pycom04g17860

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0010008
Physical Location & Seq
Reverse (-)
20082456 .. 20086629
4174 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3357 bp
ATGAACAACGAAATCGGATTGGACGGGAAATCGGATGCCAGTGAAGAGGCCGCGGAAGCTAGAGCTAGGGTTTCACCAATGGAGCTCGATTCGGGGAGTGAGGCTGGCGGAAGATCTGAGGAGGATGGGGCTAGGGTTAGTACGGAGAGTGAAGGTGGAAATGTGGATAAAGTGATGGAATCGAAGGGTTCTGGAATTCAGGCGGATAGAACTAGGGTGGAGATTTCATTCAATGATGATGAAGACGATGTTTTCGACATTTCGCGGGTCGAAATTGAGGACGATTCTGATAAATCTGAGGCTCACAATGGTGAGCACCCGTCTTTGTTGTCTGAGTTTGATGAGTTTGTAGCTAACGAGAAGAGTGGAATGGCTCTCGGGACGAAGAGGGCGCTGAGCTACGGGTTCGAAGTGGGTGACATGGTGTGGGGGAAGGTAAAATCTCATCCGTGGTGGCCGGGACACATATTTAACGAGGAGTTGGCAACGTCTCAGGTGCGGCGGACACGGAGGGAAGGTCACGTCTTGGTTGCTTTCTTTGGTGATAATAGTTACGGATGGTTCGACCCGGCTGAACTCATACCATTTAATCCCCACTACGCTGAGAAATCTCGGCAGACGAATCACAGGGGTTTTCTGAAGGCTGTGGAGGAGGCTGTCGATGAGGCGAATCGTAGGTGTGAACTTGGTCTGGTTTGCAAATGTAGGAATGCCTATAATTTTCGGAAGACAAGCGTGCAAGGGTACTTTGTTGTTGATGTGCCGGATTATGAGCCAGGAGCAGTGTATTCGGAGAATCAGATAAGGAAGGCGAGGGATAGTTTTAAGCCGATTGAGATGCTGTCTTTTGTAAAGCAGTTGGCTTTGTCAACACACTGTGATGATCAGAAGAGCCTCAGCTTTAATAAGAGCAAGGCTACTGCTTTTTCTTTTCGCAAGGCTGTTTTTGAAGAGTATGATGAAACGTACGCTCAGGCATTTGGTGCACATCCAGGACGCCCTTCTCGTAGTCTAGTTCCAGCTCCAACTCGAGCTGCTTTGAGTGGTCCTTTGGTGATTGCTGAGGTTCTGGGAGGGCAGAAGAGTGCCACAAAACCGATGAAAGTCAAGGACCATTCAAAGAGAGACAAATACCTATTTAAGCGGAGAGATGAACCTGGGAACTCGAAAACCCATCAAACTAGCCAGGGCCAAGCTAGTTCCTCAGCCCCATCTGCCATCTTGGAGGGGTCAATAGCAGTAGAAGATGAGAATTATATGTTACAAAAGAGGGCTCCAGATGTATCTGGAAAAGAAGCTGCAATCATTGATCAGGCAACAAATAGCTCTCTCATCCCCCAAGATGTTACCGTTGATGCAAAGCCATCTCTTGCAAAGGGAAAGGGGGCTCTACAGGAAGTGAAAGAGGGAGATGGAGATGTAGGTCCTACAGCCACAGGATATGTAGATTTATTGGGGGATGGGACCAAGCAACGTACAATAGATGGTACATCACAACCTTTAAAGCAGGAAGGTGAGGGTGTATTAGAGTTTAAATATGAAGAGAGTGAAAAATTGTCTGGATCATATGAAAAATTTCAACAACCTTCGAGTTCTTTGAAAAAAGTGGAAGTAGGCTATGAACGTGGTGTTGGAGATCCTTTGCCAATTGAGGCAAAGAGTTTAGGTGGGAAGAAAGCAGCTGGTGGAGTGAAAAAACCGAAGGTTCTTAAACGGACTGCAGAGGACTTGAACATTGATGACTGTATGATGGGGGATAAAAAGAAAAAGAAAAGGAAGAAACAGTTAGATGCGGAAGCTAGCATCAGAAATCAACAGAAGCCTTTGATTTCTGGAAAGGTACTGCCCTCAGGGAGTAAAGCGGCAGGAAATGCCAATCATGTAGGTTTGGCTGCAAGAGAGGATACCCAGGTTGAACATAAGAAGGATGTCACTTCCAGTAATAACTTGTCTGAATCTGGTGGAAAATTACCGTTTCGTGGCTTGGAAAATGTGCAGCTTGATCTACCACAACTGGTACGCGATTTGCAAGCCCTTGCCCTTGATCCTTTTCACGGTATTGAGACAAACAGCCCTGCAACTGTTCAGCAGTTCTTTCTGCACTTCAGGTCCCTTGTCTACCAGAAAAGTTTGGTTCTGTCACCACCCTCAGAGATTGAACCTGTTGAAGTTCGCTCCTCTAAATCTTCATCTGGTGTGAAAGCCCCTGATATATCTCCTACTGAGCAAGTTAGAGATTTATCGTCCTCAAAGGCAGCAAAACCCGTGTTCAGATCCGACGATCCTACCATAGCTGGGCGGAAACGTGCTCCATCTGACCGTCAAGGAGATCTTGCAGCTAAGAGGTACAAGAAAATTACTGATATAAGAACATTGGCTGCAGAAAAGAAGGCTATTCAGAGACCTCCTATAGACTCCAAGCGTATAGAGGCGAAAGAATCAGCAACACCGGTTCTGCGGAAGTCACTCAAGCCAGGTTTTGCAAAGAAAACGGACCCTGCATCAAAGGCAGTTGAGCCTACAATGCTGGTGTTGAAGTTCCCTCCCAAAATCTCTCTCCCATCGCTTGCAGAACTGAAGGCAAAGTTTGCCCGATTTGGGCCTATGGATCAGTCCGGTCTCCGTGTCTTTTGGAAGTCCTCGACATGCCGGGTTGTCTTTCTGTACAAATCTGATGCACAAGCAGCATTTAAGTTTGCTACTGCAAACAGCTCTCTTTTCGGCAACTTTAGTGTGAGGTGTCAAATTCGAGAAGTGGGCGGTCCTGAAGCTCCAGAGTTAGGCAAGGGTGACAATCCCAGGGAGACGCCACGGGCCAAGGATCCATCGGTTATGCAATCACCAGCATTGGCCTCGGCACTCAGACAGCAGCAGCAGCAGCAGGCTCAACAGAGTGGAGTGCAGCTGAAGTCGATTCTGAAGAAGTATTCAGGCGAGGAGTCGGCAGGAGGGCAGGTAACCGGTGGGAATGGAAATAGTAAAGGGACTGCACGTGTAAAATTCATGTTGGGTGGGGAAGAAACTAGTAGGACTACTACTTCTGATCAATTCATGATGCCTGGTAATAGAAATAACAACTTCAACAAGAACAACAGTGCTAGTTTTGTGGACGTCGACGGTGCACCGTCTTCTACTACTTCCACTGCTACGGATTTTAATACAACTAGGAACTTTCAAAAGGTCAATCCTCCTCCAACTTTTTCATCACCATTGCCTCCTCTCCTCCCTAATCCTCCCGGCCCACCTCCACAATATGCCAAACCCCCACCCCATAGCAATTTTCCGCAGCATTCCGAAATGGCACCACCCAGAAATACTAGTCACAATCTTAACGCACCACCCACAAGCACGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko05206 map00270 map01100 map05206
Pfam Domains
Protein Families
PWWP

Protein Analysis

1119

Amino Acids

121.78

Weight (kDa)

8.82

Isoelectric Point (pI)

45.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 139 - 223 1.8e-19 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 3114
Acc36I ACCTGC 1 cut(s) 2944
AccB1I GGYRCC 1 cut(s) 3301
AccI GTMKAC 2 cut(s) 2118, 3114
AccII CGCG 3 cut(s) 53, 265, 2022
AclWI GGATC 8 cut(s) 1570, 1631, 2039, 2268, 2276, 2616, 2816, 2829
AcoI YGGCCR 1 cut(s) 455
AcsI RAATTY 4 cut(s) 195, 1574, 2745, 2999
AcuI CTGAAG 6 cut(s) 659, 2089, 2597, 2787, 2927, 2939
AcvI CACGTG 1 cut(s) 2993
AcyI GRCGYC 3 cut(s) 997, 2807, 3111
AdeI CACNNNGTG 1 cut(s) 878
AfaI GTAC 9 cut(s) 142, 746, 968, 1477, 1489, 1842, 2019, 2348, 2666
AfiI CCNNNNNNNGG 5 cut(s) 1979, 2054, 2295, 2598, 2780
AflIII ACRYGT 1 cut(s) 2992
AgeI ACCGGT 2 cut(s) 2449, 2960
AhlI ACTAGT 2 cut(s) 3023, 3317
AjiI CACGTC 1 cut(s) 523
AjnI CCWGG 8 cut(s) 775, 991, 1156, 1185, 1908, 2473, 2798, 3058
AjuI GAANNNNNNNTTGG 2 cut(s) 1614, 1646
AleI CACNNNNGTG 1 cut(s) 309
Alw21I GWGCWC 5 cut(s) 87, 318, 988, 2311, 3124
Alw26I GTCTC 6 cut(s) 495, 1119, 2057, 2395, 2624, 2798
Alw44I GTGCAC 2 cut(s) 984, 3120
AlwI GGATC 8 cut(s) 1570, 1631, 2039, 2268, 2276, 2616, 2816, 2829
Ama87I CYCGRG 2 cut(s) 377, 1029
AoxI GGCC 7 cut(s) 48, 455, 1189, 2600, 2814, 2850, 3238
ApaLI GTGCAC 2 cut(s) 984, 3120
ApoI RAATTY 4 cut(s) 195, 1574, 2745, 2999
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
AsiGI ACCGGT 2 cut(s) 2449, 2960
Asp700I GAANNNNTTC 1 cut(s) 1575
AspLEI GCGC 1 cut(s) 394
AsuC2I CCSGG 4 cut(s) 459, 569, 2651, 3237
AsuII TTCGAA 1 cut(s) 408
AsuNHI GCTAGC 1 cut(s) 1799
AvaI CYCGRG 2 cut(s) 377, 1029
AvaII GGWCC 7 cut(s) 1046, 1111, 1424, 1464, 2109, 2494, 2762
AxyI CCTNAGG 1 cut(s) 1849
BaeGI GKGCMC 2 cut(s) 988, 3124
BaeI ACNNNNGTAYC 2 cut(s) 1896, 1929
BamHI GGATCC 1 cut(s) 2821
BanI GGYRCC 1 cut(s) 3301
BanII GRGCYC 3 cut(s) 87, 1276, 1390
BbrPI CACGTG 1 cut(s) 2993
BbsI GAAGAC 3 cut(s) 249, 734, 3120
Bbv12I GWGCWC 5 cut(s) 87, 318, 988, 2311, 3124
BbvCI CCTCAGC 3 cut(s) 896, 1062, 1204
BcgI CGANNNNNNTGC 6 cut(s) 771, 805, 820, 854, 2835, 2869
BciT130I CCWGG 8 cut(s) 777, 993, 1158, 1187, 1910, 2475, 2800, 3060
BciVI GTATCC 1 cut(s) 1897
BclI TGATCA 3 cut(s) 883, 1309, 3043
BcnI CCSGG 4 cut(s) 459, 569, 2651, 3237
BcoDI GTCTC 6 cut(s) 495, 1119, 2057, 2395, 2624, 2798
BcuI ACTAGT 2 cut(s) 3023, 3317
BfmI CTRYAG 5 cut(s) 1391, 1428, 1719, 2379, 2409
BfoI RGCGCY 1 cut(s) 395
BfuAI ACCTGC 1 cut(s) 2944
BfuI GTATCC 1 cut(s) 1897
BglII AGATCT 2 cut(s) 113, 2329
BlpI GCTNAGC 1 cut(s) 395
Bme18I GGWCC 7 cut(s) 1046, 1111, 1424, 1464, 2109, 2494, 2762
BmeT110I CYCGRG 2 cut(s) 377, 1029
BmgBI CACGTC 1 cut(s) 523
BmiI GGNNCC 6 cut(s) 1275, 1465, 2111, 2496, 2823, 3303
BmsI GCATC 8 cut(s) 25, 828, 1345, 1780, 1812, 2510, 2665, 3045
BmtI GCTAGC 1 cut(s) 1803
BpiI GAAGAC 3 cut(s) 249, 734, 3120
BpmI CTGGAG 2 cut(s) 1260, 2757
Bpu10I CCTNAGC 4 cut(s) 896, 972, 1062, 1204
Bpu1102I GCTNAGC 1 cut(s) 395
Bpu14I TTCGAA 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 2453
BpuMI CCSGG 4 cut(s) 459, 569, 2651, 3237
BsaAI YACGTR 1 cut(s) 2993
BsaHI GRCGYC 3 cut(s) 997, 2807, 3111
BsaI GGTCTC 2 cut(s) 2395, 2624
BsaWI WCCGGW 3 cut(s) 2449, 2615, 2960
BsaXI ACNNNNNCTCC 4 cut(s) 502, 532, 2940, 2970
Bsc4I CCNNNNNNNGG 5 cut(s) 1979, 2054, 2295, 2598, 2780
Bse118I RCCGGY 2 cut(s) 2449, 2960
Bse1I ACTGG 3 cut(s) 39, 1938, 2019
Bse21I CCTNAGG 1 cut(s) 1849
Bse3DI GCAATG 1 cut(s) 3209
BseBI CCWGG 8 cut(s) 777, 993, 1158, 1187, 1910, 2475, 2800, 3060
BseGI GGATG 8 cut(s) 40, 130, 445, 563, 988, 1332, 1465, 1933
BseLI CCNNNNNNNGG 5 cut(s) 1979, 2054, 2295, 2598, 2780
BseMI GCAATG 1 cut(s) 3209
BseNI ACTGG 3 cut(s) 39, 1938, 2019
BseRI GAGGAG 8 cut(s) 134, 491, 665, 2167, 2951, 3180, 3207, 3212
BseSI GKGCMC 2 cut(s) 988, 3124
BseYI CCCAGC 1 cut(s) 2294
BsgI GTGCAG 4 cut(s) 2015, 2084, 2921, 2973
Bsh1236I CGCG 3 cut(s) 53, 265, 2022
BshFI GGCC 7 cut(s) 50, 457, 1191, 2602, 2816, 2852, 3240
BshNI GGYRCC 1 cut(s) 3301
BshTI ACCGGT 2 cut(s) 2449, 2960
BsiHKAI GWGCWC 5 cut(s) 87, 318, 988, 2311, 3124
BsiHKCI CYCGRG 2 cut(s) 377, 1029
BsiSI CCGG 8 cut(s) 458, 569, 764, 2450, 2616, 2650, 2961, 3237
BsiWI CGTACG 1 cut(s) 966
BslFI GGGAC 5 cut(s) 394, 474, 1477, 2095, 2998
BslI CCNNNNNNNGG 5 cut(s) 1979, 2054, 2295, 2598, 2780
BsmAI GTCTC 6 cut(s) 495, 1119, 2057, 2395, 2624, 2798
BsmBI CGTCTC 2 cut(s) 495, 2798
BsmFI GGGAC 5 cut(s) 394, 474, 1477, 2095, 2998
BsmI GAATGC 2 cut(s) 715, 3289
BsnI GGCC 7 cut(s) 50, 457, 1191, 2602, 2816, 2852, 3240
Bso31I GGTCTC 2 cut(s) 2395, 2624
BsoBI CYCGRG 2 cut(s) 377, 1029
Bsp119I TTCGAA 1 cut(s) 408
Bsp1286I GDGCHC 7 cut(s) 87, 318, 988, 1276, 1390, 2311, 3124
Bsp1407I TGTACA 1 cut(s) 2664
Bsp1720I GCTNAGC 1 cut(s) 395
BspANI GGCC 7 cut(s) 50, 457, 1191, 2602, 2816, 2852, 3240
BspFNI CGCG 3 cut(s) 53, 265, 2022
BspHI TCATGA 1 cut(s) 3051
BspLI GGNNCC 6 cut(s) 1275, 1465, 2111, 2496, 2823, 3303
BspMAI CTGCAG 2 cut(s) 1723, 2383
BspMI ACCTGC 1 cut(s) 2944
BspOI GCTAGC 1 cut(s) 1803
BspPI GGATC 8 cut(s) 1570, 1631, 2039, 2268, 2276, 2616, 2816, 2829
BspQI GCTCTTC 1 cut(s) 884
BspT104I TTCGAA 1 cut(s) 408
BspT107I GGYRCC 1 cut(s) 3301
BspTNI GGTCTC 2 cut(s) 2395, 2624
BsrDI GCAATG 1 cut(s) 3209
BsrFI RCCGGY 2 cut(s) 2449, 2960
BsrGI TGTACA 1 cut(s) 2664
BsrI ACTGG 3 cut(s) 39, 1938, 2019
BssAI RCCGGY 2 cut(s) 2449, 2960
BssNI GRCGYC 3 cut(s) 997, 2807, 3111
BssT1I CCWWGG 1 cut(s) 2817
Bst2UI CCWGG 8 cut(s) 777, 993, 1158, 1187, 1910, 2475, 2800, 3060
Bst6I CTCTTC 7 cut(s) 39, 356, 380, 884, 945, 1076, 1536
BstACI GRCGYC 3 cut(s) 997, 2807, 3111
BstAPI GCANNNNNTGC 3 cut(s) 983, 1871, 2588
BstAUI TGTACA 1 cut(s) 2664
BstBAI YACGTR 1 cut(s) 2993
BstBI TTCGAA 1 cut(s) 408
BstC8I GCNNGC 6 cut(s) 106, 737, 1801, 2031, 2568, 2883
BstDSI CCRYGG 3 cut(s) 51, 449, 2810
BstEII GGTNACC 1 cut(s) 2956
BstF5I GGATG 8 cut(s) 40, 130, 445, 563, 988, 1332, 1465, 1933
BstFNI CGCG 3 cut(s) 53, 265, 2022
BstH2I RGCGCY 1 cut(s) 395
BstHHI GCGC 1 cut(s) 394
BstMAI GTCTC 6 cut(s) 495, 1119, 2057, 2395, 2624, 2798
BstNI CCWGG 8 cut(s) 777, 993, 1158, 1187, 1910, 2475, 2800, 3060
BstNSI RCATGY 1 cut(s) 2649
BstPI GGTNACC 1 cut(s) 2956
BstSFI CTRYAG 5 cut(s) 1391, 1428, 1719, 2379, 2409
BstSLI GKGCMC 2 cut(s) 988, 3124
BstUI CGCG 3 cut(s) 53, 265, 2022
BstV2I GAAGAC 3 cut(s) 249, 734, 3120
BstX2I RGATCY 5 cut(s) 113, 1636, 2273, 2329, 2821
BstYI RGATCY 5 cut(s) 113, 1636, 2273, 2329, 2821
Bsu36I CCTNAGG 1 cut(s) 1849
BsuI GTATCC 1 cut(s) 1897
BsuRI GGCC 7 cut(s) 50, 457, 1191, 2602, 2816, 2852, 3240
BtgI CCRYGG 3 cut(s) 51, 449, 2810
BtgZI GCGATG 1 cut(s) 2547
BtrI CACGTC 1 cut(s) 523
BtsCI GGATG 8 cut(s) 40, 130, 445, 563, 988, 1332, 1465, 1933
BtsI GCAGTG 2 cut(s) 789, 3141
BtsIMutI CAGTG 5 cut(s) 46, 789, 874, 3100, 3141
BveI ACCTGC 1 cut(s) 2944
Cac8I GCNNGC 6 cut(s) 106, 737, 1801, 2031, 2568, 2883
CciI TCATGA 1 cut(s) 3051
CfoI GCGC 1 cut(s) 394
Cfr10I RCCGGY 2 cut(s) 2449, 2960
Cfr42I CCGCGG 1 cut(s) 54
CseI GACGC 2 cut(s) 1005, 2815
Csp6I GTAC 9 cut(s) 141, 745, 967, 1476, 1488, 1841, 2018, 2347, 2665
CspAI ACCGGT 2 cut(s) 2449, 2960
CviAII CATG 5 cut(s) 421, 1880, 2646, 3004, 3052
CviQI GTAC 9 cut(s) 141, 745, 967, 1476, 1488, 1841, 2018, 2347, 2665
DraI TTTAAA 2 cut(s) 1503, 1534
DraIII CACNNNGTG 1 cut(s) 878
EaeI YGGCCR 1 cut(s) 455
Eam1104I CTCTTC 7 cut(s) 39, 356, 380, 884, 945, 1076, 1536
EarI CTCTTC 7 cut(s) 39, 356, 380, 884, 945, 1076, 1536
EciI GGCGGA 4 cut(s) 123, 218, 517, 2314
Ecl136II GAGCTC 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 2817
Eco24I GRGCYC 3 cut(s) 87, 1276, 1390
Eco31I GGTCTC 2 cut(s) 2395, 2624
Eco47I GGWCC 7 cut(s) 1046, 1111, 1424, 1464, 2109, 2494, 2762
Eco53kI GAGCTC 1 cut(s) 85
Eco57I CTGAAG 6 cut(s) 659, 2089, 2597, 2787, 2927, 2939
Eco72I CACGTG 1 cut(s) 2993
Eco81I CCTNAGG 1 cut(s) 1849
Eco88I CYCGRG 2 cut(s) 377, 1029
Eco91I GGTNACC 1 cut(s) 2956
EcoICRI GAGCTC 1 cut(s) 85
EcoO109I RGGNCCY 2 cut(s) 1424, 2109
EcoO65I GGTNACC 1 cut(s) 2956
EcoRI GAATTC 1 cut(s) 195
EcoRII CCWGG 8 cut(s) 775, 991, 1156, 1185, 1908, 2473, 2798, 3058
EcoT14I CCWWGG 1 cut(s) 2817
EcoT38I GRGCYC 3 cut(s) 87, 1276, 1390
ErhI CCWWGG 1 cut(s) 2817
Esp3I CGTCTC 2 cut(s) 495, 2798
FaeI CATG 5 cut(s) 424, 1883, 2649, 3007, 3055
FalI AAGNNNNNCTT 2 cut(s) 2316, 2348
FaqI GGGAC 5 cut(s) 394, 474, 1477, 2095, 2998
FatI CATG 5 cut(s) 420, 1879, 2645, 3003, 3051
FauI CCCGC 1 cut(s) 258
FauNDI CATATG 1 cut(s) 1567
FbaI TGATCA 3 cut(s) 883, 1309, 3043
FblI GTMKAC 2 cut(s) 2118, 3114
FokI GGATG 8 cut(s) 47, 137, 432, 570, 975, 1319, 1472, 1940
FriOI GRGCYC 3 cut(s) 87, 1276, 1390
GlaI GCGC 1 cut(s) 393
GsaI CCCAGC 1 cut(s) 2298
GsuI CTGGAG 2 cut(s) 1260, 2757
HaeII RGCGCY 1 cut(s) 395
HaeIII GGCC 7 cut(s) 50, 457, 1191, 2602, 2816, 2852, 3240
HapII CCGG 8 cut(s) 458, 569, 764, 2450, 2616, 2650, 2961, 3237
HgaI GACGC 2 cut(s) 1005, 2815
HhaI GCGC 1 cut(s) 394
Hin1I GRCGYC 3 cut(s) 997, 2807, 3111
Hin1II CATG 5 cut(s) 424, 1883, 2649, 3007, 3055
Hin6I GCGC 1 cut(s) 392
HinP1I GCGC 1 cut(s) 392
HincII GTYRAC 2 cut(s) 870, 3115
HindII GTYRAC 2 cut(s) 870, 3115
HpaII CCGG 8 cut(s) 458, 569, 764, 2450, 2616, 2650, 2961, 3237
Hpy166II GTNNAC 7 cut(s) 683, 870, 986, 2119, 3109, 3115, 3122
Hpy8I GTNNAC 7 cut(s) 683, 870, 986, 2119, 3109, 3115, 3122
Hpy99I CGWCG 3 cut(s) 2282, 3116, 3119
HpyCH4IV ACGT 9 cut(s) 488, 522, 965, 1474, 1624, 2305, 2992, 3111, 3350
HpySE526I ACGT 9 cut(s) 488, 522, 965, 1474, 1624, 2305, 2992, 3111, 3350
Hsp92I GRCGYC 3 cut(s) 997, 2807, 3111
Hsp92II CATG 5 cut(s) 424, 1883, 2649, 3007, 3055
HspAI GCGC 1 cut(s) 392
Ksp22I TGATCA 3 cut(s) 883, 1309, 3043
KspI CCGCGG 1 cut(s) 54
LguI GCTCTTC 1 cut(s) 884
LmnI GCTCC 7 cut(s) 82, 779, 1027, 1279, 2180, 2314, 2776
LweI GCATC 8 cut(s) 25, 828, 1345, 1780, 1812, 2510, 2665, 3045
MaeII ACGT 9 cut(s) 488, 522, 965, 1474, 1624, 2305, 2992, 3111, 3350
MfeI CAATTG 1 cut(s) 1647
MflI RGATCY 5 cut(s) 113, 1636, 2273, 2329, 2821
MhlI GDGCHC 7 cut(s) 87, 318, 988, 1276, 1390, 2311, 3124
MlyI GAGTC 2 cut(s) 2408, 2948
MmeI TCCRAC 4 cut(s) 1049, 1612, 2301, 3218
MroXI GAANNNNTTC 1 cut(s) 1575
MslI CAYNNNNRTG 2 cut(s) 309, 879
MspA1I CMGCKG 3 cut(s) 53, 1682, 2905
MspI CCGG 8 cut(s) 458, 569, 764, 2450, 2616, 2650, 2961, 3237
MunI CAATTG 1 cut(s) 1647
Mva1269I GAATGC 2 cut(s) 715, 3289
MvaI CCWGG 8 cut(s) 777, 993, 1158, 1187, 1910, 2475, 2800, 3060
MvnI CGCG 3 cut(s) 53, 265, 2022
NciI CCSGG 4 cut(s) 459, 569, 2651, 3237
NdeI CATATG 1 cut(s) 1567
NheI GCTAGC 1 cut(s) 1799
NlaIII CATG 5 cut(s) 424, 1883, 2649, 3007, 3055
NlaIV GGNNCC 6 cut(s) 1275, 1465, 2111, 2496, 2823, 3303
NmeAIII GCCGAG 2 cut(s) 592, 2834
NmuCI GTSAC 7 cut(s) 416, 518, 1930, 2139, 2463, 2789, 3320
NspI RCATGY 1 cut(s) 2649
NspV TTCGAA 1 cut(s) 408
OliI CACNNNNGTG 1 cut(s) 309
PaeR7I CTCGAG 1 cut(s) 1029
PagI TCATGA 1 cut(s) 3051
PasI CCCWGGG 1 cut(s) 2799
PciSI GCTCTTC 1 cut(s) 884
PcsI WCGNNNNNNNCGW 3 cut(s) 252, 561, 1003
PctI GAATGC 2 cut(s) 715, 3289
PdmI GAANNNNTTC 1 cut(s) 1575
PfeI GAWTC 9 cut(s) 89, 179, 284, 622, 670, 796, 1955, 2438, 2914
Pfl23II CGTACG 1 cut(s) 966
PfoI TCCNGGA 1 cut(s) 991
PinAI ACCGGT 2 cut(s) 2449, 2960
PleI GAGTC 2 cut(s) 2408, 2947
PmaCI CACGTG 1 cut(s) 2993
PmlI CACGTG 1 cut(s) 2993
PpsI GAGTC 2 cut(s) 2408, 2947
Ppu21I YACGTR 1 cut(s) 2993
PpuMI RGGWCCY 2 cut(s) 1424, 2109
Psp124BI GAGCTC 1 cut(s) 87
Psp5II RGGWCCY 2 cut(s) 1424, 2109
Psp6I CCWGG 8 cut(s) 775, 991, 1156, 1185, 1908, 2473, 2798, 3058
PspCI CACGTG 1 cut(s) 2993
PspEI GGTNACC 1 cut(s) 2956
PspFI CCCAGC 1 cut(s) 2294
PspGI CCWGG 8 cut(s) 775, 991, 1156, 1185, 1908, 2473, 2798, 3058
PspLI CGTACG 1 cut(s) 966
PspN4I GGNNCC 6 cut(s) 1275, 1465, 2111, 2496, 2823, 3303
PspPPI RGGWCCY 2 cut(s) 1424, 2109
PspXI VCTCGAGB 1 cut(s) 1029
PstI CTGCAG 2 cut(s) 1723, 2383
PsuI RGATCY 5 cut(s) 113, 1636, 2273, 2329, 2821
PvuII CAGCTG 2 cut(s) 1682, 2905
RsaI GTAC 9 cut(s) 142, 746, 968, 1477, 1489, 1842, 2019, 2348, 2666
RsaNI GTAC 9 cut(s) 141, 745, 967, 1476, 1488, 1841, 2018, 2347, 2665
RseI CAYNNNNRTG 2 cut(s) 309, 879
SacI GAGCTC 1 cut(s) 87
SacII CCGCGG 1 cut(s) 54
SalI GTCGAC 1 cut(s) 3113
SapI GCTCTTC 1 cut(s) 884
SchI GAGTC 2 cut(s) 2408, 2948
SduI GDGCHC 7 cut(s) 87, 318, 988, 1276, 1390, 2311, 3124
SfaNI GCATC 8 cut(s) 25, 828, 1345, 1780, 1812, 2510, 2665, 3045
SfcI CTRYAG 5 cut(s) 1391, 1428, 1719, 2379, 2409
Sfr274I CTCGAG 1 cut(s) 1029
Sfr303I CCGCGG 1 cut(s) 54
SfuI TTCGAA 1 cut(s) 408
SgrBI CCGCGG 1 cut(s) 54
SgrDI CGTCGACG 1 cut(s) 3113
SinI GGWCC 7 cut(s) 1046, 1111, 1424, 1464, 2109, 2494, 2762
SlaI CTCGAG 1 cut(s) 1029
SmiMI CAYNNNNRTG 2 cut(s) 309, 879
SmlI CTYRAG 2 cut(s) 1029, 2468
SmoI CTYRAG 2 cut(s) 1029, 2468
SpeI ACTAGT 2 cut(s) 3023, 3317
SstI GAGCTC 1 cut(s) 87
StyI CCWWGG 1 cut(s) 2817
TaiI ACGT 9 cut(s) 491, 525, 968, 1477, 1627, 2308, 2995, 3114, 3353
TatI WGTACW 1 cut(s) 2664
TauI GCSGC 3 cut(s) 53, 502, 1865
TfiI GAWTC 9 cut(s) 89, 179, 284, 622, 670, 796, 1955, 2438, 2914
TscAI CASTG 5 cut(s) 46, 789, 881, 3100, 3148
TseFI GTSAC 7 cut(s) 416, 518, 1930, 2139, 2463, 2789, 3320
Tsp45I GTSAC 7 cut(s) 416, 518, 1930, 2139, 2463, 2789, 3320
TspGWI ACGGA 8 cut(s) 158, 438, 523, 570, 1729, 2507, 2612, 3164
TspRI CASTG 5 cut(s) 46, 789, 881, 3100, 3148
VneI GTGCAC 2 cut(s) 984, 3120
VpaK11BI GGWCC 7 cut(s) 1046, 1111, 1424, 1464, 2109, 2494, 2762
XapI RAATTY 4 cut(s) 195, 1574, 2745, 2999
XceI RCATGY 1 cut(s) 2649
XhoI CTCGAG 1 cut(s) 1029
XmiI GTMKAC 2 cut(s) 2118, 3114
XmnI GAANNNNTTC 1 cut(s) 1575
ZraI GACGTC 1 cut(s) 3112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.