pycom04g19260

Nucleolar protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011411
Physical Location & Seq
Reverse (-)
21037002 .. 21039309
2308 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g19260.1

Sequence Viewer

Length: 1329 bp
ATGGAGTTGATGAGAGGTGTAAGAGCTCAGTTGACTGAGCTCATAGCTGGTCTACAAGTTCAAGATCTGGCACCAATGAGCTTGGGTTTATCTCACAGCTTATCTCGATTCAAGCTCAAGTTCAGTGCTGATAAGGTTGATACAATGGTTATTCAAGCCATTGGTTTGCTTGATGATCTTGATAAAGAGCTAAATACATATGCAATGAGGGTTCGAGAATGGTATGGTTGGCATTTTCCAGAGCTTGCTAAGATTGTTCAGGACAACATCCTTTATGCGAAGGCAGTTAAGCTGATGGGCTATCGTTCTAATGCTGCAAAGCTTGACTTCTCTGAGATATTGCCAGAGGAGGTTGAAACTGAATTGAAGGATGCAGCAATGATATCCATGGGAACAGAAGTTAGCGAGCTTGATTTGATGAACATCAAAGAACTCTGTGACCAAGTCCTTTCTCTCTCTGAGTACAGAGCTCAGCTATATGACTATTTGAAAAATAGGATGAACACCATTGCACCAAATTTGACTGCTCTTGTTGGAGAGCTCGTCGGTGCTCGCCTCATTTCCCATGGTGGTAGCTTGTTGAACCTTGCAAAGCAGCCTGGAAGCACAGTTCAGATTCTTGGAGCCGAAAAGGCCCTCTTCAGAGCACTGAAGACCAAGCACGCAACACCAAAATATGGGCTTATCTACCATGCATCCTTGATTGGTAAAGCAGCGCCAAAGCTGAAGGGTAAAATTTCTCGGTCACTTGCTAGTAAAGCAGCATTGGCAATTCGAGTTGATGCTCTTGGAGACGGTCAAGATAACTCAATGGGAGTGGAAAATTATCAAAAGCTTGAAGCACGGTTAAGGAATCTTGAAGGCAGAGAATTAGGTCACCATGCTGGGTCAGCTAAAGGCAAACCAAAGATTGAAGTCTATGACAAGGATCGCAAGAAGGGAGCCGCAGGATTGATAACTGCTGCCAAGGCATATAATCCTGCAGCAGATTCGGTTCTTGGGAAAATGACGCCTTCTAAGACCGAGAAACCAGTTGCTGAGGTTCCTGTTACTGAAGATAAGAAAGAGAAGAAGAAAAAGAAGAAGAAGGCTGATGACGAGGACATGCCTGACGGAAATGGCAATGTGGAACCAGAAGTTGAGGAGCCTGCAAAGAAGGAAAAGAAGAAGAAAAAGAAGCATTCAGCTGAGGATGCTGAAGTAGTTGCTGCAGTTGAAAATGAAGATGCAGGAGAGAAGAAGAGGAAAAGAAAGCATGCTGAAGAAGAAGAAACGGAGGTGCCAAGCGAGAAGAAAGAGAAAAAGAAGAAGAAGAAGAGCAAGGATTGA

Protein Analysis

443

Amino Acids

48.87

Weight (kDa)

9.16

Isoelectric Point (pI)

33.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop PF01798 56 - 284 3.8e-86 snoRNA binding domain, fibrillarin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011411)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05060 AT5G27120
fragaria_vesca FvH4_6g04090 FvH4_6g04090
malus_domestica MD04G1216600.v1.1 MD12G1233200.v1.1
prunus_persica Prupe.6G336300_v2.0.a1
pyrus_communis pycom04g19260 pycom12g21570
rosa_chinensis RchiOBHm_Chr3g0452641
rosa_laevigata RLG00000025576
rosa_multiflora Rmu_sc0022184.1_g000001
rosa_roxburghii Rroxscaffold_6G00429680
rosa_rugosa Rorug02G0642100 Rorug02G0642100
rosa_samantha Rh3AG044500 Rh3BG046800 Rh3CG045300 Rh3DG045700
rosa_wichuraiana Rw3G003470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 70, 1279
AccB7I CCANNNNNTGG 1 cut(s) 677
AccI GTMKAC 1 cut(s) 52
AciI CCGC 1 cut(s) 945
AclWI GGATC 1 cut(s) 936
AcsI RAATTY 2 cut(s) 517, 735
AcuI CTGAAG 6 cut(s) 625, 671, 746, 1074, 1218, 1281
AcyI GRCGYC 1 cut(s) 1010
AfaI GTAC 1 cut(s) 464
AfiI CCNNNNNNNGG 1 cut(s) 677
AjnI CCWGG 1 cut(s) 598
AloI GAACNNNNNNTCC 2 cut(s) 594, 626
Alw21I GWGCWC 6 cut(s) 28, 42, 472, 543, 553, 649
Alw26I GTCTC 1 cut(s) 786
AlwI GGATC 1 cut(s) 936
AlwNI CAGNNNCTG 1 cut(s) 1037
AoxI GGCC 1 cut(s) 633
ApeKI GCWGC 8 cut(s) 314, 374, 595, 713, 761, 962, 983, 1208
ApoI RAATTY 2 cut(s) 517, 735
AspLEI GCGC 1 cut(s) 718
AspS9I GGNCC 1 cut(s) 634
AsuHPI GGTGA 1 cut(s) 869
BaeI ACNNNNGTAYC 2 cut(s) 132, 165
BanI GGYRCC 2 cut(s) 70, 1279
BanII GRGCYC 4 cut(s) 28, 42, 472, 543
BbsI GAAGAC 1 cut(s) 659
Bbv12I GWGCWC 6 cut(s) 28, 42, 472, 543, 553, 649
BbvCI CCTCAGC 2 cut(s) 1038, 1188
BbvI GCAGC 8 cut(s) 301, 386, 607, 725, 773, 949, 995, 1195
BccI CCATC 1 cut(s) 289
BcgI CGANNNNNNTGC 2 cut(s) 972, 1006
BciT130I CCWGG 1 cut(s) 600
BcoDI GTCTC 1 cut(s) 786
BfaI CTAG 1 cut(s) 753
BfmI CTRYAG 2 cut(s) 981, 1209
BfoI RGCGCY 1 cut(s) 719
BglI GCCNNNNNGGC 1 cut(s) 632
BglII AGATCT 1 cut(s) 64
BisI GCNGC 9 cut(s) 315, 375, 596, 714, 762, 945, 963, 984, 1209
BlpI GCTNAGC 1 cut(s) 471
BlsI GCNGC 9 cut(s) 316, 376, 597, 715, 763, 946, 964, 985, 1210
Bme1390I CCNGG 1 cut(s) 600
BmgT120I GGNCC 1 cut(s) 634
BmiI GGNNCC 7 cut(s) 72, 625, 943, 1044, 1131, 1146, 1281
BmrFI CCNGG 1 cut(s) 600
BmsI GCATC 5 cut(s) 361, 704, 772, 1183, 1216
BpiI GAAGAC 1 cut(s) 659
Bpu10I CCTNAGC 2 cut(s) 1038, 1188
Bpu1102I GCTNAGC 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 101
BsaBI GATNNNNATC 1 cut(s) 422
BsaHI GRCGYC 1 cut(s) 1010
BsaJI CCNNGG 3 cut(s) 387, 565, 966
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bsc4I CCNNNNNNNGG 1 cut(s) 677
Bse1I ACTGG 1 cut(s) 1031
Bse3DI GCAATG 4 cut(s) 210, 384, 507, 1129
Bse8I GATNNNNATC 1 cut(s) 422
BseBI CCWGG 1 cut(s) 600
BseDI CCNNGG 3 cut(s) 387, 565, 966
BseGI GGATG 5 cut(s) 267, 376, 504, 695, 1198
BseJI GATNNNNATC 1 cut(s) 422
BseLI CCNNNNNNNGG 1 cut(s) 677
BseMI GCAATG 4 cut(s) 210, 384, 507, 1129
BseMII CTCAG 7 cut(s) 27, 41, 324, 450, 485, 1029, 1179
BseNI ACTGG 1 cut(s) 1031
BseRI GAGGAG 2 cut(s) 362, 1157
BseXI GCAGC 8 cut(s) 301, 386, 607, 725, 773, 949, 995, 1195
BseYI CCCAGC 1 cut(s) 884
BshFI GGCC 1 cut(s) 635
BshNI GGYRCC 2 cut(s) 70, 1279
BsiHKAI GWGCWC 6 cut(s) 28, 42, 472, 543, 553, 649
BslI CCNNNNNNNGG 1 cut(s) 677
BsmAI GTCTC 1 cut(s) 786
BsmBI CGTCTC 1 cut(s) 786
BsmI GAATGC 1 cut(s) 1180
BsnI GGCC 1 cut(s) 635
Bsp1286I GDGCHC 6 cut(s) 28, 42, 472, 543, 553, 649
Bsp143I GATC 3 cut(s) 64, 175, 928
Bsp1720I GCTNAGC 1 cut(s) 471
Bsp19I CCATGG 2 cut(s) 387, 565
BspACI CCGC 1 cut(s) 945
BspANI GGCC 1 cut(s) 635
BspCNI CTCAG 7 cut(s) 28, 40, 325, 451, 484, 1030, 1180
BspLI GGNNCC 7 cut(s) 72, 625, 943, 1044, 1131, 1146, 1281
BspMAI CTGCAG 2 cut(s) 985, 1213
BspPI GGATC 1 cut(s) 936
BspQI GCTCTTC 1 cut(s) 1310
BspT107I GGYRCC 2 cut(s) 70, 1279
BsrDI GCAATG 4 cut(s) 210, 384, 507, 1129
BsrI ACTGG 1 cut(s) 1031
BssECI CCNNGG 3 cut(s) 387, 565, 966
BssMI GATC 3 cut(s) 64, 175, 928
BssNI GRCGYC 1 cut(s) 1010
BssT1I CCWWGG 3 cut(s) 387, 565, 966
Bst2UI CCWGG 1 cut(s) 600
Bst4CI ACNGT 3 cut(s) 610, 797, 846
Bst6I CTCTTC 3 cut(s) 644, 1235, 1310
BstACI GRCGYC 1 cut(s) 1010
BstC8I GCNNGC 6 cut(s) 246, 407, 553, 663, 1149, 1257
BstDEI CTNAG 9 cut(s) 27, 36, 249, 333, 459, 471, 1017, 1038, 1188
BstDSI CCRYGG 2 cut(s) 387, 565
BstEII GGTNACC 1 cut(s) 875
BstF5I GGATG 5 cut(s) 267, 376, 504, 695, 1198
BstH2I RGCGCY 1 cut(s) 719
BstHHI GCGC 1 cut(s) 718
BstKTI GATC 3 cut(s) 67, 178, 931
BstMAI GTCTC 1 cut(s) 786
BstMBI GATC 3 cut(s) 64, 175, 928
BstMWI GCNNNNNNNGC 6 cut(s) 632, 758, 767, 890, 968, 1193
BstNI CCWGG 1 cut(s) 600
BstNSI RCATGY 2 cut(s) 1108, 1259
BstPI GGTNACC 1 cut(s) 875
BstSCI CCNGG 1 cut(s) 598
BstSFI CTRYAG 2 cut(s) 981, 1209
BstV1I GCAGC 8 cut(s) 301, 386, 607, 725, 773, 949, 995, 1195
BstV2I GAAGAC 1 cut(s) 659
BstX2I RGATCY 1 cut(s) 64
BstYI RGATCY 1 cut(s) 64
BsuRI GGCC 1 cut(s) 635
BtgI CCRYGG 2 cut(s) 387, 565
BtsCI GGATG 5 cut(s) 267, 376, 504, 695, 1198
BtsIMutI CAGTG 2 cut(s) 130, 647
Cac8I GCNNGC 6 cut(s) 246, 407, 553, 663, 1149, 1257
CaiI CAGNNNCTG 1 cut(s) 1037
CfoI GCGC 1 cut(s) 718
Cfr13I GGNCC 1 cut(s) 634
CseI GACGC 1 cut(s) 1018
Csp6I GTAC 1 cut(s) 463
CspCI CAANNNNNGTGG 2 cut(s) 798, 833
CviAII CATG 6 cut(s) 388, 566, 692, 881, 1105, 1256
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 9 cut(s) 27, 36, 249, 333, 459, 471, 1017, 1038, 1188
DpnI GATC 3 cut(s) 66, 177, 930
DpnII GATC 3 cut(s) 64, 175, 928
Eam1104I CTCTTC 3 cut(s) 644, 1235, 1310
EarI CTCTTC 3 cut(s) 644, 1235, 1310
Ecl136II GAGCTC 4 cut(s) 26, 40, 470, 541
Eco130I CCWWGG 3 cut(s) 387, 565, 966
Eco24I GRGCYC 4 cut(s) 28, 42, 472, 543
Eco32I GATATC 1 cut(s) 384
Eco53kI GAGCTC 4 cut(s) 26, 40, 470, 541
Eco57I CTGAAG 6 cut(s) 625, 671, 746, 1074, 1218, 1281
Eco91I GGTNACC 1 cut(s) 875
EcoICRI GAGCTC 4 cut(s) 26, 40, 470, 541
EcoO109I RGGNCCY 1 cut(s) 634
EcoO65I GGTNACC 1 cut(s) 875
EcoRII CCWGG 1 cut(s) 598
EcoRV GATATC 1 cut(s) 384
EcoT14I CCWWGG 3 cut(s) 387, 565, 966
EcoT22I ATGCAT 1 cut(s) 697
EcoT38I GRGCYC 4 cut(s) 28, 42, 472, 543
ErhI CCWWGG 3 cut(s) 387, 565, 966
Esp3I CGTCTC 1 cut(s) 786
FaeI CATG 6 cut(s) 391, 569, 695, 884, 1108, 1259
FalI AAGNNNNNCTT 4 cut(s) 311, 343, 623, 655
FatI CATG 6 cut(s) 387, 565, 691, 880, 1104, 1255
FauNDI CATATG 1 cut(s) 199
FblI GTMKAC 1 cut(s) 52
Fnu4HI GCNGC 9 cut(s) 315, 375, 596, 714, 762, 945, 963, 984, 1209
FokI GGATG 5 cut(s) 254, 383, 511, 682, 1205
FriOI GRGCYC 4 cut(s) 28, 42, 472, 543
Fsp4HI GCNGC 9 cut(s) 315, 375, 596, 714, 762, 945, 963, 984, 1209
FspBI CTAG 1 cut(s) 753
GlaI GCGC 1 cut(s) 717
GluI GCNGC 9 cut(s) 315, 375, 596, 714, 762, 945, 963, 984, 1209
GsaI CCCAGC 1 cut(s) 888
HaeII RGCGCY 1 cut(s) 719
HaeIII GGCC 1 cut(s) 635
HgaI GACGC 1 cut(s) 1018
HhaI GCGC 1 cut(s) 718
Hin1I GRCGYC 1 cut(s) 1010
Hin1II CATG 6 cut(s) 391, 569, 695, 884, 1108, 1259
Hin6I GCGC 1 cut(s) 716
HinP1I GCGC 1 cut(s) 716
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HindIII AAGCTT 2 cut(s) 320, 833
HinfI GANTC 4 cut(s) 108, 616, 853, 989
HphI GGTGA 1 cut(s) 869
Hpy166II GTNNAC 2 cut(s) 33, 53
Hpy188I TCNGA 4 cut(s) 334, 460, 615, 644
Hpy188III TCNNGA 8 cut(s) 62, 105, 179, 215, 239, 260, 800, 857
Hpy8I GTNNAC 2 cut(s) 33, 53
Hpy99I CGWCG 1 cut(s) 548
HpyAV CCTTC 8 cut(s) 274, 361, 721, 854, 931, 1023, 1081, 1150
HpyCH4III ACNGT 3 cut(s) 610, 797, 846
HpyF10VI GCNNNNNNNGC 6 cut(s) 632, 758, 767, 890, 968, 1193
HpyF3I CTNAG 9 cut(s) 27, 36, 249, 333, 459, 471, 1017, 1038, 1188
Hsp92I GRCGYC 1 cut(s) 1010
Hsp92II CATG 6 cut(s) 391, 569, 695, 884, 1108, 1259
HspAI GCGC 1 cut(s) 716
Kzo9I GATC 3 cut(s) 64, 175, 928
LguI GCTCTTC 1 cut(s) 1310
LmnI GCTCC 3 cut(s) 623, 941, 1144
Lsp1109I GCAGC 8 cut(s) 301, 386, 607, 725, 773, 949, 995, 1195
LweI GCATC 5 cut(s) 361, 704, 772, 1183, 1216
MaeI CTAG 1 cut(s) 753
MaeIII GTNAC 4 cut(s) 437, 744, 875, 1048
MalI GATC 3 cut(s) 66, 177, 930
MboI GATC 3 cut(s) 64, 175, 928
MflI RGATCY 1 cut(s) 64
MhlI GDGCHC 6 cut(s) 28, 42, 472, 543, 553, 649
MluCI AATT 6 cut(s) 362, 517, 735, 771, 823, 869
MmeI TCCRAC 1 cut(s) 514
Mph1103I ATGCAT 1 cut(s) 697
MseI TTAA 2 cut(s) 288, 848
MspA1I CMGCKG 1 cut(s) 1187
MspR9I CCNGG 1 cut(s) 600
Mva1269I GAATGC 1 cut(s) 1180
MvaI CCWGG 1 cut(s) 600
MwoI GCNNNNNNNGC 6 cut(s) 632, 758, 767, 890, 968, 1193
NcoI CCATGG 2 cut(s) 387, 565
NdeI CATATG 1 cut(s) 199
NdeII GATC 3 cut(s) 64, 175, 928
NlaIII CATG 6 cut(s) 391, 569, 695, 884, 1108, 1259
NlaIV GGNNCC 7 cut(s) 72, 625, 943, 1044, 1131, 1146, 1281
NmuCI GTSAC 3 cut(s) 437, 744, 875
NsiI ATGCAT 1 cut(s) 697
NspI RCATGY 2 cut(s) 1108, 1259
PaeI GCATGC 1 cut(s) 1259
PciSI GCTCTTC 1 cut(s) 1310
PctI GAATGC 1 cut(s) 1180
PfeI GAWTC 4 cut(s) 108, 616, 853, 989
PflFI GACNNNGTC 1 cut(s) 443
PflMI CCANNNNNTGG 1 cut(s) 677
PkrI GCNGC 9 cut(s) 316, 376, 597, 715, 763, 946, 964, 985, 1210
Psp124BI GAGCTC 4 cut(s) 28, 42, 472, 543
Psp6I CCWGG 1 cut(s) 598
PspEI GGTNACC 1 cut(s) 875
PspFI CCCAGC 1 cut(s) 884
PspGI CCWGG 1 cut(s) 598
PspN4I GGNNCC 7 cut(s) 72, 625, 943, 1044, 1131, 1146, 1281
PspPI GGNCC 1 cut(s) 634
PstI CTGCAG 2 cut(s) 985, 1213
PstNI CAGNNNCTG 1 cut(s) 1037
PsuI RGATCY 1 cut(s) 64
PsyI GACNNNGTC 1 cut(s) 443
PvuII CAGCTG 1 cut(s) 1187
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
SacI GAGCTC 4 cut(s) 28, 42, 472, 543
SapI GCTCTTC 1 cut(s) 1310
SaqAI TTAA 2 cut(s) 288, 848
SatI GCNGC 9 cut(s) 315, 375, 596, 714, 762, 945, 963, 984, 1209
Sau3AI GATC 3 cut(s) 64, 175, 928
Sau96I GGNCC 1 cut(s) 634
ScrFI CCNGG 1 cut(s) 600
SduI GDGCHC 6 cut(s) 28, 42, 472, 543, 553, 649
SfaNI GCATC 5 cut(s) 361, 704, 772, 1183, 1216
SfcI CTRYAG 2 cut(s) 981, 1209
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
SphI GCATGC 1 cut(s) 1259
Sse9I AATT 6 cut(s) 362, 517, 735, 771, 823, 869
SsiI CCGC 1 cut(s) 945
SspMI CTAG 1 cut(s) 753
SstI GAGCTC 4 cut(s) 28, 42, 472, 543
StyD4I CCNGG 1 cut(s) 598
StyI CCWWGG 3 cut(s) 387, 565, 966
TaaI ACNGT 3 cut(s) 610, 797, 846
TaqI TCGA 3 cut(s) 106, 214, 775
TaqII GACCGA 2 cut(s) 732, 1037
TasI AATT 6 cut(s) 362, 517, 735, 771, 823, 869
TatI WGTACW 1 cut(s) 462
TauI GCSGC 1 cut(s) 947
TfiI GAWTC 4 cut(s) 108, 616, 853, 989
Tru1I TTAA 2 cut(s) 288, 848
Tru9I TTAA 2 cut(s) 288, 848
TscAI CASTG 2 cut(s) 130, 654
TseFI GTSAC 3 cut(s) 437, 744, 875
TseI GCWGC 8 cut(s) 314, 374, 595, 713, 761, 962, 983, 1208
Tsp45I GTSAC 3 cut(s) 437, 744, 875
TspDTI ATGAA 3 cut(s) 434, 515, 1236
TspGWI ACGGA 2 cut(s) 1128, 1289
TspRI CASTG 2 cut(s) 130, 654
Tth111I GACNNNGTC 1 cut(s) 443
Van91I CCANNNNNTGG 1 cut(s) 677
XapI RAATTY 2 cut(s) 517, 735
XceI RCATGY 2 cut(s) 1108, 1259
XmiI GTMKAC 1 cut(s) 52
XspI CTAG 1 cut(s) 753
Zsp2I ATGCAT 1 cut(s) 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.