pycom04g06980

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0016839
Physical Location & Seq
Reverse (-)
7250083 .. 7250682
600 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 486 bp
ATGATCTCCAAGATGGCGCTTCATACGTTTCCGCCCGAGAGGCACAGCGGCAAACTCGTCCGGCGTCGAGGAGGTCTCATCTCTGTCCCAGTGAAGCTTTGGCCGGCGCCGGAGACCTACCGAAGGATGACGAAGTTGCTTCTGAATGTGACGATCGAGAGGAGCTTAGGGCCCGTGCAGGTGGTGATGTCGCCGGAAAACACAGTGCGGGATTTGATCAAGGCAGCTGTAGAGATTTACGTGAGGGAGAAGAGAAGGCCGTTGGTAAAGGAGACTGATCCGCACCGTTTTGAGCTCCACTACTCGCAATTCAGCTTGGAGAGTTTGAAAGGAGATGAGAAGTTGATAAAGTTGGTGTCTAGGAATTTCTTTTTGTGCTGCTCAAAGTCAAAGCCCCCCGTCGGTCTGTGTAATTGCTCTGAGGAAGCAAAGAGGGCAATCAGTTATCAGTTTTCATGGATAAAGCTCATGGATTTCTTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

18.64

Weight (kDa)

9.84

Isoelectric Point (pI)

58.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 43 - 126 1.1e-37 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016839)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00830
malus_domestica MD04G1077900.v1.1
prunus_persica Prupe.5G080800_v2.0.a1
pyrus_communis pycom04g06980
rosa_chinensis RchiOBHm_Chr7g0201091
rosa_laevigata RLG00000003692
rosa_multiflora Rmu_sc0000785.1_g000016
rosa_roxburghii Rroxscaffold_3G00255520
rosa_samantha Rh7AG187900 Rh7BG191400 Rh7CG198800 Rh7DG193600
rosa_wichuraiana Rw7G016500 Rw7G016570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 169
AccB1I GGYRCC 1 cut(s) 106
AciI CCGC 4 cut(s) 32, 48, 208, 281
AclWI GGATC 1 cut(s) 272
AcoI YGGCCR 1 cut(s) 101
AcsI RAATTY 1 cut(s) 364
AcyI GRCGYC 2 cut(s) 64, 107
AfiI CCNNNNNNNGG 2 cut(s) 123, 401
AgsI TTSAA 1 cut(s) 328
AluBI AGCT 6 cut(s) 97, 165, 227, 295, 315, 466
AluI AGCT 6 cut(s) 97, 165, 227, 295, 315, 466
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 3 cut(s) 80, 107, 266
AlwI GGATC 1 cut(s) 272
Ama87I CYCGRG 1 cut(s) 35
AoxI GGCC 3 cut(s) 101, 170, 257
ApaI GGGCCC 1 cut(s) 174
ApeKI GCWGC 2 cut(s) 224, 378
ApoI RAATTY 1 cut(s) 364
AspLEI GCGC 2 cut(s) 19, 109
AspS9I GGNCC 2 cut(s) 170, 171
AsuHPI GGTGA 1 cut(s) 196
AvaI CYCGRG 1 cut(s) 35
BaeGI GKGCMC 1 cut(s) 174
BanI GGYRCC 1 cut(s) 106
BanII GRGCYC 2 cut(s) 174, 297
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 236, 365
BccI CCATC 1 cut(s) 7
BceAI ACGGC 1 cut(s) 244
BclI TGATCA 1 cut(s) 216
BcoDI GTCTC 3 cut(s) 80, 107, 266
BfaI CTAG 1 cut(s) 360
BfmI CTRYAG 1 cut(s) 228
BfoI RGCGCY 2 cut(s) 20, 110
BfuAI ACCTGC 1 cut(s) 169
BglI GCCNNNNNGGC 1 cut(s) 40
BisI GCNGC 3 cut(s) 49, 225, 379
BlsI GCNGC 3 cut(s) 50, 226, 380
BmeT110I CYCGRG 1 cut(s) 35
BmgT120I GGNCC 2 cut(s) 170, 171
BmiI GGNNCC 2 cut(s) 108, 172
BmrI ACTGGG 1 cut(s) 83
BmuI ACTGGG 1 cut(s) 83
BplI GAGNNNNNCTC 2 cut(s) 60, 92
Bpu10I CCTNAGC 1 cut(s) 166
BsaAI YACGTR 1 cut(s) 241
BsaHI GRCGYC 2 cut(s) 64, 107
BsaI GGTCTC 2 cut(s) 80, 107
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 401
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 1 cut(s) 89
BseGI GGATG 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 123, 401
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 2 cut(s) 84, 175
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 2 cut(s) 236, 365
BsgI GTGCAG 1 cut(s) 197
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 3 cut(s) 103, 172, 259
BshNI GGYRCC 1 cut(s) 106
BsiEI CGRYCG 1 cut(s) 156
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 35
BsiSI CCGG 4 cut(s) 61, 104, 110, 194
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 2 cut(s) 123, 401
BsmAI GTCTC 3 cut(s) 80, 107, 266
BsmFI GGGAC 1 cut(s) 71
BsnI GGCC 3 cut(s) 103, 172, 259
Bso31I GGTCTC 2 cut(s) 80, 107
BsoBI CYCGRG 1 cut(s) 35
Bsp120I GGGCCC 1 cut(s) 170
Bsp1286I GDGCHC 2 cut(s) 174, 297
Bsp143I GATC 4 cut(s) 3, 153, 216, 277
BspACI CCGC 4 cut(s) 32, 48, 208, 281
BspANI GGCC 3 cut(s) 103, 172, 259
BspCNI CTCAG 1 cut(s) 412
BspLI GGNNCC 2 cut(s) 108, 172
BspMI ACCTGC 1 cut(s) 169
BspPI GGATC 1 cut(s) 272
BspT107I GGYRCC 1 cut(s) 106
BspTNI GGTCTC 2 cut(s) 80, 107
BsrFI RCCGGY 1 cut(s) 103
BsrI ACTGG 1 cut(s) 89
BssAI RCCGGY 1 cut(s) 103
BssMI GATC 4 cut(s) 3, 153, 216, 277
BssNI GRCGYC 2 cut(s) 64, 107
Bst4CI ACNGT 2 cut(s) 205, 287
Bst6I CTCTTC 1 cut(s) 245
BstACI GRCGYC 2 cut(s) 64, 107
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 1 cut(s) 105
BstDEI CTNAG 2 cut(s) 166, 420
BstENI CCTNNNNNAGG 1 cut(s) 121
BstF5I GGATG 1 cut(s) 132
BstH2I RGCGCY 2 cut(s) 20, 110
BstHHI GCGC 2 cut(s) 19, 109
BstKTI GATC 4 cut(s) 6, 156, 219, 280
BstMAI GTCTC 3 cut(s) 80, 107, 266
BstMBI GATC 4 cut(s) 3, 153, 216, 277
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 2 cut(s) 40, 434
BstSFI CTRYAG 1 cut(s) 228
BstSLI GKGCMC 1 cut(s) 174
BstV1I GCAGC 2 cut(s) 236, 365
BsuRI GGCC 3 cut(s) 103, 172, 259
BtsCI GGATG 1 cut(s) 132
BtsIMutI CAGTG 2 cut(s) 96, 210
BveI ACCTGC 1 cut(s) 169
Cac8I GCNNGC 1 cut(s) 105
CfoI GCGC 2 cut(s) 19, 109
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 170, 171
CseI GACGC 1 cut(s) 53
CviAII CATG 2 cut(s) 456, 469
DdeI CTNAG 2 cut(s) 166, 420
DinI GGCGCC 1 cut(s) 108
DpnI GATC 4 cut(s) 5, 155, 218, 279
DpnII GATC 4 cut(s) 3, 153, 216, 277
EaeI YGGCCR 1 cut(s) 101
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
EciI GGCGGA 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 295
Eco24I GRGCYC 2 cut(s) 174, 297
Eco31I GGTCTC 2 cut(s) 80, 107
Eco53kI GAGCTC 1 cut(s) 295
Eco88I CYCGRG 1 cut(s) 35
EcoICRI GAGCTC 1 cut(s) 295
EcoNI CCTNNNNNAGG 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 170
EcoT38I GRGCYC 2 cut(s) 174, 297
EgeI GGCGCC 1 cut(s) 108
EheI GGCGCC 1 cut(s) 108
FaeI CATG 2 cut(s) 459, 472
FaiI YATR 3 cut(s) 24, 457, 470
FaqI GGGAC 1 cut(s) 71
FatI CATG 2 cut(s) 455, 468
FauI CCCGC 1 cut(s) 201
FbaI TGATCA 1 cut(s) 216
Fnu4HI GCNGC 3 cut(s) 49, 225, 379
FokI GGATG 1 cut(s) 139
FriOI GRGCYC 2 cut(s) 174, 297
Fsp4HI GCNGC 3 cut(s) 49, 225, 379
FspBI CTAG 1 cut(s) 360
GlaI GCGC 2 cut(s) 18, 108
GluI GCNGC 3 cut(s) 49, 225, 379
HaeII RGCGCY 2 cut(s) 20, 110
HaeIII GGCC 3 cut(s) 103, 172, 259
HapII CCGG 4 cut(s) 61, 104, 110, 194
HgaI GACGC 1 cut(s) 53
HhaI GCGC 2 cut(s) 19, 109
Hin1I GRCGYC 2 cut(s) 64, 107
Hin1II CATG 2 cut(s) 459, 472
Hin6I GCGC 2 cut(s) 17, 107
HinP1I GCGC 2 cut(s) 17, 107
HindIII AAGCTT 1 cut(s) 95
HpaII CCGG 4 cut(s) 61, 104, 110, 194
HphI GGTGA 1 cut(s) 196
Hpy188I TCNGA 2 cut(s) 144, 421
Hpy188III TCNNGA 1 cut(s) 157
Hpy99I CGWCG 2 cut(s) 69, 404
HpyAV CCTTC 2 cut(s) 117, 249
HpyCH4III ACNGT 2 cut(s) 205, 287
HpyCH4IV ACGT 2 cut(s) 26, 240
HpyCH4V TGCA 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 434
HpyF3I CTNAG 2 cut(s) 166, 420
HpySE526I ACGT 2 cut(s) 26, 240
Hsp92I GRCGYC 2 cut(s) 64, 107
Hsp92II CATG 2 cut(s) 459, 472
HspAI GCGC 2 cut(s) 17, 107
KasI GGCGCC 1 cut(s) 106
KroI GCCGGC 1 cut(s) 103
KroNI GCCGGC 1 cut(s) 105
Ksp22I TGATCA 1 cut(s) 216
Kzo9I GATC 4 cut(s) 3, 153, 216, 277
LmnI GCTCC 2 cut(s) 162, 300
LpnPI CCDG 6 cut(s) 74, 102, 117, 123, 164, 207
Lsp1109I GCAGC 2 cut(s) 236, 365
MaeI CTAG 1 cut(s) 360
MaeII ACGT 2 cut(s) 26, 240
MaeIII GTNAC 1 cut(s) 148
MalI GATC 4 cut(s) 5, 155, 218, 279
MboI GATC 4 cut(s) 3, 153, 216, 277
MboII GAAGA 1 cut(s) 262
MhlI GDGCHC 2 cut(s) 174, 297
MluCI AATT 3 cut(s) 308, 364, 412
Mly113I GGCGCC 1 cut(s) 107
MnlI CCTC 7 cut(s) 33, 62, 65, 153, 237, 415, 426
MroNI GCCGGC 1 cut(s) 103
MspA1I CMGCKG 2 cut(s) 48, 227
MspI CCGG 4 cut(s) 61, 104, 110, 194
MwoI GCNNNNNNNGC 2 cut(s) 40, 434
NaeI GCCGGC 1 cut(s) 105
NarI GGCGCC 1 cut(s) 107
NdeII GATC 4 cut(s) 3, 153, 216, 277
NgoMIV GCCGGC 1 cut(s) 103
NlaIII CATG 2 cut(s) 459, 472
NlaIV GGNNCC 2 cut(s) 108, 172
NmuCI GTSAC 1 cut(s) 148
PaqCI CACCTGC 1 cut(s) 169
PdiI GCCGGC 1 cut(s) 105
PkrI GCNGC 3 cut(s) 50, 226, 380
Ple19I CGATCG 1 cut(s) 156
PluTI GGCGCC 1 cut(s) 110
Ppu21I YACGTR 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 297
PspN4I GGNNCC 2 cut(s) 108, 172
PspOMI GGGCCC 1 cut(s) 170
PspPI GGNCC 2 cut(s) 170, 171
PvuI CGATCG 1 cut(s) 156
PvuII CAGCTG 1 cut(s) 227
SacI GAGCTC 1 cut(s) 297
SatI GCNGC 3 cut(s) 49, 225, 379
Sau3AI GATC 4 cut(s) 3, 153, 216, 277
Sau96I GGNCC 2 cut(s) 170, 171
SduI GDGCHC 2 cut(s) 174, 297
SfcI CTRYAG 1 cut(s) 228
SfoI GGCGCC 1 cut(s) 108
Sse9I AATT 3 cut(s) 308, 364, 412
SsiI CCGC 4 cut(s) 32, 48, 208, 281
SspDI GGCGCC 1 cut(s) 106
SspMI CTAG 1 cut(s) 360
SstI GAGCTC 1 cut(s) 297
TaaI ACNGT 2 cut(s) 205, 287
TaiI ACGT 2 cut(s) 29, 243
TaqI TCGA 2 cut(s) 67, 156
TaqII GACCGA 1 cut(s) 392
TasI AATT 3 cut(s) 308, 364, 412
TauI GCSGC 1 cut(s) 51
TscAI CASTG 2 cut(s) 96, 210
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 2 cut(s) 224, 378
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 2 cut(s) 11, 444
TspRI CASTG 2 cut(s) 96, 210
XagI CCTNNNNNAGG 1 cut(s) 121
XapI RAATTY 1 cut(s) 364
XcmI CCANNNNNNNNNTGG 1 cut(s) 96
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.