pycom04g08980

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0017068
Physical Location & Seq
Forward (+)
11264492 .. 11265966
1475 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1434 bp
ATGTCTTCTCATATAGCCTTCAAAACGTTGAAGAAAGTCTTCCCCGCAGAGCCCACATTCATATCGAACCAAGAAACCGCCGAGAAGATTGTCAAAACCCTAATCAAATCAGGCACTCAACCCCTCAAATCAACCTCATCATCTCTGCTATCCAGTCTCAATCCCCACATAACAAACCTTGTCCTCTCAAACCCACTAGTCCTACCACATTCTTGCGTAAAATTATTCAACTTTCTCCAGAAAAATCCATCCCACAAACCCAATCTTAAATCCCACGTTACCCTCCTTTGCAGGCTTTATCAAGCTAGAAAATTTGCCCAGATGAAGATTGTACTGAATGTTATTGTCACGAATGATAATCTTCGATGCCCGGTTTCCGAAATTGCGTCTCTGATTGAGGAGGGGCCTAATGGGATGAAATTTGCTGGGACATTGTGCGATATGCTGTTCCGGGTTTATGCAGAAAACAAAATGGTTGAGGAGGCTATTGGGGTTTTTGATTATGTGGGCAGGAATGGGATTGAGATTAGAGAAAGGTCCTGCATTGTGCTTTTGCTTGCATTGAGGAAATGTGGTCGAGTGGATTCATGTTGGAGGTTCTTCGATCGAATGGTCAAAAACGGTGTTCGAATTACGGTTCTTACATATAACACAATTTTGAAAGCATACATTGGAAGGAAGGATTTTGAGGGCGTTAGTGAGGTTCTGAGGTTGATGGAGAAGGATGGTGTGGGTTATAATGTGGTGACATCCACGCTTTTGATAGATTTGTTTGGTAGTTCTGAGAGGATTGAGGATGCTCAAAAGGTATTTGAGGAAATGCCTGAGAGAGGGATAGAACCAGATTTGTATGTTTATACCTCGATTATTAATTGCTATTGTAAAGCGGGAAATATGGAGAGAGCGTTGTTTCTGCTCGGTGAATGTAATCAAAGGGGGTTTGTACCAAATGAGCATACTTACGGGGCATTGATTAATGGTGCGTGCAAGGCAGGGCAGATGGAGATGGCGGACATCTTGGTGAATGAGATGCAGAGTAAAGGCATTGACGTAAACCAAGTGGTGTTTCATACACTAATGGACGGGTACTGCAAAAAGGGGATGATGGATGAAGCTCTGAAGCTGCGGGATGTCATGGAAAAGAAAGGGTTTCAATTTGATGTGTTTACGTACAGCACTATAGCTAGCGGATTGTATAAATTGAATAGAAATGAGGAGGCAAAGGCTTTGTTGTTCACAGTGGAAGAAAGAGGCGTGACTCCAAATGCTGTGTGTTTCACTATTCTGATAGACATATTCTGCAAGGAAGGAAACTTAGAAGAAGCAAAACGAGTATTCAATGATATGAAAAGGAAGGGAGAGAGACCTAACACTGTCACATACAATGCTCTAATTGACGGGTATAGAGATGGAAGACAAAGGCTTGATGCCTAA

Protein Analysis

478

Amino Acids

53.88

Weight (kDa)

8.52

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 183 - 223 8.7e-08 PPR repeat family
PPR_long PF17177 200 - 303 3e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 201 - 259 1.9e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 213 - 256 1.5e-06 PPR repeat family
PPR_2 PF13041 247 - 291 1.7e-10 PPR repeat family
PPR_3 PF13812 269 - 326 2.6e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 277 - 306 7.9e-09 PPR repeat
PPR_2 PF13041 281 - 330 7.4e-13 PPR repeat family
PPR PF01535 285 - 314 5e-06 PPR repeat
PPR_long PF17177 311 - 385 6.2e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 312 - 344 1.8e-06 PPR repeat
PPR PF01535 320 - 349 1.1e-06 PPR repeat
PPR_2 PF13041 320 - 354 4e-07 PPR repeat family
TPR_24 PF23276 325 - 400 1.5e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 347 - 380 4.3e-09 PPR repeat
PPR_2 PF13041 352 - 400 5.9e-14 PPR repeat family
PPR PF01535 354 - 384 5.6e-08 PPR repeat
PPR_3 PF13812 377 - 434 6.1e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 413 - 468 1.7e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 417 - 450 4.9e-12 PPR repeat
PPR_2 PF13041 421 - 468 8.8e-18 PPR repeat family
PPR PF01535 424 - 453 1.1e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32630
fragaria_vesca FvH4_6g16190
malus_domestica MD04G1094800.v1.1
prunus_persica Prupe.6G234000_v2.0.a1
pyrus_communis pycom04g08980
rosa_chinensis RchiOBHm_Chr3g0470041
rosa_laevigata RLG00000024301
rosa_multiflora Rmu_co8368425.1_g000001 Rmu_sc0017440.1_g000003
rosa_roxburghii Rroxscaffold_6G00411260
rosa_rugosa Rorug03G0106300.1
rosa_samantha Rh3CG170300
rosa_wichuraiana Rw3G014650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 738
AciI CCGC 6 cut(s) 45, 78, 887, 1010, 1126, 1188
AclI AACGTT 1 cut(s) 26
AcsI RAATTY 2 cut(s) 311, 419
AcuI CTGAAG 1 cut(s) 1139
AfaI GTAC 4 cut(s) 333, 945, 1088, 1172
AfiI CCNNNNNNNGG 1 cut(s) 830
AgsI TTSAA 7 cut(s) 22, 31, 229, 661, 1154, 1204, 1339
AhlI ACTAGT 1 cut(s) 196
AjuI GAANNNNNNNTTGG 2 cut(s) 1050, 1082
AluBI AGCT 4 cut(s) 305, 1115, 1123, 1184
AluI AGCT 4 cut(s) 305, 1115, 1123, 1184
Alw26I GTCTC 3 cut(s) 161, 393, 1357
AoxI GGCC 1 cut(s) 404
ApeKI GCWGC 1 cut(s) 1123
ApoI RAATTY 2 cut(s) 311, 419
AseI ATTAAT 2 cut(s) 870, 975
Asp700I GAANNNNTTC 1 cut(s) 38
AspS9I GGNCC 2 cut(s) 404, 537
AsuC2I CCSGG 2 cut(s) 371, 452
AsuHPI GGTGA 3 cut(s) 757, 932, 1033
AsuII TTCGAA 1 cut(s) 628
AsuNHI GCTAGC 1 cut(s) 1184
AvaII GGWCC 1 cut(s) 537
BanII GRGCYC 1 cut(s) 54
BbsI GAAGAC 2 cut(s) 31, 1420
BbvI GCAGC 1 cut(s) 1110
BccI CCATC 7 cut(s) 256, 709, 719, 994, 1000, 1099, 1403
BcnI CCSGG 2 cut(s) 371, 452
BcoDI GTCTC 3 cut(s) 161, 393, 1357
BcuI ACTAGT 1 cut(s) 196
BfaI CTAG 3 cut(s) 197, 306, 1185
BfmI CTRYAG 1 cut(s) 1179
BisI GCNGC 1 cut(s) 1124
BlsI GCNGC 1 cut(s) 1125
Bme1390I CCNGG 2 cut(s) 371, 452
Bme18I GGWCC 1 cut(s) 537
BmgT120I GGNCC 2 cut(s) 404, 537
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 2 cut(s) 371, 452
BmsI GCATC 4 cut(s) 356, 787, 1020, 1417
BmtI GCTAGC 1 cut(s) 1188
BpiI GAAGAC 2 cut(s) 31, 1420
BpmI CTGGAG 1 cut(s) 221
Bpu14I TTCGAA 1 cut(s) 628
BpuMI CCSGG 2 cut(s) 371, 452
BsaAI YACGTR 1 cut(s) 1170
BsaI GGTCTC 1 cut(s) 1357
BsaXI ACNNNNNCTCC 2 cut(s) 890, 920
Bsc4I CCNNNNNNNGG 1 cut(s) 830
Bse1I ACTGG 1 cut(s) 153
BseGI GGATG 8 cut(s) 248, 420, 730, 749, 802, 1107, 1114, 1135
BseLI CCNNNNNNNGG 1 cut(s) 830
BseMII CTCAG 3 cut(s) 698, 774, 816
BseNI ACTGG 1 cut(s) 153
BseRI GAGGAG 3 cut(s) 413, 494, 1229
BseXI GCAGC 1 cut(s) 1110
BseYI CCCAGC 1 cut(s) 425
Bsh1285I CGRYCG 1 cut(s) 607
BshFI GGCC 1 cut(s) 406
BsiEI CGRYCG 1 cut(s) 607
BsiSI CCGG 2 cut(s) 371, 451
BslFI GGGAC 1 cut(s) 442
BslI CCNNNNNNNGG 1 cut(s) 830
BsmAI GTCTC 3 cut(s) 161, 393, 1357
BsmBI CGTCTC 1 cut(s) 393
BsmFI GGGAC 1 cut(s) 442
BsnI GGCC 1 cut(s) 406
Bso31I GGTCTC 1 cut(s) 1357
Bsp119I TTCGAA 1 cut(s) 628
Bsp1286I GDGCHC 1 cut(s) 54
Bsp143I GATC 1 cut(s) 604
BspACI CCGC 6 cut(s) 45, 78, 887, 1010, 1126, 1188
BspANI GGCC 1 cut(s) 406
BspCNI CTCAG 3 cut(s) 699, 775, 817
BspLI GGNNCC 1 cut(s) 405
BspOI GCTAGC 1 cut(s) 1188
BspT104I TTCGAA 1 cut(s) 628
BspTNI GGTCTC 1 cut(s) 1357
BsrI ACTGG 1 cut(s) 153
BssMI GATC 1 cut(s) 604
Bst4CI ACNGT 4 cut(s) 623, 637, 1240, 1375
BstBAI YACGTR 1 cut(s) 1170
BstBI TTCGAA 1 cut(s) 628
BstC8I GCNNGC 4 cut(s) 293, 558, 985, 1186
BstDEI CTNAG 4 cut(s) 707, 783, 825, 1315
BstENI CCTNNNNNAGG 1 cut(s) 828
BstF5I GGATG 8 cut(s) 248, 420, 730, 749, 802, 1107, 1114, 1135
BstKTI GATC 1 cut(s) 607
BstMAI GTCTC 3 cut(s) 161, 393, 1357
BstMBI GATC 1 cut(s) 604
BstMCI CGRYCG 1 cut(s) 607
BstMWI GCNNNNNNNGC 1 cut(s) 989
BstSCI CCNGG 2 cut(s) 369, 450
BstSFI CTRYAG 1 cut(s) 1179
BstSNI TACGTA 1 cut(s) 1170
BstV1I GCAGC 1 cut(s) 1110
BstV2I GAAGAC 2 cut(s) 31, 1420
BsuRI GGCC 1 cut(s) 406
BtsCI GGATG 8 cut(s) 248, 420, 730, 749, 802, 1107, 1114, 1135
BtsIMutI CAGTG 2 cut(s) 1245, 1371
Cac8I GCNNGC 4 cut(s) 293, 558, 985, 1186
Cfr13I GGNCC 2 cut(s) 404, 537
CseI GACGC 1 cut(s) 375
Csp6I GTAC 4 cut(s) 332, 944, 1087, 1171
CviAII CATG 2 cut(s) 588, 1135
CviQI GTAC 4 cut(s) 332, 944, 1087, 1171
DdeI CTNAG 4 cut(s) 707, 783, 825, 1315
DpnI GATC 1 cut(s) 606
DpnII GATC 1 cut(s) 604
EciI GGCGGA 1 cut(s) 1025
Eco105I TACGTA 1 cut(s) 1170
Eco24I GRGCYC 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 1357
Eco47I GGWCC 1 cut(s) 537
Eco57I CTGAAG 1 cut(s) 1139
EcoNI CCTNNNNNAGG 1 cut(s) 828
EcoO109I RGGNCCY 2 cut(s) 404, 537
EcoT38I GRGCYC 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 393
FaeI CATG 2 cut(s) 591, 1138
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FaqI GGGAC 1 cut(s) 442
FatI CATG 2 cut(s) 587, 1134
FauI CCCGC 3 cut(s) 52, 880, 1119
Fnu4HI GCNGC 1 cut(s) 1124
FokI GGATG 8 cut(s) 235, 427, 736, 737, 809, 1114, 1121, 1142
FriOI GRGCYC 1 cut(s) 54
Fsp4HI GCNGC 1 cut(s) 1124
FspBI CTAG 3 cut(s) 197, 306, 1185
GluI GCNGC 1 cut(s) 1124
GsaI CCCAGC 1 cut(s) 429
GsuI CTGGAG 1 cut(s) 221
HaeIII GGCC 1 cut(s) 406
HapII CCGG 2 cut(s) 371, 451
HgaI GACGC 1 cut(s) 375
Hin1II CATG 2 cut(s) 591, 1138
HinfI GANTC 2 cut(s) 584, 1258
HpaII CCGG 2 cut(s) 371, 451
HphI GGTGA 3 cut(s) 757, 932, 1033
Hpy166II GTNNAC 3 cut(s) 1054, 1167, 1236
Hpy188I TCNGA 6 cut(s) 379, 393, 708, 784, 1119, 1287
Hpy188III TCNNGA 2 cut(s) 238, 349
Hpy8I GTNNAC 3 cut(s) 1054, 1167, 1236
HpyAV CCTTC 6 cut(s) 28, 669, 673, 715, 1301, 1348
HpyCH4III ACNGT 4 cut(s) 623, 637, 1240, 1375
HpyCH4IV ACGT 4 cut(s) 26, 276, 1050, 1169
HpyCH4V TGCA 8 cut(s) 291, 461, 543, 560, 987, 1033, 1092, 1302
HpyF10VI GCNNNNNNNGC 1 cut(s) 989
HpyF3I CTNAG 4 cut(s) 707, 783, 825, 1315
HpySE526I ACGT 4 cut(s) 26, 276, 1050, 1169
Hsp92II CATG 2 cut(s) 591, 1138
Kzo9I GATC 1 cut(s) 604
Lsp1109I GCAGC 1 cut(s) 1110
LweI GCATC 4 cut(s) 356, 787, 1020, 1417
MaeI CTAG 3 cut(s) 197, 306, 1185
MaeII ACGT 4 cut(s) 26, 276, 1050, 1169
MaeIII GTNAC 5 cut(s) 277, 346, 745, 1255, 1375
MalI GATC 1 cut(s) 606
MboI GATC 1 cut(s) 604
MboII GAAGA 9 cut(s) 31, 43, 97, 337, 353, 592, 1256, 1331, 1425
MhlI GDGCHC 1 cut(s) 54
MlyI GAGTC 1 cut(s) 1252
MmeI TCCRAC 1 cut(s) 572
MroXI GAANNNNTTC 1 cut(s) 38
MseI TTAA 3 cut(s) 267, 870, 975
MslI CAYNNNNRTG 1 cut(s) 1019
MspI CCGG 2 cut(s) 371, 451
MspR9I CCNGG 2 cut(s) 371, 452
MwoI GCNNNNNNNGC 1 cut(s) 989
NciI CCSGG 2 cut(s) 371, 452
NdeII GATC 1 cut(s) 604
NheI GCTAGC 1 cut(s) 1184
NlaIII CATG 2 cut(s) 591, 1138
NlaIV GGNNCC 1 cut(s) 405
NmeAIII GCCGAG 1 cut(s) 106
NmuCI GTSAC 4 cut(s) 346, 745, 1255, 1375
NspV TTCGAA 1 cut(s) 628
PdmI GAANNNNTTC 1 cut(s) 38
PfeI GAWTC 1 cut(s) 584
PkrI GCNGC 1 cut(s) 1125
Ple19I CGATCG 1 cut(s) 607
PleI GAGTC 1 cut(s) 1252
PpsI GAGTC 1 cut(s) 1252
Ppu21I YACGTR 1 cut(s) 1170
PpuMI RGGWCCY 1 cut(s) 537
PshBI ATTAAT 2 cut(s) 870, 975
PsiI TTATAA 1 cut(s) 738
Psp1406I AACGTT 1 cut(s) 26
Psp5II RGGWCCY 1 cut(s) 537
PspFI CCCAGC 1 cut(s) 425
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 2 cut(s) 404, 537
PspPPI RGGWCCY 1 cut(s) 537
PvuI CGATCG 1 cut(s) 607
RsaI GTAC 4 cut(s) 333, 945, 1088, 1172
RsaNI GTAC 4 cut(s) 332, 944, 1087, 1171
RseI CAYNNNNRTG 1 cut(s) 1019
SaqAI TTAA 3 cut(s) 267, 870, 975
SatI GCNGC 1 cut(s) 1124
Sau3AI GATC 1 cut(s) 604
Sau96I GGNCC 2 cut(s) 404, 537
SchI GAGTC 1 cut(s) 1252
ScrFI CCNGG 2 cut(s) 371, 452
SduI GDGCHC 1 cut(s) 54
SfaNI GCATC 4 cut(s) 356, 787, 1020, 1417
SfcI CTRYAG 1 cut(s) 1179
SfuI TTCGAA 1 cut(s) 628
SinI GGWCC 1 cut(s) 537
SmiMI CAYNNNNRTG 1 cut(s) 1019
SnaBI TACGTA 1 cut(s) 1170
SpeI ACTAGT 1 cut(s) 196
SsiI CCGC 6 cut(s) 45, 78, 887, 1010, 1126, 1188
SspMI CTAG 3 cut(s) 197, 306, 1185
StyD4I CCNGG 2 cut(s) 369, 450
TaaI ACNGT 4 cut(s) 623, 637, 1240, 1375
TaiI ACGT 4 cut(s) 29, 279, 1053, 1172
TaqI TCGA 7 cut(s) 65, 364, 577, 603, 607, 628, 863
TatI WGTACW 1 cut(s) 331
TfiI GAWTC 1 cut(s) 584
Tru1I TTAA 3 cut(s) 267, 870, 975
Tru9I TTAA 3 cut(s) 267, 870, 975
TscAI CASTG 2 cut(s) 1245, 1378
TseFI GTSAC 4 cut(s) 346, 745, 1255, 1375
TseI GCWGC 1 cut(s) 1123
Tsp45I GTSAC 4 cut(s) 346, 745, 1255, 1375
TspDTI ATGAA 7 cut(s) 49, 338, 431, 576, 1058, 1125, 1361
TspRI CASTG 2 cut(s) 1245, 1378
VpaK11BI GGWCC 1 cut(s) 537
VspI ATTAAT 2 cut(s) 870, 975
XagI CCTNNNNNAGG 1 cut(s) 828
XapI RAATTY 2 cut(s) 311, 419
XmnI GAANNNNTTC 1 cut(s) 38
XspI CTAG 3 cut(s) 197, 306, 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.