pycom04g06900

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013944
Physical Location & Seq
Forward (+)
7185242 .. 7189438
4197 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 960 bp
ATGGAGGGTAGGGAAGGTGGTGTGAATAGTAGTGGGGTTACAGTTGTGGGATCAGATGCTCCATCAGACTACCATGTCGCTCCAAGGTCAGAAAACCCGACTCACAACGATCGATCAACACCTCAGGCGCCGGTTGCGCCGCCACAGGCGGCGTCTGTGCCGGCGGCAGGGTTTATGCCGATGAAGAAGAAGAGAGGGAGGCCAAGGAAGTACGGACCGGACGGGAGTGTCACCATGGCTCTGTCACCGAAGCCCATATCGTCCTCTGCGCCGCCGCCGGTGATCGATTTCTCGGCGGAGAAGCGTGGGAAAGTGAAGCCACCCAGCTCAGTGAGCAAGACCAAGTACGAGGTGGAGAATTTAGGTGAATGGGTTGCATGCTCGGTTGGTGCTAATTTTACACCCCATATTATCACTGTTAATTCGGGAGAGGATGTCATGATGAAGATTATATCATTTTCTCAACAAGGTCCTCGAGCAATATGCGTGCTCTCTGCCAATGGTGTTATTTCAAGTGTGACTCTTCGTCAGCCTGATTCTTCTGGCGGTACATTGACATACGAGGGCCGTTTCGAGATATTGTCTTTATCGGGTTCATTTATGCCCAATGAAACAGGAGGGACAAGAAGCAGATCAGGCGGGATGAGCGTTTCTTTAGCAAGTCCAGATGGGCGTGTTGTAGGTGGTGGAGTTGGTGGTCTGTTAGTAGCTGCAAGTCCAGTGCAGGTTGTAGTAGGCAGTTTTCTATCAGGAAACCAGCATGAACAGAAGCCTAAGAAACAGAAACATGATTATATAGCTAATGCAACACCAACTATGGCTGTTCCTATTTCAAGTGCCGACCCAAAACCAAACTTCTCATCCTCGACTTCCTTCCGTGGAGATAATTGGTCGTCATTGGCATCAGATCCAAAAAACAAGACTGACATTAATGTATCTTTGCCGGGAGGCGTAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

320

Amino Acids

33.13

Weight (kDa)

9.39

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 134 - 248 2.6e-26 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12080
fragaria_vesca FvH4_5g00860
malus_domestica MD00G1083100.v1.1 MD06G1063800.v1.1
prunus_persica Prupe.5G081500_v2.0.a1
pyrus_communis pycom04g06900 pycom06g06420
rosa_chinensis RchiOBHm_Chr7g0201041
rosa_laevigata RLG00000003696
rosa_multiflora Rmu_sc0006553.1_g000014
rosa_roxburghii Rroxscaffold_3G00255570
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187500 Rh7BG190800 Rh7CG198500 Rh7DG193100
rosa_wichuraiana Rw7G016450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 74, 227
Acc36I ACCTGC 1 cut(s) 715
AccB1I GGYRCC 1 cut(s) 127
AciI CCGC 8 cut(s) 140, 149, 164, 272, 275, 296, 546, 639
AclWI GGATC 2 cut(s) 58, 902
AcsI RAATTY 1 cut(s) 358
AcyI GRCGYC 2 cut(s) 128, 152
AfaI GTAC 3 cut(s) 212, 347, 550
AfiI CCNNNNNNNGG 1 cut(s) 167
AgsI TTSAA 2 cut(s) 513, 834
AhdI GACNNNNNGTC 1 cut(s) 525
AluBI AGCT 3 cut(s) 327, 710, 800
AluI AGCT 3 cut(s) 327, 710, 800
Alw21I GWGCWC 1 cut(s) 492
AlwI GGATC 2 cut(s) 58, 902
Ama87I CYCGRG 1 cut(s) 474
AoxI GGCC 2 cut(s) 200, 565
ApeKI GCWGC 1 cut(s) 710
ApoI RAATTY 1 cut(s) 358
AseI ATTAAT 1 cut(s) 930
AspLEI GCGC 3 cut(s) 130, 139, 271
AspS9I GGNCC 3 cut(s) 215, 470, 565
AsuC2I CCSGG 1 cut(s) 945
AsuHPI GGTGA 4 cut(s) 223, 237, 292, 377
AvaI CYCGRG 1 cut(s) 474
AvaII GGWCC 2 cut(s) 215, 470
AxyI CCTNAGG 1 cut(s) 123
BanI GGYRCC 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 492
BbvI GCAGC 1 cut(s) 697
BccI CCATC 2 cut(s) 70, 662
BceAI ACGGC 1 cut(s) 552
BcgI CGANNNNNNTGC 2 cut(s) 465, 499
BcnI CCSGG 1 cut(s) 945
BfoI RGCGCY 1 cut(s) 131
BfuAI ACCTGC 1 cut(s) 715
BisI GCNGC 6 cut(s) 140, 150, 165, 272, 275, 711
BlsI GCNGC 6 cut(s) 141, 151, 166, 273, 276, 712
Bme1390I CCNGG 1 cut(s) 945
Bme18I GGWCC 2 cut(s) 215, 470
BmeRI GACNNNNNGTC 1 cut(s) 525
BmeT110I CYCGRG 1 cut(s) 474
BmgT120I GGNCC 3 cut(s) 215, 470, 565
BmiI GGNNCC 1 cut(s) 129
BmrFI CCNGG 1 cut(s) 945
BmsI GCATC 2 cut(s) 46, 911
BpuMI CCSGG 1 cut(s) 945
Bsa29I ATCGAT 2 cut(s) 112, 285
BsaHI GRCGYC 2 cut(s) 128, 152
BsaJI CCNNGG 4 cut(s) 83, 203, 234, 877
BsaWI WCCGGW 1 cut(s) 217
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 167
Bse118I RCCGGY 3 cut(s) 130, 160, 277
Bse1I ACTGG 1 cut(s) 719
Bse21I CCTNAGG 1 cut(s) 123
BseCI ATCGAT 2 cut(s) 112, 285
BseDI CCNNGG 4 cut(s) 83, 203, 234, 877
BseGI GGATG 3 cut(s) 439, 648, 860
BseLI CCNNNNNNNGG 1 cut(s) 167
BseMII CTCAG 2 cut(s) 137, 342
BseNI ACTGG 1 cut(s) 719
BseXI GCAGC 1 cut(s) 697
BseYI CCCAGC 1 cut(s) 323
BsgI GTGCAG 1 cut(s) 743
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 2 cut(s) 202, 567
BshNI GGYRCC 1 cut(s) 127
BshVI ATCGAT 2 cut(s) 112, 285
BsiEI CGRYCG 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 492
BsiHKCI CYCGRG 1 cut(s) 474
BsiSI CCGG 5 cut(s) 131, 161, 218, 278, 944
BslFI GGGAC 1 cut(s) 634
BslI CCNNNNNNNGG 1 cut(s) 167
BsmFI GGGAC 1 cut(s) 634
BsnI GGCC 2 cut(s) 202, 567
BsoBI CYCGRG 1 cut(s) 474
Bsp1286I GDGCHC 1 cut(s) 492
Bsp143I GATC 6 cut(s) 50, 109, 113, 282, 632, 907
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 8 cut(s) 140, 149, 164, 272, 275, 296, 546, 639
BspANI GGCC 2 cut(s) 202, 567
BspCNI CTCAG 2 cut(s) 136, 341
BspDI ATCGAT 2 cut(s) 112, 285
BspHI TCATGA 1 cut(s) 438
BspLI GGNNCC 1 cut(s) 129
BspMI ACCTGC 1 cut(s) 715
BspPI GGATC 2 cut(s) 58, 902
BspT107I GGYRCC 1 cut(s) 127
BsrFI RCCGGY 3 cut(s) 130, 160, 277
BsrI ACTGG 1 cut(s) 719
BssAI RCCGGY 3 cut(s) 130, 160, 277
BssECI CCNNGG 4 cut(s) 83, 203, 234, 877
BssMI GATC 6 cut(s) 50, 109, 113, 282, 632, 907
BssNI GRCGYC 2 cut(s) 128, 152
BssT1I CCWWGG 3 cut(s) 83, 203, 234
Bst4CI ACNGT 2 cut(s) 43, 418
Bst6I CTCTTC 2 cut(s) 185, 528
BstACI GRCGYC 2 cut(s) 128, 152
BstC8I GCNNGC 3 cut(s) 162, 379, 488
BstDEI CTNAG 3 cut(s) 123, 328, 774
BstDSI CCRYGG 2 cut(s) 234, 877
BstF5I GGATG 3 cut(s) 439, 648, 860
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 3 cut(s) 130, 139, 271
BstKTI GATC 6 cut(s) 53, 112, 116, 285, 635, 910
BstMBI GATC 6 cut(s) 50, 109, 113, 282, 632, 907
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 5 cut(s) 134, 136, 333, 636, 645
BstNSI RCATGY 1 cut(s) 381
BstSCI CCNGG 1 cut(s) 943
BstV1I GCAGC 1 cut(s) 697
BstX2I RGATCY 1 cut(s) 907
BstYI RGATCY 1 cut(s) 907
Bsu15I ATCGAT 2 cut(s) 112, 285
Bsu36I CCTNAGG 1 cut(s) 123
BsuRI GGCC 2 cut(s) 202, 567
BsuTUI ATCGAT 2 cut(s) 112, 285
BtgI CCRYGG 2 cut(s) 234, 877
BtsCI GGATG 3 cut(s) 439, 648, 860
BtsIMutI CAGTG 3 cut(s) 336, 414, 726
BveI ACCTGC 1 cut(s) 715
Cac8I GCNNGC 3 cut(s) 162, 379, 488
CciI TCATGA 1 cut(s) 438
CfoI GCGC 3 cut(s) 130, 139, 271
Cfr10I RCCGGY 3 cut(s) 130, 160, 277
Cfr13I GGNCC 3 cut(s) 215, 470, 565
ClaI ATCGAT 2 cut(s) 112, 285
CpoI CGGWCCG 1 cut(s) 215
CseI GACGC 1 cut(s) 141
Csp6I GTAC 3 cut(s) 211, 346, 549
CspI CGGWCCG 1 cut(s) 215
CviAII CATG 6 cut(s) 74, 235, 378, 439, 761, 788
CviQI GTAC 3 cut(s) 211, 346, 549
DdeI CTNAG 3 cut(s) 123, 328, 774
DinI GGCGCC 1 cut(s) 129
DpnI GATC 6 cut(s) 52, 111, 115, 284, 634, 909
DpnII GATC 6 cut(s) 50, 109, 113, 282, 632, 907
DrdI GACNNNNNNGTC 2 cut(s) 74, 227
DriI GACNNNNNGTC 1 cut(s) 525
DseDI GACNNNNNNGTC 2 cut(s) 74, 227
Eam1104I CTCTTC 2 cut(s) 185, 528
Eam1105I GACNNNNNGTC 1 cut(s) 525
EarI CTCTTC 2 cut(s) 185, 528
EciI GGCGGA 1 cut(s) 311
Eco130I CCWWGG 3 cut(s) 83, 203, 234
Eco47I GGWCC 2 cut(s) 215, 470
Eco81I CCTNAGG 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 474
EcoO109I RGGNCCY 1 cut(s) 470
EcoT14I CCWWGG 3 cut(s) 83, 203, 234
EgeI GGCGCC 1 cut(s) 129
EheI GGCGCC 1 cut(s) 129
ErhI CCWWGG 3 cut(s) 83, 203, 234
FaeI CATG 6 cut(s) 77, 238, 381, 442, 764, 791
FaqI GGGAC 1 cut(s) 634
FatI CATG 6 cut(s) 73, 234, 377, 438, 760, 787
FauI CCCGC 1 cut(s) 632
Fnu4HI GCNGC 6 cut(s) 140, 150, 165, 272, 275, 711
FokI GGATG 3 cut(s) 446, 655, 847
Fsp4HI GCNGC 6 cut(s) 140, 150, 165, 272, 275, 711
GlaI GCGC 3 cut(s) 129, 138, 270
GluI GCNGC 6 cut(s) 140, 150, 165, 272, 275, 711
GsaI CCCAGC 1 cut(s) 327
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 2 cut(s) 202, 567
HapII CCGG 5 cut(s) 131, 161, 218, 278, 944
HgaI GACGC 1 cut(s) 141
HhaI GCGC 3 cut(s) 130, 139, 271
Hin1I GRCGYC 2 cut(s) 128, 152
Hin1II CATG 6 cut(s) 77, 238, 381, 442, 764, 791
Hin6I GCGC 3 cut(s) 128, 137, 269
HinP1I GCGC 3 cut(s) 128, 137, 269
HinfI GANTC 3 cut(s) 100, 520, 536
HpaII CCGG 5 cut(s) 131, 161, 218, 278, 944
HphI GGTGA 4 cut(s) 223, 237, 292, 377
Hpy188I TCNGA 4 cut(s) 55, 67, 91, 907
Hpy188III TCNNGA 5 cut(s) 426, 439, 574, 665, 750
HpyAV CCTTC 2 cut(s) 8, 883
HpyCH4III ACNGT 2 cut(s) 43, 418
HpyCH4V TGCA 4 cut(s) 377, 713, 724, 806
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 136, 333, 636, 645
HpyF3I CTNAG 3 cut(s) 123, 328, 774
Hsp92I GRCGYC 2 cut(s) 128, 152
Hsp92II CATG 6 cut(s) 77, 238, 381, 442, 764, 791
HspAI GCGC 3 cut(s) 128, 137, 269
KasI GGCGCC 1 cut(s) 127
KroI GCCGGC 1 cut(s) 160
KroNI GCCGGC 1 cut(s) 162
Kzo9I GATC 6 cut(s) 50, 109, 113, 282, 632, 907
LmnI GCTCC 2 cut(s) 64, 85
Lsp1109I GCAGC 1 cut(s) 697
LweI GCATC 2 cut(s) 46, 911
MaeIII GTNAC 4 cut(s) 37, 229, 243, 517
MalI GATC 6 cut(s) 52, 111, 115, 284, 634, 909
MboI GATC 6 cut(s) 50, 109, 113, 282, 632, 907
MboII GAAGA 6 cut(s) 196, 199, 202, 457, 515, 531
MflI RGATCY 1 cut(s) 907
MhlI GDGCHC 1 cut(s) 492
MluCI AATT 4 cut(s) 358, 394, 421, 886
Mly113I GGCGCC 1 cut(s) 128
MlyI GAGTC 2 cut(s) 94, 514
MroNI GCCGGC 1 cut(s) 160
MseI TTAA 2 cut(s) 420, 930
MspI CCGG 5 cut(s) 131, 161, 218, 278, 944
MspR9I CCNGG 1 cut(s) 945
MwoI GCNNNNNNNGC 5 cut(s) 134, 136, 333, 636, 645
NaeI GCCGGC 1 cut(s) 162
NarI GGCGCC 1 cut(s) 128
NciI CCSGG 1 cut(s) 945
NcoI CCATGG 1 cut(s) 234
NdeII GATC 6 cut(s) 50, 109, 113, 282, 632, 907
NgoMIV GCCGGC 1 cut(s) 160
NlaIII CATG 6 cut(s) 77, 238, 381, 442, 764, 791
NlaIV GGNNCC 1 cut(s) 129
NmeAIII GCCGAG 1 cut(s) 272
NmuCI GTSAC 3 cut(s) 229, 243, 517
NspI RCATGY 1 cut(s) 381
PaeI GCATGC 1 cut(s) 381
PaeR7I CTCGAG 1 cut(s) 474
PagI TCATGA 1 cut(s) 438
PdiI GCCGGC 1 cut(s) 162
PfeI GAWTC 1 cut(s) 536
PkrI GCNGC 6 cut(s) 141, 151, 166, 273, 276, 712
Ple19I CGATCG 1 cut(s) 112
PleI GAGTC 2 cut(s) 94, 514
PluTI GGCGCC 1 cut(s) 131
PpsI GAGTC 2 cut(s) 94, 514
PpuMI RGGWCCY 1 cut(s) 470
PshBI ATTAAT 1 cut(s) 930
Psp5II RGGWCCY 1 cut(s) 470
PspFI CCCAGC 1 cut(s) 323
PspN4I GGNNCC 1 cut(s) 129
PspPI GGNCC 3 cut(s) 215, 470, 565
PspPPI RGGWCCY 1 cut(s) 470
PspXI VCTCGAGB 1 cut(s) 474
PsuI RGATCY 1 cut(s) 907
PvuI CGATCG 1 cut(s) 112
RsaI GTAC 3 cut(s) 212, 347, 550
RsaNI GTAC 3 cut(s) 211, 346, 549
Rsr2I CGGWCCG 1 cut(s) 215
RsrII CGGWCCG 1 cut(s) 215
SaqAI TTAA 2 cut(s) 420, 930
SatI GCNGC 6 cut(s) 140, 150, 165, 272, 275, 711
Sau3AI GATC 6 cut(s) 50, 109, 113, 282, 632, 907
Sau96I GGNCC 3 cut(s) 215, 470, 565
SchI GAGTC 2 cut(s) 94, 514
ScrFI CCNGG 1 cut(s) 945
SduI GDGCHC 1 cut(s) 492
SfaNI GCATC 2 cut(s) 46, 911
SfoI GGCGCC 1 cut(s) 129
Sfr274I CTCGAG 1 cut(s) 474
SgrAI CRCCGGYG 1 cut(s) 277
SinI GGWCC 2 cut(s) 215, 470
SlaI CTCGAG 1 cut(s) 474
SmlI CTYRAG 1 cut(s) 474
SmoI CTYRAG 1 cut(s) 474
SphI GCATGC 1 cut(s) 381
Sse9I AATT 4 cut(s) 358, 394, 421, 886
SsiI CCGC 8 cut(s) 140, 149, 164, 272, 275, 296, 546, 639
SspDI GGCGCC 1 cut(s) 127
StyD4I CCNGG 1 cut(s) 943
StyI CCWWGG 3 cut(s) 83, 203, 234
TaaI ACNGT 2 cut(s) 43, 418
TaqI TCGA 5 cut(s) 112, 285, 475, 573, 866
TasI AATT 4 cut(s) 358, 394, 421, 886
TauI GCSGC 5 cut(s) 142, 152, 167, 274, 277
TfiI GAWTC 1 cut(s) 536
Tru1I TTAA 2 cut(s) 420, 930
Tru9I TTAA 2 cut(s) 420, 930
TscAI CASTG 3 cut(s) 336, 421, 726
TseFI GTSAC 3 cut(s) 229, 243, 517
TseI GCWGC 1 cut(s) 710
Tsp45I GTSAC 3 cut(s) 229, 243, 517
TspDTI ATGAA 5 cut(s) 197, 458, 585, 624, 777
TspGWI ACGGA 2 cut(s) 228, 866
TspRI CASTG 3 cut(s) 336, 421, 726
VpaK11BI GGWCC 2 cut(s) 215, 470
VspI ATTAAT 1 cut(s) 930
XapI RAATTY 1 cut(s) 358
XceI RCATGY 1 cut(s) 381
XcmI CCANNNNNNNNNTGG 1 cut(s) 349
XhoI CTCGAG 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.