
BstYI RGATCY 2 cut(s) 619, 628
BtgI CCRYGG 1 cut(s) 546
BtrI CACGTC 1 cut(s) 397
BtsCI GGATG 4 cut(s) 69, 291, 364, 430
BtsIMutI CAGTG 1 cut(s) 286
Cac8I GCNNGC 2 cut(s) 469, 543
CfoI GCGC 2 cut(s) 116, 143
CseI GACGC 1 cut(s) 619
Csp6I GTAC 1 cut(s) 240
CviAII CATG 2 cut(s) 404, 547
CviJI RGCY 9 cut(s) 34, 40, 111, 315, 467, 541, 551, 592, 636
CviKI_1 RGCY 9 cut(s) 34, 40, 111, 315, 467, 541, 551, 592, 636
CviQI GTAC 1 cut(s) 240
DdeI CTNAG 4 cut(s) 35, 120, 573, 632
DpnI GATC 7 cut(s) 72, 159, 207, 299, 603, 621, 630
DpnII GATC 7 cut(s) 70, 157, 205, 297, 601, 619, 628
DraI TTTAAA 1 cut(s) 528
Eam1104I CTCTTC 3 cut(s) 227, 336, 348
EarI CTCTTC 3 cut(s) 227, 336, 348
Eco130I CCWWGG 1 cut(s) 546
EcoT14I CCWWGG 1 cut(s) 546
ErhI CCWWGG 1 cut(s) 546
FaeI CATG 2 cut(s) 407, 550
FaiI YATR 5 cut(s) 195, 308, 405, 416, 548
FatI CATG 2 cut(s) 403, 546
FauI CCCGC 1 cut(s) 23
FbaI TGATCA 2 cut(s) 157, 297
Fnu4HI GCNGC 9 cut(s) 112, 144, 147, 552, 578, 581, 584, 587, 590
FokI GGATG 4 cut(s) 76, 298, 371, 437
Fsp4HI GCNGC 9 cut(s) 112, 144, 147, 552, 578, 581, 584, 587, 590
FspBI CTAG 2 cut(s) 468, 637
GlaI GCGC 2 cut(s) 115, 142
GluI GCNGC 9 cut(s) 112, 144, 147, 552, 578, 581, 584, 587, 590
GsuI CTGGAG 1 cut(s) 480
HgaI GACGC 1 cut(s) 619
HhaI GCGC 2 cut(s) 116, 143
Hin1II CATG 2 cut(s) 407, 550
Hin6I GCGC 2 cut(s) 114, 141
HinP1I GCGC 2 cut(s) 114, 141
HindIII AAGCTT 2 cut(s) 32, 313
HinfI GANTC 2 cut(s) 256, 268
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 2 cut(s) 58, 302
Hpy188III TCNNGA 3 cut(s) 253, 497, 607
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 1 cut(s) 394
HpyAV CCTTC 3 cut(s) 53, 359, 525
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 2 cut(s) 289, 554
HpyF10VI GCNNNNNNNGC 9 cut(s) 111, 140, 152, 312, 538, 551, 583, 586, 589
HpyF3I CTNAG 4 cut(s) 35, 120, 573, 632
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 2 cut(s) 407, 550
HspAI GCGC 2 cut(s) 114, 141
Ksp22I TGATCA 2 cut(s) 157, 297
Kzo9I GATC 7 cut(s) 70, 157, 205, 297, 601, 619, 628
LpnPI CCDG 7 cut(s) 139, 266, 292, 407, 510, 585, 599
Lsp1109I GCAGC 9 cut(s) 98, 155, 158, 538, 589, 592, 595, 598, 601
LweI GCATC 1 cut(s) 276
MaeI CTAG 2 cut(s) 468, 637
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 1 cut(s) 406
MalI GATC 7 cut(s) 72, 159, 207, 299, 603, 621, 630
MboI GATC 7 cut(s) 70, 157, 205, 297, 601, 619, 628
MboII GAAGA 5 cut(s) 86, 244, 353, 365, 629
MflI RGATCY 2 cut(s) 619, 628
MluCI AATT 3 cut(s) 378, 484, 519
MlyI GAGTC 2 cut(s) 250, 262
MnlI CCTC 7 cut(s) 60, 198, 228, 258, 343, 349, 355
MseI TTAA 3 cut(s) 261, 461, 527
MspCI CTTAAG 1 cut(s) 260
MwoI GCNNNNNNNGC 9 cut(s) 111, 140, 152, 312, 538, 551, 583, 586, 589
NcoI CCATGG 1 cut(s) 546
NdeII GATC 7 cut(s) 70, 157, 205, 297, 601, 619, 628
NheI GCTAGC 1 cut(s) 467
NlaIII CATG 2 cut(s) 407, 550
NmuCI GTSAC 1 cut(s) 406
NspI RCATGY 1 cut(s) 407
PkrI GCNGC 9 cut(s) 113, 145, 148, 553, 579, 582, 585, 588, 591
PleI GAGTC 2 cut(s) 250, 262
PpsI GAGTC 2 cut(s) 250, 262
PsuI RGATCY 2 cut(s) 619, 628
RsaI GTAC 1 cut(s) 241
RsaNI GTAC 1 cut(s) 240
SaqAI TTAA 3 cut(s) 261, 461, 527
SatI GCNGC 9 cut(s) 112, 144, 147, 552, 578, 581, 584, 587, 590
Sau3AI GATC 7 cut(s) 70, 157, 205, 297, 601, 619, 628
SchI GAGTC 2 cut(s) 250, 262
SetI ASST 9 cut(s) 36, 190, 317, 399, 469, 543, 574, 594, 638
SfaNI GCATC 1 cut(s) 276
SmlI CTYRAG 2 cut(s) 260, 605
SmoI CTYRAG 2 cut(s) 260, 605
Sse9I AATT 3 cut(s) 378, 484, 519
SsiI CCGC 2 cut(s) 16, 450
SspI AATATT 1 cut(s) 327
SspMI CTAG 2 cut(s) 468, 637
StyI CCWWGG 1 cut(s) 546
TaiI ACGT 1 cut(s) 399
TaqI TCGA 1 cut(s) 73
TasI AATT 3 cut(s) 378, 484, 519
Tru1I TTAA 3 cut(s) 261, 461, 527
Tru9I TTAA 3 cut(s) 261, 461, 527
TscAI CASTG 1 cut(s) 286
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 9 cut(s) 111, 143, 146, 551, 577, 580, 583, 586, 589
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 1 cut(s) 245
TspGWI ACGGA 1 cut(s) 407
TspRI CASTG 1 cut(s) 286
Vha464I CTTAAG 1 cut(s) 260
XceI RCATGY 1 cut(s) 407
XspI CTAG 2 cut(s) 468, 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.