pycom05g01680

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0019801
Physical Location & Seq
Reverse (-)
2099648 .. 2100271
624 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g01680.1

Sequence Viewer

Length: 624 bp
ATGACTGCAACTACAGGCTCAGACTGCGAGAATCCAGGCTCCTTAGGCATGTCTGCCACTAAAAGCTCTCAGCCAGCTTGCGCCCAAATCTGTTGGGTCGTCTTTGTGATTGCTCTTTTCCTCATGCCTTTTCCAGTTATAATTGGGCTCCAGTTGCAGTCCAGCATAAGAATTTCGGAGTACAAATGCCCCGACTTCACCATCAACGGTGCTTATCTAAGCCAATTCAATTACACCCAAAGCAATCGTACTCTCTTGTACAACCTCGCCCTCAACATCACCTTCACAAACCCTAAGAAGAAACTCTTCCACACGTTGATTGACGTCAAACTCAGTGCATACTATCAAGACAAGAGAATTGGTCAGGTGACTTTGATGGATCTGTGGACGTCGACACACAAGAACACAGCTGTTTTTCAGAATGTAGCCGTTCAAGAGCACGACATATTGCTTCAGGAATATGCGACCCCGGCAGCTGATCTTTACAGTATCAACGTGGTCATTGCTTTCCAGGATGAGAACAATCAACGGGGTGAGGTCATCTACAATCTAAGGCTTCCTTTGAGTTTCAACGGGACATCTTGGGATGCTGACAAGACTGCAAACTGCCCCATACCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

208

Amino Acids

23.16

Weight (kDa)

5.87

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019801)

Species Orthologous Gene IDs
prunus_persica Prupe.1G185400_v2.0.a1 Prupe.1G413200_v2.0.a1 Prupe.6G103200_v2.0.a1
pyrus_communis pycom05g01680 pycom14g04070 pycom14g04080 pycom14g04090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 140
AatII GACGTC 2 cut(s) 327, 392
AccI GTMKAC 1 cut(s) 392
AclWI GGATC 1 cut(s) 387
AcsI RAATTY 1 cut(s) 171
AcuI CTGAAG 1 cut(s) 437
AcyI GRCGYC 2 cut(s) 324, 389
AfaI GTAC 3 cut(s) 182, 250, 260
AflIII ACRYGT 1 cut(s) 312
AgsI TTSAA 3 cut(s) 229, 434, 571
AjnI CCWGG 2 cut(s) 34, 510
AluBI AGCT 4 cut(s) 66, 77, 410, 476
AluI AGCT 4 cut(s) 66, 77, 410, 476
Alw21I GWGCWC 1 cut(s) 441
AlwI GGATC 1 cut(s) 387
ApeKI GCWGC 1 cut(s) 473
ApoI RAATTY 1 cut(s) 171
Asp700I GAANNNNTTC 1 cut(s) 305
AspLEI GCGC 1 cut(s) 83
AsuC2I CCSGG 1 cut(s) 470
AsuHPI GGTGA 4 cut(s) 190, 271, 379, 545
AxyI CCTNAGG 1 cut(s) 43
BanII GRGCYC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 485
BccI CCATC 2 cut(s) 209, 370
BceAI ACGGC 1 cut(s) 413
BciT130I CCWGG 2 cut(s) 36, 512
BcnI CCSGG 1 cut(s) 470
BfmI CTRYAG 1 cut(s) 12
BisI GCNGC 1 cut(s) 474
BlsI GCNGC 1 cut(s) 475
Bme1390I CCNGG 3 cut(s) 36, 470, 512
BmiI GGNNCC 2 cut(s) 40, 149
BmrFI CCNGG 3 cut(s) 36, 470, 512
BmsI GCATC 1 cut(s) 577
BpmI CTGGAG 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 470
BsaHI GRCGYC 2 cut(s) 324, 389
BsaJI CCNNGG 1 cut(s) 468
Bse1I ACTGG 2 cut(s) 134, 151
Bse21I CCTNAGG 1 cut(s) 43
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 2 cut(s) 36, 512
BseDI CCNNGG 1 cut(s) 468
BseGI GGATG 2 cut(s) 520, 592
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 3 cut(s) 33, 83, 346
BseNI ACTGG 2 cut(s) 134, 151
BseXI GCAGC 1 cut(s) 485
BsiHKAI GWGCWC 1 cut(s) 441
BsiSI CCGG 1 cut(s) 470
BslFI GGGAC 1 cut(s) 589
BsmFI GGGAC 1 cut(s) 589
Bsp1286I GDGCHC 2 cut(s) 150, 441
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 2 cut(s) 379, 478
BspCNI CTCAG 3 cut(s) 32, 82, 345
BspLI GGNNCC 2 cut(s) 40, 149
BspPI GGATC 1 cut(s) 387
BsrDI GCAATG 1 cut(s) 501
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 2 cut(s) 134, 151
BssECI CCNNGG 1 cut(s) 468
BssMI GATC 2 cut(s) 379, 478
BssNI GRCGYC 2 cut(s) 324, 389
Bst2UI CCWGG 2 cut(s) 36, 512
Bst4CI ACNGT 2 cut(s) 209, 488
Bst6I CTCTTC 1 cut(s) 311
BstACI GRCGYC 2 cut(s) 324, 389
BstAUI TGTACA 1 cut(s) 258
BstC8I GCNNGC 2 cut(s) 75, 79
BstDEI CTNAG 7 cut(s) 19, 43, 69, 218, 294, 332, 551
BstF5I GGATG 2 cut(s) 520, 592
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 2 cut(s) 382, 481
BstMBI GATC 2 cut(s) 379, 478
BstMWI GCNNNNNNNGC 4 cut(s) 24, 45, 154, 470
BstNI CCWGG 2 cut(s) 36, 512
BstNSI RCATGY 1 cut(s) 52
BstSCI CCNGG 3 cut(s) 34, 468, 510
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 1 cut(s) 485
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
Bsu36I CCTNAGG 1 cut(s) 43
BtsCI GGATG 2 cut(s) 520, 592
BtsIMutI CAGTG 1 cut(s) 340
Cac8I GCNNGC 2 cut(s) 75, 79
CfoI GCGC 1 cut(s) 83
Csp6I GTAC 3 cut(s) 181, 249, 259
CviAII CATG 2 cut(s) 49, 124
CviQI GTAC 3 cut(s) 181, 249, 259
DdeI CTNAG 7 cut(s) 19, 43, 69, 218, 294, 332, 551
DpnI GATC 2 cut(s) 381, 480
DpnII GATC 2 cut(s) 379, 478
Eam1104I CTCTTC 1 cut(s) 311
EarI CTCTTC 1 cut(s) 311
Eco24I GRGCYC 1 cut(s) 150
Eco57I CTGAAG 1 cut(s) 437
Eco81I CCTNAGG 1 cut(s) 43
EcoRII CCWGG 2 cut(s) 34, 510
EcoT38I GRGCYC 1 cut(s) 150
FaeI CATG 2 cut(s) 52, 127
FaiI YATR 9 cut(s) 50, 125, 140, 167, 340, 446, 462, 614, 622
FalI AAGNNNNNCTT 4 cut(s) 290, 322, 544, 576
FaqI GGGAC 1 cut(s) 589
FatI CATG 2 cut(s) 48, 123
FblI GTMKAC 1 cut(s) 392
Fnu4HI GCNGC 1 cut(s) 474
FokI GGATG 2 cut(s) 527, 599
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 1 cut(s) 474
GlaI GCGC 1 cut(s) 82
GluI GCNGC 1 cut(s) 474
GsuI CTGGAG 1 cut(s) 134
HapII CCGG 1 cut(s) 470
HhaI GCGC 1 cut(s) 83
Hin1I GRCGYC 2 cut(s) 324, 389
Hin1II CATG 2 cut(s) 52, 127
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HincII GTYRAC 1 cut(s) 393
HindII GTYRAC 1 cut(s) 393
HinfI GANTC 1 cut(s) 31
HpaII CCGG 1 cut(s) 470
HphI GGTGA 4 cut(s) 190, 271, 379, 545
Hpy166II GTNNAC 2 cut(s) 387, 393
Hpy188I TCNGA 3 cut(s) 22, 178, 420
Hpy188III TCNNGA 3 cut(s) 347, 434, 455
Hpy8I GTNNAC 2 cut(s) 387, 393
Hpy99I CGWCG 1 cut(s) 394
HpyAV CCTTC 1 cut(s) 292
HpyCH4III ACNGT 2 cut(s) 209, 488
HpyCH4IV ACGT 4 cut(s) 314, 324, 389, 495
HpyCH4V TGCA 4 cut(s) 8, 157, 338, 602
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 45, 154, 470
HpyF3I CTNAG 7 cut(s) 19, 43, 69, 218, 294, 332, 551
HpySE526I ACGT 4 cut(s) 314, 324, 389, 495
Hsp92I GRCGYC 2 cut(s) 324, 389
Hsp92II CATG 2 cut(s) 52, 127
HspAI GCGC 1 cut(s) 81
Kzo9I GATC 2 cut(s) 379, 478
LmnI GCTCC 2 cut(s) 44, 153
Lsp1109I GCAGC 1 cut(s) 485
LweI GCATC 1 cut(s) 577
MaeII ACGT 4 cut(s) 314, 324, 389, 495
MaeIII GTNAC 1 cut(s) 367
MalI GATC 2 cut(s) 381, 480
MboI GATC 2 cut(s) 379, 478
MboII GAAGA 2 cut(s) 298, 310
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 2 cut(s) 150, 441
MluCI AATT 5 cut(s) 141, 171, 224, 229, 357
MnlI CCTC 4 cut(s) 131, 275, 281, 529
MroXI GAANNNNTTC 1 cut(s) 305
MspA1I CMGCKG 2 cut(s) 410, 476
MspI CCGG 1 cut(s) 470
MspR9I CCNGG 3 cut(s) 36, 470, 512
MvaI CCWGG 2 cut(s) 36, 512
MwoI GCNNNNNNNGC 4 cut(s) 24, 45, 154, 470
NciI CCSGG 1 cut(s) 470
NdeII GATC 2 cut(s) 379, 478
NlaIII CATG 2 cut(s) 52, 127
NlaIV GGNNCC 2 cut(s) 40, 149
NmuCI GTSAC 1 cut(s) 367
NspI RCATGY 1 cut(s) 52
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 1 cut(s) 31
PfoI TCCNGGA 1 cut(s) 510
PkrI GCNGC 1 cut(s) 475
PsiI TTATAA 1 cut(s) 140
Psp6I CCWGG 2 cut(s) 34, 510
PspGI CCWGG 2 cut(s) 34, 510
PspN4I GGNNCC 2 cut(s) 40, 149
PsuI RGATCY 1 cut(s) 379
PvuII CAGCTG 2 cut(s) 410, 476
RsaI GTAC 3 cut(s) 182, 250, 260
RsaNI GTAC 3 cut(s) 181, 249, 259
SalI GTCGAC 1 cut(s) 391
SatI GCNGC 1 cut(s) 474
Sau3AI GATC 2 cut(s) 379, 478
ScrFI CCNGG 3 cut(s) 36, 470, 512
SduI GDGCHC 2 cut(s) 150, 441
SfaNI GCATC 1 cut(s) 577
SfcI CTRYAG 1 cut(s) 12
Sse9I AATT 5 cut(s) 141, 171, 224, 229, 357
StyD4I CCNGG 3 cut(s) 34, 468, 510
TaaI ACNGT 2 cut(s) 209, 488
TaiI ACGT 4 cut(s) 317, 327, 392, 498
TaqI TCGA 1 cut(s) 392
TasI AATT 5 cut(s) 141, 171, 224, 229, 357
TatI WGTACW 2 cut(s) 180, 258
TfiI GAWTC 1 cut(s) 31
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 1 cut(s) 473
Tsp45I GTSAC 1 cut(s) 367
TspRI CASTG 1 cut(s) 340
XapI RAATTY 1 cut(s) 171
XceI RCATGY 1 cut(s) 52
XmiI GTMKAC 1 cut(s) 392
XmnI GAANNNNTTC 1 cut(s) 305
ZraI GACGTC 2 cut(s) 325, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.