Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0010990

Physical Location & Seq

Genomic Coordinates Forward (+)

16199991 .. 16206996

Gene Structure 7006 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1989 bp

ATGATTGCTGTCATGGACTTGAATGCCTCGCCAGTACCTGAAGAAGATGAAGATATTTTTGAGAATCATATAGAATACATTGCACCAGAAGAACGCATAGAGAGTGGAGCTGATATAGCACGCCGGGAGCGTGAAGAAAGACGTAAGCGATTGAAACGAGAACGCCCAGATGAAAAACCAGTACATGTTTCTCAACCCCCTGCATACGATTCATATCAAACAAAAAACCCCAAAGTTTATGACAAATCTAGGCTTCCCAATGGTTGGTTGGATTGCCCGGCAGCTGGTCAAGAAATATATTGCATGATACCTTCTAAGGTGCCACTTGGTGAATCCTACAATGATTGTGTTCCTCCTGGTAAAAGATACTCATTTAAACAAGTGATTCATCAGCAAAGAGTTTTGGGAAGAAAACTAGGTTTGGTGATTGATTTGACAAATACTTCTCGCTATTATCTAACATCAGATTTGAAGAAAGAGGGTATTAAGCATGTCAAGATTGCGTGCAAGGGGCGGGATTCTGTGCCAGACAACCTTTCCGTAAATCAGTTTGTATATGAGGTTATACAGTTCCTGTCACGTCAAAAGCATTCGAAGAAATATATTCTTGTGCATTGTACACATGGGCACAATCGCACAGGGTATATGATCATCCACTACCTAATGCGCTCATTACCAATTTCTGTCAGTCAGGCAATAAAATTTTTTGCGGATGCACGTCCTCCAGGAATCTACAAACCTGACTATATTGATGCTTTATACTCATTCTATCATGAAAAAAAGCCTGATATGGTTGTCTGTCCCTCAACTCCTGAATGGAAGACTTCAGAATTTGATCTTAATGGCGAAGCAGTTGCAGACGACGATGACGATGATGGAGGTCCTGCTGCTCCTGTAAATAATGAGAGTCTGGATACAAGTGGAGTAATGACAAATGATGATATCTTGGGCGATGAGATACCTTGGGACCAGCAGGAGGCAATGCGGCAGTTCTGCTATCAAACTCTTAAGCTGGTTAATGTGGGGCCTAGAGGAAATTCACAATTCCCGGGGTCGCACCCAGTTTCTCTTGACAGGGACAACTTACAGCTGTTGAGGCAACGTTATTATTATGCTACATGGAAGGCTGATGGAACACGATATATGATGTTAATAACCATGGATGGGTGTTACTTGGGTTTAGCCGAGAAGACTCACCACTACACGTTATTGGATGGGGAGATGGTAATTGATACCGTGCCTGATTCAAAGAAGCAGGAGAGAAGATACCTGATCTATGATATGATGGCAATCAACCATGTTCCTATCATAGAGCGGCCTTTTTATGAACGGTGGAAGATGCTTGAGAAAGAAGTCATTGAACCCCGGAATCATGAGCGCCACAATATTTACCAAAGTAGGAATCCTTACTATAGATATGACTTGGAACCATTCAGGGTGAGGAGGAAGGACTTTTGGTTGCTCTCTACAGTAAACAAGCTTTTGAAGGAATTTATTCCAAGGCTTTCACATGATGCTGATGGTCTTATCTTTCAGGGTTGGGATGATCCATATGTTCCCCGCACACATGCAGGACTTCTAAAGTGGAAATATTCTCATATGAACTCGGTTGATTTTCTCTTTGAGGTAACCAATGATGATCGGCAGCATCTTTATCTGTTTGAACGTGGAAAGAAGAAACTAATGGATGGAAATAGAGTTGCGTTTAAAGATGGCGAAGACCCCTCATCTTATTCCGGAAAAATAATCGAGTGTTCTTGGGATGGTGAAGAACAAGAATGGGTCCTTATGCGCATCAGGACTGACAAATCAACTCCAAATGATATCAATACCTACAAGAAGGTCATGCGAAGCATAAAAGACAATATCACGCAGGACATCTTGTTGAATGAGATCGATGAGATCATTCGTCTGCCTATGTATGCTGATAGGATACGGAATGATAGCAAAGCAGCCCAGCAGCATGCAAATGCAGCACGAAGGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004484 GO:0004651 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006370 GO:0006396 GO:0006397 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008192 GO:0009452 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010604 GO:0010628 GO:0016070 GO:0016071 GO:0016311 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016791 GO:0016817 GO:0016818 GO:0019222 GO:0034641 GO:0036260 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048518 GO:0050355 GO:0050789 GO:0060255 GO:0065007 GO:0070568 GO:0071704 GO:0090304 GO:0098501 GO:0098507 GO:0098518 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

DSPc mRNA_cap_C mRNA_cap_enzyme

Protein Analysis

663

Amino Acids

77.43

Weight (kDa)

6.42

Isoelectric Point (pI)

50.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DSPc	PF00782	137 - 257	4.1e-16	Dual specificity phosphatase, catalytic domain
mRNA_cap_enzyme	PF01331	353 - 392	2.3e-14	mRNA capping enzyme, catalytic domain
mRNA_cap_enzyme	PF01331	396 - 530	4.1e-46	mRNA capping enzyme, catalytic domain
mRNA_cap_C	PF03919	535 - 629	3.7e-20	mRNA capping enzyme, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010990)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09100 AT3G09100
fragaria_vesca	FvH4_6g12120 FvH4_6g12120 FvH4_6g12120
malus_domestica	MD04G1146600.v1.1 MD12G1159800.v1.1
prunus_persica	Prupe.6G270400_v2.0.a1
pyrus_communis	pycom04g13230 pycom12g15160
rosa_chinensis	RchiOBHm_Chr3g0463921
rosa_laevigata	RLG00000024736
rosa_multiflora	Rmu_sc0000517.1_g000001
rosa_roxburghii	Rroxscaffold_6G00416140
rosa_rugosa	Rorug03G0069200
rosa_samantha	Rh3AG126200 Rh3BG130200 Rh3CG132700 Rh3DG131400
rosa_wichuraiana	Rw3G010650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1793
AccB1I	GGYRCC	1 cut(s)	319
AccB7I	CCANNNNNTGG	1 cut(s)	264
AccBSI	CCGCTC	1 cut(s)	1315
AccIII	TCCGGA	1 cut(s)	1736
AciI	CCGC	5 cut(s)	514, 710, 985, 1315, 1561
AclI	AACGTT	1 cut(s)	1102
AclWI	GGATC	1 cut(s)	1541
AcsI	RAATTY	4 cut(s)	701, 830, 1036, 1490
AcuI	CTGAAG	2 cut(s)	60, 810
AdeI	CACNNNGTG	1 cut(s)	329
AfaI	GTAC	3 cut(s)	36, 183, 619
AfiI	CCNNNNNNNGG	4 cut(s)	264, 284, 976, 1164
AflII	CTTAAG	1 cut(s)	1007
AflIII	ACRYGT	2 cut(s)	184, 1203
AgsI	TTSAA	8 cut(s)	22, 154, 472, 1248, 1361, 1486, 1664, 1888
AjiI	CACGTC	2 cut(s)	581, 719
AjnI	CCWGG	2 cut(s)	355, 724
AluBI	AGCT	5 cut(s)	110, 284, 1012, 1090, 1480
AluI	AGCT	5 cut(s)	110, 284, 1012, 1090, 1480
AlwI	GGATC	1 cut(s)	1541
AlwNI	CAGNNNCTG	2 cut(s)	38, 574
Ama87I	CYCGRG	1 cut(s)	1048
Aor13HI	TCCGGA	1 cut(s)	1736
AoxI	GGCC	2 cut(s)	1025, 1316
ApeKI	GCWGC	6 cut(s)	281, 887, 1645, 1952, 1960, 1973
ApoI	RAATTY	4 cut(s)	701, 830, 1036, 1490
Asp700I	GAANNNNTTC	1 cut(s)	1494
AspLEI	GCGC	3 cut(s)	669, 1380, 1794
AspS9I	GGNCC	4 cut(s)	881, 967, 1025, 1783
AsuC2I	CCSGG	5 cut(s)	125, 278, 1049, 1050, 1366
AsuHPI	GGTGA	5 cut(s)	341, 436, 1187, 1450, 1778
AsuII	TTCGAA	1 cut(s)	593
AvaI	CYCGRG	1 cut(s)	1048
AvaII	GGWCC	3 cut(s)	881, 967, 1783
BaeGI	GKGCMC	1 cut(s)	630
BanI	GGYRCC	1 cut(s)	319
BbsI	GAAGAC	3 cut(s)	827, 1196, 1725
BbvI	GCAGC	6 cut(s)	293, 874, 1657, 1964, 1972, 1985
BcgI	CGANNNNNNTGC	2 cut(s)	836, 870
BciT130I	CCWGG	2 cut(s)	357, 726
BciVI	GTATCC	2 cut(s)	907, 1926
BclI	TGATCA	1 cut(s)	648
BcnI	CCSGG	5 cut(s)	125, 278, 1049, 1050, 1366
BfaI	CTAG	3 cut(s)	249, 416, 1029
BfmI	CTRYAG	2 cut(s)	1411, 1467
BfoI	RGCGCY	1 cut(s)	1381
BfrI	CTTAAG	1 cut(s)	1007
BfuI	GTATCC	2 cut(s)	907, 1926
BisI	GCNGC	8 cut(s)	282, 888, 986, 1316, 1646, 1953, 1961, 1974
BlsI	GCNGC	8 cut(s)	283, 889, 987, 1317, 1647, 1954, 1962, 1975
Bme1390I	CCNGG	7 cut(s)	125, 278, 357, 726, 1049, 1050, 1366
Bme18I	GGWCC	3 cut(s)	881, 967, 1783
BmeT110I	CYCGRG	1 cut(s)	1048
BmgBI	CACGTC	2 cut(s)	581, 719
BmgT120I	GGNCC	4 cut(s)	881, 967, 1025, 1783
BmiI	GGNNCC	5 cut(s)	321, 968, 1026, 1428, 1784
BmrFI	CCNGG	7 cut(s)	125, 278, 357, 726, 1049, 1050, 1366
BmrI	ACTGGG	1 cut(s)	1055
BmsI	GCATC	6 cut(s)	703, 742, 1329, 1504, 1657, 1803
BmuI	ACTGGG	1 cut(s)	1055
BpiI	GAAGAC	3 cut(s)	827, 1196, 1725
BpmI	CTGGAG	1 cut(s)	708
Bpu14I	TTCGAA	1 cut(s)	593
BpuEI	CTTGAG	1 cut(s)	1364
BpuMI	CCSGG	5 cut(s)	125, 278, 1049, 1050, 1366
Bsa29I	ATCGAT	1 cut(s)	1896
BsaBI	GATNNNNATC	2 cut(s)	213, 1289
BsaJI	CCNNGG	6 cut(s)	962, 1048, 1049, 1158, 1364, 1499
BsaWI	WCCGGW	1 cut(s)	1736
Bsc4I	CCNNNNNNNGG	4 cut(s)	264, 284, 976, 1164
Bse1I	ACTGG	3 cut(s)	32, 179, 1061
Bse3DI	GCAATG	2 cut(s)	78, 987
Bse8I	GATNNNNATC	2 cut(s)	213, 1289
BseAI	TCCGGA	1 cut(s)	1736
BseBI	CCWGG	2 cut(s)	357, 726
BseCI	ATCGAT	1 cut(s)	1896
BseDI	CCNNGG	6 cut(s)	962, 1048, 1049, 1158, 1364, 1499
BseGI	GGATG	7 cut(s)	651, 718, 1168, 1219, 1549, 1693, 1768
BseJI	GATNNNNATC	2 cut(s)	213, 1289
BseLI	CCNNNNNNNGG	4 cut(s)	264, 284, 976, 1164
BseMI	GCAATG	2 cut(s)	78, 987
BseNI	ACTGG	3 cut(s)	32, 179, 1061
BseRI	GAGGAG	1 cut(s)	1456
BseSI	GKGCMC	1 cut(s)	630
BseXI	GCAGC	6 cut(s)	293, 874, 1657, 1964, 1972, 1985
BseYI	CCCAGC	1 cut(s)	1956
BshFI	GGCC	2 cut(s)	1027, 1318
BshNI	GGYRCC	1 cut(s)	319
BshVI	ATCGAT	1 cut(s)	1896
BsiHKCI	CYCGRG	1 cut(s)	1048
BsiSI	CCGG	5 cut(s)	124, 278, 1049, 1366, 1737
BslFI	GGGAC	3 cut(s)	786, 980, 1091
BslI	CCNNNNNNNGG	4 cut(s)	264, 284, 976, 1164
BsmFI	GGGAC	3 cut(s)	786, 980, 1091
BsmI	GAATGC	2 cut(s)	28, 589
BsnI	GGCC	2 cut(s)	1027, 1318
BsoBI	CYCGRG	1 cut(s)	1048
Bsp119I	TTCGAA	1 cut(s)	593
Bsp1286I	GDGCHC	1 cut(s)	630
Bsp13I	TCCGGA	1 cut(s)	1736
Bsp1407I	TGTACA	1 cut(s)	617
Bsp143I	GATC	7 cut(s)	648, 835, 1272, 1546, 1639, 1893, 1902
Bsp19I	CCATGG	1 cut(s)	1158
BspACI	CCGC	5 cut(s)	514, 710, 985, 1315, 1561
BspANI	GGCC	2 cut(s)	1027, 1318
BspDI	ATCGAT	1 cut(s)	1896
BspEI	TCCGGA	1 cut(s)	1736
BspHI	TCATGA	2 cut(s)	772, 1372
BspLI	GGNNCC	5 cut(s)	321, 968, 1026, 1428, 1784
BspPI	GGATC	1 cut(s)	1541
BspT104I	TTCGAA	1 cut(s)	593
BspT107I	GGYRCC	1 cut(s)	319
BspTI	CTTAAG	1 cut(s)	1007
BsrBI	CCGCTC	1 cut(s)	1315
BsrDI	GCAATG	2 cut(s)	78, 987
BsrGI	TGTACA	1 cut(s)	617
BsrI	ACTGG	3 cut(s)	32, 179, 1061
BssECI	CCNNGG	6 cut(s)	962, 1048, 1049, 1158, 1364, 1499
BssMI	GATC	7 cut(s)	648, 835, 1272, 1546, 1639, 1893, 1902
BssT1I	CCWWGG	3 cut(s)	962, 1158, 1499
Bst2UI	CCWGG	2 cut(s)	357, 726
Bst4CI	ACNGT	4 cut(s)	570, 1237, 1332, 1471
BstAFI	CTTAAG	1 cut(s)	1007
BstAUI	TGTACA	1 cut(s)	617
BstBI	TTCGAA	1 cut(s)	593
BstC8I	GCNNGC	3 cut(s)	121, 505, 1965
BstDEI	CTNAG	1 cut(s)	315
BstDSI	CCRYGG	1 cut(s)	1158
BstEII	GGTNACC	1 cut(s)	1627
BstF5I	GGATG	7 cut(s)	651, 718, 1168, 1219, 1549, 1693, 1768
BstH2I	RGCGCY	1 cut(s)	1381
BstHHI	GCGC	3 cut(s)	669, 1380, 1794
BstKTI	GATC	7 cut(s)	651, 838, 1275, 1549, 1642, 1896, 1905
BstMBI	GATC	7 cut(s)	648, 835, 1272, 1546, 1639, 1893, 1902
BstMWI	GCNNNNNNNGC	3 cut(s)	116, 1096, 1973
BstNI	CCWGG	2 cut(s)	357, 726
BstNSI	RCATGY	4 cut(s)	188, 494, 1571, 1967
BstPI	GGTNACC	1 cut(s)	1627
BstSCI	CCNGG	7 cut(s)	123, 276, 355, 724, 1047, 1048, 1364
BstSFI	CTRYAG	2 cut(s)	1411, 1467
BstSLI	GKGCMC	1 cut(s)	630
BstV1I	GCAGC	6 cut(s)	293, 874, 1657, 1964, 1972, 1985
BstV2I	GAAGAC	3 cut(s)	827, 1196, 1725
Bsu15I	ATCGAT	1 cut(s)	1896
BsuI	GTATCC	2 cut(s)	907, 1926
BsuRI	GGCC	2 cut(s)	1027, 1318
BsuTUI	ATCGAT	1 cut(s)	1896
BtgI	CCRYGG	1 cut(s)	1158
BtgZI	GCGATG	1 cut(s)	966
BtrI	CACGTC	2 cut(s)	581, 719
BtsCI	GGATG	7 cut(s)	651, 718, 1168, 1219, 1549, 1693, 1768
Cac8I	GCNNGC	3 cut(s)	121, 505, 1965
CaiI	CAGNNNCTG	2 cut(s)	38, 574
CciI	TCATGA	2 cut(s)	772, 1372
CfoI	GCGC	3 cut(s)	669, 1380, 1794
Cfr13I	GGNCC	4 cut(s)	881, 967, 1025, 1783
Cfr9I	CCCGGG	1 cut(s)	1048
ClaI	ATCGAT	1 cut(s)	1896
Csp6I	GTAC	3 cut(s)	35, 182, 618
CviQI	GTAC	3 cut(s)	35, 182, 618
DdeI	CTNAG	1 cut(s)	315
DpnI	GATC	7 cut(s)	650, 837, 1274, 1548, 1641, 1895, 1904
DpnII	GATC	7 cut(s)	648, 835, 1272, 1546, 1639, 1893, 1902
DraI	TTTAAA	2 cut(s)	376, 1708
DraIII	CACNNNGTG	1 cut(s)	329
Eco130I	CCWWGG	3 cut(s)	962, 1158, 1499
Eco32I	GATATC	2 cut(s)	943, 1825
Eco47I	GGWCC	3 cut(s)	881, 967, 1783
Eco57I	CTGAAG	2 cut(s)	60, 810
Eco88I	CYCGRG	1 cut(s)	1048
Eco91I	GGTNACC	1 cut(s)	1627
EcoO109I	RGGNCCY	3 cut(s)	881, 1025, 1783
EcoO65I	GGTNACC	1 cut(s)	1627
EcoRII	CCWGG	2 cut(s)	355, 724
EcoRV	GATATC	2 cut(s)	943, 1825
EcoT14I	CCWWGG	3 cut(s)	962, 1158, 1499
ErhI	CCWWGG	3 cut(s)	962, 1158, 1499
FaqI	GGGAC	3 cut(s)	786, 980, 1091
FauI	CCCGC	2 cut(s)	507, 1568
FauNDI	CATATG	2 cut(s)	1552, 1599
FbaI	TGATCA	1 cut(s)	648
Fnu4HI	GCNGC	8 cut(s)	282, 888, 986, 1316, 1646, 1953, 1961, 1974
FokI	GGATG	7 cut(s)	638, 725, 1175, 1226, 1556, 1700, 1775
Fsp4HI	GCNGC	8 cut(s)	282, 888, 986, 1316, 1646, 1953, 1961, 1974
FspAI	RTGCGCAY	1 cut(s)	1793
FspBI	CTAG	3 cut(s)	249, 416, 1029
FspI	TGCGCA	1 cut(s)	1793
GlaI	GCGC	3 cut(s)	668, 1379, 1793
GluI	GCNGC	8 cut(s)	282, 888, 986, 1316, 1646, 1953, 1961, 1974
GsaI	CCCAGC	1 cut(s)	1960
GsuI	CTGGAG	1 cut(s)	708
HaeII	RGCGCY	1 cut(s)	1381
HaeIII	GGCC	2 cut(s)	1027, 1318
HapII	CCGG	5 cut(s)	124, 278, 1049, 1366, 1737
HhaI	GCGC	3 cut(s)	669, 1380, 1794
Hin6I	GCGC	3 cut(s)	667, 1378, 1792
HinP1I	GCGC	3 cut(s)	667, 1378, 1792
HindIII	AAGCTT	1 cut(s)	1478
HpaII	CCGG	5 cut(s)	124, 278, 1049, 1366, 1737
HphI	GGTGA	5 cut(s)	341, 436, 1187, 1450, 1778
Hpy166II	GTNNAC	2 cut(s)	620, 1474
Hpy188I	TCNGA	2 cut(s)	466, 829
Hpy188III	TCNNGA	9 cut(s)	290, 496, 773, 812, 911, 1070, 1373, 1737, 1798
Hpy8I	GTNNAC	2 cut(s)	620, 1474
Hpy99I	CGWCG	1 cut(s)	866
HpyAV	CCTTC	6 cut(s)	321, 1117, 1441, 1480, 1834, 1974
HpyCH4III	ACNGT	4 cut(s)	570, 1237, 1332, 1471
HpyCH4IV	ACGT	6 cut(s)	142, 580, 718, 1102, 1205, 1666
HpyF10VI	GCNNNNNNNGC	3 cut(s)	116, 1096, 1973
HpyF3I	CTNAG	1 cut(s)	315
HpySE526I	ACGT	6 cut(s)	142, 580, 718, 1102, 1205, 1666
HspAI	GCGC	3 cut(s)	667, 1378, 1792
Kpn2I	TCCGGA	1 cut(s)	1736
Ksp22I	TGATCA	1 cut(s)	648
Kzo9I	GATC	7 cut(s)	648, 835, 1272, 1546, 1639, 1893, 1902
LmnI	GCTCC	3 cut(s)	107, 127, 895
Lsp1109I	GCAGC	6 cut(s)	293, 874, 1657, 1964, 1972, 1985
LweI	GCATC	6 cut(s)	703, 742, 1329, 1504, 1657, 1803
MaeI	CTAG	3 cut(s)	249, 416, 1029
MaeII	ACGT	6 cut(s)	142, 580, 718, 1102, 1205, 1666
MaeIII	GTNAC	3 cut(s)	576, 1169, 1627
MalI	GATC	7 cut(s)	650, 837, 1274, 1548, 1641, 1895, 1904
MbiI	CCGCTC	1 cut(s)	1315
MboI	GATC	7 cut(s)	648, 835, 1272, 1546, 1639, 1893, 1902
MhlI	GDGCHC	1 cut(s)	630
MluCI	AATT	7 cut(s)	678, 701, 830, 1036, 1043, 1227, 1490
MlyI	GAGTC	2 cut(s)	916, 1186
MmeI	TCCRAC	1 cut(s)	249
MroI	TCCGGA	1 cut(s)	1736
MroXI	GAANNNNTTC	1 cut(s)	1494
MseI	TTAA	7 cut(s)	375, 486, 840, 1008, 1017, 1151, 1707
MslI	CAYNNNNRTG	1 cut(s)	1968
MspA1I	CMGCKG	2 cut(s)	284, 1090
MspCI	CTTAAG	1 cut(s)	1007
MspI	CCGG	5 cut(s)	124, 278, 1049, 1366, 1737
MspR9I	CCNGG	7 cut(s)	125, 278, 357, 726, 1049, 1050, 1366
Mva1269I	GAATGC	2 cut(s)	28, 589
MvaI	CCWGG	2 cut(s)	357, 726
MwoI	GCNNNNNNNGC	3 cut(s)	116, 1096, 1973
NciI	CCSGG	5 cut(s)	125, 278, 1049, 1050, 1366
NcoI	CCATGG	1 cut(s)	1158
NdeI	CATATG	2 cut(s)	1552, 1599
NdeII	GATC	7 cut(s)	648, 835, 1272, 1546, 1639, 1893, 1902
NlaIV	GGNNCC	5 cut(s)	321, 968, 1026, 1428, 1784
NmeAIII	GCCGAG	1 cut(s)	1210
NmuCI	GTSAC	1 cut(s)	576
NsbI	TGCGCA	1 cut(s)	1793
NspI	RCATGY	4 cut(s)	188, 494, 1571, 1967
NspV	TTCGAA	1 cut(s)	593
PaeI	GCATGC	1 cut(s)	1967
PagI	TCATGA	2 cut(s)	772, 1372
PciI	ACATGT	1 cut(s)	184
PcsI	WCGNNNNNNNCGW	2 cut(s)	127, 867
PctI	GAATGC	2 cut(s)	28, 589
PdmI	GAANNNNTTC	1 cut(s)	1494
PfeI	GAWTC	9 cut(s)	64, 209, 332, 385, 518, 729, 1244, 1369, 1402
PflMI	CCANNNNNTGG	1 cut(s)	264
PfoI	TCCNGGA	1 cut(s)	724
PkrI	GCNGC	8 cut(s)	283, 889, 987, 1317, 1647, 1954, 1962, 1975
PleI	GAGTC	2 cut(s)	915, 1186
PpsI	GAGTC	2 cut(s)	915, 1186
PpuMI	RGGWCCY	2 cut(s)	881, 1783
PscI	ACATGT	1 cut(s)	184
Psp1406I	AACGTT	1 cut(s)	1102
Psp5II	RGGWCCY	2 cut(s)	881, 1783
Psp6I	CCWGG	2 cut(s)	355, 724
PspEI	GGTNACC	1 cut(s)	1627
PspFI	CCCAGC	1 cut(s)	1956
PspGI	CCWGG	2 cut(s)	355, 724
PspN4I	GGNNCC	5 cut(s)	321, 968, 1026, 1428, 1784
PspPI	GGNCC	4 cut(s)	881, 967, 1025, 1783
PspPPI	RGGWCCY	2 cut(s)	881, 1783
PstNI	CAGNNNCTG	2 cut(s)	38, 574
PvuII	CAGCTG	2 cut(s)	284, 1090
RsaI	GTAC	3 cut(s)	36, 183, 619
RsaNI	GTAC	3 cut(s)	35, 182, 618
RseI	CAYNNNNRTG	1 cut(s)	1968
SaqAI	TTAA	7 cut(s)	375, 486, 840, 1008, 1017, 1151, 1707
SatI	GCNGC	8 cut(s)	282, 888, 986, 1316, 1646, 1953, 1961, 1974
Sau3AI	GATC	7 cut(s)	648, 835, 1272, 1546, 1639, 1893, 1902
Sau96I	GGNCC	4 cut(s)	881, 967, 1025, 1783
SchI	GAGTC	2 cut(s)	916, 1186
ScrFI	CCNGG	7 cut(s)	125, 278, 357, 726, 1049, 1050, 1366
SduI	GDGCHC	1 cut(s)	630
SfaNI	GCATC	6 cut(s)	703, 742, 1329, 1504, 1657, 1803
SfcI	CTRYAG	2 cut(s)	1411, 1467
SfuI	TTCGAA	1 cut(s)	593
SinI	GGWCC	3 cut(s)	881, 967, 1783
SmaI	CCCGGG	1 cut(s)	1050
SmiMI	CAYNNNNRTG	1 cut(s)	1968
SmlI	CTYRAG	2 cut(s)	1007, 1343
SmoI	CTYRAG	2 cut(s)	1007, 1343
SphI	GCATGC	1 cut(s)	1967
Sse9I	AATT	7 cut(s)	678, 701, 830, 1036, 1043, 1227, 1490
SsiI	CCGC	5 cut(s)	514, 710, 985, 1315, 1561
SspI	AATATT	2 cut(s)	1387, 1592
SspMI	CTAG	3 cut(s)	249, 416, 1029
StyD4I	CCNGG	7 cut(s)	123, 276, 355, 724, 1047, 1048, 1364
StyI	CCWWGG	3 cut(s)	962, 1158, 1499
TaaI	ACNGT	4 cut(s)	570, 1237, 1332, 1471
TaiI	ACGT	6 cut(s)	145, 583, 721, 1105, 1208, 1669
TaqI	TCGA	3 cut(s)	593, 1749, 1896
TasI	AATT	7 cut(s)	678, 701, 830, 1036, 1043, 1227, 1490
TatI	WGTACW	2 cut(s)	181, 617
TauI	GCSGC	2 cut(s)	988, 1318
TfiI	GAWTC	9 cut(s)	64, 209, 332, 385, 518, 729, 1244, 1369, 1402
Tru1I	TTAA	7 cut(s)	375, 486, 840, 1008, 1017, 1151, 1707
Tru9I	TTAA	7 cut(s)	375, 486, 840, 1008, 1017, 1151, 1707
TseFI	GTSAC	1 cut(s)	576
TseI	GCWGC	6 cut(s)	281, 887, 1645, 1952, 1960, 1973
Tsp45I	GTSAC	1 cut(s)	576
TspDTI	ATGAA	7 cut(s)	63, 186, 201, 377, 789, 1341, 1616
TspGWI	ACGGA	2 cut(s)	529, 1951
TspMI	CCCGGG	1 cut(s)	1048
Van91I	CCANNNNNTGG	1 cut(s)	264
Vha464I	CTTAAG	1 cut(s)	1007
VpaK11BI	GGWCC	3 cut(s)	881, 967, 1783
XapI	RAATTY	4 cut(s)	701, 830, 1036, 1490
XceI	RCATGY	4 cut(s)	188, 494, 1571, 1967
XcmI	CCANNNNNNNNNTGG	1 cut(s)	265
XmaI	CCCGGG	1 cut(s)	1048
XmnI	GAANNNNTTC	1 cut(s)	1494
XspI	CTAG	3 cut(s)	249, 416, 1029

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.