pycom04g05840

bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0012053
Physical Location & Seq
Forward (+)
5688184 .. 5690867
2684 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1053 bp
ATGGGGTCTATGGAGCAGACGATTCTGGTGACCGGCGGCGCGGGGTTCATAGGGAGCCACACGGTGGTTCAGCTGCTCGAGGCGGGGTTTAGGGTTTCCGTCATTGATAATCTCGATAACTCCGTTGCTGAAGCTGTTGATCGCGTCAGGGACTTGGTCGGCCCCAAGCTCTCTCAGAAACTTCACTTTCAGCTGGGTGATCTCAGAAATAAGGACGATTTGGAGAAGCTGTTTTCTCAAACGAAGTTTGATGCTGTAATCCACTTTGCTGGCCTAAAAGCTGTAGGAGAAAGCGTTGCACACCCGCACCGTTATTATGATAACAATTTGGTTGGAACTATTAATCTTTACGCGGTCATGGCGAAATACAATTGTAAAAAGATGGTTTTTTCATCATCTGCAACTGTGTATGGACAACCTGAAAAAATTCCATGTGTTGAGGATTTCAACCTTGTAGCTTTGAATCCATATGGCCGTACCAAGCTTTTCCTTGAAGAAATTGCTCGAGATATGCATAATGCAGACCCAGATTGGCAAATTATTCTGCTGAGGTACTTCAATCCTGTCGGGGCTCATGAGAGTGGGAAAATTGGTGAAGATCCCAAAGGTATCCCAAACAACCTCATGCCGTACATACAGCAAGTAGCTGTTGGAAGATTGCCTGAGCTTAATGTCTTTGGTCATGATTATCCCACCAAGGATGGTACTGCGATCCGAGACTACATCCATGTTATGGACTTAGCAGATGGTCATATTGCTGCCCTTCAGAAGCTTTTTACAACAGAGAATATAGGTTGTGCTGTCTACAATCTCGGAACTGGACAAGGTACATCGGTGCTTGAAATGGTTTCTGGTTTCGAGAAAGCTTCTGGCAAGAAAATTCCTATCAAACTATGTCCGAGGAGGCCAGGAGATGCTACTGCTGTTTATGCTTCAACAGAGAAAGCTGAAAGGGAACTCGGGTGGAAGGCAAAATACGGAATAGAGGAGATGTGCAGGGACCAATGGAAGTGGGCAGTAAACAATCCTCACGGTTACAAGTCGAAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00520 ko01100 map00052 map00520 map01100
Pfam Domains
Protein Families
GDP_Man_Dehyd

Protein Analysis

351

Amino Acids

38.85

Weight (kDa)

6.6

Isoelectric Point (pI)

26.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 6 - 123 8.7e-09 short chain dehydrogenase
RmlD_sub_bind PF04321 7 - 170 2.5e-09 RmlD substrate binding domain
Epimerase PF01370 8 - 263 3.6e-57 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 8 - 190 1.2e-15 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 9 - 334 1.5e-67 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 9 - 139 9.8e-14 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 12 - 181 3.4e-06 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12780 AT1G63180 AT1G63180
fragaria_vesca FvH4_5g01780 FvH4_5g01780
malus_domestica MD04G1064600.v1.1
prunus_persica Prupe.5G093300_v2.0.a1 Prupe.5G093300_v2.0.a1
pyrus_communis pycom04g05840
rosa_chinensis RchiOBHm_Chr7g0198901
rosa_laevigata RLG00000003834
rosa_multiflora Rmu_sc0016854.1_g000005
rosa_roxburghii Rroxscaffold_3G00257320
rosa_rugosa Rorug07G0050500 Rorug07G0050600
rosa_samantha Rh7BG177400 Rh7CG183400 Rh7DG176800
rosa_wichuraiana Rw7G015260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 64, 733
AccI GTMKAC 1 cut(s) 804
AccII CGCG 3 cut(s) 41, 144, 353
AciI CCGC 5 cut(s) 36, 41, 83, 305, 353
AclWI GGATC 2 cut(s) 593, 706
AcoI YGGCCR 1 cut(s) 472
AcsI RAATTY 2 cut(s) 426, 879
AcuI CTGAAG 2 cut(s) 150, 749
AdeI CACNNNGTG 1 cut(s) 64
AfaI GTAC 5 cut(s) 478, 554, 632, 706, 829
AfiI CCNNNNNNNGG 2 cut(s) 64, 733
AgsI TTSAA 6 cut(s) 448, 463, 494, 559, 842, 936
AjnI CCWGG 1 cut(s) 907
Alw26I GTCTC 1 cut(s) 711
AlwI GGATC 2 cut(s) 593, 706
Ama87I CYCGRG 3 cut(s) 77, 504, 959
AoxI GGCC 4 cut(s) 160, 271, 472, 905
ApeKI GCWGC 2 cut(s) 73, 758
ApoI RAATTY 2 cut(s) 426, 879
AseI ATTAAT 1 cut(s) 342
Asp700I GAANNNNTTC 1 cut(s) 426
AspLEI GCGC 1 cut(s) 41
AspS9I GGNCC 2 cut(s) 161, 1000
AsuHPI GGTGA 3 cut(s) 40, 209, 605
AvaI CYCGRG 3 cut(s) 77, 504, 959
AvaII GGWCC 1 cut(s) 1000
BaeI ACNNNNGTAYC 2 cut(s) 819, 852
BanII GRGCYC 1 cut(s) 574
BarI GAAGNNNNNNTAC 2 cut(s) 959, 991
BbvCI CCTCAGC 1 cut(s) 548
BbvI GCAGC 2 cut(s) 60, 745
BccI CCATC 3 cut(s) 376, 695, 740
BceAI ACGGC 2 cut(s) 459, 613
BciT130I CCWGG 1 cut(s) 909
BciVI GTATCC 1 cut(s) 620
BcoDI GTCTC 1 cut(s) 711
BfmI CTRYAG 1 cut(s) 282
BfuI GTATCC 1 cut(s) 620
BisI GCNGC 3 cut(s) 37, 74, 759
BlsI GCNGC 3 cut(s) 38, 75, 760
Bme1390I CCNGG 1 cut(s) 909
Bme18I GGWCC 1 cut(s) 1000
BmeT110I CYCGRG 3 cut(s) 77, 504, 959
BmgT120I GGNCC 2 cut(s) 161, 1000
BmiI GGNNCC 3 cut(s) 56, 163, 1001
BmrFI CCNGG 1 cut(s) 909
BmsI GCATC 2 cut(s) 241, 904
Bpu10I CCTNAGC 2 cut(s) 548, 663
BsaJI CCNNGG 2 cut(s) 696, 899
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 279, 309
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 733
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 823
BseBI CCWGG 1 cut(s) 909
BseDI CCNNGG 2 cut(s) 696, 899
BseGI GGATG 2 cut(s) 706, 723
BseLI CCNNNNNNNGG 2 cut(s) 64, 733
BseMII CTCAG 4 cut(s) 188, 217, 539, 654
BseNI ACTGG 1 cut(s) 823
BseRI GAGGAG 2 cut(s) 916, 1001
BseXI GCAGC 2 cut(s) 60, 745
BseYI CCCAGC 1 cut(s) 193
BsgI GTGCAG 1 cut(s) 1015
Bsh1236I CGCG 3 cut(s) 41, 144, 353
BshFI GGCC 4 cut(s) 162, 273, 474, 907
BsiHKCI CYCGRG 3 cut(s) 77, 504, 959
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 2 cut(s) 164, 1013
BslI CCNNNNNNNGG 2 cut(s) 64, 733
BsmAI GTCTC 1 cut(s) 711
BsmFI GGGAC 2 cut(s) 164, 1013
BsnI GGCC 4 cut(s) 162, 273, 474, 907
BsoBI CYCGRG 3 cut(s) 77, 504, 959
Bsp1286I GDGCHC 1 cut(s) 574
Bsp143I GATC 4 cut(s) 139, 199, 598, 711
BspACI CCGC 5 cut(s) 36, 41, 83, 305, 353
BspANI GGCC 4 cut(s) 162, 273, 474, 907
BspCNI CTCAG 4 cut(s) 187, 216, 540, 655
BspFNI CGCG 3 cut(s) 41, 144, 353
BspHI TCATGA 2 cut(s) 574, 682
BspLI GGNNCC 3 cut(s) 56, 163, 1001
BspPI GGATC 2 cut(s) 593, 706
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 1 cut(s) 823
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 2 cut(s) 696, 899
BssMI GATC 4 cut(s) 139, 199, 598, 711
BssT1I CCWWGG 1 cut(s) 696
Bst2UI CCWGG 1 cut(s) 909
Bst4CI ACNGT 4 cut(s) 64, 311, 406, 1034
BstC8I GCNNGC 1 cut(s) 271
BstDEI CTNAG 5 cut(s) 174, 203, 548, 663, 739
BstEII GGTNACC 1 cut(s) 28
BstF5I GGATG 2 cut(s) 706, 723
BstFNI CGCG 3 cut(s) 41, 144, 353
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 4 cut(s) 142, 202, 601, 714
BstMAI GTCTC 1 cut(s) 711
BstMBI GATC 4 cut(s) 139, 199, 598, 711
BstMWI GCNNNNNNNGC 2 cut(s) 359, 929
BstNI CCWGG 1 cut(s) 909
BstPI GGTNACC 1 cut(s) 28
BstSCI CCNGG 1 cut(s) 907
BstSFI CTRYAG 1 cut(s) 282
BstUI CGCG 3 cut(s) 41, 144, 353
BstV1I GCAGC 2 cut(s) 60, 745
BstX2I RGATCY 1 cut(s) 598
BstXI CCANNNNNNTGG 1 cut(s) 269
BstYI RGATCY 1 cut(s) 598
BsuI GTATCC 1 cut(s) 620
BsuRI GGCC 4 cut(s) 162, 273, 474, 907
BtsCI GGATG 2 cut(s) 706, 723
Cac8I GCNNGC 1 cut(s) 271
CciI TCATGA 2 cut(s) 574, 682
CfoI GCGC 1 cut(s) 41
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 161, 1000
CseI GACGC 1 cut(s) 133
Csp6I GTAC 5 cut(s) 477, 553, 631, 705, 828
CspCI CAANNNNNGTGG 2 cut(s) 992, 1027
CviAII CATG 6 cut(s) 358, 432, 575, 625, 683, 728
CviQI GTAC 5 cut(s) 477, 553, 631, 705, 828
DdeI CTNAG 5 cut(s) 174, 203, 548, 663, 739
DpnI GATC 4 cut(s) 141, 201, 600, 713
DpnII GATC 4 cut(s) 139, 199, 598, 711
DraIII CACNNNGTG 1 cut(s) 64
EaeI YGGCCR 1 cut(s) 472
Eco130I CCWWGG 1 cut(s) 696
Eco24I GRGCYC 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 1000
Eco57I CTGAAG 2 cut(s) 150, 749
Eco88I CYCGRG 3 cut(s) 77, 504, 959
Eco91I GGTNACC 1 cut(s) 28
EcoO65I GGTNACC 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 907
EcoT14I CCWWGG 1 cut(s) 696
EcoT22I ATGCAT 1 cut(s) 516
EcoT38I GRGCYC 1 cut(s) 574
ErhI CCWWGG 1 cut(s) 696
FaeI CATG 6 cut(s) 361, 435, 578, 628, 686, 731
FaqI GGGAC 2 cut(s) 164, 1013
FatI CATG 6 cut(s) 357, 431, 574, 624, 682, 727
FauI CCCGC 3 cut(s) 34, 76, 312
FauNDI CATATG 1 cut(s) 469
FblI GTMKAC 1 cut(s) 804
Fnu4HI GCNGC 3 cut(s) 37, 74, 759
FokI GGATG 2 cut(s) 710, 713
FriOI GRGCYC 1 cut(s) 574
Fsp4HI GCNGC 3 cut(s) 37, 74, 759
GlaI GCGC 1 cut(s) 40
GluI GCNGC 3 cut(s) 37, 74, 759
GsaI CCCAGC 1 cut(s) 197
HaeIII GGCC 4 cut(s) 162, 273, 474, 907
HapII CCGG 1 cut(s) 33
HgaI GACGC 1 cut(s) 133
HhaI GCGC 1 cut(s) 41
Hin1II CATG 6 cut(s) 361, 435, 578, 628, 686, 731
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HindIII AAGCTT 3 cut(s) 482, 770, 864
HinfI GANTC 2 cut(s) 22, 463
HpaII CCGG 1 cut(s) 33
HphI GGTGA 3 cut(s) 40, 209, 605
Hpy166II GTNNAC 2 cut(s) 805, 1021
Hpy188I TCNGA 6 cut(s) 177, 206, 716, 768, 815, 900
Hpy188III TCNNGA 5 cut(s) 113, 506, 575, 683, 859
Hpy8I GTNNAC 2 cut(s) 805, 1021
HpyAV CCTTC 2 cut(s) 773, 961
HpyCH4III ACNGT 4 cut(s) 64, 311, 406, 1034
HpyCH4V TGCA 5 cut(s) 299, 401, 514, 521, 996
HpyF10VI GCNNNNNNNGC 2 cut(s) 359, 929
HpyF3I CTNAG 5 cut(s) 174, 203, 548, 663, 739
Hsp92II CATG 6 cut(s) 361, 435, 578, 628, 686, 731
HspAI GCGC 1 cut(s) 39
Kzo9I GATC 4 cut(s) 139, 199, 598, 711
LmnI GCTCC 2 cut(s) 13, 54
Lsp1109I GCAGC 2 cut(s) 60, 745
LweI GCATC 2 cut(s) 241, 904
MaeIII GTNAC 2 cut(s) 28, 1034
MalI GATC 4 cut(s) 141, 201, 600, 713
MboI GATC 4 cut(s) 139, 199, 598, 711
MboII GAAGA 3 cut(s) 506, 608, 666
MfeI CAATTG 1 cut(s) 370
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 1 cut(s) 574
MluCI AATT 7 cut(s) 325, 370, 426, 498, 537, 588, 879
MmeI TCCRAC 2 cut(s) 313, 631
MnlI CCTC 8 cut(s) 73, 433, 543, 632, 894, 897, 979, 1038
Mph1103I ATGCAT 1 cut(s) 516
MroXI GAANNNNTTC 1 cut(s) 426
MseI TTAA 2 cut(s) 342, 669
MslI CAYNNNNRTG 1 cut(s) 579
MspA1I CMGCKG 2 cut(s) 73, 193
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 909
MunI CAATTG 1 cut(s) 370
MvaI CCWGG 1 cut(s) 909
MvnI CGCG 3 cut(s) 41, 144, 353
MwoI GCNNNNNNNGC 2 cut(s) 359, 929
NdeI CATATG 1 cut(s) 469
NdeII GATC 4 cut(s) 139, 199, 598, 711
NlaIII CATG 6 cut(s) 361, 435, 578, 628, 686, 731
NlaIV GGNNCC 3 cut(s) 56, 163, 1001
NmuCI GTSAC 1 cut(s) 28
NsiI ATGCAT 1 cut(s) 516
PaeR7I CTCGAG 2 cut(s) 77, 504
PagI TCATGA 2 cut(s) 574, 682
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PdmI GAANNNNTTC 1 cut(s) 426
PfeI GAWTC 2 cut(s) 22, 463
PflFI GACNNNGTC 1 cut(s) 155
PflMI CCANNNNNTGG 2 cut(s) 64, 733
PkrI GCNGC 3 cut(s) 38, 75, 760
PshBI ATTAAT 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 907
PspEI GGTNACC 1 cut(s) 28
PspFI CCCAGC 1 cut(s) 193
PspGI CCWGG 1 cut(s) 907
PspN4I GGNNCC 3 cut(s) 56, 163, 1001
PspPI GGNCC 2 cut(s) 161, 1000
PspXI VCTCGAGB 1 cut(s) 77
PsuI RGATCY 1 cut(s) 598
PsyI GACNNNGTC 1 cut(s) 155
PvuII CAGCTG 2 cut(s) 73, 193
RsaI GTAC 5 cut(s) 478, 554, 632, 706, 829
RsaNI GTAC 5 cut(s) 477, 553, 631, 705, 828
RseI CAYNNNNRTG 1 cut(s) 579
SaqAI TTAA 2 cut(s) 342, 669
SatI GCNGC 3 cut(s) 37, 74, 759
Sau3AI GATC 4 cut(s) 139, 199, 598, 711
Sau96I GGNCC 2 cut(s) 161, 1000
ScrFI CCNGG 1 cut(s) 909
SduI GDGCHC 1 cut(s) 574
SfaNI GCATC 2 cut(s) 241, 904
SfcI CTRYAG 1 cut(s) 282
Sfr274I CTCGAG 2 cut(s) 77, 504
SinI GGWCC 1 cut(s) 1000
SlaI CTCGAG 2 cut(s) 77, 504
SmiMI CAYNNNNRTG 1 cut(s) 579
SmlI CTYRAG 2 cut(s) 77, 504
SmoI CTYRAG 2 cut(s) 77, 504
Sse9I AATT 7 cut(s) 325, 370, 426, 498, 537, 588, 879
SsiI CCGC 5 cut(s) 36, 41, 83, 305, 353
StyD4I CCNGG 1 cut(s) 907
StyI CCWWGG 1 cut(s) 696
TaaI ACNGT 4 cut(s) 64, 311, 406, 1034
TaqI TCGA 5 cut(s) 78, 114, 505, 858, 1043
TasI AATT 7 cut(s) 325, 370, 426, 498, 537, 588, 879
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 2 cut(s) 22, 463
Tru1I TTAA 2 cut(s) 342, 669
Tru9I TTAA 2 cut(s) 342, 669
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 2 cut(s) 73, 758
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 2 cut(s) 37, 381
TspGWI ACGGA 3 cut(s) 88, 112, 993
Tth111I GACNNNGTC 1 cut(s) 155
Van91I CCANNNNNTGG 2 cut(s) 64, 733
VpaK11BI GGWCC 1 cut(s) 1000
VspI ATTAAT 1 cut(s) 342
XapI RAATTY 2 cut(s) 426, 879
XhoI CTCGAG 2 cut(s) 77, 504
XmiI GTMKAC 1 cut(s) 804
XmnI GAANNNNTTC 1 cut(s) 426
Zsp2I ATGCAT 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.